Multifunctional microorganism automatic inspection equipment

Abstract

The invention belongs to the technical field of microbiological inspection, and particularly relates to multifunctional microbiological automatic inspection equipment which comprises a liquid injection dilution tank, an inner cavity is formed in the bottom of the liquid injection dilution tank, the side face of the liquid injection dilution tank is fixedly connected with a split-flow liquid drainage tank, and the side face of the split-flow liquid drainage tank is fixedly connected with an automatic inspection tank. The side surface of the automatic inspection box is fixedly connected with a detection feedback box, and a plurality of groups of limiting plates drive test tubes to rotate, so that the interference of bubbles on experimental results can be reduced, the accuracy and reliability of the experimental results can be guaranteed, the influence of the bubbles on subsequent procedures can be reduced, and the inspection working efficiency can be improved. Secondly, the bottom of the baffle plate is attached to the top of the test tube, so that sample liquid in the test tube can be prevented from splashing when the test tube rotates, damage, such as corrosion and blockage, of experimental equipment caused by liquid splashing is avoided, the maintenance and cleaning frequency of the equipment can be reduced, and the cost of microbiological inspection can be reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of microbiological testing, in particular to a multifunctional automatic microbiological testing device. Background Art

[0002] The multifunctional microbial automatic testing equipment is an automated instrument that integrates multiple functional modules such as transmission clamping, liquid injection and dilution, shunt discharge and automated testing. It can automatically complete the steps of clamping microbial culture dishes, bacterial liquid dilution, shunt discharge and automated liquid extraction and counting of secondary dilution liquid, thereby achieving rapid and accurate testing of microorganisms.

[0003] In the existing technology, electric guide rails are used to accurately move samples and reagents through the transmission and clamping function; and the diluent is quantitatively injected through a precision pump system to automatically complete the dilution of the sample. Secondly, the flow direction and discharge volume of the liquid are controlled to achieve accurate distribution of samples and reagents. At the same time, optical, electrochemical and other sensors are used to monitor the growth and metabolic changes of microorganisms in real time. Finally, the samples are received, pretreated, inoculated and cultured to ensure the applicability of the samples.

[0004] There are still some problems in the actual application of the above scheme. When the test project does not require high uniformity of the solution, it is not necessary to stir the solution. At this time, when the sample in the test tube at the same height is diluted, the liquids of different concentrations will be mixed. Due to the existence of the concentration gradient, the ability to dissolve gas will change, thereby generating bubbles, and the bubbles will occupy a certain space. In spectral detection and chromatographic detection, bubbles will change the scattering or absorption of light, affecting detection signals such as absorbance, resulting in inaccurate test results. At the same time, in order to avoid interference from bubbles, sample processing, inoculation, and cultivation operations will become more difficult, which will reduce the efficiency of the inspection work. Secondly, when debubbling solutions with lower viscosity, the gas encounters less resistance during the rising process and the speed is relatively large, which will cause liquid splashing. The splashed solution will adhere to the internal components of the equipment, causing corrosion, wear or blockage of the components, thereby requiring more frequent equipment cleaning and maintenance, which will increase the maintenance cost and time of the equipment, and thus increase the cost of microbial testing.

[0005] To this end, the present invention provides a multifunctional automatic microbial testing device. Summary of the invention

[0006] In order to make up for the deficiencies of the prior art, at least one technical problem raised in the background technology is solved.

[0007] The technical solution adopted by the present invention to solve the technical problem is as follows: the multifunctional microorganism automatic testing equipment described in the present invention comprises a liquid injection and dilution box, the bottom of which is provided with an inner cavity, the side of which is fixedly connected to a diversion and drainage box, the side of which is fixedly connected to an automated testing box, and the side of which is fixedly connected to a detection feedback box;

[0008] The injection and dilution box is used to dilute the solution, the diversion and drainage box distributes the solution in the test tube to different test tubes through the diversion mechanism, and discharges the excess liquid through the drainage mechanism, the automated inspection box detects the solution after the diversion is completed, and the detection feedback box performs real-time detection based on the data transmitted by the automated inspection box;

[0009] A transmission dilution assembly is provided at the top of the inner cavity of the liquid injection and dilution box, and the transmission dilution assembly includes an electric guide rail fixedly connected to the top of the inner cavity of the liquid injection and dilution box, a sliding cylinder is slidably connected inside the electric guide rail, and a pneumatic gripper is fixedly connected to the output shaft of the sliding cylinder, a fixed plate is fixedly connected to the bottom of the inner cavity of the liquid injection and dilution box, an electric telescopic rod is fixedly connected to the top of the fixed plate, and a dilution head is fixedly connected to the output end of the electric telescopic rod, and a rotating defoaming mechanism is provided in the inner cavity at the bottom of the liquid injection and dilution box;

[0010] The rotating defoaming mechanism includes a forward and reverse motor fixedly arranged at the bottom of the inner cavity of the liquid injection and dilution box, a rotating disc is fixedly arranged on the output shaft of the forward and reverse motor, and a plurality of groups of limiting plates are slidably arranged on the upper part of the rotating disc. The test tube can be limited and rotated by the aggregation of the plurality of groups of limiting plates.

