Production process of microbial deodorant

Abstract

The invention relates to the technical field of deodorants, in particular to a production process of a microbial deodorant. The production process of the microbial deodorant comprises the following steps: stirring a composite fermentation liquid at room temperature to obtain a composite bacterial liquid; mixing and stirring the compound bacteria liquid, a microbial additive and a carrier according to the dosage ratio of 1mL: (0.05-0.2) g: (0.8-1.2) g, and drying to obtain the microbial deodorant. The microbial additive is any one of yeast cell wall extract, plant fiber powder and protein powder; the carrier is modified porous medical stone. The prepared microbial deodorant has a good deodorization effect, meanwhile, the deodorization effect has durability, and the preparation method is simple and suitable for large-scale popularization.

Description

Technical Field

[0001] The invention relates to the technical field of deodorants, and in particular to a production process of a microbial deodorant. Background Art

[0002] In the field of chemistry and environmental engineering, microbial deodorants, as an emerging environmental protection technology, are gradually receiving widespread attention for their research and application background. With the rapid development of industrialization and urbanization, odor pollution has become one of the important challenges for improving environmental quality. Although traditional physical and chemical deodorization methods, such as adsorption, absorption, oxidation and masking, can alleviate the odor problem to a certain extent, they often have disadvantages such as low treatment efficiency, high operating costs, and easy secondary pollution. Therefore, it is urgent to develop an efficient, environmentally friendly and economical deodorization technology.

[0003] Microbial deodorants utilize the biodegradation of microorganisms to convert malodorous substances into harmless or low-harmful substances, such as carbon dioxide and water. This method has the advantages of good treatment effect, low operating cost, and no secondary pollution. It is particularly suitable for treating gases containing complex organic malodorous compounds. The core technology of microbial deodorants lies in the screening and cultivation of microbial strains with the ability to efficiently degrade malodorous substances, as well as the development of a suitable microbial growth and metabolic environment.

[0004] In recent years, the research on microbial deodorants has mainly focused on the following aspects: first, in-depth research on the microbial degradation mechanism of malodorous substances to guide the screening and cultivation of strains; second, optimization of the formulation and preparation process of microbial agents to improve the activity and stability of the agents; third, design and optimization of microbial deodorization systems to improve the treatment efficiency and adaptability of the system; fourth, exploration of the combined application of microbial deodorization technology with other technologies, such as combined use with biofiltration, biotrickling filtration and other technologies to improve the overall treatment effect.

[0005] Although microbial deodorant technology has made significant progress, it still faces some challenges. For example, there are many types of malodorous substances, and a single strain is difficult to cope with all types of malodorous substances; microorganisms are sensitive to environmental conditions, such as temperature, humidity, pH value, etc., which can affect the activity and degradation efficiency of microorganisms; in addition, the long-term operating stability and cost-benefit analysis of microbial deodorants are also issues that need to be considered in practical applications.

[0006] In summary, as an environmentally friendly technology with broad application prospects, the research and development of microbial deodorants is of great significance for improving environmental quality and achieving sustainable development. Future research needs to further explore the mechanism of microbial deodorants, optimize the bacterial agent and system design, and evaluate its performance and cost-effectiveness in practical applications to promote the commercialization and large-scale application of this technology. Summary of the invention

[0007] In view of the above-mentioned deficiencies in the prior art, the present invention provides a production process of a microbial deodorant.

[0008] In order to solve the above technical problems, the technical solution adopted by the present invention is: A production process of a microbial deodorant, characterized in that it comprises the following steps: (1) placing the composite fermentation liquid at room temperature and stirring at 120-200 rpm for 10-30 min to obtain a composite bacterial liquid; (2) Mix the composite bacterial liquid, microbial auxiliary agent and carrier according to the dosage ratio of 1 mL: (0.05-0.2) g: (0.8-1.2) g, stir at 120-200 rpm for 20-50 min, and dry at 35-42° C. to obtain a microbial deodorant; The microbial auxiliary agent is any one of yeast cell wall extract, plant fiber powder and protein powder; The carrier is modified porous medical stone.

[0009] Preferably, the composite fermentation broth is composed of Lactobacillus harbini fermentation broth, Sphingomonas zeae fermentation broth, Candida plicata fermentation broth, and Akkermansia muciniphila fermentation broth.

[0010] Furthermore, the mass ratio of the Harbin Lactobacillus fermentation broth, the Zea mays fermentation broth, the Candida plicata fermentation broth and the Akkermansia muciniphila fermentation broth is 1:(0.8-1.5):(1.2-2):(1.5-2.4).

[0011] The solid culture medium formula of Lactobacillus harbinensis, Sphingomonas zeae, Candida plicata and Akkermansia muciniphila is 1-4g of peptone, 0.5-2g of yeast extract powder, 0.1-0.5g of magnesium sulfate, 0.1-0.3g of manganese sulfate, 0.5-1.5g of dipotassium hydrogen phosphate, 8-15g of glucose, 8-15g of agar powder and 300-700ml of distilled water, which are mixed evenly and sterilized.

[0012] Harbin Lactobacillus is a lactic acid bacterium. It not only has a strong acid-producing ability, but can also produce lactic acid and other organic acids during metabolism, which can lower the pH value of the environment, thereby inhibiting the growth of odor-producing microorganisms. It can also metabolize certain malodorous substances, such as ammonia, hydrogen sulfide, etc., and convert them into less toxic or odorless substances.

[0013] Sphingomonas cornii has the ability to degrade a variety of organic pollutants, including some malodorous substances such as phenols and benzene; it can also secrete enzymes such as lactate dehydrogenase and alcohol dehydrogenase, which can catalyze the redox reactions of malodorous substances and promote their decomposition; it can also produce antioxidants to help neutralize the oxidative stress of malodorous substances.

[0014] Candida plicata is a yeast that can secrete lipase, which can break down malodorous lipid-containing substances such as volatile fatty acids. It has a high tolerance to environmental changes and can survive and function in harsh environments, making it suitable for use in deodorants. It has diverse metabolic pathways and can utilize a variety of carbon and nitrogen sources, which helps to decompose malodorous substances under different conditions.

[0015] The metabolites of Akkermansia muciniphila include short-chain fatty acids, such as acetic acid and propionic acid, which can inhibit the growth of malodorous microorganisms by improving the structure of intestinal microbial communities and promoting the growth of beneficial bacteria. They are able to degrade intestinal mucus, an ability that also enables them to decompose polysaccharides and proteins in malodorous substances.

