Method for delaying ripening of waxberry fruits and improving flavor of fruits by using far-red light

Abstract

The invention relates to the technical field of laser planting, in particular to a method for delaying ripening of waxberry fruits and improving the flavor of the fruits through far-red light. The invention provides a method for delaying ripening of waxberry fruits and improving fruit flavor by far-red light, which comprises the following steps: in a waxberry tree cultivation greenhouse, continuously supplementing 720-740nm far-red light at 6: 00-18: 00 every day from the development period to the mature period of the waxberry fruits to complete the cultivation of waxberry trees; the far-red light with the specific wave band is used for supplementing light in the period from the growth period to the mature period of the waxberry fruits, so that the treated waxberries show delayed maturation of the fruits, and the flavor and the nutritional value of the fruits are remarkably improved.

Description

Technical Field

[0001] The invention relates to the field of laser planting technology, and in particular to a method for delaying the ripening of bayberry fruits and improving the flavor of the fruits using far-infrared light. Background Art

[0002] Bayberry (Myrica rubra) is a subtropical evergreen fruit tree of the Myricaceae family, mainly distributed in southern China and other Asian countries. The fruit is highly sought after for its unique flavor and nutritional value, and has extremely high economic value. Bayberry fruit is rich in anthocyanins and other flavonoids, which are important secondary metabolites in plants. They not only regulate the plant's own physiological functions such as resistance to ultraviolet rays, high temperature, and drought, but also provide humans with rich nutritional and medicinal resources, which can help people prevent the occurrence of various diseases.

[0003] Light not only provides effective radiation energy for photosynthesis, but also acts as an environmental signal to regulate plant growth and development, such as light morphogenesis. People have been looking for a suitable light source that can improve the quality and yield of plants. Light radiation with a wavelength of 300 to 800 nm is called effective radiation required for plant growth, and radiation with a wavelength of 380 to 710 nm is effective radiation for plant photosynthesis. Experiments have shown that light sources with different wavelength ranges have different effects on plant growth. Chlorophyll has the highest absorption of red and blue light, which can effectively promote the growth and development of plants.

[0004] The application of existing LED light sources in agricultural lighting supplementation shows that red light (660nm) can inhibit internode elongation, increase tillering, promote the formation of photosynthetic pigments and the accumulation of sugar substances, and blue light (480nm) has the effect of inhibiting stem elongation, promoting photosynthesis, and root development. The addition of far-red light (730nm, non-photosynthetically active radiation) usually affects the morphological construction and periodic rhythm of plants, resulting in the phenotype of elongation and reduced branching of plants, while increasing the distribution of assimilated products transported to the stems, and promoting fruit growth by increasing the dry mass distribution of fruits. Therefore, far-red light supplementation has a wide range of application potential in plant cultivation fields such as improving crop quality.

[0005] Compared with traditional plant lighting sources (incandescent lamps, fluorescent lamps, metal halide lamps), LED plant growth lamps have the characteristics of energy saving, long life, high efficiency, and easy regulation, and are widely used in the field of facility agriculture. The application of LED light source far-infrared light directly for supplementary lighting in the existing agricultural planting field is relatively rare, and is usually used to regulate plant flowering and hypocotyl elongation. Fruit trees have a long growth cycle, and short-term far-infrared light irradiation is difficult to change the plant morphology, but it can effectively increase the quality of the fruit. For this reason, the researchers of the present invention aim to develop a far-infrared lamp to supplement the light of bayberry trees during the fruit growth period, thereby improving the flavor, nutritional value and market economic value of bayberry fruit. Summary of the invention

[0006] The present invention provides a method for delaying the ripening of bayberry fruit and improving the flavor of the fruit by using far-infrared light. The method uses far-infrared light of a specific wavelength band to supplement the light during the development period to the ripening period of the bayberry fruit, so that the treated bayberry fruit shows delayed ripening, and the fruit flavor and nutritional value are significantly improved.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] The invention provides a method for delaying the ripening of bayberry fruits and improving the flavor of the fruits by using far-infrared light. In a bayberry tree cultivation greenhouse, far-infrared light of 720 to 740 nm is continuously added from 6:00 to 18:00 every day during the development period to the ripening period of the bayberry fruits to complete the cultivation of the bayberry trees.

