Water purification activated carbon microorganism pre-evaluation method

Abstract

The invention relates to the technical field of water treatment, in particular to a pre-evaluation method for microorganisms of water purification activated carbon, according to the method, activated carbon is subjected to oscillation elution treatment by adopting an eluent prepared from sterilized municipal water and Tween 80, and the content of the microorganisms after elution has no significant difference from the content of the microorganisms in effluent after the activated carbon is on line. The online activated carbon use condition can be directly simulated, whether the activated carbon can be used for water purification or not is pre-evaluated, the situation that a water purification system is polluted after follow-up unqualified activated carbon is online is avoided, Tween 80 in eluent can protect microbial cells, the microbial cells are protected against damage in the oscillation process, and the service life of the activated carbon is prolonged. Compared with the prior art that only sterile municipal water is used, the Tween 80 has the advantages that the number of microorganisms subjected to oscillation elution is closer to the number of microorganisms 6 hours after new carbon is put on line, and the test result is closer to the true value after activated carbon is put on line.

Description

Technical Field

[0001] The invention relates to the technical field of water treatment, and in particular to a water purification activated carbon microorganism pre-evaluation method. Background Art

[0002] In water treatment technology, wood activated carbon is usually used as an adsorbent to treat municipal tap water. The new carbon that is usually purchased may be contaminated with microorganisms due to storage, sealing and transportation conditions. If the new carbon contaminated with microorganisms is directly loaded into the water purification device, the problem of excessive microorganisms in the effluent water will occur. Currently, the evaluation of the new carbon microorganisms can only be obtained from the effluent water quality of the water purification device. However, after using activated carbon contaminated with microorganisms, in order to prevent the subsequent water purification process from being further contaminated, the machine needs to be shut down for thorough cleaning and disinfection, which increases cleaning costs and reduces production efficiency. Therefore, it is necessary to provide a method for pre-evaluation of microorganisms in water purification wood activated carbon.

[0003] Since activated carbon is a porous material, microorganisms are adsorbed inside the pores of activated carbon and are difficult to test. Currently, the test of microorganisms in activated carbon is mainly carried out through the activated carbon biomass test method, which mainly includes colorimetry and dehydrogenase activity method. The colorimetric method uses an extraction solution to extract the phospholipids in the cells, and then uses the colorimetric method to measure the content of phospholipids contained in the phospholipids to evaluate the size of the biomass. The dehydrogenase activity method uses TTC to be reduced to TC with a stable color by dehydrogenase, and then the dehydrogenase activity is determined by the colorimetric method to judge the content of microorganisms in the activated carbon; the above methods for the content of activated carbon microorganisms all use chemical methods to indirectly evaluate the total biomass in the activated carbon, and the activated carbon used in water treatment does not need to be completely sterilized when used, as long as the microbial content in the effluent of the activated carbon after it is put into use meets the standard. Therefore, it is only necessary to provide a method that can simulate the use of online activated carbon, but a large amount of water is required when using a small test device to test the activated carbon. Therefore, it is of great significance to provide a method that can simulate the use of online activated carbon without a large amount of water and complex equipment. Summary of the invention

[0004] In order to overcome the problem that there is a big difference between the activated carbon biomass testing method in the prior art and the online activated carbon usage, the present invention provides a water purification activated carbon microbial pre-evaluation method, which uses an eluent made of sterilized municipal water and Tween 80 to perform oscillation elution treatment on the activated carbon. There is no significant difference between the microbial content after elution and the microbial content in the effluent after the activated carbon is put into use. The online activated carbon usage can be directly simulated to pre-evaluate whether the activated carbon can be used for water purification, thereby avoiding the subsequent unqualified activated carbon from contaminating the water purification system after it is put into use. In addition, the method does not require the use of a large amount of water, is simple to operate and has high resource utilization efficiency.

[0005] The specific technical scheme of the present invention is: A water purification activated carbon microorganism pre-evaluation method comprises the following steps: sterile municipal water and Tween 80 are used to prepare an eluent, the eluent and an activated carbon sample are mixed and then oscillated to prepare a test sample, and after the test sample is allowed to stand for stratification, the supernatant of the test sample is taken to perform a total colony count test.