[0011] Preferably, a connecting rod is fixedly connected to the upper part of the rotating disc, one end of the connecting rod is fixedly connected to a rotating base plate, a polygonal groove is opened inside the rotating base plate, a displacement plate is slidably connected inside the polygonal groove, the top of the displacement plate is fixedly connected to a limiting plate, and the top of the limiting plate is fixedly connected to a limiting column.

[0012] Preferably, the rotation of the rotating base plate will drive the displacement plate, the limit plate and the limit column to move synchronously, and the movement of the rotating base plate can provide power for subsequent equipment.

[0013] Preferably, the outer ring surface of the rotating bottom plate is sleeved with a rotating outer shell, a guide groove is penetrated through the upper part of the rotating outer shell, an arc-shaped opening is opened on the outer ring surface of the rotating outer shell, and the connecting rod is slidably connected inside the arc-shaped opening.

[0014] Preferably, the guide grooves are provided to achieve the purpose of aggregation and diffusion through the limiting of the limiting pillars while the limiting plate moves, thereby limiting the outer annular surface of the test tube.

[0015] Preferably, a first oblique block is fixedly connected to the outer ring surface of the rotating shell, the first oblique block and the arc-shaped opening are symmetrically distributed about the central axis of the rotating shell, a return spring is fixedly connected to the inner cavity side of the liquid injection and dilution box, and one end of the return spring is fixedly connected to the second oblique block.

[0016] Preferably, the arrangement of the first oblique block and the second oblique block can keep the rotating shell from rotating before the limiting plate is aggregated, and the arrangement of the reset spring will shrink when the side wall of the limiting plate fits with the outer annular surface of the test tube, thereby driving the test tube to rotate synchronously, thereby reducing the bubbles inside the solution and improving the accuracy of sample detection.

[0017] Preferably, a limiting shielding mechanism for shielding is provided at the bottom of the inner cavity of the liquid injection and dilution box, and the limiting shielding mechanism includes an external motor, and the external motor is fixedly connected to the side wall of the fixed plate, and the output shaft of the external motor is fixedly connected to a rotating screw, and the outer ring surface of the rotating screw is threadedly connected to a reciprocating cylinder, and the outer ring surface of the reciprocating cylinder is provided with a limiting ring, and the reciprocating cylinder is slidably connected in the limiting ring, and the rotating screw passes through and is rotatably connected to the inside of the fixed plate, and one end of the limiting ring is fixedly connected to the side of the fixed plate.

[0018] Preferably, one end of the reciprocating cylinder is fixedly connected to a limiting semi-ring, a groove is provided on the inner arc surface of the limiting semi-ring, a rotating column is rotatably connected to the side wall of the groove on the inner arc surface of the limiting semi-ring, an L-shaped plate is fixedly connected to the upper part of the limiting semi-ring, the L-shaped plate is composed of a vertical plate and a horizontal plate, an inner cavity is provided inside the horizontal plate of the L-shaped plate, a reciprocating spring is fixedly connected to the side wall of the inner cavity of the L-shaped plate, and a baffle plate is fixedly connected to one end of the reciprocating spring.

[0019] Preferably, the movement of the reciprocating cylinder can limit the middle of the test tube through the limiting semi-ring without hindering the operation of the transmission and dilution assembly, the rotating column can provide support for the rotation of the test tube, and the contraction of the baffle plate can block the mouth of the test tube, thereby avoiding sample overflow and reducing the cost of microbial testing.

