Microorganism sterile culture device

Abstract

The invention discloses a microorganism sterile culture device and relates to the technical field of microorganism culture. Comprising a tank body, and a cover body is fixedly connected to the upper side of the tank body; the floating ring is slidably connected in the tank body, the floating ring is fixedly connected with a mounting shell, the mounting shell is provided with an air guide pump, an oxygen dissolving cylinder is slidably connected in the mounting shell in a sealed mode, a tension spring is fixedly connected between the mounting shell and the oxygen dissolving cylinder, the mounting shell and the oxygen dissolving cylinder jointly form a power cavity, an air outlet pipe of the air guide pump is communicated with the power cavity, and the air outlet pipe of the air guide pump is communicated with the power cavity. And the lower part of the oxygen dissolving cylinder is provided with a liquid inlet hole for allowing a culture solution to enter the oxygen dissolving cylinder. A part of the culture solution in the tank body is transferred into the dissolved oxygen cylinder, the culture solution in the dissolved oxygen cylinder is separated from the culture solution in the tank body, and oxygen is introduced into the dissolved oxygen cylinder, so that the dissolved oxygen concentration of the culture solution in the tank body is improved while the culture solution in the tank body is not disturbed, and normal growth and feeding of the czochralski are guaranteed; and the breeding rate of the czooptera is ensured.

Description

Technical Field

[0001] The invention relates to the technical field of microbial cultivation, and in particular to a microbial aseptic cultivation device. Background Art

[0002] The bell worm is an indicator organism that can be used to monitor the effect of wastewater treatment and predict the quality of effluent. Therefore, there is a practical need for the cultivation and reproduction of the bell worm. As an aerobic microorganism, the bell worm is extremely sensitive to the dissolved oxygen concentration in the culture medium. The ideal dissolved oxygen concentration should be maintained between 3-5 mg / L. When the dissolved oxygen concentration in the culture medium is not between 3-5 mg / L, the feeding, movement and other physiological activities of the bell worm will be disordered and even die due to the inability to adapt to the culture environment.

[0003] When eating, the bell worm creates water flow by constantly swinging the cilia on its head, which carries tiny particles such as bacteria, algae, and organic debris in the surrounding water into its mouth. However, when cultivating bell worms, in order to quickly increase the dissolved oxygen concentration in the culture medium, oxygen is generally introduced into the water and disturbed to cause the oxygen to dissolve in the culture medium. However, the change in water flow makes it difficult for the bell worm to capture tiny particles for feeding, affecting its growth and reproduction. Summary of the invention

[0004] The present invention provides a microbial aseptic culture device to overcome the disadvantages of the prior art that, when culturing bell worms, the dissolved oxygen concentration in the culture solution is increased by introducing oxygen into the culture solution, which causes water flow fluctuations in the culture solution, affects the feeding of the bell worms, and further affects the growth and reproduction of the bell worms.

[0005] The technical implementation scheme of the present invention is: a microbial aseptic culture device, comprising: A tank body, wherein a cover body is fixedly connected to the upper side of the tank body; a floating ring, slidably connected in the tank body, the floating ring is fixedly connected with a mounting shell, the mounting shell is installed with an air pump, the mounting shell is sealingly and slidably connected with a dissolved oxygen cylinder, and a tension spring is fixedly connected between the two, the mounting shell and the dissolved oxygen cylinder jointly form a power chamber, the air outlet pipe of the air pump is connected with the power chamber, the lower part of the dissolved oxygen cylinder is provided with a liquid inlet hole for allowing the culture solution to enter therein, the upper part of the dissolved oxygen cylinder is provided with an exhaust hole, and the mounting shell is provided with a connecting hole connecting the power chamber with the inside of the tank body; An air delivery pipe is fixedly connected to the dissolved oxygen cylinder and communicated with the power chamber, and an air delivery one-way valve is installed in the air delivery pipe; A plugging assembly is arranged on the dissolved oxygen cylinder and is used for plugging the liquid inlet and the exhaust hole.

[0006] Furthermore, the blocking component comprises: A shielding member, which is position-limited and sealingly slidably connected to the dissolved oxygen cylinder and is used to block the liquid inlet and the exhaust hole; A limiting ring is fixedly connected to the mounting shell, and the limiting ring is used to support the shielding member.

[0007] Furthermore, it also includes: The exhaust solenoid valve is installed on the upper side of the installation shell. The dissolved oxygen cylinder is provided with a circulation hole. The exhaust solenoid valve is connected with the circulation hole through a telescopic conduit.

[0008] Furthermore, it also includes: A distribution plate is sealed and rotatably connected to the gas pipeline, the distribution plate is communicated with the gas pipeline, a side of the distribution plate close to the air pump is provided with a plurality of dissolved oxygen through holes communicated with the interior thereof, and a side of the distribution plate away from the central axis thereof is provided with a plurality of power through holes communicated with the interior thereof.

[0009] Furthermore, it also includes: The stirring member is fixedly connected to the distribution plate.

