Kinesio with massage particles and preparation method of kinesio

Abstract

The invention discloses a kinesio tape with massage particles and a preparation method thereof, and relates to the technical field of kinesio tapes, the kinesio tape sequentially comprises a tape layer, an adhesive layer, a particle adhesion layer and an isolation layer from top to bottom, and the side, opposite to the isolation layer, of the particle adhesion layer is provided with a plurality of massage particles. According to the kinesio patch with the massage particles and the preparation method of the kinesio patch, through the arrangement of the massage particles, hyaluronic acid between cells can be decomposed, medicine permeability is improved, and medicine contained in the patch layer can be better absorbed by skin; meanwhile, drug release can be delayed, so that the drug action time is prolonged, and the local effective drug concentration is maintained; a basis is provided for cell attachment and proliferation, and repair and regeneration of damaged muscle tissues are facilitated; the massage particles have the effects of resisting bacteria and promoting wound healing, can prevent skin infection possibly occurring in the use process of the patch, and are beneficial to recovery of muscle injury; the sticking effect of the patch is improved, and meanwhile, the irritation to the skin can be reduced.

Description

Technical Field

[0001] The invention relates to the technical field of kinesio patches, in particular to a kinesio patch with massage particles and a preparation method thereof. Background Art

[0002] Kinesiology Tape can be used to relieve muscle pain caused by overuse of certain specific muscles or sports injuries, as well as to accelerate muscle recovery. In addition, the tape can provide a lifting effect on the skin to improve blood circulation, thereby reducing muscle swelling and inflammation; in addition, it can reduce muscle soreness by reducing the burden of muscle contraction.

[0003] A Chinese patent with publication number CN221384040U discloses a Kinesio patch, which specifically relates to the field of sports medicine technology, and includes an elastic patch body, the patch body includes two anchor parts and a treatment part, the two ends of the treatment part are respectively fixedly connected to the two anchor parts, the width of the treatment part is greater than the width of the anchor part, and the contour lines of the two ends of the treatment part that are not connected to the two anchor parts are respectively a first arc line and a second arc line, and the first arc line and the second arc line are both convex arc lines. The utility model can better fit the human body structure, is convenient and easy to use, and further promotes the clinical use of Kinesio patch. However, the above-mentioned prior art solutions have the following defects: the prepared Kinesio patch has poor antibacterial properties when in use; for this reason, the present invention provides a Kinesio patch with massage particles and a preparation method thereof to solve the above-mentioned problems. Summary of the invention

[0004] In view of the deficiencies in the prior art, the present invention provides a kinesio patch with massage particles and a preparation method thereof, which solves the problems mentioned in the above background technology.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: a kinesio patch with massage particles, comprising, from top to bottom, a patch layer, an adhesive layer, a particle attachment layer and an isolation layer, wherein the particle attachment layer is provided with a plurality of massage particles on one side of the isolation layer, the isolation layer is provided with a plurality of air holes, and the massage particles pass through the air holes, the isolation layer is bonded to the surface of the particle attachment layer, the adhesive layer is coated on the surface of the patch layer, and release paper is attached to the other side during packaging, so that the patch layer, the particle attachment layer and the isolation layer are separately packaged, and a particle attachment layer with massage particles of different sizes is provided in the same package.

[0006] Preferably, the massage particles are made of gel material, and massage particles of different specifications are distributed on the same particle attachment layer in three forms: the same size, the same size, and mixed sizes.