[0020] The beneficial effects of the present invention are as follows:

[0021] 1. A multifunctional microorganism automatic testing device described in the present invention, when the rotating base plate rotates, since the side surfaces of the first oblique block and the second oblique block are in a fitted state, and since the side surfaces of the multiple sets of limiting plates are not blocked, when the rotating base plate rotates at this time, the limiting posts of the guide grooves will be used to limit the multiple sets of limiting plates so that they will be synchronously aggregated inwards, and when the side surfaces of the multiple sets of limiting plates are in fit with the outer annular surface of the test tube, since the output of the forward and reverse motors will continue to rotate in one direction and the inward aggregation of the multiple sets of limiting plates will be blocked by the outer annular surface of the test tube, the rotating outer annular surface will be driven by the limiting posts and the guide grooves. The shell rotates synchronously. When the rotating shell rotates, it will drive the first oblique block fixed on the outer ring surface of the rotating shell to rotate synchronously, and push the second oblique block back to the inner cavity at the bottom of the injection dilution box through its oblique structure. At this time, the reset spring is in a contracted state, so the rotating shell rotates synchronously with the rotating bottom plate, and drives the test tube to rotate through multiple sets of limit plates during rotation, thereby reducing the interference of bubbles on the experimental results, ensuring the accuracy and reliability of the experimental results, and secondly reducing the influence of bubbles on subsequent processes, thereby improving the work efficiency of inspection.

[0022] 2. The multifunctional automatic microbial testing equipment described in the present invention, when the reciprocating cylinder performs linear motion, it will drive the limiting semi-ring to perform synchronous motion, when the rotating column on the inner arc surface of the limiting semi-ring fits with the outer ring surface in the middle of the test tube, the external motor will stop moving, but when the limiting semi-ring moves, it will drive the L-shaped plate fixed on its upper part to perform synchronous motion, because the reciprocating spring is fixed on the side wall of the inner cavity opened inside the transverse plate of the L-shaped plate, so when the two groups of baffles are against each other, they will move in the direction of the reciprocating spring, and the reciprocating spring will be in a compressed state at this time, and the two groups of baffles will be against each other, and the bottom will fit with the top of the test tube, and the bottom of the baffle will fit with the top of the test tube, so that the internal sample liquid of the test tube can be prevented from splashing when the test tube rotates, thereby avoiding damage to the experimental equipment caused by liquid splashing, such as corrosion, blockage, etc., thereby reducing the frequency of equipment maintenance and cleaning, thereby reducing the cost of microbial testing. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The present invention will be further described below in conjunction with the accompanying drawings.

[0024] Figure 1 It is a schematic diagram of the overall structure of a preferred embodiment of the present invention;

[0025] Figure 2 It is a schematic diagram of the cross-sectional structure of the liquid injection and dilution box shown in the present invention;

[0026] Figure 3 The present invention shows Figure 2 The enlarged structural diagram at A in the middle;

[0027] Figure 4It is a schematic diagram of the three-dimensional structure of the rotating defoaming mechanism shown in the present invention;

[0028] Figure 5 It is a schematic diagram of the exploded structure of some components of the rotating defoaming mechanism shown in the present invention;

[0029] Figure 6 It is a schematic diagram of the position structure of the rotating defoaming mechanism, the limiting shielding mechanism and the transmission dilution component shown in the present invention;

[0030] Figure 7 It is a schematic diagram of the three-dimensional structure of the position-limiting and shielding mechanism shown in the present invention;

[0031] Figure 8 It is a schematic diagram of the internal structure of the reciprocating cylinder shown in the present invention;

[0032] Fig. 9 It is a schematic diagram of the internal structure of the L-shaped plate shown in the present invention;

[0033] In the figure: 1. Liquid injection and dilution box; 2. Diversion and drainage box; 3. Automated inspection box; 4. Detection feedback box;

[0034] 5. Rotating defoaming mechanism; 501. Forward and reverse motor; 502. Rotating disc; 503. Connecting rod; 504. Rotating bottom plate; 505. Polygonal trough; 506. Displacement plate; 507. Limiting plate; 508. Limiting column; 509. Rotating housing; 510. Guide groove; 511. First oblique block; 512. Arc-shaped opening; 513. Return spring; 514. Second oblique block;

[0035] 6. Position-limiting shielding mechanism; 601. fixing plate; 602. external motor; 603. rotating screw; 604. reciprocating cylinder; 605. position-limiting ring; 606. position-limiting semi-ring; 607. rotating column; 608. L-shaped plate; 609. reciprocating spring; 610. shielding plate;

[0036] 7. Transmission and dilution assembly; 701. Electric guide rail; 702. Sliding cylinder; 703. Pneumatic gripper; 704. Electric telescopic rod; 705. Dilution head. DETAILED DESCRIPTION

[0037] In order to make the technical means, creative features, objectives and effects achieved by the present invention easy to understand, the present invention is further explained below in conjunction with specific implementation methods.

