Amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate

Abstract

The invention discloses an amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate. The amino acid organic water-soluble fertilizer is obtained by encapsulating earthworm enzymatic hydrolysate stock solution and nitrogen phosphorus and potassium organic materials in microgel, the microgel is obtained by cross-linking chitosan quaternary ammonium salt and cellulose through a cross-linking agent. The quaternary ammonium salt structure with positive charges in the microgel has the effects of dispersion, stabilization and bacteriostasis, the organic water-soluble fertilizer has the slow release effect after being encapsulated, loss of the fertilizer is prevented, the particle size of the obtained microgel is 1-20 microns, and the microgel is convenient to spray.

Description

Technical Field

[0001] The invention belongs to the technical field of water-soluble fertilizers, and in particular relates to an amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate. Background Art

[0002] There are more than ten kinds of proteolytic enzymes in earthworms. At the suitable temperature of 50-60℃ and pH=7.0-7.5, earthworms can use their own enzymes to perform enzymatic hydrolysis. The resulting enzymatic solution is rich in nutrients, mainly including 18 kinds of free amino acids, short peptides, small molecular proteins, and chelated trace elements such as iron, copper, manganese and zinc. These ingredients have obvious effects on increasing crop yields, improving the quality of agricultural products, and enhancing plant disease resistance. Therefore, earthworm enzymatic hydrolysate has been developed into a variety of amino acid fertilizers.

[0003] At present, organic water-soluble fertilizers mainly have the following problems: 1) Although organic fertilizers have a longer action time than inorganic fertilizers, in general, traditional fertilizers have the problem of insufficient action time, resulting in the need for multiple fertilization; 2) Deterioration caused by incomplete inactivation of microbial contamination and reproduction. Microgels are colloidal particles with a certain three-dimensional network structure formed by cross-linking of biopolymer molecules. The mutual connection between polymer molecules mainly includes covalent bonds, van der Waals forces, hydrophobic interactions, hydrogen bonds and ionic bonds. Through the protective effect of the microgel matrix, the active substances can be effectively isolated from the external environment, thereby greatly reducing the risk of denaturation and inactivation of active substances due to environmental changes, and can also achieve their slow release. Chitosan is a natural polysaccharide with a structure similar to that of cellulose. It has good biocompatibility and biodegradability and can be used as a suitable modified substrate for microgels. However, due to its large molecular weight, it is easy to form strong hydrogen bonds within and between molecules, and it is difficult to dissolve in water and organic solvents, which greatly limits its application. Microcapsule solutions have the problem of unstable dispersion, which may lead to precipitation or stratification, resulting in blockage during spraying and affecting fertilization efficiency. Summary of the invention

[0004] In order to overcome the shortcomings of the above-mentioned prior art, the present invention provides an amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate encapsulated in microgel. The microgel is obtained by cross-linking chitosan quaternary ammonium salt and cellulose through a cross-linking agent. The microgel carries a positive charge and plays a role of dispersion and stabilization. The organic water-soluble fertilizer plays a sustained-release role after being encapsulated to prevent the loss of the fertilizer. The obtained microgel particle size is 1 to 20 μm, and it is easy to spray.

[0005] The technical scheme for achieving the purpose of the present invention is as follows: an amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate, characterized in that the amino acid organic water-soluble fertilizer is obtained by encapsulating earthworm enzymatic hydrolysate stock solution and nitrogen, phosphorus and potassium organic materials in microgel; the preparation method of the earthworm enzymatic hydrolysate stock solution is as follows:

[0006] S1. Clean the fresh earthworms, remove impurities, empty the viscera, drain the water and then freeze them quickly;

[0007] S2. The obtained frozen earthworms are enzymatically hydrolyzed in a water bath at a temperature of 45 to 55°C and a pH of 7.0 to 7.5 for 4 to 5 hours;

[0008] S3. After centrifugation, 1 to 2 wt% of a water-soluble mold inhibitor is added to the obtained supernatant to obtain an earthworm enzymatic hydrolysis solution;

[0009] The microgel is obtained by cross-linking chitosan quaternary ammonium salt and cellulose with a cross-linking agent at pH=5.0-9.0; the particle size of the microgel is 1-20 μm.

