Multi-joint detection dry biochemical detection chip (kit) and use method thereof

Abstract

The invention discloses a multi-joint detection dry type biochemical detection chip (kit) and a use method, and belongs to the field of biochemical detection.The detection chip comprises a handle assembly and a chip assembly connected with the handle assembly; according to the application, creative design is performed on serum obtained by blood filter pressing and a micro-channel for the serum, smooth, rapid and efficient filter pressing of the blood is ensured, the blood smoothly flows into a sequence point position in the micro-channel, particularly, the filter element adopts a two-stage structure, rough filtration is performed firstly, then fine filtration is performed, the filtering effect is improved, and the service life of the filter element and the micro-channel is prolonged. Most blood cells are filtered out in a centrifugal-free manner, serum is obtained, the precision of subsequent detection is improved, and meanwhile, smooth filter pressing and efficiency improvement are facilitated; in addition, the viscosity of serum, the length of the micro-channel, the flow, the driving pressure and exhaust are considered in the micro-channel; the micro-flow of the serum is smoothly carried out, the full reaction of the filled sequence point location and the drying reagent is realized, and the subsequent optical detection and the detection accuracy are facilitated.

Description

Technical Field

[0001] The present application relates to the field of biochemical detection technology, and more specifically, to a multiplex dry biochemical detection chip (test kit) and a method of use. Background Art

[0002] The existing biochemical detection devices in the prior art have the following problems: large size, large amount of blood required for detection, long detection time, high detection cost, and limited location and environment for use of biochemical detection devices;

[0003] Currently, the demand for miniaturization and convenience in blood biochemical testing is becoming more and more prominent. The difficulty in miniaturization and convenience of blood biochemical testing lies in the difficulty of integrated innovation of testing methods and testing equipment. Summary of the invention

[0004] The present application provides a multi-test dry biochemical detection chip (kit) and a method of use, which can solve the current increasingly prominent demand for miniaturization and convenience of blood biochemical detection. The difficulty of miniaturization and convenience of blood biochemical detection lies in the technical problem of the difficulty of integrated innovation of detection methods and detection equipment;

[0005] On one hand, the present application discloses a multi-test dry biochemical detection chip (kit), comprising a detection chip, wherein the detection chip has a filter press cavity, a sequence point, and a microchannel connecting the filter press cavity to the sequence point; the filter press cavity forms the injection port on the upper surface of the detection chip, and a trace amount of blood drips into the filter press cavity of the detection chip through the injection port;

[0006] The filter press cavity has a filter press element in the detection chip for filtering blood cells to obtain serum; the sequence points include a plurality of detection points for optical penetration inspection; the detection points form detection point cavities in the detection chip; the detection point cavities are connected to the filter press cavity through microchannels in a one-to-one correspondence; the sequence points are configured with corresponding desiccated reagents in the corresponding detection point cavities;

[0007] The trace amount of blood is pushed by the driving air pressure to filter and micro-flow to the sequence points in the detection chip, and at the same time, the air in the microchannel and the detection point cavity in the detection chip is gradually squeezed out until the serum obtained by the filter fills all the detection point cavities of the sequence points;

[0008] The plane position where the detection chip corresponds to the microchannel and the detection point cavity is water-blocking and air-permeable;

[0009] The air in the microchannel and the detection point cavity in the detection chip is discharged along the plane direction of the detection chip, and the serum liquid is confined in the microchannel and the detection point cavity;

[0010] The detection chip has at least 7 types, and the 7 types correspond one by one to 7 corresponding detection types. The 7 detection types are liver function detection, kidney function detection, virus infection detection, glycolipid detection, pancreas detection, kidney glycolipid detection and myocardial detection. Each detection type corresponds to multiple detection items, and each detection item corresponds to at least one detection point and one dried reagent.

[0011] Preferably, the detection chip is provided with an identity module and a positioning module cooperating with the detection host, the identity module corresponds to the ID information of the detection chip, the ID information of the detection chip includes the type of the detection chip and the corresponding drying reagent information, the drying reagent information includes the type of drying reagent and the calibration curve.

[0012] Preferably, the detection chip includes a handle assembly and a chip assembly connected to the handle assembly;

[0013] The filter press chamber is arranged on the handle assembly, the filter press chamber forms an inlet on the upper side of the handle assembly, and the filter press chamber forms a filter outlet on the lower side of the handle assembly;

[0014] The sequence points and microfluidic channels are arranged on the chip assembly;

[0015] The chip assembly has a fixed end and a free end along the length direction;

[0016] The chip assembly is connected to the handle assembly at a position close to the fixed end;

[0017] The sequence point is arranged at a position of the chip component close to the free end.

