Tremella aurantialba liquid strain preparation device and preparation method thereof

Abstract

The invention relates to the technical field of strain preparation, in particular to a tremella aurantialba liquid strain preparation device and a preparation method thereof. The invention provides a tremella aurantialba liquid strain preparation device with an improved structure and improved functions. The device comprises a control box body, a rotary shaking assembly, a gravity center adjusting assembly and the like, the control box body is internally provided with the rotary shaking assembly for oscillating and shaking the liquid strain, and the constant-temperature water tank is internally provided with the gravity center adjusting assembly for adjusting the oscillation amplitude of the liquid strain. Through cooperation of the rotary shaking assembly and the up-down oscillation assembly, up-down oscillation can be synchronously performed while rotary shaking is performed on the prepared tremella aurantialba liquid strain, so that the efficiency of fully shaking up the tremella aurantialba liquid strain is improved; the adjusting arm is horizontally moved along the guide rail to adjust the position, so that the gravity center positions of the conical flask and the test tube containing the tremella aurantialba liquid strain can be flexibly adjusted, and the amplitude of rotating and shaking up the tremella aurantialba liquid strain can be improved to the greatest extent.

Description

Technical Field

[0001] The invention relates to the technical field of strain preparation, in particular to a device for preparing a Tremella fuciformis liquid strain and a preparation method thereof. Background Art

[0002] Golden ear is an edible fungus named for its golden color and beautiful shape. Golden ear liquid culture refers to a high concentration mycelium suspension formed by culturing the mycelium of golden ear in liquid culture medium.

[0003] An invention patent for a water bath oscillator is disclosed on the patent website, and its authorization announcement number is CN220559057U. The device includes a body, a shaker is arranged inside the body, and both sides of the shaker are connected to the body through oscillation arms. A plurality of groups of clamp arms are arranged above the shaker, and a torsion spring is connected to the inner bottom of the clamp arm. The clamp arm is rotatably connected to the shaker through the torsion spring. The clamp arm is "S"-shaped, and a silicone pad is arranged on the inner side of the clamp arm. A silicone ring is arranged on the inner side of the bottom of each group of clamp arms, and the silicone ring has a low peripheral height in shape. Although the device can clamp and fix various types of reaction containers of different specifications through the fixing structure of the "S"-shaped clamp arm and the torsion spring, the fixing structure is relatively simple and has poor stability, so that the clamped container is easily unstable in gravity and tilted to a large extent under the action of eccentricity and inertia during the swirling shaking process, which may cause collisions between the containers or overflow of the liquid in the container; and the shaker does not have the function of shaking the liquid in the container up and down synchronously, which leads to low efficiency in shaking the liquid.

[0004] In order to overcome the above-mentioned deficiencies, a device for preparing agaric liquid fungus is particularly proposed, which can effectively improve the efficiency of liquid shaking and firmly clamp the container. Summary of the invention

[0005] In order to overcome the shortcomings of the existing device during use, the technical problem of the present invention is to provide a Tremella fuciformis liquid spawn preparation device with improved structure and function.

[0006] A device for preparing golden ear liquid spawn comprises a base, a control box and a constant temperature water tank, wherein the base is provided with a control box, and also comprises a gyratory shaking component and a center of gravity adjusting component, wherein the control box is provided with a gyratory shaking component for oscillating and shaking the liquid spawn, wherein the gyratory shaking component comprises a driving motor, a rocker arm 1, a connecting frame, a rocker arm 2 and an upper and lower oscillating member, wherein the driving motor is installed at the bottom of the control box body, and the driving motor is electrically connected to an adjusting knob of the control box body, the output shaft of the driving motor is connected with a rocker arm 1, and the four corners of the bottom of the control box body are rotatably connected with the rocker arm 2 respectively, a connecting frame is rotatably connected between the upper ends of the rocker arm 1 and the rocker arm 2, the upper part of the connecting frame is connected with a constant temperature water tank for maintaining the preparation temperature of the liquid spawn through the upper and lower oscillating members, and the constant temperature water tank is provided with a center of gravity adjusting component for adjusting the oscillation amplitude of the liquid spawn.