[0010] Furthermore, it also includes: A sealing ring is sealingly and slidably connected in the mounting shell, a spring is fixedly connected between the sealing ring and the mounting shell, a waste discharge channel is provided in the mounting shell and the floating ring, the waste discharge channel is communicated with the power chamber, the sealing ring is used to block the waste discharge channel, the floating ring is provided with a waste extraction groove, the waste extraction groove is communicated with the waste discharge channel; A three-way pipe is fixedly connected to the air pump and communicated with the air inlet pipe of the air pump. An oxygen extraction one-way valve is installed in one outlet of the three-way pipe. The cover body is installed with a carbon exhaust one-way valve communicated with the outside world. The carbon exhaust one-way valve is communicated with the other outlet of the three-way pipe through a telescopic guide tube.

[0011] Furthermore, it also includes: A connecting disk is sealed and rotatably connected to the lower side of the dissolved oxygen cylinder, the connecting disk is fixedly connected to and communicated with a plurality of oxygen distribution membrane wires, and the oxygen distribution membrane wires are communicated with the interior of the dissolved oxygen cylinder through the connecting disk; The rotating assembly is arranged in the connecting plate and is used to control the flow direction of the culture solution between the dissolved oxygen cylinder and the connecting plate.

[0012] Furthermore, the plurality of oxygen-distributing membrane threads are equidistantly distributed in a circular shape, and the minimum distances between all the oxygen-distributing membrane threads and the inner side of the tank body are equal, so as to improve the uniformity of the oxygen-distributing membrane threads in transporting oxygen-containing culture fluid into the tank body.

[0013] Furthermore, the rotating assembly includes: A one-way disk is connected in a limited sliding manner in the connecting disk. The one-way disk is used to block the connection between the dissolved oxygen cylinder and the connecting disk. A tension spring is fixedly connected between the one-way disk and the dissolved oxygen cylinder. The one-way disk is sealingly and rotatably connected to a power rod. A one-way structure is provided between the one-way disk and the power rod. The power rod is provided with a spiral groove. The dissolved oxygen cylinder slides in a limited manner with the power rod through the spiral groove.

[0014] Furthermore, it also includes: The air pressure sensor is installed in the air delivery pipe and is used to monitor the air pressure in the air delivery pipe and the dissolved oxygen cylinder.

[0015] The beneficial effect produced by adopting the above technical scheme is that: the present invention transfers a part of the culture solution in the tank body to the dissolved oxygen cylinder, separates the culture solution in the dissolved oxygen cylinder from the culture solution in the tank body, and introduces oxygen into the dissolved oxygen cylinder, thereby increasing the dissolved oxygen concentration of the culture solution in the tank body without disturbing the culture solution in the tank body, thereby ensuring the normal growth and feeding of the bell worm and ensuring the reproduction rate of the bell worm.

[0016] The distribution plate is used to increase the contact area between oxygen and the culture solution in the oxygen dissolving cylinder, and the stirring piece is used to increase the degree of disorder of oxygen in the culture solution, thereby further increasing the contact area between oxygen and the culture solution in the oxygen dissolving cylinder, thereby increasing the rate at which oxygen is dissolved in the culture solution in the oxygen dissolving cylinder.

[0017] The high-oxygen culture solution in the dissolved oxygen cylinder is transported to various places in the tank body by using a connecting plate, so that the dissolved oxygen concentration in the culture solution in various places in the tank body is steadily increased, and the dissolved oxygen concentration in the culture solution in various places in the tank body is kept uniform, thereby preventing the bell worms from agglomerating due to uneven distribution of dissolved oxygen concentration in the culture solution, so that the bell worms can grow and reproduce normally. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention; Figure 2 It is a schematic diagram of the three-dimensional structure of the floating ring, the mounting shell and the air guide pump of the present invention; Figure 3 It is a three-dimensional structural cross-sectional view of the cover body, floating ring and mounting shell of the present invention; Figure 4 A three-dimensional structural cross-sectional view of the mounting shell, the shielding member and the sealing ring of the present invention; Figure 5 It is a schematic diagram of the three-dimensional structure of the gas transmission pipe and the distribution plate of the present invention; Figure 6 It is a three-dimensional structural cross-sectional view of the dissolved oxygen cylinder, the gas transmission pipe and the connecting plate of the present invention; Figure 7 The present invention is attached Figure 3 The enlarged view of point A in the middle; Figure 8 It is a schematic diagram of the three-dimensional structure of the one-way disk and the power rod of the present invention.

