Full-automatic cell culture device and use method thereof

Abstract

The invention discloses a full-automatic cell culture device and a use method thereof, and belongs to the technical field of cell culture.A sealing cover is tightly attached to the side wall of a culture box, a sliding plate is supported through the elastic force of a supporting spring, a clamping block is driven to be clamped into a clamping groove, and the purpose of locking the sealing cover is achieved; in the same way, erecting assemblies at other positions of the incubator are operated, then the culture test tubes are conveniently fed into the incubator, a sliding plate is pulled through a pull rod, the sliding plate drives a clamping block to be separated from a clamping groove, namely, the locking state between a sealing cover and the incubator is relieved, and each culture groove is independently designed; cells in different time periods and different types can be independently cultured, taken and placed, operation is convenient and fast, all independently-designed erecting assemblies are integrated in a single incubator, cell culture does not need to be carried out by additionally arranging multiple culture platforms, carrying and transferring are convenient, the use cost is reduced, and meanwhile the cell culture efficiency is improved. And cross contamination caused by conduction of a plurality of culture tanks can be prevented.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, in particular to a fully automatic cell culture device and a use method thereof. Background Art

[0002] Cell culture refers to all in vitro cultures. Cell culture is now widely used in biology, medicine, new drug research and development and other fields, becoming one of the most important basic sciences. Cell culture technology can transform a cell into a simple single cell or a few differentiated multi-cells through mass culture. Fully automatic cell culture is a method that uses advanced technology to fully automate the cell culture process. The development of this technology is of great significance to improving the efficiency and accuracy of cell culture and reducing errors caused by manual operations.

[0003] At present, fully automatic cell culture usually adopts an automated cell culture platform. Test tubes containing cells are added to the culture platform for culture. When the culture platform is opened to take and put the test tubes, all the test tubes containing cells will be exposed to the outside, causing cross contamination between the cells. When multiple culture platforms are added independently for cell culture, the cost of use will undoubtedly increase. At the same time, it is inconvenient to carry and transfer, and the workload will also increase when taking and putting the test tubes. Summary of the invention

[0004] The object of the present invention is to provide a fully automatic cell culture device and a method of using the same, so as to solve the problems raised in the above-mentioned background technology that when the culture platform is opened to take and place the test tubes, all the test tubes containing cells will be exposed to the outside, causing cross contamination between the cells; when multiple culture platforms are independently added for cell culture, the use cost will undoubtedly increase, and it will be inconvenient to carry and transfer, and the workload will also increase when taking and placing the test tubes.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] A fully automatic cell culture device comprises an incubator, wherein a plurality of culture troughs are provided outside the incubator, a mounting assembly is installed in the culture trough, a sealing cover is fixedly connected to a side of the mounting assembly away from the incubator, the sealing cover seals the opening of the culture trough, a plurality of culture test tubes are installed in the mounting assembly, a slide groove is provided at the top and bottom of the sealing cover respectively, a locking assembly is slidably connected in the slide groove, a handle is fixed at one end of two locking assemblies close to each other respectively, a plurality of card grooves are provided above and below the outer wall of the incubator respectively, the ends of the locking assemblies are carded in the card grooves, an automated culture analyzer is installed at the top of the incubator, the ends of the automated culture analyzer pass through and are connected in the plurality of culture troughs, a central groove is provided in the middle of the incubator, the bottom of the automated culture analyzer is installed in the central groove, four grooves are provided at the bottom of the incubator, and support assemblies are installed in the four grooves.

[0007] As a further solution of the present invention, the outer wall of the culture box is provided with a plurality of light-transmitting grooves, the inner walls of the light-transmitting grooves are transparent, the light-transmitting grooves are located between two adjacent culture grooves, and a sealing plate is installed at the opening of the light-transmitting grooves.

[0008] As a further solution of the present invention, a collecting bin is provided at the lower part of the culture box, a solenoid valve is installed on the top of the inner wall of the collecting bin, the solenoid valve is installed through the bottom of the inner wall of the culture tank, and a main discharge valve is installed on the bottom of the inner wall of the collecting bin.

[0009] As a further solution of the present invention, the mounting assembly includes a column, a horizontal plate and a support plate are fixedly connected between the column and the sealing cover, two limit partitions are arranged between the support plate and the horizontal plate, the limit partitions are installed on the opposite sides of the column and the sealing cover, a plurality of clamping holes are provided on the horizontal plate, a plurality of ball grooves and leakage holes are provided on the support plate, the bottom end of the culture test tube passes through the clamping holes and the limit partitions and is located in the ball groove, and the column, the horizontal plate and the support plate are slidably connected in the culture groove.

