Amino acid-containing compound fertilizer as well as preparation method and application thereof

Abstract

The invention discloses an amino acid-containing compound fertilizer and a preparation method and application thereof, and belongs to the technical field of fertilizers. The amino acid-containing compound fertilizer comprises the following raw materials: urea, diammonium phosphate, potassium sulfate, lysine, sweet potato stem and leaf extract, wax gourd stem and leaf extract, citric acid, borax, zinc sulfate, ammonium molybdate and humic acid. According to the amino acid-containing compound fertilizer disclosed by the invention, the inorganic fertilizer, the trace elements, the lysine, the citric acid, the plant extract and the humic acid are compounded, so that sufficient nutrients can be provided for crop growth; during preparation, the sweet potato stem and leaf extract and the wax gourd stem and leaf extract are simultaneously added in the chelation reaction process, the sweet potato stem and leaf extract and the wax gourd stem and leaf extract can cooperate with citric acid to improve the chelation effect of lysine, and meanwhile flavonoid, phenolic acids, polysaccharide and some plant growth regulating substances contained in the extract can stimulate root growth; stress resistance such as oxidation resistance and drought resistance of plants is improved, and the quality and yield of crops are remarkably improved.

Description

Technical Field

[0001] The invention belongs to the technical field of fertilizers, and in particular relates to an amino acid-containing compound fertilizer and a preparation method and application thereof. Background Art

[0002] Amino acids are indispensable nutrients in the process of plant growth and development. They are the basic units that constitute proteins in plants and also have the function of regulating plant growth and development. Plants absorb amino acids from the soil through their roots or obtain the required amino acids through endogenous synthesis, which are used in metabolic activities, cell division and growth. Amino acids are effective in promoting plant root growth, enhancing leaf photosynthesis, improving their adaptability under stress and increasing crop yields. They are one of the nutrients necessary to maintain plant growth and development. However, due to the poor stability of amino acids themselves, they are prone to decomposition under high temperature and high humidity conditions. At the same time, they are also prone to complex reactions with other components in fertilizers, especially metal ions, resulting in the consumption of the effective ingredients of amino acids, affecting their function in fertilizers. Therefore, it is particularly important to provide a compound fertilizer containing amino acids that can significantly improve the stability of amino acids and enable them to fully exert their corresponding growth-promoting functions. Summary of the invention

[0003] In view of this, the object of the present invention is to provide an amino acid-containing compound fertilizer, which can improve the stability of amino acids, promote crop growth, and improve crop stress resistance, thereby improving crop quality and yield.

[0004] Another object of the present invention is to provide a method for preparing the amino acid-containing compound fertilizer.

[0005] Another object of the present invention is to provide an application of the amino acid-containing compound fertilizer in promoting crop growth, improving crop stress resistance or increasing crop yield.

[0006] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0007] The invention provides an amino acid-containing compound fertilizer, which comprises the following raw materials: urea, diammonium phosphate, potassium sulfate, lysine, sweet potato stem and leaf extract, wax gourd stem and leaf extract, citric acid, borax, zinc sulfate, ammonium molybdate and humic acid.

[0008] Preferably, the fertilizer comprises the following raw materials in parts by weight: 15-20 parts of urea, 15-20 parts of diammonium phosphate, 10-15 parts of potassium sulfate, 2-5 parts of lysine, 1-3 parts of sweet potato stem and leaf extract, 1-3 parts of wax gourd stem and leaf extract, 1-3 parts of citric acid, 0.5-1.5 parts of borax, 0.3-1 part of zinc sulfate, 0.1-0.5 parts of ammonium molybdate and 3-8 parts of humic acid.

[0009] Preferably, the preparation method of the sweet potato stem and leaf extract comprises the following steps: washing and cutting the sweet potato stem and leaves; adding ethanol solution and ultrasonic extraction; filtering, concentrating and drying the extract to obtain the sweet potato stem and leaf extract.

[0010] Preferably, the volume fraction of ethanol in the ethanol solution is 70% to 80%.

[0011] Preferably, the preparation method of the wax gourd stem and leaf extract comprises the following steps: washing and cutting the wax gourd stem and leaf; adding ethanol solution and ultrasonic extraction; filtering, concentrating and drying the extract to obtain the wax gourd stem and leaf extract.

[0012] Preferably, the volume fraction of ethanol in the ethanol solution is 50% to 60%.

[0013] The present invention also provides a method for preparing the amino acid-containing compound fertilizer, the method comprising the following steps:

[0014] Weigh the raw materials by weight; dissolve the lysine and the citric acid in water respectively to obtain a lysine aqueous solution and a citric acid aqueous solution, mix and dissolve the sweet potato stem and leaf extract and the wax gourd stem and leaf extract in water to obtain a sweet potato and wax gourd stem and leaf extract suspension; add the lysine aqueous solution to the citric acid aqueous solution and stir, add the sweet potato and wax gourd stem and leaf extract suspension during the stirring process, carry out a chelating reaction, and obtain a reaction solution; add urea, diammonium phosphate, potassium sulfate, borax, zinc sulfate, ammonium molybdate and humic acid to the reaction solution in sequence and stir; granulate and dry after the stirring is completed to obtain an amino acid-containing compound fertilizer.

