Control method and device of air displacement type pipette pump, pipette pump and storage medium

Abstract

The invention relates to the technical field of experimental instruments, and discloses a control method and device of an air replacement type pipette pump, the pipette pump and a storage medium, and the method comprises the following steps: obtaining the current temperature and target volume of a target sample; determining a target unit volume adjustment coefficient of the target sample based on the current temperature; the unit volume adjustment coefficient changes along with different temperatures of the target sample and different evaporation rates of the target sample at different temperatures; determining an adjustment volume according to the target unit volume adjustment coefficient and the target volume; generating a pipetting control instruction based on the adjustment volume; and controlling the pipetting operation of the target sample according to the control instruction. According to the invention, the pipetting amount error of the air displacement type pipetting pump can be reduced, especially the error when the pipetting amount is low, and the precision of the pipetting amount is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of experimental instruments, and in particular to a control method and device of an air displacement type liquid transfer pump, a liquid transfer pump and a storage medium. Background Art

[0002] The purpose of automatic micro-liquid sampling is to accurately complete the operation of liquid aspiration and dispensing. Among them, air displacement pipetting technology has low cross-contamination between samples and is easily compatible with automated pipetting workstations. It is increasingly becoming an effective way to perform micro-sampling in fields such as molecular diagnosis. The air displacement pipette is a commonly used laboratory equipment that uses air displacement pipetting technology. It uses an air column to isolate the liquid and the mechanical parts inside the pipette to avoid contamination and cross-contamination.

[0003] Air displacement pipette pumps include the process of aspirating and discharging liquid, which involves the interaction between gas and liquid phases. Especially when the volume of liquid transfer reaches less than 5μL, small external changes can easily cause a significant increase in the allowable error of the volume of liquid transfer. How to analyze the main influencing factors under low liquid transfer volume and then construct a method to compensate for the volume with multiple factors is extremely critical to reducing the allowable error of the volume of liquid transfer. Summary of the invention

[0004] In view of this, the present invention provides a control method and device for an air displacement type pipette pump, a pipette pump and a storage medium to solve the problem of large errors in the air displacement type pipette pump.

[0005] In a first aspect, the present invention provides a method for controlling an air displacement type pipette pump, the method comprising:

[0006] Obtain the current temperature and target volume of the target sample;

[0007] Determining a target unit volume adjustment coefficient of the target sample based on the current temperature; the unit volume adjustment coefficient varies with the temperature of the target sample and the evaporation rate of the target sample at different temperatures;

[0008] determining an adjusted volume according to the target unit volume adjustment coefficient and the target volume;

[0009] generating a pipetting control instruction based on the adjusted volume;

[0010] The pipetting operation of the target sample is controlled according to the control instruction.

[0011] In an optional embodiment, generating a pipetting control instruction based on the adjusted volume includes:

[0012] A control instruction of a pipetting motor of the air displacement pipetting pump is generated based on the adjustment volume, wherein the control instruction is used to control the operation time and / or step length of the pipetting motor.

[0013] In an optional embodiment, the generating of the control instruction of the pipetting motor of the air displacement pipetting pump based on the adjusted volume comprises:

[0014] adjusting the step length of the pipetting motor according to the difference between the adjusted volume and the target volume;

[0015] Generate a corresponding control instruction based on the adjusted step size.

[0016] In an optional embodiment, adjusting the step length of the pipetting motor according to the difference between the adjustment volume and the target volume includes:

[0017] Determining an adjustment value of the step length of the pipetting motor based on a difference between the adjustment volume and the target volume;

[0018] Determining the adjusted step length based on the default value of the step length of the pipetting motor and the adjustment value;

[0019] Among them, the calculation formula of the adjustment value is: ΔN=(ΔV / V0-d) / E, ΔN is the adjustment value, ΔV is the difference between the adjustment volume and the target volume, V0 is the target volume, and E and d are parameter values ​​that change with the temperature and evaporation rate of the target sample.

[0020] In an optional implementation, determining the target unit volume adjustment coefficient of the target sample based on the current temperature includes:

[0021] Obtaining a functional relationship between a unit volume adjustment coefficient of the target sample and a sample temperature; the functional relationship is a quadratic functional relationship;

[0022] Based on the functional relationship, the target unit volume adjustment coefficient corresponding to the current temperature is determined.