[0010] As a further solution of the present invention, the locking assembly includes a slide plate, which is slidably connected in a slide groove, and a clamping block is fixedly connected to the slide plate, and the clamping block is clamped in the clamping groove, one side of the clamping block is designed as an arc surface and the other side is designed as a flat surface, and a pull rod is fixed inside the slide plate, the end of the pull rod is fixed to the handle, and a support spring is connected to the outer sleeve of the pull rod, and the support spring is fixed between the sealing cover and the inner wall of the slide plate.

[0011] As a further solution of the present invention, the automated culture analyzer includes an automated control module, a fixed column is installed in the middle of the automated control module, the fixed column is installed in the center groove, a ferrule is provided on the outside of the automated control module, the ferrule is connected to the top of the incubator, and a group of online observers are respectively provided at the bottom of the automated control module corresponding to the positions of several culture grooves, each group of online observers has five online observers, and each online observer is located directly above the culture tube.

[0012] As a further solution of the present invention, the support assembly includes a fixing ring, four support rods are fixed on the fixing ring, universal wheels are installed at the bottom ends of the support rods, the top ends of the support rods are slidably connected in the grooves, buffer springs are connected to the outer sleeves of the support rods, the buffer springs are fixed between the bottom of the incubator and the fixing ring, and damping springs are fixed at the top ends of the support rods, and the damping springs are installed in the grooves.

[0013] A method for using a fully automatic cell culture device, the method comprising the following steps:

[0014] When cells are fully automatically cultured, a culture tube containing cells is placed in the mounting assembly, so that the bottom end of the culture tube passes through the clamp hole and the limiting partition and is located in the ball groove, the culture tube is limited by the ball groove and the clamp hole, and the limiting partition is used to support it, so as to ensure the stability of the culture tube mounting, and the top of the culture tube is higher than the clamp hole. After the placement of the culture tube is completed, the sealing cover is pushed, so that the sealing cover drives the culture tube to move through the horizontal plate and the supporting plate, so that the culture tube can enter the culture groove in the incubator;

[0015] During the movement of the sealing cover, the locking assembly will be driven to approach the incubator. When the card block in the locking assembly contacts the side wall of the incubator, because the side of the card block close to the incubator is designed as an arc surface, the card block will be pressed to drive the slide plate to slide into the slide groove. When the sealing cover is close to the side wall of the incubator, the slide plate is supported by the elastic force of the supporting spring, so that the slide plate drives the card block to be stuck in the card groove, thereby achieving the purpose of locking the sealing cover, so that the sealing cover can seal the opening of the culture tank to prevent external dust and impurities from entering the culture tank. A mounting assembly is installed in each culture tank, and the mounting assembly at other positions of the incubator is operated in the same way, so as to facilitate the delivery of the culture tube into the incubator. Moreover, each culture tank is independently designed, and can independently culture and take and place cells of different time periods and different types. All independently designed mounting assemblies are integrated in a single incubator to perform fully automatic culture of cells in the culture tube.

[0016] When the cells in the culture tube are cultured through the incubator, the bottom end of the online observer in the automated culture analyzer is connected to the culture tank, and each online observer is located directly above the culture tube. The cells in the culture tube are observed online through the online observer and the signal is transmitted to the automated control module. The automated culture analyzer can analyze the real-time data. When the culture tube in the incubator needs to be light-transmitted, the sealing plate at the opening of the light-transmitting tank can be opened. Since the inner wall of the light-transmitting tank is designed to be transparent, the light-transmitting tank is located between two adjacent culture tanks, and the culture tube can be observed and light-transmitted through the light-transmitting tank. The sealing plate can be inserted into the opening of the light-transmitting tank to avoid light.

[0017] When the culture tube is damaged or liquid overflows, the liquid will flow along the culture tank through the leak hole and into the solenoid valve below. When the solenoid valve is opened, the liquid will be introduced into the collection chamber, so that the collection chamber can collect the liquid in all the culture tanks and discharge it from the incubator by opening the main discharge valve at the bottom. Each solenoid valve is designed independently to prevent cross contamination caused by the connection of multiple culture tanks, thereby affecting the cell culture work.

[0018] When transferring the culture device, the culture box is pushed to make the universal wheel in the support assembly roll on the ground. During the movement of the culture box, the universal wheel will squeeze the support rod, and the support rod will squeeze the buffer spring through the fixed ring. After the buffer spring is compressed, it will provide a reverse force to the fixed ring, which can play a role in shock absorption of the culture box. The damping spring in the groove supports the end of the support rod to prevent the incubator from vibrating too much and affecting the stability of the culture tube.

