Nondestructive detection equipment and detection method for content of microorganisms in food

Abstract

The invention relates to the technical field of detection equipment, in particular to nondestructive detection equipment and a detection method for the content of microorganisms in food. In order to solve the problems that microorganism samples are contingency when being taken out, the reproduction condition of microorganisms can be influenced, and the reliability and accuracy of data are uncertain when repeated detection is carried out at different times, the following technical scheme is provided: the device comprises a bottom plate serving as a bottom support; the sample bottle is provided with a sealing cover, the sample bottle is used for containing a culture solution, a sample box is arranged on one side of the sample bottle, a cover glass is mounted on the sample box, and a glass slide mounted on the sample box is arranged below the cover glass; and the moving mechanism is mounted at the top of the bottom plate and is used for fixing a sample bottle and driving the sample bottle to move. Detection can be carried out under the condition that sample microorganisms and the content of the culture solution are not affected, and the reliability and accuracy of the detection result are guaranteed.

Description

Technical Field

[0001] The present invention relates to the technical field of detection equipment, and in particular to a non-destructive detection device and a detection method for microbial content in food. Background Art

[0002] At present, when testing the content of microorganisms in food, samples are first collected from random or designated locations in the food. After the samples are processed, appropriate culture media are selected according to the type of microorganism to be tested for cultivation, and then a portion of the cultured samples is taken out for testing.

[0003] After taking out some samples for testing, the microbial samples in the culture medium and the culture solution decrease synchronously, but the reduction may not be proportional and is accidental. This may affect the reproduction of microorganisms. When repeated testing is performed at different times, the reliability and accuracy of the data are uncertain. In view of this, the present invention proposes a non-destructive testing device and method for the content of microorganisms in food. Summary of the invention

[0004] The purpose of the present invention is to propose a non-destructive detection device and method for the microbial content in food in order to address the problem that the removal of microbial samples from culture medium is accidental, which may affect the reproduction of microorganisms, and when repeated detection is performed at different times, there is uncertainty in the reliability and accuracy of the data.

[0005] On the one hand, the present invention provides a non-destructive detection device for the content of microorganisms in food, comprising a bottom plate as a bottom support; a sample bottle with a sealing cover, the sample bottle is used to contain a culture solution, a sample box is arranged on one side of the sample bottle, a cover glass is installed on the sample box, and a slide glass installed on the sample box is arranged below the cover glass; a moving mechanism installed on the top of the bottom plate, the moving mechanism is used to fix the sample bottle and drive the sample bottle to move; a base is arranged on one side of the sample bottle, an observation eyepiece is arranged above the base, the observation eyepiece is installed on the top of the bottom plate through a rack, and the observation eyepiece is used to detect microorganisms; a pressing plate is arranged between the base and the observation eyepiece, four groups of pressing blocks are installed on the bottom of the pressing plate, and the positions of the four groups of pressing blocks correspond to the four corners of the cover glass; a liquid pressing mechanism installed on the pressing plate, the liquid pressing mechanism is used to drive the pressing plate to move and reduce the gap between the cover glass and the slide glass; a multi-stage driving mechanism installed on the top of the bottom plate, the multi-stage driving mechanism is used to drive the liquid pressing mechanism.

[0006] Optionally, the liquid pressing mechanism includes two sets of mounting plates fixedly connected to the top of the pressing plate. The two sets of mounting plates are symmetrically arranged, and the mounting plates are of an L-shaped structure. A plurality of groups of rollers are arranged at equal intervals on the mounting plates. Two sets of pressing plates are arranged on one side of the pressing plate. The pressing plates correspond to the positions of the rollers. The bottom end of the pressing plate close to the pressing plate is inclined, and a chute is formed at the bottom of the pressing plate. The width of the chute is greater than the width of the roller. A plurality of groups of connecting plates are also fixedly connected between the two sets of pressing plates. Limit strips are installed on the sides of the two sets of pressing plates away from each other. Limit blocks are slidably connected to the limit strips, and the limit blocks are installed on the frame.

[0007] Optionally, a first rotating block is rotatably connected to the opposite sides of the two sets of mounting plates. A first fixing rod is fixedly connected to the top of the first rotating block. A fixing plate is slidably connected to the first fixing rod. A first limiting disc is also installed on the top of the first fixing rod. A first spring is arranged between the fixing plate and the first limiting disc. The first spring is sleeved and installed on the outer circle of the first fixing rod.

