Spider venom extraction device

By designing a spider venom extraction device that includes electrical stimulation and cryopreservation functions, the complex and unsafe problems of spider venom extraction in the prior art are solved, and efficient and safe venom extraction and preservation are achieved in single-person operation.

CN119867023AInactive Publication Date: 2025-04-25DALI UNIV

Patent Information

Application Number
CN202510158841.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-02-13
Publication Date
2025-04-25
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The prior art is difficult to effectively and safely extract the venom of the original spider, and the operation is complicated and requires multiple people to cooperate.

Method used

A spider venom extraction device including abutment, centrifuge tube rack, quick-freezing box, pole, connecting seat, lighting pen and fixed tweezing forceps were designed to extract venom by electrically stimulating the spider and freezing box for freezing and storage using dry ice and quick-freezing box.

Benefits of technology

It realizes the stable extraction and storage of spider venom by a single operation, reducing operation difficulty and human resource requirements, and improving extraction efficiency and safety.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a spider venom extracting device, and belongs to the technical field of spider venom extraction.The spider venom extracting device comprises a base table, a centrifugal tube frame, a quick-freezing box, a vertical rod, a connecting base, an illumination electroprobe and fixing tweezers, when spider venom is extracted, spiders are placed in a tweezers body, and opening and closing of the tweezers body are adjusted through a clamping limiting mechanism; the illuminating electroprobe is held by hand to enable the metal wire to be in contact with a spider, the illuminating lamp switch is pressed, the spider can be conveniently observed by means of the illuminating lamp, the metal wire current switch is pressed, so that current circulation is formed among the base station, the illuminating electroprobe, the spider, the fixing tweezers, the connecting seat and the base station, the spider is electrically stimulated, venom is generated by the spider, and the spider can be conveniently observed. The spider venom is collected by means of an external pipette, and when current circulation is formed among the base station, the lighting electroprobe, the spider, the fixing tweezers, the connecting seat and the base station, the power-on prompting lamp is turned on.
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Description

Technical Field

[0001] The invention relates to the technical field of spider venom extraction, and in particular to a spider venom extraction device. Background Art

[0002] For spiders in the order Araneae, the connection between their venom glands and chelicerae is more complicated, and the venom discharge process does not rely on simple muscle compression, but is more controlled by nerves. This sophisticated venom discharge mechanism makes the conventional bayonet-type muscle compression method of extracting venom ineffective, and it is difficult to achieve stable and effective venom extraction. In addition, the venom discharge mechanism of spiders in the order Araneae is usually accompanied by specific predation and defense behaviors, which further increases the uncertainty in the venom collection process. Therefore, new methods need to be developed for the venom collection of spiders in the order Araneae to ensure the safety and stability of venom collection.

[0003] Most of the existing methods are complicated and have low yields, and require multiple people to operate together. At least one person is required to use tweezers to clamp the spider and fix the spider's limbs, and another person is required to stimulate the spider with electric current to extract venom from the chelicerae and fangs. In addition, the operators need to have certain professional knowledge. Extracting venom from spiders is not a simple task. For example, the Hainan bird spider raised in the animal room is more than ten centimeters long. Generally, it takes three people to cooperate to complete the extraction of venom, and they need to be armed from hand to head. In addition, when extracting spider venom, it is urgent to design a spider venom extraction device to solve the above problems. Summary of the invention

[0004] The technical problem to be solved by the present invention is to design a spider venom extraction device which is simple, efficient, less harmful to spiders and safer for operators.

[0005] The technical solution adopted by the present invention to solve the technical problem is: a spider venom extraction device, including: a base, a centrifuge tube rack, a quick freezing box, a vertical rod, a connecting seat, an illuminating electric pen and fixed forceps;

[0006] The centrifuge tube rack and the quick-freezing box are both arranged on the base, and dry ice is placed at the bottom of the quick-freezing box;

[0007] The base is provided with a placement groove, a damping shaft is provided in the placement groove, and one end of the vertical rod is sleeved outside the damping shaft;