[0007] Preferably, the specific preparation process of the massage particles is:

[0008] S1. Mix 1.5-2.5 g of hyaluronidase with 120 mL of deionized water, and add 0.5 g of vitamin C as an antioxidant to prevent the enzyme from being inactivated in subsequent steps; after stirring evenly, preheat in a 37°C constant temperature water bath for 15 minutes;

[0009] S2. Add 1.5-2 g of sodium periodate to the above solution, and stir at 200 rpm for 3 hours using a magnetic stirrer under conditions of complete light avoidance; after the reaction is completed, add 5 mL of a 10% sodium thiosulfate solution to neutralize the remaining sodium periodate to prevent it from interfering with subsequent steps;

[0010] S3, add 4-5mL of ethylene glycol and 0.2g of ethylenediaminetetraacetic acid to chelate the metal ions present to prevent them from affecting the gel properties, and continue stirring for 60 minutes;

[0011] S4, placing the reaction solution in deionized water, dialyzing using a dialysis bag with a molecular weight cutoff of 10 kDa, extending the dialysis time to 24 hours to ensure that small molecule impurities are fully removed, and then freeze-drying;

[0012] S5, adding 1-1.2 g of modified hydroxyapatite and 0.8-1.2 g of modified chitosan to the dried hyaluronidase-ethylene glycol mixture, and stirring for 30 minutes under ultrasonic stirring until completely dissolved; then stirring for another 30 minutes under ultrasonic stirring to form a uniform gel;

[0013] S6. Inject the gel into the mold and perform a freeze-thaw cycle to enhance the strength and stability of the gel. The freeze-thaw cycle is to first freeze to -20°C, keep it for 2 hours, then thaw it to room temperature, and repeat 3 times; finally, cut and shape it as needed to obtain the final massage particles.

[0014] Preferably, in S5, the preparation method of modified hydroxyapatite is: mixing Tris-hydrochloric acid buffer with anhydrous ethanol, adding hydroxyapatite, ultrasonic treatment for 35 minutes, then adding polydopamine solution, stirring for 24 hours in a dark environment, and finally freeze-drying to obtain modified hydroxyapatite.

[0015] Preferably, in the preparation process of the modified hydroxyapatite, the added amount of each component is: Tris-hydrochloric acid buffer 90-100 mL, ethanol 10-20 mL, hydroxyapatite 2-3.5 g, polydopamine 0.8-1.5 g, and the polydopamine solution is pre-dissolved in 10 mL of deionized water with a concentration of 10 mg / mL.

[0016] Preferably, in S5, the preparation method of the modified chitosan is: dissolving chitosan in an acetic acid solution, adding a 20% hydrochloric acid solution, deoxygenating for 20 minutes under a nitrogen atmosphere, and then adding gallic acid to obtain a solution one after complete dissolution; dissolving 1-ethyl-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide in deionized water to obtain a solution two; under a nitrogen atmosphere, mixing solution one with solution two, dialyzing for 24 hours using a dialysis bag with a molecular weight cutoff of 3.5 kDa, and then freeze-drying to obtain the modified chitosan.

[0017] Preferably, during the preparation of the modified chitosan, the added amounts of the components are: chitosan 2.5-5 g; acetic acid solution 4-7 ml, and the concentration of the acetic acid solution is 2%; deionized water 75-100 mL; hydrochloric acid solution 4-6 mL, and the concentration is 20%; gallic acid 3.5-6 g; 1-ethyl-(3-dimethylaminopropyl)carbodiimide 2.8-4.2 g; N-hydroxysuccinimide 1.6-2.5 g.

[0018] The invention also discloses a method for preparing a kinesio patch with massage particles. The specific preparation method is: bonding a patch layer and a particle attachment layer through an adhesive layer to prepare the kinesio patch with massage particles.

[0019] The present invention provides a kinesio patch with massage particles and a preparation method thereof. Compared with the prior art, the kinesio patch with massage particles and a preparation method thereof have the following beneficial effects: the kinesio patch with massage particles and a preparation method thereof can decompose hyaluronic acid between cells through the setting of massage particles, increase drug permeability, and make the drug contained in the patch layer better absorbed by the skin, and massage particles of different specifications can achieve different massage effects to meet the different use needs of users; at the same time, it can delay drug release, thereby prolonging the drug action time and maintaining the local effective drug concentration; provide a basis for cell attachment and proliferation, and help repair and regenerate damaged muscle tissue; the massage particles have antibacterial and wound healing effects, can prevent skin infections that may occur during the use of the patch, and are conducive to the recovery of muscle damage; improve the adhesive effect of the patch, and also reduce irritation to the skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A comparison chart of the metabolic activity test of Staphylococcus aureus provided by the present invention;