[0016] In general, the reason why these microorganisms are used as deodorants is that they can directly decompose malodorous substances through metabolic activities, effectively decomposing major odorous gases such as ammonia, hydrogen sulfide, mercaptans and methyl mercaptan. At the same time, organic acids quickly reduce the pH value of the environment, thereby effectively inhibiting the proliferation of odor-producing microorganisms, fundamentally eliminating the production of malodorous gas substances, achieving in-situ source control of malodors, and achieving lasting elimination of odor sources. These microorganisms have a synergistic effect when used together: they have different metabolic pathways and can decompose various types of malodorous substances; when they work together, they can inhibit the growth of malodor-producing microorganisms through competitive exclusion mechanisms; they can secrete different enzymes, such as lactate dehydrogenase, lipase, etc., which can work together on malodorous substances to accelerate their decomposition; avoiding the long-term use of a single microorganism that may cause malodor-producing microorganisms to develop resistance to it.

[0017] The preparation method of the Harbin lactobacillus fermentation liquid is as follows: streaking the Harbin lactobacillus on a solid culture medium, culturing at 37-42° C. for 8-16 hours to obtain activated Harbin lactobacillus; inoculating the activated Harbin lactobacillus into a liquid culture medium A with an inoculation amount of 2-6wt%, culturing at 37-42° C. and 120-200rpm for 24-48 hours to obtain Harbin lactobacillus fermentation liquid; the preparation method of the liquid culture medium A is as follows: 8-14wt% skim milk powder, 1-4wt% glucose, 0.5-2wt% potassium dihydrogen phosphate, 0.5-2wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 6.3-7, and sterilizing.

[0018] The preparation method of the corn sphingomonas fermentation liquid is as follows: streaking the corn sphingomonas on a solid culture medium, culturing at 35-40° C. for 12-24 hours to obtain activated corn sphingomonas; inoculating the activated corn sphingomonas into a liquid culture medium B with an inoculation amount of 2-6wt%, culturing at 35-40° C. and 100-300rpm for 15-36 hours to obtain corn sphingomonas fermentation liquid; the preparation method of the liquid culture medium B is as follows: 3-8wt% of peptone, 10-14wt% of glucose, 0.5-2wt% of potassium dihydrogen phosphate, 0.5-2wt% of magnesium sulfate, and the remainder of distilled water, mixing them evenly, adjusting the pH of the solution to 7.0-7.5, and sterilizing.

[0019] The preparation method of the Candida plicata fermentation liquid is as follows: streaking the Candida plicata on a solid culture medium, culturing at 35-40° C. for 36-72 hours to obtain activated Candida plicata; inoculating the activated Candida plicata into a liquid culture medium C with an inoculation amount of 2-6wt%, culturing at 35-40° C. and 120-260rpm for 15-36 hours to obtain a Candida plicata fermentation liquid; the preparation method of the liquid culture medium C is as follows: 3-8wt% peptone, 10-14wt% glucose, 0.5-2wt% potassium dihydrogen phosphate, 0.5-2wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 7.0-7.5, and sterilizing.

[0020] The preparation method of the Akkermansia muciniphila fermentation broth is as follows: streaking Akkermansia muciniphila on a solid culture medium, culturing at 35-40° C. for 18-36 hours to obtain activated Akkermansia muciniphila; inoculating the activated Akkermansia muciniphila into a liquid culture medium D with an inoculation amount of 2-6wt%, culturing in a shaker at 35-40° C. for 18-28 hours to obtain Akkermansia muciniphila fermentation broth; the preparation method of the liquid culture medium D is as follows: 10-20wt% of beef extract, 2-5wt% of casein peptone, 0.5-2wt% of glucose, 0.5-2wt% of fucose, 0.1-0.5wt% of threonine, 0.1-0.3wt% of magnesium sulfate, and the remainder of distilled water are mixed evenly, the pH is adjusted to 6.0-7.0, and sterilized.

[0021] Modified porous medical stone is a material with high specific surface area and porous structure, which is usually modified by physical or chemical methods to enhance its adsorption and catalytic properties. Adding modified porous medical stone to microbial deodorants can further enhance the deodorization performance. The reasons and mechanisms are as follows: (1) The high specific surface area and porous structure of modified porous medical stone provide a large number of adsorption sites, which can adsorb pollutant molecules in malodorous gases, such as hydrogen sulfide, ammonia, volatile organic compounds, etc. This physical adsorption can quickly reduce the concentration of malodorous gases; (2) The porous structure provides additional attachment and growth space for microorganisms, increases the formation of biofilms, and may contain some catalytic active centers that can catalyze the redox reaction of malodorous substances and accelerate their decomposition, thereby increasing the biomass and metabolic activity of microorganisms, which helps to enhance the biodegradation ability of microorganisms for malodorous substances; (3) The stability and durability of modified porous medical stone can prolong the effective action time of microbial deodorants. Since porous medical stone is not easily degraded, it can continue to provide microbial attachment surfaces and adsorption sites for a long time, thereby improving the durability of the deodorant.

[0022] Preferably, the preparation method of the modified porous medical stone is as follows: The medical stone is soaked in 4-8wt% sodium hydroxide water for 15-30min at room temperature, washed, dried, crushed, and passed through a 60-100 mesh sieve to obtain porous medical stone; Add 100-400 parts by weight of water and 1-4 parts by weight of sodium lignin sulfonate to 15-30 parts by weight of the porous medical stone, and stir at 300-600 rpm for 30-60 min; then add 0.3-1 parts by weight of hexadecyltrimethylammonium bromide and 5-10 parts by weight of citric acid, continue stirring for 20-50 min to obtain a mixture, centrifuge, filter, and spray dry to obtain the modified porous medical stone.

[0023] The effective live bacteria count of Lactobacillus harbinensis in the microbial deodorant is ≥ 1×10 9 CFU / ml, effective viable count of Sphingomonas cornense ≥5×10 8 CFU / ml, effective viable count of Candida plicata ≥7×10 8 CFU / ml, effective viable count of Akkermansia muciniphila ≥4×10 8 CFU / ml.

[0024] A microbial deodorant is obtained by adopting any one of the above production processes.

[0025] Beneficial effects of the present invention: 1. The present invention provides a production process of a microbial deodorant. These microorganisms can directly decompose malodorous substances through metabolic activities, and effectively decompose major odorous gases such as ammonia, hydrogen sulfide, mercaptans and methyl mercaptan. At the same time, organic acids quickly reduce the pH value of the environment, thereby effectively inhibiting the proliferation of microorganisms that produce odor source gases, fundamentally eliminating the generation of malodorous gas substances, realizing in-situ source control of odor, and achieving long-term elimination of odor sources.