[0038] Embodiment 1

[0039] like Figures 1 to 9As shown, a multifunctional microorganism automatic testing device according to an embodiment of the present invention comprises a liquid injection and dilution box 1, wherein an inner cavity is provided at the bottom of the liquid injection and dilution box 1, a shunt and drainage box 2 is fixedly connected to the side of the liquid injection and dilution box 1, an automated testing box 3 is fixedly connected to the side of the shunt and drainage box 2, and a detection feedback box 4 is fixedly connected to the side of the automated testing box 3;

[0040] The injection and dilution box 1 is used to dilute the solution, the diversion and drainage box 2 distributes the solution in the test tube to different test tubes through the diversion mechanism, and discharges the excess liquid through the drainage mechanism, the automated inspection box 3 detects the solution after the diversion is completed, and the detection feedback box 4 performs real-time detection based on the data transmitted by the automated inspection box 3;

[0041] A transmission dilution component 7 is provided at the top of the inner cavity of the liquid injection and dilution box 1, and the transmission dilution component 7 includes an electric guide rail 701 fixedly connected to the top of the inner cavity of the liquid injection and dilution box 1, a sliding cylinder 702 is slidably connected inside the electric guide rail 701, and a pneumatic clamp 703 is fixedly connected to the output shaft of the sliding cylinder 702, a fixed plate 601 is fixedly connected to the bottom of the inner cavity of the liquid injection and dilution box 1, an electric telescopic rod 704 is fixedly connected to the top of the fixed plate 601, and a dilution head 705 is fixedly connected to the output end of the electric telescopic rod 704, and a rotating defoaming mechanism 5 is provided in the inner cavity at the bottom of the liquid injection and dilution box 1;

[0042] The rotating debubbling mechanism 5 includes a forward and reverse motor 501 fixedly arranged at the bottom of the inner cavity of the liquid injection and dilution box 1, and a rotating disc 502 is fixedly arranged on the output shaft of the forward and reverse motor 501. A plurality of groups of limiting plates 507 are slidably arranged on the upper part of the rotating disc 502. The test tube can be limited and rotated by the aggregation of the plurality of groups of limiting plates 507, so that the bubbles inside the solution can be removed, thereby improving the accuracy of the test results.

[0043] Specifically, when the test item does not require high uniformity of the solution, it is not necessary to stir the solution. At this time, when the sample in the test tube is diluted, liquids of different concentrations will be mixed. Due to the existence of the concentration gradient, the ability to dissolve gas will change, thereby generating bubbles, and the bubbles will occupy a certain space. In spectral detection and chromatographic detection, bubbles will change the scattering or absorption of light, affecting the detection signals such as absorbance, resulting in inaccurate test results. At the same time, in order to avoid the interference of bubbles, sample processing, inoculation, culturing and other operations will become more difficult, which will reduce the efficiency of the inspection work;

[0044] Therefore, the present invention solves this problem by setting a corresponding structure. In the multifunctional microbial automatic testing equipment described in the present invention, when it is necessary to test the solution, the test tube containing the solution is manually placed in the liquid injection dilution box 1. At this time, the dilution head 705 is driven to the top of the test tube mouth by the electric telescopic rod 704 on the top of the fixed plate 601, and the solution in the test tube is diluted. When the dilution is completed, the electric guide rail 701 is started to drive the sliding cylinder 702 and the pneumatic gripper 703 to move above the test tube, and the sliding cylinder 702 is started, so that the output shaft of the sliding cylinder 702 drives the pneumatic gripper 703 to descend. When the pneumatic gripper 70 When the pneumatic gripper 703 moves to the top of the test tube, the diluted solution is clamped by starting the pneumatic gripper 703, and the test tube is transported to the diversion and drainage box 2 through the electric guide rail 701. When the pneumatic gripper 703 drives the test tube to the diversion device, the diversion device will place the test tube in the correct position of the diverter through the positioning system. At this time, the valve in the diverter is started. At this time, the solution in the test tube will be guided to different test tubes through different pipes, and the excess liquid will be transported to the waste liquid collection system through the drainage mechanism. After the diversion work is completed, the test tubes are transported to the automated inspection box 3 respectively through the pneumatic gripper 703, and are transported through the diversion and drainage while being transported. The liquid box 2 synchronously transmits the relevant information of the solution to the automated test box 3. When the automated test box 3 completes the reception of the sample solution, it will accurately test and analyze the sample solution through its internal detection instruments and sensors, and transmit the results of the detection process to the detection feedback box 4 in real time. At this time, the detection feedback box 4 will interpret and judge the detection results and generate a corresponding detection report. However, when the detection project does not require high uniformity of the solution, it is not necessary to stir the solution. At this time, when the sample in the test tube is diluted, liquids of different concentrations will be mixed. Due to the existence of the concentration gradient, the ability to dissolve gas will change. Changes will occur, thus generating bubbles, which will occupy a certain space. In spectral detection and chromatographic detection, bubbles will cause changes in the scattering or absorption of light, affecting detection signals such as absorbance, resulting in inaccurate detection results. At the same time, in order to avoid the interference of bubbles, sample processing, inoculation, cultivation and other operations will become more difficult, which will reduce the efficiency of the inspection work. At this time, the starting of the forward and reverse motor 501 can drive the rotating disk 502 to rotate synchronously, and while rotating, the test tube can be limited and rotated through the aggregation of multiple groups of limiting plates 507, so that the bubbles inside the solution can be removed, thereby improving the accuracy of the test results.