[0007] Furthermore, the upper and lower oscillating parts include a connecting block, a connecting shaft, a guide sleeve, an elastic part and a connecting frame. The connecting frame is provided with a guide sleeve corresponding to the second rocker arm, the sliding sleeve outside the guide sleeve is provided with a connecting block, a movable hole is opened on the connecting block, a connecting shaft is connected in the movable hole, and the connecting shaft movable sleeve is arranged inside the guide sleeve, and an elastic part is connected between the connecting shaft and the bottom of the guide sleeve, a connecting frame is connected between the upper ends of the connecting blocks, and the constant temperature water tank is arranged on the connecting frame.

[0008] Furthermore, the center of gravity adjustment component includes a guide rail, an adjustment arm, a locking bolt and a clamping piece. The guide rails are symmetrically arranged on the upper inner side of the constant temperature water tank. Both guide rails are slidably sleeved with adjustment arms that can horizontally move and adjust the position. The adjustment arm is threadedly provided with a locking bolt near one end of the guide rail, and the lower end of the locking bolt can abut against the guide rail. The adjustment arm is connected with a clamping piece for clamping and limiting the conical flask and test tube containing liquid bacteria.

[0009] Furthermore, the clamping member includes a fixed arc block, a movable arc block, a locking column, a rubber block, a positioning structure and a placing member. The fixed arc block is connected between the two adjusting arms, and one end of the fixed arc block is threadedly connected to the movable arc block through the locking column. Through the clamping cooperation of the fixed arc block and the movable arc block, the conical bottle can be clamped and fixed. Rubber blocks are provided on the inner sides of the fixed arc block and the movable arc block, and a positioning structure is provided on the end of the fixed arc block and the movable arc block opposite to the locking column.

[0010] Furthermore, the placement piece includes a catheter rack, a connecting rod and a support ring. The front and rear sides of the two adjustment arms are provided with a catheter rack for housing the test tube. A connecting rod is provided at the bottom of each catheter rack, and a support ring is connected to the lower end of each connecting rod.

[0011] Furthermore, the positioning structure is mainly composed of a positioning rod and a positioning hole that are plugged into each other, and the positioning rod is arranged on the movable arc block, and the fixed arc block is provided with a positioning hole that is compatible with the positioning rod.

[0012] Furthermore, it also includes a lifting piece, which includes a guide rod, a tray and a threaded shaft. The right adjusting arm is connected to the guide rod, the tray is slidably sleeved on the guide rod, the right adjusting arm is rotatably sleeved on the threaded shaft, and the tray is threadedly sleeved on the threaded shaft.

[0013] Furthermore, a protective cover is also included, and a protective cover capable of adaptively retracting and folding is connected between the outer side of the upper part of the control box body and the outer side of the bottom of the constant temperature water tank.

[0014] In addition, the present invention also provides a method for preparing a liquid fungus strain of Tremella fuciformis, as shown below:

[0015] S1. Cut the Tremella tissue blocks into small thin pieces and add them into liquid culture medium. The culture medium formula is 10g corn flour, 3g peptone, 3g potassium dihydrogen phosphate, 1.5g magnesium sulfate, and 1000ml water;

[0016] S2, culturing in a constant temperature water bath at a temperature of 20°C-24°C for 3-8 days, and performing shake bottle fermentation on the Tremella fuciformis liquid strain using a shaking device;

[0017] S3, packing the treated Tremella fuciformis liquid strain into sterile test tubes, filtering them aseptically and sealing them;

[0018] S4, complete the preparation of Tremella fuciformis liquid culture.

[0019] The beneficial effects of the present invention are as follows: through the cooperation of the swirling shaking component and the up and down oscillation component, the prepared golden ear liquid strain can be swirled and shaken while being oscillated up and down synchronously, which is beneficial to improving the efficiency of fully shaking the golden ear liquid strain; by adjusting the position of the adjusting arm horizontally along the guide rail, the center of gravity position of the conical flask and test tube containing the golden ear liquid strain can be flexibly adjusted, which is beneficial to maximizing the amplitude of the swirling shaking of the golden ear liquid strain, and thus is beneficial to improving the preparation efficiency of the golden ear liquid strain. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention.

[0021] Figure 2 It is a cross-sectional view of the present invention.

[0022] Figure 3 This is a diagram showing the sealing cover plate of the present invention in an open state.

[0023] Figure 4 It is a schematic diagram of the connection structure of the gyratory shaking component, the up and down oscillation component and the connection frame of the present invention.

[0024] Figure 5 It is a schematic diagram of the connection structure of the gyratory shaking component and the up and down oscillation component of the present invention.