[0008] The illuminating electric pen comprises a pen holder, a pen holder electrical connector, a metal wire and a lighting lamp, wherein the pen holder electrical connector is installed at one end of the pen holder, the metal wire is installed at the other end of the pen holder, the lighting lamp is detachably installed at the end of the pen holder where the metal wire is installed, the pen holder is provided with a metal wire current switch, the lighting lamp is provided with a lighting lamp switch, the lighting lamp has a built-in battery, the pen holder electrical connector is electrically connected to the metal wire current switch, the metal wire current switch is electrically connected to the metal wire for controlling whether the metal wire transmits current, the lighting lamp switch is electrically connected to the lighting lamp for controlling the switch of the lighting lamp, the pen holder is provided with a power-on indication lamp, and the power-on indication lamp is electrically connected to the pen holder electrical connector and the metal wire respectively;

[0009] The fixed tweezers include a tweezer body, a connecting part, a rotating shaft and a clamping and limiting mechanism, wherein the clamping and limiting mechanism is arranged on the tweezer body to control the opening and closing degree of the tweezer body, the tweezer body is connected to the connecting part through the rotating shaft, one end of the tweezer body is provided with a metal head facing the center, the metal head and the connecting part can flow current, and the other end of the tweezer body is provided with a friction head facing the center;

[0010] The connecting seat is detachably mounted on the vertical pole, the connecting portion is detachably connected to the connecting seat, and current can flow between the connecting portion and the connecting seat;

[0011] A positive wire and a negative wire are installed on the base, the positive wire is detachably connected to the pen holder electrical connector and current can flow through it, and the negative wire is detachably connected to the connection seat and current can flow through it;

[0012] When extracting spider venom, the spider is placed in the tweezers body and the opening and closing of the tweezers body is adjusted by the clamping limit mechanism, the illuminating electric pen is held to make the metal wire contact with the spider, the illuminating light switch is pressed, and the spider is conveniently observed with the help of the illuminating light, and the metal wire current switch is pressed to form a current flow between the base, the illuminating electric pen, the spider, the fixed tweezers, the connecting seat and the base, so as to electrically stimulate the spider and make the spider produce venom, and the spider venom is collected with the help of an external pipette, and when the current flow is formed between the base, the illuminating electric pen, the spider, the fixed tweezers, the connecting seat and the base, the power-on indicator light is turned on.

[0013] As a preferred technical solution of the present invention, the connecting seat includes a seat body, a seat body electrical connector is provided at one end of the seat body, the negative pole wire is detachably connected to the seat body electrical connector and current can flow, a screw groove is provided at the other end of the seat body, a through opening is provided in the middle of the seat body, an insulating ring is provided on the inner periphery of the through opening, and the seat body can be detachably installed on the vertical pole through the through opening.

[0014] As a preferred technical solution of the present invention, the connecting part includes a body, the tweezers body is connected to the body through a rotating shaft, the metal head and the body can flow current, a screw is provided on the side opposite to the rotating shaft, the screw is threadedly connected to the screw groove, and when the screw is threadedly connected to the screw groove, the body can flow current through the seat body and the seat body electrical connector.

[0015] As a preferred technical solution of the present invention, the clamping and limiting mechanism includes a limiting ring, a limiting bolt is threadedly connected to the limiting ring, the limiting ring is sleeved on the tweezers body, and the end of the limiting bolt passes through the limiting ring and abuts against the tweezers body.

[0016] As a preferred technical solution of the present invention, the vertical pole is provided with two limiting fixing mechanisms located above and below the connecting seat, and the limiting fixing mechanisms include a first half-ring fixing ear, a second half-ring fixing ear and a fastening bolt, and the first half-ring fixing ear and the second half-ring fixing ear are spliced ​​with each other and mounted on the vertical pole through fastening bolts.

[0017] As a preferred technical solution of the present invention, a partition is provided between the centrifuge tube rack and the quick-freezing box.

[0018] As a preferred technical solution of the present invention, the centrifuge tube rack is provided with a plurality of centrifuge tube placement holes, and centrifuge tubes are placed in the centrifuge tube placement holes.

[0019] As a preferred technical solution of the present invention, the quick freezing box includes a placement portion, and a plurality of freezing placement holes are opened on the placement portion.

