Bacteriostatic spray and preparation method and application thereof

Abstract

The invention relates to the technical field of bacteriostatic spray, and provides bacteriostatic spray as well as a preparation method and application thereof. The bacteriostatic spray is prepared from the following raw materials: a rhodiola rosea extracting solution, a saffron crocus extracting solution, a lamiophlomis rotata extracting solution, a rhizoma drynariae extracting solution, an angelica sinensis extracting solution, a radix clematidis extracting solution, a ligusticum wallichii extracting solution, a garden balsam stem extracting solution, a lycopodium clavatum extracting solution, a radix salviae miltiorrhizae extracting solution, a cassia twig extracting solution, turpentine, borneol, trichloro hydroxydiphenyl ether, a lavender extracting solution and auxiliary liquid. According to the antibacterial spray disclosed by the invention, various natural active substance components can be fused, so that the antibacterial spray has the effects of inhibiting bacteria, preserving moisture, protecting skin, resisting inflammation and soothing the nerves, is rich in fresh, cool and fragrant and sweet smell, is mild to skin, is safe and non-irritant, and does not harm human skin, so that the antibacterial spray has more effects and a better use effect; various requirements of people can be met, and the product capacity is better.

Description

Technical Field

[0001] The present invention relates to the technical field of antibacterial spray, in particular to an antibacterial spray. The present invention also relates to a preparation method and application of the antibacterial spray. Background Art

[0002] Currently, with the public's awareness of hygienic environments and healthy disinfection significantly increasing, the demand for medical protection and disinfection products continues to grow. Demand for disinfectant products has skyrocketed across regions, driving the continued expansion of the disinfection product market. Among them, disinfectant and antibacterial sprays, indispensable in daily life, have become a must-have for people at home and on the go. However, while there are a wide variety of antibacterial sprays on the market, most have a single function, limited to disinfection and antibacterial treatment, which is not conducive to meeting people's diverse needs. Therefore, the development of an antibacterial spray product that incorporates active ingredients from traditional Chinese medicine and possesses multiple benefits has become a critical need that needs to be addressed in the disinfection field. Summary of the Invention

[0003] In view of this, the present invention aims to provide an antibacterial spray with antibacterial, moisturizing, skin care, anti-inflammatory and tranquilizing effects.

[0004] To achieve the above object, the technical solution of the present invention is achieved as follows:

[0005] Disclosed is an antibacterial spray, the raw materials of which include rhodiola rosea extract, saffron extract, radix pubescens extract, rhizoma scopariae extract, angelica extract, clematis root extract, ligusticum chuanxiong extract, herba schizonepetae extract, herba schizonepetae extract, salvia miltiorrhizae extract, cassia twig extract, turpentine, borneol, trichlorohydroxydiphenyl ether, lavender extract and auxiliary liquid.

[0006] Furthermore, the raw materials of the antibacterial spray are respectively as follows by weight percentage: 0.01%-0.5% of Rhodiola rosea extract, 0.01%-0.4% of saffron extract, 0.01%-0.8% of Duyiwei extract, 0.01%-0.3% of Gubuxiu extract, 0.01%-0.3% of Angelica sinensis extract, 0.01%-0.3% of Clematidis extract, 0.01%-0.3% of Chuanxiong extract, 0.01%-0.3% of Polygonum multiflorum extract, 0.01%-0.3% of Lycopodiella vulgaris extract, 0.01%-0.3% of Salvia miltiorrhiza extract, 0.01%-0.3% of cassia twig extract, 0.1%-0.3% of turpentine, 0.3%-0.6% of borneol, 0.1%-0.25% of trichlorohydroxydiphenyl ether, 0.3%-0.5% of lavender extract, and the rest are auxiliary liquids.

[0007] Furthermore, the auxiliary liquid includes ethanol and purified water, and by weight percentage: ethanol 25%-40%, and the rest is purified water.

[0008] The main components of Rhodiola rosea are salidroside, tyrosol, and gallic acid, which have antioxidant and anti-inflammatory effects and prevent skin diseases. The main components of saffron are flavonoids and their glycoside compounds, which have anti-inflammatory, antioxidant, anti-allergic, and antiviral effects. The main components of Lamiophlomis odorifera are luteolin and total iridoid ether glycosides, which have hemostatic, analgesic, and anti-inflammatory effects. Other medicinal extracts also have anti-inflammatory and analgesic, antibacterial, and antimicrobial effects, as well as the effects of dispelling wind and dampness, dredge meridians and relieve pain, and detoxify and reduce swelling. These medicinal extracts are widely used in daily necessities, the food industry, and the medical field.

[0009] The antibacterial spray described in the present invention can integrate multiple natural active substance components by fusing multiple traditional Chinese medicine extracts such as Rhodiola rosea, saffron, Lamiophloea chinensis and Gastrodia elata. The components of each traditional Chinese medicine extract solution have clear efficacy, are safe and have no toxic side effects, and can fully utilize natural Chinese herbal medicines, which is beneficial to improving resource utilization and enhancing the product's antibacterial, anti-inflammatory, skin care and calming effects, thereby meeting people's various needs for sprays and having better product strength.