[0009] The present invention utilizes far-infrared light to supplement light during the critical period of waxberry fruit ripening, that is, from the development period to the ripening period, so as to make the waxberry fruit ripen later, thereby increasing the time for accumulating nutrients and helping to improve the flavor of the fruit. From an economic perspective, controlling a batch of waxberries to ripen late and staggering their sales periods can reduce the fruit loss rate.

[0010] The fruit maturity period of the bayberry tree cultivated according to the method provided by the present invention is delayed by 3 to 5 days, and anthocyanins and amino acids are effectively accumulated in the bayberry fruits obtained, thereby improving the fruit flavor and nutritional value, and enhancing the commercial value of the bayberry fruits.

[0011] Preferably, far-red light of 725-735 nm is continuously added from 6:00 to 18:00 every day during the development period to the maturity period of the bayberry fruit.

[0012] Preferably, far-red light of 728-733 nm is continuously added from 6:00 to 18:00 every day during the development period to the maturity period of the bayberry fruit.

[0013] Preferably, the ambient light intensity of the bayberry cultivation greenhouse is ≤200 μmol·m -2 ·s -1 , ambient temperature is 20℃~35℃, and ambient humidity is 50%~90%.

[0014] Preferably, the daylight period and nightlight period of the bayberry cultivation greenhouse are 12 hours each, and the daylight period is 6:00-18:00.

[0015] Preferably, the far-infrared light intensity is 20 to 50 μmol·m -2 ·s -1 .

[0016] Further preferably, the far-infrared light intensity is 25 to 50 μmol·m-2 ·s -1 .

[0017] Preferably, the far-infrared light is provided by an LED far-infrared lamp.

[0018] Preferably, the far-red light is arranged in such a way that a single row of LED far-red lights is arranged on the shaded side of the bayberry tree, so that the far-red light irradiates the bayberry tree vertically, and the irradiation range of the far-red light is 1.5 to 3 m. 2 .

[0019] Preferably, the straight-line distance between the LED far-red lamp and the outermost fruit of the bayberry tree is 0.5 to 1.5 m.

[0020] Preferably, the current of the LED far-red lamp is adjusted weekly to ensure that the light intensity received by the outermost fruit of the bayberry tree is 20 to 50 μmol·m -2 ·s -1 .

[0021] Preferably, the current of the LED far-red lamp is adjusted weekly to ensure that the light intensity received by the outermost fruit of the bayberry tree is 25 to 50 μmol·m -2 ·s -1 .

[0022] As the bayberry tree grows, the distance between the bayberry fruit and the LED far-infrared lamp will decrease, so the far-infrared radiation intensity of the bayberry fruit will increase. In view of this natural growth situation, the applicant adjusts the current of the LED far-infrared lamp every week to ensure that the radiation intensity of all fruits can reach the required value within a certain range.

[0023] The far-infrared light used in this application has an adjustable bulb current parameter within a range of 0 to 0.6 A. By adjusting the bulb current, the light intensity value after adjusting the current is determined based on the measurement result of the far-infrared light in the spectrometer.

[0024] Preferably, the height of the LED far red lamp from the ground is 1.5 to 2 m, and the total length of a single row of LED far red lamps is 0.5 to 2 m.

[0025] The total length of the single-row LED far-red light can be adjusted by adding or subtracting bulbs. It is easy to operate and can effectively control the irradiation range.

[0026] Preferably, during the cultivation of the bayberry trees, daily management is carried out normally; the daily management includes water and fertilizer management, flower and fruit thinning, weeding and pest control.