[0018] Preferably, the microfluidic channel includes at least one mainstream segment and multiple branch segments; the branch segments are arranged in a one-to-one correspondence with the detection point cavity, the detection point cavity is connected to the mainstream segment through the branch segments in a one-to-one correspondence, the mainstream segment is connected to the filter outlet of the filter pressure cavity, and the other end of the mainstream segment in the microfluidic channel is provided with a loading inlet, which extends to the outside of the chip assembly and abuts against the outlet of the filter pressure cavity.

[0019] Preferably, the filter press element comprises a coarse filter element and a fine filter element which are arranged in sequence, the average pore size of the coarse filter element is larger than the average pore size of the fine filter element; and the filtration path of the coarse filter element is much larger than that of the fine filter element.

[0020] Preferably, when the detection chip is working, the driving air pressure is set to P0 and the filter pressure difference is , Microfluidic drive pressure difference ;

[0021] ;

[0022] The serum after filtration enters the microchannel directly, and the filtration flow Microchannel flow 2 are equal, set to calculate flow ;

[0023] Micro-blood filtration:

[0024] To calculate the flow rate, A is the cross-sectional area of ​​the filter element. is the permeability of the filter element, is the viscosity of blood, is the filter pressure difference; is the height of the filter element;

[0025] When filtering serum microfluidics:

[0026] When the cross section of the microchannel is circular; the diameter of the main flow section of the microchannel is

[0027] in, The diameter of the main flow section of the microchannel, 1 is the viscosity of serum; is the length of the mainstream segment; To calculate the flow rate, The pressure difference is the driving force of the microfluidic flow;

[0028] in, The diameter of the main flow section of the microchannel, 1 is the viscosity of serum; is the length of the tributary section; To calculate the flow rate, Microfluidic drive pressure difference;

[0029] When the cross section of the microfluidic channel is rectangular;

[0030] The cross section of the main flow section of the microchannel satisfies:

[0031]

[0032] in, To calculate the flow rate, is the width of the rectangular section of the main flow section, is the height of the rectangular section of the main flow section; ≥ ; is the width of the rectangular section of the tributary section, is the height of the rectangular section of the tributary section; ≥ ; 1 is the viscosity of serum; is the length of the mainstream segment; is the length of the tributary section.

[0033] Preferably, the filter press chamber comprises a pressure chamber and a filter chamber arranged in sequence from top to bottom;

[0034] The horizontal cavity diameter of the pressure cavity gradually decreases from top to bottom, and a filter hole with a small diameter is formed at the bottom of the pressure cavity; the filter hole is located in the handle assembly near the chip assembly; the filter hole extends to the outside of the handle assembly to form a filter cavity; the filter element is arranged in the filter cavity;

[0035] The inner wall of the pressure chamber forms a pressure-inflow field pointing to the filter chamber; the surface of the pressure-inflow field is hydrophobic, and the pressure-inflow field includes a first flow field and a second flow field arranged in sequence from top to bottom; the first flow field is a circular arc groove, and the second flow field is a conical groove; the second flow field is located on a side of the first flow field close to the chip assembly; the streamline slope of the second flow field is greater than the streamline slope of the first flow field;

[0036] The upper side of the handle assembly has a pressure-bearing surface; the pressure cavity opening of the pressure cavity is located in the middle of the pressure-bearing surface, the pressure-bearing surface forms a stepped tubular interface corresponding to the pressure cavity opening, and an arc-shaped transition is formed between the outer side of the tubular interface and the pressure-bearing surface.

[0037] Preferably, the chip assembly has at least a three-layer structure, and the three-layer structure includes upper and lower sealing layers and a microfluidic carrier layer in the middle;

[0038] The microfluidic carrier layer is breathable along its plane direction; a microfluidic channel is opened on the upper side of the microfluidic carrier layer corresponding to the microfluidic channel; the upper and lower surface sealing layers are adhered to and sealed to form the microfluidic channel by sealing the upper and lower sides of the microfluidic carrier layer in the middle.

[0039] Preferably, it also includes an accessory for packaging the detection chip;

[0040] The accessories include a sealing bag, a sealing sticker and / or a packaging box; the sealing sticker seals the sample inlet and the detection point of the filter press cavity on the detection chip;

[0041] The test chip is built into a sealed bag and packed in a packaging box.