[0045] Embodiment 2

[0046] like Figures 2 to 9 As shown in Comparative Example 1, another embodiment of the present invention is:

[0047] like Figure 4 and Figure 5As shown, the upper part of the rotating disk 502 described in this embodiment is fixedly connected to a connecting rod 503, one end of the connecting rod 503 is fixedly connected to a rotating base plate 504, a polygonal groove 505 is opened inside the rotating base plate 504, a displacement plate 506 is slidably connected inside the polygonal groove 505, the top of the displacement plate 506 is fixedly connected to a limiting plate 507, and the top of the limiting plate 507 is fixedly connected to a limiting column 508.

[0048] Specifically, after the solution is diluted, the forward and reverse motor 501 is started. At this time, the output shaft of the forward and reverse motor 501 will drive the rotating disk 502 to rotate synchronously. Since the rotating disk 502 and the rotating base plate 504 are rotating and are fixed to the upper part of the rotating disk 502 by the connecting rod 503, when the rotating disk 502 rotates, it will also drive the rotating base plate 504 to rotate, and at the same time drive the polygonal slot body 505 to move synchronously. Since the displacement plate 506 slides inside the polygonal slot body 505, when the rotating base plate 504 rotates, it will drive the displacement plate 506, the limit plate 507 and the limit column 508 to rotate synchronously, and the connecting rod 503 can provide corresponding power support for the operation of the subsequent device.

[0049] like Figure 4 As shown, the outer ring surface of the rotating base plate 504 in this embodiment is provided with a rotating shell 509, the upper part of the rotating shell 509 is penetrated by a guide groove 510, the outer ring surface of the rotating shell 509 is provided with an arc-shaped opening 512, and the connecting rod 503 is slidably connected inside the arc-shaped opening 512.

[0050] like Figure 3 As shown, in this embodiment, the outer ring surface of the rotating shell 509 is fixedly connected with a first oblique block 511, and the first oblique block 511 and the arc-shaped opening 512 are symmetrically distributed about the central axis of the rotating shell 509. The inner cavity side of the liquid injection and dilution box 1 is fixedly connected with a return spring 513, and one end of the return spring 513 is fixedly connected with a second oblique block 514.

[0051] Specifically, when the rotating base plate 504 rotates, since the side surfaces of the first oblique block 511 and the second oblique block 514 are in a fitted state, and since the side surfaces of the plurality of limit plates 507 are not blocked, when the rotating base plate 504 rotates, the limit posts 508 are limited by the guide grooves 510, so that the plurality of limit plates 507 are synchronously aggregated inwardly, and when the side surfaces of the plurality of limit plates 507 are in a fit with the outer annular surface of the test tube, since the output of the forward and reverse motor 501 will continue to rotate in one direction and the plurality of limit plates 507 are blocked by the outer annular surface of the test tube when they aggregate inwardly, the rotating housing 509 will be driven by the limit posts 508 and the guide grooves 510. Synchronous rotation, when the rotating shell 509 rotates, it will drive the first oblique block 511 fixed on the outer ring surface of the rotating shell 509 to rotate synchronously, and push the second oblique block 514 back to the inner cavity at the bottom of the injection and dilution box 1 through its oblique structure. At this time, the reset spring 513 is in a contracted state, so the rotating shell 509 rotates synchronously with the rotating bottom plate 504, and drives the test tube to rotate through multiple sets of limit plates 507 during rotation, thereby reducing the interference of bubbles on the experimental results, ensuring the accuracy and reliability of the experimental results, and secondly reducing the influence of bubbles on subsequent processes, thereby improving the work efficiency of inspection.

