Sweet potato leaf whitening cream and preparation method thereof

Abstract

The invention provides sweet potato leaf whitening cream and a preparation method thereof. The sweet potato leaf whitening cream comprises traditional Chinese medicine whitening active ingredients, a humectant and water, the traditional Chinese medicine whitening active component is prepared from the following components in parts by mass: 5 to 15 parts of sweet potato leaves, 40 to 60 parts of radix angelicae, 40 to 60 parts of rhizoma bletillae, 10 to 20 parts of mirabilite, 20 to 40 parts of cortex moutan, 20 to 40 parts of flos carthami, 40 to 60 parts of spica prunellae, 10 to 30 parts of semen vaccariae, 20 to 40 parts of rhizoma atractylodis macrocephalae, 20 to 40 parts of poria cocos, 20 to 40 parts of rhizoma typhonii and 20 to 40 parts of pearl powder. The sweet potato leaves and the traditional Chinese medicinal materials are creatively combined to synergistically generate an excellent tyrosinase activity inhibiting effect, the prepared sweet potato leaf whitening cream has a good resisting effect on skin injury caused by ultraviolet rays, the problems of melanin pigmentation and the like caused by the ultraviolet rays and the like are effectively solved, the skin color is brightened, the darkness is improved, and the whitening effect is good. Meanwhile, the composition has the effects of relieving pachulosis and dryness of skin and the like, contributes to reducing ROS (reactive oxygen species) generation and is suitable for after-sun whitening and repairing of consumers.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemical products, and in particular to a sweet potato leaf whitening cream and a preparation method thereof. Background Art

[0002] Ultraviolet rays are an important factor that causes skin damage. Medium-wave ultraviolet rays (UVB) have high energy and can cause sunburn, dilation of capillaries, and erythema. Long-term exposure can cause proliferation of keratinocytes, leading to thickening, roughness, and wrinkles of the skin. Long-wave ultraviolet rays (UVA) have strong penetrating power and accelerate the formation of wrinkles and spots, leading to skin aging, and promote the formation of melanin in the skin, leading to skin pigmentation, inducing or aggravating various spots such as freckles and chloasma. Ultraviolet rays can induce skin cells to produce a large number of active oxygen free radicals, which further cause oxidative stress reactions and oxidative damage to important intracellular substances such as lipids, proteins, and DNA. If exposed to ultraviolet radiation for a long time, it will lead to a reduction in skin collagen fibers and the denaturation and deposition of elastin, causing the skin to lose elasticity, and problems such as wrinkles and sagging will occur, and even skin cancer may occur.

[0003] Sweet potato, also known as sweet potato, sweet potato, sweet potato, etc., is an annual plant of the Convolvulaceae family. my country is the country with the largest sweet potato planting area. As a by-product of sweet potato, sweet potato leaves have relatively small production benefits. According to statistics, only about 30% of sweet potato leaves are processed into food or feed, while in some areas they are discarded, causing problems such as resource waste and environmental pollution. Studies have shown that the polyphenol content and antioxidant activity of sweet potato leaves are higher than those of some common vegetables, and have certain utilization value. Summary of the invention

[0004] In view of this, the purpose of the present invention is to propose a sweet potato leaf whitening cream and a preparation method thereof. The present invention combines sweet potato leaves with Chinese medicinal materials such as Angelica dahurica, Bletilla striata, Sodium sulfate, Cortex Moutan, Carthamus tinctorius, Herba Prunellae, Vaccaria segetalis, Atractylodes macrocephala, Poria cocos and Radix Alba, and synergistically produces an excellent inhibitory effect on tyrosinase activity. The prepared sweet potato leaf whitening cream has a good antagonistic effect on skin damage caused by ultraviolet rays, thereby broadening the utilization methods of sweet potato leaves.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A sweet potato leaf whitening cream comprising a traditional Chinese medicine whitening active ingredient, a moisturizer and water;

[0007] The traditional Chinese medicine whitening active ingredients, measured by weight, include: 5-15 parts of sweet potato leaves, 40-60 parts of angelica dahurica, 40-60 parts of bletilla striata, 10-20 parts of mirabilite, 20-40 parts of moutan bark, 20-40 parts of safflower, 40-60 parts of selfheal, 10-30 parts of fenugreek, 20-40 parts of atractylodes macrocephala, 20-40 parts of poria, 20-40 parts of white aconite and 20-40 parts of pearl powder.

[0008] Preferably, based on weight, the ingredients include: 8-12 parts of sweet potato leaves, 45-55 parts of Angelica dahurica, 45-55 parts of Bletilla striata, 10-20 parts of Sodium Sulfate, 25-35 parts of Moutan Bark, 25-35 parts of Carthamus tinctorius, 45-55 parts of Prunella Vulgaris, 15-25 parts of Vaccaria segetalis, 25-35 parts of Atractylodes macrocephala, 25-35 parts of Poria cocos, 25-35 parts of Radix Rubrae Chinensis and 25-35 parts of pearl powder.

[0009] More preferably, in parts by mass, it includes: 8-12 parts of sweet potato leaves, 48-52 parts of Angelica dahurica, 48-52 parts of Bletilla striata, 13-17 parts of Sodium Sulfate, 28-32 parts of Moutan Bark, 28-32 parts of Carthamus tinctorius, 48-52 parts of Prunella Vulgaris, 18-22 parts of Vaccaria segetalis, 28-32 parts of Atractylodes macrocephala, 28-32 parts of Poria cocos, 28-32 parts of Radix Rubrae Chinensis and 28-32 parts of pearl powder.