[0021] Figure 2 A comparison chart of the metabolic activity test of Escherichia coli provided by the present invention;

[0022] Figure 3 An exploded view of the Kinesio patch with large-particle massage particles of the present invention;

[0023] Figure 4An exploded view of the Kinesio patch of small-particle massage particles of the present invention;

[0024] Figure 5 An exploded view of the Kinesiology patch of the present invention having a mixture of large and small particles of massage particles.

[0025] In the figure, 1-patching layer, 2-adhesive layer, 3-particle attachment layer, 4-isolating layer, 5-massage particles, 6-release paper. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] See also Figure 1-Figure 5 , the present invention provides the following three technical solutions:

[0028] Example 1

[0029] A kinesio patch with massage particles, comprising a patch layer 1, an adhesive layer 2, a particle attachment layer 3 and an isolation layer 4 from top to bottom, a plurality of massage particles 5 are arranged on one side of the particle attachment layer 3 relative to the isolation layer 4, a plurality of air holes are arranged on the isolation layer 4, and the massage particles 5 pass through the air holes, the isolation layer 4 is adhered to the surface of the particle attachment layer 3, the adhesive layer 2 is coated on the surface of the patch layer 1, and a release paper 6 is attached to the other side during packaging, so that the patch layer 1 is packaged separately from the particle attachment layer 3 and the isolation layer 4, and the particle attachment layer 3 with massage particles 5 of different sizes is arranged in the same package;

[0030] The massage particles 5 are made of gel material, and the massage particles 5 of different specifications are distributed on the same particle attachment layer 3 in three forms: the same size, the same size, and mixed sizes. The specific preparation process is as follows:

[0031] (1) Preparation of modified hydroxyapatite: 90 mL of Tris-hydrochloric acid buffer was mixed with 10 mL of anhydrous ethanol, 2 g of hydroxyapatite was added, and ultrasonic treatment was performed for 35 minutes. Then, 0.8 g of a 10 mg / mL polydopamine solution was added, and the mixture was stirred in a dark place for 24 hours. Finally, the modified hydroxyapatite was obtained by freeze-drying.

[0032] (2) Preparation of modified chitosan: 2.5 g of chitosan was dissolved in 4 ml of 2% acetic acid solution, 4 ml of 20% hydrochloric acid solution was added dropwise, and deoxygenation treatment was performed under nitrogen atmosphere for 20 minutes. Then, 3.5 g of gallic acid was added and completely dissolved to obtain solution 1; 2.8 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide and 1.6 g of N-hydroxysuccinimide were dissolved in deionized water to obtain solution 2; under nitrogen atmosphere, solution 1 and solution 2 were mixed, dialyzed for 24 hours using a dialysis bag with a molecular weight cutoff of 3.5 kDa, and then freeze-dried to obtain modified chitosan;

[0033] (3) Preparation of massage particles 5: Mix 1 g of hyaluronidase with 120 mL of deionized water, and add 0.5 g of vitamin C as an antioxidant to prevent the enzyme from being inactivated in subsequent steps; after stirring evenly, place in a 37°C constant temperature water bath for preheating for 15 minutes; add 1 g of sodium periodate to the above solution, and stir at 200 rpm for 3 hours using a magnetic stirrer under conditions of complete light avoidance; after the reaction is completed, add 5 mL of 10% sodium thiosulfate solution to neutralize the remaining sodium periodate to prevent it from interfering with subsequent steps; add 4 mL of ethylene glycol and 0.2 g of ethylenediaminetetraacetic acid to chelate the existing metal ions to prevent them from affecting the gel properties, and continue stirring for 60 minutes; The liquid is placed in deionized water and dialyzed using a dialysis bag with a molecular weight cutoff of 10 kDa. The dialysis time is extended to 24 hours to ensure that small molecule impurities are fully removed, and then freeze-dried; 1 g of modified hydroxyapatite and 1.2 g of modified chitosan are added to the dried hyaluronidase-ethylene glycol mixture and ultrasonically stirred for 30 minutes until completely dissolved; then, stirring is continued for 30 minutes under ultrasonic stirring to form a uniform gel; the gel is injected into a mold and subjected to a freeze-thaw cycle to enhance the strength and stability of the gel. The freeze-thaw cycle is first frozen to -20°C, kept for 2 hours, and then thawed to room temperature, and repeated 3 times; finally, cutting and shaping are performed as needed to obtain the final massage particles 5.