[0010] Secondly, the present invention primarily uses a traditional Chinese medicine extract solution, ethanol, and water as its solvent system. No chemical flavoring is required; only lavender extract and borneol are added. This results in a product with a fresh, cool, and sweet aroma that is gentle on the skin, safe, and non-irritating. Furthermore, the required preparation process is mature, the equipment investment is minimal, and the overall system is stable, enabling rapid mass production.

[0011] In addition, the present invention also provides a method for preparing the antibacterial spray as described above, comprising:

[0012] Mixing Rhodiola rosea extract, saffron extract, Lamiophlomis pubescens extract, Gastrodia elata extract, Angelica sinensis extract, Clemati radix extract, Chuanxiong extract, Herba schizonepetae extract, Herba Lycopodii extract, Salvia miltiorrhizae extract, and Cinnamomum cassiae extract to obtain a mixed solution A;

[0013] Mix turpentine, borneol, trichlorohydroxydiphenyl ether and lavender extract, and stir to obtain a mixed solution B;

[0014] The obtained mixed solution A and auxiliary liquid are mixed and stirred, and then the mixed solution B is added and stirred. After standing, an antibacterial spray product is obtained.

[0015] Furthermore, the turpentine oil, the borneol, the trichlorohydroxy diphenyl ether and the lavender extract are mixed according to weight percentage and stirred to obtain the mixed solution B, which includes: mixing the turpentine oil, the borneol, the trichlorohydroxy diphenyl ether and the lavender extract and stirring at 20-26°C to obtain the mixed solution B.

[0016] Furthermore, the stirring time is between 30-45 minutes.

[0017] Furthermore, the obtained mixed liquid A and the auxiliary liquid are mixed and stirred, and then the mixed liquid B is added and stirred, and the mixture is allowed to stand to obtain the antibacterial spray product, including: mixing the obtained mixed liquid A and the auxiliary liquid, stirring at 20-26°C, and then slowly adding the mixed liquid B and stirring, and the mixture is allowed to stand to obtain the antibacterial spray product; and / or, mixing purified water and ethanol to obtain the auxiliary liquid.

[0018] Furthermore, the duration of the two stirring times is between 30-60 minutes; and / or the standing time is between 1-36 hours.

[0019] The preparation method of the antibacterial spray described in the present invention is not only simple and easy to operate, but also greatly shortens the preparation time, which is conducive to improving the preparation efficiency. It also does not require the use of high-end production equipment such as homogenizers, and can achieve sufficient and uniform mixing, which is conducive to reducing costs. At the same time, it can also well protect the natural medicinal active ingredients in Chinese herbal medicines, so that the prepared antibacterial spray product has multiple functions such as antibacterial, moisturizing, anti-inflammatory and skin care, and has no organic solvent residue, is green and additive-free.

[0020] In addition, the present invention also provides the use of the antibacterial spray in inhibiting Staphylococcus aureus, Escherichia coli or Candida albicans, and the use of the antibacterial spray in skin and mucous membrane hygiene.

[0021] The antibacterial spray of the present invention is applied to the hygiene of skin and mucous membranes, has no irritation to the skin, has good system stability, and has multiple functions such as antibacterial, moisturizing, anti-inflammatory and skin care.

[0022] The antibacterial spray of the present invention complies with the requirements of the "Technical Specifications for Disinfection", can inhibit and disinfect Staphylococcus aureus, Escherichia coli and Candida albicans, and can meet people's daily disinfection and sterilization needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The accompanying drawings, which constitute part of the present invention, are provided to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are provided to explain the present invention and do not constitute an undue limitation of the present invention. In the accompanying drawings:

[0024] Figure 1 The present invention is a flowchart of a method for preparing the antibacterial spray according to an embodiment of the present invention. DETAILED DESCRIPTION

[0025] It should be noted that, in the absence of conflict, the embodiments of the present invention and the features in the embodiments may be combined with each other.

[0026] The present invention will be described in detail below with reference to the accompanying drawings and in conjunction with embodiments.

[0027] This embodiment relates to an antibacterial spray, which can not only be used for disinfection and antibacterial, but also can meet people's needs such as skin care, moisturizing, anti-inflammatory and calming, and has better use effect and product strength.

[0028] In terms of overall design, the antibacterial spray of this embodiment includes raw materials such as Rhodiola rosea extract, saffron extract, Duyiwei extract, Gubuxiu extract, Angelica sinensis extract, Clematis chinensis extract, Chuanxiong extract, Polygonum multiflorum extract, Herba Lycopodii extract, Salvia miltiorrhiza extract, cinnamon twig extract, turpentine, borneol, trichlorohydroxydiphenyl ether, lavender extract and auxiliary liquid.

[0029] At this time, as designed above, a variety of Chinese herbal extracts such as Rhodiola rosea, saffron, Duyiwei, Gubuxiu, Angelica sinensis, Clematis chinensis, Chuanxiong, Radix angelicae dahuricae, Herba Lycopodii, Salvia miltiorrhizae and cinnamon twigs were selected, which contain organic compounds such as alcohols, aldehydes, acids, phenols, lipids, ketones and terpenes. These compounds work together to achieve a synergistic effect, enhance the inhibitory and bactericidal activity against Staphylococcus aureus, Escherichia coli, Candida albicans, etc., enhance the calming effect of human skin, and achieve the effects of moisturizing, skin care, tranquilizing the nerves, and anti-inflammatory.