[0019] Explanation of the accompanying drawings: 1-tank body, 101-discharge port, 2-cover body, 201-feeding port, 202-expansion port, 3-floating ring, 4-mounting shell, 5-air pump, 6-dissolved oxygen cylinder, 601-power chamber, 602-liquid inlet hole, 603-exhaust hole, 7-gas pipe, 8-gas one-way valve, 9-shielding member, 10-limiting ring, 11-exhaust solenoid valve, 111-circulation hole, 12-distribution plate, 121-dissolved oxygen through hole, 122-power through hole, 13-stirring member, 14-sealing ring, 141-waste discharge channel, 142-waste extraction groove, 15-tee pipe, 16-oxygen extraction one-way valve, 17-carbon exhaust one-way valve, 18-connecting plate, 19-oxygen membrane wire, 20-one-way plate, 21-power rod, 211-spiral groove, 22-air pressure sensor. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] A microbial sterile culture device, please refer to Figure 1-Figure 5 , comprising: a tank body 1, a cover body 2 is fixedly connected to the upper side of the tank body 1; a floating ring 3, which is slidably connected to the tank body 1, and a mounting shell 4 is fixedly connected to the floating ring 3, and an air pump 5 is installed in the mounting shell 4, and a dissolved oxygen cylinder 6 is sealed and slidably connected in the mounting shell 4, and a tension spring is fixedly connected between the above two, and the mounting shell 4 and the dissolved oxygen cylinder 6 jointly form a power chamber 601, the air outlet pipe of the air pump 5 is connected to the power chamber 601, the lower part of the dissolved oxygen cylinder 6 is provided with a liquid inlet hole 602 for allowing the culture solution to enter therein, the upper part of the dissolved oxygen cylinder 6 is provided with an exhaust hole 603, and the mounting shell 4 is provided with a connecting hole that connects the power chamber 601 with the inside of the tank body 1; an air delivery pipe 7, which is fixedly connected to the dissolved oxygen cylinder 6 and is connected to the power chamber 601, and a gas delivery check valve 8 is installed in the air delivery pipe 7; a blocking component is arranged on the dissolved oxygen cylinder 6, and is used to block the liquid inlet hole 602 and the exhaust hole 603.

[0022] In the above scheme, it is intended to solve the problem that when the bell worm is cultivated in the prior art, oxygen is introduced into the culture solution to increase the concentration of dissolved oxygen in the culture solution, which affects the feeding of the bell worm. This scheme transfers a part of the culture solution in the tank body 1 to the dissolved oxygen cylinder 6, and separates the culture solution in the dissolved oxygen cylinder 6 from the culture solution in the tank body 1. In this way, the culture solution in the tank body 1 will not be disturbed during the process of introducing oxygen into the dissolved oxygen cylinder 6 to increase the dissolved oxygen concentration of the culture solution in the dissolved oxygen cylinder 6. Finally, the culture solution with high dissolved oxygen concentration in the dissolved oxygen cylinder 6 is re-added to the tank body 1 to increase the dissolved oxygen concentration of the entire culture solution in the tank body 1. In this way, the dissolved oxygen concentration of the culture solution in the tank body 1 is increased without disturbing the culture solution, thereby ensuring the normal growth and feeding of the bell worm and the reproduction rate of the bell worm.

[0023] The lower side of the tank body 1 is provided with a discharge port 101, and the cover body 2 is provided with a feed port 201 and a plurality of expansion ports 202, and initially, the feed port 201 and all the expansion ports 202 are in a blocked state; the bell worm will be fixed on the inner wall of the tank body 1 in the culture medium; the inner wall of the tank body 1 is provided with a limit strip for supporting the floating ring 3 (see attached Figure 2 ).

[0024] A solenoid valve can be installed in the discharge port 101 to control the opening and closing state of the discharge port 101; the feeding port 201 is used to feed culture fluid and bell worms into the tank body 1; the expansion port 202 is connected to the external oxygen supply device, and the oxygen supply device can automatically maintain the stability of the air pressure in the tank body 1; the force required to open the gas transmission check valve 8 is always greater than the tension of the tension spring between the mounting shell 4 and the dissolved oxygen cylinder 6; the number of liquid inlet holes 602 and exhaust holes 603 can be adjusted according to actual conditions. The number of liquid inlet holes 602 and exhaust holes 603 in this article is eight, and they are equidistantly distributed around the dissolved oxygen cylinder 6 in the circumferential direction; The gas supply pipe 7 is used to transport the oxygen in the power chamber 601 to the lower part of the dissolved oxygen cylinder 6; the gas supply one-way valve 8 is connected in the direction from top to bottom; a dissolved oxygen sensor (not shown in the figure) is installed at the bottom of the tank body 1 to monitor the concentration of dissolved oxygen in the culture medium and control the start and stop of the gas pump 5; the connecting hole of the mounting shell 4 is used to allow the gas in the power chamber 601 to slowly enter the tank body 1, but the flow rate of the gas through the connecting hole is less than the working efficiency of the gas pump 5. When the gas pump 5 is working, the influence of the connecting hole of the mounting shell 4 on the air pressure in the power chamber 601 can be ignored.

[0025] Please refer to Figure 3-Figure 5 The blocking component includes: a blocking member 9, which is limited and sealingly slidably connected to the dissolved oxygen cylinder 6 and is used to block the liquid inlet hole 602 and the exhaust hole 603; a limiting ring 10, which is fixed to the mounting shell 4, and the limiting ring 10 is used to support the blocking member 9.

[0026] In the above scheme, the purpose is to use the shielding member 9 to block the liquid inlet hole 602 and the exhaust hole 603, thereby separating the culture liquid in the dissolved oxygen cylinder 6 from the culture liquid in the tank body 1; there is a step portion on the upper part of the shielding member 9, the shielding member 9 is not in contact with the mounting shell 4, and the shielding member 9 itself has a greater weight than the friction between it and the dissolved oxygen cylinder 6; the inner diameter of the limiting ring 10 is smaller than the inner diameter of the mounting shell 4, and smaller than the maximum outer diameter of the stepped portion of the shielding member 9, and the limiting ring 10 is used to support the stepped portion of the shielding member 9. In this state, there is still a gap between the above two for gas circulation.