[0008] Optionally, fixing blocks are fixedly connected to the opposite sides of the two sets of fixing plates away from each other. The fixing blocks are installed on the top of the bottom plate through the frame. Two sets of rotating plates are rotatably connected to the side of the fixing block close to the fixing plate. The two sets of rotating plates are symmetrically arranged on both sides of the fixing plate. A second fixing rod is slidably connected in the rotating plate. The bottom of the second fixing rod is fixedly connected to a second rotating block. The second rotating block is rotatably connected to the mounting plate. A sliding disc is slidably connected to the second fixing rod. The sliding disc is arranged above the rotating plate. The top of the second fixing rod is fixedly connected to a second limiting disc. A second spring is arranged between the sliding disc and the second limiting disc. The second spring is sleeved and installed on the outer circle of the second fixing rod.

[0009] Optionally, the multi-stage driving mechanism includes a mounting frame installed on the top of the bottom plate. A second push rod motor is installed on the mounting frame. The output end of the second push rod motor is fixedly connected to a synchronous plate. The synchronous plate is of a Z-shaped setting. A third push rod motor is installed on the synchronous plate. The output end of the third push rod motor is fixedly connected to a connecting plate away from the base.

[0010] Optionally, positioning plates are fixedly connected to both sides of the mounting frame. A sliding rod is fixedly connected between the positioning plate and the base. A sliding sleeve is slidably connected to the sliding rod. The two sets of sliding sleeves are respectively fixedly connected to both sides of the synchronous plate.

[0011] Optionally, the base is of a "U" shape. Two sets of support plates are installed on the top of the base. A supplementary light is also installed on the base. The supplementary light is located directly below the observation eyepiece.

[0012] Optionally, the moving mechanism includes a side plate installed on the top of the base plate, a linear motor is installed on the side of the side plate close to the sample bottle, the moving end of the linear motor is fixedly connected to the moving plate, the side of the moving plate away from the side plate is fixedly connected to a placement seat, the sample bottle is installed on the placement seat, the side of the moving plate away from the sample bottle is fixedly connected to at least one group of sliders, a slide rail is slidably connected to the slider, and the slide rail is fixedly connected to the side of the side plate.

[0013] Optionally, a first push rod motor is installed on the top of the movable plate, and the output end of the first push rod motor is fixedly connected to a push plate. A positioning belt is installed on the side of the push plate close to the sample bottle, and the positioning belt is U-shaped and is sleeved on the outside of the sample bottle.

[0014] In another aspect, the present invention provides a method for detecting microbial content in food by a non-destructive detection device, comprising the following steps: Step 1: first open the lid of the sample bottle, put culture solution into the sample bottle, the level of the culture solution is higher than the position of the sample box, take some microorganisms and put them into the culture solution in the sample bottle, and close the lid; Step 2: Pass the sample bottle through the sample box and place it on the placement seat, start the first push rod motor, and the first push rod motor drives the push plate to move, so that the positioning belt positions the sample bottle; Step 3, after the microorganisms in the sample bottle have been propagated for one hour, the linear motor is started to drive the placement seat to move, so that the sample bottle moves synchronously until the cover glass and the slide glass move to the top of the base and are located directly below the pressing plate; Step 4, start the second push rod motor and the third push rod motor, the two groups of extrusion plates move smoothly under the limiting action of the limiting strip and the limiting block, after the inclined position of the extrusion plate contacts the roller, the mounting plate is driven to tilt, the pressing plate is synchronously deflected, and at the same time, the two groups of pressing blocks close to the multi-stage driving mechanism first contact the cover glass, and when moving downward, one end of the cover glass is squeezed downward to approach the slide first, when the extrusion plate is completely moved to the top of the multiple groups of rollers, the cover glass is attached to the top of the slide, and the sample in the sample box is squeezed out to eliminate the gas generated when the microorganisms reproduce, and at the same time, the sample between the cover glass and the slide is a thin layer; Step 5: Turn on the fill light to perform fill light and detection at the same time to improve the detection accuracy; Step 6: After a test, the second push rod motor and the third push rod motor are synchronously contracted, and the first spring and the second spring are synchronously released, so that the mounting plate moves upward and drives the pressing block away from the cover glass, so that the sample box releases the elastic force, the cover glass moves away from the slide, and the sample in the sample bottle enters the sample box again, and the microorganisms continue to multiply; Step seven: the microorganisms in the sample reproduce for another hour and then are tested again. This cycle is repeated multiple times and the samples are not affected, thus ensuring the accuracy of the test results.