[0034] Example 2

[0035] A kinesio patch with massage particles, comprising a patch layer 1, an adhesive layer 2, a particle attachment layer 3 and an isolation layer 4 from top to bottom, a plurality of massage particles 5 are arranged on one side of the particle attachment layer 3 relative to the isolation layer 4, a plurality of air holes are arranged on the isolation layer 4, and the massage particles 5 pass through the air holes, the isolation layer 4 is adhered to the surface of the particle attachment layer 3, the adhesive layer 2 is coated on the surface of the patch layer 1, and a release paper 6 is attached to the other side during packaging, so that the patch layer 1 is packaged separately from the particle attachment layer 3 and the isolation layer 4, and the particle attachment layer 3 with massage particles 5 of different sizes is arranged in the same package;

[0036] The massage particles 5 are made of gel material, and the massage particles 5 of different specifications are distributed on the same particle attachment layer 3 in three forms: the same size, the same size, and mixed sizes. The specific preparation process is as follows:

[0037] (1) Preparation of modified hydroxyapatite: 95 mL of Tris-hydrochloric acid buffer was mixed with 15 mL of anhydrous ethanol, 2.5 g of hydroxyapatite was added, and ultrasonic treatment was performed for 35 minutes. Then, 1.2 g of a 10 mg / mL polydopamine solution was added, and the mixture was stirred in a dark place for 24 hours. Finally, the modified hydroxyapatite was obtained by freeze-drying.

[0038] (2) Preparation of modified chitosan: 3.5 g of chitosan was dissolved in 4 ml of 2% acetic acid solution, 5 mL of 20% hydrochloric acid solution was added dropwise, and deoxygenation treatment was performed under nitrogen atmosphere for 20 minutes. Then, 5 g of gallic acid was added and completely dissolved to obtain solution 1; 3.5 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide and 2 g of N-hydroxysuccinimide were dissolved in deionized water to obtain solution 2; under nitrogen atmosphere, solution 1 and solution 2 were mixed, dialyzed for 24 hours using a dialysis bag with a molecular weight cutoff of 3.5 kDa, and then freeze-dried to obtain modified chitosan;

[0039] (3) Preparation of massage particles 5: Mix 2 g of hyaluronidase with 120 mL of deionized water, and add 2.5 g of vitamin C as an antioxidant to prevent the enzyme from being inactivated in subsequent steps; after stirring evenly, place in a 37°C constant temperature water bath for preheating for 15 minutes; add 1.5 g of sodium periodate to the above solution, and stir at 200 rpm for 3 hours using a magnetic stirrer under conditions of complete light avoidance; after the reaction is completed, add 5 mL of 10% sodium thiosulfate solution to neutralize the remaining sodium periodate to prevent it from interfering with subsequent steps; add 4.5 mL of ethylene glycol and 0.2 g of ethylenediaminetetraacetic acid to chelate the existing metal ions to prevent them from affecting the gel properties, and continue stirring for 60 minutes; The reaction solution was placed in deionized water and dialyzed using a dialysis bag with a molecular weight cutoff of 10 kDa. The dialysis time was extended to 24 hours to ensure that small molecule impurities were fully removed, and then freeze-dried. 1.2 g of modified hydroxyapatite and 0.8 g of modified chitosan were added to the dried hyaluronidase-ethylene glycol mixture and ultrasonically stirred for 30 minutes until completely dissolved. Then, stirring was continued for 30 minutes under ultrasonic stirring to form a uniform gel. The gel was injected into a mold and subjected to a freeze-thaw cycle to enhance the strength and stability of the gel. The freeze-thaw cycle was first frozen to -20°C, kept for 2 hours, and then thawed to room temperature, and repeated 3 times. Finally, cutting and shaping were performed as needed to obtain the final massage particles 5.