[0023] In an optional implementation, obtaining the functional relationship between the unit volume adjustment coefficient of the target sample and the temperature of the sample includes:

[0024] Determine the sample type to which the target sample belongs;

[0025] Acquire the functional relationship corresponding to the sample type to which the target sample belongs.

[0026] In a second aspect, the present invention provides a control device for an air displacement type pipette pump, the device comprising:

[0027] A temperature and volume acquisition module is used to obtain the current temperature and target volume of the target sample;

[0028] An adjustment coefficient determination module, configured to determine a target unit volume adjustment coefficient of the target sample based on the current temperature; the unit volume adjustment coefficient varies with different sample temperatures and different evaporation rates of the sample at different sample temperatures;

[0029] an adjustment volume determination module, configured to determine an adjustment volume according to the target unit volume adjustment coefficient and the target volume;

[0030] A generating module, configured to generate a pipetting control instruction based on the adjusted volume;

[0031] The pipetting control module is used to control the pipetting operation of the target sample according to the control instruction.

[0032] In a third aspect, the present invention provides an air displacement type liquid transfer pump, comprising: a control host and a liquid transfer actuator;

[0033] The control host includes: a memory and a processor, the memory and the processor are communicatively connected to each other, the memory stores computer instructions, and the processor executes the control method of the air displacement pipette pump of the first aspect or any corresponding embodiment thereof by executing the computer instructions.

[0034] In a fourth aspect, the present invention provides a computer-readable storage medium having computer instructions stored thereon, the computer instructions being used to enable a computer to execute the control method for an air displacement pipette pump according to the first aspect or any corresponding embodiment thereof.

[0035] In a fifth aspect, the present invention provides a computer program product, comprising computer instructions for causing a computer to execute the control method of an air displacement pipette pump according to the first aspect or any corresponding embodiment thereof. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the related technologies, the drawings required for use in the specific embodiments or the related technical descriptions will be briefly introduced below. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0037] Figure 1 is a schematic diagram of the principle of how temperature and sample evaporation affect sample volume according to an embodiment of the present invention;

[0038] Figure 2is a curve schematic diagram showing the effects of temperature and evaporation on sample volume according to an embodiment of the present invention;

[0039] Figure 3 It is a curve diagram showing the linear effect of a single temperature field on the sample volume;

[0040] Figure 4 is a flow chart of a control method of an air displacement type pipetting pump according to an embodiment of the present invention;

[0041] Figure 5 is a schematic diagram of different adjustment volumes at different sample temperatures according to an embodiment of the present invention;

[0042] Figure 6 is a schematic diagram of a process for determining formula parameter analysis according to an embodiment of the present invention;

[0043] Figure 7 is a structural block diagram of a control device for an air displacement type pipetting pump according to an embodiment of the present invention;

[0044] Figure 8 Schematic diagram of the hardware structure of the air displacement pipette pump according to an embodiment of the present invention. DETAILED DESCRIPTION

[0045] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0046] The internal flow channel of the air displacement pipette pump is mainly a gas-liquid two-phase field. The interaction between temperature and air pressure in the pipette head affects its working process. The fluid (i.e., the sample) follows the law of conservation of mass, momentum, and energy in the flow channel. The energy transfer during the pipetting process mainly occurs between the air and the liquid. In the actual pipetting process, especially when a small amount of sample is pipetted, the sample temperature and sample evaporation will significantly affect the pipetting process and change the pipetting results. Figure 1 As shown, the present invention further incorporates the calculation and analysis of the sample evaporation process based on the consideration of temperature, thereby obtaining a nonlinear volume change curve (such as Figure 2 Compared with the case where only a single temperature change factor is considered ( Figure 3 The single temperature field linear influence curve is shown) showing different characteristics, and a control method is established based on the change curve to improve the accuracy and stability of the air displacement pipette pump.

[0047] According to an embodiment of the present invention, a control method embodiment of an air displacement pipette pump is provided. It should be noted that the steps shown in the flowchart of the accompanying drawings can be executed in a computer system such as a set of executable computer instructions, and although a logical order is shown in the flowchart, in some cases, the steps shown or described can be executed in an order different from that shown here.