[0015] In summary, the present application includes at least one of the following beneficial technical effects: The present invention sets a multi-stage driving mechanism. After starting, the second push rod motor and the third push rod motor drive the extrusion plate to move, and cooperate with the roller to realize the tilting and downward pressing of the pressing plate, so that the cover glass is tilted and close to the slide glass, the gap between the cover glass and the slide glass is reduced, and bubbles are eliminated, which is convenient for testing the sample through the observation eyepiece and improves the accuracy of the detection. Furthermore, by setting the sample bottle, the culture solution in the sample bottle is always in a closed environment, so that the ratio of microorganisms to culture solution does not change after the test, which is convenient for intermittent testing of microbial reproduction and ensures the reliability and accuracy of the test results; In summary, the present invention can perform detection without affecting the content of sample microorganisms and culture fluid, thereby ensuring the reliability and accuracy of the detection results. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a schematic diagram of the structure of a non-destructive testing device for microbial content in food; Figure 2 is a schematic diagram of the cross-sectional structure of the sample bottle; Figure 3 It is a schematic diagram of the cross-sectional structure of the base; Figure 4 It is a structural schematic diagram of the hydraulic mechanism; Figure 5 It is a schematic diagram of the structure of the extruded plate; Figure 6 yes Figure 5 The enlarged schematic diagram of point A in the middle; Figure 7 yes Figure 5 A magnified schematic diagram of point B in the middle.

[0017] Reference numerals: 1. Bottom plate; 2. Sample bottle; 21. Sample box; 22. Cover glass; 23. Slide; 3. Moving mechanism; 31. Side plate; 32. Linear motor; 33. Moving plate; 34. Placement seat; 35. Sliding block; 36. Slide rail; 37. First push rod motor; 38. Push plate; 39. Positioning belt; 4. Base; 41. Support plate; 42. Fill light; 5. Observation eyepiece; 6. Pressing plate; 61. Pressing block; 7. Liquid-pressing mechanism; 701. Mounting plate; 702. Roller; 703. Extrusion plate; 704. Slide; 705. Connecting plate; 706. First rotating block; 707. First fixing rod; 708. Fixing plate; 709. First limiting plate; 710. First spring; 711. Fixing block; 712. Rotating plate; 713. Second fixing rod; 714. Second rotating block; 715. Sliding plate; 716. Second limiting plate; 717. Second spring; 718. Limiting strip; 719. Limiting block; 8. Multi-stage driving mechanism; 81. Mounting frame; 82. Second push rod motor; 83. Synchronous plate; 84. Third push rod motor; 85. Positioning plate; 86. Sliding rod; 87. Sliding sleeve. DETAILED DESCRIPTION

[0018] The technical solution of the present invention will be clearly and completely described below in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments.

[0019] The components of the embodiments of the present invention generally described and shown in the drawings herein may be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention.

[0020] Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative work shall fall within the scope of protection of the present invention.

[0021] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0022] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, or it can be the internal communication of two components. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances. Example

[0023] As Figure 1 and Figure 2 shown, a non-destructive detection device for the microbial content in food proposed by the present invention includes a bottom plate 1 as the bottom support, a sample bottle 2 with a sealed cover. The sample bottle 2 is used to hold a culture solution, and microorganisms reproduce in the culture solution to form a sample. A sample box 21 is arranged on one side of the sample bottle 2. The sample box 21 is made of rubber and has elasticity, so that the cover glass 22 can be close to the slide 23. The sample fills the inside of the sample box 21. A cover glass 22 is installed on the sample box 21, and a slide 23 installed on the sample box 21 is arranged below the cover glass 22. When the cover glass 22 is close to the slide 23, it is convenient to detect the sample.

[0024] Further, the above detection device includes a moving mechanism 3 installed on the top of the bottom plate 1. The moving mechanism 3 is used to fix the sample bottle 2 and drive the sample bottle 2 to move. The moving mechanism 3 includes side plates 31 installed on the top of the bottom plate 1, and the positions of the side plates 31 are fixed. A linear motor 32 is installed on one side of the side plate 31 close to the sample bottle 2. The moving end of the linear motor 32 is fixedly connected with a moving plate 33. The linear motor 32 is used to drive the moving plate 33 to move. A placing seat 34 is fixedly connected to the side of the moving plate 33 away from the side plate 31. The sample bottle 2 is installed on the placing seat 34. The placing seat 34 moves synchronously with the moving plate 33 and drives the sample bottle 2 to move at the same time. Two groups of sliders 35 are fixedly connected to the side of the moving plate 33 away from the sample bottle 2. A slide rail 36 is slidably connected in the sliders 35. The slide rail 36 is fixedly connected to the side of the side plate 31. The moving plate 33 moves smoothly under the limiting action of the sliders 35 and the slide rail 36. A first push rod motor 37 is installed on the top of the moving plate 33. The output end of the first push rod motor 37 is fixedly connected with a push plate 38. A positioning belt 39 is installed on the side of the push plate 38 close to the sample bottle 2. The positioning belt 39 is arranged in a U shape and is sleeved on the outside of the sample bottle 2. The first push rod motor 37 is used to drive the push plate 38 to move and drive the positioning belt 39 to squeeze the sample bottle 2 to fix the sample bottle 2.