[0006] The present invention provides a method for pre-evaluating microorganisms of activated carbon for water purification. The method adopts an eluent made of sterile municipal water and Tween 80 to oscillate and elute the activated carbon to prepare a test sample. When testing the microbial content in the test sample, the microbial content measured by the method has no significant difference with the sample 6 hours after the new carbon is put into operation. The above method can simulate whether the microbial content of the effluent 6 hours after the activated carbon is put into operation meets the standard, can pre-evaluate the contamination of the activated carbon before the activated carbon is put into operation, can avoid the activated carbon from contaminating the subsequent process after it is put into operation, ensure the quality of the effluent, avoid downtime maintenance caused by the pollution of the processing raw materials, and improve the production efficiency. The eluent in the present invention is a mixture of sterile municipal water and Tween 80. Tween 80 can protect the microbial cells and protect them from being destroyed during the oscillation process, thereby avoiding the problem of inaccurate data caused by the destruction of the microorganisms in the subsequent test. Compared with using only sterile municipal water, the number of microorganisms after oscillation and elution of Tween 80 in the present invention is closer to the number of microorganisms 6 hours after the new carbon is put into operation, and the test result is closer to the real value after the activated carbon is put into operation.

[0007] Preferably, the mass ratio of sterile municipal water to Tween 80 in the eluent is 0.98-0.99:0.01-0.02.

[0008] Preferably, the oscillating elution includes a first-stage oscillating elution and a second-stage oscillating elution with vortex oscillation.

[0009] Preferably, the conditions for the oscillation elution include: a soaking time of 12 to 18 hours and an oscillation time of 5 to 360 minutes.

[0010] Preferably, the one-stage oscillation elution comprises the following steps: firstly, static soaking is performed, and after the static soaking is completed, the sample is placed on a vortex oscillation device for oscillation treatment to prepare a test sample.

[0011] Preferably, the conditions for the one-stage oscillation elution include: a soaking time of 12 hours and an oscillation time of 5 to 360 minutes.

[0012] Preferably, the two-stage oscillation elution comprises the following steps: firstly, soaking once, then oscillating once, then soaking twice, then oscillating twice to prepare a test sample.

[0013] Preferably, the conditions for the two-stage oscillation elution include: a soaking time of 18 hours, an oscillation time of 6 hours, and an oscillation speed of 240 rpm.

[0014] Preferably, the total colony count test uses a colony counter.

[0015] Preferably, the mass of the activated carbon sample is 5 to 100 g.

[0016] The oscillation elution in the present invention respectively adopts a one-stage oscillation elution and a two-stage oscillation elution method. The one-stage oscillation elution method adopts a vortex oscillation device for oscillation elution, and the two-stage elution adopts a shaking table for oscillation elution. The one-stage oscillation elution can make the mixture of the eluent and the activated carbon sample produce a vortex through the vortex oscillation device, which can accelerate the detachment of the eluent from the microorganisms in the micropores of the activated carbon and can perform rapid elution. When rapid testing is required, the one-stage oscillation elution method can be used for rapid evaluation. The two-stage oscillation elution method uses a shaking table oscillation elution. The shaking table oscillation elution method is more gentle and can avoid the damage of oscillation to the microorganisms. When time is sufficient, the use of a shaking table oscillation can more realistically restore the content of microorganisms in the water effluent 6 hours after the new carbon is online.

[0017] Compared with the prior art, this application has the following technical effects: (1) The present invention uses an eluent made of sterilized municipal water and Tween 80 to perform an oscillation elution treatment on the activated carbon. The microbial content after elution is not significantly different from the microbial content of the effluent water after the activated carbon is put into operation. The use of activated carbon in the online environment can be directly simulated to pre-evaluate whether the activated carbon can be used for water purification, thereby avoiding the contamination of the water purification system by unqualified activated carbon after it is put into operation. In addition, the method does not require the use of a large amount of water, is simple to operate, and has high resource utilization efficiency. (2) The eluent in the present invention is a mixture of sterile municipal water and Tween 80. Tween 80 can protect microbial cells from being destroyed during the oscillation process, thereby avoiding the problem of inaccurate data caused by the destruction of microorganisms in subsequent tests. Compared with using only sterile municipal water, the number of microorganisms after oscillation elution with Tween 80 in the present invention is closer to the number of microorganisms 6 hours after the new carbon is put into operation, and the test result is closer to the actual value after the activated carbon is put into operation. DETAILED DESCRIPTION

[0018] The present invention will be further described below in conjunction with the embodiments.