[0048] In this embodiment, a control method for an air displacement type pipette pump is provided. Figure 4 is a flow chart of a control method of an air displacement type pipetting pump according to an embodiment of the present invention. Figure 4 As shown, the process includes the following steps:

[0049] Step S401, obtaining the current temperature and target volume of the target sample.

[0050] Specifically, the target sample is the sample that currently needs to be pipetted, and the target volume is the volume of the target sample that is currently needed.

[0051] Step S402, determining a target unit volume adjustment coefficient of the target sample based on the current temperature; the unit volume adjustment coefficient varies with the temperature of the target sample and the evaporation rate of the target sample at different temperatures.

[0052] Specifically, step S402, i.e., determining the target unit volume adjustment coefficient of the target sample based on the current temperature, includes:

[0053] Step S4021, obtaining the functional relationship between the unit volume adjustment coefficient of the target sample and the sample temperature; the functional relationship is a quadratic functional relationship; for example, the quadratic functional relationship is expressed as: β V =-aT 2 -bT+c, where a, b, and c are coefficients greater than 0. Figure 2 The figure shows a schematic diagram of a curve of the change of the adjusted volume with the sample temperature of 1 μL of the target sample at the standard temperature (here 21°) after adding the sample evaporation analysis. The curve is consistent with the curve corresponding to the functional relationship between the unit volume adjustment coefficient of the target sample after adding the sample evaporation analysis and the sample temperature. Figure 2 The quadratic function relationship corresponding to the curve shown in is: 调 =-0.01615T 2 -4.61795×10 -4 T+1.5471, Figure 5 The figure shows a schematic diagram of the adjusted volumes at different sample temperatures calculated based on the relationship for the same target volume of the sample.

[0054] Step S4022: determining the target unit volume adjustment coefficient corresponding to the current temperature based on the functional relationship.

[0055] In the embodiment of the present invention, when constructing the functional relationship between the unit volume adjustment coefficient of the target sample and the sample temperature, the analysis of the evaporation of the target sample is added, so that the temperature change and volume change of the sample no longer present Figure 3 The linear change shown is not Figure 2 The nonlinear change shown in the figure shows that the obtained temperature-volume curve conforms to the change trend of the quadratic function curve, and the obtained fitting degree R 2 ≈0.9998.

[0056] In some optional specific implementations, step S4021, obtaining a functional relationship between the unit volume adjustment coefficient of the target sample and the temperature of the sample, includes:

[0057] Step S40211, determining the sample type to which the target sample belongs;

[0058] Step S40212: Obtain the functional relationship corresponding to the sample type to which the target sample belongs.

[0059] Among them, the sample types can be classified according to evaporation rate or according to composition.

[0060] In the embodiment of the present invention, the corresponding functional relationship is determined according to the sample type described by the target sample, so as to improve the accuracy of the target unit volume adjustment coefficient of the target sample, thereby further improving the accuracy of the pipetting amount.

[0061] Step S403: determining an adjustment volume according to the target unit volume adjustment coefficient and the target volume.

[0062] In some optional specific implementations, the target unit volume adjustment coefficient may be directly multiplied by the target volume to obtain the adjusted volume.

[0063] In the embodiment of the present invention, the functional relationship between the unit volume adjustment coefficient of the target sample and the sample temperature can be obtained based on a fixed ambient temperature, such as room temperature 25° C. If the ambient temperature is not this temperature, the volume deviation caused by the change in ambient temperature needs to be considered to further improve the accuracy of the pipetting amount.

[0064] Step S404, namely generating a pipetting control instruction based on the adjusted volume.

[0065] In the embodiment of the present invention, the air displacement type pipetting pump performs the pipetting operation according to the adjusted volume, that is, the adjusted volume is used as the pipetting volume.

[0066] In some optional specific implementations, step S404, i.e., generating a pipetting control instruction based on the adjusted volume, includes:

[0067] A control instruction of a pipetting motor of the air displacement pipetting pump is generated based on the adjustment volume, wherein the control instruction is used to control the operation time and / or step length of the pipetting motor.