[0020] According to a preferred technical solution of the present invention, the quick freezing box also includes a dry ice part, the dry ice part is located below the placement part, an insertion slot with an opening is provided in the dry ice part, a dry ice box is inserted into the insertion slot, a hinge door is provided at the opening position of the insertion slot, and the dry ice box is used to place dry ice.

[0021] As a preferred technical solution of the present invention, a tool box is arranged on the base platform.

[0022] The beneficial effects of the present invention are embodied in:

[0023] 1. Fixed tweezers are provided for clamping spiders. A clamping limit mechanism is provided so that the clamping limit mechanism can be adjusted according to the type of spider, so that the fixed tweezers can be suitable for different types of spiders. At the same time, the spider can be clamped under the action of the clamping limit mechanism, which can free the hands of the staff. The illuminating electric pen can be provided so that the spider can be better observed under the action of the illuminating lamp. The metal wire can be provided so that an electric current can be formed between the base, the illuminating electric pen, the spider, the fixed tweezers, the connecting seat and the base when the power is on, so as to stimulate the spider for extracting the spider venom. In this process, the staff only needs one hand to Holding the lighting electric pen and the external pipette in the other hand, spider venom can be extracted. By setting a rotating shaft, the tweezers body can be adjusted at multiple angles. By setting a dry refrigerator and a quick-freezing box, in combination with a centrifuge tube, the spider venom can be frozen after the extraction to maintain its activity. After optimization and improvement, the application can be completed independently by reducing the number of operators from multiple people to one person. Spider fixation, position adjustment, multi-angle observation, current control, lighting optimization, precise stimulation, and rapid storage are all integrated on a small platform, which greatly reduces human resources and operational difficulty, allowing undergraduates and even ordinary people to get started.

[0024] 2. By setting a metal head at one end of the tweezers body toward the center, the formation of a current loop is ensured. By setting a friction head at the other end of the tweezers body toward the center, it is beneficial to support the spider on the one hand, and on the other hand, it reduces the impact of fear on some spiders when clamped in mid-air, thereby ensuring stability during spider venom extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a front view schematic diagram of the present invention;

[0026] Figure 2 It is a top view schematic diagram of the base and other parts of the present invention;

[0027] Figure 3 It is a side view schematic diagram of the base and other parts of the present invention;

[0028] Figure 4 It is a partial cross-sectional schematic diagram of the dry refrigerator and quick-freezing box of the present invention;

[0029] Figure 5 It is a schematic diagram of the lighting electric pen and other parts of the present invention;

[0030] Figure 6 It is a schematic diagram of the fixing forceps and other parts of the present invention;

[0031] Figure 7 It is a schematic diagram of the connecting seat and other parts of the present invention;

[0032] Figure 8 It is a schematic diagram of the clamping and limiting mechanism and other parts of the present invention;

[0033] Fig. 9 It is a schematic diagram of the limiting fixing structure and other parts of the present invention.

[0034] In the figure: 1, base; 101, placement slot; 1011, damping shaft; 102, positive wire; 103, negative wire; 104, partition; 105, tool box; 2, centrifuge tube rack; 201, centrifuge tube placement hole; 3, dry ice part; 301, insertion slot; 302, dry ice box; 303, hinge door; 4, placement part; 401, freezing placement hole; 5, vertical pole; 6, connecting seat; 601, seat body; 602, through-hole; 603, insulating ring; 604, seat body electrical connector; 605, screw groove; 7, lighting electric pen; 701, pen holder; 702, pen Rod electrical connector; 703, metal wire; 704, lighting lamp; 705, metal wire current switch; 706, lighting lamp switch; 707, adjustable rheostat; 708, transparent shell; 709, power-on indicator light; 8, fixed tweezers; 801, tweezers body; 802, body; 803, screw; 804, rotating shaft; 805, metal head; 806, limiting collar; 807, limiting bolt; 808, friction head; 9, centrifuge tube; 10, limiting fixing mechanism; 1001, first half ring fixing ear; 1002, second half ring fixing ear; 1003, fastening bolt. DETAILED DESCRIPTION

[0035] The present invention will now be described in further detail with reference to the accompanying drawings.