[0026] 2. Adding modified porous medical stone during the preparation process can not only adsorb pollutant molecules in malodorous gases, such as hydrogen sulfide, ammonia, volatile organic compounds, etc., but this physical adsorption can quickly reduce the concentration of malodorous gases; it also provides additional attachment and growth space for microorganisms, increases the formation of biofilms, and may contain some catalytic active centers that can catalyze the redox reactions of malodorous substances and accelerate their decomposition, thereby increasing the biomass and metabolic activity of microorganisms, which helps to enhance the biodegradation ability of microorganisms for malodorous substances.

[0027] 3. The microbial deodorant prepared by the present invention has good deodorizing effect and the deodorizing effect is durable. The preparation method is simple and suitable for large-scale promotion. DETAILED DESCRIPTION

[0028] The above content of the present invention is further described in detail below in conjunction with specific implementation modes, but this should not be understood as the scope of the above subject matter of the present invention being limited to the following embodiments.

[0029] Introduction of some raw materials in this application: Harbin Lactobacillus was provided by the General Microbiology Center of China Culture Collection Administration, with the strain number: CGMCC No.20473.

[0030] Sphingomonas maydis was provided by the General Microbiology Center of China Culture Collection Administration, with the strain number: CCTCC NO: M 2020193.

[0031] Candida plicata was provided by the General Microbiology Center of China Culture Collection Administration, with the strain number: CCTCC NO: M20231018.

[0032] Akkermansia muciniphila was provided by China Industrial Microbiological Culture Collection Center, strain number: CICC24917.

[0033] Example 1 A production process of a microbial deodorant comprises the following steps: (1) Lactobacillus harbinensis fermentation broth, Sphingomonas zeae fermentation broth, Candida plicatilis fermentation broth and Akkermansia muciniphila fermentation broth were mixed in a mass ratio of 1:1.2:1.5:2, and stirred at room temperature and 150 rpm for 15 minutes to obtain a composite bacterial solution; (2) The composite bacterial liquid, microbial auxiliary agent and carrier were mixed in a dosage ratio of 1 mL: 0.1 g: 1 g, stirred at 150 rpm for 30 min, and dried at 40° C. to obtain a microbial deodorant.

[0034] The effective viable bacteria count of Lactobacillus harbinensis in the microbial deodorant is 1.3×10 9 CFU / ml, effective viable count of Sphingomonas zeae was 5.9×10 8 CFU / ml, the effective viable count of Candida plicata was 7.5×10 8 CFU / ml, the effective viable count of Akkermansia muciniphila was 4.8×10 8 CFU / ml.

[0035] The solid culture medium formula of Lactobacillus harbinensis, Sphingomonas zeae, Candida plicata and Akkermansia muciniphila is 2g of peptone, 1g of yeast extract powder, 0.3g of magnesium sulfate, 0.2g of manganese sulfate, 0.8g of dipotassium hydrogen phosphate, 10g of glucose, 12g of agar powder and 500ml of distilled water, which are mixed evenly and sterilized.

[0036] The preparation method of the Harbin lactobacillus fermentation liquid is as follows: streaking Harbin lactobacillus on a solid culture medium, culturing at 40° C. for 12 hours to obtain activated Harbin lactobacillus; inoculating the activated Harbin lactobacillus into a liquid culture medium A with an inoculation amount of 4wt%, culturing at 40° C. and 150rpm for 36 hours to obtain Harbin lactobacillus fermentation liquid; the preparation method of the liquid culture medium A is as follows: 12wt% skim milk powder, 2wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 6.5, and sterilizing.

[0037] The preparation method of the corn sphingomonas fermentation liquid is as follows: streaking the corn sphingomonas on a solid culture medium, culturing at 37° C. for 16 hours to obtain activated corn sphingomonas; inoculating the activated corn sphingomonas into a liquid culture medium B with an inoculation amount of 4wt%, culturing at 37° C. and 200rpm for 20 hours to obtain corn sphingomonas fermentation liquid; the preparation method of the liquid culture medium B is as follows: 5wt% peptone, 12wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH of the solution is adjusted to 7.2, and sterilization is performed.

[0038] The preparation method of the Candida plicata fermentation liquid is as follows: streaking the Candida plicata on a solid culture medium, culturing at 37° C. for 48 hours to obtain activated Candida plicata; inoculating the activated Candida plicata into a liquid culture medium C with an inoculation amount of 4wt%, culturing at 37° C. and 200rpm for 48 hours to obtain a Candida plicata fermentation liquid; the preparation method of the liquid culture medium C is as follows: 5wt% peptone, 12wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH of the solution is adjusted to 7.2, and sterilization is performed.

[0039] The preparation method of the Akkermansia muciniphila fermentation broth is as follows: streaking Akkermansia muciniphila on a solid culture medium, culturing at 37°C for 24 hours to obtain activated Akkermansia muciniphila; inoculating the activated Akkermansia muciniphila into a liquid culture medium D with an inoculation amount of 4wt%, culturing in a shaker at 37°C for 24 hours to obtain Akkermansia muciniphila fermentation broth; the preparation method of the liquid culture medium D is as follows: 15wt% beef extract, 3wt% casein peptone, 1wt% glucose, 1wt% fucose, 0.2wt% threonine, 0.2wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH is adjusted to 6.5, and sterilized.

[0040] The microbial auxiliary agent is a yeast cell wall extract.

[0041] The carrier is modified porous medical stone, and the preparation method of the modified porous medical stone is as follows: The medical stone was soaked in 5wt% sodium hydroxide water at room temperature for 20min, washed, dried, crushed, and passed through an 80-mesh sieve to obtain porous medical stone; To 20 parts by weight of the porous medical stone, add 200 parts by weight of water and 2 parts by weight of sodium lignin sulfonate, and stir at 400 rpm for 40 minutes; then add 0.5 parts by weight of hexadecyltrimethylammonium bromide and 8 parts by weight of citric acid, and continue stirring for 30 minutes to obtain a mixture, which is centrifuged, filtered, and spray-dried to obtain the modified porous medical stone.