[0052] like Figure 7 and Figure 8 As shown, the bottom of the inner cavity of the liquid injection and dilution box 1 described in this embodiment is provided with a limiting shielding mechanism 6 for shielding, and the limiting shielding mechanism 6 includes an external motor 602, and the external motor 602 is fixedly connected to the side wall of the fixed plate 601, and the output shaft of the external motor 602 is fixedly connected to a rotating screw 603, and the outer ring surface of the rotating screw 603 is threadedly connected to a reciprocating cylinder 604, and the outer ring surface of the reciprocating cylinder 604 is provided with a limiting ring 605, and the reciprocating cylinder 604 is slidably connected in the limiting ring 605, and the rotating screw 603 passes through and is rotatably connected to the inside of the fixed plate 601, and one end of the limiting ring 605 is fixedly connected to the side of the fixed plate 601.

[0053] Specifically, when the rotating defoaming mechanism 5 is started, the external motor 602 is started at the same time. At this time, the output shaft of the external motor 602 will drive the rotating screw 603 to rotate. Since the rotating screw 603 is threadedly connected to the reciprocating cylinder 604, when the rotating screw 603 rotates, it will synchronously drive the reciprocating cylinder 604 to rotate. However, since the circular ring on the side of the limiting ring 605 away from the fixed plate 601 is provided with a groove that is compatible with the size of the protrusion on the outer ring surface of the reciprocating cylinder 604, when the rotating screw 603 rotates, the reciprocating cylinder 604 can move in a straight line through the cooperation of the groove and the protrusion.

[0054] like Figure 8 and Fig. 9As shown, in this embodiment, one end of the reciprocating cylinder 604 is fixedly connected to a limiting semi-ring 606, the inner arc surface of the limiting semi-ring 606 is provided with a groove, and the side wall of the groove on the inner arc surface of the limiting semi-ring 606 is rotatably connected to a rotating column 607, and the upper part of the limiting semi-ring 606 is fixedly connected to an L-shaped plate 608, and the L-shaped plate 608 is composed of a vertical plate and a transverse plate, and the transverse plate of the L-shaped plate 608 has an inner cavity, and the side wall of the inner cavity of the L-shaped plate 608 is fixedly connected to a reciprocating spring 609, and one end of the reciprocating spring 609 is fixedly connected to a shielding plate 610.

[0055] Specifically, when the reciprocating cylinder 604 performs linear motion, it will drive the limiting semi-ring 606 to perform synchronous motion. When the rotating column 607 on the inner arc surface of the limiting semi-ring 606 fits with the outer ring surface in the middle of the test tube, the external motor 602 will stop moving. However, when the limiting semi-ring 606 moves, it will drive the L-shaped plate 608 fixed on its upper part to perform synchronous motion. Since the reciprocating spring 609 is fixed on the side wall of the inner cavity opened inside the transverse plate of the L-shaped plate 608, when the two sets of baffles 610 are against each other, they will move in the direction of the reciprocating spring 609. At this time, the reciprocating spring 609 is in a compressed state, and the two sets of baffles 610 will be against each other, and the bottom will fit with the top of the test tube. By having the bottom of the baffle 610 fit with the top of the test tube, the sample liquid inside the test tube can be prevented from splashing when the test tube rotates, thereby avoiding damage to the experimental equipment caused by liquid splashing, such as corrosion, blockage, etc., thereby reducing the frequency of equipment maintenance and cleaning, thereby reducing the cost of microbial testing.

[0056] Working principle: after the solution is diluted, start the forward and reverse motor 501. At this time, the output shaft of the forward and reverse motor 501 will drive the rotating disk 502 to rotate synchronously. Since the rotating disk 502 and the rotating base plate 504 are rotating and fixed to the upper part of the rotating disk 502 by the connecting rod 503, when the rotating disk 502 rotates, it will also drive the rotating base plate 504 to rotate, and at the same time drive the polygonal slot body 505 to move synchronously. Since the displacement plate 506 slides inside the polygonal slot body 505, when the rotating base plate 504 rotates, it will drive the displacement plate 506, the limit plate 507 and the limit column 508 to rotate synchronously, and the connecting rod 503 can provide corresponding power support for the operation of the subsequent device.