[0036] Combined with Figure 1-9 As shown, a spider venom extraction device includes a base 1, a placement slot 101, a damping shaft 1011, a positive wire 102, a negative wire 103, a partition 104, a tool box 105, a centrifuge tube rack 2, a centrifuge tube placement hole 201, a dry ice part 3, an insertion slot 301, a dry ice box 302, a hinge door 303, a placement part 4, a freezing placement hole 401, a vertical rod 5, a connecting seat 6, a seat body 601, a through hole 602, an insulating ring 603, a seat body electrical connector 604, a screw groove 605, and an illuminating electric pen 7 , pen body 701, pen body electrical connector 702, metal wire 703, lighting lamp 704, metal wire current switch 705, lighting lamp switch 706, adjustable rheostat 707, transparent shell 708, fixed tweezers 8, tweezers body 801, body 802, screw 803, rotating shaft 804, metal head 805, limiting collar 806, limiting bolt 807, centrifuge tube 9, limiting fixing mechanism 10, first half ring fixing ear 1001, second half ring fixing ear 1002 and fastening bolt 1003.

[0037] Combined with Figure 1-9As shown, a spider venom extraction device comprises: a base 1, a centrifuge tube rack 2, a quick-freezing box, a vertical pole 5, a connecting seat 6, an illuminating electric pen 7 and fixed tweezers 8, wherein the centrifuge tube rack 2 and the quick-freezing box are both arranged on the base 1, dry ice is placed at the bottom of the quick-freezing box, a placement slot 101 is arranged on the base 1, preferably, the placement slot 101 is in a rectangular structure, a damping shaft 1011 is arranged in the placement slot 101, preferably, the damping shaft 1011 is arranged at one end of the vertical pole placement slot 101, and the extension direction of the damping shaft 1011 is perpendicular to the extension direction of the placement slot 101, one end of the vertical pole 5 is sleeved outside the damping shaft 1011, and the illuminating electric pen 7 comprises a pen holder 701, a pen holder electrical connector 702, a metal wire 703 and an illuminating electric pen 704. The pen holder 701 is provided with a light 704, the pen holder electrical connector 702 is installed at one end of the pen holder 701, preferably, the pen holder electrical connector 702 is provided with an adjustable rheostat 707 for controlling the current flow, the metal wire 703 is installed at the other end of the pen holder 701, the light 704 is detachably installed on the end of the pen holder 701 where the metal wire 703 is installed, preferably, the light 704 is arranged above the metal wire 703, and the connection between the metal wire 703 and the pen holder 701 is in a conical structure, the pen holder 701 is detachably provided with a transparent shell 708 for protecting the light 704 and the metal wire 703 when not in use, the pen holder 701 is provided with a metal wire current switch 705, and the light 704 is provided with a light The illuminating lamp 704 has a built-in battery, the pen shaft electrical connector 702 is electrically connected to the metal wire current switch 705, the metal wire current switch 705 is electrically connected to the metal wire 703 for controlling whether the metal wire 703 transmits current, the illuminating lamp switch 706 is electrically connected to the illuminating lamp 704 for controlling the switch of the illuminating lamp 704, preferably, the outer surface of the pen shaft 701 is an insulating material, and the pen shaft 701 is provided with a power-on indicator light 709, and the power-on indicator light 709 is electrically connected to the pen shaft electrical connector 702 and the metal wire 703 respectively, the fixed tweezers 8 include a tweezers body 801, a connecting part, a rotating shaft 804 and a clamping limit mechanism, and the clamping limit mechanism is arranged on the tweezers body 80 1 is used to control the opening and closing degree of the tweezers body 801, the tweezers body 801 is connected to the connecting part through a rotating shaft 804, one end of the tweezers body 801 is provided with a metal head 805 towards the center, preferably, the outer surface of the tweezers body 801 is an insulating material, the metal head 805 and the connecting part can flow current, the other end of the tweezers body 801 is provided with a friction head 808 towards the center, preferably, the friction head 808 is a soft silicone anti-slip cover in the shape of an epidermis grid, the connecting seat 6 is detachably mounted on the vertical pole 5, the connecting part and the connecting seat 6 are detachably connected and the connecting part and the connecting seat 6 can flow current, the positive wire 102 and the negative wire 103 are installed on the base 1,Preferably, a built-in power supply is provided in the base 1, and the positive wire 102 and the negative wire 103 are electrically connected to the built-in power supply, wherein the built-in power supply is detachable so as to be replaced, and can also be rechargeable so as to replenish its power, the positive wire 102 is detachably connected to the pen body electrical connector 702 and current can flow, and the negative wire 103 is detachably connected to the connection seat 6 and current can flow;

