Colorful bean puffball and application thereof in promoting growth and development of elm

Abstract

The invention discloses a Pisolithus tinctorius (Pisolithus tinctorius) and an application of the Pisolithus tinctorius in promoting growth and development of elm trees, and relates to the technical field of microorganisms. The coloured bean puffball is Pt and is preserved in the China General Microbiological Culture Collection Center on August 7, 2024, and the preservation number of the coloured bean puffball is CGMCC No. 41422. The method can generate obvious influence on elm growth and partial photosynthetic related parameters, effectively promotes growth and development of the host elm, provides a new choice for good growth of the elm, and is of great significance in wind prevention and sand fixation, soil quality improvement and ecological balance maintenance in current related areas.

Description

Technical Field

[0001] The invention relates to the technical field of microorganisms, and in particular to a colorful bean puffball (Pisolithus tinctorius) and application thereof in promoting the growth and development of elm. Background Art

[0002] Elm (Ulmuspumila L.) is a deciduous tree of the genus Ulmus in the family Ulmaceae. It is also known as domestic elm, white elm, and money elm. It is native to China, North Korea, Russia, Mongolia, and other places. It is widely distributed and is especially common in the provinces and regions of Northeast China, North China, Northwest China, and Southwest China. Elm has the characteristics of strong adaptability, excellent material, resistance to barrenness, drought, and salt and alkali. It is a good fast-growing broad-leaved material tree species, and grows well in deep, fertile, well-drained alluvial soil and the Loess Plateau. Therefore, it has also become an ideal protective afforestation tree species. In addition, elm is also one of the main tree species for afforestation in saline-alkali land. In afforestation in saline-alkali land, the role of elm is not only reflected in its adaptability to saline-alkali land, but also in its positive role in improving soil environment, preventing soil erosion, and increasing biodiversity.

[0003] Therefore, based on the characteristics of elm trees, how to promote their growth and development is of great significance to preventing wind and sand, improving soil quality and maintaining ecological balance in the current relevant areas.

[0004] Colored bean puffball is a medicinal fungus, which can be used for auxiliary treatment of traumatic bleeding, frostbite discharge, pus discharge and other diseases, and has high medicinal value. At present, there is no report that colored bean puffball can be used to promote the growth and development of elm. Summary of the invention

[0005] The invention discloses a colorful bean puffball, which can be used to promote the growth and development of elm trees and enable them to obtain better growth and development effects.

[0006] The technical solution adopted by the present invention is as follows:

[0007] One of the purposes of the present invention is to provide a colored bean puffball (Pisolithus tinctorius), wherein the colored bean puffball is Pt, which was deposited in the General Microbiological Center of China Culture Collection Administration on August 7, 2024, with a deposit number of CGMCC No.41422, and a deposit address of No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0008] Another object of the present invention is to provide the use of the colored bean puffball as a mycorrhizal fungus to promote the growth and development of elm.

[0009] The present invention has the following advantages and beneficial effects:

[0010] Experiments show that the colored bean puffball (preservation number: CGMCC No. 41422) provided by the present invention, as a mycorrhizal fungus, can significantly increase the plant height and ground diameter of elm through inoculation treatment, and also increase the Fo (initial fluorescence), chlorophyll a, chlorophyll b, total chlorophyll content, maximum net photosynthetic rate, transpiration rate and stomatal conductance of elm, and reduce the intercellular CO2 concentration. Therefore, the present invention can have a significant impact on the growth and photosynthesis-related parameters of elm, effectively promote the growth and development of the host elm, and provide a new choice for the good growth of elm, which is not only of great significance for windbreak and sand fixation, improving soil quality and maintaining ecological balance in the current relevant areas, but also because it is a root symbiotic fungus, it forms a mutually beneficial symbiosis with elm, thus retaining the original medicinal properties of the colored bean puffball, which can be said to kill two birds with one stone. BRIEF DESCRIPTION OF THE DRAWINGS

[0011] Figure 1 This is a schematic diagram of the effects of the colored bean puffball with the deposit number CGMCC No. 41422 in the embodiment of the present invention after inoculation and the blank control group on the ground diameter of elm seedlings.

[0012] Figure 2 This is a schematic diagram of the effects of the colored bean puffball with the deposit number CGMCC No. 41422 in the embodiment of the present invention on the plant height of elm seedlings after inoculation treatment and the blank control group.

[0013] Figure 3 This is a schematic diagram of the effects of the colored bean puffball with the deposit number CGMCC No. 41422 in the embodiment of the present invention after inoculation and the blank control group on the chlorophyll fluorescence parameters (Fo, Fm, Fv) of elm seedlings.

[0014] Figure 4 This is a schematic diagram of the effects of the colored bean puffball with the deposit number CGMCC No. 41422 in the embodiment of the present invention on the chlorophyll content of elm seedlings after inoculation treatment and the blank control group.

[0015] Figure 5 This is a schematic diagram of the effects of the colored bean puffball with the deposit number CGMCC No. 41422 in the embodiment of the present invention on the photosynthetic indexes of elm seedlings after inoculation treatment and the blank control group. DETAILED DESCRIPTION

[0016] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with various embodiments.