[0015] Preferably, the mass fraction of lysine in the lysine aqueous solution is 30% to 50%; and / or the mass fraction of citric acid in the citric acid aqueous solution is 20% to 40%; and / or the mass fraction of sweet potato stem and leaf extract in the sweet potato and wax gourd stem and leaf extract suspension is 5% to 10%, and the mass fraction of wax gourd stem and leaf extract is 5% to 10%.

[0016] Preferably, the chelating reaction is carried out at a temperature of 40 to 60° C. and for a time of 2 to 4 hours.

[0017] The present invention also provides an application of the amino acid-containing compound fertilizer in promoting crop growth, improving crop stress resistance or increasing crop yield.

[0018] Beneficial effects of the present invention:

[0019] The amino acid-containing compound fertilizer of the present invention can provide sufficient nutrients for crop growth by compounding inorganic fertilizer, trace elements, lysine, citric acid, plant extract and humic acid; during preparation, sweet potato stem and leaf extract and wax gourd stem and leaf extract are added simultaneously during the chelation reaction process, and the two can synergistically improve the lysine chelation effect with citric acid, prompting lysine to form a more stable chelate structure, so that the stability of lysine in the fertilizer is further improved, and at the same time, the flavonoids, phenolic acids, polysaccharides and some plant growth regulators contained therein can stimulate root growth and improve plant antioxidant, drought resistance and other stress resistance. The amino acid-containing compound fertilizer of the present invention is used as base fertilizer and topdressing for Chinese cabbage planting. Compared with commercially available chemical fertilizers, the application of the amino acid-containing compound fertilizer of the present invention increases plant height by 32.25%, the number of leaves by 21.01%, the weight per plant by 15.25%, and the yield of the plot by 48.83%, significantly improving the quality and yield of Chinese cabbage. DETAILED DESCRIPTION

[0020] The invention provides an amino acid-containing compound fertilizer, which comprises the following raw materials: urea, diammonium phosphate, potassium sulfate, lysine, sweet potato stem and leaf extract, wax gourd stem and leaf extract, citric acid, borax, zinc sulfate, ammonium molybdate and humic acid.

[0021] In the present invention, the fertilizer preferably includes the following raw materials in parts by weight: 15-20 parts of urea, 15-20 parts of diammonium phosphate, 10-15 parts of potassium sulfate, 2-5 parts of lysine, 1-3 parts of sweet potato stem and leaf extract, 1-3 parts of wax gourd stem and leaf extract, 1-3 parts of citric acid, 0.5-1.5 parts of borax, 0.3-1 part of zinc sulfate, 0.1-0.5 parts of ammonium molybdate and 3-8 parts of humic acid. The weight portion of urea is further preferably 17-19 parts, and more preferably 18 parts; the weight portion of diammonium phosphate is further preferably 16-18 parts, and more preferably 17 parts; the weight portion of potassium sulfate is further preferably 12-14 parts, and more preferably 13 parts; the weight portion of lysine is further preferably 2.5-4 parts, and more preferably 3 parts; the weight portion of sweet potato stem and leaf extract is further preferably 1.5-2.5 parts, and more preferably 2 parts; the weight portion of wax gourd stem and leaf extract is further preferably 1.5-2.5 parts, and more preferably 2 parts; the weight portion of citric acid is further preferably 1.5-2.5 parts, and more preferably 2 parts; the weight portion of borax is further preferably 0.8-1.2 parts, and more preferably 1 part; the weight portion of zinc sulfate is further preferably 0.4-0.8 parts, and more preferably 0.6 parts; the weight portion of ammonium molybdate is further preferably 0.2-0.4 parts, and more preferably 0.3 parts; the weight portion of humic acid is further preferably 4-6 parts, and more preferably 5 parts.

[0022] In the present invention, there is no particular limitation on the sources of the urea, diammonium phosphate, potassium sulfate, lysine, citric acid, borax, zinc sulfate, ammonium molybdate and humic acid, and they can be purchased through conventional channels.

[0023] In the present invention, the sweet potato stem and leaf extract is preferably an alcohol extract of sweet potato stems and leaves; as an optional embodiment, the preparation method of the sweet potato stem and leaf extract preferably includes the following steps: washing and cutting the sweet potato stems and leaves; adding ethanol solution and ultrasonic extraction; filtering, concentrating and drying the extract to obtain the sweet potato stem and leaf extract. The volume fraction of ethanol in the ethanol solution is preferably 70% to 80%, further preferably 72% to 78%, and more preferably 75%; the mass volume ratio of the sweet potato stems and leaves to the ethanol solution is preferably 1g:5 to 10mL, further preferably 1g:6 to 9mL, and more preferably 1g:8mL; the ultrasonic frequency is preferably 20 to 30kHz, further preferably 22 to 28kHz, and more preferably 25kHz; the ultrasonic time is preferably 30 to 40min, further preferably 32 to 38min, and more preferably 35min; the number of extractions can be conventionally selected according to actual needs, preferably 1 to 3 times, and more preferably 2 times; after the extraction is completed, the extracts are combined; the methods of filtering, concentrating and drying the extracts can be conventionally selected according to actual needs; after drying, the sweet potato stem and leaf powder extract is obtained.