[0042] Another aspect of the present application discloses a method for using a multi-test dry biochemical detection chip (kit), characterized in that it comprises the steps of: obtaining a detection chip, and dripping a trace amount of blood from an injection port of the detection chip;

[0043] Step 2: The detection chip cooperates with the detection host, and the detection host recognizes the detection chip. After completion, air pressure is generated at the inlet position of the detection chip to push the trace amount of blood dripped into the pressure filter cavity to be filtered out from the outlet and enter the microchannel, and then enter the detection point cavity corresponding to multiple detection points through the microchannel, so as to fully react with the dried reagent;

[0044] Step 3: The detection host performs optical penetration inspection on multiple detection points on the detection chip to obtain optical joint inspection information, and analyzes and calculates the detection results based on the optical joint inspection information.

[0045] The beneficial effects of the technical solution provided by this application are:

[0046] The present application creatively designs a detection chip for blood biochemical detection, and realizes a multi-test dry biochemical detection without centrifugal filtration. The present application designs a handle assembly and a filter press chamber on the handle assembly, and generates air pressure at the inlet of the filter press chamber to push a trace amount of blood dripped on the detection chip to pass through the filter press chamber for filtration. The filtered serum enters the microchannel of the chip assembly connected to the handle assembly, and enters the sequence points through the microchannel respectively to react with the drying reagent built into the sequence points. The present application creatively designs the microchannel for obtaining serum by blood filtration and the microchannel for serum to ensure smooth, rapid and efficient filtration of blood. And it flows smoothly into the sequence points in the microfluidic channel, especially the design of the filter element and the microfluidic channel, wherein the filter element adopts a two-level structure, first coarse filtration and then fine filtration, which improves the filtration effect and realizes centrifugal filtration to remove most of the blood cells, obtains relatively pure serum, improves the accuracy of subsequent detection, and is conducive to the smooth progress of pressure filtration and improves efficiency; in addition, the structural design of the microfluidic channel takes into account the viscosity of the serum, the length of the microfluidic channel, the flow rate, the driving pressure and the exhaust; so that the microflow of the serum proceeds smoothly, filling the sequence points and fully reacting with the drying reagent, which is conducive to the subsequent optical detection and detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments of the present application. Obviously, the drawings described below are only some embodiments of the present application, and those skilled in the art can obtain other drawings based on these drawings without creative work.

[0048] Figure 1 A schematic diagram of a detection chip disclosed in an exemplary embodiment of the present application;

[0049] Figure 2 A schematic diagram of the cooperation between the detection chip and the detection host disclosed in the exemplary embodiment of the present application;

[0050] Figure 3A schematic diagram of the structure decomposition of the detection chip disclosed in the exemplary embodiment of the present application;

[0051] Figure 4 It is a schematic diagram of the structural decomposition of the handle assembly in the detection chip disclosed in the exemplary embodiment of the present application;

[0052] Figure 5 This is a schematic diagram of the internal structure of a handle assembly in a detection chip disclosed in an exemplary embodiment of the present application;

[0053] Figure 6 It is a schematic plan view of a handle assembly in a detection chip disclosed in an exemplary embodiment of the present application;

[0054] Figure 7 A schematic structural diagram of a filter press element disclosed in an exemplary embodiment of the present application;

[0055] Figure 8 A schematic plan view of a chip assembly in a detection chip disclosed in an exemplary embodiment of the present application;

[0056] Fig. 9 A schematic plan view of a microfluidic channel in a detection chip disclosed in an exemplary embodiment of the present application;

[0057] Fig.10 A schematic cross-sectional view of a microfluidic channel in a detection chip disclosed in an exemplary embodiment of the present application;

[0058] Fig.11 An exemplary physical front perspective view of a detection chip disclosed in an exemplary embodiment of the present application;

[0059] Fig.12 An exemplary real object reverse stereoscopic view of a detection chip disclosed in an exemplary embodiment of the present application;

[0060] Fig.13 An exemplary physical exploded view of a detection chip disclosed in an exemplary embodiment of the present application;

[0061] Fig.14 An exemplary physical side view of a detection chip disclosed in an exemplary embodiment of the present application;

[0062] Fig.15 An exemplary physical side exploded view of a detection chip disclosed in an exemplary embodiment of the present application. DETAILED DESCRIPTION

[0063] The embodiments of the present application are described in detail below, and examples of the embodiments are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present application, and cannot be interpreted as limiting the present application.

[0064] It will be understood by those skilled in the art that, unless expressly stated, the singular forms "one", "said", and "the" used herein may also include plural forms. It should be further understood that the term "comprising" used in the specification of the present disclosure refers to the presence of the features, integers, steps, operations, elements, and / or components, but does not exclude the presence or addition of one or more other features, integers, steps, operations, elements, components, and / or groups thereof. It should be understood that when we refer to an element as being "connected" or "coupled" to another element, it may be directly connected or coupled to the other element, or there may be intermediate elements. In addition, the "connection" or "coupling" used herein may include wireless connection or wireless coupling. The term "and / or" used herein includes all or any unit and all combinations of one or more associated listed items.