[0025] Figure 6 It is a partial cross-sectional view of the upper and lower oscillating components of the present invention.

[0026] Figure 7 It is a schematic diagram of the three-dimensional structure of the present invention.

[0027] Figure 8 This is a structural separation diagram of the center of gravity adjustment component and the clamping member of the present invention.

[0028] Fig. 9 This is a structural separation diagram of the clamping member of the present invention.

[0029] In the above drawings: 1: base, 2: control box, 3: drive motor, 31: rocker one, 32: connecting frame, 33: rocker two, 4: connecting block, 41: movable hole, 42: connecting shaft, 43: guide sleeve, 44: elastic part, 5: connecting frame, 6: constant temperature water tank, 60: drain valve, 61: observation window, 62: sealing cover, 7: heating tube, 8: guide rail, 81: adjustment arm, 82: locking bolt, 83: fixed arc block, 831: movable arc block, 832: locking column, 833: rubber block, 834: positioning rod, 835: positioning hole, 84: guide rod, 841: tray, 842: threaded shaft, 9: catheter rack, 91: connecting rod, 92: support ring, 10: protective sleeve, a: conical flask, b: test tube. DETAILED DESCRIPTION

[0030] The present invention will be further described below in conjunction with specific embodiments. The illustrative embodiments and descriptions of the present invention are used to explain the present invention but are not intended to limit the present invention.

[0031] Embodiment: The present invention provides a device for preparing a liquid fungus strain of Tremella fuciformis, such as Figures 1 to 9As shown, it includes a base 1, a control box 2, a constant temperature water tank 6, a drain valve 60, an observation window 61, a sealing cover 62, a heating tube 7, a swirling shaking component and a center of gravity adjustment component. The base 1 is provided with a control box 2, and a swirling shaking component is provided inside the control box 2. The swirling shaking component includes a driving motor 3, a rocker 1 31, a connecting frame 32, a rocker 2 33 and an upper and lower oscillating member. The driving motor 3 is installed at the bottom of the control box 2, and the output shaft of the driving motor 3 is connected to the rocker 1 31. The four corners of the bottom of the control box 2 are rotatably connected to the rocker 2 33, and the rotation radius values ​​of the rocker 1 31 and the rocker 2 33 are equal, so that the rocker 1 31 and the rocker 2 33 can rotate and swing synchronously, and the upper ends of the rocker 1 31 and the rocker 2 33 rotate. The thermostatic water tank 6 is dynamically connected to a connecting frame 32, and the upper part of the connecting frame 32 is connected to a constant temperature water tank 6 through an upper and lower oscillating member. A heating tube 7 is arranged at the bottom of the thermostatic water tank 6, and the heating tube 7 and the driving motor 3 are electrically connected to the adjusting knob on the control box 2 respectively. A sealing cover plate 62 is embedded in the top of the thermostatic water tank 6, and an observation window 61 is arranged on the front side of the thermostatic water tank 6. The observation window 61 is made of transparent glass material, which is convenient for observing the preparation of the bacteria inside the thermostatic water tank 6 in real time. The outer side of the bottom of the thermostatic water tank 6 is connected and communicated with a drain valve 60, which is convenient for discharging the liquid inside the thermostatic water tank 6 after use. A center of gravity adjustment component is arranged inside the thermostatic water tank 6, and the center of gravity adjustment component can adjust the amplitude of the shaking oscillation of the bacteria to the greatest extent, thereby helping to improve the efficiency of shaking the bacteria.

[0032] Further, such as Figure 2 , Figure 4 , Figure 5 and Figure 6 As shown, the up and down oscillating parts include a connecting block 4, a connecting shaft 42, a guide sleeve 43, an elastic member 44 and a connecting frame 5. A guide sleeve 43 corresponding to the rocker arm 2 33 is provided on the connecting frame 32. The outer sliding sleeve of the guide sleeve 43 is provided with a connecting block 4, and the guide sleeve 43 can be used to guide the connecting block 4 vertically. A movable hole 41 is opened on the connecting block 4, and a connecting shaft 42 is connected to the movable hole 41. The connecting shaft 42 is movably sleeved inside the guide sleeve 43, and an elastic member 44 is connected between the connecting shaft 42 and the inner bottom of the guide sleeve 43. The elastic member 44 is a compression spring, which is used to make the connecting shaft 42 and the connecting block 4 thereon oscillate up and down. A connecting frame 5 is connected between the upper ends of the connecting blocks 4, and a constant temperature water tank 6 is arranged on the connecting frame 5.