[0068] Specifically, the generating of the control instruction of the pipetting motor of the air displacement pipetting pump based on the adjusted volume includes:

[0069] Step 1: adjusting the step length of the pipetting motor according to the difference between the adjusted volume and the target volume.

[0070] In some optional specific embodiments, the step size of the pipetting motor can be adjusted according to the ratio of the difference to the target volume.

[0071] In some other optional specific implementations, adjusting the step length of the pipetting motor according to the difference between the adjusted volume and the target volume includes:

[0072] Determining an adjustment value of the step length of the pipetting motor based on a difference between the adjustment volume and the target volume;

[0073] Determining the adjusted step length based on the default value of the step length of the pipetting motor and the adjustment value;

[0074] The calculation formula of the adjustment value is: ΔN=(ΔV / V0-d) / E, ΔN is the adjustment value, ΔV is the difference between the adjustment volume and the target volume, V0 is the target volume, and E and d are parameter values ​​that change with the temperature and evaporation rate of the target sample. Adjustment volume V 调 The calculation method is: V 调 =β v ×V0.

[0075] Step 2: Generate a corresponding control instruction based on the adjusted step length. In other embodiments, a corresponding control instruction may also be generated based on the adjusted value of the step length, which is the step length adjustment control instruction. The adjusted value of the step length is the difference between the adjusted step length and the default step length.

[0076] In the embodiment of the present invention, if the liquid transfer motor of the liquid transfer pump is a stepping motor, the sample volume can be adjusted by adjusting the step length of the stepping motor. In addition, regardless of whether the liquid transfer motor of the liquid transfer pump is a stepping motor or not, the sample volume can be adjusted by adjusting the running time of the liquid transfer motor.

[0077] In the embodiment of the present invention, adaptive control of the step length of the pipetting motor of the air displacement pipetting pump can be achieved by inputting the target volume of the sample and the current temperature of the sample, thereby completing nonlinear precise control of the pipetting volume of the sample.

[0078] The above formula β v =-aT 2 The parameters a, b, c in -bT+, and the parameters E and d in ΔN=(ΔV / V0-d) / E are obtained by fitting the fluid temperature in the analysis method. Specifically, multiple analyses are required, and the sample temperature is different each time. Please refer to the analysis process for each time. Figure 6 ( Figure 6 The fluid temperature in the sample is the sample temperature). In the example of the present invention, when establishing an analysis method for the influence of temperature and evaporation rate on the transfer volume, an axisymmetric two-dimensional model with a pipette tip as a template is constructed, water is selected as the transfer sample, the transfer target volume is 1 μL, the ambient temperature is set to 21°C, the ambient pressure is standard atmospheric pressure, air is selected as the gas, and the parameters of the fluid such as constant pressure heat capacity, density, thermal conductivity, etc. are set as functions of the temperature as the dependent variable. Subsequently, only the parameters such as the fluid material, ambient temperature, and sample temperature need to be changed to obtain the temperature-volume fitting curves of different types of samples affected by temperature and evaporation rate based on this analysis method, and the functional relationship between the unit volume adjustment coefficient of the sample and the sample temperature can be obtained based on the curve.

[0079] Step S405 , controlling the pipetting operation of the target sample according to the control instruction.

[0080] The contact between samples (liquids) of different temperatures and air causes the volume of the air to change, that is, the air contracts when it is cold and expands when it is heated, which in turn affects the air pressure in the closed chamber of the pipette pump and changes the volume of the liquid to be pipetted. During the pipetting process, the temperature difference between the sample, the ambient air and the pipette head will cause the sample evaporation rate to change. For example, when transferring low-temperature samples, the sample evaporation rate will be accelerated, the gas concentration in the closed chamber will increase, the pipetting process will be inhibited, and the accuracy of the pipetting results will be affected. When transferring low-temperature and volatile samples, the error of pipetting will be magnified.

[0081] The control method of the air displacement pipette pump provided in the present embodiment breaks through the previous method of ignoring the evaporation of liquid in the closed cavity and only considering the influence of the temperature difference between the sample and the environment on the pipetting result. A nonlinear influence curve different from the calculation of a single temperature factor is obtained, and a pipetting volume control method is designed based on the influence curve to achieve precise control of different temperatures under low pipetting volumes. This is of great significance for solving the problem that the air displacement pipette pump is affected by different factors when transferring ultra-low trace samples, resulting in nonlinear changes in the pipetting results and excessive allowable capacity errors.