[0030] Specifically, alcohols in sprays may have antibacterial, anti-inflammatory, immune-enhancing and spirit-boosting effects. They are gentle on the skin, non-toxic, and can help improve the skin environment and promote blood circulation. Aldehydes have moisturizing, antibacterial and skin hydration effects. They can also metabolize waste toxic substances in the human body and balance emotions. However, it should be noted that since some aldehydes may be irritating to the skin, the dosage needs to be controlled when used. Esters can have antispasmodic, anti-inflammatory, soothing effects on the nervous system and stimulating the regeneration of skin cells. They can also increase the amount of collagen, soften scar tissue, and have wrinkle removal and anti-aging effects. In addition, esters are one of the safest and mildest chemical ingredients and will not irritate the skin.

[0031] Phenols can have whitening, strong stimulation of female hormones and antibacterial effects. However, it should be noted that they are irritating to the skin and need to be diluted and the dosage controlled when used to avoid adverse reactions caused by excessive use. Lipids (or esters) mainly play the role of skin care, anti-aging and soothing the nervous system. An appropriate amount of ketones can have therapeutic effects such as anti-inflammatory, anti-allergic and stabilizing cell membranes. Terpenes are important components of plant essential oils, including monoterpenes and sesquiterpenes. They may have multiple effects in sprays, such as detoxification, antibacterial, expectorant and digestion promotion. Sesquiterpenes, in particular, can directly penetrate the blood-brain barrier in a specific way, providing new therapeutic ideas for the treatment of brain diseases.

[0032] It's also worth mentioning that the main components of Rhodiola rosea extract, including salidroside, tyrosol, and gallic acid, have antioxidant and anti-inflammatory effects and are also protective against skin diseases. The main components of saffron include flavonoids and their glycosides, which have anti-inflammatory, antioxidant, anti-allergic, and antiviral properties. The main components of Lamiophlomis odorifera include luteolin and total iridoid glycosides, which have hemostatic, analgesic, and anti-inflammatory effects. The main active ingredient in Drynaria fortunei extract is naringin, and it also contains methyl eugenol, neoeugenol, protocatechuic acid, and flavonoids. It has multiple benefits, including anti-inflammatory, anti-allergic, anti-aging, and whitening effects. The main components of Angelica sinensis extract include volatile oils, flavonoids, sugars, and organic acids, and have multiple benefits, including nourishing and activating blood circulation, regulating menstruation and relieving pain, moistening the intestines and promoting bowel movements, and nourishing and calming the mind.

[0033] The extract of Clematis chinensis contains various ingredients such as anemonin, anemonial lactone, sterols, sugars, saponins, etc., and has multiple pharmacological effects such as analgesia, anti-inflammatory, anti-diuretic, choleretic, and antibacterial. The extract of Chuanxiong mainly contains active ingredients such as phthalide, alkaloids, phenolic acid, polysaccharides, chuanxiongzine, ferulic acid, etc., and has multiple effects such as anti-inflammatory, antioxidant and antibacterial effects, whitening and moisturizing the skin. The main ingredients of the extract of Herba Lycopodii include extracts of Chinese medicinal materials such as Artemisia argyi, Panax notoginseng, safflower, Herba Lycopodii, Herba Lycopodii (Shujincao), Angelica dahurica, Notopterygium wilfordii, Ligusticum chuanxiong, Caulis Sanguisorbae and Angelica sinensis, and have multiple effects such as promoting blood circulation, unblocking meridians, dispelling cold, removing dampness, relieving pain, reducing swelling, and promoting wound healing. The extract of Herba Lycopodii mainly contains the active ingredients of Herba Lycopodii, and has multiple effects such as anti-inflammatory, antipyretic, analgesic, dispelling wind and dampness, relaxing muscles and activating meridians, dispelling cold and relieving pain.

[0034] Danshen extract mainly includes tanshinone, salvianolic acid, cryptotanshinone, danshensu, rosmarinic acid, and other ingredients, and has multiple benefits, including promoting blood circulation, calming the nerves, anti-oxidation, antibacterial and anti-inflammatory effects, and promoting blood circulation. Cinnamon twig extract mainly contains volatile oils (such as cinnamaldehyde, cinnamic acid, cinnamyl acetate, and phenylpropyl acetate), phenols, organic acids, polysaccharides, glycosides, coumarins, and tannins, and has antipyretic and cooling, antibacterial and anti-inflammatory, digestive enhancement, analgesic and anticonvulsant, and anti-aging effects.

[0035] Turpentine has antibacterial and antifungal properties, promotes wound healing, relieves muscle pain, and improves itchy skin. Turpentine contains several active ingredients, such as eucalyptol and α-pinene, which can interfere with bacterial cell wall synthesis or destroy bacterial cell membrane structure, thereby inhibiting the growth and reproduction of bacteria and fungi. Trichlorohydroxydiphenyl ether can also have antibacterial, disinfectant, and deodorizing effects.