[0025] Even further, please refer to Figure 1 and Figure 3 , the above detection device further includes a base 4 arranged on one side of the sample bottle 2. The base 4 is arranged in a "U" shape. Two groups of support plates 41 are installed on the top of the base 4. The support plates 41 are located below the slide 23 and are used to support the slide 23. A supplementary light lamp 42 is also installed on the base 4. The supplementary light lamp 42 is located directly below the observation eyepiece 5 and is used for supplementary lighting to facilitate the observation eyepiece 5 to detect the reproduction status of microorganisms. An observation eyepiece 5 is arranged above the base 4. The observation eyepiece 5 is installed on the top of the bottom plate 1 through a frame and is used to detect microorganisms.

[0026] It is worth mentioning that, as Figure 4 and Figure 5 As shown, the above-mentioned detection equipment includes a pressing plate 6 arranged between the base 4 and the observation eyepiece 5, and four groups of pressing blocks 61 are installed at the bottom of the pressing plate 6. The positions of the four groups of pressing blocks 61 correspond to the four corners of the cover glass 22. When the pressing plate 6 moves downward, it can contact the cover glass 22 through the pressing blocks 61 and squeeze the cover glass 22 to make it close to the slide glass 23, so as to facilitate detection.

[0027] For further information, see Figures 4 to 7The above-mentioned detection device includes a liquid pressure mechanism 7 installed on the pressing plate 6, and the liquid pressure mechanism 7 is used to drive the pressing plate 6 to move and reduce the gap between the cover glass 22 and the slide glass 23. The liquid pressure mechanism 7 includes two groups of mounting plates 701 fixedly connected to the top of the pressing plate 6, and the two groups of mounting plates 701 are symmetrically arranged, and the mounting plates 701 move synchronously with the pressing plate 6. The mounting plate 701 is an L-shaped structure, and the mounting plate 701 is provided with five groups of rollers 702 in an equidistant array, and the rollers 702 move synchronously with the mounting plate 701. Two groups of squeezing plates 703 are arranged on one side of the pressing plate 6, and the squeezing plates 703 correspond to the positions of the rollers 702. The bottom of the squeezing plate 703 close to the pressing plate 6 is inclined, so that when the squeezing plate 703 contacts the roller 702, the roller 702 is driven to move downward through the inclined setting of the bottom of the squeezing plate 703. A slide groove 704 is provided at the bottom of the extrusion plate 703, and the width of the slide groove 704 is greater than the width of the roller 702, so that the installation plate 701 can be driven downward by the roller 702 when the extrusion plate 703 moves. Two groups of connecting plates 705 are also fixedly connected between the two groups of extrusion plates 703, so that the structure of the extrusion plate 703 is stable. Limiting strips 718 are installed on the sides of the two groups of extrusion plates 703 that are away from each other, and the limiting strips 718 move synchronously with the extrusion plates 703. The limiting strips 718 are slidably connected to the limiting blocks 719, which are installed on the frame. The position of the limiting blocks 719 is fixed, and they cooperate with the limiting strips 718 to limit the movement of the extrusion plates 703, so that the movement of the extrusion plates 703 is stable. The first rotating block 706 is rotatably connected to the opposite side of the two sets of mounting plates 701. The top of the first rotating block 706 is fixedly connected to the first fixing rod 707. The first fixing rod 707 is rotatably connected to the mounting plate 701 through the first rotating block 706. A fixing plate 708 is slidably connected to the first fixing rod 707, and the position of the fixing plate 708 is fixed. A first limiting plate 709 is also installed on the top of the first fixing rod 707. A first spring 710 is arranged between the fixing plate 708 and the first limiting plate 709. The first spring 710 is sleeved and installed on the outer ring of the first fixing rod 707, and is used to release the elastic force so that the mounting plate 701 is fixed at a high place. At the same time, the first spring 710 is compressed when the mounting plate 701 moves downward. The two sets of fixing plates 708 are fixedly connected to the sides away from each other with a fixing block 711. The fixing block 711 is installed on the top of the bottom plate 1 through a frame, and the position of the fixing block 711 is fixed. The fixed block 711 is also rotatably connected to one side of the fixed plate 708 with two groups of rotating plates 712, and the two groups of rotating plates 712 are symmetrically arranged on both sides of the fixed plate 708. A second fixed rod 713 is slidably connected in the rotating plate 712, and a second rotating block 714 is fixedly connected to the bottom of the second fixed rod 713. The second rotating block 714 is rotatably connected to the mounting plate 701, and the second fixed rod 713 is rotatably connected to the mounting plate 701 through the second rotating block 714.The second fixed rod 713 is slidably connected with a sliding plate 715, which is arranged above the rotating plate 712. The top of the second fixed rod 713 is fixedly connected with a second limiting plate 716. A second spring 717 is arranged between the sliding plate 715 and the second limiting plate 716. The second spring 717 is sleeved and installed on the outer ring of the second fixed rod 713. The second limiting plate 716 releases the elastic force to make the sliding plate 715 close to the rotating plate 712. After the extrusion plate 703 contacts the roller 702, the mounting plate 701 is driven to tilt. At the same time, the second fixed rod 713 slides in the rotating plate 712, and the rotating plate 712 is deflected to make it parallel to the mounting plate 701. The elastic force of the second spring 717 ensures that the mounting plate 701 moves smoothly. Thus, the mounting plate 701 is first tilted, then turned into a horizontal state, and moves downward while deflecting, so that the two groups of pressing blocks 61 close to the multi-stage driving mechanism 8 first press down the cover glass 22, so that one end of the cover glass 22 first approaches the slide glass 23, and then slowly covers the slide glass 23, so as to facilitate the removal of bubbles generated by the reproduction of microorganisms.