[0027] Please refer to Figure 3-Figure 5 , further comprising: an exhaust solenoid valve 11, installed on the upper side of the installation shell 4, the dissolved oxygen cylinder 6 is provided with a circulation hole 111, and the exhaust solenoid valve 11 is connected to the circulation hole 111 through a telescopic conduit.

[0028] In the above scheme, the exhaust solenoid valve 11 is used to control the connection between the interior of the dissolved oxygen cylinder 6 and the interior of the tank body 1, so that when the air pump 5 supplies oxygen to the dissolved oxygen cylinder 6, the excess oxygen that is not dissolved in the dissolved oxygen cylinder 6 can be discharged into the tank body 1 through the circulation hole 111 and the exhaust solenoid valve 11, thereby preventing the existence of excess oxygen in the dissolved oxygen cylinder 6 and increasing the pressure in the dissolved oxygen cylinder 6. Furthermore, when the dissolved oxygen cylinder 6 and the tank body 1 are reconnected, the high-oxygen culture solution in the dissolved oxygen cylinder 6 is prevented from causing a severe impact on the culture solution in the tank body 1, thereby maintaining the stability of the culture solution in the tank body 1.

[0029] Please refer to Figure 5 and Figure 6 , and also includes: a distribution plate 12, which is sealed and rotatably connected to the air supply pipe 7, the distribution plate 12 is connected to the air supply pipe 7, a side of the distribution plate 12 close to the air pump 5 is provided with a plurality of dissolved oxygen through holes 121 connected to the interior thereof, and a side of the distribution plate 12 away from the central axis thereof is provided with a plurality of power through holes 122 connected to the interior thereof; an agitator 13, fixedly connected to the distribution plate 12.

[0030] In the above scheme, the distribution plate 12 is used to increase the contact area between oxygen and the culture solution in the oxygen-dissolving cylinder 6, and the stirring member 13 is used to increase the degree of disorder of oxygen in the culture solution, thereby further increasing the contact area between oxygen and the culture solution in the oxygen-dissolving cylinder 6; the power through hole 122 is obliquely arranged on the distribution plate 12 (i.e., the extension line of the center line of the power through hole 122 does not pass through the central axis of the distribution plate 12), so that oxygen can provide a rotational force for the distribution plate 12 when it is ejected from the power through hole 122; there are fine through holes on the surface of the stirring member 13, which are used to allow the culture solution and oxygen bubbles to pass through the through holes of the stirring member 13, and the oxygen bubbles are "cut" by the gaps in the through holes of the stirring member 13, while the culture solution in the oxygen-dissolving cylinder 6 is in a flowing state, thereby increasing the contact efficiency between the culture solution and oxygen.

[0031] Please refer to Figure 3 , Figure 4 and Figure 7 , further comprising: a sealing ring 14, which is sealingly and slidably connected in the mounting shell 4, a spring is fixedly connected between the sealing ring 14 and the mounting shell 4, a waste discharge channel 141 is commonly provided in the mounting shell 4 and the floating ring 3, the waste discharge channel 141 is communicated with the power chamber 601, the sealing ring 14 is used to block the waste discharge channel 141, the floating ring 3 is provided with a waste extraction groove 142, the waste extraction groove 142 is communicated with the waste discharge channel 141; a three-way pipe 15, which is fixedly connected to the air pump 5 and communicated with the air inlet pipe of the air pump 5, an oxygen extraction check valve 16 is installed in one outlet of the three-way pipe 15, and a carbon exhaust check valve 17 connected to the outside is installed on the cover body 2, and the carbon exhaust check valve 17 is communicated with the other outlet of the three-way pipe 15 through a telescopic guide tube.

[0032] In the above scheme, the purpose is to use the waste discharge channel 141, the power chamber 601, the air pump 5, the three-way pipe 15 and the carbon exhaust check valve 17 to discharge the carbon dioxide generated during the cultivation of the bell worm; the waste extraction tank 142 is located at the lower part of the floating ring 3, which is convenient for discharging the carbon dioxide in the tank body 1 to the outside (because the density of carbon dioxide is greater than the density of oxygen, carbon dioxide will gather near the liquid surface); when the air pump 5 transports gas from the tank body 1 to the power chamber 601, the oxygen extraction check valve 16 is opened, the carbon exhaust check valve 17 is closed, the oxygen extraction check valve 16 is connected in the direction from right to left, and the carbon exhaust check valve 17 is connected in the direction from bottom to top.