[0010] Sweet potato leaves, Ipomoea batatas (L.) Lam., are perennial herbaceous creeping plants of the genus Morning Glory in the Convolvulaceae family. Sweet potato leaves are mild in nature, sweet in taste, non-toxic, and have the effects of tonifying deficiency and replenishing qi, strengthening the spleen and kidneys, benefiting the lungs and promoting fluid production, tonifying the kidneys and improving eyesight, anti-cancer, beautifying, and delaying aging.

[0011] Angelica dahurica is the dried root of Angelica dahurica (Fisch.ex Hoffm.) Benth.etHook.f. or Angelica dahurica (Fisch.ex Hoffm.) Benth.etHook.f.var.formosana (Boiss.) Shan et Yuan, a plant of the Umbelliferae family. It contains chemical components such as isoimperatorin, imperatorin, bergapten, phellopterin, and oxypeucedanin. It is pungent and warm in nature. Pharmacological studies have shown that it has anti-inflammatory, antipyretic, analgesic, and antibacterial effects.

[0012] Bletilla striata, also known as Baiji, is the dried tuber of Bletilla striata (Thunb.) Reichb.f. of the orchid family. The tuber contains mucilage and starch, and can be used as a paste or medicine. It is bitter, sweet, astringent, and slightly cold in nature. It has the effects of astringing and stopping bleeding, reducing swelling and promoting tissue regeneration.

[0013] Mirabilite is a crystal made by processing and refining the sulfate mineral mirabilite. It is salty, bitter and cold in nature. Its functions include purging heat and relieving constipation, moistening dryness and softening hard masses, clearing away heat and reducing swelling. It is used for constipation due to excess heat, dry stool, abdominal pain due to stagnation, and swelling and pain due to intestinal carbuncle; it is also used for external treatment of breast carbuncle and swelling and pain due to hemorrhoids.

[0014] Peony bark, also known as moutan bark, powdered moutan bark, wood peony, strip moutan bark, Luoyang flower, is the dried root bark of Paeonia suffruticosa Andr., a plant of the Ranunculaceae family. The root contains peony phenol, peony glycoside, peony glycoside, and peony glycoside. It also contains 0.15-0.4% volatile oil and phytosterols. It is bitter, pungent, and slightly cold in nature. Functions and indications: clearing heat and cooling blood, promoting blood circulation and removing blood stasis. It is used for fever and toxicity, vomiting blood and epistaxis, night heat and morning coolness, no sweat and bone steaming, amenorrhea and dysmenorrhea, carbuncle, sore, and pain from falls.

[0015] Safflower is the dried flower of Carthamus tinctorius L., a plant of the Asteraceae family. Safflower contains safflower yellow pigment and safflower glycosides. Safflower glycosides are hydrolyzed with hydrochloric acid to obtain glucose and safflower pigment. It also contains 15α, 20β-dihydroxy-Δ4-pregnen-3-one. It also contains fatty oil called safflower oil, which is a glyceride of palmitic acid, stearic acid, arachidic acid, oleic acid, linoleic acid, linolenic acid, etc. It is pungent in nature and warm in flavor. Functions and indications: promoting blood circulation and dredges the menstruation, dispersing blood stasis and relieving pain. It is used for amenorrhea, dysmenorrhea, lochia retention, masses and lumps, injuries from falls, and swelling and pain in sores.

[0016] Prunella vulgaris is the dried fruit spike of Prunella vulgaris L., a plant of the Labiatae family. It is pungent, bitter and cold in nature. The whole plant contains triterpenoid saponins, the aglycone of which is oleanolic acid, as well as free oleanolic acid, ursolic acid, rutin, hyperoside, cis-caffeic acid, trans-caffeic acid, vitamin B1, vitamin C, vitamin K, carotene, resin, bitter substances, tannins, volatile oils, alkaloids, water-soluble salts (about 3.5%, of which about 63% is potassium chloride), etc. Functions and indications: clearing heat, improving eyesight, dispersing nodules, and reducing swelling. It is used for red and swollen eyes, night pain in the eyeballs, headache and dizziness, scrofula, goiter, breast abscess and swelling; goiter, lymph node tuberculosis, breast hyperplasia, and hypertension.

[0017] Vaccaria segetalis (Neck.) Garcke is the dried mature seeds of Vaccaria segetalis (Neck.) Garcke, a plant of the Caryophyllaceae family. Its chemical components include vacsegoside and vaccarin, etc. It is bitter in nature and flavor, flat in nature. Its functions and indications are: promoting blood circulation and relieving menstruation, promoting lactation and reducing swelling. It is used for lactation retention, amenorrhea, dysmenorrhea, and mastitis and swelling.

[0018] Poria cocos is the dried sclerotium of the Polyporaceae fungus Poria cocos (Schw.) Wolf. The sclerotium contains about 93% of β-poria polysaccharide by dry weight and triterpenoid compounds such as acetyl-poria cocos acid, pachymic acid, and 3β-hydroxylanostearic acid. In addition, it also contains gum, chitin, protein, fat, sterol, lecithin, glucose, adenine, histidine, choline, β-poria polysaccharide degrading enzyme, lipase, protease, etc. It is sweet, light, and flat in nature. Functions and indications: diuresis and dampness elimination, spleen-strengthening and heart-calming. It is used for edema, oliguria, phlegm and fluid retention, dizziness and palpitations, spleen deficiency and poor appetite, loose stools and diarrhea, restlessness, palpitations and insomnia.