[0082] In this embodiment, a control device for an air displacement type pipette pump is also provided, which is used to implement the above-mentioned embodiments and preferred embodiments, and will not be repeated hereafter. As used below, the term "module" can be a combination of software and / or hardware that implements a predetermined function. Although the devices described in the following embodiments are preferably implemented in software, the implementation of hardware, or a combination of software and hardware, is also possible and conceivable.

[0083] This embodiment provides a control device for an air displacement type liquid transfer pump, such as Figure 7 As shown, including:

[0084] The temperature and volume acquisition module 601 is used to acquire the current temperature and target volume of the target sample;

[0085] An adjustment coefficient determination module 602 is used to determine a target unit volume adjustment coefficient of the target sample based on the current temperature; the unit volume adjustment coefficient varies with different sample temperatures and different evaporation rates of the sample at different sample temperatures;

[0086] An adjustment volume determination module 603, configured to determine an adjustment volume according to the target unit volume adjustment coefficient and the target volume;

[0087] A generating module 604, configured to generate a pipetting control instruction based on the adjusted volume;

[0088] The pipetting control module 605 is used to control the pipetting operation of the target sample according to the control instruction.

[0089] In some optional embodiments, the generation module 604 is specifically used to generate a control instruction for the pipetting motor of the air displacement pipetting pump based on the adjusted volume, and the control instruction is used to control the operating time and / or step length of the pipetting motor.

[0090] In some optional implementations, the generating module 604 includes:

[0091] a step length adjustment unit, used for adjusting the step length of the pipetting motor according to the difference between the adjustment volume and the target volume;

[0092] An instruction generating unit is used to generate corresponding control instructions based on the adjusted step size.

[0093] In some optional implementations, the step size adjustment unit includes:

[0094] a step length adjustment value determination subunit, configured to determine an adjustment value of the step length of the pipetting motor based on a difference between the adjustment volume and the target volume;

[0095] an adjusted step length determining unit, configured to determine the adjusted step length based on a default value of the step length of the pipetting motor and the adjusted value;

[0096] Among them, the calculation formula of the adjustment value is: ΔN=(ΔV / V0-d) / E, ΔN is the adjustment value, ΔV is the difference between the adjustment volume and the target volume, V0 is the target volume, and E and d are parameter values ​​that change with the temperature and evaporation rate of the target sample.

[0097] In some optional implementations, the adjustment coefficient determination module 602 includes:

[0098] A functional relationship acquisition unit, used to acquire a functional relationship between a unit volume adjustment coefficient of the target sample and a sample temperature; the functional relationship is a quadratic functional relationship;

[0099] A coefficient determination unit is used to determine the target unit volume adjustment coefficient corresponding to the current temperature based on the functional relationship.

[0100] In some optional implementations, the functional relationship acquisition unit includes:

[0101] A judging subunit, used to judge the sample type to which the target sample belongs;

[0102] The acquisition subunit is used to acquire the functional relationship corresponding to the sample type to which the target sample belongs.

[0103] The further functional description of each of the above modules and units is the same as that of the above corresponding embodiments and will not be repeated here.

[0104] The control device of the air displacement pipette pump in this embodiment is presented in the form of a functional unit, where the unit refers to an ASIC (Application Specific Integrated Circuit) circuit, a processor and memory that executes one or more software or fixed programs, and / or other devices that can provide the above functions.

[0105] The embodiment of the present invention also provides an air displacement type liquid transfer pump, comprising a control host and a liquid transfer actuator, wherein the control host has the above Figure 7 The control device of the air displacement type liquid transfer pump shown in the figure. The liquid transfer actuator includes a piston movement module and a closed chamber module. The air displacement type liquid transfer pump also includes a data acquisition module, etc.