[0027] Therefore, the present invention has the following beneficial effects:

[0028] (1) The present invention provides a light supplementation method, which uses far-infrared light of a specific wavelength band to supplement the light during the growth and maturity period of bayberry fruit, so that the treated bayberry shows delayed fruit maturity, and the fruit flavor and nutritional value are significantly improved.

[0029] (2) The method provided by the present invention can delay the ripening period of bayberry fruit by 3 to 5 days, and at the same time promote the accumulation of anthocyanins and amino acids in bayberry fruit, thereby improving the flavor and nutritional value of the fruit and enhancing the commercial value of bayberry fruit.

[0030] (3) The special far-infrared light irradiation mode set by the present invention helps the bayberry tree receive effective and stable far-infrared light irradiation, promotes the accumulation of bayberry fruit flavor substances and delays the fruit ripening time. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] Figure 1 This is a site view of an embodiment of the present invention, taken on February 27, 2024 when the far-red light was installed;

[0032] Figure 2 Comparative photos of bayberry fruits taken when the far-red light field was installed in the early stage of development of the embodiment of the present invention and the comparative example, taken on May 23, 2024 (mid-color change of bayberry);

[0033] Figure 3 This is the design diagram of the far red light of the present invention;

[0034] Figure 4 This is a graph showing the far-red light wavelength test results of Example 1;

[0035] Figure 5 It is a comparison diagram of the color change of bayberry fruit in Example 1 and Comparative Example 1;

[0036] Figure 6 It is a comparison diagram of the color change of bayberry fruit in Example 2 and Comparative Example 1;

[0037] Figure 7 This is a graph showing the determination of the substance content of bayberry fruit, where a is the total sugar content, b is the total acid content, c is the total phenol content, d is the total amino acid content, e is the total anthocyanin content, and f is the total flavonoid content. DETAILED DESCRIPTION

[0038] The present invention is further described below in conjunction with specific embodiments. Those of ordinary skill in the art will be able to implement the present invention based on these descriptions. In addition, the embodiments of the present invention involved in the following description are generally only embodiments of a part of the present invention, rather than all embodiments. Therefore, based on the embodiments in the present invention, all other embodiments obtained by those of ordinary skill in the art without making creative work should fall within the scope of protection of the present invention.

[0039] [Example]

[0040] Example 1

[0041] S1.LED far-infrared light field layout

[0042] LED far-infrared lamps are arranged in the cultivation greenhouse of Myrica rubra cv. DongKui Orient Pearl. The LED far-infrared lamps with a lamp holder length of 1m are installed on the supporting iron frame of the cultivation greenhouse 2m above the ground. The straight-line distance between the LED far-infrared lamp and the outermost fruit of the bayberry tree is kept at 0.7m. During installation, pay attention to adjusting the radiation angle of the far-infrared light source to make the light source as evenly distributed as possible. A single row of 9 LED far-infrared lamps are set on the shady side of the bayberry tree so that the far-infrared light irradiates the bayberry tree vertically. The irradiation range of the LED far-infrared lamp is 2m 2 , ensuring that one side of the bayberry tree can be effectively and stably irradiated by far-infrared light. In February 2024, a total of 10 bayberry trees were cultivated in the cultivation greenhouse, and each bayberry tree was arranged with LED far-infrared light field according to the above operation.

[0043] S2. Bayberry tree cultivation

[0044] The ambient light intensity of the cultivation greenhouse is ≤200 μmol·m -2 ·s -1 , ambient temperature 20-35℃, ambient humidity 50-90% (the far-red light regulation test of bayberry trees in this scheme has a long time span, so the span of ambient temperature and ambient humidity is large, and it is necessary to meet the ambient temperature and humidity requirements in different seasons. The specific real-time temperature and real-time humidity are affected by seasonal climate), the day and night photoperiod is 12h each, and 6:00-18:00 is the daylight period. During the development and maturity of bayberry fruits, the far-red light (730nm) supplementary light intensity is 50μmol·m -2 ·s -1 , uninterrupted supplementary lighting from 6:00 to 18:00 every day. Adjust the current of the LED far-red light once a week, according to the results of the spectrometer (such as Figure 4 The light intensity at the outermost fruit of the bayberry tree is kept constant at 50 μmol·m -2 ·s -1 It is recorded as FR50.