[0065] In order to make the purpose, technical solution and advantages of the present application clearer, the implementation methods of the present application will be further described in detail below with reference to the accompanying drawings:

[0066] Please refer to 1 to Fig.15 , the exemplary embodiment of the present application provides a multi-test dry biochemical detection chip (kit), including a detection chip 200, wherein the detection chip 200 cooperates with a detection host 100;

[0067] The detection chip 200 has a filter press cavity 202, a sequence point 203, and a microchannel 204 connecting the filter press cavity 202 to the sequence point 203; the filter press cavity 202 forms the injection port 201 on the upper surface of the detection chip 200, and a trace amount of blood 300 drips into the filter press cavity 202 of the detection chip 200 through the injection port 201;

[0068] The filter press cavity 202 has a filter press element 400 in the detection chip 200 for filtering blood cells to obtain serum 301; the sequence point 203 includes a plurality of detection points B for optical penetration inspection; the detection point B forms a detection point cavity 205 in the detection chip 200; the detection point cavity 205 is connected to the filter press cavity 202 through a microchannel 204 in a one-to-one correspondence; the sequence point 203 is configured with a corresponding dry reagent 206 in the corresponding detection point cavity 205;

[0069] The trace amount of blood 300 is pushed by the driving air pressure 1000 generated by the detection host 100 to filter and flow to the sequence point 203 in the detection chip 200, and at the same time, the air 2000 in the microchannel 204 and the detection point cavity 205 in the detection chip 200 is gradually squeezed out until the serum 301 obtained by the filter fills all the detection point cavities 205 of the sequence point 203;

[0070] The plane position of the detection chip 200 corresponding to the microchannel 204 and the detection point cavity 205 is water-blocking and air-permeable;

[0071] The air 2000 in the microchannel 204 and the detection point cavity 205 in the detection chip 200 is discharged along the plane direction of the detection chip 200, and the serum 301 liquid is confined in the microchannel 204 and the detection point cavity 205;

[0072] In an exemplary embodiment, the detection chip 200 has at least 7 types, and the 7 types correspond one by one to 7 corresponding detection types. The 7 detection types are liver function detection, kidney function detection, viral infection detection, glycolipid detection, pancreatic detection, kidney glycolipid detection and myocardial detection. Each detection type corresponds to multiple detection items, and each detection item corresponds to at least one detection point B and one dried reagent 206.

[0073] In an exemplary embodiment, the detection chip 200 is provided with an identity module 20 and a positioning module DW cooperating with the detection host 100, the identity module 20 corresponds to the ID information of the detection chip 200, the ID information of the detection chip 200 includes the type of the detection chip 200 and the corresponding dried reagent information, the dried reagent information includes the type of dried reagent 206 and the calibration curve, the calibration curve.

[0074] In an exemplary embodiment, the detection chip 200 includes a handle assembly 21 and a chip assembly 22 connected to the handle assembly 21;

[0075] In an exemplary embodiment, the handle assembly 21 is a strip-shaped structure, and the handle assembly 21 has a hand-held end 211 and a working end 212 along the length direction;

[0076] A hand-held portion 2111 is provided on one side of the handle assembly 21 close to the hand-held end 211; a hand-held anti-slip area 2112 is provided on the upper side of the hand-held portion 2111, and the hand-held anti-slip area 2112 is circular and has anti-slip patterns therein;

[0077] The lower side of the handheld part 2111 is provided with an identity module 20; the detection host 300 detects the identity module 20 to obtain the detection target information of the detection chip 200, so as to facilitate the subsequent matching detection; in an exemplary embodiment, the identity module 20 is a QR code;

[0078] In an exemplary embodiment, the filter press chamber 202 is disposed on the handle assembly 21, the filter press chamber 202 forms an inlet 201 on the upper side of the handle assembly 21, and the filter press chamber 202 forms a filter outlet 2020 on the lower side of the handle assembly 21;

[0079] In an exemplary embodiment, the upper side of the handle assembly 21 has a pressure-bearing surface 210; the injection port 201 is located on the pressure-bearing surface 210, and the pressure-bearing surface 210 forms a stepped tubular interface 2011 corresponding to the injection port 201, and an arc-shaped transition is formed between the outer side of the tubular interface 2011 and the pressure-bearing surface 210.