[0038] With the help of a built-in power supply, the tweezers body 801 is set, and the spider that needs to be extracted from the spider venom is placed at the end. The clamping limit mechanism is adjusted according to the size of the spider, so that the tweezers body 801 can clamp the spider without the spider falling. The staff adjusts the adjustable rheostat 707 to control the size of the current according to the situation of the spider. Then, the staff holds the lighting pen 7 and the external pipette gun, and turns on the lighting lamp 704 through the lighting switch 706. Then, the lighting pen 7 is held at the spider, so that the metal wire 703 abuts against the spider, and the metal wire current switch 705 is controlled to form a current between the base 1, the lighting pen 7, the spider, the fixed tweezers 8, the connecting seat 6 and the base 1, so as to achieve the purpose of stimulating the spider to produce venom. Then, the produced venom is collected through the external pipette gun. In this process, only one staff member is needed to complete the spider venom extraction work, and it can be adjusted for spiders of different body types. Adaptation, further, can control the damage to the spider so that repeated extraction can be performed later, when current flows between the base 1, the lighting pen 7, the spider, the fixed tweezers 8, the connecting seat 6 and the base 1, the power-on indicator light 709 lights up to indicate that the current is connected, so that it can be determined whether the current loop is formed, and since the lighting lamp 704 has a built-in battery, it is possible to turn on the lighting lamp 704 to observe the spider when the spider venom is not extracted, so as to better extract the spider venom later, and since the lighting lamp 704 can be detachably installed on the pen holder 701, it is convenient to replace the lighting lamp 704, and a metal head 805 is provided at one end of the tweezers body 801 toward the center side to ensure the formation of the current loop, and a friction head 808 is provided at the other end of the tweezers body 801 toward the center side, on the one hand, it is beneficial to support the spider, and on the other hand, it reduces the impact of fear on some spiders when they are clamped in mid-air, thereby ensuring the stability of the spider venom extraction.

[0039] Combined with Figure 1-9As shown, the connecting seat 6 includes a seat body 601, one end of the seat body 601 is provided with a seat body electrical connector 604, the negative electrode wire 103 is detachably connected to the seat body electrical connector 604 and current can flow, the other end of the seat body 601 is provided with a screw groove 605, the middle part of the seat body 601 is provided with a through hole 602, the inner periphery of the through hole 602 is provided with an insulating ring 603, preferably, the insulating ring 603 is made of rubber material, and the hole diameter of the insulating ring 603 is slightly smaller than the diameter of the vertical pole 5, the seat body 601 is detachably installed on the vertical pole 5 through the through hole 602, and the through hole 602 is provided to facilitate the installation of the seat body 601 on the vertical pole 5, and the insulating ring 603 is provided to avoid the current from being transmitted to the vertical pole 5 when the spider venom is extracted, because the insulating ring 603 is made of rubber material, and the hole diameter of the insulating ring 603 is slightly smaller than the diameter of the vertical pole 5, After the rod 5 is put on, friction can be generated between the two, so as to prevent the base 601 from rotating relative to the vertical rod 5 during operation. The connecting part includes a body 802, and the tweezers body 801 is connected to the body 802 through a rotating shaft 804. The metal head 805 and the body 802 can carry current. A screw 803 is provided on the side opposite to the rotating shaft 804 of the body 802, and the screw 803 is threadedly connected to the screw groove 605. When the screw 803 is threadedly connected to the screw groove 605, the body 802 can carry current through the base 601 and the base electrical connector 604. By providing the screw 803 and the screw groove 605, the detachable connection between the body 802 and the base 601 is facilitated. By providing the metal head 805, when the spider is clamped, the spider is clamped by the metal head 805. When current is flowing, it is convenient for current to flow through the spider.