[0040] Example 3

[0041] A kinesio patch with massage particles, comprising a patch layer 1, an adhesive layer 2, a particle attachment layer 3 and an isolation layer 4 from top to bottom, a plurality of massage particles 5 are arranged on one side of the particle attachment layer 3 relative to the isolation layer 4, a plurality of air holes are arranged on the isolation layer 4, and the massage particles 5 pass through the air holes, the isolation layer 4 is adhered to the surface of the particle attachment layer 3, the adhesive layer 2 is coated on the surface of the patch layer 1, and a release paper 6 is attached to the other side during packaging, so that the patch layer 1 is packaged separately from the particle attachment layer 3 and the isolation layer 4, and the particle attachment layer 3 with massage particles 5 of different sizes is arranged in the same package;

[0042] The massage particles 5 are made of gel material, and the massage particles 5 of different specifications are distributed on the same particle attachment layer 3 in three forms: the same size, the same size, and mixed sizes. The specific preparation process is as follows:

[0043] (1) Preparation of modified hydroxyapatite: 100 mL of Tris-hydrochloric acid buffer was mixed with 20 mL of anhydrous ethanol, 3.5 g of hydroxyapatite was added, and ultrasonic treatment was performed for 35 minutes. Then, 1.5 g of a 10 mg / mL polydopamine solution was added, and the mixture was stirred in a dark environment for 24 hours. Finally, the modified hydroxyapatite was obtained by freeze-drying.

[0044] (2) Preparation of modified chitosan: 5 g of chitosan was dissolved in 4 ml of 2% acetic acid solution, 6 mL of 20% hydrochloric acid solution was added dropwise, and deoxygenation was carried out under nitrogen atmosphere for 20 minutes. Then, 6 g of gallic acid was added and completely dissolved to obtain solution 1; 4.2 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide and 2.5 g of N-hydroxysuccinimide were dissolved in deionized water to obtain solution 2; under nitrogen atmosphere, solution 1 and solution 2 were mixed, dialyzed for 24 hours using a dialysis bag with a molecular weight cutoff of 3.5 kDa, and then freeze-dried to obtain modified chitosan;

[0045] (3) Preparation of massage particles 5: Mix 3 g of hyaluronidase with 120 mL of deionized water, and add 2.5 g of vitamin C as an antioxidant to prevent the enzyme from being inactivated in subsequent steps; after stirring evenly, place in a 37°C constant temperature water bath for preheating for 15 minutes; add 2.5 g of sodium periodate to the above solution, and stir at 200 rpm for 3 hours using a magnetic stirrer under conditions of complete light avoidance; after the reaction is completed, add 5 mL of 10% sodium thiosulfate solution to neutralize the remaining sodium periodate to prevent it from interfering with subsequent steps; add 5 mL of ethylene glycol and 0.2 g of ethylenediaminetetraacetic acid to chelate the existing metal ions to prevent them from affecting the gel properties, and continue stirring for 60 minutes; The liquid is placed in deionized water and dialyzed using a dialysis bag with a molecular weight cutoff of 10 kDa. The dialysis time is extended to 24 hours to ensure that small molecule impurities are fully removed, and then freeze-dried; 1.2 g of modified hydroxyapatite and 0.8 g of modified chitosan are added to the dried hyaluronidase-ethylene glycol mixture and ultrasonically stirred for 30 minutes until completely dissolved; then, stirring is continued for 30 minutes under ultrasonic stirring to form a uniform gel; the gel is injected into a mold and subjected to a freeze-thaw cycle to enhance the strength and stability of the gel. The freeze-thaw cycle is first frozen to -20°C, kept for 2 hours, and then thawed to room temperature, and repeated 3 times; finally, cutting and shaping are performed as needed to obtain the final massage particles 5.