[0106] See also Figure 8 , Figure 8 FIG. 1 is a schematic diagram of the structure of an air displacement type liquid transfer pump provided by an optional embodiment of the present invention (the liquid transfer actuator is not shown in the figure), such as Figure 8As shown, the control host of the air displacement type liquid transfer pump includes: one or more processors 10, a memory 20, and an interface for connecting various components, including a high-speed interface and a low-speed interface. Each component utilizes different buses to communicate with each other, and can be installed on a common mainboard or installed in other ways as needed. The processor can process the instructions executed in the air displacement type liquid transfer pump, including instructions stored in or on the memory to display the graphical information of the GUI on an external input / output device (such as, a display device coupled to the interface). In some optional embodiments, if necessary, multiple processors and / or multiple buses can be used together with multiple memories and multiple memories. Figure 8 A processor 10 is taken as an example.

[0107] The processor 10 may be a central processing unit, a network processor or a combination thereof. The processor 10 may further include a hardware chip. The hardware chip may be a dedicated integrated circuit, a programmable logic device or a combination thereof. The programmable logic device may be a complex programmable logic device, a field programmable gate array, a general purpose array logic or any combination thereof.

[0108] The memory 20 stores instructions executable by at least one processor 10, so that the at least one processor 10 executes the method shown in the above embodiment.

[0109] The memory 20 may include a program storage area and a data storage area, wherein the program storage area may store an operating system and an application required by at least one function; the data storage area may store data created according to the use of the air displacement type pipette pump, etc. In addition, the memory 20 may include a high-speed random access memory, and may also include a non-transient memory, such as at least one disk storage device, a flash memory device, or other non-transient solid-state storage device. In some optional embodiments, the memory 20 may optionally include a memory remotely arranged relative to the processor 10, and these remote memories may be connected to the air displacement type pipette pump via a network. Examples of the above-mentioned network include, but are not limited to, the Internet, an intranet, a local area network, a mobile communication network, and combinations thereof.

[0110] The memory 20 may include a volatile memory, such as a random access memory; the memory may also include a non-volatile memory, such as a flash memory, a hard disk or a solid state drive; the memory 20 may also include a combination of the above types of memory.

[0111] The air displacement pipette pump further includes an input device 30 and an output device 40. The processor 10, the memory 20, the input device 30 and the output device 40 may be connected via a bus or other means. Figure 8 The example of connecting through bus is taken in the following.

[0112] The input device 30 can receive input digital or character information, and generate key signal input related to the user settings and function control of the air displacement pipette pump, such as a touch screen, a keypad, a mouse, a track pad, a touch pad, an indicator rod, one or more mouse buttons, a trackball, a joystick, etc. The output device 40 may include a display device, an auxiliary lighting device (e.g., an LED) and a tactile feedback device (e.g., a vibration motor), etc. The above-mentioned display device includes but is not limited to a liquid crystal display, a light emitting diode, a display and a plasma display. In some optional embodiments, the display device can be a touch screen.

[0113] The air displacement type pipette pump further comprises a communication interface for the air displacement type pipette pump to communicate with other devices or a communication network.

[0114] The embodiment of the present invention also provides a computer-readable storage medium. The method according to the embodiment of the present invention can be implemented in hardware, firmware, or can be implemented as a computer code that can be recorded in a storage medium, or can be implemented as a computer code that is originally stored in a remote storage medium or a non-temporary machine-readable storage medium and will be stored in a local storage medium through a network download, so that the method described herein can be stored in such software processing on a storage medium using a general-purpose computer, a dedicated processor, or programmable or dedicated hardware. Among them, the storage medium can be a magnetic disk, an optical disk, a read-only storage memory, a random access memory, a flash memory, a hard disk or a solid-state hard disk, etc.; further, the storage medium can also include a combination of the above types of memories. It can be understood that a computer, a processor, a microprocessor controller, or programmable hardware includes a storage component that can store or receive software or computer code. When the software or computer code is accessed and executed by a computer, a processor, or hardware, the method shown in the above embodiment is implemented.

[0115] A part of the present invention may be applied as a computer program product, such as a computer program instruction, which, when executed by a computer, can call or provide the method and / or technical solution according to the present invention through the operation of the computer. Those skilled in the art should understand that the existence of the computer program instruction in a computer-readable medium includes, but is not limited to, a source file, an executable file, an installation package file, etc., and accordingly, the way in which the computer program instruction is executed by the computer includes, but is not limited to: the computer directly executes the instruction, or the computer compiles the instruction and then executes the corresponding compiled program, or the computer reads and executes the instruction, or the computer reads and installs the instruction and then executes the corresponding installed program. Here, the computer-readable medium may be any available computer-readable storage medium or communication medium accessible to the computer.