[0080] In an exemplary embodiment, the elastic pressure bowl 600 is docked with the tubular interface 2011, and the elastic pressure bowl is elastically deformed by the detection host 100 to wrap and squeeze the sample inlet 201 of the filter cavity, thereby generating a driving gas pressure 1000;

[0081] In an exemplary embodiment, the filter press chamber 202 includes a pressure chamber 2021 and a filter chamber 2022 arranged sequentially from top to bottom;

[0082] The horizontal cavity diameter r0 of the pressure cavity 2021 gradually decreases from top to bottom, and a small-diameter filter hole GLK is formed at the bottom of the pressure cavity 2021; the filter hole GLK is located in the handle assembly 21 near the chip assembly 22; the filter hole GLK extends to the outside of the handle assembly 21 to form a filter cavity GLQ; the filter element 400 is arranged in the filter cavity GLQ;

[0083] The inner wall of the pressure chamber 2021 forms a pressure-inflow field YLC pointing to the filter chamber GLQ; the surface of the pressure-inflow field YLC is hydrophobic, and the pressure-inflow field YLC includes a first flow field LC1 and a second flow field LC2 arranged in sequence from top to bottom; the first flow field LC1 is a circular arc groove, and the second flow field LC2 is a conical groove; the second flow field LC2 is located on a side of the first flow field LC1 close to the chip assembly 22; the streamline slope of the second flow field LC2 is greater than the streamline slope of the first flow field;

[0084] The pressure flow field YLC has a scraping flange 2022; the scraping flange 2022 is arranged at the connection between the first flow field LC1 and the second flow field LC2, and the scraping flange 2022 is in an arc shell shape, and the arc opening faces the filter cavity GLQ, so as to facilitate scraping of trace blood 300;

[0085] In an exemplary embodiment, the pressure-inflow field YLC is an inner conical surface; the pressure-inflow field YLC may be an inner spherical surface;

[0086] The filter cavity GLQ forms a filter outlet 2020 at the bottom of the handle assembly 21;

[0087] In an exemplary embodiment, the filter hole GLK is circular or elliptical; the central axis of the filter cavity GLQ vertically passes through the handle assembly 21;

[0088] In an exemplary embodiment, the central axis of the filter chamber GLQ can obliquely penetrate the handle assembly 21, and the central axis of the filter chamber GLQ has an angle with the vertical direction; in an exemplary embodiment, the angle is 30 degrees;

[0089] The aperture of the filter cavity GLQ is equal from top to bottom; in some embodiments, the aperture of the filter cavity GLQ may gradually decrease from top to bottom, which is conducive to centralized filtration and improves the filtration effect;

[0090] In an exemplary embodiment, the filter press element 400 includes a coarse filter element 401 and a fine filter element 402 which are arranged in sequence, and the average pore size of the coarse filter element 401 is larger than the average pore size of the fine filter element 402; the coarse filtration path 4011 of the coarse filter element 401 is much larger than the fine filtration path 4021 of the fine filter element 402.

[0091] In an exemplary embodiment, the coarse filter element 401 is a columnar structure that matches the filter cavity GLQ. The coarse filter element 401 is built into the filter cavity GLQ and has an interference fit with the filter cavity GLQ.

[0092] In an exemplary embodiment, a limiting flange is provided in the filter cavity GLQ for clamping and fixing the coarse filter element 401; in some embodiments, the limiting flange may be arranged on the inner wall of the filter cavity GLQ in a spiral shape;

[0093] In an exemplary embodiment, the fine filter element 402 is in the shape of a cake, one end of the fine filter element 402 is embedded in the filter cavity GLQ and connected to the coarse filter element 401; the other end of the fine filter element 402 is fixed to a side of the handle assembly 21 corresponding to the filter outlet 2020 through a filter element fixing portion 403;

[0094] In an exemplary embodiment, the filter element fixing portion 403 is a fixing patch, and the fine filter element 402 is attached to the filter outlet 2020;

[0095] In an exemplary embodiment, the pore size of the coarse filter element 401 obeys the normal distribution N(d1,σ12), d1 is the average pore size of the coarse filter element 401, σ1 is the standard deviation of the pore size of the coarse filter element 401; d1 is 7um-10um; σ1 is 1um-2um;

[0096] The porosity of the coarse filter element 401 is 0.5-0.7;

[0097] The pore size of the fine filter element 402 obeys the normal distribution N(d2,σ22), μ2 is the average pore size of the fine filter element 402, σ2 is the standard deviation of the pore size of the fine filter element 402; d2 is 5um-7um;

[0098] σ2 is 0.5um-1um; the porosity of the fine filter element 402 is 0.3-0.5;

[0099] The sequence points 203 and the microchannels 204 are arranged on the chip assembly 22;

[0100] The chip assembly 22 has a fixed end 221 and a free end 222 along the length direction;

[0101] The chip assembly 22 is connected to the handle assembly 21 at a position close to the fixed end 221;

[0102] The sequence point 203 is disposed at a position of the chip assembly 22 close to the free end 222 .