[0046] Comparative Example 1

[0047] Compared with Example 1, the difference is that the modified chitosan is replaced by chitosan; the rest remains unchanged.

[0048] Comparative Example 2

[0049] Compared with Example 1, the difference is that the modified hydroxyapatite is replaced by hydroxyapatite; the rest remains unchanged.

[0050] Escherichia coli (Gram-negative bacteria, E. coli) and Staphylococcus aureus (Gram-positive bacteria S. aureus) were used as bacterial models to detect their metabolic activities.

[0051] like Figure 1 and Figure 2 As shown, the antibacterial properties of the Kinesio patches prepared in Examples 1-3 are significantly improved compared to those in Comparative Examples 1-2.

[0052] In this process, hyaluronidase can degrade hyaluronic acid, thereby increasing the permeability and water retention capacity of tissues; vitamin C, as an antioxidant, can prevent hyaluronidase from being inactivated due to oxidation during the preparation process, ensuring the smooth progress of the enzymatic reaction;

[0053] Sodium periodate can oxidize the hydroxyl groups in the hyaluronic acid molecules to form aldehyde groups, thereby promoting the cross-linking reaction; ethylene glycol, as a reducing agent, can reduce the aldehyde groups produced by the oxidation of sodium periodate to form a stable cross-linking structure and enhance the strength of the gel;

[0054] Ethylenediaminetetraacetic acid (EDTA) is a strong metal ion chelator that can chelate metal ions in the reaction system to prevent them from interfering with the formation and performance of the gel;

[0055] Modified hydroxyapatite has excellent biocompatibility and bone bonding ability, which can enhance the biological activity of the gel; while modified chitosan has good film-forming and antibacterial properties, which can further improve the stability and biosafety of the gel; in the preparation of modified hydroxyapatite, polydopamine is used as a surface modifier to improve the surface properties of hydroxyapatite, making it easier to combine with other components while improving biocompatibility.

[0056] By optimizing the cross-linking reaction conditions and introducing stabilizers such as EDTA, the gel prepared by the present preparation process has higher stability and can maintain its structure and performance for a longer period of time; and the introduction of modified hydroxyapatite and modified chitosan, as well as the surface modification of polydopamine, make the gel prepared by the present preparation process have better biocompatibility, reduce irritation and inflammatory response to tissues during use, and have higher strength and elasticity, can better adapt to the massage needs of different parts, and improve the massage effect; because the stability and biocompatibility of the gel are improved, the massage particles prepared by the present preparation process are not easy to deteriorate and fail during use, thereby extending the service life.

[0057] This process makes the preparation process more efficient and controllable by introducing auxiliary ingredients such as antioxidants and metal ion chelators, as well as optimizing dialysis and drying conditions.

[0058] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0059] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0060] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A kinesio patch with massage particles, characterized in that: From top to bottom, it includes a patch layer, an adhesive layer, a particle attachment layer and an isolation layer. A plurality of massage particles are arranged on the particle attachment layer on one side relative to the isolation layer. A plurality of air holes are arranged on the isolation layer, and the massage particles pass through the air holes. The isolation layer is bonded to the surface of the particle attachment layer. The adhesive layer is coated on the surface of the patch layer, and release paper is attached to the other side during packaging, so that the patch layer, the particle attachment layer and the isolation layer are separated and packaged. A particle attachment layer with massage particles of different sizes is provided in the same package.

2. The kinesiology patch with massage particles according to claim 1, characterized in that: The massage particles are made of gel material, and massage particles of different specifications are distributed on the same particle attachment layer in three forms: the same size, the same size, and mixed sizes.