[0019] Atractylodes macrocephala is the dried rhizome of Atractylodes macrocephala Koidz. of the Asteraceae family. It is bitter, sweet and warm in nature. Its chemical composition contains 1.4% volatile oil, the main components of which are atractylol and atractylone, and contains vitamin A. Functions and indications: invigorating the spleen and replenishing qi, drying dampness and promoting diuresis, stopping sweating, and calming the fetus. It is used for spleen deficiency, poor appetite, abdominal distension and diarrhea, phlegm and fluid, dizziness and palpitations, edema, spontaneous sweating, and restless fetus. Atractylodes macrocephala strengthens the spleen, harmonizes the stomach, and calms the fetus. It is used for spleen deficiency, poor appetite, diarrhea, loose stools, and restless fetus.

[0020] White aconite root is the dried tuber of Typhonium giganteum Engl., a plant of the Araceae family. The tuber contains β-sitosterol, β-sitosterol-D-glucoside, meso-inositol, choline, uracil, succinic acid, tyrosine, valine, palmitic acid, linoleic acid, oleic acid, linolein, and dipalmitin. It also contains typhonium giganteum lectin. It is pungent and warm in nature and is toxic. Functions and indications: dispelling wind and phlegm, calming convulsions, detoxifying, dispersing knots, and relieving pain. It is used for stroke with phlegm congestion, facial paralysis, slurred speech, phlegm-induced headache, hemicrania, laryngeal congestion and sore throat, tetanus; external treatment for scrofula, phlegm nodules, and poisonous snake bites.

[0021] Preferably, it also includes 8 to 12 parts of mint and 0.5 to 2 parts of borneol.

[0022] Preferably, the sweet potato leaves are freeze-dried sweet potato leaves powder, and the preparation method of the freeze-dried sweet potato leaves powder comprises:

[0023] The sweet potato leaves are crushed, added with water, mixed, refluxed for extraction, filtered, the filtrate is collected, concentrated, and then freeze-dried to obtain the sweet potato leaf freeze-dried powder;

[0024] The solid-liquid ratio of the sweet potato leaves to water is 1:6-15 g / mL; the temperature of the reflux extraction is 70-75° C., the extraction time is 1-2 hours, and the reflux extraction is repeated 2-3 times.

[0025] In one embodiment of the present invention, the present invention provides a preferred method for preparing freeze-dried sweet potato leaf powder, as follows:

[0026] Take 100 g of sweet potato leaves, crush them, add 800 mL of deionized water, perform reflux extraction at 80° C. for 1 h, repeat the reflux extraction twice, filter, take the filtrate, perform rotary concentration at 70° C., and then freeze-dry to obtain sweet potato leaf freeze-dried powder.

[0027] Preferably, the preparation method of the whitening active ingredient of traditional Chinese medicine comprises:

[0028] (1) crushing sweet potato leaves, adding water to mix, refluxing extraction, filtering, collecting the filtrate, concentrating, and then freeze-drying to obtain sweet potato leaf freeze-dried powder;

[0029] The mass volume ratio of the sweet potato leaves to water is 1:6-15 g / mL; the temperature of the reflux extraction is 70-75° C., the extraction time is 1-2 hours, and the reflux extraction is repeated 2-3 times;

[0030] (2) taking angelica dahurica, bletilla striata, mirabilite, moutan bark, safflower, selfheal, fenugreek, atractylodes macrocephala, poria and white aconite, obtaining the medicinal materials, crushing them, adding water for soaking and extraction, then decocting, filtering, taking the filtrate, and concentrating it to 4% to 6% of the total volume of the original filtrate to obtain the extracted Chinese medicinal liquid;

[0031] The weight ratio of the medicinal material to water is 1:10-15; the soaking extraction time is 2-3 hours;

[0032] (3) Adding the freeze-dried powder of sweet potato leaves and pearl powder to the extracted Chinese medicine solution and stirring, thereby obtaining the whitening active ingredient of Chinese medicine.

[0033] In one embodiment of the present invention, the present invention further selects mint and borneol as new components of traditional Chinese medicine whitening active ingredients for soothing effect. The present invention provides a preferred method for preparing traditional Chinese medicine whitening active ingredients, as follows:

[0034] (1) 100 g of sweet potato leaves were taken, crushed, added with 800 mL of deionized water, refluxed at 80° C. for 1 h, repeated refluxed twice, filtered, and the filtrate was taken, rotary concentrated at 70° C., and then freeze-dried to obtain freeze-dried sweet potato leaf powder;

[0035] (2) Take Angelica dahurica, Bletilla striata, Sodium sulfate, Cortex Moutan, Carthamus tinctorius, Herba Prunellae, Vaccaria segetalis, Atractylodes macrocephala, Poria cocos and Rhizoma Cynoglossi, grind them, add 10 times the weight of water to soak and extract for 2 hours, then decoct, filter, take the filtrate, and concentrate it by rotary concentrating at 70° C. to 4% to 6% of the original volume to obtain an extracted Chinese medicine solution;

[0036] (3) crushing mint and borneol, adding 3 times the weight of anhydrous ethanol and soaking for 3 hours to obtain an soaking extract;

[0037] (4) Adding the freeze-dried powder of sweet potato leaves and pearl powder to the extracted Chinese medicine solution, stirring, and adding the soaking extract solution to obtain the whitening active ingredient of Chinese medicine.

