Phospholipid detection and analysis method for infant formula milk powder

Abstract

The invention relates to the field of detection, in particular to a method for detecting and analyzing phospholipid in infant formula milk powder, which can predict the content of phospholipid in breast milk of a user so as to determine matched milk powder for the user, so that the user can feed the infant with the matched milk powder and the breast milk of the user, and a reasonable amount of phospholipid is provided for the infant. In addition, due to the fact that the phospholipid content in breast milk of the user can change in the lactation period, the method can predict the change trend of the phospholipid supply amount caused by the change in the lactation period so as to recognize the time period when the phospholipid supply amount exceeds the range, and corresponding feeding mode adjustment suggestions are given for the time period. And the phospholipid supply amount of a user to a baby in the whole lactation period is kept at a reasonable level.

Description

Technical Field

[0001] The present invention relates to the detection field, and in particular to a phospholipid detection and analysis method for infant formula milk powder. Background Art

[0002] Phospholipids play an important role in the brain development, nervous system development, and liver protection of infants and young children. Breast milk contains many types of phospholipids, which can effectively ensure the growth and development of infants and young children. When conditions permit, parents prefer to breastfeed their infants, but due to insufficient milk secretion or other limitations, many families can only feed their infants with a combination of breast milk and formula milk. The content of various phospholipids in breast milk varies depending on the mother's dietary structure. In addition, the phospholipid content of different milk powders on the market varies, and the content of various phospholipids is not specifically marked. Therefore, when purchasing milk powder, most families find it difficult to consider the complementary content of phospholipids in milk powder and breast milk secreted by the mother, which may lead to the use of a combination of breast milk and formula milk, resulting in insufficient phospholipid intake or excessive phospholipid intake (which may easily cause gastrointestinal discomfort in the baby), which is not good for the baby's health. Summary of the invention

[0003] Based on this, it is necessary to provide a phospholipid detection and analysis method for infant formula milk powder in response to the above problems, the method comprising: S1: Determine the content of various phospholipids in each type of milk powder by liquid chromatography-tandem mass spectrometry, and establish a milk powder phospholipid content database based on the determination results; S2: When receiving an analysis request from any user, inquire about the user's diet structure and breastfeeding information, wherein the breastfeeding information includes the amount of breastfeeding and the amount of milk powder used; S3: Determine the phospholipid content change curve in the user's breast milk during the lactation cycle based on historical data and dietary structure; S4: Determine the phospholipid supply curve based on the lactation amount and the phospholipid content change curve; S5: determining the matching milk powder of the user from the milk powder phospholipid content database, and determining the phospholipid supply increase amount corresponding to the matching milk powder, so as to adjust the phospholipid supply curve so that the initial section of the phospholipid supply curve is within a preset phospholipid content range; S6: identifying the out-of-range section of the phospholipid supply curve, adjusting the feeding method according to the out-of-range section, and adjusting the phospholipid supply curve accordingly, so that the phospholipid supply curve is always within the preset phospholipid content range; S7: Send the adjusted phospholipid supply curve, the corresponding adjustment time point and the adjusted feeding method to the user.

[0004] Preferably, determining the content of various phospholipids in each milk powder by liquid chromatography-tandem mass spectrometry comprises: Preparation of the assay solution: weigh 2 g of the milk powder sample in a 50 mL centrifuge tube, add 20 mL of water, and record the total mass of the milk powder sample and water; after vortex mixing, heat the sample solution in a 40°C constant temperature shaking water bath for 30 min; weigh 0.5 g of the sample solution in a 15 mL centrifuge tube, add 0.9 mL of pure water and then add 5 mL of dichloromethane-methanol solution, mix well, place the centrifuge tube on an ultrasonic cleaner for ultrasonic cleaning at 40°C for 10 min, place the centrifuge tube on a centrifuge for centrifugation at 8000 r / min for 10 min, collect the lower layer liquid, add 5 mL of dichloromethane-methanol solution again, mix well, place the centrifuge tube on an ultrasonic cleaner for ultrasonic cleaning at 40°C for 10 min, place the centrifuge tube on a centrifuge for centrifugation at 8000 r / min for 10 min, collect the lower layer liquid, combine the lower layer liquids collected twice, pass through a 0.22 μm microporous filter membrane, dissolve and fix the volume with dichloromethane-methanol to obtain the assay solution.

[0005] The test solution is measured by liquid chromatography-tandem mass spectrometry to obtain the phospholipid concentration in the test solution; The liquid chromatography-tandem mass spectrometry instrument transmits the phospholipid concentration to the computer device, and the computer device calculates the phospholipid content of the sample based on the phospholipid concentration; Preferably, the dietary structure is the food variety and corresponding food intake of the user in a set dietary cycle in the past; the historical data includes the content of each phospholipid in the colostrum of lactating women under various dietary structures, and the average change ratio of various phospholipids at each preset time point in the lactation cycle; the phospholipid content change curve in the breast milk of the user during the lactation cycle determined according to the historical data and the dietary structure includes: S31: comparing the user's dietary structure with each dietary structure in the historical data to determine the first content of each phospholipid in the user's colostrum; S32: For each phospholipid, a first coordinate system is generated, wherein the abscissa of the first coordinate system is the lactation cycle, and the ordinate is the content of the phospholipid; S33: determining the second content of the phospholipid at each preset time point in the lactation cycle according to the first content of the phospholipid and the corresponding average change ratio, and obtaining a coordinate point corresponding to each preset time point, wherein the abscissa of the coordinate point is the preset time point, and the ordinate is the corresponding second content; S34: marking the coordinate points in the first coordinate system, and connecting the coordinate points with a smooth curve to obtain a content variation curve of the phospholipid in the user's breast milk.