[0036] The main effects of borneol in the spray include clearing away heat and detoxifying, reducing swelling and relieving pain, preventing decay and promoting tissue regeneration, and refreshing the mind. Borneol is slightly cold in nature, pungent and bitter in taste, and enters the heart, spleen, and lung meridians. It has the effect of clearing away heat and detoxifying, and can relieve symptoms such as sores, sore throat, and mouth sores caused by heat toxins. In addition, borneol also has the effect of reducing swelling and relieving pain, and can be used to relieve local redness, swelling, pain, and other discomfort symptoms caused by traumatic injuries. The antiseptic and tissue regeneration effects of borneol make it effective in treating sores, sores, burns, and scalds. At the same time, borneol can also refresh the mind and be used to relieve symptoms such as coma caused by febrile diseases, phlegm caused by stroke, and sudden fainting caused by qi stagnation. Other medicinal extracts also have anti-inflammatory and analgesic effects, antibacterial and antimicrobial effects, and can dispel wind and dampness, promote blood circulation and relieve pain, and detoxify and reduce swelling. These medicinal extracts are widely used in daily necessities, the food industry, and the medical field.

[0037] The main effects of lavender extract in sprays include soothing and relaxing the body and mind, promoting sleep, and improving sleep quality. Lavender extract has a unique fragrance that is believed to have a soothing and relaxing effect, helping people relieve tension and anxiety, thereby helping to fall asleep faster and improve sleep quality.

[0038] Therefore, the antibacterial spray of this embodiment includes the above-mentioned raw materials, which can make the raw materials promote each other and synergize with each other, and has the effect of "1+1>2", and has the effects of antibacterial, moisturizing, skin care, anti-inflammatory and calming, thereby improving the single function of the antibacterial spray, so as to meet people's other needs such as anti-inflammatory and moisturizing in addition to disinfection and antibacterial. It can be used to inhibit Staphylococcus aureus, Escherichia coli or Candida albicans, and can also be used in scenarios such as skin and mucous membrane hygiene.

[0039] The antibacterial spray of this embodiment is able to integrate multiple natural active substance components by fusing multiple Chinese herbal medicine extracts such as Rhodiola rosea, saffron, Dumifug and Gastrodia elata. The components of each Chinese herbal medicine extract solution have clear efficacy, are safe and have no toxic side effects, and can fully utilize natural Chinese herbal medicines, which is beneficial to improving resource utilization and enhancing the product's antibacterial, anti-inflammatory, moisturizing, skin care and calming effects.

[0040] Secondly, the present invention primarily uses a traditional Chinese medicine extract solution, ethanol, and water as its solvent system. No chemical flavoring is required; only lavender extract and borneol are added. This results in a product with a fresh, cool, and sweet aroma that is gentle on the skin, safe, and non-irritating. Furthermore, the required preparation process is mature, the equipment investment is minimal, and the overall system is stable, enabling rapid mass production.

[0041] In specific implementation, as a preferred embodiment, the raw materials of the antibacterial spray of this embodiment are respectively as follows by weight percentage:

[0042] Rhodiola rosea extract 0.01%-0.5%, saffron extract 0.01%-0.4%, Duyiwei extract 0.01%-0.8%, Gubuxiu extract 0.01%-0.3%, angelica extract 0.01%-0.3%, Clematidis extract 0.01%-0.3%, Chuanxiong extract 0.01%-0.3%, pelargonium oleifera extract 0.01%-0.3%, Lycopodiella vulgaris extract 0.01%-0.3%, Salvia miltiorrhiza extract 0.01%-0.3%, cassia twig extract 0.01%-0.3%, turpentine 0.1%-0.3%, borneol 0.3%-0.6%, trichlorohydroxydiphenyl ether 0.1%-0.25%, lavender extract 0.3%-0.5%, and the rest are auxiliary liquids. Here, the auxiliary liquid preferably includes ethanol and purified water, and by weight percentage: ethanol 25%-40% and the rest is purified water.

[0043] Among them, turpentine can be specifically used at 0.2%, borneol can be specifically used at 0.5%, trichlorohydroxydiphenyl ether can be specifically used at 0.17%, lavender extract can be specifically used at 0.4%, and ethanol can be specifically used at 30%, so as to facilitate uniform mixing of the raw materials and make the antibacterial spray have a fresh, cool and sweet smell.

[0044] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above simultaneously. The antibacterial spray of this embodiment is primarily intended to address the problem that existing antibacterial sprays have a single function, which is only capable of disinfection and antibacterial. Some existing antibacterial sprays even have odor irritation, nerve stimulation, and are unfriendly to the skin, which is not conducive to meeting people's needs for skin care, calming the nerves, etc. in addition to disinfection and antibacterial.

[0045] In addition, the present invention also provides a method for preparing an antibacterial spray, such as Figure 1 As shown, the preparation method comprises:

[0046] S1. Mixing Rhodiola rosea extract, Saffron extract, Lamiophlomis pubescens extract, Boneless Boneless Extract, Angelica sinensis extract, Clematidis extract, Chuanxiong extract, Herba schizonepetae extract, Herba Lycopodii extract, Salvia miltiorrhizae extract, and Cinnamomum cassiae extract according to weight percentage to obtain a mixed solution A;

[0047] S2, mixing turpentine, borneol, trichlorohydroxydiphenyl ether and lavender extract according to weight percentage, stirring to obtain a mixed solution B;

[0048] S3. Mix the obtained mixed solution A and auxiliary liquid, stir them, then add mixed solution B and stir them. After standing, an antibacterial spray product is obtained.