[0019] When taking out the culture tube, by squeezing the two handles, the two handles respectively pull the slide plate through the pull rod, so that the slide plate drives the card block to disengage from the card slot, that is, the locking state between the sealing cover and the incubator is released, and the culture tube can be taken out of the culture tank by pulling the handles outward, and the entire mounting assembly and the culture tube can be completely moved out of the culture tank, thereby facilitating the taking and placing of the culture tube. When removing the automated culture analyzer, hold the card sleeve and pull it upward, so that the card sleeve drives the fixed column to slide in the center slot through the automated control module, and the automated control module drives multiple online observers to move out of the incubator, and the automated culture analyzer can be removed for daily cleaning and maintenance of the device.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. The present invention brings the locking assembly close to the incubator, and when the sealing cover is close to the side wall of the incubator, the slide plate is supported by the elastic force of the supporting spring, so that the slide plate drives the card block to be stuck in the card slot, thereby achieving the purpose of locking the sealing cover, so that the sealing cover can seal the opening of the culture tank to prevent external dust and impurities from entering the culture tank. Each culture tank is installed with a mounting assembly, and the mounting assemblies at other positions of the incubator are operated in the same way, so as to facilitate the delivery of the culture tube into the incubator. When taking out the culture tube, the two handles are squeezed so that the two handles respectively pull the slide plate through the pull rod, so that the slide plate drives the card block to be separated from the card slot, that is, the locking state between the sealing cover and the incubator is released, and each culture tank is independently designed, and cells of different time periods and different types can be independently cultured and taken and placed, and the operation is convenient. All independently designed mounting assemblies are integrated in a single incubator, and there is no need to add multiple culture platforms for cell culture, and it is convenient to carry and transfer, while reducing the use cost, it can also prevent cross contamination caused by the connection of multiple culture tanks.

[0022] 2. The present invention places a culture tube filled with cells in a mounting assembly so that the bottom end of the culture tube passes through the card hole and the limiting partition and is located in the ball groove. The culture tube is limited by the ball groove and the card hole, and supported by the limiting partition to ensure the stability of the culture tube mounting. The top of the culture tube is higher than the card hole, which is convenient for taking and placing the culture tube. When the culture tube is damaged or liquid overflows, the liquid will flow along the culture groove and through the leakage hole into the electromagnetic valve below. After the electromagnetic valve is opened, the liquid will be introduced into the collection bin, so that the collection bin can collect the liquid in all the culture grooves and can discharge the culture box by opening the bottom main discharge valve. Each electromagnetic valve is independently designed to prevent cross contamination caused by the connection of multiple culture grooves, thereby affecting the cell culture work.

[0023] 3. By squeezing the two handles, the slide plate drives the card block to disengage from the card slot, and the handles can be pulled outward to bring the culture tube out of the culture tank, and the entire mounting assembly and the culture tube can be completely moved out of the culture tank, thereby facilitating the taking and placing of the culture tube. When removing the automated culture analyzer, hold the card sleeve and pull it upward, so that the card sleeve drives the fixed column to slide in the center slot through the automated control module, and the automated control module drives multiple online observers to move out of the incubator, and the automated culture analyzer can be removed. Therefore, the device adopts a modular design method to enable each component to be disassembled and reassembled, so as to facilitate daily cleaning and maintenance of the device, making the device widely applicable. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings required for describing the embodiments will be briefly introduced below. Obviously, the accompanying drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without paying creative work.

[0025] Figure 1 It is a three-dimensional structural schematic diagram of the present invention;

[0026] Figure 2 It is a schematic diagram of the structure of the present invention;

[0027] Figure 3 It is a structural schematic diagram of the connection between the incubator of the present invention and the automated culture analyzer;

[0028] Figure 4 It is a structural schematic diagram of the incubator of the present invention;

[0029] Figure 5 It is a structural schematic diagram of a partial cross section of the incubator of the present invention;

[0030] Figure 6 It is a schematic diagram of the structure of the incubator of the present invention when viewed from above;

[0031] Figure 7 It is a structural schematic diagram of the support assembly of the present invention;

[0032] Figure 8 For the present invention Figure 7 The enlarged structural diagram at A in the middle;

[0033] Fig. 9 It is a structural schematic diagram of the erection assembly of the present invention;

[0034] Fig.10 It is a structural schematic diagram of the cross section of the locking assembly of the present invention.