[0006] Preferably, comparing the user's dietary structure with each dietary structure in the historical data to determine the first content of each phospholipid in the user's colostrum includes: For each phospholipid, determine the associated elements of the phospholipid and the associated food types of the associated elements; Calculating a first intake of the associated element by the user according to the amount of the associated food variety consumed by the user; Calculate the second intake of related elements for each lactating woman in historical data; Selecting a second intake amount greater than the first intake amount and a second intake amount less than the first intake amount, and identifying the gap ratio of the first intake amount between the two second intake amounts; The difference between the contents of the phospholipid corresponding to the two second intakes is calculated, and the first content is determined according to the difference and the intervening ratio.

[0007] Preferably, colostrum is breast milk at the beginning of a lactation cycle, the preset time point corresponding to the colostrum is the starting point of the lactation cycle, and the average change ratio is the ratio of the content of a phospholipid in breast milk at the preset time point to the content of the corresponding phospholipid in colostrum; Determining the second content of the phospholipid at each preset time point during the lactation cycle according to the first content of the phospholipid and the corresponding average change ratio includes: For each phospholipid, the average change ratio of the phospholipid corresponding to each time point is retrieved; The first content is multiplied by the average change ratio of each adjustment to obtain the second content corresponding to each time point.

[0008] Preferably, determining the phospholipid supply curve according to the lactation amount and the phospholipid content change curve comprises: Corresponding to each first coordinate system, a second coordinate system is generated, wherein the abscissa of the second coordinate system is the nursing period and corresponds to the abscissa of the first coordinate system, and the ordinate is the corresponding phospholipid supply; Multiply the ordinate of each coordinate point of the phospholipid content change curve in the first coordinate system by the lactation amount to obtain the corresponding phospholipid supply, and then obtain a coordinate point in the second coordinate system; The coordinate points on each second coordinate system are connected with a smooth curve to obtain a phospholipid supply curve.

[0009] Preferably, determining the matching milk powder of the user from the milk powder phospholipid content database comprises: S51: selecting a milk powder from a milk powder phospholipid content database as an alternative milk powder; S52: for each phospholipid, multiply the content of the phospholipid in the alternative milk powder by the amount of milk powder to obtain the increased supply of the phospholipid; S53: in the second coordinate system corresponding to the phospholipid, simulating the phospholipid supply curve to increase the supply increase; S54: generating a first horizontal line and a second horizontal line on the second coordinate system, wherein the first horizontal line corresponds to a preset minimum content of the phospholipid, and the second horizontal line corresponds to a preset maximum content of the phospholipid; S55: identifying a curve point on the phospholipid supply curve that is lower than the first horizontal line for the first time or higher than the second horizontal line for the first time, and then identifying the abscissa of the curve point as the limit-exceeding time point; S56: determining the earliest time point from the over-limit time points corresponding to each phospholipid as the target time point of the candidate milk powder; S57: Repeat steps S51 to S56 until the target time point of each candidate milk powder is obtained, and determine the candidate milk powder with the latest corresponding target time point as the matching milk powder; The phospholipid supply curve is adjusted, that is, each phospholipid supply curve is improved as a whole to match the increased supply of phospholipids corresponding to the milk powder.

[0010] Preferably, identifying the out-of-range section of the phospholipid supply curve, adjusting the feeding method according to the out-of-range section, and adjusting the phospholipid supply curve accordingly includes: S61: determining the earliest time point among the over-limit time points of each phospholipid supply curve as the adjustment time point; S62: changing the dosage ratio of the breastfeeding amount and the milk powder dosage after the adjustment time point, so that the sections of the phospholipid supply curves after the adjustment time point are adjusted accordingly; S63: Determine whether the adjustment time point can be postponed by adjusting the dosage ratio. If so, determine the dosage ratio that postpones the adjustment time point for the longest time as the target ratio, adjust the section of the phospholipid supply curve after the adjustment time point according to the target ratio, and then determine a new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the nursing cycle; S64: If not, re-determine the matching milk powder for the segment after the adjustment time point, and adjust the segment after the adjustment time point accordingly to obtain a new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the feeding cycle.

[0011] Preferably, determining whether the adjustment time point can be postponed by adjusting the dosage ratio includes: S631: Determine all dosage ratios; S632: selecting a dosage ratio, for each phospholipid supply curve, adjusting the section of the phospholipid supply curve after the adjustment time point according to the dosage ratio, and identifying the over-limit time point of the section after adjustment; S633: determining the earliest time point of the over-limit time points corresponding to each phospholipid supply curve, identifying whether the earliest time point is later than the adjustment time point, and if so, determining whether the adjustment time point can be postponed by adjusting the dosage ratio; S634: If not, execute steps S632 to S634 until it is determined that the adjustment time point can be postponed by adjusting the dosage ratio, or if the earliest time point cannot be made later than the adjustment time point, it is determined that the adjustment time point cannot be postponed by adjusting the dosage ratio.