[0017] Example

[0018] This embodiment provides a colorful bean puffball, which is collected and separated from the root system of Pinus tabulaeformis, and its deposit number is CGMCC No.41422. It was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration on August 7, 2024, and its taxonomic name is Pisolithus tinctorius. The colorful bean puffball can be used to promote the growth and development of elm, and the specific process is as follows:

[0019] 1. Preparation of microbial agents

[0020] After activating the above-mentioned colorful bean puffball (hereinafter referred to as "preserved strain") with PDA culture medium, pick up a 6-mm-diameter cake from the edge of the vigorously growing colony and put it in 100 mL of liquid culture medium, and culture it at 25°C with constant temperature shaking for 21 days. Then, collect the mycelium, rinse it with sterile water, and crush it in a blender, and add sterile water to make a mycelium suspension.

[0021] 2. Cultivation of Elm Seeds Using Bacterial Agents

[0022] After germination, the disinfected elm seeds were placed in a sterilized planting medium, covered with 1 cm of soil and sprayed with sufficient distilled water. For potting, plastic pots (specifications: 20 cm × 18 cm) with water holes at the bottom were used, and 5 seedlings were planted in each pot. After culturing in a greenhouse (average temperature during the experiment was 26°C and average relative humidity was 40%) for 1 month, the above-mentioned bacterial agent was added to the root area of ​​the elm seedlings.

[0023] 3. Experimental comparison

[0024] According to the cultivation method described in the second point above, a blank control group was prepared. Unlike the microbial agent group, in the blank control group, after culturing for one month in a greenhouse (the average temperature during the experiment was 26°C and the average relative humidity was 40%), no microbial agent was added, but sterile water was used for spraying.

[0025] After the elm seedlings were cultivated for 6 months, the growth and physiological indicators of the elm seedlings in the inoculum group and the blank control group were measured, including plant height, ground diameter, chlorophyll fluorescence parameters, chlorophyll content and photosynthetic indicators.

[0026] in conclusion:

[0027] according to Figure 1 It can be seen that under the same culture time, the plant diameter at ground level of the fungus group (ECMF) was about 4.5 mm, while that of the blank control group (CK) was about 3.5 mm, with a difference of about 1 mm. This shows that after inoculation with the preserved fungus, the plant diameter at ground level of elm seedlings can be effectively promoted.

[0028] according to Figure 2It can be seen that under the same culture time, the plant height of the bacterial agent group (ECMF) was about 42.5 cm, while the plant height of the blank control group (CK) was about 32.5 cm, with a difference of about 10 cm. This shows that the plant height of the elm seedlings was greatly increased after inoculation with the preserved bacterial strains.

[0029] according to Figure 3 It can be seen that under the same culture time, the fungus group (ECMF) was higher than the blank control group (CK) in Fo (initial fluorescence), Fm (maximum fluorescence yield), and Fv (variable fluorescence). Among them, the increase in Fo value indicates that the ability of elm chlorophyll to absorb light energy was effectively improved after inoculation with the preserved bacteria; the Fv / Fm (PSII primary light energy conversion efficiency) of the fungus group (ECMF) was basically the same as that of the blank control group (CK), and both were within the normal range of 0.7-0.85, which shows that the inoculation with the preserved bacteria will not affect the health of the elm seedlings (the specific Fv / Fm value of the plant depends on the plant variety. The higher the Fv / Fm value, the lower the stress conditions and the better the health status; the lower the Fv / Fm value, the affected plant photosynthesis and the worse the health status under strong stress. The Fv / Fm value of normal plants is about 0.7-0.85).

[0030] according to Figure 4 It can be seen that under the same culture time, the chlorophyll a (unit: mg / g), chlorophyll b (unit: mg / g) and total chlorophyll content (unit: mg / g) of the bacterial agent group (ECMF) were higher than those of the blank control group (CK), which indicates that after inoculation with the preserved bacteria, the most important photosynthetic pigment (chlorophyll) index of elm seedlings was significantly improved.

[0031] according to Figure 5 It can be seen that under the same culture time, the maximum net photosynthetic rate (unit: μmol·m -2 ·s -1 ), stomatal conductance (unit: mmol·m -2 ·s -1 ), transpiration rate (unit: mmol·m -2 ·s -1 ) were higher than those in the blank control group (CK), while the intercellular CO2 concentration (unit: μmol·m -2 ·s -1 ) was lower than that of the blank control group (CK), which indicated that the photosynthetic indexes of elm seedlings were significantly improved after inoculation with the preserved strains.

[0032] The above results show that the colored bean puffball provided in this embodiment effectively promotes the growth of the host elm after inoculation; at the same time, since it is a root symbiotic fungus, the original medicinal properties of the colored bean puffball are retained.

[0033] The above-mentioned embodiments only express the specific implementation methods of the present application, and the descriptions thereof are relatively specific and detailed, but they cannot be understood as limiting the protection scope of the present application. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the technical solution concept of the present application, and these all belong to the protection scope of the present application.

Claims

1. A colored bean puffball (Pisolithus tinctorius), characterized in that: The colorful bean puffball is Pt, which was deposited in the General Microbiology Center of China Microorganism Culture Collection Administration on August 7, 2024, with the deposit number CGMCC No.41422, and the deposit address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

2. Application of the colored bean puffball described in claim 1 as mycorrhizal fungi to promote the growth and development of elm.

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