[0038] In one aspect, the present invention provides a method for preparing a sweet potato leaf whitening cream, comprising the following steps:

[0039] Formula S1: The amount of the whitening active ingredient of traditional Chinese medicine added is 3-8% by mass percentage;

[0040] S2 mixing: mix the moisturizer and water, stir, add the whitening active ingredient of traditional Chinese medicine, continue stirring, and obtain the sweet potato leaf whitening cream.

[0041] In one embodiment of the present invention, the present invention further selects emollients [ORYZA SATIVA (RICE) BRAN OIL], moisturizers (GLYCERIN, SODIUM HYALURONATE), emulsifiers (TRIETHANOLAMINE, GLYCERYL STEARATE) and solvents (deionized water) as the formula of the sweet potato leaf whitening cream. The present invention provides a preferred method for preparing the sweet potato leaf whitening cream, as follows:

[0042] (1) Formula: 5% of traditional Chinese medicine whitening active ingredient, 2% of rice (ORYZA SATIVA) bran oil, 3% of glycerin, 2% of sodium hyaluronate, 2% of triethanolamine, 2% of glyceryl stearate and 84% of deionized water;

[0043] (2) According to the formula, rice bran oil and deionized water are mixed, heated to 70-75° C., and stirred to obtain an emulsion;

[0044] (3) Add the active whitening ingredients of traditional Chinese medicine to the emulsion, stir to 30-35° C., add glycerin, sodium hyaluronate, triethanolamine and glyceryl stearate, continue stirring to obtain the sweet potato leaf whitening cream.

[0045] On the other hand, the present invention also provides the use of the above-mentioned sweet potato leaf whitening cream in inhibiting tyrosinase activity.

[0046] The present invention also provides application of the sweet potato leaf whitening cream in whitening and repairing after sun exposure.

[0047] Preferably, the sweet potato leaf whitening cream is used to inhibit melanin deposition and / or increase the SOD enzyme content in skin tissue after sun exposure.

[0048] Compared with the prior art, the present invention has the following beneficial effects:

[0049] The prescription is based on harmonizing qi and blood, removing blood stasis and turbidity, strengthening the spleen and removing dampness. It combines the methods of dispelling wind and dispersing knots, clearing heat and cooling blood to comprehensively improve the skin's metabolic function and achieve whitening and anti-aging effects. The medicinal properties are both cold and warm, and both tonifying and purging are used, reflecting the idea of ​​yin and yang balance in traditional Chinese medicine.

[0050] The present invention creatively combines sweet potato leaves with Chinese medicinal materials such as angelica dahurica, bletilla striata, mirabilite, moutan bark, safflower, selfheal, fenugreek, atractylodes macrocephala, tuckahoe and white aconite, and synergistically produces an excellent inhibitory effect on tyrosinase activity. The prepared sweet potato leaf whitening cream has a good antagonistic effect on skin damage caused by ultraviolet rays, effectively solves problems such as melanin deposition caused by ultraviolet rays, brightens skin tone, improves dullness, and has the effects of relieving rough and dry skin, etc., helps to reduce ROS generation, and is suitable for consumers to whiten and repair their skin after sun exposure.

[0051] In combination with the test examples of the present invention, the present invention combines the freeze-dried sweet potato leaf powder with Chinese medicinal materials such as Angelica dahurica, Bletilla striata, Sodium sulfate, Cortex Moutan, Carthami, Prunella Vulgaris, Vaccaria segetalis, Atractylodes macrocephala, Poria cocos and Rhizoma Alba. In a mouse melanoma B16 cell test, the tyrosinase activity is only 21.7%, and the inhibition rate reaches 78.3%, which effectively improves the whitening effect; in a guinea pig skin pigmentation model test, the skin L value is improved well, from 39.82 after modeling to 47.06, and the skin roughness is alleviated. Compared with the model group, the SOD enzyme content in the skin tissue is increased by 45.74%, which can resist the damage of ultraviolet rays to the skin. When the sweet potato leaves are replaced or lacking, the effect is greatly reduced. It can be seen that the sweet potato leaves have a certain synergistic effect on improving the whitening and anti-aging effects of Chinese medicinal materials. DETAILED DESCRIPTION

[0052] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0053] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0054] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0055] Mouse melanoma B16 cells were purchased from Beijing Beina Chuanglian Biotechnology Research Institute.

[0056] In the cytological experiment, different concentrations of the drug solution (5-150 mg / mL) did not change the morphology of B16 cells. Cell viability assays showed that this concentration range had no toxic effect on B16 cells and that B16 cells grew well.

[0057] Example 1

[0058] 10 parts of sweet potato leaves, 50 parts of Angelica dahurica, 50 parts of Bletilla striata, 15 parts of Sodium Sulfate, 30 parts of Cortex Moutan, 30 parts of Carthamus tinctorius, 50 parts of Herba Prunellae, 10 parts of Menthus, 1 part of Borneolum Syntheticum, 20 parts of Vaccaria segetalis, 30 parts of Atractylodes macrocephala, 30 parts of Poria, 30 parts of Radix White Rhizoma and 30 parts of Pearl Powder.