[0012] The present invention provides a phospholipid detection and analysis method for infant formula milk powder. The method comprises the following steps: determining the content of various phospholipids in each milk powder by liquid chromatography-tandem mass spectrometry, and establishing a milk powder phospholipid content database according to the determination results; when an analysis request is received from any user, inquiring the user's diet structure and breastfeeding information; determining a phospholipid content change curve in the user's breast milk during a breastfeeding cycle according to historical data and the diet structure; determining a phospholipid supply curve according to the breastfeeding amount and the phospholipid content change curve; determining the user's matching milk powder from the milk powder phospholipid content database, and determining the phospholipid supply increase corresponding to the matching milk powder, so as to adjust the phospholipid supply curve so that the initial section of the phospholipid supply curve is within a preset phospholipid content range; identifying an out-of-range section of the phospholipid supply curve, and adjusting the feeding method according to the out-of-range section, so as to adjust the feeding method according to the out-of-range section. The phospholipid supply curve is adjusted accordingly so that the phospholipid supply curve is always within the preset phospholipid content range, and the adjusted phospholipid supply curve, the corresponding adjustment time point and the adjusted feeding method are sent to the user; in the present application, the phospholipid content in the user's breast milk can be predicted, so as to determine the matching milk powder for the user, so that the user can use the matching milk powder and her own breast milk for feeding, provide a reasonable amount of phospholipids for the baby, and ensure the healthy growth of the baby. Moreover, since the phospholipid content in the user's breast milk will change during the lactation cycle, the present application can predict the changing trend of the phospholipid supply caused by the change during the lactation cycle, so as to identify the time period when the phospholipid supply exceeds the range, and give corresponding feeding method adjustment suggestions for the time period, so that the phospholipid supply given to the baby by the user during the entire lactation cycle is kept at a reasonable level. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 A flowchart of a method for detecting and analyzing phospholipids in infant formula milk powder provided in one embodiment; Figure 2 This is a diagram of the implementation environment of the phospholipid detection and analysis method for infant formula milk powder provided in one embodiment; Figure 3 The present invention is a schematic diagram of adjusting the phospholipid supply curve of the phospholipid detection and analysis method of infant formula milk powder provided in one embodiment. DETAILED DESCRIPTION

[0014] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0015] It is understood that the terms "first", "second", etc. used in the present invention may be used herein to describe various elements, but unless otherwise specified, these elements are not limited by these terms. These terms are only used to distinguish a first element from another element. For example, a first xx script may be referred to as a second xx script, and similarly, a second xx script may be referred to as a first xx script without departing from the scope of the present invention.

[0016] like Figure 1 As shown, in one embodiment, a method for detecting and analyzing phospholipids in infant formula milk powder is provided, the method comprising: S1: Determine the content of various phospholipids in each type of milk powder by liquid chromatography-tandem mass spectrometry, and establish a milk powder phospholipid content database based on the determination results; S2: When receiving an analysis request from any user, inquire about the user's diet structure and breastfeeding information, wherein the breastfeeding information includes the amount of breastfeeding and the amount of milk powder used; S3: Determine the phospholipid content change curve in the user's breast milk during the lactation cycle based on historical data and dietary structure; S4: Determine the phospholipid supply curve based on the lactation amount and the phospholipid content change curve; S5: determining the matching milk powder of the user from the milk powder phospholipid content database, and determining the phospholipid supply increase amount corresponding to the matching milk powder, so as to adjust the phospholipid supply curve so that the initial section of the phospholipid supply curve is within a preset phospholipid content range; S6: identifying the out-of-range section of the phospholipid supply curve, adjusting the feeding method according to the out-of-range section, and adjusting the phospholipid supply curve accordingly, so that the phospholipid supply curve is always within the preset phospholipid content range; S7: Send the adjusted phospholipid supply curve, the corresponding adjustment time point and the adjusted feeding method to the user.

[0017] In this embodiment, if Figure 2As shown, the 'determination of the content of various phospholipids in each milk powder by liquid chromatography-tandem mass spectrometry' in step S1 is performed by the staff through the cooperation of liquid chromatography-tandem mass spectrometry and computer equipment, and the 'establishment of a milk powder phospholipid content database according to the determination results' in step S1 is performed by the computer equipment; steps S2 to S7 are performed by the computer equipment; the computer equipment can be an independent physical server or terminal, or a server cluster composed of multiple physical servers, and can be a cloud server that provides basic cloud computing services such as cloud servers, cloud databases, cloud storage and CDN; the user communicates with the computer equipment through the user terminal (such as a mobile phone, a laptop computer, a tablet computer and other terminals), so that the computer equipment can receive the user's analysis request and exchange information with the user terminal.