[0103] The sequence point 203 is composed of a plurality of detection points B; detection point cavities 205 are provided in the chip assembly 22 corresponding to the detection points B one by one;

[0104] There is no restriction on the arrangement of the detection points;

[0105] In an exemplary embodiment, the detection points B are arranged in a straight line;

[0106] In an exemplary embodiment, the detection points B are arranged in a straight line and evenly spaced;

[0107] In an exemplary embodiment, the detection points B are arranged in a straight line with non-uniform intervals;

[0108] In an exemplary embodiment, the detection points B are arranged in an arc-shaped curve;

[0109] In an exemplary embodiment, the detection points B are evenly spaced and arranged in an arc-shaped curve;

[0110] In an exemplary embodiment, the detection points B are arranged in an arc-shaped curve with uneven intervals;

[0111] The chip assembly 22 is connected to the handle assembly 21 at a position close to the fixed end 221;

[0112] In an exemplary embodiment, the positioning module DW includes a positioning and limiting protrusion DW1, which is a triangular protrusion to facilitate positioning and limiting.

[0113] In an exemplary embodiment, the positioning module DW includes a positioning groove DW2, which is a semicircular groove and is arranged on the free end 222 of the chip assembly 22. The detection host is provided with a positioning protrusion that cooperates with the positioning groove corresponding to the detection chip, and the positioning protrusion is a cylinder; there are two positioning grooves and positioning protrusions, and they are asymmetrically designed to prompt the detection chip to be plugged in with the injection port facing upward.

[0114] In an exemplary embodiment, a step TJK is provided at the bottom of the working end 212 of the handle assembly 21; the filter outlet 2020 of the filter pressure chamber 202 is located on the step TJK; the step TJK facilitates the positioning and installation of the chip assembly 22; the filter element fixing portion 403 and the chip assembly 22 are sequentially attached to the step TJK; the microchannel 204 is provided with a loading port ZRK on one side of the chip assembly, and the loading port ZRK is connected to the filter outlet 2020; a filter element through hole 4031 is provided on the fixing patch, and the filter element through hole 4031 connects the filter outlet 2020 and the loading port ZRK; the fine filter element 402 is located between the loading port ZRK and the filter outlet 2020;

[0115] In an exemplary embodiment, the chip assembly 22 has at least a three-layer structure, and the three-layer structure includes upper and lower sealing layers MC and a middle microfluidic carrier layer ZC;

[0116] The microfluidic carrier layer ZC is breathable along its plane direction; a microfluidic channel is opened on the upper side of the microfluidic carrier layer ZC corresponding to the microfluidic channel 204; two surface sealing layers MC are attached to and seal the upper and lower sides of the microfluidic carrier layer ZC in the middle to form the microfluidic channel 204.

[0117] The microchannel 204 includes at least one main flow segment 2041 and a plurality of branch flow segments 2042; the branch flow segments 2042 are arranged in one-to-one correspondence with the detection point cavities 205, and the detection point cavities 205 are connected to one end of the main flow segment 2041 through the branch flow segments 2042 in one-to-one correspondence; the loading port ZRK is connected to the other end of the main flow segment 2041;

[0118] In an exemplary embodiment, the detection host 100 is provided with a first detection positioning point B0 at a serial point 203 corresponding to the detection chip 200, and the detection light in the first detection positioning point B0 can pass through the chip component without obstruction; the serial point 203 includes M detection points B from B1 to BM; in an exemplary embodiment, M is 9.

[0119] The first detection positioning point B0 is set outside the detection chip 200 or on the detection chip 200;

[0120] In an exemplary embodiment, when the detection chip is working, the driving gas pressure is set to , Filter pressure difference , Microfluidic drive pressure difference ;

[0121] ;

[0122] The serum after filtration enters the microchannel directly, and the filtration flow Microchannel flow Equal, set to calculate flow ;

[0123] Micro-blood filtration:

[0124] To calculate the flow rate, is the cross-sectional area of ​​the filter element, is the permeability of the filter element, is the viscosity of blood, is the filter pressure difference; is the height of the filter element;

[0125] When filtering serum microfluidics:

[0126] When the cross section of the microchannel is circular; the diameter of the main flow section of the microchannel is

[0127] in, The diameter of the main flow section of the microchannel, is the viscosity of serum; is the length of the mainstream segment; To calculate the flow rate, The pressure difference is the driving force of the microfluidic flow;

[0128] in, The diameter of the main flow section of the microchannel, is the viscosity of serum; is the length of the tributary section; To calculate the flow rate, Microfluidic drive pressure difference;

[0129] When the cross section of the microfluidic channel is rectangular;

[0130] The cross section of the main flow section of the microchannel satisfies:

[0131]

[0132] in, To calculate the flow rate, is the width of the rectangular section of the main flow section, is the height of the rectangular section of the main flow section; ≥ ; is the width of the rectangular section of the tributary section, is the height of the rectangular section of the tributary section; ≥ ; is the viscosity of serum; is the length of the mainstream segment; is the length of the tributary section.