[0059] Example 2

[0060] 8 parts of sweet potato leaves, 55 parts of angelica dahurica, 55 parts of bletilla striata, 20 parts of sodium sulfate, 35 parts of moutan bark, 35 parts of safflower, 55 parts of selfheal, 10 parts of mint, 1 part of borneol, 25 parts of fenugreek, 35 parts of atractylodes macrocephala, 35 parts of tuckahoe, 35 parts of white aconite and 35 parts of pearl powder.

[0061] Example 3

[0062] 12 parts of sweet potato leaves, 45 parts of Angelica dahurica, 45 parts of Bletilla striata, 10 parts of Sodium Sulfate, 25 parts of Cortex Moutan, 25 parts of Carthamus tinctorius, 45 parts of Herba Prunellae, 10 parts of Menthus alba, 1 part of Borneolum Syntheticum, 15 parts of Vaccaria segetalis, 25 parts of Atractylodes macrocephala, 25 parts of Poria, 25 parts of Radix Rubrae and 25 parts of Pearl Powder.

[0063] Example 4

[0064] 8 parts of sweet potato leaves, 55 parts of Angelica dahurica, 55 parts of Bletilla striata, 15 parts of Sodium Sulfate, 40 parts of Cortex Moutan, 30 parts of Carthamus tinctorius, 50 parts of Herba Prunellae, 10 parts of Menthus alba, 1 part of Borneolum Syntheticum, 20 parts of Vaccaria segetalis, 55 parts of Atractylodes macrocephala, 40 parts of Poria, 40 parts of Radix Rubrae and 30 parts of Pearl Powder.

[0065] The preparation methods of the traditional Chinese medicine whitening active ingredients of Examples 1 to 4 are as follows:

[0066] (1) 100 g of sweet potato leaves were taken, crushed, added with 800 mL of deionized water, refluxed at 80° C. for 1 h, repeated refluxed twice, filtered, and the filtrate was taken, rotary concentrated at 70° C., and then freeze-dried to obtain freeze-dried sweet potato leaf powder;

[0067] (2) Take Angelica dahurica, Bletilla striata, Sodium sulfate, Cortex Moutan, Carthamus tinctorius, Herba Prunellae, Vaccaria segetalis, Atractylodes macrocephala, Poria cocos and Rhizoma Cynoglossi, grind them, add 10 times the weight of water to soak and extract for 2 hours, then decoct, filter, take the filtrate, and concentrate it by rotary concentrating at 70° C. to 4% to 6% of the original volume to obtain an extracted Chinese medicine solution;

[0068] (3) crushing mint and borneol, adding 3 times the weight of anhydrous ethanol and soaking for 3 hours to obtain an soaking extract;

[0069] (4) Adding the freeze-dried powder of sweet potato leaves and pearl powder to the extracted Chinese medicine solution, stirring, and adding the soaking extract solution to obtain the whitening active ingredient of Chinese medicine.

[0070] Comparative Example 1

[0071] The difference between this comparative example and Example 1 is that the dosage of each component is adjusted, and the specific formula is as follows: 4 parts of sweet potato leaves, 30 parts of Angelica dahurica, 30 parts of Bletilla striata, 15 parts of Sodium Sulfate, 50 parts of Moutan Bark, 30 parts of Carthamus tinctorius, 50 parts of Herba Prunellae, 10 parts of Menthus alba, 1 part of Borneolum Syntheticum, 20 parts of Vaccaria segetalis, 50 parts of Atractylodes macrocephala, 30 parts of Poria, 30 parts of Radix Rubrae and 30 parts of Pearl Powder.

[0072] Comparative Example 2

[0073] The difference between this comparative example and Example 1 is that the sweet potato leaves are missing, and the specific formula is as follows: 50 parts of Angelica dahurica, 50 parts of Bletilla striata, 15 parts of Sodium Sulfate, 30 parts of Cortex Moutan, 30 parts of Carthamus tinctorius, 50 parts of Herba Prunellae, 10 parts of Menthus alba, 1 part of Borneolum Syntheticum, 30 parts of Vaccaria segetalis, 30 parts of Atractylodes macrocephala, 30 parts of Poria cocos, 30 parts of Radix Rubrae and 30 parts of Pearl Powder.

[0074] Comparative Example 3

[0075] The difference between this comparative example and Example 1 is that the sweet potato leaves are replaced by marigolds, and the specific formula is as follows: 10 parts of marigolds, 50 parts of angelica dahurica, 50 parts of bletilla striata, 15 parts of sodium sulfate, 30 parts of moutan bark, 30 parts of safflower, 50 parts of selfheal, 10 parts of mint, 1 part of borneol, 20 parts of fenugreek, 30 parts of atractylodes macrocephala, 30 parts of poria, 30 parts of white aconite and 30 parts of pearl powder.

[0076] The preparation methods of the traditional Chinese medicine whitening active ingredients in Comparative Examples 1 to 3 are the same as those in Examples 1 to 4, wherein the marigold in Comparative Example 3 is freeze-dried powder, and the preparation method is the same as the preparation method of the freeze-dried sweet potato leaf powder in Examples 1 to 4.