[0045] During the growth period of the fruit trees, daily management such as water and fertilizer management, flower and fruit thinning, weeding, and pest control are all carried out normally. The specific management conditions are as follows:

[0046] Water and fertilizer management: According to the soil moisture sensor data, when the soil moisture is lower than 25%, the fully automatic drip irrigation system is turned on and 50kg of water is irrigated per plant.

[0047] The types and proportions of fertilizers are mainly organic fertilizers, supplemented by inorganic fertilizers. The organic fertilizer is mainly sheep manure; the inorganic fertilizer is a compound fertilizer with a nitrogen: phosphorus: potassium mass ratio of 1:0.3:4.

[0048] Base fertilizer: In November every year, organic fertilizer is mainly applied, and 15kg to 20kg of decomposed sheep manure is applied per plant.

[0049] In mid to early May every year, apply fruit-enhancing fertilizer and 1kg to 2kg of compound fertilizer per plant.

[0050] Apply post-harvest fertilizer in June every year, with 10kg to 15kg of organic fertilizer per plant.

[0051] Weed control: Weed once a year before fruit picking (in May). Allow natural weeding, combine soil loosening and fertilization with manual weeding and mechanical mowing, and do not use herbicides for weeding.

[0052] Flower and fruit thinning: Manual flower thinning is carried out in early February and manual fruit thinning is carried out in March every year.

[0053] Example 2

[0054] The treatment in this embodiment is basically the same as that in embodiment 1, except that the far-red light (730 nm) supplementary light intensity is controlled to be 25 μmol·m during the development period to the maturity period of bayberry fruit. -2 ·s -1 , denoted as FR25.

[0055] Comparative Example 1

[0056] The treatment in this comparative example is basically the same as that in Example 1, except that natural light is used instead of far-infrared light, and no supplementary light treatment is performed, which is denoted as CK.

[0057] Bayberry fruits were collected from each group of Examples 1 to 2 and Comparative Example 1 at the early color change, mid-color change and mature stages, and randomly collected from each tree in each group. Six fruit samples were collected from each group for color change comparison. The results are shown in the figure. Figure 5 and Figure 6 As shown. Figure 5 and Figure 6 It can be observed that the ripening period of the fruits of the bayberry trees treated with far-infrared light was significantly delayed, and the ripening period was effectively delayed by 3 to 5 days.

[0058] The fully mature fruits in each group of Example 1 and Comparative Example 1 were randomly collected within the irradiation range of each tree in each group. 50 fruit samples were collected from each group for quality inspection and comparison. The results are shown in Tables 1 to 2 and Figure 7It can be seen from the observation that far-infrared light treatment can significantly reduce the acidity of bayberry fruit, with a decrease of 17.1%; the content of total phenol, total amino acid and total anthocyanin in the fruit increased significantly, with an increase of 17.3%, 77.9% and 9.4% respectively.

[0059] Table 1 Comparison of anthocyanin content in bayberry fruit

[0060]

[0061]

[0062] Table 2 Comparison of free amino acid content in bayberry fruit

[0063]

[0064] Figure 6 Determination method of total anthocyanins in:

[0065] After grinding the sample with liquid nitrogen, weigh about 0.1 g of the sample, add 1 mL of the extract, and after sufficient homogenization, cover tightly and perform ultrasonic extraction for 30 min, 8000g, centrifuge at room temperature for 10 min, and adjust the volume of the extract to 0.5 mL.