[0024] In the present invention, the wax gourd stem and leaf extract is preferably an alcohol extract of wax gourd stem and leaves; as an optional embodiment, the preparation method of the wax gourd stem and leaf extract preferably includes the following steps: washing and cutting the wax gourd stem and leaves; adding ethanol solution and ultrasonic extraction; filtering, concentrating and drying the extract to obtain the wax gourd stem and leaf extract. The volume fraction of ethanol in the ethanol solution is preferably 50% to 60%, further preferably 52% to 58%, and more preferably 55%; the mass volume ratio of the wax gourd stems and leaves to the ethanol solution is preferably 1g:8 to 12mL, further preferably 1g:9 to 11mL, and more preferably 1g:10mL; the ultrasonic frequency is preferably 20 to 30kHz, further preferably 22 to 28kHz, and more preferably 25kHz; the ultrasonic time is preferably 30 to 40min, further preferably 32 to 38min, and more preferably 35min; the number of extractions can be conventionally selected according to actual needs, preferably 1 to 3 times, and more preferably 2 times; after the extraction is completed, the extracts are combined; the methods of filtering, concentrating, and drying the extracts can be conventionally selected according to actual needs; after drying, the wax gourd stem and leaf powder extract is obtained.

[0025] The present invention also provides a method for preparing the amino acid-containing compound fertilizer, and the preparation method preferably comprises the following steps:

[0026] Weigh the raw materials by weight; dissolve the lysine and the citric acid in water respectively to obtain a lysine aqueous solution and a citric acid aqueous solution, mix and dissolve the sweet potato stem and leaf extract and the wax gourd stem and leaf extract in water to obtain a sweet potato and wax gourd stem and leaf extract suspension; add the lysine aqueous solution to the citric acid aqueous solution and stir, add the sweet potato and wax gourd stem and leaf extract suspension during the stirring process, carry out a chelating reaction, and obtain a reaction solution; add urea, diammonium phosphate, potassium sulfate, borax, zinc sulfate, ammonium molybdate and humic acid to the reaction solution in sequence and stir; granulate and dry after the stirring is completed to obtain an amino acid-containing compound fertilizer.

[0027] In the present invention, the mass fraction of lysine in the lysine aqueous solution is preferably 30% to 50%, further preferably 35% to 45%, and more preferably 40%; the mass fraction of citric acid in the citric acid aqueous solution is preferably 20% to 40%, further preferably 25% to 35%, and more preferably 30%; the mass fraction of sweet potato stem and leaf extract in the sweet potato and wax gourd stem and leaf extract suspension is preferably 5% to 10%, further preferably 6% to 8%, and more preferably 7.5%; the mass fraction of wax gourd stem and leaf extract in the sweet potato and wax gourd stem and leaf extract suspension is preferably 5% to 10%, further preferably 6% to 8%, and more preferably 7.5%.

[0028] In the present invention, the lysine aqueous solution is added to the citric acid aqueous solution and stirred; the stirring speed is preferably 200-300 r / min, more preferably 225-275 r / min, and more preferably 250 r / min; the sweet potato and wax gourd stem and leaf extract suspension is added during the stirring process to carry out a chelation reaction; the chelation reaction temperature is preferably 40-60°C, more preferably 45-55°C, and more preferably 50°C; the chelation reaction pH is preferably 4-6, more preferably 4.5-5.5, and more preferably 5; the chelation reaction time is preferably 2-4h, more preferably 2.5-3.5h, and more preferably 3h.

[0029] In the present invention, sweet potato stem and leaf extract and wax gourd stem and leaf extract are added during the lysine chelation reaction process. The flavonoids, phenolic acids and other components contained in these extracts can produce a synergistic effect with citric acid. The phenolic hydroxyl groups and other groups contained in them can work together with the amino group of lysine and citric acid to form a more stable chelate structure, so that the stability of lysine in the fertilizer is further improved, and the chelation effect of lysine is enhanced.

[0030] In the present invention, after the chelating reaction is completed, urea, diammonium phosphate, potassium sulfate, borax, zinc sulfate, ammonium molybdate and humic acid are added to the reaction solution in sequence, and stirred to mix the raw materials evenly. The stirring speed and time can be conventionally selected according to actual needs. As an optional embodiment, the stirring speed is preferably 150 to 250 r / min, more preferably 175 to 225 r / min, and more preferably 200 r / min; the stirring time is preferably 20 to 30 min, more preferably 22 to 28 min, and more preferably 25 min.

[0031] In the present invention, the material after stirring is granulated and dried to obtain the amino acid-containing compound fertilizer. The granulation and drying methods can be conventionally selected according to actual needs.

[0032] The present invention also provides an application of the compound fertilizer in promoting crop growth, improving crop stress resistance or increasing crop yield.

