In-situ sampler for microorganisms in water body

Abstract

The present invention discloses a water body microorganism in-situ sampler, and relates to the technical field of deep sea sampling, the water body microorganism in-situ sampler comprises: a device body, the lower end of which is provided with a sealed cabin; the controller is arranged in the sealed cabin body; the filter pump is arranged in the device body and electrically connected with the controller, and the device body is provided with a pipeline butt joint used for being communicated with the output end of the device body; the rotary mechanism is arranged in the device body, and a rotary disc is detachably arranged on the rotary mechanism; and the multi-layer filter membrane devices are uniformly distributed on the rotary disc along the circumferential direction, and the rotary mechanism drives the rotary disc to switch the multi-layer filter membrane devices connected with the pipeline butt joint. By arranging a plurality of multi-layer filter membrane devices, the sampling efficiency is effectively improved, and a plurality of high-quality microorganism samples can be obtained in one-time diving operation. By arranging the dismounting mechanism, the dismounting and mounting time of the filter disc consumables is effectively shortened, and the field operation efficiency is improved. The multilayer filter membrane device disclosed by the invention adopts a multilayer filter membrane structure, so that the filter area is enlarged, and the sampling amount is greatly improved.

Description

Technical Field

[0001] The invention relates to the technical field of deep sea sampling, and in particular to an in-situ sampler for water microorganisms. Background Art

[0002] Due to technical limitations, the deep sea is considered a difficult area for marine scientific research and ecological surveys. Deep-sea organisms have long adapted to the extreme environment of the deep sea, and are different from those in shallow seas in terms of species diversity, gene function, and ecological role, which urgently require in-depth research and development. However, the extreme environment of the deep sea, such as high pressure, low temperature, and darkness, makes the biological sampling process extremely challenging.

[0003] The traditional sampling method brings deep sea water to the shipboard laboratory for filtration. During the whole process, due to changes in temperature, salinity and hydrostatic pressure, coupled with a long experimental operation, the organisms will be physiologically damaged, resulting in the loss of in situ expression information. At present, there are related products on the market that can improve the quality of samples - the full-sea-depth microbial in-situ enrichment and fixation sampler ISMIFF (patent: a microbial in-situ automated enrichment and fixation device and method for full-sea depth), but the sampling efficiency is not high, the application range is limited, only one sample can be collected at a time, and the lack of continuous sampling ability cannot meet the research needs of continuous ecological monitoring and change laws.

[0004] The existing multi-sample sampling technology has some shortcomings. First, it is bulky and requires high operation on the mother ship or platform. Second, the operation is complicated and the efficiency is low. Third, the sampling volume of a single sample is low, which has a great impact on subsequent analysis. Summary of the invention

[0005] The purpose of the present invention is to address the deficiencies in the above-mentioned technologies and to propose an in-situ water microorganism sampler to solve the above-mentioned problems.

[0006] The present invention provides an in-situ water microorganism sampler, comprising: The device body has a sealed cabin at the lower end; A controller is arranged in the sealed cabin; The filter pump is arranged in the device body and is electrically connected to the controller. The device body is provided with a pipe joint for communicating with its output end; The rotating mechanism is arranged in the device body and has a rotating disk detachably arranged thereon; The multi-layer filter membrane device is evenly arranged on the rotating disk along the circumference. The rotating mechanism drives the rotating disk to switch the multi-layer filter membrane device connected to the pipeline joint, so that the filter disk in the multi-layer filter membrane device can switch multiple sequence sampling.

[0007] Preferably, the multi-layer filter membrane device also includes a filter disc cabin, a diverter pipe and a one-way valve. The filter disc cabin is fixedly arranged on the rotary disc, the filter disc is a multi-layer structure, and the multi-layer filter disc is arranged in the filter disc cabin at intervals along the vertical direction; the diverter pipe is fixedly arranged in the middle of the filter disc cabin, the one-way valve is fixedly arranged at the lower end of the filter disc cabin, the one-way valve is connected to the lower opening of the diverter pipe, and the one-way valve is connected to the pipeline joint through the rotation of the rotary disc, so that the seawater flowing into the one-way valve through the pipeline joint can flow to the filter disc in layers through the diverter pipe.