[0049] As a preferred embodiment, in S2, turpentine, borneol, trichlorohydroxy diphenyl ether, and lavender extract are mixed according to weight percentage (i.e., the weight percentage of the separator materials mentioned above), and stirred at room temperature (20-26° C.) to obtain a mixed solution B. The stirring time can preferably be between 30 and 45 minutes, and can specifically be, for example, 35 minutes, 40 minutes, or 45 minutes, so that the turpentine, borneol, trichlorohydroxy diphenyl ether, and lavender extract are fully mixed.

[0050] As a preferred embodiment, purified water and ethanol are mixed according to weight percentage to obtain an auxiliary liquid. Simultaneously, in S3, the obtained mixed liquid A and the auxiliary liquid are mixed, stirred at room temperature (20-26°C), and then mixed liquid B is slowly added and stirred. After standing, the mixture is obtained as an antibacterial spray product. The room temperature can be specifically 23°C or 25°C to facilitate mixing of the raw materials.

[0051] During specific implementation, in S3, the duration of the two stirrings can be preferably set between 30-60min, and for example, specifically can be 40min, 45min or 55min, etc., so that after mixed solution A and mixed solution C are fully mixed, they are fully mixed with mixed solution B. In addition, the standing time is preferably set between 1-36h, and for example, specifically can be 3h, 8h, 12h, 24h or 30h, etc., so that various natural active ingredients are fully mixed and uniform, and the stability and use effect of the spray are guaranteed. Of course, standing still also helps to observe whether unstable phenomena such as stratification and precipitation occur in the mixed liquid, so that they can be handled in time. If these problems occur in the spray after standing still, it may mean that the formula needs to be adjusted or the production process needs to be improved.

[0052] The preparation method of the antibacterial spray of this embodiment is not only simple and easy to operate, but also greatly shortens the preparation time, which is conducive to improving the preparation efficiency. It also does not require the use of high-end production equipment such as homogenizers to achieve sufficient and uniform mixing, which is conducive to reducing costs. At the same time, it can also provide good protection for the natural medicinal active ingredients in Chinese herbal medicines, so that the prepared antibacterial spray product has multiple functions such as antibacterial, moisturizing, anti-inflammatory and skin care, and has no organic solvent residue, is green and additive-free.

[0053] Here, the present invention is further described with reference to specific embodiments:

[0054] Example 1

[0055] An antibacterial spray, wherein the raw materials are respectively as follows by weight percentage:

[0056] Rhodiola rosea extract 0.01%, saffron extract 0.01%, Duyiwei extract 0.01%, Gubuxiu extract 0.01%, Angelica sinensis extract 0.01%, Clematidis extract 0.01%, Chuanxiong extract 0.01%, Polygonum multiflorum extract 0.01%, Lycopodiella vulgaris extract 0.01%, Salvia miltiorrhiza extract 0.01%, Cassia twig extract 0.01%, turpentine oil 0.2%, borneol 0.5%, trichloroethylene diphenyl ether 0.17%, lavender extract 0.4%, ethanol 30%, purified water 68.62%.

[0057] The preparation method of the antibacterial spray comprises:

[0058] Fully dissolve 0.01% Rhodiola rosea extract, 0.01% saffron extract, 0.01% Lamiophlomis extract, 0.01% Boneless Boneless Extract, 0.01% Angelica sinensis extract, 0.01% Clematidis extract, 0.01% Chuanxiong extract, 0.01% Herba Polygoni Multiflori extract, 0.01% Herba Lycopodii extract, 0.01% Salvia miltiorrhiza extract, and 0.01% cinnamon twig extract to prepare a mixed solution A.

[0059] Subsequently, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxydiphenyl ether, and 0.4% lavender extract are mixed and stirred at room temperature for 30-45 minutes to prepare mixed solution B. Simultaneously, 68.62% purified water and 30% ethanol are mixed and fully dissolved to prepare an auxiliary liquid. Finally, the obtained mixed solution A and the auxiliary liquid are mixed with each other and stirred at room temperature for 30-60 minutes. The obtained mixed solution B is then slowly added to the above solution, stirred for 30-60 minutes, and allowed to stand for 1-36 hours to obtain the antibacterial spray product.

[0060] Example 2

[0061] An antibacterial spray, wherein the raw materials are respectively as follows by weight percentage:

[0062] Rhodiola rosea extract 0.1%, saffron extract 0.1%, Duyiwei extract 0.1%, Gubuxiu extract 0.1%, Angelica sinensis extract 0.1%, Clematidis extract 0.1%, Chuanxiong extract 0.1%, Polygonum multiflorum extract 0.1%, Lycopodiella vulgaris extract 0.1%, Salvia miltiorrhiza extract 0.1%, cinnamon twig extract 0.1%, turpentine oil 0.2%, borneol 0.5%, trichloroethylene diphenyl ether 0.17%, lavender extract 0.4%, ethanol 30%, purified water 68.18%.