[0077] Test Example 1

[0078] Mouse melanoma B16 cells were used as the research object. The B16 cell culture cells were restored to the state of melanin synthesis. The active ingredients of traditional Chinese medicine whitening were dissolved in PBS solution to obtain a drug solution (80 mg / mL), which was added to the B16 cell culture medium and incubated for 24 hours. An equal amount of PBS solution was replaced as the control group. After incubation, the supernatant was added to a 96-well plate, and 5 mM L-Dopa solution was added. After mixing, the plate was placed in a constant temperature water bath at 37°C for 10 minutes. The absorbance of each well was measured at a wavelength of 475 nm. The intracellular tyrosinase activity (%) = A0 / A 1 × 100%, where A 0 is the average absorbance value of the drug solution group, A 1 is the average absorbance value of the control group, and phenylethyl resorcinol (377) is used as the positive control.

[0079] Table 1 Results of cell tyrosinase activity

[0080] project 377 Example 1 Example 4 Comparative Example 1 Comparative Example 2 Comparative Example 3 Tyrosinase activity 55.2％ 21.7％ 29.0％ 36.0％ 42.6％ 38.5％

[0081] As can be seen from Table 1, the traditional Chinese medicine whitening active ingredients prepared in the examples and comparative examples have different degrees of inhibitory effects on the tyrosinase activity of B16 cells. Example 1 has the best inhibitory effect, with a tyrosinase activity of only 21.7%, followed by Example 4 with an activity of 29.0%. Compared with the comparative example, the traditional Chinese medicine whitening active ingredients provided in the examples of the present invention have a higher inhibitory effect on the cell tyrosinase activity.

[0082] Example 5

[0083] The preparation method of the sweet potato leaf whitening cream of Examples 1 to 4 is as follows:

[0084] (1) Formula: 5% of traditional Chinese medicine whitening active ingredient, 2% of rice (ORYZA SATIVA) bran oil, 3% of glycerin, 2% of sodium hyaluronate, 2% of triethanolamine, 2% of glyceryl stearate and 84% of deionized water;

[0085] (2) According to the formula, rice bran oil and deionized water are mixed, heated to 70-75° C., and stirred to obtain an emulsion;

[0086] (3) Add the active whitening ingredients of traditional Chinese medicine to the emulsion, stir to 30-35° C., add glycerin, sodium hyaluronate, triethanolamine and glyceryl stearate, continue stirring to obtain the sweet potato leaf whitening cream.

[0087] Comparative Example 6

[0088] The preparation methods of the whitening creams of Comparative Examples 1 to 3 are the same as those of Example 5.

[0089] Comparative Example 1

[0090] The preparation method of this reference example whitening cream is as follows:

[0091] (1) Formulation: 2% rice bran oil, 3% glycerin, 2% sodium hyaluronate, 2% triethanolamine, 2% glyceryl stearate and 89% deionized water;

[0092] (2) According to the formula, rice bran oil and deionized water are mixed, heated to 70-75° C., and stirred to obtain an emulsion;

[0093] (3) When the temperature of the emulsion drops to 30-35°C, add glycerin, sodium hyaluronate, triethanolamine and glyceryl stearate into the emulsion and continue stirring to obtain a whitening cream.

[0094] Test Example 2

[0095] 1. Guinea pig skin coloration model

[0096] Male guinea pigs were used as the research subjects, and the eighth edition of the Guide for the Care and Use of Laboratory Animals was followed. Eighteen healthy male guinea pigs weighing 250 to 300 g were randomly divided into six groups, with three in each group. After adaptive feeding, the backs of three guinea pigs were depilated, three were left untreated, and 15 were exposed to 400 mJ / cm 2 The irradiation energy was 308nm ultraviolet light, which was used to irradiate the hair removal area on the back once a day for 1 week to establish a skin pigmentation model.

[0097] Guinea pigs with uniform pigmentation on the back were selected, among which Group 1 was not treated in any way, Group 2 was smeared with the whitening cream of Example 1, Group 3 was smeared with the whitening cream of Example 4, Group 4 was smeared with the whitening cream of Comparative Example 2, Group 5 was smeared with the whitening cream of Comparative Example 3, and the 3 guinea pigs without modeling were smeared with the whitening cream of Comparative Example 1. The whitening cream was applied once a day, and the hair was shaved every 3 days before applying the medicine. The skin L value was detected by a colorimeter 3 weeks after application. The larger the L value, the more white the skin color is, and the smaller the L value, the more black the skin color is, that is, dark.

[0098] Table 2 Results of skin L value after modeling

[0099] project Blank group Model Group No. 2 No.3 No. 4 No. 5 L value 60.27 36.16 39.82 38.02 38.62 37.12

[0100] Table 3 Results of skin L value after 3 weeks of application

[0101] project Blank group Model Group No. 2 No.3 No. 4 No. 5 L value 61.46 30.51 47.06 42.37 34.72 36.87

[0102] As shown in Table 2, the L value of the guinea pig skin in the blank group was 60.27. After modeling, the skin of each group showed obvious pigmentation, dark skin, and rough skin.

[0103] As shown in Table 3, the skin L value of the model group was 28.51, indicating that pigmentation still existed in the skin without treatment at 3 weeks. The skin L values ​​of the applied drugs were improved to varying degrees. Compared with the drug solutions of No. 2 377 and No. 4-6 of Example 1, No. 5 had a better effect on improving the skin L value, had the best whitening activity, and alleviated the skin roughness.