[0033] Further, such as Figure 2 , Figure 3 , Figure 7 , Figure 8 and Fig. 9As shown, the center of gravity adjustment component includes a guide rail 8, an adjustment arm 81, a locking bolt 82 and a clamping piece. The guide rails 8 are symmetrically arranged on the upper inner side of the constant temperature water tank 6. The two guide rails 8 are slidably sleeved with adjustment arms 81. The locking bolt 82 is threadedly penetrated on one end of the adjustment arm 81 close to the guide rail 8, and the lower end of the locking bolt 82 can abut against the guide rail 8. The adjustment arm 81 is connected with a clamping piece for clamping and fixing the conical flask a and the test tube b. By adjusting the position of the adjustment arm 81 by horizontal movement, the center of gravity of the conical flask a and the test tube b containing the golden ear liquid culture can be conveniently adjusted accordingly, so that the shaking amplitude of the conical flask a and the test tube b can be adjusted to the maximum extent, which is beneficial to improving the preparation efficiency of the golden ear liquid culture.

[0034] Further, such as Figure 7 , Figure 8 and Fig. 9 As shown, the clamping member includes a fixed arc block 83, a movable arc block 831, a locking column 832, a rubber block 833, a positioning structure and a placement member. The fixed arc block 83 is connected between the two adjustment arms 81. One end of the fixed arc block 83 is threadedly connected to the movable arc block 831 through the locking column 832. Through the clamping cooperation of the fixed arc block 83 and the movable arc block 831, the conical bottle a can be clamped and fixed. The inner sides of the fixed arc block 83 and the movable arc block 831 are both provided with rubber blocks 833, which is conducive to improving the In order to ensure the stability of the conical bottle a and avoid tilting and shifting during shaking, a positioning structure is provided on the fixed arc block 83 and the movable arc block 831 at the end opposite to the locking column 832; the positioning structure is mainly composed of a positioning rod 834 and a positioning hole 835 that are plugged in and matched with each other, and the positioning rod 834 is provided on the movable arc block 831, and a positioning hole 835 that is compatible with the positioning rod 834 is opened on the fixed arc block 83, which is beneficial to improving the stability of the cooperation between the movable arc block 831 and the fixed arc block 83.

[0035] Further, such as Figure 3 , Figure 7 and Figure 8 As shown, the placement member includes a catheter rack 9, a connecting rod 91 and a support ring 92. The front and rear sides of the two adjustment arms 81 are provided with a catheter rack 9 for placing the test tube b. The bottom of each catheter rack 9 is provided with a connecting rod 91. The lower end of each connecting rod 91 is connected to a support ring 92. The support ring 92 can be used to lift and position the bottom of the test tube b placed thereon, which is beneficial to ensure the stability of the placement of the test tube b.

[0036] Further, such as Figure 7 and Figure 8As shown, it also includes a lifting piece, which includes a guide rod 84, a tray 841 and a threaded shaft 842. The right adjusting arm 81 is connected to the guide rod 84, and the tray 841 is slidably sleeved on the guide rod 84. The right adjusting arm 81 is rotatably sleeved with a threaded shaft 842, and the tray 841 is threadedly sleeved on the threaded shaft 842. By adjusting the threaded shaft 842, the height of the tray 841 can be adjusted accordingly, which is beneficial to the purpose of stably lifting the bottom of the conical flask a.

[0037] Further, such as Figure 1 , Figure 2 and Figure 3 As shown, a protective cover 10 is also included. The protective cover 10 that can adaptively retract and fold is connected between the upper outer side of the control box 2 and the bottom outer side of the constant temperature water tank 6. The protective cover 10 can provide safety protection for the interior of the control box 2 to avoid potential safety hazards.

