Fermentation temperature control method and system
By integrating bionic sensors and microwave detection technology, combined with dynamic thermal production model and interlaced magnetic field adjustment, precise control of the fermentation temperature of tangerine peel is achieved, solving the problems of metabolic imbalance of bacterial flora and uneven product distribution, and improving the uniformity and quality stability of the product.
Patent Information
- Application Number
- CN202510354198.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-25
- Publication Date
- 2025-04-22
- Estimated Expiration
- 2045-03-25
AI Technical Summary
The traditional tangerine peel fermentation process is difficult to accurately control the fermentation temperature, resulting in imbalance in the metabolism of bacterial flora and uneven distribution of secondary metabolites, and it is impossible to adapt to the dynamic heat production rules of the growth stage of bacterial flora in real time.
The integrated bionic sensor array and microwave moisture content detection technology are used to obtain the thickening rate of the mucus layer in the fermentation reactor and the distribution of the material's self-weight pressure, and calculate the total amount of biological metabolic heat production based on the dynamic heat production model. Through the three-stage heating program and interleaved magnetic field adjustment, the temperature distribution of the fermentation reactor is dynamically adjusted, the bacterial distribution is guided and the temperature gradient is optimized.
Accurate control of fermentation temperature is achieved, the problems of imbalance in bacterial metabolism and uneven distribution of secondary metabolites are solved, and the uniformity and quality stability of the product are improved.
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Figure CN119861773A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of tangerine peel fermentation and relates to a fermentation temperature control method and system. Background Art
[0002] In the tangerine peel fermentation process, the dynamic temperature control of the material directly affects the bacterial metabolic pathway and the production efficiency of the target components. Traditional technologies generally rely on manual experience to set a fixed temperature curve, which is difficult to accurately respond to the temperature distribution imbalance caused by the fluctuation of bacterial activity, the sudden change of environmental temperature and humidity, and the heterogeneity of material stratification during the fermentation process. Especially in the deep fermentation pile, the mucus layer formed by the extrusion of the material's own weight hinders the diffusion of heat, resulting in local overheating and regional bacterial metabolic imbalance, which directly affects the uniformity of secondary metabolites.
[0003] Existing technologies build simple feedback systems by installing temperature control devices and conventional sensors. For example, thermocouple temperature control devices are placed in the fermentation box to trigger heating or ventilation based on thresholds. Some optimization methods introduce humidity sensors to adjust the damper opening, or use mechanical stirring to promote heat homogenization. Such solutions rely on linear control logic. Although they can alleviate local overheating, they lack the ability to model the dynamic calculation of heat production by bacterial metabolism, the change in thermal resistance caused by the thickening of the mucus layer, and the nonlinear correlation of multi-parameter coupling to the temperature field.
[0004] Based on the above problems, the disadvantage of the traditional method is that its underlying control logic does not integrate material characteristics and microbial metabolic kinetics models, resulting in data fragmentation and insufficient prediction accuracy; it is unable to adapt to the dynamic heat production laws of the bacterial growth stage in real time, causing excessive heating or ineffective heat dissipation. Summary of the invention
[0005] In order to solve the above problems, the present invention provides a fermentation temperature control method and system.
[0006] In a first aspect, the present invention provides a fermentation temperature control method, which adopts the following technical scheme: A fermentation temperature control method comprises the following steps: S1. Obtaining the anti-pollution treatment data of the material, including arranging an anti-pollution sensor with a bionic groove structure, detecting the moisture content of the mucus layer in the material, and calculating the total amount of heat generated by biological metabolism in combination with the deadweight pressure of the material; S2. Triggering the hierarchical temperature control program according to the total amount of heat produced by biological metabolism, adjusting the temperature distribution of the fermentation pile through the coupling control of the three-stage heating and dynamic maintenance stages; S3, generating a staggered magnetic field according to the temperature gradient distribution inside the material, guiding the surface bacteria to migrate to the middle and upper high magnetic field area, and inhibiting the aggregation concentration of the bottom heat-producing bacteria; S4, when it is detected that the moisture content of the middle layer exceeds the threshold, the directional water absorption program of the moisture-conducting membrane is activated and the aromatic substances in the overflowed liquid are separated and recovered; S5. Release the aroma capture particles according to the sudden change data of the ambient humidity, and simultaneously open the directional heat dissipation duct to balance the temperature difference of the material layer; S6. Analyze the actual yield of the target component in the finished product and optimize the initial control parameters for the next batch based on the deviation rate from the theoretical value.
[0007] In a further embodiment of the present invention, step S1 comprises the following steps: Anti-pollution sensors with shark skin bionic structures are arranged on the upper layer of the fermentation material; Obtain the thickness of the mucus layer of the fermented material and use the microwave resonance principle to detect the moisture content of the material; The total amount of heat generated by biological metabolism is calculated in combination with the deadweight pressure data of the fermentation materials, and an array of flexible thin film thermistors is arranged at the bottom of the fermentation materials.
[0008] In a further embodiment of the present invention, step S2 comprises the following steps: In the first stage of rapid heating, the temperature is raised to the first critical value at a preset rate, and the over-limit protection threshold of the heating curve is adjusted according to the real-time heat generation; In the second maintenance stage, the heating rate is dynamically adjusted in combination with the thickening rate of the mucus layer of the material, and the duration of this stage is extended when the heat generation fluctuation exceeds the preset range; In the third convergence stage, it is determined whether the stability of the heat production increment meets the constant temperature conditions, and the metabolic activity ratio of the bacterial community is regulated by a multi-frequency pulse cooling mode.