[0035] In the accompanying drawings, the components represented by the reference numerals are listed as follows:

[0036] 1. Incubator; 2. Incubator tank; 3. Mounting assembly; 301. Column; 302. Clamp hole; 303. Horizontal plate; 304. Limiting partition; 305. Support plate; 306. Ball slot; 307. Leak hole; 4. Sealing cover; 5. Incubator tube; 6. Slide; 7. Locking assembly; 701. Slide plate; 702. Clamp block; 703. Pull rod; 704. Support spring; 8. Handle; 9. Clamp slot; 10. Automation Culture analyzer; 101, automation control module; 102, fixed column; 103, ferrule; 104, online observer; 11, center slot; 12, groove; 13, support assembly; 131, fixed ring; 132, support rod; 133, universal wheel; 134, buffer spring; 135, damping spring; 14, light-transmitting slot; 15, sealing plate; 16, collection bin; 17, solenoid valve; 18, main discharge valve. DETAILED DESCRIPTION

[0037] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] See also Figure 1-Figure 10 The present invention provides a technical solution: a fully automatic cell culture device, comprising a culture box 1, a plurality of culture tanks 2 are provided outside the culture box 1, a mounting assembly 3 is installed in the culture tank 2, a sealing cover 4 is fixedly connected to the side of the mounting assembly 3 away from the culture box 1, the sealing cover 4 seals the opening of the culture tank 2, a plurality of culture test tubes 5 are installed in the mounting assembly 3, a slide groove 6 is provided at the top and bottom of the sealing cover 4, a locking assembly 7 is slidably connected in the slide groove 6, a handle 8 is fixed at one end of the two locking assemblies 7 close to each other, a plurality of card slots 9 are provided above and below the outer wall of the culture box 1, and the ends of the locking assembly 7 are carded in the card slots 9 for locking. An automated culture analyzer 10 is installed at the top of the culture box 1, and the ends of the automated culture analyzer 10 penetrate and communicate with the plurality of culture tanks 2, a central groove 11 is provided in the middle of the culture box 1, and the bottom of the automated culture analyzer 10 is installed in the central groove 11, and four grooves 12 are provided at the bottom of the culture box 1, and a support assembly 13 is installed in the four grooves 12;

[0039] During operation, each culture tank 2 is independently designed and can independently culture and place cells of different time periods and different types. The operation is convenient, and all independently designed mounting components 3 are integrated in a single incubator 1, eliminating the need to add multiple culture platforms for cell culture and making it convenient to carry and transfer, thereby preventing cross contamination caused by the connection of multiple culture tanks 2.

[0040] As a further solution of the present invention, a plurality of light-transmitting grooves 14 are provided on the outer wall of the incubator 1, the inner wall of the light-transmitting groove 14 is designed to be transparent, the light-transmitting groove 14 is located between two adjacent culture grooves 2, and a sealing plate 15 is installed at the opening of the light-transmitting groove 14; when the culture tube 5 in the incubator 1 is subjected to light-transmitting treatment, the sealing plate 15 at the opening of the light-transmitting groove 14 can be opened, because the inner wall of the light-transmitting groove 14 is designed to be transparent, the light-transmitting groove 14 is located between two adjacent culture grooves 2, the culture tube 5 can be observed and light-transmitted through the light-transmitting groove 14, and the sealing plate 15 can be inserted into the opening of the light-transmitting groove 14 to avoid light.

[0041] As a further solution of the present invention, a collecting chamber 16 is provided at the lower part of the interior of the incubator 1, and a solenoid valve 17 is installed at the top of the inner wall of the collecting chamber 16. The solenoid valve 17 is installed through the bottom of the inner wall of the culture tank 2, and a main discharge valve 18 is installed at the bottom of the inner wall of the collecting chamber 16. When the culture tube 5 is damaged or liquid overflows, the liquid will flow into the solenoid valve 17 below along the culture tank 2. After the solenoid valve 17 is opened, the liquid will be introduced into the collecting chamber 16, so that the collecting chamber 16 can collect all the liquids in the culture tanks 2 and can be discharged from the incubator 1 by opening the main discharge valve 18 at the bottom. Each solenoid valve 17 is independently designed to prevent cross contamination caused by the connection of multiple culture tanks 2, thereby affecting the cell culture work.