[0038] When it is necessary to prepare the golden ear liquid strain, add an appropriate amount of the golden ear liquid strain to a container containing sterile water for suspension. After a certain proportion is prepared, pour the golden ear liquid strain into the conical flask a containing the composite culture medium, then remove the sealing cover plate 62, add a certain amount of clean water into the constant temperature water tank 6, and then place the conical flask a on the tray 841. By adjusting the threaded shaft 842, the height of the conical flask a can be adjusted accordingly. By adjusting the locking column 832, the clamping of the movable arc block 831 and the fixed arc block 83 can be adjusted. The function is to clamp and fix the neck of the conical flask a, which is beneficial to maintain the stability of the conical flask a during the shaking and oscillation process and avoid displacement; at the same time, different types of golden ear liquid bacteria with a good proportion can be packed into test tubes b, and the test tubes b are inserted into the corresponding catheter rack 9, and the bottom of the test tube b placed thereon can be lifted and positioned by the support ring 92; in addition, according to the amount of golden ear liquid bacteria preparation capacity, the adjustment arm 81 can be adjusted to different positions on the guide rail 8 by horizontal movement, so as to adjust the shaking and oscillation process. The center of gravity of the golden ear liquid strain is flexibly adjusted accordingly, so that the golden ear liquid strain can be shaken and oscillated to the maximum extent, which is conducive to fully shaking the golden ear liquid strain in a short time, thereby helping to improve the preparation efficiency of the golden ear liquid strain; after the above steps are completed, cover the sealing cover 62; start the adjustment knob on the control box 2, and the drive motor 3 will drive the constant temperature water tank 6 thereon to swing in a whirlpool and shake up and down, so that the golden ear liquid strain in the conical flask a and the test tube b can be shaken and processed, and at the same time The water in the constant temperature water tank 6 can be heated to a predetermined temperature and maintained at a constant temperature through the heating tube 7, which is beneficial to provide a good ambient temperature for the golden ear liquid strain and to improve the quality of the preparation of the golden ear liquid strain. During the preparation of the golden ear liquid strain, the preparation status of the golden ear liquid strain can be viewed in real time through the observation window 61. After the golden ear liquid strain is fully shaken, the adjusting knob on the control box 2 is closed, the conical flask a and the test tube b are taken out and aseptically sealed. In this way, the preparation of the golden ear liquid strain can be completed.

[0039] In addition, the present invention also provides a method for preparing a liquid fungus strain of Tremella fuciformis, as shown below:

[0040] S1. Cut the Tremella tissue blocks into small thin pieces and add them into liquid culture medium. The culture medium formula is 10g corn flour, 3g peptone, 3g potassium dihydrogen phosphate, 1.5g magnesium sulfate, and 1000ml water;

[0041] S2, culturing in a constant temperature water bath at a temperature of 20°C-24°C for 3-8 days, and performing shake bottle fermentation on the Tremella fuciformis liquid strain using a shaking device;

[0042] S3, packing the treated Tremella fuciformis liquid strain into sterile test tubes, filtering them aseptically and sealing them;

[0043] S4, complete the preparation of Tremella fuciformis liquid culture.

[0044] It should be understood that the above description is only for exemplary purposes and is not meant to limit the present invention. Those skilled in the art will appreciate that variations of the present invention will be included within the scope of the claims herein.

Claims

1. A Tremella fuciformis liquid strain preparation device, comprising a base (1), a control box (2) and a constant temperature water tank (6), wherein the base (1) is provided with a control box (2), and wherein: The invention also comprises a gyratory shaking component and a center of gravity adjustment component. The control box (2) is provided with a gyratory shaking component for oscillating and shaking the liquid bacteria. The gyratory shaking component comprises a driving motor (3), a rocker arm 1 (31), a connecting frame (32), a rocker arm 2 (33) and an upper and lower oscillating member. The driving motor (3) is installed at the bottom of the control box (2), and the driving motor (3) is electrically connected to the adjustment knob of the control box (2). The output shaft of the driving motor (3) is connected to the rocker arm 1 (31). The four corners of the bottom of the control box (2) are rotatably connected to the rocker arm 2 (33). The connecting frame (32) is rotatably connected between the upper ends of the rocker arm 1 (31) and the rocker arm 2 (33). The upper part of the connecting frame (32) is connected to a constant temperature water tank (6) for maintaining the liquid bacteria preparation temperature through the upper and lower oscillating members. The constant temperature water tank (6) is provided with a center of gravity adjustment component for adjusting the oscillation amplitude of the liquid bacteria.