[0104] At the end of the experiment, each group of guinea pigs was killed, and skin tissue of about 1 cm × 1 cm area was cut, excess fat was removed, and physiological saline was added for homogenization on ice. After low-temperature centrifugation, the supernatant was taken to obtain the test solution, and the superoxide dismutase (SOD) enzyme content in the skin tissue was determined using a superoxide dismutase (SOD) detection kit.

[0105] Table 4 Results of SOD content in skin tissue

[0106] project Blank group Model Group No. 2 No.3 No. 4 No. 5 <![CDATA[SOD(U·mgprot -1 )]]> 3.87 1.88 2.74 2.60 2.36 2.41

[0107] As shown in Table 4, compared with the blank group, after a certain period of ultraviolet irradiation of the guinea pig skin, the SOD enzyme content of the model group decreased significantly. After the application of different whitening creams, the SOD enzyme content changed to varying degrees. Among them, the SOD enzyme content of Group 2 increased the most, indicating that the whitening cream in Group 2 has a better effect on the skin damage caused by ultraviolet rays, increasing the SOD enzyme in the skin tissue, helping to reduce the production of ROS, and playing a whitening role in inhibiting melanin deposition. It is also suitable for whitening and repair after sun exposure.

[0108] 2. Combination ideas and overall effects

[0109] This combination is centered on harmonizing qi and blood, removing blood stasis and turbidity, strengthening the spleen and removing dampness. It combines the methods of dispelling wind and dispersing knots, clearing heat and cooling blood to comprehensively improve the skin's metabolic function and achieve whitening and anti-aging effects. The medicinal properties are both cold and warm, and both tonifying and purging are used, reflecting the idea of ​​yin and yang balance in traditional Chinese medicine.

[0110] (1) Drug classification and synergistic mechanism

[0111] 1. Wind-clearing and collateral-dredging group (improving the circulation of qi and blood)

[0112] Angelica dahurica: pungent and warm, it opens the orifices, dispels wind and dampness, disperses turbid evil from the head and face, and promotes skin microcirculation.

[0113] White Aconite: It is pungent and warm in nature, and can dispel wind and phlegm, resolve stagnation, and lead drugs to the surface. It is especially good at eliminating facial edema and pigmentation.

[0114] Fenugreek: bitter, mild and laxative, it can promote blood circulation and menstruation, unclog pores and promote the excretion of metabolic waste.

[0115] 2. Cooling blood and removing blood stasis group (clearing pathological products)

[0116] Cortex Moutan: slightly cold and enters the blood, clears blood heat, resolves blood stasis, and inhibits excessive deposition of melanin.

[0117] Safflower: pungent and warm in nature, it invigorates blood circulation, and complements the cold and warm properties of Cortex Moutan, improving dull skin due to blood stasis.

[0118] Prunella Vulgaris: Bitter and cold, it clears liver fire, relieves depression, and is effective for acne and pigmentation caused by liver depression turning into fire.

[0119] 3. Strengthening the spleen and eliminating dampness group (strengthening the foundation and nourishing the essence)

[0120] Atractylodes macrocephala: sweet and warm, it strengthens the spleen, transports and transforms water and dampness, and improves sallow and loose skin caused by spleen deficiency and dampness.

[0121] Poria: It has the effect of diuresis and dampness-eliminating, soothing the mind, regulating water metabolism, and reducing puffiness and oiliness of the skin.

[0122] 4. Muscle-reinforcing and regenerative group (barrier repair)

[0123] Bletilla striata: bitter, sweet, astringent and cold, astringent and hemostatic, promotes epidermal cell regeneration, and repairs photoaging damage.

[0124] Sodium Sulfate: It is salty, cold and can soften hard masses. It can clear away heat and reduce swelling when applied externally, and can be taken internally (with caution) to clear the bowels, detoxify and reduce toxin accumulation.

[0125] 5. Antioxidant group (anti-aging basis)

[0126] Sweet potato leaves: sweet and mild, rich in vitamins and polyphenols, can neutralize free radicals and delay cell aging.

[0127] (2) Theoretical basis of traditional Chinese medicine for whitening and anti-aging

[0128] 1. Whitening mechanism

[0129] Dispel wind and expel evil: Angelica dahurica and Radix Aconiti Lateralis Preparata can dispel wind evil on the face and reduce pigmentation caused by the invasion of external evil (ultraviolet rays, wind and dryness).

[0130] Cooling blood and removing blood stasis: Cortex Moutan and Carthamus tinctorius regulate blood and improve chloasma and dullness caused by microcirculation disorders.

[0131] Strengthen the spleen and remove dampness: Atractylodes macrocephala and Poria cocos improve the dull complexion caused by spleen deficiency and dampness, and enhance the source of Qi and blood metabolism.

[0132] 2. Anti-aging mechanism

[0133] Promoting blood circulation and unblocking meridians: Safflower and Vaccaria segetalis promote the circulation of Qi and blood, and delay the loss of skin collagen.

[0134] Healing sores and promoting skin regeneration: Bletilla striata and sweet potato leaves repair the epidermal barrier and reduce the formation of wrinkles.

[0135] Cleansing the liver and detoxifying: Prunella vulgaris and Glauber's salt remove internal heat toxins and delay oxidative stress damage to the skin.

[0136] (3) Modern pharmacological evidence

[0137] Inhibit tyrosinase: The active ingredients in Angelica dahurica and Bletilla striata can reduce melanin synthesis.