[0019] Embodiment 1: A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 20CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 1% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0020] Embodiment 2: A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 180CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 1% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0021] Embodiment 3: A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent water 6 hours after the new carbon is put into operation is 600CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 1% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0022] Embodiment 4: A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 1821CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 1% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0023] Embodiment 5: A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 5g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 20CFU / ml), add it to a sterilized 15ml test tube, then add 10ml of sterile municipal water containing 1% Tween 80, seal the test tube and place it on a vortex oscillator for a period of oscillation elution at room temperature to obtain an eluate, which includes standing and soaking at room temperature for 12h, and then oscillating on a vortex oscillator for 5min. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0024] Embodiment 6: A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 5g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 180CFU / ml), add it to a sterilized 15ml test tube, then add 10ml of sterile municipal water containing 1% Tween 80, seal the test tube and place it on a vortex oscillator for a period of oscillation elution at room temperature to obtain an eluate, which includes standing and soaking at room temperature for 12h, and then oscillating on a vortex oscillator for 5min. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0025] Embodiment 7: A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 5g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 600CFU / ml), add it to a sterilized 15ml test tube, then add 10ml of sterile municipal water containing 2% Tween 80, seal the test tube and place it on a vortex oscillator for a period of oscillation elution at room temperature to obtain an eluate, which includes standing and soaking at room temperature for 12h, and then oscillating on a vortex oscillator for 5min. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0026] Embodiment 8: A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 5g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 1821CFU / ml), add it to a sterilized 15ml test tube, then add 10ml of sterile municipal water containing 2% Tween 80, seal the test tube and place it on a vortex oscillator for a period of oscillation elution at room temperature to obtain an eluate. One period of oscillation elution includes standing and soaking at room temperature for 12h, and then oscillating on a vortex oscillator for 5min. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0027] Comparative Example 1: Compared with Example 1, the eluent in Comparative Example 1 was only sterile municipal water; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 20CFU / ml), add it to a sterilized 500ml conical flask, then add 140g of sterile municipal water, seal the conical flask and place it on a shaker for two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0028] Comparative Example 2: Compared with Example 2, the eluent in Comparative Example 2 was only sterile municipal water; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is online is 180CFU / ml), add it to a sterilized 500ml conical flask, then add 140g of sterile municipal water, seal the conical flask and place it on a shaker for two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is online.

[0029] Comparative Example 3: Compared with Example 3, the eluent in Comparative Example 3 was only sterile municipal water; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is online is 600CFU / ml), add it to a sterilized 500ml conical flask, then add 140g of sterile municipal water, seal the conical flask and place it on a shaker for two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is online.

[0030] Comparative Example 4: Compared with Example 4, the eluent in Comparative Example 4 was only sterile municipal water; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is online is 1821CFU / ml), add it to a sterilized 500ml conical flask, then add 140g of sterile municipal water, seal the conical flask and place it on a shaker for two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is online.