[0057] When the rotating base plate 504 rotates, since the side surfaces of the first oblique block 511 and the second oblique block 514 are in a fitted state, and since the side surfaces of the multiple sets of limiting plates 507 are not blocked, when the rotating base plate 504 rotates, the limiting columns 508 are limited by the guide grooves 510, so that the multiple sets of limiting plates 507 are synchronously aggregated inwardly, and when the side surfaces of the multiple sets of limiting plates 507 are in a fit with the outer annular surface of the test tube, since the output of the forward and reverse motor 501 will continue to rotate in one direction and the multiple sets of limiting plates 507 are blocked by the outer annular surface of the test tube, the rotating housing 509 will be driven to rotate synchronously through the limiting columns 508 and the guide grooves 510. Rotation, when the rotating shell 509 rotates, it will drive the first oblique block 511 fixed on the outer ring surface of the rotating shell 509 to rotate synchronously, and push the second oblique block 514 back to the inner cavity at the bottom of the injection and dilution box 1 through its oblique structure. At this time, the reset spring 513 is in a contracted state, so the rotating shell 509 rotates synchronously with the rotating bottom plate 504, and drives the test tube to rotate through multiple sets of limit plates 507 during rotation, thereby reducing the interference of bubbles on the experimental results, ensuring the accuracy and reliability of the experimental results, and secondly reducing the influence of bubbles on subsequent processes, thereby improving the work efficiency of inspection.

[0058] When the rotating defoaming mechanism 5 is started, the external motor 602 is started. At this time, the output shaft of the external motor 602 will drive the rotating screw 603 to rotate. Since the rotating screw 603 is threadedly connected to the reciprocating cylinder 604, when the rotating screw 603 rotates, it will synchronously drive the reciprocating cylinder 604 to rotate. However, since the circular ring on the side of the limit ring 605 away from the fixed plate 601 is provided with a groove that is compatible with the size of the protrusion on the outer ring surface of the reciprocating cylinder 604, when the rotating screw 603 rotates, the reciprocating cylinder 604 can move in a straight line through the cooperation of the groove and the protrusion.

[0059] When the reciprocating cylinder 604 performs linear motion, it will drive the limiting semi-ring 606 to perform synchronous motion. When the rotating column 607 on the inner arc surface of the limiting semi-ring 606 fits with the outer ring surface in the middle of the test tube, the external motor 602 will stop moving. However, when the limiting semi-ring 606 moves, it will drive the L-shaped plate 608 fixed on its upper part to perform synchronous motion. Since the reciprocating spring 609 is fixed on the side wall of the inner cavity opened inside the transverse plate of the L-shaped plate 608, when the two sets of baffles 610 are against each other, they will move in the direction of the reciprocating spring 609. At this time, the reciprocating spring 609 is in a compressed state, and the two sets of baffles 610 will be against each other, and the bottom will fit with the top of the test tube. By fitting the bottom of the baffle 610 with the top of the test tube, the internal sample liquid of the test tube can be prevented from splashing when the test tube rotates, thereby avoiding damage to the experimental equipment caused by liquid splashing, such as corrosion, blockage, etc., thereby reducing the frequency of equipment maintenance and cleaning, thereby reducing the cost of microbial testing.

[0060] The above shows and describes the basic principles, main features and advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited to the above embodiments. The above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention. The scope of protection of the present invention is defined by the attached claims and their equivalents.

Claims

1. A multifunctional microorganism automatic testing device, comprising a liquid injection and dilution box (1), wherein an inner cavity is provided at the bottom of the liquid injection and dilution box (1), a shunt and drainage box (2) is fixedly connected to the side of the liquid injection and dilution box (1), an automatic testing box (3) is fixedly connected to the side of the shunt and drainage box (2), and a detection feedback box (4) is fixedly connected to the side of the automatic testing box (3); The injection and dilution box (1) is used to dilute the solution, the diversion and drainage box (2) distributes the solution in the test tube to different test tubes through the diversion mechanism, and discharges the excess liquid through the drainage mechanism, the automated inspection box (3) detects the solution after the diversion is completed, and the detection feedback box (4) performs real-time detection based on the data transmitted by the automated inspection box (3); A transmission dilution component (7) is arranged at the top of the inner cavity of the liquid injection and dilution box (1), and the transmission dilution component (7) includes an electric guide rail (701) fixedly connected to the top of the inner cavity of the liquid injection and dilution box (1), a sliding cylinder (702) is slidably connected inside the electric guide rail (701), and a pneumatic gripper (703) is fixedly connected to the output shaft of the sliding cylinder (702), a fixed plate (601) is fixedly connected to the bottom of the inner cavity of the liquid injection and dilution box (1), an electric telescopic rod (704) is fixedly connected to the top of the fixed plate (601), and a dilution head (705) is fixedly connected to the output end of the electric telescopic rod (704), characterized in that: A rotating defoaming mechanism (5) is provided in the inner cavity at the bottom of the liquid injection and dilution box (1); The rotating debubbling mechanism (5) comprises a forward and reverse motor (501) fixedly arranged at the bottom of the inner cavity of the liquid injection and dilution box (1); a rotating disc (502) is fixedly arranged on the output shaft of the forward and reverse motor (501); a plurality of groups of limiting plates (507) are slidably arranged on the upper part of the rotating disc (502); the test tube can be limited and rotated by the aggregation of the plurality of groups of limiting plates (507), so that bubbles in the solution can be removed, thereby improving the accuracy of the test result.