[0042] Example 2 A production process of a microbial deodorant comprises the following steps: (1) Lactobacillus harbinensis fermentation broth, Sphingomonas zeae fermentation broth, Candida plicatilis fermentation broth and Akkermansia muciniphila fermentation broth were mixed in a mass ratio of 1:0.8:1.2:1.5, and stirred at room temperature and 120 rpm for 10 minutes to obtain a composite bacterial solution; (2) The above-mentioned composite bacterial liquid, microbial auxiliary agent and carrier were mixed in a dosage ratio of 1 mL: 0.05 g: 0.8 g, stirred at 120 rpm for 20 min, and dried at 35° C. to obtain a microbial deodorant.

[0043] The effective viable bacteria count of Lactobacillus harbinensis in the microbial deodorant is 1.24×10 9 CFU / ml, the effective viable count of Sphingomonas zeae was 5.71×10 8 CFU / ml, the effective viable count of Candida plicatilis was 7.38×10 8 CFU / ml, the effective viable count of Akkermansia muciniphila was 4.61×10 8 CFU / ml.

[0044] The solid culture medium formula of Lactobacillus harbinensis, Sphingomonas zeae, Candida plicata and Akkermansia muciniphila is 1g of peptone, 0.5g of yeast extract powder, 0.1g of magnesium sulfate, 0.1g of manganese sulfate, 0.5g of dipotassium hydrogen phosphate, 8g of glucose, 8g of agar powder and 300ml of distilled water, which are mixed evenly and sterilized.

[0045] The preparation method of the Harbin lactobacillus fermentation liquid is as follows: streaking the Harbin lactobacillus on a solid culture medium, culturing at 37° C. for 8 hours to obtain activated Harbin lactobacillus; inoculating the activated Harbin lactobacillus into a liquid culture medium A with an inoculation amount of 2wt%, culturing at 37° C. and 120rpm for 24 hours to obtain Harbin lactobacillus fermentation liquid; the preparation method of the liquid culture medium A is as follows: 8wt% skim milk powder, 1wt% glucose, 0.5wt% potassium dihydrogen phosphate, 0.5wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 6.3, and sterilizing.

[0046] The preparation method of the corn sphingomonas fermentation liquid is as follows: streaking the corn sphingomonas on a solid culture medium, culturing at 35° C. for 12 hours to obtain activated corn sphingomonas; inoculating the activated corn sphingomonas into a liquid culture medium B with an inoculation amount of 2wt%, culturing at 35° C. and 100rpm for 15 hours to obtain the corn sphingomonas fermentation liquid; the preparation method of the liquid culture medium B is as follows: 3wt% of peptone, 10wt% of glucose, 0.5wt% of potassium dihydrogen phosphate, 0.5wt% of magnesium sulfate, and the remainder of distilled water are mixed evenly, the pH of the solution is adjusted to 7.0, and sterilization is performed.

[0047] The preparation method of the Candida plicata fermentation liquid is as follows: streaking the Candida plicata on a solid culture medium, culturing at 35° C. for 36 hours to obtain activated Candida plicata; inoculating the activated Candida plicata into a liquid culture medium C with an inoculation amount of 2wt%, culturing at 35° C. and 120rpm for 36 hours to obtain a Candida plicata fermentation liquid; the preparation method of the liquid culture medium C is as follows: 3wt% peptone, 10wt% glucose, 0.5wt% potassium dihydrogen phosphate, 0.5wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 7.0, and sterilizing.

[0048] The preparation method of the Akkermansia muciniphila fermentation broth is as follows: streaking Akkermansia muciniphila on a solid culture medium, culturing at 35°C for 18 hours to obtain activated Akkermansia muciniphila; inoculating the activated Akkermansia muciniphila into a liquid culture medium D with an inoculation amount of 2wt%, culturing in a shaker at 35°C for 18 hours to obtain Akkermansia muciniphila fermentation broth; the preparation method of the liquid culture medium D is as follows: 10wt% beef extract, 2wt% casein peptone, 0.5wt% glucose, 0.5wt% fucose, 0.1wt% threonine, 0.1wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH is adjusted to 6.0, and sterilized.

[0049] The microbial auxiliary agent is a yeast cell wall extract.

[0050] The carrier is modified porous medical stone, and the preparation method of the modified porous medical stone is as follows: The medical stone was soaked in 4wt% sodium hydroxide water at room temperature for 15min, washed, dried, crushed, and passed through a 60-mesh sieve to obtain porous medical stone; To 15 parts by weight of the porous medical stone, 100 parts by weight of water and 1 part by weight of sodium lignin sulfonate were added, and the mixture was stirred at 300 rpm for 30 min; then 0.3 parts by weight of hexadecyltrimethylammonium bromide and 5 parts by weight of citric acid were added, and the mixture was stirred for 20 min. The mixture was centrifuged, filtered, and spray-dried to obtain the modified porous medical stone.

[0051] Example 3 A production process of a microbial deodorant comprises the following steps: (1) Lactobacillus harbinensis fermentation broth, Sphingomonas zeae fermentation broth, Candida plicatilis fermentation broth and Akkermansia muciniphila fermentation broth were mixed in a mass ratio of 1:1.5:2:2.4, and stirred at room temperature and 200 rpm for 30 minutes to obtain a composite bacterial solution; (2) The above-mentioned composite bacterial liquid, microbial auxiliary agent and carrier were mixed in a dosage ratio of 1 mL: 0.2 g: 1.2 g, stirred at 200 rpm for 50 min, and dried at 42° C. to obtain a microbial deodorant.

[0052] The effective viable bacteria count of Lactobacillus harbinensis in the microbial deodorant is 1.27×10 9 CFU / ml, the effective viable count of Sphingomonas zeae was 5.74×10 8 CFU / ml, the effective viable count of Candida plicatilis was 7.39×10 8 CFU / ml, the effective viable count of Akkermansia muciniphila was 4.68×10 8 CFU / ml.

[0053] The solid culture medium formula of Lactobacillus harbinensis, Sphingomonas zeae, Candida plicata and Akkermansia muciniphila is 4g of peptone, 2g of yeast extract powder, 0.5g of magnesium sulfate, 0.3g of manganese sulfate, 1.5g of dipotassium hydrogen phosphate, 15g of glucose, 15g of agar powder and 700ml of distilled water, which are mixed evenly and sterilized.