[0018] In this embodiment, each type of milk powder can be all milk powders sold on the market; milk powder often contains a variety of phospholipids, such as phosphatidylcholine, phosphatidylinositol, sphingomyelin, etc.; the analysis request is a request sent by the user for the computer device to determine the required milk powder and how to match the breastfeeding amount and milk powder dosage; when the computer device receives the analysis request, it first sends authorization information to the user terminal, and the authorization information includes the execution steps of the method and the user data that needs to be called, so that the user can understand its process. After the user sends back the consent information through the user terminal, the method can be executed; In this embodiment, the dietary structure refers to the food varieties and corresponding food intake of the user in a set dietary cycle in the past (such as one month or other duration); different dietary structures will provide different component elements for synthetic breast milk, thereby resulting in different contents of various phospholipids in breast milk; in this embodiment, the historical data is data that can reflect the relationship between the dietary structure and the phospholipid content in breast milk, therefore, the content of various phospholipids in the user's breast milk can be determined through the historical data and the dietary structure, and further based on the content and the breastfeeding amount (the breastfeeding amount is the amount of breast milk used for breastfeeding in a single day, and the user can use a bottle to collect the breast milk to determine the amount of breast milk), the amount of various phospholipids that the user can provide to the baby through breastfeeding can be determined, thereby obtaining a phospholipid supply curve; In this embodiment, the established milk powder phospholipid content database includes each kind of milk powder and the content of each kind of phospholipid corresponding to the milk powder; through the data in the database and the milk powder usage of the user, the increment that each kind of milk powder can bring to the phospholipid supply curve can be determined, and after the phospholipid supply curve is adjusted for the increment brought by each kind of milk powder, the matching milk powder can be determined, that is, the initial section of the phospholipid supply curve can be made to be within the preset phospholipid content range (a phospholipid content range is preset for each kind of phospholipid, and the initial section of the phospholipid supply curve corresponding to each phospholipid is required to be within the corresponding phospholipid content range); the initial section is a section on the phospholipid supply curve corresponding to a set time (for example, one month) from the beginning of the nursing cycle; if there are multiple milk powders that can make the initial sections all within the corresponding phospholipid content range, the milk powder corresponding to the latest time point corresponding to the first over-range of the phospholipid supply curve is selected as the matching milk powder; through the feeding method corresponding to the phospholipid supply curve (that is, according to the breastfeeding method given by the user), the matching milk powder is selected. The amount and milk powder content are used to feed the baby with breast milk and matching milk powder), so that the supply of various phospholipids to the baby by the user in the time period corresponding to the initial section is within the corresponding phospholipid content range; further, the phospholipid supply curve often cannot be within the preset content range throughout the entire lactation cycle (the duration from delivery to cessation of lactation, the historical statistical mean is taken in this embodiment, such as 2 years). At this time, the present embodiment can also identify the out-of-range section in the phospholipid supply curve, and update the feeding method (such as updating the breastfeeding amount and milk powder dosage or changing the milk powder) at the adjustment time point corresponding to the out-of-range section (that is, the time point corresponding to the starting point of the out-of-range section) to make the out-of-range section return to the preset content range, so that the phospholipid supply to the baby is always kept within a reasonable range; after the adjustment is completed, the corresponding new feeding method is marked at the position of the phospholipid supply curve corresponding to the adjustment time point, and then the phospholipid supply curve is sent to the user end, so that the user can intuitively know what feeding method should be used at what time.

[0019] In the present application, the phospholipid content in the user's breast milk can be predicted, so as to determine the matching milk powder for the user, so that the user can use the matching milk powder and her own breast milk for feeding, provide a reasonable amount of phospholipids for the baby, and ensure the healthy growth of the baby. Moreover, since the phospholipid content in the user's breast milk will change during the lactation cycle, the present application can predict the changing trend of the phospholipid supply caused by this change during the lactation cycle, so as to identify the time period when the phospholipid supply exceeds the range, and give corresponding feeding method adjustment suggestions for this time period, so that the phospholipid supply provided by the user to the baby throughout the lactation cycle is maintained at a reasonable level.

[0020] As a preferred embodiment, the content of various phospholipids in each milk powder is determined by liquid chromatography-tandem mass spectrometry, including: Preparation of the assay solution: weigh 2 g of the milk powder sample in a 50 mL centrifuge tube, add 20 mL of water, and record the total mass of the milk powder sample and water; after vortex mixing, heat the sample solution in a 40°C constant temperature shaking water bath for 30 min; weigh 0.5 g of the sample solution in a 15 mL centrifuge tube, add 0.9 mL of pure water and then add 5 mL of dichloromethane-methanol solution, mix well, place the centrifuge tube on an ultrasonic cleaner for ultrasonic cleaning at 40°C for 10 min, place the centrifuge tube on a centrifuge for centrifugation at 8000 r / min for 10 min, collect the lower layer liquid, add 5 mL of dichloromethane-methanol solution again, mix well, place the centrifuge tube on an ultrasonic cleaner for ultrasonic cleaning at 40°C for 10 min, place the centrifuge tube on a centrifuge for centrifugation at 8000 r / min for 10 min, collect the lower layer liquid, combine the lower layer liquids collected twice, pass through a 0.22 μm microporous filter membrane, dissolve and fix the volume with dichloromethane-methanol to obtain the assay solution.

[0021] The test solution is measured by liquid chromatography-tandem mass spectrometry to obtain the phospholipid concentration in the test solution; The liquid chromatography-tandem mass spectrometry instrument transmits the phospholipid concentration to the computer device, and the computer device calculates the phospholipid content of the sample based on the phospholipid concentration.

[0022] In this embodiment, the computer device calculates the content of any phospholipid in the milk powder by the following formula: Wherein, X is the content of the phospholipid, To determine the concentration of the phospholipid in the solution, m is the sample mass and V is the fixed volume of the solution to be measured.