[0009] In a further embodiment of the present invention, step S2 further comprises the following steps: After the bacterial flora enters the mature and stable stage, the alternating temperature control program is started. When the thickness monitoring value S of the middle mucus layer continues to rise, the cooling duration is adjusted to meet the following formula: in, Indicates the duration of the cooling phase, It indicates the maximum thickening of the middle mucus layer measured within 6 hours. The bacterial flora enters a mature and stable stage. The multi-frequency pulse cooling mode is used to change the enzyme activity ratio of the bacterial flora through alternating high and low temperatures to promote the accumulation of secondary metabolites.
[0010] In a further embodiment of the present invention, step S3 comprises the following steps: Multiple sets of magnetic field adjustment devices are embedded in the side wall of the material box to change the current direction of adjacent coils to generate staggered magnetic lines of force that penetrate the material; staggered magnetic lines of force are areas where the magnetic paths generated by adjacent coils driven by currents in different directions intersect and cover each other; Based on the real-time magnetic induction intensity distribution data, the surface fermentation bacteria are guided to the high magnetic field area in the upper middle part of the material, and the bottom heat-producing bacteria are gathered in the low magnetic field area at the bottom.
[0011] In a further embodiment of the present invention, step S4 comprises the following steps: When the moisture content of the monitored middle layer exceeds the moisture content threshold, the directional water absorption program of the porous moisture-conducting membrane is started and the separation recovery device is triggered to collect the aromatic substances in the overflowed liquid; A unidirectional flow path driven by humidity gradient is established through a moisture-conducting membrane with an asymmetric pore structure; The terpene volatile substances in the diversion liquid are separated and recovered by using the condensation temperature difference multi-stage adsorption technology.
[0012] In a further embodiment of the present invention, step S5 comprises the following steps: When the rate of increase of ambient humidity exceeds the threshold within the specified time, the aroma capture particles wrapped in tangerine peel extract are automatically released; the emergency heat dissipation duct is activated, and the air flow direction is automatically changed according to the real-time collected temperature difference data of the upper, middle and lower layers; The inner wall of the emergency heat dissipation duct is coated with a hydrophobic coating formed by polytetrafluoroethylene microspheres, and the surface of the coating has an array of micron-scale protrusions; the temperature difference data specifically refers to the temperature gradient value of adjacent temperature measuring points in the vertical direction of the fermentation material.
[0013] In a further embodiment of the present invention, step S6 comprises the following steps: The limonene, Terpinene content distribution map; The deviation rate between the actual yield of the target component and the system predicted value is recorded as K; when the deviation rate K of multiple consecutive batches exceeds the allowable range, the multivariate linear regression model is used to update the joint control parameter combination of temperature, magnetic field and moisture.
[0014] In a further embodiment of the present invention, the deviation rate between the actual yield of the target component and the system predicted value is denoted as K, which satisfies the following formula: in, Indicates the actual substance content value determined by gas chromatography, measured in mass percentage; Indicates the theoretical yield value preset in the fermentation control system. The value represents the degree of deviation between the current batch production process and the ideal model.
[0015] In a second aspect, the present invention provides a fermentation temperature control system, which adopts the following technical solution: A fermentation temperature control system, comprising the following modules: The data acquisition module is used to obtain the anti-fouling treatment data of the material, detect the moisture content of the mucus layer in the material, and calculate the total heat generated by biological metabolism based on the material's deadweight pressure; The temperature control module triggers the graded temperature control program according to the total amount of heat produced by biological metabolism, and adjusts the temperature distribution of the fermentation pile through the coupling control of the three-stage heating and dynamic maintenance stages; The staggered magnetic field module generates a staggered magnetic field according to the temperature gradient distribution inside the material, guiding the surface bacteria to migrate to the middle and upper high magnetic field area, inhibiting the aggregation concentration of the bottom heat-producing bacteria; A separation and recovery module is used to detect that the moisture content of the middle layer exceeds a threshold value, activate the directional water absorption program of the moisture-conducting membrane and separate and recover the aromatic substances in the overflowed liquid; Directional heat dissipation module releases aroma capture particles according to the sudden change data of ambient humidity, and simultaneously opens the directional heat dissipation duct to balance the temperature difference of the material layer; The process optimization module is used to analyze the actual yield of the target component in the finished product and optimize the initial control parameters of the next batch based on the deviation rate from the theoretical value.