[0010] The resulting solids can be recovered as feed.

[0011] Preferably, the nitrogen, phosphorus and potassium organic material is obtained by one or more methods of composting and fermentation, hydrothermal treatment after pyrolysis and microbial transformation of agricultural waste; the agricultural waste is at least one of wheat straw, rice straw, corn straw, vegetable leaves, fruit tree branches and rice husks.

[0012] Preferably, the quick freezing temperature in step S1 is -20 to -10°C; the enzyme required for enzymatic hydrolysis in step S2 is the proteolytic enzyme in the earthworm; and the centrifugal speed in step S3 is 1000 to 2000 rpm.

[0013] Preferably, the preparation method of the chitosan quaternary ammonium salt is as follows:

[0014] 0.2 parts of chitosan and 8-10 parts of 1-allyl-3-methylimidazolium chloride ionic liquid are added into a three-necked flask, heated in an oil bath at 75-85° C. with stirring until the chitosan is completely dissolved, 0.4-0.6 parts of glycidyltrimethylammonium chloride are added into the flask and stirred at 75-85° C. for 7-8 hours, a mixed solution of ethanol and acetone is added into the flask to precipitate chitosan quaternary ammonium salt, the mixed solution is filtered to obtain a chitosan quaternary ammonium salt filter cake, the filter cake is washed at least twice with a mixed solution of ethanol and acetone to remove 1-allyl-3-methylimidazolium chloride and glycidyltrimethylammonium chloride, and the chitosan quaternary ammonium salt is dried.

[0015] Preferably, the cellulose is bacterial cellulose; and the cross-linking agent is sodium tetraborate.

[0016] Preferably, the preparation method of the amino acid organic water-soluble fertilizer is as follows:

[0017] 1) After diluting the earthworm enzymatic hydrolysis stock solution, the nitrogen, phosphorus and potassium organic materials are dispersed therein and ultrasonicated for 5 to 10 minutes. After adjusting the pH value to 5.0 to 9.0, chitosan quaternary ammonium salt and cellulose are added and stirred for 10 to 20 minutes. The cross-linking agent dissolved in water is slowly added dropwise to the system and taken out after stirring for 10 to 16 hours;

[0018] 2) After homogenizing with a homogenizer for 2 to 3 minutes, the microgel suspension is subjected to ultrasonic crushing for 8 to 10 minutes, the pH value is adjusted, and the suspension is sieved through a 200 to 300 mesh screen to obtain an amino acid organic water-soluble fertilizer.

[0019] Preferably, the earthworm enzymatic hydrolysis stock solution in step 1) is diluted 300 to 500 times; the mass ratio of the chitosan quaternary ammonium salt, cellulose and cross-linking agent is (8 to 10): 1: (8 to 20); the total mass of the chitosan quaternary ammonium salt, cellulose and cross-linking agent is 15 to 20 w.t% of the amino acid organic water-soluble fertilizer; the mass of the nitrogen, phosphorus and potassium organic material is 5 to 10 w.t% of the amino acid organic water-soluble fertilizer.

[0020] Preferably, the speed of the homogenizer in step 2) is 8000-10000 rpm; the power of the ultrasonic crushing is 360-400 W, and the mixture is run for 3s and stopped for 3s in an ice bath; and the pH value is 4-10.

[0021] Beneficial Effects

[0022] The present invention has the following beneficial effects: the present invention provides an amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate with high stability, good slow-release effect, antibacterial and antiseptic effects. The effective components of the amino acid organic water-soluble fertilizer are encapsulated by the action of hydrogen bonds and ionic bonds in the microgel, and the slow-release rate is controlled by adjusting the pH value; the quaternary ammonium salt structure makes the microgel encapsulating the amino acid organic water-soluble fertilizer positively charged as a whole, and is not easy to agglomerate in water, thereby improving the dispersibility and stability of the amino acid organic water-soluble fertilizer, preventing the blockage of the nozzle or pipeline, and ensuring the normal operation of the fertilization system; the quaternary ammonium salt has a certain antibacterial effect, further inhibiting the reproduction of bacteria, fungi and algae that may exist in the water-soluble fertilizer, extending the shelf life of the fertilizer, and preventing the fertilizer from deteriorating. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The preparation route of the microgel of the present invention;

[0024] Figure 2 The infrared spectra of chitosan, chitosan quaternary ammonium salt and microgel of the present invention are shown.