[0066] Maximum absorption wavelength determination: Take two portions of 0.04 mL of supernatant, add 0.16 mL of potassium chloride buffer solution to one portion and 0.16 mL of sodium acetate buffer solution to the other portion, let stand for 10 min, and perform full wavelength scan between 400 nm and 700 nm to determine the maximum absorption wavelength, A max =520nm.

[0067] Take two portions of 0.04 mL of the supernatant, add 0.16 mL of potassium chloride buffer solution and sodium acetate buffer solution respectively, mix well, let stand for 10 min, and measure the absorbance at wavelengths of 520 nm and 700 nm.

[0068] ΔA=pH 1.0(A520-A700)-pH 4.5(A520-A700),

[0069] Anthocyanin (μg / g) = [ΔA × V / (ε × d) × M1 × 10 6 ] / M2.

[0070] Where:

[0071] V—volume of extraction solution, 1×10 -3 L;

[0072] ε—molar extinction coefficient of anthocyanin, 2.69×10 4 L / mol / cm;

[0073] d—96-well plate light path, 0.6 cm;

[0074] M1—relative molecular mass of anthocyanin, 449.2 g / mol;

[0075] 10 6 —1g = 10 6 μg;

[0076] M2—sample mass, g.

[0077] Anthocyanin content determination method in Table 1:

[0078] 1. Weigh 0.1 g into a 2 mL centrifuge tube, add steel beads, add 1 mL of methanol: water solution (7:3, V / V, containing 0.1% formic acid) and vortex for 30 seconds;

[0079] 2. Homogenize at 45 Hz for 15 min and ultrasonicate for 30 min in an ice-water bath;

[0080] 3. Centrifuge the sample at 4°C, 12000r / min for 15min;

[0081] 4. Take 200 μL of the supernatant and filter it through a 0.22 μm filter membrane for analysis.

[0082] 1. Mobile phase conditions:

[0083] This project uses a Waters ACQUITY I-Class ultra-high performance liquid chromatograph to perform chromatographic separation of the target compounds using an ACQUITY UPLC HSST3 (100×2.1mm, 1.8μm, Waters) liquid chromatography column. The liquid chromatography phase A is an aqueous solution containing 0.1% formic acid, and the phase B is acetonitrile containing 0.1% formic acid. The column oven temperature is 35°C, the sample tray is set to 10°C, and the injection volume is 2μL.

[0084] 2. Mass spectrometry conditions:

[0085] This project used a SCIEX QTRAP 6500+ triple quadrupole mass spectrometer equipped with an IonDrive Turbo V ESI ion source to perform mass spectrometry analysis in multiple reaction monitoring (MRM) mode. The ion source parameters were as follows: Curtain Gas = 35 psi, Ion Spray Voltage = +5500 V, -4500 V, Temperature = 550 °C, Ion Source Gas 1 = 50 psi, Ion Source Gas 2 = 55 psi.

[0086] Before UHPLC-MS / MS analysis, the target compound standard solution was introduced into the mass spectrometer. For each target compound, several parent ion-daughter ion pairs (transitions) with the highest signal intensity were selected, and their MRM parameters were optimized. The ion pairs with the best response were selected for quantitative analysis, and the other ion pairs were used for qualitative analysis of the target compound.

[0087] In this project, all mass spectrometry data acquisition and quantitative analysis of target compounds were completed using SCIEX Analyst Work Station Software (Version 1.7.2) and Sciex OS 2.0.1.

[0088] The final measured concentration CF (Final Concentration, μg / L) of the sample is the concentration CC (Calculated Concentration, μg / L) directly measured by the instrument multiplied by the dilution factor Dil (Dilution Factor), and the unit is μg / L; the target metabolite concentration CM (Metabolite Concentration, ng / g) in solid samples or the target metabolite concentration CM (Metabolite Concentration, ng / mL) in liquid samples is equal to the final measured concentration CF of the sample multiplied by the final volume V (Volume, μL) of the sample and the concentration factor CF of the sample in the pretreatment, divided by the sample mass m (mass, mg or μL).