[0008] The inner circles of each layer of filter discs are vertically separated by gaskets, and the outer circles of each layer of filter discs are vertically separated by an annular member, and a plurality of liquid openings are circumferentially arranged on the annular member; the diverter pipe is provided with a plurality of circles of diverter holes from bottom to top, and the diverter holes are horizontally connected with the filter discs.

[0009] The filter disc includes two filter membranes spaced apart from each other, and a hollow plate disposed between the filter membranes. The diversion hole is located between the upper and lower filter membranes, so that seawater enters the hollow plate through the diversion hole and enters the filter cavity surrounded by the gasket and the annular member from the filter membrane.

[0010] A drainage cavity is arranged between the inner wall of the filter disc cabin and the outer wall of the multi-layer filter disc, and the filter cavity is connected to the drainage cavity through a liquid port. The filter disc cabin comprises an upper cabin and a lower cabin, and the upper cabin is connected to the lower cabin, and the connection between the upper cabin and the lower cabin is sealed by a sealing ring.

[0011] As another embodiment of the present invention, the rotating mechanism includes a driving motor and a turntable. The driving motor is disposed in the device body. The turntable is connected to the output end of the driving motor. The turntable is detachably connected to the turntable via a disassembly mechanism.

[0012] The disassembly mechanism includes a base block, a fixed sleeve, a clamping block, a lever, a pull rod and a spring. The base block is fixed on the turntable, the turntable is connected to the fixed sleeve, the clamping block and the lever are hinged to the inside and outside of the fixed sleeve respectively, one end of the pull rod is fixedly connected to the end of the clamping block, and the other end of the pull rod is slidably connected to the end of the pull rod; the spring is set on the pull rod and is located between the inner wall of the fixed sleeve and the clamping block; a limiting boss is provided on the base block, and a limiting groove engaged with the limiting boss is provided on the clamping block.

[0013] As another embodiment of the present invention, the sampler further includes an elastic pre-tightening mechanism, which is arranged on the device body and located on one side of the pipeline joint, and is rotatably connected to the rotating disk. The elastic pre-tightening mechanism includes a pressing sleeve rotatably sleeved on the outer ring of the rotating disk, and a limiting pressure rod vertically connected to the device body, and a spring 2 is sleeved on the upper end of the limiting pressure rod, and the spring 2 abuts against the pressing sleeve.

[0014] As another embodiment of the present invention, the sampler further includes an injection pump and a pressure gauge for filtering seawater, the pressure gauge is connected to the pipeline of the filter pump, and the injection pump is connected to the pipeline joint.

[0015] Compared with the prior art, it has the following beneficial effects: 1. The present invention effectively improves the sampling efficiency by providing multiple multi-layer filter membrane devices, thereby achieving the acquisition of multiple high-quality microbial samples in one diving operation.

[0016] 2. The present invention effectively shortens the time for disassembling and assembling filter disc consumables and improves on-site operation efficiency by providing a disassembly mechanism.

[0017] 3. The present invention provides a more convenient method for switching filter disc positions by setting a rotary mechanism, thereby simplifying the structure, reducing system complexity, and achieving higher efficiency.

[0018] 4. The multi-layer filter membrane device of the present invention adopts a multi-layer filter membrane structure to increase the filtration area and greatly increase the sampling volume. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the description of the embodiments will be briefly introduced below. Obviously, the drawings described below are only preferred embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0020] Figure 1 is a schematic diagram of the interior of the multi-layer filter membrane device of the present invention; Figure 2 It is a schematic plan view of an in-situ sampler for water microorganisms of the present invention; Figure 3 A top view of an in-situ water microorganism sampler according to the present invention; Figure 4 It is a schematic diagram of the connection between the multi-layer filter membrane device and the pipeline joint; Figure 5 is a schematic diagram of the disassembly mechanism of the present invention; Figure 6 Schematic diagram of the internal water flow direction of the multi-layer filter membrane device of the present invention.