[0016] In summary, the present invention includes the following beneficial technical effects: 1. Through the integration of bionic sensor array and microwave moisture content detection technology, the thickening rate of the mucus layer in the fermentation pile and the material self-weight pressure distribution are obtained in real time, and the total heat production of biological metabolism is estimated by combining the dynamic heat production model. After triggering the three-stage heating program with an over-limit protection mechanism, the heating rate and stage duration are automatically adjusted according to the real-time heat production fluctuations, and the metabolic activity ratio of the bacterial community is regulated through the multi-frequency pulse cooling mode to construct a dynamic temperature gradient that meets the growth characteristics of the bacterial community, effectively solving the problem of uneven distribution of metabolites caused by traditional constant temperature fermentation; 2. Use the pre-buried coil to generate an interlaced magnetic field formed by currents in different directions, and establish an intelligent partitioning system of high and low magnetic induction intensity inside the material. Based on the real-time magnetic induction intensity distribution data, the upper and middle magnetic fields are strengthened to promote the directional migration of surface bacteria to the dominant metabolic area, and the bottom magnetic field is simultaneously weakened to inhibit the aggregation of heat-producing bacteria; 3. By obtaining the actual yield of terpenes in the finished product, a mathematical model of the deviation rate from the theoretical predicted value is established, and the temperature-magnetic field-moisture joint control parameter combination is associated to generate a multivariate regression correction factor. When the deviation rate of consecutive batches exceeds the allowable range, the system iteratively updates the initial control parameters using the least squares method to form a closed loop of process self-optimization. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. The drawings are used to provide a further understanding of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.
[0018] Figure 1 A schematic flow chart of a fermentation temperature control method is disclosed.
[0019] Figure 2 A structural schematic diagram of a fermentation temperature control system is disclosed. DETAILED DESCRIPTION
[0020] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention.
[0021] The following is combined with Figure 1-Figure 2 The preferred embodiments of the present invention are described in detail.
[0022] Refer to the attached Figure 1 The present invention provides a fermentation temperature control method, comprising the following steps: S1. Obtaining the anti-pollution treatment data of the material, including arranging an anti-pollution sensor with a bionic groove structure, detecting the moisture content of the mucus layer in the material, and calculating the total amount of heat generated by biological metabolism in combination with the deadweight pressure of the material; S2. Triggering the hierarchical temperature control program according to the total amount of heat produced by biological metabolism, adjusting the temperature distribution of the fermentation pile through the coupling control of the three-stage heating and dynamic maintenance stages; S3, generating a staggered magnetic field according to the temperature gradient distribution inside the material, guiding the surface bacteria to migrate to the middle and upper high magnetic field area, and inhibiting the aggregation concentration of the bottom heat-producing bacteria; S4, when it is detected that the moisture content of the middle layer exceeds the threshold, the directional water absorption program of the moisture-conducting membrane is activated and the aromatic substances in the overflowed liquid are separated and recovered; S5. Release the aroma capture particles according to the sudden change data of the ambient humidity, and simultaneously open the directional heat dissipation duct to balance the temperature difference of the material layer; S6. Analyze the actual yield of the target component in the finished product and optimize the initial control parameters for the next batch based on the deviation rate from the theoretical value.
[0023] In one embodiment of the present invention, step S1 includes the following steps: S11, an anti-pollution sensor with a shark skin bionic structure is arranged at a depth of 1 cm above the fermentation material. The sensor uses a 20 Hz high-frequency micro-vibration to vibrate the mycelium attachments to an isolation area with a radius of 5 cm around the sensor; Specifically, an anti-pollution sensor with a shark skin bionic structure is deployed at a depth of 1 cm above the fermentation material. The surface of the anti-pollution sensor adopts 3D printing technology to form a gradient groove structure from wide to narrow, and the widest part is gradually reduced from 50 microns to 5 microns. The sensor has an internal integrated piezoelectric vibration module, which generates high-frequency micro-vibrations with an amplitude of 5 microns and a frequency of 20 Hz every 5 seconds. The high-frequency micro-vibration is transmitted to the groove area on the surface of the sensor, and the mycelium and metabolic secretions attached to the surface of the anti-pollution sensor are shaken off to a circular isolation area with a radius of 5 cm. The isolation area is paved with a thin film of hydrophobic material to form a physical barrier to avoid the re-attachment of detached pollutants as much as possible and ensure the stability of the detection signal; The anti-pollution sensor is embedded with an array of temperature-sensitive units, with 36 micro-thermocouple probes arranged per square centimeter. When working continuously, the anti-pollution sensor performs a self-cleaning action every 15 minutes to shake off the mycelium clusters attached to the surface.
[0024] Among them, the shark skin bionic structure is a special surface engineering technology that imitates the diamond-shaped scales and grooves on the surface of shark skin. Its microstructure can destroy the balance between the contact area and surface tension required for microbial attachment. Piezoelectric vibration module is a transducer device that generates mechanical vibration by applying alternating voltage, and its amplitude is precisely controlled at a microscopic scale to avoid interfering with normal bacterial activity.
[0025] Exemplarily, a conventional sensor and an anti-fouling sensor with a shark skin bionic structure were run in the same fermentation environment for 72 hours; The surface of the traditional sensor is covered with a 0.8 mm thick bacterial film, resulting in a temperature detection error of ±2.3°C; the anti-pollution sensor with a shark skin bionic structure has only sporadic attachments on the edge of the sensor, and the maximum detection deviation is controlled within ±0.5°C.
[0026] S12, obtaining the thickness of the mucus layer in the middle layer of the tangerine peel fermentation pile, and when detecting the moisture content of the material using the microwave resonance principle, controlling the transmission power to 0.7 times the ordinary food detection standard to avoid carbonization of the middle mucus layer; Specifically, a microwave resonance probe is installed at a depth of 3cm-5cm in the middle layer of the fermentation material. The microwave resonance probe emits a frequency of The water content is calculated by measuring the frequency offset of the reflected wave using the dielectric loss characteristics of the electromagnetic wave in the water-containing medium.