[0033] The working principle of the above scheme is as follows: when cultivating bell worms, sterilized culture solution and disinfected bell worms are sequentially added into the tank body 1 through the feeding port 201 (in this process, the culture solution in the tank body 1 contacts the floating ring 3, and the floating ring 3 is moved upward by the buoyancy, and finally the culture solution can cause the floating ring 3 to lose contact with the limit bar through the buoyancy, so that the buoyancy of the floating ring 3 is balanced with the gravity of the parts on it), and then the oxygen supply device is connected to any expansion port 202 (the other expansion ports 202 remain blocked), and the cultivation of bell worms begins; when the dissolved oxygen sensor detects that the dissolved oxygen concentration in the culture solution is in a low range (that is, the dissolved oxygen concentration is close to 3 mg / L), the air pump 5 is started, and the air pump 5 first extracts the gas in the power chamber 601 and transports it to the outside, at which time the carbon exhaust check valve 17 is opened, so that the power chamber 601 is opened. The air pressure in the cavity 601 decreases, and a pressure difference is generated on the upper and lower sides of the sealing ring 14, so that the sealing ring 14 moves up and compresses the adjacent spring, and the sealing ring 14 releases the blockage of the waste discharge channel 141. At this time, the gas close to the liquid surface of the culture solution in the tank body 1 flows into the power cavity 601 through the waste extraction groove 142 and the waste discharge channel 141, and is discharged to the outside through the air pump 5, the three-way pipe 15 and the carbon exhaust check valve 17. During this process, the oxygen supply device continuously supplies oxygen to the tank body 1 and maintains the stability of the air pressure in the tank body 1; after a certain period of extraction (the certain period of time here is determined according to the extraction rate of the air pump 5 and the rate at which the bell worm produces carbon dioxide, aiming to minimize the content of carbon dioxide in the tank body 1), the air pump 5 switches the suction state and starts to transport the gas in the tank body 1 to the power cavity 601, and the exhaust solenoid valve 11 is opened.

[0034] During the process of the air pump 5 supplying air to the power chamber 601, the carbon exhaust check valve 17 is closed, and the sealing ring 14 moves downward and resets under the action of the adjacent spring, and the waste discharge channel 141 is blocked again; the oxygen extraction check valve 16 is opened, and the gas in the tank body 1 enters the power chamber 601 through the oxygen extraction check valve 16, the three-way pipe 15 and the air pump 5, so that the air pressure in the power chamber 601 increases, and the gas in the power chamber 601 pushes the dissolved oxygen cylinder 6 to move downward, and stretches the tension spring adjacent to the dissolved oxygen cylinder 6, and the dissolved oxygen cylinder 6 drives the shielding member 9 to move downward. As the dissolved oxygen cylinder 6 moves downward, the dissolved oxygen cylinder 6 gradually contacts with the culture solution, and the culture solution passes through the liquid inlet hole 602 enters the dissolved oxygen cylinder 6, and at the same time, the original gas in the dissolved oxygen cylinder 6 is discharged into the mounting shell 4 through the exhaust hole 603 (the gas entering the mounting shell 4 is discharged from the mounting shell 4 through the gap between the shielding member 9 and the mounting shell 4 and the gap between the shielding member 9 and the limiting ring 10), and the distance between the stepped portion of the shielding member 9 and the limiting ring 10 gradually decreases, and finally the stepped portion of the shielding member 9 contacts the upper side of the limiting ring 10 and stops moving downward, and the dissolved oxygen cylinder 6 continues to move downward, so that the liquid inlet hole 602 and the exhaust hole 603 are gradually blocked by the shielding member 9, and finally the dissolved oxygen cylinder 6 contacts the upper side of the shielding member 9 and stops moving downward.

[0035] After the dissolved oxygen cylinder 6 stops moving downward, as the air pump 5 continues to supply air to the power chamber 601, the air pressure in the power chamber 601 continues to increase until the air pressure in the power chamber 601 pushes the gas supply one-way valve 8 to open. At this time, the gas in the power chamber 601 is transported to the distribution plate 12 through the gas supply one-way valve 8 and the gas supply pipe 7, and is discharged through the dissolved oxygen through hole 121 and the power through hole 122. When the gas is discharged through the power through hole 122, the gas gives a reaction force to the distribution plate 12 to rotate, so that the distribution plate 12 drives the agitator 13 to rotate, thereby improving the uniformity of the contact between the culture solution and the gas at various locations.

[0036] The gas entering the dissolved oxygen cylinder 6 is not completely dissolved in the culture solution. At this time, the excess gas is discharged into the tank body 1 through the circulation hole 111, the telescopic guide tube and the exhaust solenoid valve 11. After repeating for a period of time (the period of time here is determined according to the current dissolved oxygen concentration in the tank body 1 and the volume ratio of the culture solution in the dissolved oxygen cylinder 6 to the culture solution in the tank body 1, so that after the high-oxygen culture solution in the dissolved oxygen cylinder 6 enters the tank body 1, the dissolved oxygen concentration of the culture solution in the tank body 1 can be close to 5 mg / L), the air pump 5 is stopped and the exhaust solenoid valve 11 is closed. At this time, the gas supply one-way valve 8 is closed, and the gas in the power chamber 601 is The oxygen cylinder 6 flows into the tank body 1 through the connecting hole of the mounting shell 4, and the dissolved oxygen cylinder 6 moves upward under the action of its adjacent tension spring, and the shielding member 9 temporarily stops under the action of gravity. As the dissolved oxygen cylinder 6 moves upward, the shielding member 9 gradually releases the blockage of the liquid inlet hole 602 and the exhaust hole 603. At this time, the high-oxygen culture solution in the dissolved oxygen cylinder 6 is discharged into the tank body 1 through the liquid inlet hole 602. Since the upward movement speed of the dissolved oxygen cylinder 6 is related to the flow area of ​​the connecting hole on the mounting shell 4, the dissolved oxygen cylinder 6 moves upward slowly, and then the high-oxygen culture solution flowing out of the dissolved oxygen cylinder 6 slowly merges into the tank body 1. In this process, the disturbance to the culture solution in the tank body 1 is small.