[0116] Although the embodiments of the present invention have been described in conjunction with the accompanying drawings, those skilled in the art may make various modifications and variations without departing from the spirit and scope of the present invention, and such modifications and variations are all within the scope defined by the appended claims.

Claims

1. A control method for an air displacement type pipette pump, characterized in that: The method comprises: Obtain the current temperature and target volume of the target sample; Determining a target unit volume adjustment coefficient of the target sample based on the current temperature; the unit volume adjustment coefficient varies with the temperature of the target sample and the evaporation rate of the target sample at different temperatures; determining an adjusted volume according to the target unit volume adjustment coefficient and the target volume; generating a pipetting control instruction based on the adjusted volume; The pipetting operation of the target sample is controlled according to the control instruction.

2. The method according to claim 1, characterized in that The step of generating a pipetting control instruction based on the adjusted volume comprises: A control instruction of a pipetting motor of the air displacement pipetting pump is generated based on the adjustment volume, wherein the control instruction is used to control the operation time and / or step length of the pipetting motor.

3. The method according to claim 2, characterized in that The step of generating a control instruction for a pipetting motor of the air displacement pipetting pump based on the adjusted volume comprises: adjusting the step length of the pipetting motor according to the difference between the adjusted volume and the target volume; Generate a corresponding control instruction based on the adjusted step size.

4. The method according to claim 3, characterized in that: The stepping of adjusting the step length of the pipetting motor according to the difference between the adjusted volume and the target volume comprises: Determining an adjustment value of the step length of the pipetting motor based on a difference between the adjustment volume and the target volume; Determining the adjusted step length based on the default value of the step length of the pipetting motor and the adjustment value; Among them, the calculation formula of the adjustment value is: ΔN=(ΔV / V0-d) / E, ΔN is the adjustment value, ΔV is the difference between the adjustment volume and the target volume, V0 is the target volume, and E and d are parameter values ​​that change with the temperature and evaporation rate of the target sample.

5. The method according to claim 1, characterized in that The determining the target unit volume adjustment coefficient of the target sample based on the current temperature includes: Obtaining a functional relationship between a unit volume adjustment coefficient of the target sample and a sample temperature; the functional relationship is a quadratic functional relationship; Based on the functional relationship, the target unit volume adjustment coefficient corresponding to the current temperature is determined.

6. The method according to claim 5, characterized in that The step of obtaining a functional relationship between a unit volume adjustment coefficient of the target sample and a temperature of the sample includes: Determine the sample type to which the target sample belongs; Acquire the functional relationship corresponding to the sample type to which the target sample belongs.

7. A control device for an air displacement type pipette pump, characterized in that: The device comprises: A temperature and volume acquisition module is used to obtain the current temperature and target volume of the target sample; An adjustment coefficient determination module, configured to determine a target unit volume adjustment coefficient of the target sample based on the current temperature; the unit volume adjustment coefficient varies with different sample temperatures and different evaporation rates of the sample at different sample temperatures; an adjustment volume determination module, configured to determine an adjustment volume according to the target unit volume adjustment coefficient and the target volume; A generating module, configured to generate a pipetting control instruction based on the adjusted volume; The pipetting control module is used to control the pipetting operation of the target sample according to the control instruction.

8. An air displacement type pipetting pump, characterized in that: include: Control host and pipetting actuator; The control host includes a memory and a processor, the memory and the processor are communicatively connected to each other, the memory stores computer instructions, and the processor executes the control method of the air displacement pipette pump according to any one of claims 1 to 6 by executing the computer instructions.

9. A computer-readable storage medium, characterized in that: The computer-readable storage medium stores computer instructions, and the computer instructions are used to enable a computer to execute the control method of the air displacement pipette pump according to any one of claims 1 to 6.

10. A computer program product, characterized in that The method comprises computer instructions for causing a computer to execute the control method of the air displacement type pipetting pump according to any one of claims 1 to 6.

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