[0025] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] In the examples, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified.

[0027] The raw materials and equipment used in the embodiments and comparative examples are described as follows:

[0028] Water-soluble mold inhibitor: main ingredients: aflatoxin B1 decomposing mold, Bacillus subtilis, Bacillus licheniformis, lactic acid bacteria, biological complex enzyme, glucose oxidase, purchased from Xinweimu;

[0029] Chitosan: deacetylation degree ≥95%, product number A001827, purchased from Jianglai Biotechnology;

[0030] 1-Allyl-3-methylimidazole chloride: Product No. 1039111, purchased from Shanghai Haohong Biopharmaceutical Technology;

[0031] Epoxypropyltrimethylammonium chloride: Product No. 1228799, purchased from Shanghai Haohong Biopharmaceutical Technology;

[0032] Cellulose: bacterial cellulose, diameter 50-100 nm, purchased from Guilin Qihong Technology Co., Ltd.;

[0033] Chitosan quaternary ammonium salt:

[0034] 0.2 parts of chitosan and 8 parts of 1-allyl-3-methylimidazolium chloride ionic liquid were added into a three-necked flask, heated and stirred at 80°C until the chitosan was completely dissolved, 0.4 parts of glycidyl trimethylammonium chloride was added into the flask and stirred at 80°C for 8h, a mixed solution of ethanol and acetone was added into the flask to precipitate chitosan quaternary ammonium salt, the mixed solution was filtered to obtain a chitosan quaternary ammonium salt filter cake, washed 3 times with a mixed solution of ethanol and acetone to remove 1-allyl-3-methylimidazolium chloride and glycidyl trimethylammonium chloride, and dried to obtain chitosan quaternary ammonium salt;

[0035] JIUPIN-1200E ultrasonic crusher: Wuxi Jiuping Instrument Co., Ltd.

[0036] Earthworm enzymatic hydrolysis solution

[0037] S1. Clean the fresh earthworms, remove impurities, empty the viscera, drain the water and then freeze them at -20°C;

[0038] S2. The obtained frozen earthworms were enzymatically hydrolyzed in a water bath at a temperature of 50°C and a pH of 7.0 for 4 h;

[0039] S3. After centrifugation at a centrifugal speed of 1000 rpm, 1.5 wt% of a water-soluble mold inhibitor was added to the obtained supernatant to obtain an earthworm enzymatic hydrolysis stock solution.

[0040] A method for preparing amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate

[0041] Example 1

[0042] 1) After diluting the earthworm enzymatic hydrolysis stock solution by 400 times, 8wt% of nitrogen, phosphorus and potassium organic materials of the total weight of the amino acid organic water-soluble fertilizer are dispersed therein, ultrasonically treated for 5 minutes, the pH is adjusted to 9.0, chitosan quaternary ammonium salt and cellulose are added, stirred for 10 minutes, and the cross-linking agent dissolved in water is slowly added dropwise to the system, the mass ratio of chitosan quaternary ammonium salt, cellulose and cross-linking agent is 9:1:8, and the total weight of chitosan quaternary ammonium salt, cellulose and cross-linking agent is 18wt% of the amino acid organic water-soluble fertilizer; after stirring for 12 hours, the system is taken out;

[0043] 2) After homogenizing for 2 minutes at a homogenizer speed of 8000 rpm, the microgel suspension was subjected to ultrasonic crushing for 8 to 10 minutes, the pH value was adjusted to 6.5, and the suspension was sieved through a 300-mesh sieve to obtain an amino acid organic water-soluble fertilizer.

[0044] Example 2

[0045] Compared with the preparation method in Example 1, the difference is that in step 1), the mass ratio of chitosan quaternary ammonium salt, cellulose and cross-linking agent is 9:1:16.

[0046] Example 3

[0047] Compared with the preparation method in Example 1, the difference is that in step 1), the mass ratio of chitosan quaternary ammonium salt, cellulose and cross-linking agent is 9:1:20.

[0048] Example 4

[0049] Compared with the preparation method in Example 1, the difference is that in step 2), the speed of the homogenizer is replaced from 8000 rpm to 9000 rpm.