[0040] Combined with Figure 1-9 As shown, the clamping and limiting mechanism includes a limiting ring 806, and a limiting bolt 807 is threadedly connected to the limiting ring 806. The limiting ring 806 is mounted on the tweezers body 801, and the end of the limiting bolt 807 passes through the limiting ring 806 and abuts against the tweezers body 801. By setting the limiting bolt 807, according to the size of the spider to be extracted from the spider venom, after the tweezers body 801 completes the clamping of the spider, the limiting bolt 807 is rotated to adjust the degree of clamping of the spider.

[0041] Combined with Figure 1-9As shown, the vertical pole 5 is provided with two limiting fixing mechanisms 10 located above and below the connecting seat 6, and the limiting fixing mechanism 10 includes a first semi-ring fixing ear 1001, a second semi-ring fixing ear 1002 and a fastening bolt 1003. The first semi-ring fixing ear 1001 and the second semi-ring fixing ear 1002 are spliced ​​and sleeved on the vertical pole 5 by the fastening bolt 1003. By providing the limiting fixing mechanism 10, after the installation of the connecting seat 6 is completed, the position of the limiting fixing mechanism 10 is adjusted, and then the fastening bolt 1003 is rotated to fix the position of the first semi-ring fixing ear 1001 and the second semi-ring fixing ear 1002 to support the connecting seat 6, thereby preventing the connecting seat 6 from falling off when the spider venom is extracted. Since the limiting fixing mechanism 10 is also provided above the connecting seat 6, the upward displacement of the connecting seat 6 is avoided when an upward force is applied to the connecting seat 6 due to the operation process.

[0042] Combined with Figure 1-9 As shown, a partition 104 is provided between the centrifuge tube rack 2 and the quick-freezing box. By providing the partition 104, the effect of dry ice freezing on the centrifuge tube rack 2 is reduced. Preferably, the partition 104 is made of a polyurethane foam board to ensure the effect of the partition 104. The centrifuge tube rack 2 is provided with a plurality of centrifuge tube placement holes 201. Centrifuge tubes 9 are placed in the centrifuge tube placement holes 201. The centrifuge tubes 9 are provided to collect spider venom extracted by an external pipette. The quick-freezing box includes a placement portion 4. The placement portion 4 is provided with a plurality of freezing placement holes 401. By providing the freezing placement holes 401, after the spider venom is collected with the help of the centrifuge tubes 9, the centrifuge tubes 9 can be transferred to the freezing placement holes 401 in time for placement to ensure the retention of the active ingredients of the spider venom. Preferably, the placement A protective cover is detachably mounted on the placement portion 4 for protecting the placement portion 4 when not in use. The quick freezing box further comprises a dry ice portion 3, which is located below the placement portion 4. An insertion slot 301 having an opening is provided in the dry ice portion 3, and a hinge door 303 is provided at the opening position of the insertion slot 301. The dry ice box 302 is used for placing dry ice. By providing the dry ice box 302, the placement and replacement of dry ice is facilitated. A tool box 105 is provided on the base 1. By providing the tool box 105, when spider venom extraction is not performed, the illuminating electric pen 7, fixing tweezers 8, connecting seat 6 and limiting fixing mechanism 10 are placed. The placement slot 101 and the damping shaft 1011 are cooperated to deflect the vertical rod 5 into the placement slot 101 when spider venom extraction is not performed.

[0043] Working principle: When extracting spider venom, place the spider in the tweezers body 801, and adjust the opening and closing degree of the tweezers body 801 by adjusting the limit bolt 807 to ensure that the spider is firmly clamped and will not be hurt. Hold the lighting pen 7 so that the lighting lamp 704 is turned on and the metal wire 703 is energized. Then, the metal wire 703 is brought into contact with the spider, so that current flows between the base 1, the lighting pen 7, the spider, the fixed tweezers 8, the connecting seat 6 and the base 1 to electrically stimulate the spider and make the spider produce venom. The spider venom is collected with the help of an external pipette. After the collection is completed, take the centrifuge tube 9 to collect the spider venom in the external pipette, and then place it in the quick-freezing placement hole 401 on the placement part 4. The spider venom in the centrifuge tube 9 is frozen to ensure its activity.