[0033] In the present invention, urea, diammonium phosphate and potassium sulfate in the amino acid-containing compound fertilizer can provide sufficient nitrogen, phosphorus and potassium, which are basic nutrients necessary for plant growth; the compound fertilizer also contains a variety of trace elements, such as boron, zinc and molybdenum, which promote the growth and development of plants and the absorption and metabolism of nutrient elements; humic acid can improve soil structure, increase soil aeration and water retention, and is beneficial to plant root growth and nutrient absorption, and at the same time, it can also improve the utilization rate of fertilizer; lysine is a basic component unit of protein in plants, can be directly absorbed and utilized by plants, and participates in a variety of physiological activities in plants, such as enzyme synthesis of photosynthesis and respiration, and adding lysine to the compound fertilizer in a chelating manner can improve the stability of lysine; sweet potato stem and leaf extract and wax gourd stem and leaf extract can not only synergistically improve the lysine chelating effect with citric acid, but also the flavonoids, phenolic acids, polysaccharides and some plant growth regulators contained therein can stimulate root growth and improve the plant's antioxidant, drought resistance and other stress resistance properties. The present invention can provide sufficient nutrients for crop growth, promote crop growth, improve stress resistance, and thus increase crop yield by compounding inorganic fertilizers, trace elements, lysine, citric acid, plant extracts and humic acid.

[0034] In the present invention, the amino acid-containing compound fertilizer can be used as a base fertilizer or as a topdressing fertilizer. Since it contains fertilizer components such as urea, diammonium phosphate, potassium sulfate, etc., it can provide necessary macronutrients for the initial stage of crop growth, trace elements such as borax, zinc sulfate, ammonium molybdate, etc. can also lay a nutrient foundation for the entire growth period of crops, lysine can be directly absorbed and utilized by plants, and participates in various physiological activities in plants, and organic components such as humic acid can improve soil structure and increase soil water and fertilizer retention capacity, so it can be used as a base fertilizer. In the process of crop growth, as crops consume nutrients, the nutrients in the soil gradually decrease, and the quick-acting nutrients (such as fertilizer components such as urea) in the amino acid-containing compound fertilizer of the present invention can timely supplement the elements such as nitrogen, phosphorus, and potassium required by crops, meet the demand for nutrients in the later stage of crop growth, and the components such as amino acids (lysine) and plant extracts can play a role in regulating growth and enhancing stress resistance during the critical period of crop growth, so it can also be used as a topdressing fertilizer. Specific fertilization methods and application amounts can be conventionally selected according to actual needs. For example, when applied as a base fertilizer for leafy vegetables, the application amount of the amino acid-containing compound fertilizer is preferably 20 to 60 kg / mu, more preferably 30 to 50 kg / mu, and more preferably 40 kg / mu. When applied as a topdressing fertilizer for leafy vegetables, the number of topdressing of the amino acid-containing compound fertilizer is preferably 1 to 3 times, more preferably 2 times; the amount of topdressing each time is preferably 10 to 20 kg / mu, more preferably 12 to 18 kg / mu, and more preferably 15 kg / mu. There is no special limitation on the types of crops to which the amino acid-containing compound fertilizer of the present invention is applied, and it can be conventionally selected according to actual needs.

[0035] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0036] In the following embodiments, unless otherwise specified, all of them are conventional methods.

[0037] Unless otherwise specified, the materials and reagents used in the following examples can be obtained from commercial sources.

[0038] Example 1

[0039] A compound fertilizer containing amino acids, wherein the fertilizer raw materials are as follows in parts by weight: 18 parts of urea, 17 parts of diammonium phosphate, 13 parts of potassium sulfate, 3 parts of lysine, 2 parts of sweet potato stem and leaf extract, 2 parts of wax gourd stem and leaf extract, 2 parts of citric acid, 1 part of borax, 0.6 parts of zinc sulfate, 0.3 parts of ammonium molybdate and 5 parts of humic acid.

[0040] The preparation method of the sweet potato stem and leaf extract is as follows: wash and cut the sweet potato stem and leaves; add an ethanol solution with a volume fraction of 75%, and the volume ratio of the sweet potato stem and leaves to the ethanol solution is 1g:8mL; ultrasonic extraction, the ultrasonic frequency is 25kHz, and the extraction time is 35min; filter the extract, repeat the above operation on the filter residue to extract again, filter and combine the filtrate; concentrate and dry to obtain the sweet potato stem and leaf extract.

[0041] The preparation method of the wax gourd stem and leaf extract is as follows: wash and cut the wax gourd stem and leaves; add an ethanol solution with a volume fraction of 55%, and the volume ratio of the wax gourd stem and leaves to the ethanol solution is 1g:10mL; ultrasonic extraction, the ultrasonic frequency is 25kHz, and the extraction time is 35min; filter the extract, repeat the above operation on the filter residue to extract again, filter and combine the filtrate; concentrate and dry to obtain the wax gourd stem and leaf extract.