[0050] Example 5

[0051] Compared with the preparation method in Example 1, the difference is that in step 2), the speed of the homogenizer is replaced from 8000 rpm to 10000 rpm.

[0052] Example 6

[0053] Compared with the preparation method in Example 1, the difference is that step 1) adjusting the pH to 9.0 is replaced by adjusting the pH to 7.

[0054] Example 7

[0055] Compared with the preparation method in Example 1, the difference is that step 1) adjusting the pH to 9.0 is replaced by adjusting the pH to 5.

[0056] Comparative Example 1

[0057] Compared with the preparation method in Example 1, the difference is that in step 1), the chitosan quaternary ammonium salt is replaced by chitosan.

[0058] Comparative Example 2

[0059] Compared with the preparation method in Example 1, the difference is that in step 2), no homogenization and crushing treatment is performed.

[0060] Comparative Example 3

[0061] The preparation method is as follows: after diluting the earthworm enzymatic hydrolysis stock solution by 400 times, nitrogen, phosphorus and potassium organic materials are dispersed therein and ultrasonicated for 5 minutes to obtain amino acid organic water-soluble fertilizer.

[0062] Comparative Example 4

[0063] Compared with the preparation method in Example 1, the difference is that step 1) adjusting the pH to 9.0 is replaced by adjusting the pH to 11.0.

[0064] Comparative Example 5

[0065] Compared with the preparation method in Example 1, the difference is that step 1) adjusting the pH to 9 is replaced by adjusting the pH to 3.0.

[0066] The following is the test method of the performance parameters involved in the present invention:

[0067] Fourier transform infrared spectroscopy (FTIR) determination: model Spectrum two, PerkinElmer, USA;

[0068] Particle size: S3500 laser particle size analyzer, Microtrac, USA. Particle size test is the test data before sieving.

[0069] Release rate: Collect the same batch of air-dried soil and place them in containers of 15cm*15cm*15cm respectively. Spray 10ml of organic water-soluble fertilizer on the soil surface and keep the soil moisture at 60%. Place it at room temperature. Use a sampler with a diameter of 0.5cm and a height of 15cm to sample the soil on days 1, 10, 20 and 30 respectively. After extraction with aqueous solution, test it according to the industry standard NTY1975-2010 "Determination of the content of free amino acids in water-soluble fertilizers".

[0070] Antibacterial activity test: The inoculated Staphylococcus aureus and Escherichia coli were transferred to a constant temperature shaker at 37°C, 130r, and cultured for 18h to 24h, and the bacterial solution was diluted to 3×105CFU / mL to 4×105CFU / mL with 0.03mol / L phosphate (PBS) buffer; 0.2g of the sample of the embodiment and the comparative example was added to a conical flask, and 100μL of the diluted bacterial solution was added and cultured at 24°C, 150r / min for 18h to 24h; the bacterial solution after shaking was diluted by 10-fold dilution method, and 100μL of the bacterial solution was applied to the agar plate for each dilution gradient, and two plates were plated in parallel for each dilution gradient. After culturing at 37°C for 24h, the colonies on the plates were counted, and the control group without any component added was used; the antibacterial rate was calculated by formula (1).

[0071]

[0072] Where: E anti is the antibacterial rate; N c is the colony count of the control group; N e It is the colony count of embodiment and comparative example test.

[0073] Dispersion stability test: Place for 180 days to observe whether it is stable and whether there is turbidity or precipitation.

[0074] Field fertilizer effect experiment of tomatoes: Using the same fertilizer amount and frequency, the V c The yield increase rate compared with the application of an equal amount of clean water was measured and statistically analyzed.

[0075] By the attached Figure 2 It can be seen that the infrared spectra of quaternary ammonium salt chitosan and chitosan are not much different. Quaternary ammonium salt chitosan has a peak at 1653 cm -1 and 1483cm -1 There are two strong peaks, the former is the stretching movement of NH of tertiary amine, and the latter is the stretching movement of CH in -N(CH3)3; in the spectrum of microgel, 1670cm -1 It is the stretching motion of C=O. The vibration peak of -OH changes from 3441cm -1 Red shifted to 3272 cm -1 , 1323cm -1 and 690cm -1 It is the stretching vibration of BOB and the bending vibration of BOC.