[0054] The preparation method of the Harbin lactobacillus fermentation liquid is as follows: streaking Harbin lactobacillus on a solid culture medium, culturing at 42° C. for 16 hours to obtain activated Harbin lactobacillus; inoculating the activated Harbin lactobacillus into a liquid culture medium A with an inoculation amount of 6wt%, culturing at 42° C. and 200rpm for 48 hours to obtain Harbin lactobacillus fermentation liquid; the preparation method of the liquid culture medium A is as follows: 14wt% skim milk powder, 4wt% glucose, 2wt% potassium dihydrogen phosphate, 2wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 7.0, and sterilizing.

[0055] The preparation method of the corn sphingomonas fermentation liquid is as follows: streaking the corn sphingomonas on a solid culture medium, culturing at 40° C. for 24 hours to obtain activated corn sphingomonas; inoculating the activated corn sphingomonas into a liquid culture medium B with an inoculation amount of 6wt%, culturing at 40° C. and 300rpm for 36 hours to obtain the corn sphingomonas fermentation liquid; the preparation method of the liquid culture medium B is as follows: 8wt% of peptone, 14wt% of glucose, 2wt% of potassium dihydrogen phosphate, 2wt% of magnesium sulfate, and the remainder of distilled water are mixed evenly, the pH of the solution is adjusted to 7.5, and sterilization is performed.

[0056] The preparation method of the Candida plicata fermentation liquid is as follows: streaking the Candida plicata on a solid culture medium, culturing at 40° C. for 72 hours to obtain activated Candida plicata; inoculating the activated Candida plicata into a liquid culture medium C with an inoculation amount of 6wt%, culturing at 40° C. and 260rpm for 72 hours to obtain a Candida plicata fermentation liquid; the preparation method of the liquid culture medium C is as follows: 8wt% peptone, 14wt% glucose, 2wt% potassium dihydrogen phosphate, 2wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH of the solution is adjusted to 7.5, and sterilization is performed.

[0057] The preparation method of the Akkermansia muciniphila fermentation broth is as follows: streaking Akkermansia muciniphila on a solid culture medium, culturing at 40°C for 36 hours to obtain activated Akkermansia muciniphila; inoculating the activated Akkermansia muciniphila into a liquid culture medium D with an inoculation amount of 6wt%, culturing in a shaker at 40°C for 28 hours to obtain Akkermansia muciniphila fermentation broth; the preparation method of the liquid culture medium D is as follows: 20wt% of beef extract, 5wt% of casein peptone, 2wt% of glucose, 2wt% of fucose, 0.5wt% of threonine, 0.3wt% of magnesium sulfate, and the remainder of distilled water are mixed evenly, the pH is adjusted to 7.0, and sterilized.

[0058] The microbial auxiliary agent is a yeast cell wall extract.

[0059] The carrier is modified porous medical stone, and the preparation method of the modified porous medical stone is as follows: The medical stone was soaked in 8wt% sodium hydroxide water at room temperature for 30min, washed, dried, crushed, and passed through a 100-mesh sieve to obtain porous medical stone; To 30 parts by weight of the porous medical stone, 400 parts by weight of water and 4 parts by weight of sodium lignin sulfonate were added, and the mixture was stirred at 600 rpm for 60 min; then 1 part by weight of hexadecyltrimethylammonium bromide and 10 parts by weight of citric acid were added, and the mixture was stirred for 50 min. The mixture was centrifuged, filtered, and spray-dried to obtain the modified porous medical stone.

[0060] Comparative Example 1 A production process of a microbial deodorant comprises the following steps: (1) Lactobacillus harbinensis fermentation broth, Sphingomonas zeae fermentation broth, Candida plicatilis fermentation broth and Akkermansia muciniphila fermentation broth were mixed in a mass ratio of 1:1.2:1.5:2, and stirred at room temperature and 150 rpm for 15 minutes to obtain a composite bacterial solution; (2) The composite bacterial solution and the carrier were mixed in a ratio of 1 mL: 0.1 g: 1 g, stirred at 150 rpm for 30 min, and dried at 40° C. to obtain a microbial deodorant.

[0061] The effective viable bacteria count of Lactobacillus harbinensis in the microbial deodorant is 0.81×10 9 CFU / ml, the effective viable count of Sphingomonas zeae was 4.18×10 8 CFU / ml, the effective viable count of Candida plicatilis was 5.82×10 8 CFU / ml, the effective viable count of Akkermansia muciniphila was 3.27×10 8 CFU / ml.

[0062] The solid culture medium formula of Lactobacillus harbinensis, Sphingomonas zeae, Candida plicata and Akkermansia muciniphila is 2g of peptone, 1g of yeast extract powder, 0.3g of magnesium sulfate, 0.2g of manganese sulfate, 0.8g of dipotassium hydrogen phosphate, 10g of glucose, 12g of agar powder and 500ml of distilled water, which are mixed evenly and sterilized.

[0063] The preparation method of the Harbin lactobacillus fermentation liquid is as follows: streaking Harbin lactobacillus on a solid culture medium, culturing at 40° C. for 12 hours to obtain activated Harbin lactobacillus; inoculating the activated Harbin lactobacillus into a liquid culture medium A with an inoculation amount of 4wt%, culturing at 40° C. and 150rpm for 36 hours to obtain Harbin lactobacillus fermentation liquid; the preparation method of the liquid culture medium A is as follows: 12wt% skim milk powder, 2wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 6.5, and sterilizing.

[0064] The preparation method of the corn sphingomonas fermentation liquid is as follows: streaking the corn sphingomonas on a solid culture medium, culturing at 37° C. for 16 hours to obtain activated corn sphingomonas; inoculating the activated corn sphingomonas into a liquid culture medium B with an inoculation amount of 4wt%, culturing at 37° C. and 200rpm for 20 hours to obtain corn sphingomonas fermentation liquid; the preparation method of the liquid culture medium B is as follows: 5wt% peptone, 12wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH of the solution is adjusted to 7.2, and sterilization is performed.

[0065] The preparation method of the Candida plicata fermentation liquid is as follows: streaking the Candida plicata on a solid culture medium, culturing at 37° C. for 48 hours to obtain activated Candida plicata; inoculating the activated Candida plicata into a liquid culture medium C with an inoculation amount of 4wt%, culturing at 37° C. and 200rpm for 48 hours to obtain a Candida plicata fermentation liquid; the preparation method of the liquid culture medium C is as follows: 5wt% peptone, 12wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH of the solution is adjusted to 7.2, and sterilization is performed.