[0042] As a further solution of the present invention, the mounting assembly 3 includes a column 301, a horizontal plate 303 and a support plate 305 are fixedly connected between the column 301 and the sealing cover 4, two limiting partitions 304 are arranged between the support plate 305 and the horizontal plate 303, the limiting partitions 304 are installed on the opposite sides of the column 301 and the sealing cover 4, a plurality of clamping holes 302 are opened on the horizontal plate 303, a plurality of ball grooves 306 and leakage holes 307 are opened on the support plate 305, the bottom end of the culture tube 5 passes through the clamping hole 302 and the limiting partition 304 and is located in the ball groove 306, the column 301, the horizontal plate 303 and the support plate 305 are slidably connected in the culture trough 2; the top end of the culture tube 5 is higher than the clamping hole 302, so as to facilitate the taking and placing operation of the culture tube 5.

[0043] The culture tube 5 containing cells is placed in the mounting assembly 3, so that the bottom end of the culture tube 5 passes through the card hole 302 and the limiting partition 304 and is located in the ball groove 306. The culture tube 5 is limited by the ball groove 306 and the card hole 302, and supported by the limiting partition 304 to ensure the mounting stability of the culture tube 5; when the culture tube 5 is damaged or liquid overflows, the liquid will flow out through the leakage hole 307.

[0044] As a further solution of the present invention, the locking assembly 7 includes a slide plate 701, a slide groove 6 is respectively provided at the top and bottom of the sealing cover 4, a plurality of slots 9 are respectively provided at the top and bottom of the outer wall of the incubator 1, the slide plate 701 is slidably connected in the slide groove 6, a block 702 is fixedly connected to the slide plate 701, the block 702 is clipped in the slot 9, one side of the block 702 is designed as an arc surface and the other side is designed as a plane, and a pull rod 703 is fixed inside the slide plate 701, the end of the pull rod 703 is fixed to the handle 8, and a support spring 704 is connected to the outer sleeve of the pull rod 703, and the support spring 704 is fixed between the sealing cover 4 and the inner wall of the slide plate 701. The slide plate 701 is supported by the elastic force of the support spring 704, so that the slide plate 701 drives the block 702 to clip into the slot 9, thereby achieving the purpose of locking the sealing cover 4, improving the convenience of operation, and the sealing cover 4 can seal the opening of the culture tank 2 to prevent external dust and impurities from entering the culture tank 2.

[0045] As a further solution of the present invention, the automated culture analyzer 10 includes an automated control module 101, a fixed column 102 is installed in the middle of the automated control module 101, the fixed column 102 is installed in the central groove 11, a ferrule 103 is provided outside the automated control module 101, the ferrule 103 is connected to the top of the incubator 1, and a group of online observers 104 are respectively provided at the positions corresponding to several culture grooves 2 at the bottom of the automated control module 101, and the number of each group of online observers 104 is five, and each online observer 104 is located directly above the culture tube 5. Because each online observer 104 is located directly above the culture tube 5, the observation accuracy is guaranteed, and the cells in the culture tube 5 are observed online by the online observer 104 and the signal is transmitted to the automated control module 101, so that the culture status can be observed and analyzed in real time;

[0046] Hold the card sleeve 103 and pull it upwards, so that the card sleeve 103 drives the fixed column 102 to slide in the central groove 11 through the automatic control module 101, and the automatic control module 101 drives multiple online observers 104 to move out of the incubator 1, thereby facilitating the removal of the automated culture analyzer 10 for cleaning and maintenance.

[0047] As a further solution of the present invention, the support assembly 13 includes a fixing ring 131, on which four support rods 132 are fixed, and a universal wheel 133 is installed at the bottom end of the support rod 132. Four grooves 12 are provided at the bottom of the incubator 1, and the top end of the support rod 132 is slidably connected in the groove 12. A buffer spring 134 is connected to the outer cover of the support rod 132, and the buffer spring 134 is fixed between the bottom of the incubator 1 and the fixing ring 131. A damping spring 135 is fixed at the top end of the support rod 132, and the damping spring 135 is installed in the groove 12. The support rod 132 squeezes the buffer spring 134 through the fixing ring 131. After being compressed, the buffer spring 134 provides a reverse force to the fixing ring 131, which can play a role in shock absorbing the incubator 1, and cooperates with the damping spring 135 in the groove 12 to support the end of the support rod 132, so as to prevent the incubator 1 from vibrating too much and affecting the stability of the culture tube 5.