[0027] When the moisture content of materials is detected using the microwave resonance principle, the microwave transmission power is set to 13 milliwatts per square centimeter, which is 0.7 times the standard for conventional food testing. The microwave generator emits electromagnetic waves to the material layer at a frequency of 1 gigahertz, and the energy attenuation signal passing through the material is captured by the antenna array at the receiving end. Targeted power reduction can avoid the formation of hotspot effects in materials containing a middle mucus layer by high-frequency electromagnetic waves, and minimize the carbonization of sugar substances.
[0028] Among them, the microwave resonance principle is the energy absorption phenomenon caused by the interaction between electromagnetic waves of a specific frequency and polar molecules in the substance. The degree of signal attenuation is positively correlated with the water content. The hotspot effect refers to the local high temperature phenomenon caused by energy concentration when electromagnetic waves propagate in an inhomogeneous medium.
[0029] S13, calculating the total amount of heat produced by biological metabolism based on the self-weight pressure data of the fermentation materials; Specifically, a 6×6 array of flexible thin film thermistors is arranged at the bottom of the fermentation material. The size of a single sensor unit is 8 mm×8 mm, and the temperature measurement accuracy is ±0.05°C. An additional micro pressure sensor is integrated in the center area of the array, with a pressure area of 64 square millimeters and a range of 0-50 kPa. The total amount of heat generated by biological metabolism is calculated to meet the following formula: Specifically, represents the total amount of heat produced by biological metabolism, Indicates the zone temperature value, represents the corresponding orientation weight factor, Indicates the thermal conductivity correction coefficient of the material, Indicates the density of the fermentation material, Represents specific heat capacity.
[0030] For example, when the array center temperature is set to 42.3°C and the edge temperature is set to 40.1°C, and the pressure value P is 12 kPa, the preset tangerine peel fermentation material parameters ρ=385 kg / m3 and Cp=1.76 kJ / (kg·°C) are substituted, and the total heat production of biological metabolism is calculated to be 58.7 watts per square meter, triggering the magnetic field adjustment device to enhance the heat dissipation efficiency of the bottom layer.
[0031] In one embodiment of the present invention, step S2 comprises the following steps: When the bacterial colony enters the rapid reproduction stage, a three-level progressive temperature control program is implemented; The first stage of rapid heating lasts for 2 hours, with the initial control target being 42°C. When the actual temperature reaches 40°C, the over-limit protection program is activated, forcibly limiting the heating rate to 1.2°C per hour.
[0032] The second maintenance stage lasts for 4 hours. In this stage, the temperature rise rate is adjusted dynamically based on the changes in the mycelial mucus layer. The hourly temperature rise rate is adjusted to , and at the same time introduce the temperature balance index of the inner layer of the material to meet the following formula: in, It indicates the relative change of heat generation per unit time. Indicates the average heat generation during the current detection period. Indicates the heat generation baseline value of the previous detection cycle; if Increase by more than one hour When the second level maintenance phase is automatically extended, the maximum allowed extension time is the original setting time. ; For example, setting , , calculated ; If the threshold exceeds 5%, the duration of the second stage will be extended from the preset 4 hours to 6 hours.
[0033] In the third convergence stage, it is continuously determined whether the fluctuation amplitude of the heat generation increment is less than 0.8°C for three consecutive sampling cycles. Once this condition is met, the constant temperature holding mode is immediately switched.
[0034] The gradient temperature rise mode during the explosive growth period is a phased and progressive environmental stimulation method. It promotes the rapid formation of dominant colonies by gradually increasing the metabolic load, and at the same time sets a dynamic delay mechanism to prevent overheating damage.
[0035] After the bacterial flora enters the mature and stable stage, the alternating temperature control program is started. The temperature control sequence consists of a 45°C constant temperature section connected to a 38°C pulse cooling section. When the thickness monitoring value S of the middle mucus layer continues to rise, the cooling duration is adjusted to meet the following formula: in, Indicates the duration of the cooling phase, It indicates the maximum thickening of the middle mucus layer measured within 6 hours. When the bacterial flora enters the mature and stable stage, the multi-frequency pulse cooling mode is used to change the enzyme activity ratio of the bacterial flora through alternating high and low temperatures, thereby promoting the accumulation of secondary metabolites.
[0036] For example, the mucus layer increases from 3.2 mm to 3.7 mm within 6 hours. ; Calculated , select the nearest integer value to perform a cold shock of 8.5 minutes.
[0037] The beneficial effects of this verification example: the physiological characteristics of microorganisms at different growth stages, strengthening the competitiveness of dominant strains through gradient temperature increase, and using pulse cooling to break the metabolic balance and force product synthesis; establishing a parameter adjustment mechanism linked to the metabolic intensity of the flora and the state of by-product accumulation, to avoid the problem of flora degradation that is easily caused by traditional constant temperature cultivation.
