Cellulose degrading bacterium separation device and method

By designing a cellulose degradation bacteria separation device containing a pretreatment chamber and a separation chamber, initial separation is performed using a stirring rod and an eccentric wheel-driven screen, and further separation is achieved through a centrifuge, the existing device's simple structure and lack of pretreatment capabilities are solved, the separation purity and efficiency are improved, and the equipment life is extended.

CN119842464APending Publication Date: 2025-04-18JIANGSU UNIV OF TECH
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Patent Information

Application Number
CN202510024315.8
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-07
Publication Date
2025-04-18

AI Technical Summary

Technical Problem

The existing cellulose degradation bacteria separation device is simple in structure and lacks pretreatment capabilities, which leads to the centrifuge being easily damaged, the microbial separation purity is low, and the efficiency and accuracy are poor.

Method used

A separation device including a pretreatment chamber and a separation chamber is designed. The pretreatment chamber is equipped with a stirring rod and an eccentric wheel-driven screen for initial separation of large particles of impurities; the separation of the indoor chamber is equipped with a centrifuge, and further separation of microorganisms and soil is achieved through high-speed rotation. In addition, the device is equipped with a vacuum cleaner system and a temperature control system for keeping the separation device clean and providing suitable temperature conditions.

Benefits of technology

Effectively intercept and treat large particles of impurities in the soil, improve the separation purity of microorganisms, extend the service life of the centrifuge, improve the efficiency and accuracy of the separation process, and ensure the activity of microorganisms.

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Abstract

The invention relates to the technical field of microbial engineering, and discloses a cellulose degrading bacterium separation device and method, the cellulose degrading bacterium separation device comprises a base, a separation chamber is arranged in the base, a pretreatment chamber is assembled at the top of the base, and a first feeding port is assembled at the top of the pretreatment chamber; a second feeding port is formed between the separation chamber and the pretreatment chamber, a pretreatment assembly is arranged in the pretreatment chamber, a second discharging port is formed in the middle of the separation chamber, an opening and closing assembly and a fixing assembly are arranged under the second discharging port, and dust suction assemblies are arranged on the outer side of the base and the outer side of the pretreatment chamber. Through cooperation of the pretreatment chamber, the stirring rod, the eccentric wheel, the screen and other structures, primary separation of impurities and target microorganisms is achieved, the purity of the separated microorganisms can be improved, the difficulty of follow-up screening and identification of cellulose degrading bacteria is lowered, and the efficiency and accuracy of the whole separation process are improved.
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Citation Information

Patent Citations

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  • Non-pressurised pre-treatment, enzymatic hydrolysis and fermentation of waste fractions

    WO2007036795A1