[0044] Specifically, when the spider is stimulated by means of the electrified metal wire 703, a variety of current sizes and click times can be used for adjustment. Regarding the selection of current size, in the actual test process, 9 Wuliangshan giant wart spiders of the same size and good growth condition under the same environment were selected, the control variable was the amount of water used to moisten the spider's chelicerae, which was one drop of a standard rubber-tipped dropper, the number of electric shocks was 4 times, each with an interval of 3 seconds, and 2 ml of ether was used for anesthesia for 15 minutes until the spider was obviously lazy. The specific conditions of obtaining venom under different currents in this case are shown in Table 1. Regarding the selection of the number of electric shocks, 6 Wuliangshan giant wart spiders of the same size and good growth condition under the same environment were selected, the control variable was the optimal current obtained by the experiment (with the first gear current in Table 1 as a reference), in the experiment of finding the number of motors when the venom output was the largest, each click was separated by 3 seconds, and in the comparison experiment of the venom output with the old continuous current venom extraction device, the venom was extracted for 21 seconds. The amount of water used to moisten the spider's chelicerae is one drop of a standard rubber-tipped dropper. This is the selection of different numbers of electric shocks, as shown in Table 2. Compared with the traditional method of spider venom extraction, the difference in the time used for spider venom extraction is shown in Table 3. When the first gear current in Table 1 is used and the current stimulation time is 21 seconds, the difference in spider damage is shown in Table 4. The spider venom taken in Table 2 was used for quality inspection and the difference is shown in Table 5.

[0045]

[0046] Table 1 Extraction of spider venom at different current levels

[0047]

[0048]

[0049] Table 2 Extraction of spider venom at different electric shock times

[0050]

[0051] Table 3 Comparison of personnel and time used for spider venom extraction between the present invention and traditional methods

[0052]

[0053] Table 4 Comparison of spider damage between the present invention and traditional spider venom extraction

[0054]

[0055] Table 5 Comparison of spider venom quality between the present invention and traditional spider venom extraction

[0056] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. The above embodiments and the description in the specification are only preferred examples of the present invention and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, which fall within the scope of the present invention to be protected. The scope of the present invention to be protected is defined by the attached claims and their equivalents.

Claims

1. A spider venom extraction device, comprising: The base (1), the centrifuge tube rack (2), the quick freezing box, the vertical pole (5), the connecting seat (6), the lighting electric pen (7) and the fixing forceps (8) are characterized by: The centrifuge tube rack (2) and the quick-freezing box are both arranged on the base (1), and dry ice is placed at the bottom of the quick-freezing box; The base (1) is provided with a placement groove (101), a damping shaft (1011) is provided in the placement groove (101), and one end of the vertical rod (5) is sleeved outside the damping shaft (1011); The illuminating electric pen (7) comprises a pen holder (701), a pen holder electrical connector (702), a metal wire (703) and an illuminating lamp (704); the pen holder electrical connector (702) is mounted on one end of the pen holder (701); the metal wire (703) is mounted on the other end of the pen holder (701); the illuminating lamp (704) is detachably mounted on the end of the pen holder (701) on which the metal wire (703) is mounted; the pen holder (701) is provided with a metal wire current switch (705); the illuminating lamp (704) is provided with an illuminating lamp switch (706); and the illuminating lamp (704) is provided with a metal wire current switch (706). 704) has a built-in battery, the pen body electrical connector (702) is electrically connected to the metal wire current switch (705), the metal wire current switch (705) is electrically connected to the metal wire (703) for controlling whether the metal wire (703) transmits current, the lighting switch (706) is electrically connected to the lighting (704) for controlling the lighting (704) to switch on and off, and the pen body (701) is provided with a power-on indicator light (709), which is electrically connected to the pen body electrical connector (702) and the metal wire (703) respectively; The fixed tweezers (8) comprise a tweezer body (801), a connecting portion, a rotating shaft (804) and a clamping and limiting mechanism, wherein the clamping and limiting mechanism is arranged on the tweezer body (801) and is used to control the opening and closing degree of the tweezer body (801); the tweezer body (801) is connected to the connecting portion via the rotating shaft (804); a metal head (805) is arranged at one end of the tweezer body (801) facing the center, and current can flow between the metal head (805) and the connecting portion; and a friction head (808) is arranged at the other end of the tweezer body (801) facing the center. The connecting seat (6) is detachably mounted on the vertical pole (5), the connecting portion and the connecting seat (6) are detachably connected, and current can flow between the connecting portion and the connecting seat (6); The base (1) is provided with a positive wire (102) and a negative wire (103), wherein the positive wire (102) is detachably connected to the pen holder electrical connector (702) and current can flow therethrough, and the negative wire (103) is detachably connected to the connection base (6) and current can flow therethrough; When extracting spider venom, the spider is placed in the tweezers body (801) and the opening and closing of the tweezers body (801) is adjusted by a clamping limit mechanism. The lighting pen (7) is held to make the metal wire (703) contact the spider. The lighting light switch (706) is pressed to facilitate observation of the spider with the help of the lighting light (704). The metal wire current switch (705) is pressed to form a current flow between the base (1), the lighting pen (7), the spider, the fixed tweezers (8), the connecting seat (6) and the base (1) to electrically stimulate the spider and make the spider produce venom. The spider venom is collected with the help of an external pipette. When the current flows between the base (1), the lighting pen (7), the spider, the fixed tweezers (8), the connecting seat (6) and the base (1), the power-on indicator light (709) lights up.