[0042] The preparation method of the amino acid-containing compound fertilizer comprises the following steps:

[0043] Raw material preparation: weigh each raw material by weight; dissolve the lysine and the citric acid in water respectively to obtain a lysine aqueous solution and a citric acid aqueous solution, wherein the mass fraction of lysine in the lysine aqueous solution is 40%, and the mass fraction of citric acid in the citric acid aqueous solution is 30%; mix the sweet potato stem and leaf extract and the wax gourd stem and leaf extract, and dissolve them in water to obtain a sweet potato and wax gourd stem and leaf extract suspension, wherein the mass fraction of the sweet potato stem and leaf extract and the mass fraction of the wax gourd stem and leaf extract in the sweet potato and wax gourd stem and leaf extract suspension is 7.5%, and the mass fraction of the wax gourd stem and leaf extract is 7.5%;

[0044] Chelating reaction: adding the lysine aqueous solution to the citric acid aqueous solution and stirring at a stirring speed of 250 r / min, adding the sweet potato and wax gourd stem and leaf extract suspension during the stirring process to carry out a chelating reaction, wherein the chelating reaction temperature is 50° C., the pH value is 5, and the reaction time is 3 h to obtain a reaction solution;

[0045] Raw material mixing: urea, diammonium phosphate, potassium sulfate, borax, zinc sulfate, ammonium molybdate and humic acid were added to the reaction solution in sequence, and stirred at a stirring speed of 200 r / min for 25 min;

[0046] Granulation: After stirring, granulation and drying are performed to obtain amino acid-containing compound fertilizer.

[0047] Example 2

[0048] A compound fertilizer containing amino acids, wherein the fertilizer raw materials are as follows in parts by weight: 15 parts of urea, 15 parts of diammonium phosphate, 10 parts of potassium sulfate, 2 parts of lysine, 1 part of sweet potato stem and leaf extract, 1 part of wax gourd stem and leaf extract, 1 part of citric acid, 0.5 parts of borax, 0.3 parts of zinc sulfate, 0.1 parts of ammonium molybdate and 3 parts of humic acid.

[0049] The preparation method of the sweet potato stem and leaf extract is as follows: washing and cutting the sweet potato stem and leaves; adding an ethanol solution with a volume fraction of 70%, with the volume ratio of the sweet potato stem and leaves to the ethanol solution being 1 g:10 mL; ultrasonic extraction with an ultrasonic frequency of 20 kHz and an extraction time of 40 minutes; filtering, concentrating and drying the extract to obtain the sweet potato stem and leaf extract.

[0050] The preparation method of the wax gourd stem and leaf extract is as follows: wash and cut the wax gourd stem and leaves; add an ethanol solution with a volume fraction of 50%, and the volume ratio of the wax gourd stem and leaves to the ethanol solution is 1g:12mL; ultrasonic extraction, the ultrasonic frequency is 20kHz, and the extraction time is 40min; the extract is filtered, concentrated, and dried to obtain the wax gourd stem and leaf extract.

[0051] The preparation method of the amino acid-containing compound fertilizer comprises the following steps:

[0052] Raw material preparation: weigh each raw material by weight; dissolve the lysine and the citric acid in water respectively to obtain a lysine aqueous solution and a citric acid aqueous solution, wherein the mass fraction of lysine in the lysine aqueous solution is 30%, and the mass fraction of citric acid in the citric acid aqueous solution is 20%; mix the sweet potato stem and leaf extract and the wax gourd stem and leaf extract, and dissolve them in water to obtain a sweet potato and wax gourd stem and leaf extract suspension, wherein the mass fraction of the sweet potato stem and leaf extract in the sweet potato and wax gourd stem and leaf extract suspension is 5%, and the mass fraction of the wax gourd stem and leaf extract is 5%;

[0053] Chelating reaction: adding the lysine aqueous solution to the citric acid aqueous solution and stirring at a stirring speed of 200 r / min, adding the sweet potato and wax gourd stem and leaf extract suspension during stirring to carry out a chelating reaction, wherein the chelating reaction temperature is 60° C., the pH value is 4, and the time is 2 h to obtain a reaction solution;

[0054] Raw material mixing: urea, diammonium phosphate, potassium sulfate, borax, zinc sulfate, ammonium molybdate and humic acid were added to the reaction solution in sequence, and stirred at a stirring speed of 150 r / min for 30 min;

[0055] Granulation: After stirring, granulation and drying are performed to obtain amino acid-containing compound fertilizer.

[0056] Example 3

[0057] A compound fertilizer containing amino acids, wherein the fertilizer raw materials are as follows in parts by weight: 20 parts of urea, 20 parts of diammonium phosphate, 15 parts of potassium sulfate, 5 parts of lysine, 3 parts of sweet potato stem and leaf extract, 3 parts of wax gourd stem and leaf extract, 3 parts of citric acid, 1.5 parts of borax, 1 part of zinc sulfate, 0.5 parts of ammonium molybdate and 8 parts of humic acid.

[0058] The preparation method of the sweet potato stem and leaf extract is as follows: wash and cut the sweet potato stem and leaves; add an ethanol solution with a volume fraction of 80%, and the volume ratio of the sweet potato stem and leaves to the ethanol solution is 1g:5mL; ultrasonic extraction, the ultrasonic frequency is 30kHz, and the extraction time is 30min; filter the extract, repeat the above operation twice on the filter residue, and combine the filtrate after filtering; concentrate and dry to obtain the sweet potato stem and leaf extract.

[0059] The preparation method of the wax gourd stem and leaf extract is as follows: wash and cut the wax gourd stem and leaves; add an ethanol solution with a volume fraction of 60%, and the volume ratio of the wax gourd stem and leaves to the ethanol solution is 1g:8mL; ultrasonic extraction, the ultrasonic frequency is 30kHz, and the extraction time is 30min; filter the extract, repeat the above operation twice on the filter residue, and combine the filtrate after filtering; concentrate and dry to obtain the wax gourd stem and leaf extract.