[0031] Comparative Example 5: Compared with Example 1, the eluent in Comparative Example 5 is sterile municipal water containing 4% Tween 80; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 20CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 4% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0032] Comparative Example 6: Compared with Example 2, the eluent in Comparative Example 6 is sterile municipal water containing 4% Tween 80; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 180CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 4% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0033] Comparative Example 7: Compared with Example 3, the eluent in Comparative Example 7 is sterile municipal water containing 4% Tween 80; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent water 6 hours after the new carbon is put into operation is 600CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 4% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0034] Comparative Example 8: Compared with Example 4, the eluent in Comparative Example 8 is sterile municipal water containing 4% Tween 80; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent water 6 hours after the new carbon is put into operation is 1821CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 4% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0035] Comparative Example 9: Compared with Example 1, the eluent in Comparative Example 9 is sterile municipal water containing 0.6% Tween 80; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 20CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 0.6% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0036] Comparative Example 10: Compared with Example 2, the eluent in Comparative Example 10 is sterile municipal water containing 0.6% Tween 80; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent water 6 hours after the new carbon is put into operation is 180CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 0.6% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0037] Comparative Example 11: Compared with Example 3, the eluent in Comparative Example 11 is sterile municipal water containing 0.6% Tween 80; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent water 6 hours after the new carbon is put into operation is 600CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 0.6% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0038] Comparative Example 12: Compared with Example 4, the eluent in Comparative Example 12 is sterile municipal water containing 0.6% Tween 80; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent water 6 hours after the new carbon is put into operation is 1821CFU / ml), add it to a sterilized 500ml conical flask, and then add 140g of sterile municipal water containing 0.6% Tween 80. After sealing the conical flask, place the conical flask on a shaker and perform two-stage oscillation elution at room temperature to obtain an eluate. The frequency of the two-stage oscillation elution is two static oscillation treatments, the static soaking time is 18h, the shaking time is 6h, and the shaking speed is 240rpm. After the eluate is allowed to stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate. The measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0039] Comparative Example 13: Compared with Example 1, in Comparative Example 13, the samples were allowed to stand and soak for 48 hours; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 20CFU / ml), add it to a sterilized 500ml conical flask, then add 140g of sterile municipal water containing 1% Tween 80, seal the conical flask and let it stand at room temperature for 48h to obtain an eluent, let the eluent stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluent, the measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0040] Comparative Example 14: Compared with Example 2, in Comparative Example 14, the static soaking was performed for 48 hours; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is online is 180CFU / ml), add it to a sterilized 500ml conical flask, then add 140g of sterile municipal water containing 1% Tween 80, seal the conical flask and let it stand at room temperature for 48h to obtain an eluent, let the eluent stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluent, the measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is online.

[0041] Comparative Example 15: Compared with Example 3, in Comparative Example 15, the static soaking was performed for 48 hours; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is online is 600CFU / ml), add it to a sterilized 500ml conical flask, then add 140g of sterile municipal water containing 1% Tween 80, seal the conical flask and let it stand at room temperature for 48h to obtain an eluate, let the eluate stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluate, the measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is online.

[0042] Comparative Example 16: Compared with Example 4, in Comparative Example 16, the static soaking was performed for 48 hours; A water purification activated carbon microorganism pre-evaluation method comprises the following steps: Weigh 100g of activated carbon sample (coconut shell activated carbon, the microbial content of the effluent 6 hours after the new carbon is put into operation is 1821CFU / ml), add it to a sterilized 500ml conical flask, then add 140g of sterile municipal water containing 1% Tween 80, seal the conical flask and let it stand at room temperature for 48h to obtain an eluent, let the eluent stand until stratification, take the supernatant and use a colony counter to count the colonies of the supernatant of the eluent, the measured number of colonies is the number of microorganisms 6 hours after the simulated activated carbon is put into operation.

[0043] Test example: The number of microorganisms measured in the above Examples 1 to 8 and Comparative Examples 1 to 16 was statistically analyzed; The analysis results are shown in Table 1; Table 1 Test results As shown in Table 1, a two-stage testing method was adopted in Examples 1 to 4, and the results obtained in Examples 1 to 4 were not significantly different from the microbial content in the effluent 6 hours after the new carbon was put into operation. Examples 5 to 8 adopted a one-stage oscillation elution method to quickly test the number of microorganisms in the activated carbon, and the results obtained in Examples 5 to 8 were not significantly different from the microbial content in the effluent 6 hours after the new carbon was put into operation. The above results indicate that the method provided by the present invention can truly simulate the content of microorganisms in the effluent 6 hours after the activated carbon is put into operation, and can evaluate whether the activated carbon is contaminated by microorganisms when the activated carbon is online.

[0044] Compared with Examples 1 to 4, Comparative Examples 1 to 4 only used sterile municipal water as eluent. The results showed that when only sterile municipal water was used, if the microbial content in the activated carbon was low, there was no significant difference between the microbial content obtained by eluting with sterile municipal water alone and the microbial content after the activated carbon was put online. However, when the microbial content in the activated carbon was high, the microbial content obtained by eluting with sterile municipal water alone was significantly lower than the microbial content in the effluent 6 hours after the activated carbon was put online. The above results show that only using sterile municipal water as eluent cannot effectively elute the microorganisms in the activated carbon micropores, resulting in significant differences between the test results and the actual results of the microbial content in the effluent 6 hours after the activated carbon was put online, and cannot truly reflect the effluent situation after the activated carbon was put online.