[0066] The preparation method of the Akkermansia muciniphila fermentation broth is as follows: streaking Akkermansia muciniphila on a solid culture medium, culturing at 37°C for 24 hours to obtain activated Akkermansia muciniphila; inoculating the activated Akkermansia muciniphila into a liquid culture medium D with an inoculation amount of 4wt%, culturing in a shaker at 37°C for 24 hours to obtain Akkermansia muciniphila fermentation broth; the preparation method of the liquid culture medium D is as follows: 15wt% beef extract, 3wt% casein peptone, 1wt% glucose, 1wt% fucose, 0.2wt% threonine, 0.2wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH is adjusted to 6.5, and sterilized.

[0067] The carrier is medical stone.

[0068] Comparative Example 2 A production process of a microbial deodorant comprises the following steps: (1) Lactobacillus harbinensis fermentation broth, Sphingomonas zeae fermentation broth, Candida plicatilis fermentation broth and Akkermansia muciniphila fermentation broth were mixed in a mass ratio of 1:1.2:1.5:2, and stirred at room temperature and 150 rpm for 15 minutes to obtain a composite bacterial solution; (2) The composite bacterial liquid, microbial auxiliary agent and carrier were mixed in a dosage ratio of 1 mL: 0.1 g: 1 g, stirred at 150 rpm for 30 min, and dried at 40° C. to obtain a microbial deodorant.

[0069] The effective viable bacteria count of Lactobacillus harbinensis in the microbial deodorant is 0.84×10 9 CFU / ml, the effective viable count of Sphingomonas zeae was 4.21×10 8 CFU / ml, the effective viable count of Candida plicatilis was 5.87×10 8 CFU / ml, the effective viable count of Akkermansia muciniphila was 3.29×10 8 CFU / ml.

[0070] The solid culture medium formula of Lactobacillus harbinensis, Sphingomonas zeae, Candida plicata and Akkermansia muciniphila is 2g of peptone, 1g of yeast extract powder, 0.3g of magnesium sulfate, 0.2g of manganese sulfate, 0.8g of dipotassium hydrogen phosphate, 10g of glucose, 12g of agar powder and 500ml of distilled water, which are mixed evenly and sterilized.

[0071] The preparation method of the Harbin lactobacillus fermentation liquid is as follows: streaking Harbin lactobacillus on a solid culture medium, culturing at 40° C. for 12 hours to obtain activated Harbin lactobacillus; inoculating the activated Harbin lactobacillus into a liquid culture medium A with an inoculation amount of 4wt%, culturing at 40° C. and 150rpm for 36 hours to obtain Harbin lactobacillus fermentation liquid; the preparation method of the liquid culture medium A is as follows: 12wt% skim milk powder, 2wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 6.5, and sterilizing.

[0072] The preparation method of the corn sphingomonas fermentation liquid is as follows: streaking the corn sphingomonas on a solid culture medium, culturing at 37° C. for 16 hours to obtain activated corn sphingomonas; inoculating the activated corn sphingomonas into a liquid culture medium B with an inoculation amount of 4wt%, culturing at 37° C. and 200rpm for 20 hours to obtain corn sphingomonas fermentation liquid; the preparation method of the liquid culture medium B is as follows: 5wt% peptone, 12wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH of the solution is adjusted to 7.2, and sterilization is performed.

[0073] The preparation method of the Candida plicata fermentation liquid is as follows: streaking the Candida plicata on a solid culture medium, culturing at 37° C. for 48 hours to obtain activated Candida plicata; inoculating the activated Candida plicata into a liquid culture medium C with an inoculation amount of 4wt%, culturing at 37° C. and 200rpm for 48 hours to obtain a Candida plicata fermentation liquid; the preparation method of the liquid culture medium C is as follows: 5wt% peptone, 12wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH of the solution is adjusted to 7.2, and sterilization is performed.

[0074] The preparation method of the Akkermansia muciniphila fermentation broth is as follows: streaking Akkermansia muciniphila on a solid culture medium, culturing at 37°C for 24 hours to obtain activated Akkermansia muciniphila; inoculating the activated Akkermansia muciniphila into a liquid culture medium D with an inoculation amount of 4wt%, culturing in a shaker at 37°C for 24 hours to obtain Akkermansia muciniphila fermentation broth; the preparation method of the liquid culture medium D is as follows: 15wt% beef extract, 3wt% casein peptone, 1wt% glucose, 1wt% fucose, 0.2wt% threonine, 0.2wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH is adjusted to 6.5, and sterilized.

[0075] The microbial auxiliary agent is a yeast cell wall extract.

[0076] The carrier is medical stone.

[0077] Comparative Example 3 A production process of a microbial deodorant comprises the following steps: (1) Lactobacillus harbinensis fermentation broth, Sphingomonas zeae fermentation broth, Candida plicatilis fermentation broth and Akkermansia muciniphila fermentation broth were mixed in a mass ratio of 1:1.2:1.5:2, and stirred at room temperature and 150 rpm for 15 minutes to obtain a composite bacterial solution; (2) The composite bacterial liquid, microbial auxiliary agent and carrier were mixed in a dosage ratio of 1 mL: 0.1 g: 1 g, stirred at 150 rpm for 30 min, and dried at 40° C. to obtain a microbial deodorant.

[0078] The effective viable bacteria count of Lactobacillus harbinensis in the microbial deodorant is 0.89×10 9 CFU / ml, the effective viable count of Sphingomonas zeae was 4.87×10 8 CFU / ml, the effective viable count of Candida plicata was 6.42×10 8 CFU / ml, the effective viable count of Akkermansia muciniphila was 3.93×10 8 CFU / ml.

[0079] The solid culture medium formula of Lactobacillus harbinensis, Sphingomonas zeae, Candida plicata and Akkermansia muciniphila is 2g of peptone, 1g of yeast extract powder, 0.3g of magnesium sulfate, 0.2g of manganese sulfate, 0.8g of dipotassium hydrogen phosphate, 10g of glucose, 12g of agar powder and 500ml of distilled water, which are mixed evenly and sterilized.

[0080] The preparation method of the Harbin lactobacillus fermentation liquid is as follows: streaking Harbin lactobacillus on a solid culture medium, culturing at 40° C. for 12 hours to obtain activated Harbin lactobacillus; inoculating the activated Harbin lactobacillus into a liquid culture medium A with an inoculation amount of 4wt%, culturing at 40° C. and 150rpm for 36 hours to obtain Harbin lactobacillus fermentation liquid; the preparation method of the liquid culture medium A is as follows: 12wt% skim milk powder, 2wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 6.5, and sterilizing.