[0060] The preparation method of the amino acid-containing compound fertilizer comprises the following steps:

[0061] Raw material preparation: weigh each raw material by weight; dissolve the lysine and the citric acid in water respectively to obtain a lysine aqueous solution and a citric acid aqueous solution, wherein the mass fraction of lysine in the lysine aqueous solution is 50%, and the mass fraction of citric acid in the citric acid aqueous solution is 40%; mix the sweet potato stem and leaf extract and the wax gourd stem and leaf extract, and dissolve them in water to obtain a sweet potato and wax gourd stem and leaf extract suspension, wherein the mass fraction of the sweet potato stem and leaf extract in the sweet potato and wax gourd stem and leaf extract suspension is 10%, and the mass fraction of the wax gourd stem and leaf extract is 10%;

[0062] Chelating reaction: adding the lysine aqueous solution to the citric acid aqueous solution and stirring at a stirring speed of 300 r / min, adding the sweet potato and wax gourd stem and leaf extract suspension during the stirring process to carry out a chelating reaction, wherein the chelating reaction temperature is 40° C., the pH is 6, and the time is 4 hours to obtain a reaction solution;

[0063] Raw material mixing: urea, diammonium phosphate, potassium sulfate, borax, zinc sulfate, ammonium molybdate and humic acid were added to the reaction solution in sequence, and stirred at a stirring speed of 250 r / min for 20 min;

[0064] Granulation: After stirring, granulation and drying are performed to obtain amino acid-containing compound fertilizer.

[0065] Example 4

[0066] A compound fertilizer containing amino acids, wherein the fertilizer raw materials are as follows in parts by weight: 17 parts of urea, 16 parts of diammonium phosphate, 12 parts of potassium sulfate, 2.5 parts of lysine, 1.5 parts of sweet potato stem and leaf extract, 1.5 parts of wax gourd stem and leaf extract, 1.5 parts of citric acid, 0.8 parts of borax, 0.4 parts of zinc sulfate, 0.2 parts of ammonium molybdate and 4 parts of humic acid.

[0067] The preparation method of the sweet potato stem and leaf extract is as follows: wash and cut the sweet potato stem and leaves; add an ethanol solution with a volume fraction of 72%, and the volume ratio of the sweet potato stem and leaves to the ethanol solution is 1g:9mL; ultrasonic extraction, the ultrasonic frequency is 22kHz, and the extraction time is 38min; filter the extract, repeat the above operation on the filter residue to extract again, filter and combine the filtrate; concentrate and dry to obtain the sweet potato stem and leaf extract.

[0068] The preparation method of the wax gourd stem and leaf extract is as follows: wash and cut the wax gourd stem and leaves; add an ethanol solution with a volume fraction of 52%, and the volume ratio of the wax gourd stem and leaves to the ethanol solution is 1g:11mL; ultrasonic extraction, the ultrasonic frequency is 22kHz, and the extraction time is 38min; filter the extract, repeat the above operation on the filter residue to extract again, filter and combine the filtrate; concentrate and dry to obtain the wax gourd stem and leaf extract.

[0069] The preparation method of the amino acid-containing compound fertilizer comprises the following steps:

[0070] Raw material preparation: weigh each raw material by weight; dissolve the lysine and the citric acid in water respectively to obtain a lysine aqueous solution and a citric acid aqueous solution, wherein the mass fraction of lysine in the lysine aqueous solution is 35%, and the mass fraction of citric acid in the citric acid aqueous solution is 25%; mix the sweet potato stem and leaf extract and the wax gourd stem and leaf extract, and dissolve them in water to obtain a sweet potato and wax gourd stem and leaf extract suspension, wherein the mass fraction of the sweet potato stem and leaf extract in the sweet potato and wax gourd stem and leaf extract suspension is 6%, and the mass fraction of the wax gourd stem and leaf extract is 6%;

[0071] Chelating reaction: adding the lysine aqueous solution to the citric acid aqueous solution and stirring at a stirring speed of 225 r / min, adding the sweet potato and wax gourd stem and leaf extract suspension during stirring to carry out a chelating reaction, wherein the chelating reaction temperature is 55° C., the pH value is 4.5, and the reaction time is 2.5 h to obtain a reaction solution;

[0072] Raw material mixing: urea, diammonium phosphate, potassium sulfate, borax, zinc sulfate, ammonium molybdate and humic acid were added to the reaction solution in sequence, and stirred at a stirring speed of 175 r / min for 28 min;

[0073] Granulation: After stirring, granulation and drying are performed to obtain amino acid-containing compound fertilizer.

[0074] Example 5

[0075] A compound fertilizer containing amino acids, wherein the fertilizer raw materials are as follows in parts by weight: 19 parts of urea, 18 parts of diammonium phosphate, 14 parts of potassium sulfate, 4 parts of lysine, 2.5 parts of sweet potato stem and leaf extract, 2.5 parts of wax gourd stem and leaf extract, 2.5 parts of citric acid, 1.2 parts of borax, 0.8 parts of zinc sulfate, 0.4 parts of ammonium molybdate and 6 parts of humic acid.