[0023] In this embodiment, the unit of C is g / mL, the unit of V is mL, and the unit of m is g; different phospholipids are detected, and the corresponding isotope internal standard working solutions are also different. For example, if phosphatidylcholine is to be detected, the isotope internal standard working solution of phosphatidylcholine is pre-prepared, and the preparation steps are as follows: accurately weigh 10 mg of phosphatidylcholine standard (accurate to 0.00001 g), place it in a 10 mL brown volumetric flask, dissolve it with 0.1% formic acid methanol solution and dilute to the scale, prepare a standard stock solution with a concentration of 1000 μg / mL, and store it below -18°C; In this embodiment, before the measurement solution is put on the machine for measurement, a series of standard curve solutions of the phospholipids to be measured need to be prepared, including a plurality of solutions of phospholipids with standard concentrations from small to large (such as 0.5 μg / mL, 1 μg / mL, 1.5 μg / mL, etc.), which are measured on the machine in order from small to large concentrations, and the standard curve is drawn with the phospholipid concentration as the horizontal axis and the phospholipid isotope internal standard peak area as the vertical axis; then the measurement solution is measured to obtain the curve of the measurement solution, and the measurement solution curve is compared with the standard curve, so as to quantify the concentration of the measured substance in the measurement solution.

[0024] The chromatographic conditions tested included: Temperature: 50℃; Injection volume: 5 μL; Mobile phase A: isopropanol-acetonitrile (1:1); mobile phase B: 20 ​​mmol / L ammonium formate aqueous solution; Flow rate: 0.3 mL / min; Mass spectrometry conditions included: Ion source: electrospray ion source, temperature 250℃; Scanning mode: positive ion mode and negative ion mode; Collision gas: argon; Heating block temperature: 400°C Desolventizing temperature: 650℃; Desolventizing gas flow rate: 900L / h; Capillary voltage: 1.0 kV.

[0025] As a preferred embodiment, the dietary structure is the food variety and corresponding food intake of the user in a set dietary cycle in the past; the historical data includes the content of each phospholipid in the colostrum of lactating women under various dietary structures, and the average change ratio of various phospholipids at each preset time point in the lactation cycle; the phospholipid content change curve in the breast milk of the user during the lactation cycle determined according to the historical data and the dietary structure includes: S31: comparing the user's dietary structure with each dietary structure in the historical data to determine the first content of each phospholipid in the user's colostrum; S32: For each phospholipid, a first coordinate system is generated, wherein the abscissa of the first coordinate system is the lactation cycle, and the ordinate is the content of the phospholipid; S33: determining the second content of the phospholipid at each preset time point in the lactation cycle according to the first content of the phospholipid and the corresponding average change ratio, and obtaining a coordinate point corresponding to each preset time point, wherein the abscissa of the coordinate point is the preset time point, and the ordinate is the corresponding second content; S34: marking the coordinate points in the first coordinate system, and connecting the coordinate points with a smooth curve to obtain a content variation curve of the phospholipid in the user's breast milk.

[0026] In this embodiment, the starting point of the horizontal axis of the first coordinate system is the starting point of the nursing cycle; the historical data is the data actually detected in the past; for example, the phospholipid content in the colostrum of lactating women under each dietary structure is detected and stored for subsequent use; for the average change ratio at each preset time point in the lactation cycle, the change ratio of each preset time point of each lactating woman is first detected, and for each preset time point, the change ratio of each lactating woman corresponding to the preset time point is averaged to obtain the average change ratio; the step of detecting the change ratio of each preset time point of each lactating woman (for example, 1 month, 2 months, 3 months, etc. from delivery) is: first detect the various phospholipid contents in the colostrum of the lactating woman, and detect the various phospholipid contents in the breast milk of the woman once at each preset time point, and divide the detected content of each phospholipid by the content of the corresponding phospholipid in the colostrum to obtain the change ratio of the phospholipid at the preset time point.

[0027] As a preferred embodiment, comparing the user's dietary structure with each dietary structure in the historical data to determine the first content of each phospholipid in the user's colostrum includes: For each phospholipid, determine the associated elements of the phospholipid and the associated food types of the associated elements; Calculating a first intake of the associated element by the user according to the amount of the associated food variety consumed by the user; Calculate the second intake of related elements for each lactating woman in historical data; Selecting a second intake amount greater than the first intake amount and a second intake amount less than the first intake amount, and identifying the gap ratio of the first intake amount between the two second intake amounts; The difference between the contents of the phospholipid corresponding to the two second intakes is calculated, and the first content is determined according to the difference and the intervening ratio.

[0028] In this embodiment, the reason why a certain phospholipid exists in breast milk is that the human body absorbs the associated element for synthesizing the phospholipid. In order to obtain the associated element, the human body needs to eat the associated food including the associated element. In this embodiment, a phospholipid-associated element-associated food type comparison table is preset, which includes the associated element of each phospholipid and the food variety that can provide the associated element (including the unit intake of the associated element corresponding to the unit intake of the food variety). Then, the first intake can be obtained by multiplying the unit intake by the user's intake of the corresponding associated food type. The second intake of the associated element for each lactating woman in the historical data can be obtained in the same way. Amount; For the interstitial ratio, for example, the first intake is 2g, the selected second intake is 1g, and the other second intake is 3g. The difference between the two second intakes is 3-1=2g, and the difference between the first intake and the smaller second intake is 2-1=1g, and the interstitial ratio is 1 / 2; further, the phospholipid content corresponding to the smaller second intake is 0.3%, and the phospholipid content corresponding to the other second intake is 0.9%, and the content difference is 0.9%-0.3%=0.6%. Multiply 0.6% by the interstitial ratio to obtain the interstitial value of 0.3%, and then add the interstitial value to the smaller second intake to obtain 0.6%, which is the first content.