[0028] Finally, the above-mentioned detection device also includes a multi-stage drive mechanism 8 installed on the top of the base plate 1, and the multi-stage drive mechanism 8 is used to drive the hydraulic mechanism 7. The multi-stage drive mechanism 8 includes a mounting frame 81 installed on the top of the base plate 1, and a second push rod motor 82 is installed on the mounting frame 81. The output end of the second push rod motor 82 is fixedly connected to a synchronization plate 83, and the second push rod motor 82 is used to drive the synchronization plate 83 to move. The synchronization plate 83 is arranged in a Z shape, and a third push rod motor 84 is installed on the synchronization plate 83. After the second push rod motor 82 is started, the third push rod motor 84 is driven to move through the synchronization plate 83. The output end of the third push rod motor 84 is fixedly connected to a group of connecting plates 705 away from the base 4. After the third push rod motor 84 is started, it drives the connecting plate 705 to move, thereby driving the two groups of extrusion plates 703 to move. Positioning plates 85 are fixedly connected to both sides of the mounting frame 81, a sliding rod 86 is fixedly connected between the positioning plate 85 and the base 4, a sliding sleeve 87 is slidably connected to the sliding rod 86, and two sets of sliding sleeves 87 are respectively fixedly connected to both sides of the synchronization plate 83 for limiting the synchronization plate 83 so that the movement of the synchronization plate 83 is smooth.