[0076] The preparation method of the sweet potato stem and leaf extract is as follows: wash and cut the sweet potato stem and leaves; add an ethanol solution with a volume fraction of 78%, and the volume ratio of the sweet potato stem and leaves to the ethanol solution is 1g:6mL; ultrasonic extraction, the ultrasonic frequency is 28kHz, and the extraction time is 32min; filter the extract, repeat the above operation twice on the filter residue, and combine the filtrate after filtering; concentrate and dry to obtain the sweet potato stem and leaf extract.

[0077] The preparation method of the wax gourd stem and leaf extract is as follows: wash and cut the wax gourd stem and leaves; add an ethanol solution with a volume fraction of 58%, and the volume ratio of the wax gourd stem and leaves to the ethanol solution is 1g:9mL; ultrasonic extraction, the ultrasonic frequency is 28kHz, and the extraction time is 32min; filter the extract, repeat the above operation twice on the filter residue, and combine the filtrate after filtering; concentrate and dry to obtain the wax gourd stem and leaf extract.

[0078] The preparation method of the amino acid-containing compound fertilizer comprises the following steps:

[0079] Raw material preparation: weigh each raw material by weight; dissolve the lysine and the citric acid in water respectively to obtain a lysine aqueous solution and a citric acid aqueous solution, wherein the mass fraction of lysine in the lysine aqueous solution is 45%, and the mass fraction of citric acid in the citric acid aqueous solution is 35%; mix the sweet potato stem and leaf extract and the wax gourd stem and leaf extract, and dissolve them in water to obtain a sweet potato and wax gourd stem and leaf extract suspension, wherein the mass fraction of the sweet potato stem and leaf extract and the mass fraction of the wax gourd stem and leaf extract in the sweet potato and wax gourd stem and leaf extract suspension is 8%, and the mass fraction of the wax gourd stem and leaf extract is 8%;

[0080] Chelating reaction: adding the lysine aqueous solution to the citric acid aqueous solution and stirring at a stirring speed of 275 r / min, adding the sweet potato and wax gourd stem and leaf extract suspension during stirring to carry out a chelating reaction, wherein the chelating reaction temperature is 45° C., the pH value is 5.5, and the reaction time is 3.5 h to obtain a reaction solution;

[0081] Raw material mixing: urea, diammonium phosphate, potassium sulfate, borax, zinc sulfate, ammonium molybdate and humic acid were added to the reaction solution in sequence, and stirred at a stirring speed of 225 r / min for 22 min;

[0082] Granulation: After stirring, granulation and drying are performed to obtain amino acid-containing compound fertilizer.

[0083] Comparative Examples 1 to 3

[0084] An amino acid-containing compound fertilizer, the weight parts of each raw material are shown in Table 1:

[0085] Table 1 Composition of raw materials of amino acid-containing compound fertilizers of Comparative Examples 1 to 3

[0086]

[0087]

[0088] The preparation method of the sweet potato stem and leaf extract or wax gourd stem and leaf extract is the same as that in Example 1.

[0089] Preparation method of the amino acid-containing compound fertilizer: The preparation methods of comparative examples 1 to 3 are the same as those of example 1 except that the composition of the above-mentioned raw materials is different and the raw materials added during the chelating reaction are different, and the remaining steps are the same.

[0090] Test Example 1

[0091] The amino acid-containing compound fertilizer prepared in Example 1 and Comparative Examples 1 to 3, as well as commercially available fertilizer (15-15-15), were used to carry out a Chinese cabbage planting experiment in a vegetable greenhouse, and the Chinese cabbage variety was Jingyan Kuaicai. The experiment set up 5 treatments, including the experimental group (Example 1), the control group 1 (Comparative Example 1), the control group 2 (Comparative Example 2), the control group 3 (Comparative Example 3), and the control group 4 (commercially available fertilizer), each treatment was repeated 3 times, and a random block arrangement method was adopted. The area of ​​the plot was 2m×3m, and 100 Chinese cabbages were planted in each plot.

[0092] The experimental group and the control group applied 1 to 4 fertilizers as base fertilizers, and the application amount was converted to 40 kg / mu; the first topdressing was carried out when the pakchoi seedlings grew 3 to 4 true leaves, and the application amount was converted to 15 kg / mu; the second topdressing was carried out when the pakchoi grew to 8 to 10 true leaves and was about to enter the rosette stage, and the application amount was converted to 15 kg / mu. The rest of the management measures of each treatment group were the same.

[0093] When the Chinese cabbage was harvested, 10 plants were randomly selected from each treatment group to measure plant height, leaf number, plant weight and average yield of the plot. The results are shown in Table 2.