[0045] Compared with Examples 1 to 4, in Comparative Examples 5 to 8, an excess of Tween 80 was added to the eluent. The results showed that the addition of excess Tween 80 did not significantly improve the elution of microorganisms. In addition, when the content of microorganisms in the activated carbon was high, the addition of excess Tween 80 would cause the test result to be greater than the content of microorganisms in the effluent 6 hours after the activated carbon was put on line, resulting in inaccurate test results. Compared with Examples 1 to 4, Comparative Examples 9 to 12 have a lower content of Tween 80 added to them. The results show that if the amount of Tween 80 is too small, the test structure will be significantly lower than the microbial content in the effluent 6 hours after the activated carbon is online. From the results of Comparative Examples 5 to 12 and Examples 1 to 4, it can be seen that the eluent with the addition of Tween 80 can elute the microorganisms in the activated carbon more effectively, making the test results closer to the actual results of the microorganisms in the effluent 6 hours after the activated carbon is online. In addition, it was found through testing that if the content of Tween 80 is too small, the protective effect on the microorganisms in the activated carbon is small, and some microorganisms will be destroyed, resulting in a significant reduction in the test data.

[0046] Compared with Examples 1 to 4, Comparative Examples 13 to 16 did not use oscillation. The results showed that the test results without oscillation were significantly lower than the microbial content in the effluent 6 hours after the activated carbon was online. The above results indicate that oscillation has a significant promoting effect on the elution of microorganisms in the activated carbon, and can better enable the microorganisms to detach from the microporous structure of the activated carbon.

[0047] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any way. Any simple modification, change and equivalent transformation made to the above embodiment based on the technical essence of the present invention still falls within the protection scope of the technical solution of the present invention.

Claims

1. A water purification activated carbon microorganism pre-evaluation method, characterized in that: The following steps are involved: Sterile municipal water and Tween 80 were used to make eluent, and the eluent and activated carbon sample were mixed and then eluted by oscillation to prepare a test sample. After the test sample was allowed to stand and stratify, the supernatant of the test sample was taken for a total colony count test.

2. The water purification activated carbon microorganism pre-evaluation method according to claim 1, characterized in that: The mass ratio of sterile municipal water to Tween 80 in the eluent is 0.98-0.99:0.01-0.

02.

3. The water purification activated carbon microorganism pre-evaluation method according to claim 2, characterized in that: The oscillating elution includes a first-stage oscillating elution and a second-stage oscillating elution vortex oscillation.

4. The water purification activated carbon microorganism pre-evaluation method according to claim 1 or 3, characterized in that: The conditions for the oscillation elution include: a soaking time of 12 to 18 hours and an oscillation time of 5 to 360 minutes.

5. The water purification activated carbon microorganism pre-evaluation method according to claim 3, characterized in that: The one-stage oscillation elution comprises the following steps: firstly, standing and soaking, and then placing the sample on a vortex oscillation device for oscillation treatment to prepare a test sample.

6. The water purification activated carbon microorganism pre-evaluation method according to claim 3 or 5, characterized in that: The conditions of the one-stage oscillation elution include: a soaking time of 12 hours and an oscillation time of 5 minutes.

7. The water purification activated carbon microorganism pre-evaluation method according to claim 3, characterized in that: The two-stage oscillation elution comprises the following steps: firstly, soaking once, then oscillating once, then soaking twice, then oscillating twice to prepare a test sample.

8. The water purification activated carbon microorganism pre-evaluation method according to claim 3 or 7, characterized in that: The conditions of the two-stage oscillation elution include: a soaking time of 18 hours, an oscillation time of 6 hours, and an oscillation speed of 240 rpm.

9. The water purification activated carbon microorganism pre-evaluation method according to claim 1, characterized in that: The total colony count test uses a colony counter.

10. The water purification activated carbon microorganism pre-evaluation method according to claim 1, characterized in that: The mass of the activated carbon sample is 5-100g.

Images