[0021] In the figure, 1-device body; 2- Sealed cabin; 3-filter pump; 31-pipeline joint; 32-pressure gauge; 4- Rotating mechanism; 5-multi-layer filter membrane device; 51-filter disc cabin; 52-filter disc; 53-diverter pipe; 54-check valve; 55-gasket; 56-filter membrane cavity; 57-ring; 58-drainage cavity; 59-sealing ring; 521-filter membrane; 531-diverter hole; 571-liquid port; 6-turntable; 7-disassembly mechanism; 71-base block; 72-fixing sleeve; 73-block; 74-shift lever; 75-pull rod; 76-spring 1; 711-limiting boss; 731-limiting groove; 8-elastic preload mechanism; 81-pressing sleeve; 82-limiting pressure rod; 83-spring 2; 9-Syringe pump. DETAILED DESCRIPTION

[0022] In order to make it easier to understand the structure of the present invention and the functional features and advantages that can be achieved, the preferred embodiments of the present invention are described in detail with reference to the drawings as follows: Embodiment 1: like Figures 1 to 6 As shown, the present invention provides an in-situ water microorganism sampler, comprising: The device body 1 has a sealed cabin 2 at the lower end; A controller is arranged in the sealed cabin 2; The filter pump 3 is arranged in the device body 1 and is electrically connected to the controller. The device body 1 is provided with a pipe joint 31 for communicating with its output end; A rotating mechanism 4 is disposed in the device body 1 and has a detachable rotating disk 6 thereon; The multi-layer filter membrane device 5 is evenly arranged on the turntable 6 along the circumferential direction. The rotating mechanism 4 drives the turntable 6 to switch the multi-layer filter membrane device 5 connected to the pipeline joint 31, so that the filter disk 52 in the multi-layer filter membrane device 5 can switch the multi-sequence sampling to realize the sampling by switching the positions of multiple multi-layer filter membrane devices 5 on the turntable 6.

[0023] See also Figure 1 and Figure 6 The multi-layer filter membrane device 5 of the present invention further includes a filter disc cabin 51, a shunt pipe 53 and a one-way valve 54. The filter disc cabin 51 is fixedly arranged on the rotary disc 6. The filter disc 52 is a multi-layer structure. The multi-layer filter disc 52 is arranged in the filter disc cabin 51 at intervals along the vertical direction; the shunt pipe 53 is fixedly arranged in the middle of the filter disc cabin 51, and the one-way valve 54 is fixedly arranged at the lower end of the filter disc cabin 51. The one-way valve 54 is communicated with the lower opening of the shunt pipe 53. The one-way valve 54 is connected to the pipeline joint 31 through the rotation of the rotary disc 6, so that the seawater flowing into the one-way valve 54 through the pipeline joint 31 can flow to the filter disc 52 in layers through the shunt pipe 53.

[0024] See also Figure 1 In the present invention, the inner circles of each layer of filter discs 52 are vertically separated by gaskets 55, and the outer circles of each layer of filter discs 52 are vertically separated by an annular member 57. A plurality of liquid openings 571 are circumferentially arranged on the annular member 57; the diverter pipe 53 is provided with a plurality of circles of diverter holes 531 from bottom to top, and the diverter holes 531 are horizontally connected to the filter discs 52.

[0025] See also Figure 1 The filter disc 52 of the present invention includes two filter membranes 521 arranged at intervals in the upper and lower parts, and a hollow plate arranged between the filter membranes 521. The diverter hole 531 is located between the filter membranes 521 arranged in the upper and lower parts, and is connected to the filter membrane cavity 56 composed of the two filter membranes 521 arranged in the upper and lower parts, so that seawater enters the hollow plate through the diverter hole 531, and enters the filter cavity surrounded by the gasket 55 and the annular member 57 from the filter membrane 521. The multi-layer filter membrane device 5 adopts a multi-layer filter membrane 521 structure inside, has a unified filtering channel, can filter multiple layers of filter membranes 521 at the same time, and increases the filtering area. After filtering, the filter membrane 521 can be completely removed.