[0089] Solid sample calculation formula:

[0090] $$C_M[ng\cdot g^{-1}]=\frac{C_F[μg\cdot L^{-1}]\cdot V[μL]}{m[mg]}CF$$

[0091] Liquid sample calculation formula:

[0092] $$C_M[ng\cdot mL^{-1}]=\frac{C_F[μg\cdot L^{-1}]\cdot V[μL]}{m[μL]}CF$$

[0093] Figure 6 Total amino acid determination method:

[0094] After grinding the sample with liquid nitrogen, weigh about 0.1g of the sample, add 0.5mL of 10% acetic acid solution, fully homogenize, cover tightly and place in a boiling water bath for extraction for 15min; after cooling, centrifuge at 10000rpm, 4℃ for 10min, and take the supernatant for testing. Add 10μL of sample solution, 120μL of acetic acid-sodium acetate buffer solution, 100μL of 3% ninhydrin solution and 10μL of 0.3% ascorbic acid solution to the Ep tube in sequence. After mixing, cover the bottle cap tightly, place in a boiling water bath for 15min, cool and invert several times, centrifuge at 8000rpm for 5min, take the supernatant, and measure the absorbance at 570nm.

[0095] Draw a standard curve: dilute the 5 mg / mL leucine standard solution with distilled water to 0.80, 0.60, 0.50, 0.40, 0.30, 0.20, 0.10, 0.05, 0.00 mg / mL standard solutions, and measure the absorbance values ​​at different concentrations according to the operation of the sample solution. Draw a standard curve based on the relationship between concentration and absorbance.

[0096] Result calculation: free amino acid (mg / g) = C×V / M×F.

[0097] In the formula: C is the amino acid content calculated from the standard curve, mg / mL; V is the volume of the extract, 0.5mL; M is the sample mass, g; F is the dilution factor.

[0098] Determination method of free amino acids in Table 2:

[0099] 1 Metabolite extraction

[0100] 1. Weigh 0.1 g into a 2 mL centrifuge tube, add steel beads, add 1 mL of methanol: acetonitrile: water solution (2:2:1, V / V / V, containing isotope-labeled internal standard mixture) and vortex for 30 seconds;

[0101] 2. Homogenize at 45 Hz for 4 min, sonicate for 5 min in an ice-water bath, and repeat the homogenization and sonication steps 3 times;

[0102] 3. Stand at -20℃ for 1h;

[0103] 4. Centrifuge the sample at 4°C, 12000r / min for 15min;

[0104] 5. Take 200 μL of the supernatant and filter it through a 0.22 μm filter membrane for analysis.

[0105] 2. Preparation of standard solution

[0106] Accurately weigh the corresponding amount of standard into a volumetric flask and prepare a 1000ng / mL standard stock solution. Take the corresponding amount of standard stock solution into a 10mL volumetric flask and prepare a mixed standard solution. Dilute the standard solution in sequence to obtain a series of calibration solutions (containing an isotope-labeled internal standard mixture with the same final concentration as the sample).

[0107] 3. On-machine testing

[0108] 1. Mobile phase conditions:

[0109] This project uses a Waters ACQUITY I-Class ultra-high performance liquid chromatograph to perform chromatographic separation of the target compounds using an ACQUITY UPLC BEHAmide (150×2.1mm, 1.7μm, Waters) liquid chromatography column. The liquid chromatography phase A is an aqueous solution containing 0.2% formic acid, and the phase B is acetonitrile containing 0.2% formic acid. The column oven temperature is 35°C, the sample tray is set to 10°C, and the injection volume is 1μL.