[0063] The preparation method of the antibacterial spray comprises:

[0064] Fully dissolve 0.1% Rhodiola rosea extract, 0.1% Saffron extract, 0.1% Lamiophlomis extract, 0.1% Boneless Plant extract, 0.1% Angelica sinensis extract, 0.1% Clematidis extract, 0.1% Chuanxiong extract, 0.1% Herba Polygoni Multiflori extract, 0.1% Herba Lycopodii extract, 0.1% Salvia miltiorrhiza extract, and 0.1% Cinnamomum cassia extract to prepare a mixed solution A.

[0065] Subsequently, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxydiphenyl ether, and 0.4% lavender extract are mixed and stirred at room temperature for 30-45 minutes to prepare mixed solution B. Simultaneously, 68.18% purified water and 30% ethanol are mixed and fully dissolved to prepare an auxiliary liquid. Finally, the obtained mixed solution A and the auxiliary liquid are mixed and stirred at room temperature for 30-60 minutes. The obtained mixed solution B is then slowly added to the above solution, stirred for 30-60 minutes, and allowed to stand for 1-36 hours to obtain the antibacterial spray product.

[0066] Example 3

[0067] An antibacterial spray, wherein the raw materials are respectively as follows by weight percentage:

[0068] Rhodiola rosea extract 0.2%, saffron extract 0.2%, Duyiwei extract 0.2%, Gubuxiu extract 0.2%, angelica extract 0.2%, Clematidis extract 0.2%, Chuanxiong extract 0.2%, pelargonium oleifera extract 0.2%, Lycopodiella vulgaris extract 0.2%, Salvia miltiorrhiza extract 0.2%, cassia twig extract 0.2%, turpentine oil 0.2%, borneol 0.5%, trichloroethylene ether 0.17%, lavender extract 0.4%, ethanol 30%, purified water 66.53%.

[0069] The preparation method of the antibacterial spray comprises:

[0070] Fully dissolve 0.2% of Rhodiola rosea extract, 0.2% of Saffron extract, 0.2% of Lamiophlomis pubescens extract, 0.2% of Boneless Plant extract, 0.2% of Angelica sinensis extract, 0.2% of Clematidis extract, 0.2% of Chuanxiong extract, 0.2% of Herba Polygoni Multiflori extract, 0.2% of Herba Lycopodii Herba, 0.2% of Herba Salviae Miltiorrhizae extract, and 0.2% of Cinnamomum cassiae extract to prepare a mixed solution A.

[0071] Subsequently, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxydiphenyl ether, and 0.4% lavender extract are mixed and stirred at room temperature for 30-45 minutes to prepare mixed solution B. Simultaneously, 66.53% purified water and 30% ethanol are mixed and fully dissolved to prepare an auxiliary liquid. Finally, the obtained mixed solution A and the auxiliary liquid are mixed and stirred at room temperature for 30-60 minutes. The obtained mixed solution B is then slowly added to the above solution, stirred for 30-60 minutes, and allowed to stand for 1-36 hours to obtain the antibacterial spray product.

[0072] Example 4

[0073] An antibacterial spray, wherein the raw materials are respectively as follows by weight percentage:

[0074] Rhodiola rosea extract 0.3%, saffron extract 0.3%, Duyiwei extract 0.3%, Gubuxiu extract 0.3%, Angelica sinensis extract 0.3%, Clematidis extract 0.3%, Chuanxiong extract 0.3%, Polygonum multiflorum extract 0.3%, Lycopodiella vulgaris extract 0.3%, Salvia miltiorrhiza extract 0.3%, cinnamon twig extract 0.3%, turpentine oil 0.2%, borneol 0.5%, trichloroethylene diphenyl ether 0.17%, lavender extract 0.4%, ethanol 30%, purified water 65.43%.

[0075] The preparation method of the antibacterial spray comprises:

[0076] Fully dissolve 0.3% of Rhodiola rosea extract, 0.3% of saffron extract, 0.3% of Lamiophlomis pubescens extract, 0.3% of Gastrodia elata extract, 0.3% of Angelica sinensis extract, 0.3% of Clematidis extract, 0.3% of Chuanxiong extract, 0.3% of Herba Polygoni Multiflori extract, 0.3% of Herba Lycopodii Herba, 0.3% of Herba Salviae Miltiorrhizae extract, and 0.3% of Cinnamomum cassiae extract to prepare a mixed solution A.

[0077] Subsequently, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxydiphenyl ether, and 0.4% lavender extract are mixed and stirred at room temperature for 30-45 minutes to prepare mixed solution B. Simultaneously, 65.43% purified water and 30% ethanol are mixed and fully dissolved to prepare an auxiliary liquid. Finally, the obtained mixed solution A and the auxiliary liquid are mixed and stirred at room temperature for 30-60 minutes. The obtained mixed solution B is then slowly added to the above solution, stirred for 30-60 minutes, and allowed to stand for 1-36 hours to obtain the antibacterial spray product.