[0029] As a preferred embodiment, colostrum is breast milk at the beginning of a lactation cycle, the preset time point corresponding to the colostrum is the starting point of the lactation cycle, and the average change ratio is the ratio of a phospholipid content in breast milk at the preset time point to the corresponding phospholipid content in colostrum; Determining the second content of the phospholipid at each preset time point during the lactation cycle according to the first content of the phospholipid and the corresponding average change ratio includes: For each phospholipid, the average change ratio of the phospholipid corresponding to each time point is retrieved; The first content is multiplied by the average change ratio of each adjustment to obtain the second content corresponding to each time point.

[0030] In this embodiment, the average change ratio of the preset time points corresponding to colostrum is 1; through this embodiment, the phospholipid content at each stage of the nursing cycle can be calculated, thereby obtaining a change curve of the phospholipid content, which can intuitively reflect the change trend of the phospholipid content in the nursing cycle.

[0031] As a preferred embodiment, determining the phospholipid supply curve according to the lactation amount and the phospholipid content change curve includes: Corresponding to each first coordinate system, a second coordinate system is generated, wherein the abscissa of the second coordinate system is the nursing period and corresponds to the abscissa of the first coordinate system, and the ordinate is the corresponding phospholipid supply; Multiply the ordinate of each coordinate point of the phospholipid content change curve in the first coordinate system by the lactation amount to obtain the corresponding phospholipid supply, and then obtain a coordinate point in the second coordinate system; The coordinate points on each second coordinate system are connected with a smooth curve to obtain a phospholipid supply curve.

[0032] In this embodiment, the amount of phospholipids that can be provided to the infant through breast milk feeding can be obtained by multiplying the phospholipid content in breast milk by the amount of breastfeeding. The phospholipid content corresponding to each point on the phospholipid content curve is multiplied by the amount of breastfeeding to obtain several supply amounts, and then the corresponding phospholipid supply curve can be obtained.

[0033] As a preferred embodiment, determining the matching milk powder of the user from the milk powder phospholipid content database includes: S51: selecting a milk powder from a milk powder phospholipid content database as an alternative milk powder; S52: for each phospholipid, multiply the content of the phospholipid in the alternative milk powder by the amount of milk powder to obtain the increased supply of the phospholipid; S53: in the second coordinate system corresponding to the phospholipid, simulating the phospholipid supply curve to increase the supply increase; S54: generating a first horizontal line and a second horizontal line on the second coordinate system, wherein the first horizontal line corresponds to a preset minimum content of the phospholipid, and the second horizontal line corresponds to a preset maximum content of the phospholipid; S55: identifying a curve point on the phospholipid supply curve that is lower than the first horizontal line for the first time or higher than the second horizontal line for the first time, and then identifying the abscissa of the curve point as the limit-exceeding time point; S56: determining the earliest time point from the over-limit time points corresponding to each phospholipid as the target time point of the candidate milk powder; S57: Repeat steps S51 to S56 until the target time point of each candidate milk powder is obtained, and determine the candidate milk powder with the latest corresponding target time point as the matching milk powder; The phospholipid supply curve is adjusted, that is, each phospholipid supply curve is improved as a whole to match the increased supply of phospholipids corresponding to the milk powder.

[0034] In this embodiment, for each type of milk powder, before the corresponding target time point, the milk powder makes the phospholipid supply curve of each type of phospholipid within a reasonable range (i.e., between the first horizontal line and the second horizontal line). The later the target time point is, the longer the currently determined feeding method can last, and the frequency of subsequent adjustment of the feeding method can be reduced as much as possible; the simulation is improved until the corresponding target time point is determined, and then the phospholipid supply curve is reset, and then the target time point determination process of another milk powder is carried out; after the matching milk powder is finally determined, each phospholipid supply curve is improved as a whole by the increase in the supply of phospholipids corresponding to the matching milk powder, and this improvement is not reset.

[0035] like Figure 3 As shown, as a preferred embodiment, identifying the out-of-range section of the phospholipid supply curve, adjusting the feeding method according to the out-of-range section, and adjusting the phospholipid supply curve accordingly include: S61: determining the earliest time point among the over-limit time points of each phospholipid supply curve as the adjustment time point; S62: changing the dosage ratio of the breastfeeding amount and the milk powder dosage after the adjustment time point, so that the sections of the phospholipid supply curves after the adjustment time point are adjusted accordingly; S63: Determine whether the adjustment time point can be postponed by adjusting the dosage ratio. If so, determine the dosage ratio that postpones the adjustment time point for the longest time as the target ratio, adjust the section of the phospholipid supply curve after the adjustment time point according to the target ratio, and then determine a new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the nursing cycle; S64: If not, re-determine the matching milk powder for the segment after the adjustment time point, and adjust the segment after the adjustment time point accordingly to obtain a new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the feeding cycle.

[0036] Determining whether the adjustment time point can be postponed by adjusting the dosage ratio includes: S631: Determine all dosage ratios; S632: selecting a dosage ratio, for each phospholipid supply curve, adjusting the section of the phospholipid supply curve after the adjustment time point according to the dosage ratio, and identifying the over-limit time point of the section after adjustment; S633: determining the earliest time point of the over-limit time points corresponding to each phospholipid supply curve, identifying whether the earliest time point is later than the adjustment time point, and if so, determining whether the adjustment time point can be postponed by adjusting the dosage ratio; S634: If not, execute steps S632 to S634 until it is determined that the adjustment time point can be postponed by adjusting the dosage ratio, or if the earliest time point cannot be made later than the adjustment time point, it is determined that the adjustment time point cannot be postponed by adjusting the dosage ratio.