[0026] See also Figure 1 and Figure 6 , a drainage cavity 58 is provided between the inner wall of the filter disc cabin 51 and the outer wall of the multi-layer filter disc 52 of the present invention, and the filter cavity is connected to the drainage cavity 58 through a liquid port 571. The interior of the filter disc cabin 51 is designed with a multi-layer filter membrane 521, and the filter membrane 521 is installed inside the filter disc 52. The multi-layer filter membrane 521 structure can simultaneously load 6 layers of filter membranes 521, and the filter membranes 521 are separated by gaskets 55. The same method can increase the internal space and load more layers. The multi-layer filter membrane device 5 is designed with a multi-layer flow channel, and each layer of filter membrane 521 is simultaneously connected to the inlet of the filter disc cabin 51, and multi-layer filtering can be performed at the same time. The filtered liquid flows to the outlet of the drainage cavity 58 through the liquid port 571 for external discharge. The one-way valve 54 is used to prevent seawater from entering the filter disc 52 during the diving process of the equipment, resulting in the introduction of microorganisms from other water layers. After the sampling is completed, the filter disc 52 is disassembled, and the filter membranes 521 can be completely removed.

[0027] See also Figure 2 The rotary mechanism 4 of the present invention includes a driving motor and a turntable. The driving motor is arranged in the device body 1, and the turntable is connected to the output end of the driving motor. The turntable 6 is detachably connected to the turntable through the disassembly mechanism 7. The switching of multiple samples is mainly achieved through the rotary mechanism 4 and the pipeline joint 31. The rotary mechanism 4 has a precise angle control function, which drives the multi-layer filter membrane device 5 equipped with multiple filter discs 52 to rotate to achieve the switching of the filtering station. The inlet of the filter disc 52 rotates with the turntable 6 to automatically switch the liquid inlet and outlet to achieve the docking of different multi-layer filter membrane devices 5.

[0028] See also Figure 5The disassembly mechanism 7 of the present invention comprises a base block 71, a fixed sleeve 72, a clamping block 73, a lever 74, a pull rod 75 and a spring 76. The base block 71 is fixed on the turntable, the turntable 6 is connected to the fixed sleeve 72, the clamping block 73 and the lever 74 are respectively hinged to the inside and outside of the fixed sleeve 72, one end of the pull rod 75 is fixedly connected to the end of the clamping block 73, and the other end of the pull rod 75 is slidably connected to the end of the lever 74; the spring 76 is sleeved on the pull rod 75 and is located between the inner wall of the fixed sleeve 72 and the clamping block 73; the base block 71 is provided with a limiting boss 711, and the clamping block 73 is provided with a limiting groove 731 engaged with the limiting boss 711. The disassembly mechanism 7 is divided into two groups, and the two groups of disassembly mechanisms 7 are symmetrically arranged. During installation, the fixing sleeve 72 is inserted into the base block 71, and the clamping block 73 will be pushed open. After being inserted to the bottom, the clamping block 73 is installed in a limited position through the limiting groove 731; during removal, the lever 74 is pressed, the clamping block 73 rotates outward, disengages from the limiting boss 711 on the base block 71, and is pulled upward to complete the removal of the multi-layer filter membrane device 5.

[0029] Embodiment 2: like Figure 4 As shown, the present invention further includes an elastic pre-tightening mechanism 8, which is arranged on the device body 1 and located on one side of the pipeline joint 31, and the elastic pre-tightening mechanism 8 is rotatably connected to the rotating disk 6. The elastic pre-tightening mechanism 8 includes a pressing sleeve 81 rotatably sleeved on the outer ring of the rotating disk 6, and a limiting pressure rod 82 vertically connected to the device body 1, and a spring 2 83 is sleeved on the upper end of the limiting pressure rod 82, and the spring 2 83 abuts against the pressing sleeve 81 to ensure the pressing force between the pipeline joint 31 and the rotating disk 6.