[0038] In one embodiment of the present invention, step S3 includes the following steps: S31, six sets of magnetic field adjustment devices are embedded in the side wall of the material box, which generate interlaced magnetic lines of force penetrating the material by changing the current direction of adjacent coils; Specifically, six sets of magnetic field regulating devices with independent control capabilities need to be embedded inside the side wall of the fermentation material containing box. The magnetic field regulating device specifically refers to a multi-turn ring structure formed by winding a wire, which generates a cross-magnetic field line distribution by inputting currents in different directions to two adjacent sets of coils; The current directions of adjacent coils are set as follows: the first and second groups use clockwise winding current, the third and fourth groups switch to counterclockwise winding current, and the fifth and sixth groups resume clockwise winding current, thereby forming three alternating magnetic concentration areas and dispersion areas inside the material; the staggered magnetic lines of force penetrating the material refers to the cross-penetration of magnetic lines of force, so that the magnetic induction intensity from the middle layer to the upper part of the fermentation material is maintained in the range of 200-300 Gauss, and the magnetic induction intensity of the bottom area of the fermentation material is reduced to the range of 50-80 Gauss.
[0039] Among them, the staggered magnetic lines of force are the areas where the magnetic paths generated by adjacent coils driven by currents in different directions intersect and cover each other.
[0040] For example, two groups of adjacent coils are selected: the first group of coils applies a clockwise current of 15A, and the second group of coils applies a counterclockwise current of 12A. At this time, the magnetic induction intensity at the center point of the cross-magnetic region formed between the two groups of coils satisfies the following formula: in, It indicates the magnetic induction intensity at the center of the cross magnetic field between two sets of coils; represents the magnetic permeability of vacuum; Indicates the number of turns of the first set of coils; Indicates the first set of coil currents; Indicates the center distance of the first set of coils; Indicates the number of turns of the second set of coils; Represents the second set of coil current; Indicates the center distance of the second set of coils.
[0041] Preset , , , , , ; Calculate the magnetic induction intensity at the center of the cross-magnetic area between the two sets of coils It was verified that under this parameter, the migration rate of the middle-layer bacterial flora in a single batch of fermentation materials to the magnetic concentration area reached 82%.
[0042] S32, guiding the surface fermentation bacteria to the high magnetic field area in the upper part of the material, and causing the bottom heat-producing bacteria to gather in the low magnetic field area at the bottom; Specifically, based on the real-time magnetic induction intensity data received by the embedded controller, the current value of each coil is automatically adjusted to achieve directional migration of the two types of bacterial colonies. For the bacterial colonies on the surface of the fermentation material whose metabolic efficiency needs to be improved, they are guided to gather in the magnetic concentration area located above the fermentation material. This area generates a superimposed magnetic field in the same direction through two adjacent groups of coils, forming a stable field strength with an average magnetic induction intensity of 280 Gauss; For the bottom layer of the fermented material, the over-growth of heat-producing bacteria needs to be suppressed. The magnetic field line divergence area formed by the reverse current between the coil groups is used to keep the bottom area of the fermented material in a weak magnetic field environment below 100 Gauss, forcing the bacteria to maintain their original distribution state.
[0043] Among them, bacterial colony guidance refers specifically to the bio-electromagnetic control technology that changes the location of microbial aggregation by controlling the distribution of the magnetic field; the principle of magnetic induction intensity superposition refers to the rule of adding the total field strength vector generated by two or more magnetic fields at the same spatial position.
[0044] Exemplarily, when the density of the thermogenic bacteria in the bottom layer is detected to exceed a warning value, the intelligent separation program is triggered; The current direction of the fifth and sixth groups of coils was switched from clockwise to counterclockwise to form a reverse magnetic field superposition with the fourth group of coils; the current intensity of the third group of coils was increased from 10A to 18A to enhance the attraction of the magnetic field in the middle and upper layers. The outward diffusion rate of the heat-producing bacteria in the bottom layer decreased by 67% within 72 hours, and the aggregation density of the target bacteria in the middle and upper layers increased by 51%.
[0045] The beneficial effects of this verification example are: effectively inhibiting the excessive proliferation of harmful bacteria and improving the production efficiency of target metabolites; reducing the risk of equipment failure by replacing traditional mechanical separation devices with magnetic field spatial gradient control; and achieving active intelligent adjustment of bacterial distribution at different fermentation stages, greatly improving process stability.
[0046] In one embodiment of the present invention, step S4 comprises the following steps: When the moisture content of the monitored middle layer exceeds a threshold, the directional water absorption program of the porous moisture-conducting membrane is started and the separate recovery device is triggered to collect the aromatic substances in the overflowed liquid; Specifically, when the moisture content value exceeds the set threshold through the continuous monitoring of the microwave resonance probe pre-buried in the middle layer of the material, the directional moisture conduction program of the porous moisture-conducting membrane is automatically activated. The porous moisture-conducting membrane is a composite fiber material with an asymmetric capillary structure. The hydrophobic side of its surface faces the upper layer of the material, and the hydrophilic side is connected to a hidden water collection tank; when the membrane layer detects the formation of a continuous water film on the contact surface with the material, the lateral micropump is started to establish a pressure gradient to guide the excess water to the low humidity area along the preset path, avoiding local over-drying caused by traditional dehumidification. For the transferred liquid, the aromatic components it contains are immediately transported to a multi-stage condensation recovery device, which fixes volatile substances on the surface of a special adsorbent through temperature difference impact separation technology. The one-way valve group ensures that the liquid flow path is irreversible to prevent the secondary reflux of residual water from interfering with the fermentation environment.