[0076] Table 1 Average particle size and antibacterial activity determination of examples and comparative examples

[0077]

[0078]

[0079] It can be seen from the data in Table 1 that the particle size, antibacterial rate, sustained-release effect and dispersion stability of Examples 1 to 7 are all very excellent; it can be seen from the data in Examples 1 to 3 that with the increase in the amount of cross-linking agent used, the particle size of the microcapsules encapsulating the amino acid organic water-soluble fertilizer first increases and then decreases, and the amino acid release rate also first decreases and then increases; this is because with the increase in cross-linking density, the network structure of the microcapsules is tighter, and the loading capacity for amino acids is improved, but when the microgel network density is too high, the steric hindrance increases, resulting in a decrease in the encapsulation rate and thus an increase in the release rate; it can be seen from the data in Examples 1, 4, and 5 that the greater the speed of the homogenizer, the smaller the particle size of the microcapsules, the slower the release rate, and during the homogenization process, the shear Shear force can refine the microgel and reduce the particle size. The slower the release rate is because the homogenization improves the dispersibility of the microgel. It can be seen from the data of Examples 1, 6, and 7 that when the pH is between 5 and 9, the higher the pH, the lower the release rate. When the pH is 9.0, most of the amino acids in the earthworm are negatively charged. At this time, the adsorption capacity of chitosan quaternary ammonium salt for amino acids is higher, so the encapsulation rate is higher. When the pH is 5, the negative charge of the amino acids decreases, and the adsorption capacity of polysaccharide quaternary ammonium salt for amino acids decreases, so the encapsulation rate decreases. It can be seen from the data of Comparative Example 1 that the dispersion stability and antibacterial properties of the microgel after chitosan quaternary ammonium salt is replaced by chitosan are not as good as those of Examples 1 to 7, and the release rate is slightly increased. It can be seen from the data of Comparative Example 2 that the particle size of the gel without homogenization and crushing treatment is too large, not within the range of 1 to 20 μm, and is therefore not suitable for the spray system; since Comparative Example 3 was not encapsulated, no sustained release and particle size tests were performed; it can be seen from Comparative Examples 4 to 5 that too high or too low pH will reduce the encapsulation rate and increase the release rate. When the pH is too high, the positive charge of the chitosan quaternary ammonium salt decreases, and the dispersibility is also reduced; when the pH is too low, some amino acids begin to carry a positive charge, resulting in a decrease in the encapsulation rate.

[0080] Table 2 Field fertilizer efficiency experiments corresponding to the embodiments and comparative examples

[0081]

[0082]

[0083] From the fertilizer efficiency test data in Table 2, it can be seen that for tomatoes, a good slow-release effect is very necessary for the gain of fertilizer efficiency. From the data of Examples 1 to 7 and Comparative Examples 1, 4, and 5, it can be seen that within a certain range, as the slow-release effect increases, the V c The sugar-acid ratio and the fruit yield rate are gradually increased. From the data of comparative example 1, it can be seen that directly spraying unencapsulated amino acid organic water-soluble fertilizer can also increase the V c, sugar-acid ratio and yield, but the effect is not as good as that of Examples 1 to 7 of the present invention; since the particle size of Comparative Example 2 is too large and is not suitable for the spraying system, no experimental control is performed.

[0084] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. The preferred embodiments do not describe all the details in detail, nor do they limit the invention to the specific implementation methods described. Obviously, many modifications and changes can be made according to the content of this specification. This specification selects and specifically describes these embodiments in order to better explain the principles and practical applications of the present invention, so that those skilled in the art can understand and use the present invention well. The present invention is limited only by the claims and their full scope and equivalents.

Claims

1. An amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate, characterized in that: The amino acid organic water-soluble fertilizer is obtained by encapsulating earthworm enzymatic hydrolysis stock solution and nitrogen, phosphorus and potassium organic materials in microgel; the preparation method of the earthworm enzymatic hydrolysis stock solution is as follows: S1. Clean the fresh earthworms, remove impurities, empty the viscera, drain the water and then freeze them quickly; S2. The obtained frozen earthworms are enzymatically hydrolyzed in a water bath at a temperature of 45 to 55°C and a pH of 7.0 to 7.5 for 4 to 5 hours; S3. After centrifugation, a water-soluble mold inhibitor is added to the obtained supernatant to obtain an earthworm enzymatic hydrolysis solution; The microgel is obtained by cross-linking chitosan quaternary ammonium salt and cellulose with a cross-linking agent at pH=5.0-9.0; the particle size of the microgel is 1-20 μm.