[0048] A method for using a fully automatic cell culture device, the method comprising the following steps:

[0049] When the cells are fully automatically cultured, the culture tube 5 containing the cells is placed in the mounting assembly 3, so that the bottom end of the culture tube 5 passes through the clamp hole 302 and the limiting partition 304 and is located in the ball groove 306, the culture tube 5 is limited by the ball groove 306 and the clamp hole 302, and the limiting partition 304 is used to support it, so as to ensure the stability of the mounting of the culture tube 5, and the top of the culture tube 5 is higher than the clamp hole 302. After the placement of the culture tube 5 is completed, the sealing cover 4 is pushed, so that the sealing cover 4 drives the culture tube 5 to move through the cross plate 303 and the supporting plate 305, so that the culture tube 5 can enter the culture tank 2 in the incubator 1;

[0050] During the movement of the sealing cover 4, the locking assembly 7 will be driven to approach the incubator 1. When the block 702 in the locking assembly 7 contacts the side wall of the incubator 1, because the side of the block 702 close to the incubator 1 is designed as an arc surface, the block 702 will drive the slide plate 701 to slide into the slide groove 6 after being compressed. When the sealing cover 4 is close to the side wall of the incubator 1, the slide plate 701 is supported by the elastic force of the support spring 704, so that the slide plate 701 drives the block 702 to be stuck in the slot 9, thereby achieving the purpose of locking the sealing cover 4, so that the sealing cover 4 can seal the opening of the culture tank 2 to prevent external dust and impurities from entering the culture tank 2. Each culture tank 2 is installed with a mounting assembly 3. The mounting assembly 3 at other positions of the incubator 1 is operated in the same way, so as to facilitate the delivery of the culture tube 5 into the incubator 1. Moreover, each culture tank 2 is independently designed, and can independently culture and take and place cells of different time periods and different types. All independently designed mounting assemblies 3 are integrated in a single incubator 1 to culture the cells in the culture tube 5 automatically.

[0051] When the cells in the culture tube 5 are cultured by the incubator 1, the bottom end of the online observer 104 in the automated culture analyzer 10 is connected to the culture tank 2, and each online observer 104 is located directly above the culture tube 5. The cells in the culture tube 5 are observed online by the online observer 104 and the signal is transmitted to the automated control module 101. The automated culture analyzer 10 can analyze the real-time data. When the culture tube 5 in the incubator 1 needs to be light-transmitted, the sealing plate 15 at the opening of the light-transmitting tank 14 can be opened. Since the inner wall of the light-transmitting tank 14 is designed to be transparent, the light-transmitting tank 14 is located between two adjacent culture tanks 2, and the culture tube 5 can be observed and light-transmitted through the light-transmitting tank 14. The sealing plate 15 can be inserted into the opening of the light-transmitting tank 14 to avoid light.

[0052] When the culture tube 5 is damaged or overflows with liquid, the liquid will flow along the culture tank 2 and into the electromagnetic valve 17 below through the leak hole 307. After the electromagnetic valve 17 is opened, the liquid will be introduced into the collection chamber 16, so that the collection chamber 16 can collect the liquid in all the culture tanks 2 and discharge it from the culture box 1 by opening the main discharge valve 18 at the bottom. Each electromagnetic valve 17 is independently designed to prevent cross contamination caused by the connection of multiple culture tanks 2, thereby affecting the cell culture work.

[0053] When the culture device is transferred, the incubator 1 is pushed to make the universal wheel 133 in the support assembly 13 roll on the ground. During the movement of the incubator 1, the universal wheel 133 will squeeze the support rod 132, and the support rod 132 will squeeze the buffer spring 134 through the fixing ring 131. After being compressed, the buffer spring 134 will provide a reverse force to the fixing ring 131, which can play a role in shock absorption of the incubator 1. The damping spring 135 in the groove 12 supports the end of the support rod 132 to prevent the incubator 1 from vibrating too much and affecting the stability of the culture tube 5.

[0054] When taking out the culture tube 5, by squeezing the two handles 8, the two handles 8 respectively pull the slide plate 701 through the pull rod 703, so that the slide plate 701 drives the card block 702 to disengage from the card slot 9, that is, the locking state between the sealing cover 4 and the incubator 1 is released, and the culture tube 5 can be taken out of the culture tank 2 by pulling the handles 8 outward, and the entire mounting assembly 3 and the culture tube 5 can be completely moved out of the culture tank 2, thereby facilitating the taking and placing of the culture tube 5. When removing the automated culture analyzer 10, hold the card sleeve 103 and pull it upward, so that the card sleeve 103 drives the fixed column 102 to slide in the central groove 11 through the automated control module 101, and the automated control module 101 drives multiple online observers 104 to move out of the incubator 1, and the automated culture analyzer 10 can be removed for daily cleaning and maintenance of the device.