[0030] Embodiment three: like Figure 2 As shown, the present invention also includes an injection pump 9 and a pressure gauge 32 for filtering seawater, the pressure gauge 32 is connected to the pipeline of the filter pump 3, and the injection pump 9 is connected to the pipeline joint 31. The edge design of the pipeline joint 31 introduces chamfering to facilitate the rotation and guidance of the filter disc 52. At the same time, the circle on the end face of the pipeline joint 31 is sealed with the water inlet below the filter disc 52. The way of obtaining samples is to enrich the microorganisms in the seawater on a filter membrane 521 cabin by filtering a large amount of seawater. After the filtration is completed, the buffer solution / fixative solution is accurately injected into the filter disc 52 by the injection pump 9 to wrap the filter membrane 521, and the in-situ fixed sampling of the microorganisms is completed. During the filtration process, the pressure change in the pipeline is monitored by the pressure gauge 32, and the real-time acquisition is performed by the controller, and the flow rate of the filter pump 3 is adjusted, and the internal pressure of the pipeline can also be adjusted.

[0031] Working principle of the present invention: 1. Cleaning pipeline: The filter pump 3 is running to clean the pipeline with seawater; 2. Workstation transfer: The rotary mechanism 4 operates to transfer the filter disc 52 to be filtered to the working station; 3. Docking of the filter disc 52: The pipe docking joint 31 automatically aligns with the inlet of the filter disc 52 to complete the docking of the filter disc 52 and the filter pipe; 4. Seawater filtration: The pump rotates, and the microorganisms are enriched and filtered through the filter disc 52 and the multi-layer filter membrane 521 structure; 5. In-situ fixation: The syringe works to inject the set buffer solution / fixative solution into the filter membrane cavity 56 to fix the microorganisms in situ, thus completing one sampling of one station; 6. Cleaning the pipeline: the rotary mechanism 4 is operated to disconnect the pipeline interface from the inlet of the filter disc 52, and the filter pump 3 is operated to clean the pipeline with seawater; 7. Switch to the next workstation: The rotary mechanism 4 operates and transfers to the next workstation, repeating steps 2 to 6 to complete the enrichment and fixation of a single filter disc 52 until the enrichment and fixation of microorganisms on all filter discs 52 are completed.

[0032] The above are only preferred embodiments of the present invention, and do not limit the present invention in any form. Any technician familiar with the art can make many possible changes and modifications to the technical solution of the present invention by using the above technical content without departing from the scope of the technical solution of the present invention, or modify it into an equivalent embodiment of equivalent changes. Therefore, any changes, modifications, equivalent changes and modifications made to the above embodiments based on the technology of the present invention without departing from the content of the technical solution of the present invention belong to the protection scope of the present technical solution.