[0029] In this embodiment, the lid of the sample bottle 2 is first opened, and the culture solution is placed in the sample bottle 2, the culture solution level is higher than the position of the sample box 21, and some microorganisms are taken and placed in the culture solution in the sample bottle 2, and the lid is closed. The sample bottle 2 passes through the sample box 21 and is placed on the placement seat 34, and the first push rod motor 37 is started, and the first push rod motor 37 drives the push plate 38 to move, so that the positioning belt 39 positions the sample bottle 2. After the microorganisms in the sample bottle 2 have been propagated for one hour, the linear motor 32 is started, and the linear motor 32 drives the moving plate 33 to move, and the moving plate 33 moves smoothly under the limiting action of the slider 35 and the slide rail 36. At the same time, the placement seat 34 is driven to move, so that the sample bottle 2 moves synchronously until the cover glass 22 and the slide glass 23 move to the top of the base 4 and are located directly below the pressing plate 6. At this time, the second push rod motor 82 and the third push rod motor 84 are started, and the second push rod motor 82 is extended to drive the synchronous plate 83 to move under the limiting action of the slide bar 86 and the sliding sleeve 87, and drives the third push rod motor 84 to move. Thereby, the connecting plate 705 is driven to move, and the two groups of squeezing plates 703 move smoothly under the limiting action of the limiting strip 718 and the limiting block 719. After the inclined position of the squeezing plate 703 contacts the roller 702, it drives the mounting plate 701 to tilt, and at the same time, the four groups of second fixing rods 713 all slide in the rotating plate 712, and at the same time, the rotating plate 712 deflects to maintain parallelism with the mounting plate 701. As the squeezing plate 703 continues to move, the second spring 717 and the first spring 710 are continuously compressed. At the same time, when the mounting plate 701 deflects, the pressing plate 6 deflects synchronously, and at the same time, the two groups of pressing blocks 61 close to the multi-stage driving mechanism 8 first contact the cover glass 22, and when moving downward, one end of the squeezing cover glass 22 moves downward and first approaches the slide glass 23. When the squeezing plate 703 is completely moved above the multiple groups of rollers 702, the cover glass 22 is attached to the slide glass 23, and the sample in the sample box 21 is squeezed out. Since the cover glass 22 is slowly transformed from an inclined state to a horizontal state and covers the slide glass 23, it is convenient to remove the gas generated when the microorganisms reproduce. At the same time, the sample between the cover glass 22 and the slide glass 23 is a thin layer, which is convenient for detection. During the detection, the fill light 42 is turned on to fill light to improve the detection accuracy. After one detection, the second push rod motor 82 and the third push rod motor 84 are synchronously contracted, and the first spring 710 and the second spring 717 are synchronously released, so that the mounting plate 701 moves upward and drives the pressing block 61 away from the cover glass 22. As a result, the sample box 21 releases the elastic force, the cover glass 22 is away from the slide glass 23, and the sample in the sample bottle 2 enters the sample box 21 again. The microorganisms in the sample reproduce for another hour and are tested again. After multiple tests, the sample is guaranteed not to be affected and the accuracy of the test results is guaranteed.

[0030] A method for detecting microbial content in food by non-destructive testing equipment, comprising the following steps: Step 1: first open the lid of the sample bottle 2, put the culture solution in the sample bottle 2, the level of the culture solution is higher than the position of the sample box 21, and take some microorganisms and put them into the culture solution in the sample bottle 2, and close the lid; Step 2: Pass the sample bottle 2 through the sample box 21 and place it on the placement seat 34, start the first push rod motor 37, and the first push rod motor 37 drives the push plate 38 to move, so that the positioning belt 39 positions the sample bottle 2; Step 3: After the microorganisms in the sample bottle 2 have been grown for one hour, the linear motor 32 is started to drive the placement seat 34 to move, so that the sample bottle 2 moves synchronously until the cover glass 22 and the slide glass 23 move to the top of the base 4 and are located directly below the pressing plate 6; Step 4, start the second push rod motor 82 and the third push rod motor 84, and the two groups of squeezing plates 703 move smoothly under the limiting action of the limiting strip 718 and the limiting block 719. After the inclined position of the squeezing plate 703 contacts the roller 702, it drives the mounting plate 701 to tilt, and the pressing plate 6 deflects synchronously. At the same time, the two groups of pressing blocks 61 close to the multi-stage driving mechanism 8 first contact the cover glass 22, and squeeze one end of the cover glass 22 to move downward and approach the slide glass 23 when moving downward. When the squeezing plate 703 is completely moved to the top of the multiple groups of rollers 702, the cover glass 22 is attached to the top of the slide glass 23, and the sample in the sample box 21 is squeezed out to eliminate the gas generated when the microorganisms reproduce. At the same time, the sample between the cover glass 22 and the slide glass 23 is a thin layer; Step 5: The fill light 42 is turned on to perform fill light and detection at the same time, so as to improve the detection accuracy; Step 6: After one test, the second push rod motor 82 and the third push rod motor 84 are synchronously contracted, and the first spring 710 and the second spring 717 are synchronously released, so that the mounting plate 701 moves upward and drives the pressing block 61 away from the cover glass 22, so that the sample box 21 releases the elastic force, the cover glass 22 moves away from the slide glass 23, and the sample in the sample bottle 2 enters the sample box 21 again, and the microorganisms continue to multiply; Step seven: the microorganisms in the sample reproduce for another hour and then are tested again. This cycle is repeated multiple times and the samples are not affected, thus ensuring the accuracy of the test results.