[0133] The exemplary embodiment of the present application discloses that it also includes an accessory for packaging the detection chip 200;

[0134] The accessories include a sealing bag, a sealing sticker and / or a packaging box; the sealing sticker seals the injection port 201 and the detection point B on the detection chip 200;

[0135] The detection chip 200 is placed in a sealed bag and packed in a packaging box.

[0136] The exemplary embodiment of the present application discloses a method for using a multi-test dry biochemical detection chip (kit), characterized in that it comprises the steps of: obtaining a detection chip, and dripping a trace amount of blood from an injection port of the detection chip;

[0137] Step 2: The detection chip cooperates with the detection host, and the detection host recognizes the detection chip. After completion, air pressure is generated at the inlet position of the detection chip to push the trace amount of blood dripped into the pressure filter cavity to be filtered out from the outlet and enter the microchannel, and then enter the detection point cavity corresponding to multiple detection points through the microchannel, so as to fully react with the dried reagent;

[0138] Step 3: The detection host performs optical penetration inspection on multiple detection points on the detection chip to obtain optical joint inspection information, and analyzes and calculates the detection results based on the optical joint inspection information.

Claims

1. A multi-detection dry biochemical detection chip (kit), characterized in that: The detection chip comprises a filter press cavity, a sequence of points, and a microchannel connecting the filter press cavity to the sequence of points; the filter press cavity forms the injection port on the upper surface of the detection chip, and a trace amount of blood drips into the filter press cavity of the detection chip through the injection port; The filter press cavity has a filter press filter element in the detection chip for filtering blood cells to obtain serum; the sequence points include multiple detection points for optical penetration inspection; The detection point forms a detection point cavity in the detection chip; The detection point cavities are connected to the filter press cavities through microchannels in a one-to-one correspondence; the sequence points are equipped with corresponding drying reagents in the corresponding detection point cavities; The trace amount of blood is pushed by the driving air pressure to filter and microflow to the sequence points in the detection chip, and at the same time, the air in the microchannel and detection point cavity in the detection chip is gradually squeezed out until the serum obtained by filter filling all the detection point cavities of the sequence points.

2. The multiple-detection dry biochemical detection chip (kit) according to claim 1, characterized in that: The plane position where the detection chip corresponds to the microchannel and the detection point cavity is water-blocking and air-permeable; The air in the microchannel and the detection point cavity in the detection chip is discharged along the plane direction of the detection chip, and the serum liquid is confined in the microchannel and the detection point cavity; The detection chip has at least 7 types, and the 7 types correspond to 7 corresponding detection types, namely liver function detection, kidney function detection, virus infection detection, glycolipid detection, pancreas detection, kidney glycolipid detection and myocardial detection. Each detection type corresponds to multiple detection items, and each detection item corresponds to at least one detection point and one drying reagent; The detection chip is provided with an identity module and a positioning module cooperating with the detection host. The identity module corresponds to the ID information of the detection chip. The ID information of the detection chip includes the type of the detection chip and the corresponding drying reagent information. The drying reagent information includes the type of drying reagent.

3. The multiple-detection dry biochemical detection chip (kit) according to claim 2, characterized in that: The detection chip includes a handle component and a chip component connected to the handle component; The filter press chamber is arranged on the handle assembly, the filter press chamber forms an inlet on the upper side of the handle assembly, and the filter press chamber forms a filter outlet on the lower side of the handle assembly; The sequence points and microfluidic channels are arranged on the chip assembly; The chip assembly has a fixed end and a free end along the length direction; The chip assembly is connected to the handle assembly at a position close to the fixed end; The sequence point is arranged at a position of the chip component close to the free end.

4. The multiple-test dry biochemical detection chip (kit) according to claim 3, characterized in that: The microfluidic channel includes at least one mainstream section and multiple branch sections; the branch sections are arranged in a one-to-one correspondence with the detection point cavity, the detection point cavity is connected to the mainstream section through the branch sections in a one-to-one correspondence, the mainstream section is connected to the filter outlet of the filter pressure cavity, and the other end of the mainstream section in the microfluidic channel is provided with a loading inlet, which extends to the outside of the chip component and abuts against the connected filter outlet.