[0047] Among them, stratified water flow is a directional water migration phenomenon caused by the natural humidity difference at different depths of the fermentation material; The moisture content threshold refers to the safe upper limit of moisture in the middle layer of the material determined through multiple tests. Exceeding this value will cause metabolic disorders in the bacterial flora. The porous moisture-conducting membrane refers to a gradient permeability material that imitates the stomata structure of banyan trees. The surface layer is distributed with irregular pores that gradually shrink from 50 microns to 5 microns, and has the ability to conduct moisture selectively in a directional manner. The separate recovery device includes a three-stage condensation unit, which uses the difference in boiling points between aromatic substances and water to achieve phased capture; the directional water absorption program refers to a programmed control sequence that dynamically adjusts the micropump power and diversion path according to the real-time moisture distribution of the material; aromatic substances specifically refer to terpene volatile organic compounds unique to tangerine peel fermentation.
[0048] For example, in the verification implementation of a tangerine peel processing plant, when the microwave resonance probe in the middle layer of the material detects that the moisture content reaches 46% (the critical threshold is 42%), the moisture-conducting membrane automatically turns on the high-speed diversion mode for 5 minutes; the micropump transfers 2.8L of water to the edge drying area at a negative pressure of 35kPa, during which the pore shrinkage rate of the moisture-conducting membrane automatically matches the humidity gradient; the transferred liquid enters the recovery device, and the recovery device The cyclodextrin coating captured 91% of Terpinene component.
[0049] The beneficial effects of this verification example are: directional dehumidification is achieved by identifying abnormal body fluid distribution, getting rid of the high energy consumption defects of traditional equipment; the unique step-by-step recovery process can remove unnecessary moisture while retaining the aromatic essence components to the greatest extent.
[0050] In one embodiment of the present invention, step S5 comprises the following steps: S51, when the ambient humidity rises by more than 30% within 1 hour, the aroma capture particles wrapped by tangerine peel extract are automatically released; Specifically, when the humidity rises by more than 30% within one hour, the aroma capture particles wrapped in tangerine peel extract are automatically released; when the humidity rises suddenly and causes the surface vapor pressure of the fermentation pile to change, the protective film of tangerine peel extract on the outer layer of the particles dissolves, exposing the aroma capture particles. The cyclic sugar molecules specifically capture the surrounding environment through host-guest inclusion complexes. Small molecule volatiles such as terpinene and limonene.
[0051] Among them, the aroma capture particles are a composite structure prepared by physical embedding method, and the core has honeycomb holes. cyclic sugar molecular framework; The cyclic sugar molecular framework is a ring-shaped macromolecule formed by multiple glucose units connected end to end. The diameter of the internal cavity is about 0.6-0.8 nanometers, which is just the right size to fit the three-dimensional structure of terpenes in the tangerine peel aroma molecules.
[0052] S52, synchronously start the hidden emergency cooling air duct, and automatically turn according to the real-time collected temperature difference data of the upper, middle and lower layers; Specifically, the emergency heat dissipation duct is a spiral guide channel surrounding the side wall of the fermentation box. Its opening position is dynamically adjusted according to the temperature difference data of the upper, middle and lower layers of materials collected in real time. When it is judged that the temperature of the upper layer of materials is higher than that of the middle and lower layers by more than 2°C, the air outlet of the duct automatically turns to the top of the high temperature area, and accelerates local heat dissipation by guiding natural convection in the box. The inner wall of the duct is coated with a hydrophobic coating formed by polytetrafluoroethylene microspheres. The surface of the coating has an array of micron-scale protrusions that can reduce the adhesion of condensed water droplets. Among them, the temperature difference data refers specifically to the temperature gradient value of the adjacent temperature measuring points in the vertical direction of the fermentation material, which is obtained by arranging multiple temperature measuring probes at different depths of the fermentation material. The distance between the probes in each layer is not less than 5 cm to avoid thermal field interference. Hidden emergency heat dissipation duct refers to a ventilation duct that is completely embedded in the interlayer of the outer wall of the equipment. The diversion outlet of the corresponding section is opened by a mechanical slider only when the emergency control mode is triggered, and it remains closed under normal circumstances.
[0053] For example, a tangerine peel processing plant encountered a sudden rainstorm, causing the humidity in the workshop to rise from 55% to 86% within 45 minutes. The system detected that the humidity change rate exceeded the set threshold and immediately released 300g of aroma capture particles from the top of the box, with an average particle size of 80 microns. The sensor detected that the temperature of the upper layer of the material increased abnormally by 0.8℃ within 15 minutes, and the emergency air duct automatically adjusted the outlet direction to the high temperature area to form local forced convection.
[0054] The beneficial effects of this verification example: the rapid release of aroma capture particles effectively reduces the risk of aroma components escaping, while the intelligent directional heat dissipation duct avoids imbalance in bacterial activity caused by local overheating; the combination of the two enables the entire control system to quickly suppress the trend of quality deterioration when facing sudden working conditions, while maintaining the normal progress of microbial metabolic activities, significantly improving the anti-interference ability of the production process and the consistency of product quality; in the actual production environment, even in the event of extreme temperature and humidity fluctuations, the aroma characteristic substances of the fermentation products are still ensured to be completely retained.