[0031] The above specific embodiment is only an optional embodiment of the present invention. Based on the technical solution of the present invention and the relevant inspiration of the above embodiment, those skilled in the art can make various alternative improvements and combinations to the above specific embodiment.

Claims

1. A non-destructive testing device for microbial content in food, characterized in that: include: A base plate (1) as a bottom support; A sample bottle (2) with a sealing cover, the sample bottle (2) being used to contain a culture solution, a sample box (21) being arranged on one side of the sample bottle (2), a cover glass (22) being mounted on the sample box (21), and a glass slide (23) mounted on the sample box (21) being arranged below the cover glass (22); A moving mechanism (3) installed on the top of the base plate (1), the moving mechanism (3) being used to fix the sample bottle (2) and drive the sample bottle (2) to move; A base (4) is arranged on one side of the sample bottle (2), an observation eyepiece (5) is arranged above the base (4), and the observation eyepiece (5) is installed on the top of the bottom plate (1) through a frame, and the observation eyepiece (5) is used to detect microorganisms; A pressure plate (6) disposed between the base (4) and the observation eyepiece (5), wherein four groups of pressure blocks (61) are installed at the bottom of the pressure plate (6), and the positions of the four groups of pressure blocks (61) correspond to the four corners of the cover glass (22); A liquid pressing mechanism (7) mounted on the pressing plate (6), the liquid pressing mechanism (7) being used to drive the pressing plate (6) to move and reduce the gap between the cover glass (22) and the slide glass (23); A multi-stage driving mechanism (8) is installed on the top of the base plate (1), and the multi-stage driving mechanism (8) is used to drive the liquid pressing mechanism (7).

2. The non-destructive testing device for microbial content in food according to claim 1, characterized in that: The liquid pressing mechanism (7) comprises two groups of mounting plates (701) fixedly connected to the top of the pressing plate (6), the two groups of mounting plates (701) being symmetrically arranged, and the mounting plates (701) being an L-shaped structure, the mounting plates (701) being arranged in an equidistant array with a plurality of groups of rollers (702), and two groups of extrusion plates (703) being arranged on one side of the pressing plate (6), the positions of the extrusion plates (703) corresponding to the rollers (702), and the extrusion plates (703) being close to the pressing plate (6). The bottom of one end is inclined, and a slide groove (704) is provided at the bottom of the extrusion plate (703), the width of the slide groove (704) is greater than the width of the roller (702), and a plurality of connection plates (705) are fixedly connected between the two groups of extrusion plates (703), and a limit strip (718) is installed on the side of the two groups of extrusion plates (703) away from each other, and a limit block (719) is slidably connected to the limit strip (718), and the limit block (719) is installed on the frame.

3. A non-destructive testing device for microbial content in food according to claim 2, characterized in that: The two groups of mounting plates (701) are rotatably connected to a first rotating block (706) on one side opposite to the other. The top of the first rotating block (706) is fixedly connected to a first fixing rod (707). A fixing plate (708) is slidably connected to the first fixing rod (707). A first limiting plate (709) is also installed on the top of the first fixing rod (707). A first spring (710) is provided between the fixing plate (708) and the first limiting plate (709). The first spring (710) is sleeved and installed on the outer ring of the first fixing rod (707).

4. The non-destructive testing device for microbial content in food according to claim 3, characterized in that: On one side of the two sets of the fixed plates (708) away from each other, there are fixed blocks (711) fixedly connected. The fixed blocks (711) are installed on the top of the bottom plate (1) through a frame. On one side of the fixed block (711) close to the fixed plate (708), there are also two sets of rotating plates (712) rotatably connected. The two sets of rotating plates (712) are symmetrically arranged on both sides of the fixed plate (708). A second fixed rod (713) is slidably connected in the rotating plate (712). The bottom of the second fixed rod (713) is fixedly connected with a second rotating block (714). The second rotating block (714) is rotatably connected to the mounting plate (701). A sliding disk (715) is slidably connected to the second fixed rod (713). The sliding disk (715) is arranged above the rotating plate (712). The top of the second fixed rod (713) is fixedly connected with a second limiting disk (716). A second spring (717) is arranged between the sliding disk (715) and the second limiting disk (716). The second spring (717) is sleeved and installed on the outer circle of the second fixed rod (713).