2. A spider venom extraction device according to claim 1, characterized in that: The connection seat (6) comprises a seat body (601), one end of which is provided with a seat body electrical connector (604), the negative pole wire (103) is detachably connected to the seat body electrical connector (604) and current can flow therethrough, the other end of the seat body (601) is provided with a screw groove (605), the middle of the seat body (601) is provided with a through hole (602), the inner periphery of the through hole (602) is provided with an insulating ring (603), and the seat body (601) is detachably mounted on the vertical pole (5) through the through hole (602).

3. A spider venom extraction device according to claim 2, characterized in that: The connecting part includes a body (802), the tweezers body (801) is connected to the body (802) via a rotating shaft (804), the metal head (805) and the body (802) can carry current, a screw (803) is provided on the side of the body (802) opposite to the rotating shaft (804), the screw (803) is threadedly connected to the screw groove (605), and when the screw (803) is threadedly connected to the screw groove (605), the body (802) can carry current through the seat body (601) and the seat body electrical connector (604).

4. A spider venom extraction device according to claim 3, characterized in that: The clamping and limiting mechanism comprises a limiting ring (806), a limiting bolt (807) is threadedly connected to the limiting ring (806), the limiting ring (806) is sleeved on the tweezers body (801), and the end of the limiting bolt (807) passes through the limiting ring (806) and abuts against the tweezers body (801).

5. The spider venom extraction device according to claim 1, characterized in that: The vertical pole (5) is provided with two limiting fixing mechanisms (10) located above and below the connecting seat (6); the limiting fixing mechanism (10) comprises a first semi-ring fixing ear (1001), a second semi-ring fixing ear (1002) and a fastening bolt (1003); the first semi-ring fixing ear (1001) and the second semi-ring fixing ear (1002) are spliced ​​with each other through the fastening bolt (1003) and are sleeved on the vertical pole (5).

6. The spider venom extraction device according to claim 1, characterized in that: A partition (104) is provided between the centrifuge tube rack (2) and the quick-freezing box.

7. The spider venom extraction device according to claim 1, characterized in that: The centrifuge tube rack (2) is provided with a plurality of centrifuge tube placement holes (201), and centrifuge tubes (9) are placed in the centrifuge tube placement holes (201).

8. The spider venom extraction device according to claim 1, characterized in that: The quick-freezing box comprises a placement portion (4), and a plurality of freezing placement holes (401) are provided on the placement portion (4).

9. The spider venom extraction device according to claim 8, characterized in that: The quick freezing box also includes a dry ice portion (3), the dry ice portion (3) being located below the placement portion (4), an insertion slot (301) having an opening being provided in the dry ice portion (3), a dry ice box (302) being inserted into the insertion slot (301), a hinge door (303) being provided at the opening position of the insertion slot (301), and the dry ice box (302) being used for placing dry ice.

10. The spider venom extraction device according to claim 1, characterized in that: A tool box (105) is arranged on the base platform (1).

Citation Information

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