[0055] In one embodiment of the present invention, step S6 comprises the following steps: The limonene, Terpinene content distribution map; Specifically, the dried tangerine peel fermentation sample is crushed and placed in the injection port. The flowing carrier gas carries the volatiles through the capillary column coated with the stationary liquid. Different components are separated due to the difference in adsorption force. The flame ionization detector captures limonene, The peak time and area of target substances such as terpinene generate a two-dimensional waveform spectrum. The height of the chromatographic peak corresponds to the concentration of the substance, and the width of the peak base reflects the separation purity.
[0056] Among them, the gas chromatograph is a precision detection instrument based on the volatility differences of substances, and its core includes a gas separation column and a signal detector.
[0057] The deviation rate between the actual yield of the target component and the system predicted value is recorded as K, and the optimization coefficient is generated according to the formula to satisfy the following formula: in, Indicates the actual substance content value determined by gas chromatography, measured in mass percentage; Indicates the theoretical yield value preset in the fermentation control system. The value represents the degree of deviation between the current batch production process and the ideal model.
[0058] when The absolute value of , automatically trigger the parameter self-learning module to update the initial control parameter combination of the next batch; Specifically, the parameter self-learning module integrates an intelligent adjustment unit of the multivariate linear regression algorithm, and its received deviation rate , the duration of the temperature control stage, and the intensity of the magnetic field, and the correction law in the historical data set is fitted by the least squares method; the updated parameter combination includes control variables such as the weighted coefficient of the magnetic field action time and the upper limit of the single temperature rise; Exemplary, 3 consecutive batches are tested Terpinene K value reaches , the parameter self-learning module adjusts the magnetic field strength in the middle layer upward , reduce the temperature rise rate , and then the K value of the two batches was adjusted back to .
[0059] See attached Figure 2 The present invention also proposes a fermentation temperature control system, comprising the following modules: The data acquisition module is used to obtain the anti-fouling treatment data of the material, detect the moisture content of the mucus layer in the material, and calculate the total heat generated by biological metabolism based on the material's deadweight pressure; The temperature control module triggers the graded temperature control program according to the total amount of heat produced by biological metabolism, and adjusts the temperature distribution of the fermentation pile through the coupling control of the three-stage heating and dynamic maintenance stages; The staggered magnetic field module generates a staggered magnetic field according to the temperature gradient distribution inside the material, guiding the surface bacteria to migrate to the middle and upper high magnetic field area, inhibiting the aggregation concentration of the bottom heat-producing bacteria; A separation and recovery module is used to detect that the moisture content of the middle layer exceeds a threshold value, activate the directional water absorption program of the moisture-conducting membrane and separate and recover the aromatic substances in the overflowed liquid; Directional heat dissipation module releases aroma capture particles according to the sudden change data of ambient humidity, and simultaneously opens the directional heat dissipation duct to balance the temperature difference of the material layer; The process optimization module is used to analyze the actual yield of the target component in the finished product and optimize the initial control parameters of the next batch based on the deviation rate from the theoretical value.
[0060] The modules described above may be implemented in whole or in part through software, hardware, or a combination thereof, supporting a processor embedded in or independent of a computer device in hardware form, and also supporting a memory stored in a computer device in software form, so that the processor can call and execute operations corresponding to the modules described above.
[0061] It should be noted that the human information (including but not limited to human device information and personal information, etc.) and data (including but not limited to data used for analysis, stored data and displayed data, etc.) involved in the present invention are all information and data authorized by the human body or fully authorized by all parties. The collection, use and processing of relevant data require relevant legal standards.
[0062] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention is described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. Such modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention, and should all be included in the protection scope of the present invention.
Claims
1. A fermentation temperature control method, characterized in that: The following steps are involved: S1. Obtaining the anti-pollution treatment data of the material, including arranging an anti-pollution sensor with a bionic groove structure, detecting the moisture content of the mucus layer in the material, and calculating the total amount of heat generated by biological metabolism in combination with the deadweight pressure of the material; S2. Triggering the hierarchical temperature control program according to the total amount of heat produced by biological metabolism, adjusting the temperature distribution of the fermentation pile through the coupling control of the three-stage heating and dynamic maintenance stages; S3, generating a staggered magnetic field according to the temperature gradient distribution inside the material, guiding the surface bacteria to migrate to the middle and upper high magnetic field area, and inhibiting the aggregation concentration of the bottom heat-producing bacteria; S4, when it is detected that the moisture content of the middle layer exceeds the threshold, the directional water absorption program of the moisture-conducting membrane is activated and the aromatic substances in the overflowed liquid are separated and recovered; S5. Release the aroma capture particles according to the sudden change data of the ambient humidity, and simultaneously open the directional heat dissipation duct to balance the temperature difference of the material layer; S6. Analyze the actual yield of the target component in the finished product and optimize the initial control parameters for the next batch based on the deviation rate from the theoretical value.
2. A fermentation temperature control method according to claim 1, characterized in that: The step S1 comprises the following steps: Anti-pollution sensors with shark skin bionic structures are arranged on the upper layer of the fermentation material; Obtain the thickness of the mucus layer of the fermented material and use the microwave resonance principle to detect the moisture content of the material; The total amount of heat generated by biological metabolism is calculated in combination with the deadweight pressure data of the fermentation materials, and an array of flexible thin film thermistors is arranged at the bottom of the fermentation materials.