2. The amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate according to claim 1, characterized in that: The nitrogen, phosphorus and potassium organic materials are obtained by one or more methods of composting and fermentation, hydrothermal treatment after pyrolysis and microbial transformation of agricultural waste; the agricultural waste is at least one of wheat straw, rice straw, corn straw, vegetable leaves, fruit tree branches and rice husks.

3. The amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate according to claim 1, characterized in that: The quick freezing temperature in step S1 is -20 to -10°C; the enzyme required for enzymatic hydrolysis in step S2 is the proteolytic enzyme in the earthworm; the centrifugal speed in step S3 is 1000 to 2000 rpm, and the added amount of the water-soluble mold inhibitor is 1 to 2 w.t%.

4. The amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate according to claim 1, characterized in that: The chitosan quaternary ammonium salt preparation method is as follows: Chitosan and solvent are added into a reaction kettle, heated and stirred until chitosan is completely dissolved, epoxypropyltrimethylammonium chloride is added into the reaction kettle and stirred, and after the reaction is completed, post-treatment is performed to obtain chitosan quaternary ammonium salt.

5. The amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate according to claim 4, characterized in that: The mass ratio of chitosan, solvent and glycidyltrimethylammonium chloride is 1:(40-50):(2-3); the stirring temperature is 75-85°C and the time is 7-8h; the solvent is 1-allyl-3-methylimidazolium chloride ionic liquid; the post-treatment operation is to add a mixed solution of ethanol and acetone into a flask to precipitate chitosan quaternary ammonium salt, filter the mixed solution to obtain a chitosan quaternary ammonium salt filter cake, wash it with a mixed solution of ethanol and acetone for at least 2 times to remove impurities, and dry it to obtain the chitosan quaternary ammonium salt.

6. The amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate according to claim 1, characterized in that: The cellulose is bacterial cellulose; and the cross-linking agent is sodium tetraborate.

7. An amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate according to any one of claims 1 to 6, characterized in that: The preparation method of the amino acid organic water-soluble fertilizer is as follows: 1) After diluting the earthworm enzymatic hydrolysis stock solution, the nitrogen, phosphorus and potassium organic materials are dispersed therein and ultrasonicated for 5 to 10 minutes. After adjusting the pH value to 5.0 to 9.0, chitosan quaternary ammonium salt and cellulose are added and stirred for 10 to 20 minutes. The cross-linking agent dissolved in water is slowly added dropwise to the system and taken out after stirring for 10 to 16 hours; 2) After homogenizing with a homogenizer for 2 to 3 minutes, the microgel suspension is subjected to ultrasonic crushing for 8 to 10 minutes, the pH value is adjusted, and the obtained water-soluble fertilizer is sieved through a 200 to 300 mesh screen to obtain an amino acid organic water-soluble fertilizer.

8. The method for preparing an amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate according to claim 7, characterized in that: The earthworm enzymatic hydrolysis solution in step 1) is diluted 300 to 500 times; the mass ratio of the chitosan quaternary ammonium salt, cellulose and cross-linking agent is (8 to 10): 1: (8 to 20); the total mass of the chitosan quaternary ammonium salt, cellulose and cross-linking agent is 15 to 20 w.t% of the amino acid organic water-soluble fertilizer; the mass of the nitrogen, phosphorus and potassium organic material is 5 to 10 w.t% of the amino acid organic water-soluble fertilizer.

9. The method for preparing an amino acid organic water-soluble fertilizer based on earthworm enzymatic hydrolysate according to claim 7, characterized in that: The speed of the homogenizer in step 2) is 8000-10000 rpm; the power of the ultrasonic crushing is 360-400 W, and the mixture is run for 3 seconds and stopped for 3 seconds in an ice bath; and the pH value is 4-10.

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