Claims

1. A water microorganism in-situ sampler, characterized in that: include: The device body (1) has a sealed cabin (2) at the lower end; A controller, arranged in the sealed cabin (2); A filter pump (3) is disposed in the device body (1) and is electrically connected to the controller; the device body (1) is provided with a pipe joint (31) for communicating with its output end; A rotating mechanism (4) is arranged in the device body (1), and a rotating disk (6) is detachably provided on the rotating mechanism (4); The multi-layer filter membrane device (5) is evenly arranged on the rotating disk (6) along the circumferential direction, and the rotating mechanism (4) drives the rotating disk (6) to switch the multi-layer filter membrane device (5) connected to the pipeline joint (31), thereby switching the filter disk (52) in the multi-layer filter membrane device (5) for multi-sequence sampling; The multi-layer filter membrane device (5) further comprises a filter disc cabin (51), a diverter pipe (53) and a one-way valve (54); the filter disc cabin (51) is fixedly arranged on the rotary disc (6); the filter disc (52) is a multi-layer structure; the filter discs (52) of the multi-layer structure are arranged in the filter disc cabin (51) at intervals in the vertical direction; the diverter pipe (53) is fixedly arranged in the middle of the filter disc cabin (51); the one-way valve (54) is fixedly arranged at the lower end of the filter disc cabin (51); the one-way valve (54) is communicated with the lower opening of the diverter pipe (53); the one-way valve (54) is connected to the pipeline joint (31) through the rotation of the rotary disc (6), so that the seawater flowing into the one-way valve (54) through the pipeline joint (31) flows into the filter disc (52) in layers through the diverter pipe (53); The inner circles of each layer of the filter discs (52) are vertically separated by gaskets (55), and the outer circles of each layer of the filter discs (52) are vertically separated by an annular member (57), and a plurality of liquid through openings (571) are provided on the annular member (57) along the circumference; the flow diversion pipe (53) is provided with a plurality of circles of flow diversion holes (531) from bottom to top, and the flow diversion holes (531) are horizontally connected to the filter discs (52).

2. The water body microorganism in-situ sampler according to claim 1, characterized in that: The filter disc (52) comprises two filter membranes (521) spaced apart from each other, and a hollow plate disposed between the filter membranes (521); the diversion hole (531) is located between the filter membranes (521) disposed above and below, so that seawater can enter the hollow plate through the diversion hole (531) and enter the filter cavity surrounded by the gasket (55) and the annular member (57) from the filter membrane (521).

3. The water body microorganism in-situ sampler according to claim 2, characterized in that: A drainage cavity (58) is provided between the inner wall of the filter disc chamber (51) and the outer wall of the multi-layer filter disc (52), and the filter cavity is in communication with the drainage cavity (58) via the liquid port (571).

4. The water body microorganism in-situ sampler according to claim 1, characterized in that: The rotating mechanism (4) comprises a driving motor and a rotating disk, the driving motor being arranged in the device body (1), the rotating disk being connected to the output end of the driving motor, and the rotating disk (6) being detachably connected to the rotating disk via a disassembly mechanism (7).

5. The water body microorganism in-situ sampler according to claim 4, characterized in that: The disassembly mechanism (7) comprises a base block (71), a fixed sleeve (72), a clamping block (73), a lever (74), a pull rod (75) and a spring (76); the base block (71) is fixed on the turntable; the turntable (6) is connected to the fixed sleeve (72); the clamping block (73) and the lever (74) are respectively hinged to the inside and outside of the fixed sleeve (72); one end of the lever (75) is fixedly connected to the end of the clamping block (73); the other end of the lever (75) is slidably connected to the end of the lever (74); the spring (76) is sleeved on the lever (75) and is located between the inner wall of the fixed sleeve (72) and the clamping block (73); a limiting boss (711) is provided on the base block (71); and a limiting groove (731) is provided on the clamping block (73) to be clamped with the limiting boss (711).

6. The water body microorganism in-situ sampler according to claim 4, characterized in that: It also includes an elastic pre-tightening mechanism (8), which is arranged on the device body (1) and located on one side of the pipeline joint (31), and the elastic pre-tightening mechanism (8) is rotatably connected to the rotating disk (6).

7. The water body microorganism in-situ sampler according to claim 6, characterized in that: The elastic preload mechanism (8) comprises a pressing sleeve (81) rotatably sleeved on the outer ring of the rotating disk (6), and a limiting pressure rod (82) vertically connected to the device body (1), wherein a second spring (83) is sleeved on the upper end of the limiting pressure rod (82), and the second spring (83) abuts against the pressing sleeve (81).

8. The water body microorganism in-situ sampler according to claim 1, characterized in that: It also comprises an injection pump (9) and a pressure gauge (32) for filtering seawater, wherein the pressure gauge (32) is connected to the pipeline of the filtering pump (3), and the injection pump (9) is connected to the pipeline joint (31).

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