[0078] Example 5

[0079] An antibacterial spray, wherein the raw materials are respectively as follows by weight percentage:

[0080] Rhodiola rosea extract 0.5%, saffron extract 0.4%, Duyiwei extract 0.8%, Gubuxiu extract 0.3%, angelica extract 0.3%, Clematidis extract 0.3%, Chuanxiong extract 0.3%, peony root extract 0.3%, smilax china extract 0.3%, smilax china extract 0.3%, salvia miltiorrhiza extract 0.3%, cassia twig extract 0.3%, turpentine 0.2%, borneol 0.5%, trichloroethylene ether 0.17%, lavender extract 0.4%, ethanol 30%, purified water 64.63%.

[0081] The preparation method of the antibacterial spray comprises:

[0082] Fully dissolve 0.5% of Rhodiola rosea extract, 0.4% of Saffron extract, 0.8% of Lamiophlomis chinensis extract, 0.3% of Boneless Plant extract, 0.3% of Angelica sinensis extract, 0.3% of Clematidis extract, 0.3% of Chuanxiong extract, 0.3% of Herba Polygoni Multiflori extract, 0.3% of Herba Lycopodii Herba, 0.3% of Herba Salviae Miltiorrhizae extract, and 0.3% of Cinnamomum cassiae extract to prepare a mixed solution A.

[0083] Subsequently, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxydiphenyl ether, and 0.4% lavender extract are mixed and stirred at room temperature for 30-45 minutes to prepare mixed solution B. Simultaneously, 64.63% purified water and 30% ethanol are mixed to fully dissolve each other to prepare an auxiliary liquid. Finally, the obtained mixed solution A and the auxiliary liquid are mixed and stirred at room temperature for 30-60 minutes. The obtained mixed solution B is then slowly added to the above solution, stirred for 30-60 minutes, and allowed to stand for 1-36 hours to obtain the antibacterial spray product.

[0084] Antibacterial test of antibacterial spray

[0085] Experimental methods:

[0086] Staphylococcus aureus, Escherichia coli, and Candida albicans were cultured in culture medium and used in the experiment.

[0087] The antibacterial liquid samples of Examples 1-5 were added to the sterilized PBS buffer solution for 2 min, 5 min, 10 min, and 20 min, respectively. The microbial culture solution was inoculated and then cultured at a suitable temperature.

[0088] In addition, a blank experimental liquid was used as a control group. The control group was inoculated with microorganisms in PBS buffer and no antibacterial substances were added. Since no antibacterial substances were added, the antibacterial rate of the control group can be regarded as 0, so it is not reflected in Tables 1 to 3 below.

[0089] Table 1: Staphylococcus aureus antibacterial test results

[0090]

[0091] Table 2: E. coli antibacterial test results

[0092]

[0093] Table 3: Candida albicans antibacterial test results

[0094]

[0095]

[0096] From the above data, the Chinese medicine extract of this embodiment, that is, the antibacterial spray, has antibacterial and anti-inflammatory effects. When the Chinese medicine extract is compounded in a certain proportion, the antibacterial effect on Staphylococcus aureus, Escherichia coli and Candida albicans can be improved. In particular, the raw materials in the antibacterial spray are respectively "Rhodiola rosea extract 0.5%, saffron extract 0.4%, Duyiwei extract 0.8%, bone succulent extract 0.3%, angelica extract 0.3%, and clematis extract 0.3% by weight. The antibacterial spray of this example achieved an antibacterial effect of 99.99% against Staphylococcus aureus, Escherichia coli, and Candida albicans within 2 minutes, with a more pronounced antibacterial effect. The composition of the spray was 0.3% Chuanxiong extract, 0.3% Herba Schizonepetae extract, 0.3% Salvia miltiorrhiza extract, 0.3% Cinnamomum cassia twig extract, 0.2% turpentine, 0.5% borneol, 0.17% trichlorohydroxydiphenyl ether, 0.4% lavender extract, 30% ethanol, and 64.63% purified water.

[0097] Of course, it's worth mentioning that PBS, or Phosphate Buffered Saline (PBS), is a widely used buffer solution in biochemical research. The main components of PBS include Na2HPO4, KH2PO4, NaCl, and KCl, which work together to maintain a stable pH in the solution. Because Na2HPO4 and KH2PO4 exhibit secondary dissociation, PBS has a wide pH range, adapting to diverse experimental needs. Furthermore, the addition of NaCl and KCl increases the salt ion concentration, further stabilizing the solution environment. PBS has numerous applications in biological research. Because it is isotonic and non-toxic to cells, it is often used to dilute substances, clean cell containers, and wash cells and tissues. Furthermore, because its osmotic pressure and ion concentration are generally similar to human pH, PBS is often used to simulate the human body environment for related biochemical experiments.

[0098] Skin test of antibacterial spray:

[0099] Experimental methods:

[0100] Three male white rabbits were selected. 24 hours before the experiment, the hair on both sides of the spine of the animals was removed. The hair removal area was 3 cm × 3 cm. The epidermis should not be damaged.

[0101] Take 0.5 mL of the antibacterial liquid in Example 5 as a sample and apply it directly to the hairless skin on one side of the animal (the other side serves as a blank control). The application area is 2.5 cm × 2.5 cm. 4 hours after application, wash with water or a suitable non-irritating solvent to remove the residue.