3. A fermentation temperature control method according to claim 1, characterized in that: The step S2 comprises the following steps: In the first stage of rapid heating, the temperature is raised to the first critical value at a preset rate, and the over-limit protection threshold of the heating curve is adjusted according to the real-time heat generation; In the second maintenance stage, the heating rate is dynamically adjusted in combination with the thickening rate of the mucus layer of the material, and the duration of this stage is extended when the heat generation fluctuation exceeds the preset range; In the third convergence stage, it is determined whether the stability of the heat production increment meets the constant temperature conditions, and the metabolic activity ratio of the bacterial community is regulated by a multi-frequency pulse cooling mode.
4. A fermentation temperature control method according to claim 3, characterized in that: The step S2 further comprises the following steps: After the bacterial flora enters the mature and stable stage, the alternating temperature control program is started. When the thickness monitoring value S of the middle mucus layer continues to rise, the cooling duration is adjusted to meet the following formula: in, Indicates the duration of the cooling phase, It indicates the maximum thickening of the middle mucus layer measured within 6 hours. The bacterial flora enters a mature and stable stage. The multi-frequency pulse cooling mode is used to change the enzyme activity ratio of the bacterial flora through alternating high and low temperatures to promote the accumulation of secondary metabolites.
5. A fermentation temperature control method according to claim 4, characterized in that: The step S3 comprises the following steps: Multiple sets of magnetic field adjustment devices are embedded in the side wall of the material box to change the current direction of adjacent coils to generate staggered magnetic lines of force that penetrate the material; staggered magnetic lines of force are areas where the magnetic paths generated by adjacent coils driven by currents in different directions intersect and cover each other; Based on the real-time magnetic induction intensity distribution data, the surface fermentation bacteria are guided to the high magnetic field area in the upper middle part of the material, and the bottom heat-producing bacteria are gathered in the low magnetic field area at the bottom.
6. A fermentation temperature control method according to claim 5, characterized in that: The step S4 comprises the following steps: When the moisture content of the monitored middle layer exceeds the moisture content threshold, the directional water absorption program of the porous moisture-conducting membrane is started and the separation recovery device is triggered to collect the aromatic substances in the overflowed liquid; A unidirectional flow path driven by humidity gradient is established through a moisture-conducting membrane with an asymmetric pore structure; The terpene volatile substances in the diversion liquid are separated and recovered by using the condensation temperature difference multi-stage adsorption technology.
7. A fermentation temperature control method according to claim 6, characterized in that: The step S5 comprises the following steps: When the rate of increase of ambient humidity exceeds the threshold within the specified time, the aroma capture particles wrapped in tangerine peel extract are automatically released; the emergency heat dissipation duct is activated, and the air flow direction is automatically changed according to the real-time collected temperature difference data of the upper, middle and lower layers; The inner wall of the emergency heat dissipation duct is coated with a hydrophobic coating formed by polytetrafluoroethylene microspheres, and the surface of the coating has an array of micron-scale protrusions; the temperature difference data specifically refers to the temperature gradient value of adjacent temperature measuring points in the vertical direction of the fermentation material.
8. A fermentation temperature control method according to claim 7, characterized in that: The step S6 comprises the following steps: The limonene, Terpinene content distribution map; The deviation rate between the actual yield of the target component and the system predicted value is recorded as K; when the deviation rate K of multiple consecutive batches exceeds the allowable range, the multivariate linear regression model is used to update the joint control parameter combination of temperature, magnetic field and moisture.
9. A fermentation temperature control method according to claim 8, characterized in that: The deviation rate between the actual yield of the target component and the system predicted value is recorded as K, which satisfies the following formula: in, Indicates the actual substance content value determined by gas chromatography, measured in mass percentage; Indicates the theoretical yield value preset in the fermentation control system. The value represents the degree of deviation between the current batch production process and the ideal model.
10. A fermentation temperature control system, characterized in that: Includes the following modules: The data acquisition module is used to obtain the anti-fouling treatment data of the material, detect the moisture content of the mucus layer in the material, and calculate the total heat generated by biological metabolism based on the material's deadweight pressure; The temperature control module triggers the graded temperature control program according to the total amount of heat produced by biological metabolism, and adjusts the temperature distribution of the fermentation pile through the coupling control of the three-stage heating and dynamic maintenance stages; The staggered magnetic field module generates a staggered magnetic field according to the temperature gradient distribution inside the material, guiding the surface bacteria to migrate to the middle and upper high magnetic field area, inhibiting the aggregation concentration of the bottom heat-producing bacteria; A separation and recovery module is used to detect that the moisture content of the middle layer exceeds a threshold value, activate the directional water absorption program of the moisture-conducting membrane and separate and recover the aromatic substances in the overflowed liquid; Directional heat dissipation module releases aroma capture particles according to the sudden change data of ambient humidity, and simultaneously opens the directional heat dissipation duct to balance the temperature difference of the material layer; The process optimization module is used to analyze the actual yield of the target component in the finished product and optimize the initial control parameters of the next batch based on the deviation rate from the theoretical value.
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