3. The kinesiology patch with massage particles according to claim 2, characterized in that: The specific preparation process of the massage particles is: S1. Mix 1.5-2.5 g of hyaluronidase with 120 mL of deionized water, and add 0.5 g of vitamin C as an antioxidant to prevent the enzyme from being inactivated in subsequent steps; after stirring evenly, preheat in a 37°C constant temperature water bath for 15 minutes; S2. Add 1.5-2 g of sodium periodate to the above solution, and stir at 200 rpm for 3 hours using a magnetic stirrer under conditions of complete light avoidance; after the reaction is completed, add 5 mL of 10% sodium thiosulfate solution to neutralize the remaining sodium periodate; S3, add 4-5mL of ethylene glycol and 0.2g of ethylenediaminetetraacetic acid to chelate the metal ions present, and continue stirring for 60 minutes; S4, placing the reaction solution in deionized water, dialyzing using a dialysis bag with a molecular weight cutoff of 10 kDa, extending the dialysis time to 24 hours to ensure that small molecule impurities are fully removed, and then freeze-drying; S5, adding 1-1.2 g of modified hydroxyapatite and 0.8-1.2 g of modified chitosan to the dried hyaluronidase-ethylene glycol mixture, and stirring for 30 minutes under ultrasonic stirring until completely dissolved; then stirring for another 30 minutes under ultrasonic stirring to form a uniform gel; S6. Inject the gel into the mold and perform a freeze-thaw cycle to enhance the strength and stability of the gel. The freeze-thaw cycle is to first freeze to -20°C, keep it for 2 hours, then thaw it to room temperature, and repeat 3 times; finally, cut and shape it as needed to obtain the final massage particles.

4. The kinesiology patch with massage particles according to claim 3, characterized in that: In S5, the preparation method of modified hydroxyapatite is: mixing Tris-hydrochloric acid buffer with anhydrous ethanol, adding hydroxyapatite, ultrasonic treatment for 35 minutes, then adding polydopamine solution, stirring for 24 hours in a dark environment, and finally freeze-drying to obtain modified hydroxyapatite.

5. The kinesiology patch with massage particles according to claim 4, characterized in that: During the preparation of the modified hydroxyapatite, the added amounts of the components are: 90-100 mL of Tris-hydrochloric acid buffer, 10-20 mL of ethanol, 2-3.5 g of hydroxyapatite, and 0.8-1.5 g of polydopamine, and the polydopamine solution is pre-dissolved in 10 mL of deionized water at a concentration of 10 mg / mL.

6. The kinesiology patch with massage particles according to claim 3, characterized in that: In the S5, the preparation method of modified chitosan is: dissolving chitosan in an acetic acid solution, adding a hydrochloric acid solution dropwise, deoxygenating for 20 minutes under a nitrogen atmosphere, and then adding gallic acid to obtain a solution 1 after complete dissolution; dissolving 1-ethyl-(3-dimethylaminopropyl)carbodiimide and N-hydroxysuccinimide in deionized water to obtain a solution 2; under a nitrogen atmosphere, mixing solution 1 and solution 2, dialyzing for 24 hours using a dialysis bag with a molecular weight cutoff of 3.5 kDa, and then freeze-drying to obtain the modified chitosan.

7. The kinesiology patch with massage particles according to claim 6, characterized in that: During the preparation of the modified chitosan, the added amounts of the components are: 2.5-5 g of chitosan; 4-7 ml of acetic acid solution with a concentration of 2%; 75-100 mL of deionized water; 4-6 mL of hydrochloric acid solution with a concentration of 20%; 3.5-6 g of gallic acid; 2.8-4.2 g of 1-ethyl-(3-dimethylaminopropyl)carbodiimide; and 1.6-2.5 g of N-hydroxysuccinimide.

8. A method for preparing the kinesio patch with massage particles according to any one of claims 1 to 7, characterized in that: The specific preparation method is: the patch layer and the particle attachment layer are bonded by an adhesive layer, so that the kinesio patch with massage particles is prepared.

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