2. A Tremella fuciformis liquid strain preparation device according to claim 1, characterized in that: The up-and-down oscillating member comprises a connecting block (4), a connecting shaft (42), a guide sleeve (43), an elastic member (44) and a connecting frame (5); the connecting frame (32) is provided with a guide sleeve (43) corresponding to the rocker arm 2 (33); the outer sliding sleeve of the guide sleeve (43) is provided with a connecting block (4); a movable hole (41) is provided on the connecting block (4); the movable hole (41) is connected with a connecting shaft (42); the connecting shaft (42) is movably sleeved inside the guide sleeve (43); the elastic member (44) is connected between the connecting shaft (42) and the inner bottom of the guide sleeve (43); the upper end of the connecting block (4) is connected with a connecting frame (5); and the constant temperature water tank (6) is arranged on the connecting frame (5).

3. A Tremella fuciformis liquid strain preparation device according to claim 1, characterized in that: The center of gravity adjustment component comprises a guide rail (8), an adjustment arm (81), a locking bolt (82) and a clamping piece. The guide rail (8) is symmetrically arranged on the upper inner side of the constant temperature water tank (6). The two guide rails (8) are both slidably sleeved with an adjustment arm (81) capable of horizontally moving and adjusting the position. The locking bolt (82) is threadedly penetrated at one end of the adjustment arm (8) close to the guide rail (8), and the lower end of the locking bolt (82) can abut against the guide rail (8). The adjustment arm (81) is connected with a clamping piece for clamping and limiting the conical flask (a) and the test tube (b) containing liquid bacteria.

4. A Tremella fuciformis liquid strain preparation device according to claim 3, characterized in that: The clamping member comprises a fixed arc block (83), a movable arc block (831), a locking column (832), a rubber block (833), a positioning structure and a placing member. The fixed arc block (83) is connected between the two adjusting arms (81). One end of the fixed arc block (83) is threadedly connected to the movable arc block (831) through the locking column (832). Through the clamping cooperation of the fixed arc block (83) and the movable arc block (831), the conical bottle (a) can be clamped and fixed. The inner sides of the fixed arc block (83) and the movable arc block (831) are both provided with a rubber block (833). The fixed arc block (83) and the movable arc block (831) are provided with a positioning structure at one end opposite to the locking column (832).

5. A Tremella fuciformis liquid strain preparation device according to claim 4, characterized in that: The placement member comprises a catheter rack (9), a connecting rod (91) and a support ring (92); the front and rear sides of the two adjustment arms (81) are each provided with a catheter rack (9) for housing the test tube (b); a connecting rod (91) is provided at the bottom of each catheter rack (9); and the lower end of each connecting rod (91) is connected to a support ring (92).

6. A Tremella fuciformis liquid strain preparation device according to claim 4, characterized in that: The positioning structure is mainly composed of a positioning rod (834) and a positioning hole (835) that are plugged into each other, and the positioning rod (834) is arranged on the movable arc block (831), and the fixed arc block (83) is provided with a positioning hole (835) that is compatible with the positioning rod (834).

7. The device for preparing Tremella fuciformis liquid strain according to claim 3, characterized in that: The invention also comprises a lifting member, wherein the lifting member comprises a guide rod (84), a tray (841) and a threaded shaft (842); the right adjusting arm (81) is connected with the guide rod (84); the tray (841) is slidably sleeved on the guide rod (84); the right adjusting arm (81) is rotatably sleeved with the threaded shaft (842); and the tray (841) is threadedly sleeved on the threaded shaft (842).

8. The apparatus for preparing Tremella fuciformis liquid strain according to claim 1, characterized in that: It also includes a protective cover (10), and the protective cover (10) capable of adaptively telescoping and folding is connected between the upper outer side of the control box body (2) and the bottom outer side of the constant temperature water tank (6).

9. A device for preparing Tremella fuciformis liquid strains according to any one of claims 1 to 8, characterized in that: A method for preparing a Tremella fuciformis liquid strain is as follows: S1. Cut the Tremella tissue block into small thin pieces and add them into liquid culture medium. The culture medium formula is 10g corn flour, 3g peptone, 3g potassium dihydrogen phosphate, 1.5g magnesium sulfate, and 1000ml water. The liquid bacteria are suspended with sterile water to prepare a concentration of 10%; S2, culturing in a constant temperature water bath at a temperature of 20°C-24°C-28°C-30°C for 3-8 days and 45-48 hours, and performing shake bottle fermentation on the golden ear liquid strain using a shaking device; S3, packing the treated Tremella fuciformis liquid strain into sterile test tubes, filtering them aseptically and sealing them; S4, complete the preparation of Tremella fuciformis liquid culture.

Images