[0102] Apply once a day for 14 consecutive days. Observe the results 24 hours after each application and score according to the skin irritation scoring standard of the "Disinfection Technical Specifications". No skin irritation reaction occurred after 14 consecutive days of application. The test scores are shown in Table 4 below:

[0103] Table 4 Skin test results

[0104]

[0105] Based on the data in the table above and in accordance with the skin irritation intensity grading evaluation criteria in the "Technical Specifications for Disinfection," the antibacterial liquid (antibacterial spray) of this example was found to be non-irritating in multiple intact rabbit skin irritation tests. This means that the antibacterial liquid (antibacterial spray) of this application is mild, safe, and non-irritating to the skin, and does not harm human skin.

[0106] At the same time, the antibacterial liquid (antibacterial spray) of this embodiment can be judged by smell to be rich in fresh, cool and sweet smell, and in the case of skin test, no abnormal reaction of rabbits to the smell of the antibacterial liquid (antibacterial spray) was observed, which shows that the smell of the antibacterial liquid (antibacterial spray) of this embodiment is more easily accepted by people.

[0107] To this end, the antibacterial spray of this embodiment can be used to inhibit Staphylococcus aureus, Escherichia coli or Candida albicans, and can also be used for skin and mucous membrane hygiene. It has a wide range of usage scenarios. It not only has antibacterial, anti-inflammatory and calming effects, but is also gentle on the skin, safe and non-irritating, does not harm human skin, is beneficial for moisturizing and skin care, and at the same time, is rich in fresh, cool and sweet smells, and has better use effects.

[0108] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. An antibacterial spray, characterized in that: The raw materials of the antibacterial spray include rhodiola rosea extract, saffron extract, radix ophiopogonis extract, bone-smashing extract, angelica extract, clematis extract, ligusticum chuanxiong extract, radix oleifera extract, herba striatae extract, herba striatae extract, salvia miltiorrhizae extract, cassia twig extract, turpentine, borneol, trichlorohydroxydiphenyl ether, lavender extract and auxiliary liquid.

2. The antibacterial spray according to claim 1, characterized in that: The raw materials of the antibacterial spray are respectively as follows by weight percentage: Rhodiola rosea extract 0.01%-0.5%, saffron extract 0.01%-0.4%, Lamiophloea chinensis extract 0.01%-0.8%, Bone-smacking extract 0.01%-0.3%, Angelica sinensis extract 0.01%-0.3%, Clematis chinensis extract 0.01%-0.3%, Ligusticum chuanxiong extract 0.01%-0.3%, Herba schizonepetae radix 0.01%-0.3%, Herba Lycopodii herba schizonepetae extract 0.01%-0.3%, Salvia miltiorrhizae extract 0.01%-0.3%, Cinnamomum cassiae extract 0.01%-0.3%, turpentine 0.1%-0.3%, borneol 0.3%-0.6%, trichlorohydroxydiphenyl ether 0.1%-0.25%, lavender extract 0.3%-0.5%, and the rest are auxiliary liquids.

3. The antibacterial spray according to claim 2, characterized in that: The auxiliary liquid comprises ethanol and purified water, and by weight percentage: ethanol is 25%-40%, and the rest is purified water.

4. A method for preparing the antibacterial spray according to any one of claims 1 to 3, characterized in that: include: Mixing Rhodiola rosea extract, saffron extract, Lamiophloea lappa extract, Boneless Plant extract, Angelica sinensis extract, Clematis chinensis extract, Ligusticum chuanxiong extract, Herba schizonepetae radix, Herba Lycopodii extract, Salvia miltiorrhizae extract, and Cinnamomum cassiae extract to obtain a mixed solution A; Mix turpentine, borneol, trichlorohydroxydiphenyl ether and lavender extract, and stir to obtain a mixed solution B; The obtained mixed solution A and auxiliary liquid are mixed and stirred, and then the mixed solution B is added and stirred. After standing, an antibacterial spray product is obtained.

5. The preparation method according to claim 4, characterized in that: The turpentine oil, the borneol, the trichlorohydroxy diphenyl ether and the lavender extract are mixed according to weight percentage, and stirred to obtain the mixed solution B, which includes: The turpentine oil, the borneol, the trichlorohydroxydiphenyl ether and the lavender extract are mixed, and stirred at 20-26° C. to obtain the mixed solution B.

6. The preparation method according to claim 5, characterized in that: The stirring time is between 30-45 min.

7. The preparation method according to claim 4, characterized in that: The obtained mixed solution A and the auxiliary liquid are mixed and stirred, and then the mixed solution B is added and stirred. After standing, the antibacterial spray product is obtained, including: The obtained mixed solution A and the auxiliary liquid are mixed, stirred at 20-26° C., and then the mixed solution B is slowly added and stirred, and after standing, the antibacterial spray product is obtained; and / or, The auxiliary liquid is obtained by mixing purified water and ethanol.

8. The preparation method according to claim 6, characterized in that: The duration of the two stirrings is between 30-60 minutes; and / or the standing time is between 1-36 hours.

9. The use of the antibacterial spray according to any one of claims 1 to 3, characterized in that: The antibacterial spray is used for inhibiting Staphylococcus aureus, Escherichia coli or Candida albicans.

10. The use of the antibacterial spray according to any one of claims 1 to 3, characterized in that: The antibacterial spray is used for skin and mucous membrane hygiene.

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