[0081] The preparation method of the corn sphingomonas fermentation liquid is as follows: streaking the corn sphingomonas on a solid culture medium, culturing at 37° C. for 16 hours to obtain activated corn sphingomonas; inoculating the activated corn sphingomonas into a liquid culture medium B with an inoculation amount of 4wt%, culturing at 37° C. and 200rpm for 20 hours to obtain corn sphingomonas fermentation liquid; the preparation method of the liquid culture medium B is as follows: 5wt% peptone, 12wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH of the solution is adjusted to 7.2, and sterilization is performed.

[0082] The preparation method of the Candida plicata fermentation liquid is as follows: streaking the Candida plicata on a solid culture medium, culturing at 37° C. for 48 hours to obtain activated Candida plicata; inoculating the activated Candida plicata into a liquid culture medium C with an inoculation amount of 4wt%, culturing at 37° C. and 200rpm for 48 hours to obtain a Candida plicata fermentation liquid; the preparation method of the liquid culture medium C is as follows: 5wt% peptone, 12wt% glucose, 1wt% potassium dihydrogen phosphate, 1wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH of the solution is adjusted to 7.2, and sterilization is performed.

[0083] The preparation method of the Akkermansia muciniphila fermentation broth is as follows: streaking Akkermansia muciniphila on a solid culture medium, culturing at 37°C for 24 hours to obtain activated Akkermansia muciniphila; inoculating the activated Akkermansia muciniphila into a liquid culture medium D with an inoculation amount of 4wt%, culturing in a shaker at 37°C for 24 hours to obtain Akkermansia muciniphila fermentation broth; the preparation method of the liquid culture medium D is as follows: 15wt% beef extract, 3wt% casein peptone, 1wt% glucose, 1wt% fucose, 0.2wt% threonine, 0.2wt% magnesium sulfate, and the remainder distilled water are mixed evenly, the pH is adjusted to 6.5, and sterilized.

[0084] The microbial auxiliary agent is a yeast cell wall extract.

[0085] The carrier is porous medical stone, and the preparation method of the porous medical stone is as follows: The medical stone was soaked in 5wt% sodium hydroxide water at room temperature for 20 minutes, washed, dried, crushed, and passed through an 80-mesh sieve to obtain porous medical stone.

[0086] Test Example 1 Deodorization detection method: 1 kg of kitchen waste was used as the odor source, which was divided into 10L trash cans, and the microbial deodorants of Examples 1-3 and Comparative Examples 1-3 were added, and the amount added was 100 g / kg. An equal amount of pure water was added to the control group, and the bags were sealed with plastic bags and placed in an environment of 30°C. The contents of ammonia, hydrogen sulfide and volatile organic compounds (VOCs) were measured every 24 hours. The experimental period was 48 hours. The deodorization effect is shown in Table 1.

[0087] Table 1 Deodorization test results of microbial deodorants Ammonia removal rate / % Hydrogen sulfide removal rate / % Volatile organic matter removal rate / % Example 1 89.3 97.6 76.2 Example 2 86.2 94.1 70.6 Example 3 87.1 96.5 72.5 Comparative Example 1 52.9 63.5 41.3 Comparative Example 2 67.4 76.9 50.7 Comparative Example 3 73.2 83.7 62.3 Control group 20.6 18.3 8.4 Test Example 2 Deodorant persistence test: The microbial deodorants prepared by the methods of Example 1 and Comparative Examples 1-3 were filled into a 500 mL opaque plastic bottle, and placed at room temperature and 4°C for 1 month, respectively. Deodorization was then performed according to the method of the test example. The test results are shown in Table 2.

[0088] Table 2 Deodorization test results of microbial deodorants

[0089] From the above results, it can be seen that the microbial deodorant prepared by the present invention has a good deodorizing effect, and the deodorizing effect is durable. The reason is that Harbin Lactobacillus belongs to lactic acid bacteria, which not only has a strong acid-producing ability, but also can produce lactic acid and other organic acids during metabolism, which can reduce the pH value of the environment, thereby inhibiting the growth of odor-producing microorganisms; and can metabolize certain odorous substances, such as ammonia, hydrogen sulfide, etc., and convert them into less toxic or odorless substances. Zea mays Sphingomonas has the ability to degrade a variety of organic pollutants, including some odorous substances, such as phenols, benzene, etc.; it can also secrete enzymes such as lactate dehydrogenase and alcohol dehydrogenase, which can catalyze the redox reaction of odorous substances and promote their decomposition; it can also produce antioxidants, which help to neutralize the oxidative stress of odorous substances. Candida plicata is a yeast that can secrete lipases, which can break down lipid-containing malodorous substances, such as volatile fatty acids. It has a high tolerance to environmental changes and can survive and function in harsh environments, making it suitable for use in deodorants. It has diverse metabolic pathways and can utilize a variety of carbon and nitrogen sources, which helps to break down malodorous substances under different conditions. The metabolites of Akkermansia muciniphila include short-chain fatty acids, such as acetic acid and propionic acid, which can inhibit the growth of malodorous microorganisms by improving the structure of intestinal microbial communities and promoting the growth of beneficial bacteria. It can degrade intestinal mucus, and this ability also enables it to break down polysaccharides and proteins in malodorous substances.

[0090] In summary, the reason why these microorganisms are used as deodorants is that they can directly decompose malodorous substances through metabolic activities, effectively decomposing major odorous gases such as ammonia, hydrogen sulfide, mercaptans and methyl mercaptan. At the same time, organic acids quickly reduce the pH value of the environment, thereby effectively inhibiting the proliferation of odor-producing microorganisms, fundamentally eliminating the production of malodorous gas substances, achieving in-situ source control of malodors, and achieving lasting elimination of odor sources. These microorganisms have a synergistic effect when used together: they have different metabolic pathways and can decompose various types of malodorous substances; when they work together, they can inhibit the growth of malodor-producing microorganisms through competitive exclusion mechanisms; they can secrete different enzymes, such as lactate dehydrogenase, lipase, etc., which can work together on malodorous substances to accelerate their decomposition; avoiding the long-term use of a single microorganism that may cause malodor-producing microorganisms to develop resistance to it.