5. The multiple-detection dry biochemical detection chip (kit) according to claim 3, characterized in that: The filter press element comprises a coarse filter element and a fine filter element which are arranged in sequence, wherein the average pore size of the coarse filter element is larger than the average pore size of the fine filter element; and the filtering path of the coarse filter element is much larger than that of the fine filter element.

6. The multiple-test dry biochemical detection chip (kit) according to claim 3, characterized in that: When the detection chip is working, the driving air pressure is set to P0, and the filter pressure difference is set to , Microfluidic drive pressure difference ; ； The serum after filtration enters the microchannel directly, and the filtration flow Microchannel flow 2 are equal, set to calculate flow ; Micro-blood filtration: ； To calculate the flow rate, A is the cross-sectional area of ​​the filter element. is the permeability of the filter element, is the viscosity of blood, is the filter pressure difference; is the height of the filter element; When filtering serum microfluidics: When the cross section of the microchannel is circular; the diameter of the main flow section of the microchannel is in, The diameter of the main flow section of the microchannel, 1 is the viscosity of serum; is the length of the mainstream segment; To calculate the flow rate, The pressure difference is the driving force of the microfluidic flow; in, The diameter of the main flow section of the microchannel, 1 is the viscosity of serum; is the length of the tributary section; To calculate the flow rate, The pressure difference is the driving force of the microfluidic flow; When the cross section of the microfluidic channel is rectangular; The cross section of the main flow section of the microchannel satisfies: in, To calculate the flow rate, is the width of the rectangular section of the mainstream section, is the height of the rectangular section of the main flow section; ≥ ; is the width of the rectangular section of the tributary section, is the height of the rectangular section of the tributary section; ≥ ; 1 is the viscosity of serum; is the length of the mainstream segment; is the length of the tributary section.

7. The multiple-test dry biochemical detection chip (kit) according to claim 3, characterized in that: The filter press chamber comprises a pressure chamber and a filter chamber arranged in sequence from top to bottom; The horizontal cavity diameter of the pressure cavity gradually decreases from top to bottom, and a filter hole with a small diameter is formed at the bottom of the pressure cavity; the filter hole is located in the handle assembly near the chip assembly; the filter hole extends to the outside of the handle assembly to form a filter cavity; the filter element is arranged in the filter cavity; The inner wall of the pressure chamber forms a pressure-inflow field pointing to the filter chamber; the surface of the pressure-inflow field is hydrophobic, and the pressure-inflow field includes a first flow field and a second flow field arranged in sequence from top to bottom; the first flow field is a circular arc groove, and the second flow field is a conical groove; the second flow field is located on a side of the first flow field close to the chip assembly; the streamline slope of the second flow field is greater than the streamline slope of the first flow field; The upper side of the handle assembly has a pressure-bearing surface; the pressure cavity opening of the pressure cavity is located in the middle of the pressure-bearing surface, the pressure-bearing surface forms a stepped tubular interface corresponding to the pressure cavity opening, and an arc-shaped transition is formed between the outer side of the tubular interface and the pressure-bearing surface.

8. The multiple-test dry biochemical detection chip (kit) according to claim 3, characterized in that: The chip assembly has at least a three-layer structure, and the three-layer structure includes upper and lower sealing layers and a microfluidic carrier layer in the middle; The microfluidic carrier layer is breathable along its plane direction; a microfluidic channel is opened on the upper side of the microfluidic carrier layer corresponding to the microfluidic channel; the upper and lower surface sealing layers are adhered to and sealed to form the microfluidic channel by sealing the upper and lower sides of the microfluidic carrier layer in the middle.

9. The multiple-test dry biochemical detection chip (kit) according to any one of claims 1 to 8, characterized in that: Also included are accessories for packaging the detection chip; The accessories include a sealing bag, a sealing sticker and / or a packaging box; the sealing sticker seals the sample inlet and the detection point of the filter press cavity on the detection chip; The test chip is built into a sealed bag and packed in a packaging box.

10. A method for using the multiple-detection dry biochemical detection chip (kit) according to any one of claims 1 to 8, characterized in that: The method comprises the steps of: obtaining a detection chip, and dripping a trace amount of blood from an injection port of the detection chip; Step 2: The detection chip cooperates with the detection host, and the detection host recognizes the detection chip. After completion, air pressure is generated at the inlet position of the detection chip to push the trace amount of blood dripped into the pressure filter cavity to be filtered out from the outlet and enter the microchannel, and then enter the detection point cavity corresponding to multiple detection points through the microchannel, so as to fully react with the dried reagent; Step 3: The detection host performs optical penetration inspection on multiple detection points on the detection chip to obtain optical joint inspection information, and analyzes and calculates the detection results based on the optical joint inspection information.

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