Claims

1. A fully automatic cell culture device, comprising an incubator (1), characterized in that: The incubator (1) is provided with a plurality of culture troughs (2) outside, a mounting assembly (3) is installed inside the culture troughs (2), a sealing cover (4) is fixedly connected to the side of the mounting assembly (3) away from the incubator (1), the sealing cover (4) seals the opening of the culture trough (2), a plurality of culture test tubes (5) are installed in the mounting assembly (3), a sliding groove (6) is respectively provided at the top and bottom of the sealing cover (4), a locking assembly (7) is slidably connected inside the sliding groove (6), and a handle (8) is respectively fixed to the ends of the two locking assemblies (7) close to each other, and the incubator (1) is provided with a plurality of culture test tubes (5) inside. A plurality of slots (9) are respectively provided on the upper and lower sides of the outer wall, and the ends of the locking components (7) are locked in the slots (9). An automated culture analyzer (10) is installed on the top of the incubator (1), and the ends of the automated culture analyzer (10) penetrate and communicate with a plurality of culture slots (2). A central slot (11) is provided in the middle of the incubator (1), and the bottom of the automated culture analyzer (10) is installed in the central slot (11). Four grooves (12) are provided on the bottom of the incubator (1), and support components (13) are installed in the four grooves (12).

2. A fully automatic cell culture device according to claim 1, characterized in that: The outer wall of the culture box (1) is provided with a plurality of light-transmitting grooves (14), the inner wall of the light-transmitting grooves (14) is designed to be transparent, the light-transmitting grooves (14) are located between two adjacent culture tanks (2), and a sealing plate (15) is installed at the opening of the light-transmitting grooves (14).

3. A fully automatic cell culture device according to claim 1, characterized in that: A collecting bin (16) is provided at the lower part of the interior of the culture box (1), a solenoid valve (17) is installed at the top of the inner wall of the collecting bin (16), the solenoid valve (17) is installed through the bottom of the inner wall of the culture tank (2), and a main discharge valve (18) is installed at the bottom of the inner wall of the collecting bin (16).

4. The fully automatic cell culture device according to claim 1, characterized in that: The mounting assembly (3) comprises a column (301), a horizontal plate (303) and a support plate (305) are fixedly connected between the column (301) and the sealing cover (4), two limiting partitions (304) are arranged between the support plate (305) and the horizontal plate (303), and the limiting partitions (304) are installed on the opposite sides of the column (301) and the sealing cover (4), the horizontal plate (303) is provided with a plurality of clamping holes (302), the support plate (305) is provided with a plurality of ball grooves (306) and leakage holes (307), the bottom end of the culture tube (5) passes through the clamping holes (302) and the limiting partitions (304) and is located in the ball groove (306), and the column (301), the horizontal plate (303) and the support plate (305) are slidably connected in the culture tank (2).

5. The fully automatic cell culture device according to claim 1, characterized in that: The locking assembly (7) includes a slide plate (701), the slide plate (701) is slidably connected in the slide groove (6), a clamping block (702) is fixedly connected to the slide plate (701), the clamping block (702) is clamped in the clamping groove (9), one side of the clamping block (702) is designed as an arc surface and the other side is designed as a plane, and a pull rod (703) is fixed inside the slide plate (701), the end of the pull rod (703) is fixed to the handle (8), and a support spring (704) is connected to the outer shell of the pull rod (703), and the support spring (704) is fixed between the sealing cover (4) and the inner wall of the slide plate (701).

6. The fully automatic cell culture device according to claim 1, characterized in that: The automated culture analyzer (10) comprises an automated control module (101), a fixed column (102) is installed in the middle of the automated control module (101), the fixed column (102) is installed in the central groove (11), a ferrule (103) is provided on the outside of the automated control module (101), the ferrule (103) is connected to the top of the incubator (1), and a group of online observers (104) are respectively provided at the positions corresponding to a plurality of culture grooves (2) at the bottom of the automated control module (101), the number of each group of online observers (104) is five, and each online observer (104) is located directly above the culture tube (5).

7. The fully automatic cell culture device according to claim 1, characterized in that: The support assembly (13) comprises a fixing ring (131), four support rods (132) are fixed on the fixing ring (131), a universal wheel (133) is installed at the bottom end of the support rod (132), the top end of the support rod (132) is slidably connected in the groove (12), a buffer spring (134) is connected to the outer shell of the support rod (132), the buffer spring (134) is fixed between the bottom of the incubator (1) and the fixing ring (131), and a damping spring (135) is fixed at the top end of the support rod (132), and the damping spring (135) is installed in the groove (12).