[0037] After the shielding member 9 releases the blockage of the liquid inlet hole 602 and the exhaust hole 603, the dissolved oxygen cylinder 6 drives the shielding member 9 to move upward until the dissolved oxygen cylinder 6 and the shielding member 9 are both reset. At this point, the adjacent tension spring of the dissolved oxygen cylinder 6 is reset, completing the single oxygenation work of the culture solution in the tank body 1. Thereafter, the dissolved oxygen sensor in the tank body 1 monitors the dissolved oxygen concentration of the culture solution in the tank body 1 in real time, and maintains the dissolved oxygen concentration of the culture solution in the tank body 1 between 5 mg / L to ensure the normal growth and reproduction of the bell worm. Finally, after the cultivation of the bell worm is completed, the bell worm and the culture solution are discharged together through the discharge port 101.

[0038] Please refer to Figure 2-Figure 6 and Figure 8, further comprising: a connecting disk 18, which is sealed and rotatably connected to the lower side of the dissolved oxygen cylinder 6, the connecting disk 18 is fixedly connected and connected to a plurality of oxygen-distributing membrane wires 19, and the oxygen-distributing membrane wires 19 are connected to the inside of the dissolved oxygen cylinder 6 through the connecting disk 18; a rotating component, which is arranged in the connecting disk 18, and is used to control the flow direction of the culture solution between the dissolved oxygen cylinder 6 and the connecting disk 18; the plurality of oxygen-distributing membrane wires 19 are distributed in an annular shape with equal spacing, and the minimum distances between all the oxygen-distributing membrane wires 19 and the inner side of the tank body 1 are equal, which is used to improve the uniformity of the oxygen-distributing membrane wires 19 in transporting the high-oxygen culture solution into the tank body 1; a rotating component The component includes: a one-way disk 20, which is limitedly slidably connected in the connecting disk 18, the one-way disk 20 is used to block the connection between the dissolved oxygen cylinder 6 and the connecting disk 18, a tension spring is fixedly connected between the one-way disk 20 and the dissolved oxygen cylinder 6, the one-way disk 20 is sealed and rotatably connected to the power rod 21, a one-way structure is provided between the one-way disk 20 and the power rod 21, the power rod 21 is provided with a spiral groove 211, and the dissolved oxygen cylinder 6 is limitedly slidable with the power rod 21 through the spiral groove 211; an air pressure sensor 22 is installed in the gas pipeline 7, and is used to monitor the air pressure in the gas pipeline 7 and the dissolved oxygen cylinder 6.

[0039] In the above scheme, the purpose is to use the connecting plate 18 to transport the high-oxygen culture solution in the dissolved oxygen cylinder 6 to various places in the tank body 1, so that the dissolved oxygen concentration in the culture solution in various places in the tank body 1 can be stably increased, and the dissolved oxygen concentration in the culture solution in various places in the tank body 1 can be kept uniform, thereby preventing the bell worms from agglomerating due to the uneven distribution of dissolved oxygen concentration in the culture solution (if the bell worms agglomerate, food competition will intensify, excrement waste will accumulate and movement will be restricted, which is not conducive to the growth and reproduction of the bell worms); the oxygen-bearing membrane wire 19 is a microfiltration membrane wire, which can slowly seep out through the micropores thereon when there is a high-pressure fluid in the oxygen-bearing membrane wire 19, but the diameter of the micropores on the oxygen-bearing membrane wire 19 is larger than the diameter of the nutrients and the bell worms in the culture solution; all the bending parts of the lower part of the oxygen-bearing membrane wire 19 are provided with a metal skeleton, which is used to enable all the oxygen-bearing membrane wires 19 to roughly form a cylindrical shape (the oxygen-bearing membrane wires in the attached figure The shape and thickness of 19 are for illustration only. In actual use, the middle parts of all oxygen-distributing membrane wires 19 overlap each other), and the minimum distance between the oxygen-distributing membrane wires 19 and the side wall of the tank body 1 is equal to the minimum distance between the oxygen-distributing membrane wires 19 and the bottom of the tank body 1 when the dissolved oxygen cylinder 6 moves downward to the limit position (that is, the dissolved oxygen cylinder 6 contacts the upper side of the shielding member 9); since the bell worm will be fixed on the inner wall of the tank body 1 in the culture solution, the oxygen-distributing membrane wires 19 are kept at the same distance from the side wall and the bottom of the tank body 1, so that the high-oxygen culture solution can be more evenly transported to the position close to the bell worm, so that the dissolved oxygen concentration of the culture solution contacted by the bell worms located in various places in the tank body 1 remains uniform, and the bell worms are prevented from agglomerating; the one-way structure between the one-way disk 20 and the power rod 21 can be a ratchet pawl structure, and the power rod 21 can drive the one-way disk 20 to rotate together when it rotates clockwise (the rotation perspective in this article is from top to bottom).