[0138] Antioxidant: The polyphenols and paeonol in sweet potato leaves have the effect of scavenging free radicals.

[0139] Anti-inflammatory regulation: Ursolic acid and safflower yellow pigment in Prunella vulgaris can inhibit skin inflammatory response.

[0140] Promote collagen synthesis: Poria polysaccharide and Atractylodes lactone can enhance the activity of skin elastic fibers.

[0141] These medicines work together to regulate blood, remove blood stasis, strengthen the spleen and eliminate dampness.

[0142] The above is a preferred embodiment of the present invention. It should be pointed out that a person skilled in the art can make several improvements and modifications without departing from the principle of the present invention. These improvements and modifications are also considered to be within the scope of protection of the present invention.

Claims

1. A sweet potato leaf whitening cream, characterized in that: Includes Chinese herbal whitening active ingredients, moisturizers and water; The traditional Chinese medicine whitening active ingredients, measured by weight, include: 5-15 parts of sweet potato leaves, 40-60 parts of angelica dahurica, 40-60 parts of bletilla striata, 10-20 parts of mirabilite, 20-40 parts of moutan bark, 20-40 parts of safflower, 40-60 parts of selfheal, 10-30 parts of fenugreek, 20-40 parts of atractylodes macrocephala, 20-40 parts of poria, 20-40 parts of white aconite and 20-40 parts of pearl powder.

2. A sweet potato leaf whitening cream according to claim 1, characterized in that: The invention comprises, by weight, 8-12 parts of sweet potato leaves, 45-55 parts of angelica dahurica, 45-55 parts of bletilla striata, 10-20 parts of sodium sulfate, 25-35 parts of moutan bark, 25-35 parts of safflower, 45-55 parts of selfheal, 15-25 parts of fenugreek, 25-35 parts of atractylodes macrocephala, 25-35 parts of poria, 25-35 parts of white aconite and 25-35 parts of pearl powder.

3. A sweet potato leaf whitening cream according to claim 2, characterized in that: Calculated by mass, the invention comprises: 8-12 parts of sweet potato leaves, 48-52 parts of angelica dahurica, 48-52 parts of bletilla striata, 13-17 parts of sodium sulfate, 28-32 parts of moutan bark, 28-32 parts of safflower, 48-52 parts of selfheal, 18-22 parts of fenugreek, 28-32 parts of atractylodes macrocephala, 28-32 parts of poria, 28-32 parts of white aconite and 28-32 parts of pearl powder.

4. The sweet potato leaf whitening cream according to claim 1, characterized in that: It also includes 8 to 12 parts of mint and 0.5 to 2 parts of borneol.

5. The sweet potato leaf whitening cream according to claim 1, characterized in that: The sweet potato leaves are freeze-dried sweet potato leaves powder, and the preparation method of the freeze-dried sweet potato leaves powder comprises: The sweet potato leaves are crushed, added with water, mixed, refluxed for extraction, filtered, the filtrate is collected, concentrated, and then freeze-dried to obtain the sweet potato leaf freeze-dried powder; The solid-liquid ratio of the sweet potato leaves to water is 1:6-15 g / mL; the temperature of the reflux extraction is 70-75° C., the extraction time is 1-2 hours, and the reflux extraction is repeated 2-3 times.

6. The sweet potato leaf whitening cream according to claim 1, characterized in that: The preparation method of the traditional Chinese medicine whitening active ingredient comprises: (1) crushing sweet potato leaves, adding water to mix, refluxing extraction, filtering, collecting the filtrate, concentrating, and then freeze-drying to obtain sweet potato leaf freeze-dried powder; The mass volume ratio of the sweet potato leaves to water is 1:6-15 g / mL; the temperature of the reflux extraction is 70-75° C., the extraction time is 1-2 hours, and the reflux extraction is repeated 2-3 times; (2) taking angelica dahurica, bletilla striata, mirabilite, moutan bark, safflower, selfheal, fenugreek, atractylodes macrocephala, poria and white aconite, obtaining the medicinal materials, crushing them, adding water for soaking and extraction, then decocting, filtering, taking the filtrate, and concentrating it to 4% to 6% of the total volume of the original filtrate to obtain the extracted Chinese medicinal liquid; The weight ratio of the medicinal material to water is 1:10-15; the soaking extraction time is 2-3 hours; (3) Adding the freeze-dried powder of sweet potato leaves and pearl powder to the extracted Chinese medicine solution and stirring, thereby obtaining the whitening active ingredient of Chinese medicine.

7. The method for preparing the sweet potato leaf whitening cream according to any one of claims 1 to 6, characterized in that: The steps include: Formula S1: The amount of the whitening active ingredient of traditional Chinese medicine added is 3-8% by mass percentage; S2 mixing: mix the moisturizer and water, stir, add the whitening active ingredient of traditional Chinese medicine, continue stirring, and obtain the sweet potato leaf whitening cream.

8. Use of the sweet potato leaf whitening cream according to any one of claims 1 to 6 in inhibiting tyrosinase activity.

9. Use of the sweet potato leaf whitening cream according to any one of claims 1 to 6 in whitening and repairing after sun exposure.

10. The use according to claim 9, characterized in that: The sweet potato leaf whitening cream is used in inhibiting melanin deposition and / or increasing the SOD enzyme content in skin tissue after sun exposure.