[0037] In this embodiment, since the baby's food intake is fixed, the breastfeeding amount increases and the amount of milk powder decreases accordingly; the steps of determining all dosage ratios are: first determine the equivalent breastfeeding amount per unit of milk powder; determine the breastfeeding amount when only breast milk is used (the amount of milk powder is 0 at this time); then increase the unit milk powder amount for the milk powder, reduce the equivalent breastfeeding amount for the breastfeeding amount, and then calculate the ratio of the breastfeeding amount to the milk powder amount, then a dosage ratio is obtained, and this step is repeatedly performed until the breastfeeding amount is reduced to 0, and all dosage ratios can be obtained; in this timely and appropriate amount, after determining the target ratio, the breastfeeding amount and milk powder amount corresponding to the target ratio are used to recalculate the phospholipid supply corresponding to the breastfeeding at each subsequent preset time node and the supply increase corresponding to the milk powder (the specific steps are consistent with the above and are not repeated here), so that an updated phospholipid supply curve can be obtained (only updated after the adjustment time point).

[0038] In this embodiment, each adjustment of the phospholipid supply curve is to adjust the section after the adjustment time point, that is, the user is informed that the feeding method can only be adjusted after the adjustment time point, and the dosage ratio is indicated at the adjustment time point to inform the user how to adjust specifically; when the milk powder needs to be re-matched, the newly matched milk powder type is also marked at the corresponding adjustment time point to inform the user that the milk powder needs to be changed at this time point to meet the baby's phospholipid needs.

[0039] The technical features of the above-described embodiments may be arbitrarily combined. To make the description concise, not all possible combinations of the technical features in the above-described embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0040] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for detecting and analyzing phospholipids in infant formula milk powder, characterized in that: The method comprises: S1: Determine the content of various phospholipids in each type of milk powder by liquid chromatography-tandem mass spectrometry, and establish a milk powder phospholipid content database based on the determination results; S2: When receiving an analysis request from any user, inquire about the user's diet structure and breastfeeding information, wherein the breastfeeding information includes the amount of breastfeeding and the amount of milk powder used; S3: Determine the phospholipid content change curve in the user's breast milk during the lactation cycle based on historical data and dietary structure; S4: Determine the phospholipid supply curve based on the lactation amount and the phospholipid content change curve; S5: determining the matching milk powder of the user from the milk powder phospholipid content database, and determining the phospholipid supply increase amount corresponding to the matching milk powder, so as to adjust the phospholipid supply curve so that the initial section of the phospholipid supply curve is within a preset phospholipid content range; S6: identifying the out-of-range section of the phospholipid supply curve, adjusting the feeding method according to the out-of-range section, and adjusting the phospholipid supply curve accordingly, so that the phospholipid supply curve is always within the preset phospholipid content range; S7: Send the adjusted phospholipid supply curve, the corresponding adjustment time point and the adjusted feeding method to the user.

2. The method according to claim 1, characterized in that The content of various phospholipids in each milk powder was determined by liquid chromatography-tandem mass spectrometry including: Preparation of the assay solution: weigh 2 g of the milk powder sample in a 50 mL centrifuge tube, add 20 mL of water, and record the total mass of the milk powder sample and water; after vortex mixing, heat the sample solution in a 40°C constant temperature shaking water bath for 30 min; weigh 0.5 g of the sample solution in a 15 mL centrifuge tube, add 0.9 mL of pure water and then add 5 mL of dichloromethane-methanol solution, mix well, place the centrifuge tube in an ultrasonic cleaner for ultrasonic cleaning at 40°C for 10 min, place the centrifuge tube in a centrifuge for centrifugation at 8000 r / min for 10 min, collect the lower layer liquid, add 5 mL of dichloromethane-methanol solution again, mix well, place the centrifuge tube in an ultrasonic cleaner for ultrasonic cleaning at 40°C for 10 min, place the centrifuge tube in a centrifuge for centrifugation at 8000 r / min for 10 min, collect the lower layer liquid, combine the lower layer liquids collected twice, pass through a 0.22 μm microporous filter membrane, and dissolve and fix the volume with dichloromethane-methanol to obtain the assay solution; The test solution is measured by liquid chromatography-tandem mass spectrometry to obtain the phospholipid concentration in the test solution; The liquid chromatography-tandem mass spectrometry instrument transmits the phospholipid concentration to the computer device, and the computer device calculates the phospholipid content of the sample based on the phospholipid concentration.

3. The method according to claim 1, characterized in that The dietary structure refers to the food types and corresponding food intake of the user in a set dietary cycle in the past; the historical data includes the content of various phospholipids in the colostrum of lactating women under various dietary structures, and the average change ratio of various phospholipids at each preset time point in the lactation cycle; the phospholipid content change curve of the user's breast milk during the lactation cycle determined based on the historical data and dietary structure includes: S31: comparing the user's dietary structure with each dietary structure in the historical data to determine the first content of each phospholipid in the user's colostrum; S32: For each phospholipid, a first coordinate system is generated, wherein the abscissa of the first coordinate system is the lactation cycle, and the ordinate is the content of the phospholipid; S33: determining the second content of the phospholipid at each preset time point in the lactation cycle according to the first content of the phospholipid and the corresponding average change ratio, and obtaining a coordinate point corresponding to each preset time point, wherein the abscissa of the coordinate point is the preset time point, and the ordinate is the corresponding second content; S34: marking the coordinate points in the first coordinate system, and connecting the coordinate points with a smooth curve to obtain a content variation curve of the phospholipid in the user's breast milk.