[0040] The dissolved oxygen cylinder 6, the connecting disk 18, the one-way disk 20 and the power rod 21 form a structure similar to a one-way valve, which limits the culture solution to flow only from the dissolved oxygen cylinder 6 to the connecting disk 18. When the one-way disk 20 moves downward due to the pressure in the dissolved oxygen cylinder 6, the one-way disk 20 drives the power rod 21 to move downward, and the dissolved oxygen cylinder 6 squeezes the power rod 21 through the spiral groove 211, causing the power rod 21 to rotate clockwise, and then the power rod 21 drives the one-way disk 20, the connecting disk 18 and all the oxygen distribution membrane wires 19 to rotate, so that each time the oxygenation work is performed, the oxygen distribution membrane wires 19 are located at different positions in the tank body 1, thereby improving the uniformity of the oxygen distribution membrane wires 19 in transporting oxygen-containing culture solution into the tank body 1.

[0041] The working principle of the above scheme is as follows: when the dissolved oxygen sensor detects that the dissolved oxygen concentration in the culture solution is low, the steps of starting the air pump 5 to discharge the carbon dioxide in the tank body 1 and moving the dissolved oxygen cylinder 6 downward to allow oxygen to circulate in the dissolved oxygen cylinder 6 are repeated. During this process, the dissolved oxygen cylinder 6 drives the connecting disk 18 and the oxygen membrane wire 19 to move downward together. When the dissolved oxygen concentration in the dissolved oxygen cylinder 6 reaches the optimal level (that is, after the oxygen in the dissolved oxygen cylinder 6 circulates for a period of time), the exhaust solenoid valve 11 is closed to increase the air pressure in the power chamber 601 and the dissolved oxygen cylinder 6 until the pressure in the dissolved oxygen cylinder 6 can squeeze the one-way disk 20 to move downward. At this time, the one-way disk 20 drives the power rod 21 to move downward, and the power rod 21 is in the spiral groove 2 11, it rotates clockwise, and the power rod 21 drives the connecting disk 18 and the oxygen-bearing membrane wire 19 to rotate through the one-way disk 20, so that the oxygen-bearing membrane wire 19 is offset clockwise relative to the position during the last oxygenation operation. At this time, the one-way disk 20 releases the blockage of the connection between the dissolved oxygen cylinder 6 and the connecting disk 18, and stretches the tension spring adjacent to the one-way disk 20, so that the high-oxygen culture solution in the dissolved oxygen cylinder 6 enters the connecting disk 18. Under the action of the pressure in the dissolved oxygen cylinder 6, the high-oxygen culture solution in the connecting disk 18 enters the oxygen-bearing membrane wire 19, penetrates outward at various positions of the oxygen-bearing membrane wire 19, and finally passes through the oxygen-bearing membrane wire 19 and merges into the culture solution in the tank body 1, so that the dissolved oxygen concentration of the culture solution in various places in the tank body 1 is synchronously increased.

[0042] During the process of high-oxygen culture solution entering the tank body 1 through the oxygen-bearing membrane wire 19, the air pressure sensor 22 continuously monitors the air pressure in the dissolved oxygen cylinder 6 and the air pipe 7, and controls the working power of the air pump 5, so that the air pressure in the dissolved oxygen cylinder 6 and the air pipe 7 remains consistent during multiple oxygenation operations, thereby making the high-oxygen culture solution pass through the oxygen-bearing membrane wire 19 at a consistent rate, preventing some micropores on the oxygen-bearing membrane wire 19 from being blocked, which increases the speed of the culture solution passing through the remaining micropores of the oxygen-bearing membrane wire 19, resulting in a large disturbance when the high-oxygen culture solution contacts the culture solution in the tank body 1 after passing through the oxygen-bearing membrane wire 19, thereby allowing the bell worm to eat normally.

[0043] As the high-oxygen culture solution in the dissolved oxygen cylinder 6 enters the tank body 1 through the connecting disk 18 and the oxygen-distributing membrane wire 19, after the high-oxygen culture solution in the dissolved oxygen cylinder 6 completely enters the connecting disk 18, the air pump 5 is stopped, so that the one-way disk 20 moves up under the action of its adjacent tension spring, and the one-way disk 20 drives the power rod 21 to move up. The power rod 21 rotates counterclockwise under the action of the spiral groove 211. At this time, the power rod 21 cannot drive the one-way disk 20 to rotate until the one-way disk 20 is reset. At this time, the one-way disk 20 re-blocks the connection between the dissolved oxygen cylinder 6 and the connecting disk 18, and then repeats the above-mentioned steps of discharging the gas in the power chamber 601 through the connecting hole of the mounting shell 4, and the dissolved oxygen cylinder 6 drives the connecting disk 18 and the oxygen-distributing membrane wire 19 to move up and reset together.

[0044] While the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that many changes, modifications, substitutions and variations can be made to the embodiments without departing from the principles and spirit of the invention.