[0110] 2. Mass spectrometry conditions:

[0111] This project used a SCIEX QTRAP 6500+ triple quadrupole mass spectrometer equipped with an IonDrive Turbo V ESI ion source to perform mass spectrometry analysis in multiple reaction monitoring (MRM) mode. The ion source parameters were as follows: Curtain Gas = 35 psi, Ion Spray Voltage = +5500 V, -4500 V, Temperature = 550 °C, Ion Source Gas 1 = 50 psi, Ion Source Gas 2 = 55 psi.

[0112] Before UHPLC-MS / MS analysis, the target compound standard solution was introduced into the mass spectrometer. For each target compound, several parent ion-daughter ion pairs (transitions) with the highest signal intensity were selected, and their MRM parameters were optimized. The ion pairs with the best response were selected for quantitative analysis, and the other ion pairs were used for qualitative analysis of the target compound.

[0113] In this project, all mass spectrometry data acquisition and quantitative analysis of target compounds were completed using SCIEX Analyst Work Station Software (Version 1.7.2) and Sciex OS 2.0.1.

[0114] 4 Target metabolite detection results in samples

[0115] The final measured concentration CF (Final Concentration, ng / mL) of the sample is the concentration CC (Calculated Concentration, ng / mL) directly measured by the instrument multiplied by the dilution factor Dil (Dilution Factor), and the unit is ng / mL; the target metabolite concentration CM (Metabolite Concentration, ng / g) in solid samples or the target metabolite concentration CM (Metabolite Concentration, ng / mL) in liquid samples is equal to the final measured concentration CF of the sample multiplied by the final volume V (Volume, μL) of the sample and the concentration factor CF of the sample in the pretreatment, divided by the sample weight m (mass, mg or μL).

[0116] Solid sample calculation formula:

[0117] CM[ng·g -1 ]=CF[ng·mL -1 ]·V[μL]m[mg]CFCM[ng·g -1 ]=CF[ng·mL -1 ]·V[μL]m[mg]CF

[0118] Liquid sample calculation formula:

[0119] CM[ng·mL -1 ]=CF[ng·mL -1 ]·V[μL]m[μL]CF

Claims

1. A method for delaying the ripening of bayberry fruit and improving the flavor of the fruit by using far-infrared light, characterized in that: In the bayberry cultivation greenhouse, far-red light of 720-740nm is continuously added from 6:00 to 18:00 every day during the development period to the maturity period of the bayberry fruit to complete the cultivation of the bayberry trees.

2. The method according to claim 1, characterized in that The ambient light intensity of the bayberry cultivation greenhouse is ≤200 μmol·m -2 ·s -1 , ambient temperature is 20℃~35℃, and ambient humidity is 50%~90%.

3. The method according to claim 1, characterized in that The daylight period and nightlight period of the bayberry cultivation greenhouse are 12 hours each, and the daylight period is 6:00-18:

00.

4. The method according to claim 1, characterized in that The far-red light intensity is 20 to 50 μmol·m -2 ·s -1 .

5. The method according to claim 1, characterized in that The far red light is provided by an LED far red light.

6. The method according to claim 1, 4 or 5, characterized in that: The far red light is set in a manner as follows: a single row of LED far red lights is set on the shaded side of the bayberry tree, so that the far red light irradiates the bayberry tree vertically, and the irradiation range of the far red light is 1.5 to 32 m。 7. The method according to claim 6, characterized in that The straight-line distance between the LED far-red lamp and the outermost fruit of the bayberry tree is 0.5 to 1.5 m.

8. The method according to claim 7, characterized in that The current of the LED far-red light was adjusted weekly to ensure that the light intensity received by the outermost fruit of the bayberry tree was 20-50 μmol·m -2 ·s -1 .

9. The method according to claim 6, characterized in that The height of the LED far red light from the ground is 1.5 to 2 m, and the total length of a single row of LED far red lights is 0.5 to 2 m.

10. The method according to claim 1, characterized in that During the cultivation period of the bayberry trees, daily management is carried out normally; the daily management includes water and fertilizer management, flower and fruit thinning, weeding and pest control.

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