[0094] Table 2 Quality and yield of Chinese cabbage under different treatments

[0095] Grouping Plant height(cm) Number of blades Single plant weight (g) Plot yield (kg) Experimental Group 39.90 14.40 347.60 31.80 Control group 1 37.78 13.90 335.10 28.37 Control group 2 38.04 13.70 333.90 27.70 Control group 3 34.45 12.50 316.60 25.40 Control group 4 30.17 11.90 301.60 21.37

[0096] It can be seen from the results in Table 2 that the amino acid-containing compound fertilizer of the present invention can significantly increase the plant height, leaf number, plant weight and yield of Chinese cabbage; compared with commercially available chemical fertilizers (control group 4), the plant height increased by 32.25%, the leaf number increased by 21.01%, the plant weight increased by 15.25%, and the plot yield increased by 48.83% after the application of the amino acid-containing compound fertilizer of the present invention; compared with the use of the sweet potato stem and leaf extract (control group 1) or the wax gourd stem and leaf extract (control group 2) in the raw materials, the application of the amino acid-containing compound fertilizer of the present invention increased the plant height by 32.25%, the leaf number increased by 21.01%, the plant weight increased by 15.25%, and the plot yield increased by 48.83%. The fertilizer increased plant height by 4.89% to 5.61%, the number of leaves by 3.60% to 5.11%, the weight of a single plant by 3.73% to 4.10%, and the yield of a plot by 12.10% to 14.80%; compared with the case where both the sweet potato stem and leaf extract and the wax gourd stem and leaf extract were discarded from the raw materials (control group 3), the application of the amino acid-containing compound fertilizer of the present invention increased plant height by 15.82%, the number of leaves by 15.20%, the weight of a single plant by 9.79%, and the yield of a plot by 25.20%. It is shown that the amino acid-containing composite fertilizer of the present invention can provide sufficient nutrients for crop growth by compounding inorganic fertilizers, trace elements, lysine, citric acid, plant extracts and humic acid. At the same time, adding sweet potato stem and leaf extracts and wax gourd stem and leaf extracts during the chelating reaction can not only synergize with citric acid to improve the lysine chelating effect, but also the flavonoids, phenolic acids, polysaccharides and some plant growth regulators contained therein can stimulate root growth and improve the plant's antioxidant, drought resistance and other stress resistance properties. The components work synergistically to ultimately achieve the improvement of crop quality and yield.

[0097] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. An amino acid-containing compound fertilizer, characterized in that: The fertilizer comprises the following raw materials: urea, diammonium phosphate, potassium sulfate, lysine, sweet potato stem and leaf extract, wax gourd stem and leaf extract, citric acid, borax, zinc sulfate, ammonium molybdate and humic acid.

2. The amino acid-containing compound fertilizer according to claim 1, characterized in that: The fertilizer comprises the following raw materials in parts by weight: 15-20 parts of urea, 15-20 parts of diammonium phosphate, 10-15 parts of potassium sulfate, 2-5 parts of lysine, 1-3 parts of sweet potato stem and leaf extract, 1-3 parts of wax gourd stem and leaf extract, 1-3 parts of citric acid, 0.5-1.5 parts of borax, 0.3-1 part of zinc sulfate, 0.1-0.5 parts of ammonium molybdate and 3-8 parts of humic acid.

3. The amino acid-containing compound fertilizer according to claim 1, characterized in that: The preparation method of the sweet potato stem and leaf extract comprises the following steps: washing and cutting the sweet potato stem and leaf; adding ethanol solution and ultrasonically extracting; filtering, concentrating and drying the extract to obtain the sweet potato stem and leaf extract.

4. The amino acid-containing compound fertilizer according to claim 3, characterized in that: The volume fraction of ethanol in the ethanol solution is 70% to 80%.

5. The amino acid-containing compound fertilizer according to claim 1, characterized in that: The preparation method of the wax gourd stem and leaf extract comprises the following steps: washing and cutting the wax gourd stem and leaf; adding ethanol solution and ultrasonically extracting; filtering, concentrating and drying the extract to obtain the wax gourd stem and leaf extract.

6. The amino acid-containing compound fertilizer according to claim 5, characterized in that: The volume fraction of ethanol in the ethanol solution is 50% to 60%.

7. The method for preparing the amino acid-containing compound fertilizer according to any one of claims 1 to 6, characterized in that: The preparation method comprises the following steps: Weigh the raw materials by weight; dissolve the lysine and the citric acid in water respectively to obtain a lysine aqueous solution and a citric acid aqueous solution, mix and dissolve the sweet potato stem and leaf extract and the wax gourd stem and leaf extract in water to obtain a sweet potato and wax gourd stem and leaf extract suspension; add the lysine aqueous solution to the citric acid aqueous solution and stir, add the sweet potato and wax gourd stem and leaf extract suspension during the stirring process, carry out a chelating reaction, and obtain a reaction solution; add urea, diammonium phosphate, potassium sulfate, borax, zinc sulfate, ammonium molybdate and humic acid to the reaction solution in sequence and stir; granulate and dry after the stirring is completed to obtain an amino acid-containing compound fertilizer.

8. The preparation method according to claim 7, characterized in that: The mass fraction of lysine in the lysine aqueous solution is 30% to 50%; and / or the mass fraction of citric acid in the citric acid aqueous solution is 20% to 40%; and / or the mass fraction of sweet potato stem and leaf extract in the sweet potato and wax gourd stem and leaf extract suspension is 5% to 10%, and the mass fraction of wax gourd stem and leaf extract is 5% to 10%.

9. The preparation method according to claim 7, characterized in that: The temperature of the chelating reaction is 40-60° C. and the time is 2-4 hours.

10. Use of the amino acid-containing compound fertilizer according to any one of claims 1 to 6 in promoting crop growth, improving crop stress resistance or increasing crop yield.

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