Claims

1. A microbial aseptic culture device, comprising: A tank body (1), wherein a cover body (2) is fixedly connected to the upper side of the tank body (1); a floating ring (3) slidably connected to the tank body (1); the floating ring (3) is fixedly connected to a mounting shell (4); an air pump (5) is installed on the mounting shell (4); an oxygen dissolving cylinder (6) is sealingly and slidably connected to the mounting shell (4); a tension spring is fixedly connected between the mounting shell (4) and the oxygen dissolving cylinder (6); the mounting shell (4) and the oxygen dissolving cylinder (6) together form a power chamber (601); an air outlet pipe of the air pump (5) is connected to the power chamber (601); a liquid inlet hole (602) for allowing a culture fluid to enter the dissolved oxygen cylinder (6) is provided at the bottom of the dissolved oxygen cylinder (6); an air outlet hole (603) is provided at the top of the dissolved oxygen cylinder (6); and a connecting hole for connecting the power chamber (601) with the inside of the tank body (1) is provided on the mounting shell (4); An air delivery pipe (7) is fixedly connected to the dissolved oxygen cylinder (6) and communicated with the power chamber (601); a gas delivery one-way valve (8) is installed in the air delivery pipe (7); A blocking component is arranged on the dissolved oxygen cylinder (6) and is used to block the liquid inlet hole (602) and the exhaust hole (603).

2. A microbial aseptic culture device according to claim 1, characterized in that: The blocking component comprises: A shielding member (9) is connected to the dissolved oxygen cylinder (6) in a position-limiting and sealing manner and is used to block the liquid inlet hole (602) and the exhaust hole (603); A limiting ring (10) is fixedly connected to the mounting shell (4), and the limiting ring (10) is used to support the shielding member (9).

3. A microbial aseptic culture device according to claim 2, characterized in that include: An exhaust solenoid valve (11) is installed on the upper side of the installation shell (4); the dissolved oxygen cylinder (6) is provided with a circulation hole (111); and the exhaust solenoid valve (11) is connected to the circulation hole (111) via a telescopic conduit.

4. A microbial aseptic culture device according to claim 1, characterized in that include: A distribution plate (12) is sealingly rotatably connected to the gas delivery pipe (7), the distribution plate (12) being in communication with the gas delivery pipe (7), a side of the distribution plate (12) close to the gas guide pump (5) being provided with a plurality of dissolved oxygen through holes (121) in communication with the interior thereof, and a side of the distribution plate (12) away from the central axis thereof being provided with a plurality of power through holes (122) in communication with the interior thereof.

5. A microbial aseptic culture device according to claim 4, characterized in that include: The stirring member (13) is fixedly connected to the distribution plate (12).

6. A microbial aseptic culture device according to claim 3, characterized in that include: a sealing ring (14) sealingly and slidably connected in the mounting shell (4); a spring is fixedly connected between the sealing ring (14) and the mounting shell (4); a waste discharge channel (141) is provided in both the mounting shell (4) and the floating ring (3); the waste discharge channel (141) is communicated with the power chamber (601); the sealing ring (14) is used to block the waste discharge channel (141); the floating ring (3) is provided with a waste extraction groove (142); the waste extraction groove (142) is communicated with the waste discharge channel (141); A three-way pipe (15) is fixedly connected to the air pump (5) and communicated with the air inlet pipe of the air pump (5); an oxygen extraction one-way valve (16) is installed in one outlet of the three-way pipe (15); a carbon exhaust one-way valve (17) communicated with the outside is installed on the cover body (2); and the carbon exhaust one-way valve (17) is communicated with the other outlet of the three-way pipe (15) through a telescopic conduit.

7. A microbial aseptic culture device according to claim 3, characterized in that include: A connecting plate (18) is sealingly and rotatably connected to the lower side of the dissolved oxygen cylinder (6); the connecting plate (18) is fixedly connected to and communicates with a plurality of oxygen distribution membrane wires (19); the oxygen distribution membrane wires (19) are communicated with the interior of the dissolved oxygen cylinder (6) through the connecting plate (18); A rotating assembly is arranged in the connecting plate (18) and is used to control the flow direction of the culture solution between the dissolved oxygen cylinder (6) and the connecting plate (18).

8. A microorganism aseptic culture device according to claim 7, characterized in that: The plurality of oxygen-distributing membrane threads (19) are distributed equidistantly in a ring shape, and the minimum distances between all the oxygen-distributing membrane threads (19) and the inner side of the tank body (1) are equal, so as to improve the uniformity of the oxygen-distributing membrane threads (19) in transporting the oxygen-containing culture fluid into the tank body (1).

9. A microorganism aseptic culture device according to claim 7, characterized in that: The rotating assembly comprises: A one-way disc (20) is connected in a limited sliding manner in the connection disc (18); the one-way disc (20) is used to block the connection between the dissolved oxygen cylinder (6) and the connection disc (18); a tension spring is fixedly connected between the one-way disc (20) and the dissolved oxygen cylinder (6); the one-way disc (20) is sealingly and rotatably connected to a power rod (21); a one-way structure is provided between the one-way disc (20) and the power rod (21); the power rod (21) is provided with a spiral groove (211); the dissolved oxygen cylinder (6) is limitedly slidable with the power rod (21) via the spiral groove (211).

10. A microorganism aseptic culture device according to claim 9, characterized in that include: An air pressure sensor (22) is installed in the air supply pipe (7) and is used to monitor the air pressure in the air supply pipe (7) and the dissolved oxygen cylinder (6).

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