5. The non-destructive testing device for microbial content in food according to claim 2, characterized in that: The multi-stage driving mechanism (8) includes a mounting frame (81) installed on the top of the bottom plate (1). A second push rod motor (82) is installed on the mounting frame (81). The output end of the second push rod motor (82) is fixedly connected with a synchronous plate (83). The synchronous plate (83) is Z-shaped. A third push rod motor (84) is installed on the synchronous plate (83). The output end of the third push rod motor (84) is fixedly connected with a connecting plate (705) away from the base (4).

6. The non-destructive testing device for microbial content in food according to claim 5, characterized in that: On both sides of the mounting frame (81), there are positioning plates (85) fixedly connected. A sliding rod (86) is fixedly connected between the positioning plate (85) and the base (4). A sliding sleeve (87) is slidably connected to the sliding rod (86). The two sets of sliding sleeves (87) are respectively fixedly connected to both sides of the synchronous plate (83).

7. The non-destructive testing device for microbial content in food according to claim 1, characterized in that: The base (4) is in a "U" shape. Two sets of support plates (41) are installed on the top of the base (4). A supplementary light (42) is also installed on the base (4). The supplementary light (42) is located directly below the observation eyepiece (5).

8. The non-destructive testing device for microbial content in food according to claim 1, characterized in that: The moving mechanism (3) includes a side plate (31) installed on the top of the bottom plate (1). A linear motor (32) is installed on one side of the side plate (31) close to the sample bottle (2). The moving end of the linear motor (32) is fixedly connected with a moving plate (33). On one side of the moving plate (33) away from the side plate (31), there is a placing seat (34) fixedly connected. The sample bottle (2) is installed on the placing seat (34). On one side of the moving plate (33) away from the sample bottle (2), there is at least one set of sliders (35) fixedly connected. A slide rail (36) is slidably connected in the slider (35). The slide rail (36) is fixedly connected to the side of the side plate (31).

9. The non-destructive testing device for microbial content in food according to claim 8, characterized in that: A first push rod motor (37) is installed on the top of the movable plate (33); an output end of the first push rod motor (37) is fixedly connected to a push plate (38); a positioning belt (39) is installed on the side of the push plate (38) close to the sample bottle (2); the positioning belt (39) is U-shaped and is sleeved on the outside of the sample bottle (2).

10. A method for detecting the content of microorganisms in food according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1: first, open the lid of the sample bottle (2), put culture solution into the sample bottle (2), the liquid level of the culture solution is higher than the position of the sample box (21), and take some microorganisms and put them into the culture solution in the sample bottle (2), and then close the lid; Step 2: Pass the sample bottle (2) through the sample box (21) and place it on the placement seat (34), start the first push rod motor (37), and the first push rod motor (37) drives the push plate (38) to move, so that the positioning belt (39) positions the sample bottle (2); Step three, after the microorganisms in the sample bottle (2) have been growing for one hour, the linear motor (32) is started to drive the placement seat (34) to move, so that the sample bottle (2) moves synchronously until the cover glass (22) and the slide glass (23) move to the top of the base (4) and are located directly below the pressing plate (6); Step 4, starting the second push rod motor (82) and the third push rod motor (84), the two groups of squeezing plates (703) move smoothly under the limiting action of the limiting strip (718) and the limiting block (719), after the inclined position of the squeezing plate (703) contacts the roller (702), the mounting plate (701) is driven to tilt, the pressing plate (6) is synchronously deflected, and at the same time, the two groups of pressing blocks (61) close to the multi-stage driving mechanism (8) first contact the cover glass (22), and when moving downward, squeeze one end of the cover glass (22) to move downward and first approach the slide glass (23), when the squeezing plate (703) is completely moved above the multiple groups of rollers (702), the cover glass (22) is attached to the slide glass (23), and the sample in the sample box (21) is squeezed out to remove the gas generated when the microorganisms reproduce, and at the same time, the sample between the cover glass (22) and the slide glass (23) is a thin layer; Step 5: The fill light (42) is turned on to perform fill light while performing detection, thereby improving detection accuracy; Step 6: After a test is performed, the second push rod motor (82) and the third push rod motor (84) are contracted synchronously, and the first spring (710) and the second spring (717) are released synchronously, so that the mounting plate (701) moves upward and drives the pressing block (61) away from the cover glass (22), so that the sample box (21) releases the elastic force, the cover glass (22) moves away from the slide glass (23), and the sample in the sample bottle (2) enters the sample box (21) again, and the microorganisms continue to multiply; Step seven: the microorganisms in the sample reproduce for another hour and then are tested again. This cycle is repeated multiple times and the samples are not affected, thus ensuring the accuracy of the test results.

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