4. The method according to claim 3, characterized in that Comparing the user's dietary structure with each dietary structure in the historical data to determine the first content of each phospholipid in the user's colostrum includes: For each phospholipid, determine the associated elements of the phospholipid and the associated food types of the associated elements; Calculating a first intake of the associated element by the user according to the amount of the associated food variety consumed by the user; Calculate the second intake of related elements for each lactating woman in historical data; Selecting a second intake amount greater than the first intake amount and a second intake amount less than the first intake amount, and identifying the gap ratio of the first intake amount between the two second intake amounts; The difference between the contents of the phospholipid corresponding to the two second intakes is calculated, and the first content is determined according to the difference and the intervening ratio.

5. The method according to claim 3, characterized in that: Colostrum is breast milk at the beginning of the lactation cycle, the preset time point corresponding to colostrum is the starting point of the lactation cycle, and the average change ratio is the ratio of the content of a phospholipid in breast milk at the preset time point to the corresponding phospholipid content in colostrum; Determining the second content of the phospholipid at each preset time point during the lactation cycle according to the first content of the phospholipid and the corresponding average change ratio includes: For each phospholipid, the average change ratio of the phospholipid corresponding to each time point is retrieved; The first content is multiplied by the average change ratio of each adjustment to obtain the second content corresponding to each time point.

6. The method according to claim 3, characterized in that The phospholipid supply curve is determined based on the lactation amount and phospholipid content change curve, including: Corresponding to each first coordinate system, a second coordinate system is generated, wherein the abscissa of the second coordinate system is the nursing period and corresponds to the abscissa of the first coordinate system, and the ordinate is the corresponding phospholipid supply; Multiply the ordinate of each coordinate point of the phospholipid content change curve in the first coordinate system by the lactation amount to obtain the corresponding phospholipid supply, and then obtain a coordinate point in the second coordinate system; The coordinate points on each second coordinate system are connected with a smooth curve to obtain a phospholipid supply curve.

7. The method according to claim 6, characterized in that The matching milk powder for the user determined from the milk powder phospholipid content database includes: S51: selecting a milk powder from a milk powder phospholipid content database as an alternative milk powder; S52: for each phospholipid, multiply the content of the phospholipid in the alternative milk powder by the amount of milk powder to obtain the increased supply of the phospholipid; S53: in the second coordinate system corresponding to the phospholipid, simulating the phospholipid supply curve to increase the supply increase; S54: generating a first horizontal line and a second horizontal line on the second coordinate system, wherein the first horizontal line corresponds to a preset minimum content of the phospholipid, and the second horizontal line corresponds to a preset maximum content of the phospholipid; S55: identifying a curve point on the phospholipid supply curve that is lower than the first horizontal line for the first time or higher than the second horizontal line for the first time, and then identifying the abscissa of the curve point as the limit-exceeding time point; S56: determining the earliest time point from the over-limit time points corresponding to each phospholipid as the target time point of the candidate milk powder; S57: Repeat steps S51 to S56 until the target time point of each candidate milk powder is obtained, and determine the candidate milk powder with the latest corresponding target time point as the matching milk powder; The phospholipid supply curve is adjusted, that is, each phospholipid supply curve is improved as a whole to match the increased supply of phospholipids corresponding to the milk powder.

8. The method according to claim 7, characterized in that Identify the out-of-range section of the phospholipid supply curve, adjust the feeding method according to the out-of-range section, and adjust the phospholipid supply curve accordingly, including: S61: determining the earliest time point among the over-limit time points of each phospholipid supply curve as the adjustment time point; S62: changing the dosage ratio of the breastfeeding amount and the milk powder dosage after the adjustment time point, so that the sections of the phospholipid supply curves after the adjustment time point are adjusted accordingly; S63: Determine whether the adjustment time point can be postponed by adjusting the dosage ratio. If so, determine the dosage ratio that postpones the adjustment time point for the longest time as the target ratio, adjust the section of the phospholipid supply curve after the adjustment time point according to the target ratio, and then determine a new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the nursing cycle; S64: If not, re-determine the matching milk powder for the segment after the adjustment time point, and adjust the segment after the adjustment time point accordingly to obtain a new adjustment time point, and execute steps S62 to S63 until the adjustment time point is postponed to the end of the feeding cycle.

9. The method according to claim 7, characterized in that: Determining whether the adjustment time point can be postponed by adjusting the dosage ratio includes: S631: Determine all dosage ratios; S632: selecting a dosage ratio, for each phospholipid supply curve, adjusting the section of the phospholipid supply curve after the adjustment time point according to the dosage ratio, and identifying the over-limit time point of the section after adjustment; S633: determining the earliest time point of the over-limit time points corresponding to each phospholipid supply curve, identifying whether the earliest time point is later than the adjustment time point, and if so, determining whether the adjustment time point can be postponed by adjusting the dosage ratio; S634: If not, execute steps S632 to S634 until it is determined that the adjustment time point can be postponed by adjusting the dosage ratio, or if the earliest time point cannot be made later than the adjustment time point, it is determined that the adjustment time point cannot be postponed by adjusting the dosage ratio.

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