2. A multifunctional microbial automatic testing device according to claim 1, characterized in that: The upper part of the rotating disc (502) is fixedly connected with a connecting rod (503), one end of the connecting rod (503) is fixedly connected with a rotating base plate (504), a polygonal groove (505) is provided inside the rotating base plate (504), a displacement plate (506) is slidably connected inside the polygonal groove (505), the top of the displacement plate (506) is fixedly connected with a limiting plate (507), and the top of the limiting plate (507) is fixedly connected with a limiting column (508).

3. A multifunctional microbial automatic testing device according to claim 2, characterized in that: The rotation of the rotating base plate (504) drives the displacement plate (506), the limiting plate (507) and the limiting column (508) to move synchronously, and the movement of the rotating base plate (504) can provide power for subsequent equipment.

4. A multifunctional microbial automatic testing device according to claim 2, characterized in that: The outer ring surface of the rotating bottom plate (504) is sleeved with a rotating outer shell (509), the upper part of the rotating outer shell (509) is penetrated by a guide groove (510), the outer ring surface of the rotating outer shell (509) is provided with an arc-shaped opening (512), and the connecting rod (503) is slidably connected inside the arc-shaped opening (512).

5. A multifunctional microbial automatic testing device according to claim 4, characterized in that: The guide groove (510) can achieve the purpose of aggregation and diffusion through the limiting of the limiting column (508) when the limiting plate (507) moves, thereby limiting the outer annular surface of the test tube.

6. A multifunctional microbial automatic testing device according to claim 4, characterized in that: The outer ring surface of the rotating shell (509) is fixedly connected to a first oblique block (511), and the first oblique block (511) and the arc-shaped opening (512) are symmetrically distributed about the central axis of the rotating shell (509). The inner cavity side surface of the liquid injection and dilution box (1) is fixedly connected to a return spring (513), and one end of the return spring (513) is fixedly connected to a second oblique block (514).

7. A multifunctional microbial automatic testing device according to claim 6, characterized in that: The arrangement of the first oblique block (511) and the second oblique block (514) can prevent the rotating housing (509) from rotating before the limiting plate (507) is assembled, and the arrangement of the reset spring (513) will shrink when the side wall of the limiting plate (507) is in contact with the outer annular surface of the test tube, thereby driving the test tube to rotate synchronously.

8. The multifunctional microbial automatic testing device according to claim 1, characterized in that: A limiting shielding mechanism (6) for shielding is arranged at the bottom of the inner cavity of the injection and dilution box (1), and the limiting shielding mechanism (6) comprises an external motor (602), the external motor (602) is fixedly connected to the side wall of the fixed plate (601), the output shaft of the external motor (602) is fixedly connected to a rotating screw (603), the outer ring surface of the rotating screw (603) is threadedly connected to a reciprocating cylinder (604), the outer ring surface of the reciprocating cylinder (604) is provided with a limiting ring (605), the reciprocating cylinder (604) is slidably connected in the limiting ring (605), the rotating screw (603) passes through and is rotatably connected to the inside of the fixed plate (601), and one end of the limiting ring (605) is fixedly connected to the side of the fixed plate (601).

9. The multifunctional microbial automatic testing device according to claim 8, characterized in that: One end of the reciprocating cylinder (604) is fixedly connected to a limiting semi-ring (606), a groove is provided on the inner arc surface of the limiting semi-ring (606), a rotating column (607) is rotatably connected to the side wall of the groove on the inner arc surface of the limiting semi-ring (606), an L-shaped plate (608) is fixedly connected to the upper part of the limiting semi-ring (606), the L-shaped plate (608) is composed of a vertical plate and a horizontal plate, an inner cavity is provided inside the horizontal plate of the L-shaped plate (608), a reciprocating spring (609) is fixedly connected to the side wall of the inner cavity of the L-shaped plate (608), and one end of the reciprocating spring (609) is fixedly connected to a shielding plate (610).

10. The multifunctional microbial automatic testing device according to claim 9, characterized in that: The movement of the reciprocating cylinder (604) can limit the middle of the test tube through the limiting semi-ring (606) without hindering the operation of the transmission and dilution component (7). The rotating column (607) can provide support for the rotation of the test tube. The contraction of the shielding plate (610) can block the mouth of the test tube, thereby avoiding sample overflow, thereby reducing the cost of microbiological testing.

Images