8. A method for using a fully automatic cell culture device, according to any one of claims 1 to 7, characterized in that: The method of use comprises the following steps: When cells are fully automatically cultured, a culture tube (5) containing cells is placed in the mounting assembly (3) so that the bottom end of the culture tube (5) passes through the clamp hole (302) and the limiting partition (304) and is located in the ball groove (306). The culture tube (5) is limited by the ball groove (306) and the clamp hole (302) and supported by the limiting partition (304) to ensure the stability of the mounting of the culture tube (5). The top of the culture tube (5) is higher than the clamp hole (302). After the placement of the culture tube (5) is completed, the sealing cover (4) is pushed so that the sealing cover (4) drives the culture tube (5) to move through the horizontal plate (303) and the supporting plate (305), so that the culture tube (5) can enter the culture groove (2) in the incubator (1); During the movement of the sealing cover (4), the locking assembly (7) is driven to approach the incubator (1). When the block (702) in the locking assembly (7) contacts the side wall of the incubator (1), since the side of the block (702) close to the incubator (1) is designed as an arc surface, the block (702) is pressed to drive the slide plate (701) to slide into the slide groove (6). When the sealing cover (4) is tightly attached to the side wall of the incubator (1), the slide plate (701) is supported by the elastic force of the support spring (704), so that the slide plate (701) drives the block (702) to be stuck into the slot (9), thereby achieving the locking of the sealing cover (4). The sealing cover (4) is used to seal the opening of the culture tank (2) to prevent dust and impurities from entering the culture tank (2). Each culture tank (2) is installed with a mounting assembly (3). The mounting assembly (3) at other positions of the culture box (1) is operated in the same way, so as to facilitate the delivery of the culture tube (5) into the culture box (1). Moreover, each culture tank (2) is independently designed and can independently culture and take out cells of different time periods and different types. All independently designed mounting assemblies (3) are integrated in a single culture box (1) to fully automatically culture the cells in the culture tube (5). When the cells in the culture tube (5) are cultured by the incubator (1), the bottom end of the online observer (104) in the automated culture analyzer (10) is connected to the culture tank (2), and each online observer (104) is located directly above the culture tube (5). The cells in the culture tube (5) are observed online by the online observer (104) and the signal is transmitted to the automated control module (101). The automated culture analyzer (10) can analyze the real-time data. When the culture tube (5) in the incubator (1) needs to be light-transmitted, the sealing plate (15) at the opening of the light-transmitting tank (14) can be opened. Since the inner wall of the light-transmitting tank (14) is designed to be transparent, the light-transmitting tank (14) is located between two adjacent culture tanks (2). The culture tube (5) can be observed and light-transmitted through the light-transmitting tank (14). The sealing plate (15) can be inserted into the opening of the light-transmitting tank (14) to avoid light. When the culture tube (5) is damaged or overflows with liquid, the liquid will flow along the culture tank (2) and through the leak hole (307) into the electromagnetic valve (17) below. After the electromagnetic valve (17) is opened, the liquid will be introduced into the collection chamber (16), so that the collection chamber (16) can collect the liquid in all the culture tanks (2) and discharge it from the culture box (1) by opening the main discharge valve (18) at the bottom. Each electromagnetic valve (17) is independently designed to prevent cross contamination caused by the connection of multiple culture tanks (2) and thus affect the cell culture work; When the culture device is transferred, the culture box (1) is pushed to make the universal wheel (133) in the support assembly (13) roll on the ground. During the movement of the culture box (1), the universal wheel (133) will squeeze the support rod (132). The support rod (132) squeezes the buffer spring (134) through the fixing ring (131). After being compressed, the buffer spring (134) will provide a reverse force to the fixing ring (131), which can play a role in shock absorption of the culture box (1). The damping spring (135) in the groove (12) is used to support the end of the support rod (132), so as to prevent the culture box (1) from vibrating too much and affecting the stability of the culture tube (5). When taking out the culture tube (5), the two handles (8) are squeezed so that the two handles (8) respectively pull the slide plate (701) through the pull rod (703), so that the slide plate (701) drives the block (702) to disengage from the slot (9), that is, the locking state between the sealing cover (4) and the incubator (1) is released, and the culture tube (5) can be taken out of the culture tank (2) by pulling the handles (8) outwards, and the entire mounting assembly (3) and the culture tube (5) can be completely moved out of the culture tank (2), and the culture tube (5) can be removed from the culture tank (2). In order to facilitate the taking and placing of the culture tube (5), when removing the automated culture analyzer (10), the card sleeve (103) is held and pulled upward, so that the card sleeve (103) drives the fixed column (102) to slide in the central groove (11) through the automated control module (101), and the automated control module (101) drives the multiple online observers (104) to move out of the incubator (1), and the automated culture analyzer (10) can be removed to facilitate daily cleaning and maintenance of the device.

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