[0091] Adding modified porous medical stone to microbial deodorants can further enhance the deodorizing performance for the following reasons: (1) The high specific surface area and porous structure of modified porous medical stone provide a large number of adsorption sites, which can adsorb pollutant molecules in malodorous gases, such as hydrogen sulfide, ammonia, volatile organic compounds, etc. This physical adsorption can quickly reduce the concentration of malodorous gases; (2) The porous structure provides additional attachment and growth space for microorganisms, increases the formation of biofilms, and may contain some catalytic active centers that can catalyze the redox reactions of malodorous substances and accelerate their decomposition, thereby increasing the biomass and metabolic activity of microorganisms, which helps to enhance the biodegradation ability of microorganisms for malodorous substances; (3) The stability and durability of modified porous medical stone can prolong the effective action time of microbial deodorants. Since porous medical stone is not easily degraded, it can continue to provide microbial attachment surfaces and adsorption sites for a long time, thereby improving the durability of the deodorant.

Claims

1. A production process of a microbial deodorant, characterized in that: The steps include: (1) stirring the composite fermentation liquid at room temperature to obtain a composite bacterial liquid; (2) Mixing the composite bacterial liquid, microbial auxiliary agent and carrier in a ratio of 1 mL: (0.05-0.2) g: (0.8-1.2) g, stirring and drying to obtain a microbial deodorant; The microbial auxiliary agent is any one of yeast cell wall extract, plant fiber powder and protein powder; The carrier is modified porous medical stone.

2. The production process of the microbial deodorant according to claim 1, characterized in that: The mass ratio of the Harbin Lactobacillus fermentation broth, the Zea mays Sphingomonas fermentation broth, the Candida plicata fermentation broth and the Akkermansia muciniphila fermentation broth is 1:(0.8-1.5):(1.2-2):(1.5-2.4).

3. The production process of the microbial deodorant according to claim 2, characterized in that: The solid culture medium formula of Lactobacillus harbinensis, Sphingomonas zeae, Candida plicata and Akkermansia muciniphila is 1-4g of peptone, 0.5-2g of yeast extract powder, 0.1-0.5g of magnesium sulfate, 0.1-0.3g of manganese sulfate, 0.5-1.5g of dipotassium hydrogen phosphate, 8-15g of glucose, 8-15g of agar powder and 300-700ml of distilled water, which are mixed evenly and sterilized.

4. The production process of the microbial deodorant according to claim 2, characterized in that: The preparation method of the Harbin lactobacillus fermentation liquid is as follows: streaking the Harbin lactobacillus on a solid culture medium, culturing at 37-42° C. for 8-16 hours to obtain activated Harbin lactobacillus; inoculating the activated Harbin lactobacillus into a liquid culture medium A with an inoculation amount of 2-6wt%, culturing at 37-42° C. and 120-200rpm for 24-48 hours to obtain Harbin lactobacillus fermentation liquid; the preparation method of the liquid culture medium A is as follows: 8-14wt% skim milk powder, 1-4wt% glucose, 0.5-2wt% potassium dihydrogen phosphate, 0.5-2wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 6.3-7, and sterilizing.

5. The production process of the microbial deodorant according to claim 2, characterized in that: The preparation method of the corn sphingomonas fermentation liquid is as follows: streaking the corn sphingomonas on a solid culture medium, culturing at 35-40° C. for 12-24 hours to obtain activated corn sphingomonas; inoculating the activated corn sphingomonas into a liquid culture medium B with an inoculation amount of 2-6wt%, culturing at 35-40° C. and 100-300rpm for 15-36 hours to obtain corn sphingomonas fermentation liquid; the preparation method of the liquid culture medium B is as follows: 3-8wt% of peptone, 10-14wt% of glucose, 0.5-2wt% of potassium dihydrogen phosphate, 0.5-2wt% of magnesium sulfate, and the remainder of distilled water, mixing them evenly, adjusting the pH of the solution to 7.0-7.5, and sterilizing.

6. The production process of the microbial deodorant according to claim 2, characterized in that: The preparation method of the Candida plicata fermentation liquid is as follows: streaking the Candida plicata on a solid culture medium, culturing at 35-40° C. for 36-72 hours to obtain activated Candida plicata; inoculating the activated Candida plicata into a liquid culture medium C with an inoculation amount of 2-6wt%, culturing at 35-40° C. and 120-260rpm for 15-36 hours to obtain a Candida plicata fermentation liquid; the preparation method of the liquid culture medium C is as follows: 3-8wt% peptone, 10-14wt% glucose, 0.5-2wt% potassium dihydrogen phosphate, 0.5-2wt% magnesium sulfate, and the remainder distilled water, mixing evenly, adjusting the solution pH to 7.0-7.5, and sterilizing.

7. The production process of the microbial deodorant according to claim 2, characterized in that: The preparation method of the Akkermansia muciniphila fermentation broth is as follows: streaking Akkermansia muciniphila on a solid culture medium, culturing at 35-40° C. for 18-36 hours to obtain activated Akkermansia muciniphila; inoculating the activated Akkermansia muciniphila into a liquid culture medium D with an inoculation amount of 2-6wt%, culturing in a shaker at 35-40° C. for 18-28 hours to obtain Akkermansia muciniphila fermentation broth; the preparation method of the liquid culture medium D is as follows: 10-20wt% of beef extract, 2-5wt% of casein peptone, 0.5-2wt% of glucose, 0.5-2wt% of fucose, 0.1-0.5wt% of threonine, 0.1-0.3wt% of magnesium sulfate, and the remainder of distilled water are mixed evenly, the pH is adjusted to 6.0-7.0, and sterilized.

8. The production process of the microbial deodorant according to claim 2, characterized in that: The effective live bacteria count of Lactobacillus harbinensis in the microbial deodorant is ≥ 1×10 9 CFU / ml, effective viable count of Sphingomonas cornense ≥5×10 8 CFU / ml, effective viable count of Candida plicata ≥7×10 8 CFU / ml, effective viable count of Akkermansia muciniphila ≥4×10 8 CFU / ml.

9. The production process of the microbial deodorant according to claim 1, characterized in that: The preparation method of the modified porous medical stone is as follows: The medical stone is soaked in 4-8wt% sodium hydroxide water for 15-30min at room temperature, washed, dried, crushed, and passed through a 60-100 mesh sieve to obtain porous medical stone; Add 100-400 parts by weight of water and 1-4 parts by weight of sodium lignin sulfonate to 15-30 parts by weight of the porous medical stone and stir; then add 0.3-1 parts by weight of hexadecyltrimethylammonium bromide and 5-10 parts by weight of citric acid, continue stirring to obtain a mixture, centrifuge, filter, and spray-dry to obtain the modified porous medical stone.

10. A microbial deodorant obtained by the production process according to any one of claims 1 to 9.

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