Use of 1-methoxy-2-propanol as an active ingredient in the preparation of mosquito attractants

Abstract

The application belongs to the technical field of mosquito trapping, and particularly relates to application of 1-methoxy-2-propanol as an active ingredient in preparation of a mosquito trapping agent. The mosquito trapping agent is easy to realize industrialization and is suitable for popularization and use. The mosquito trapping agent 1-methoxy-2-propanol in the application is derived from volatile substances released under natural conditions of humans and mice, can simulate the release mode of host volatile substances, makes up for the deficiency of existing biological control, and has a significant attraction effect.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of mosquito trapping, and particularly relates to application of 1-methoxy-2-propanol as an active ingredient in preparation of a mosquito trapping agent. BACKGROUND

[0002] Mosquito-borne diseases, including malaria, dengue, chikungunya, Zika, West Nile fever, Japanese encephalitis and lymphatic filariasis, are still rampant worldwide and cause millions of deaths every year. In recent years, with the acceleration of global warming, globalization and urbanization, the threat of mosquito-borne diseases has intensified. Although mosquito control is still the main means of preventing and controlling these diseases, traditional physical methods are environmentally friendly but have limited effect, and chemical methods are facing difficulties due to the increasing resistance of mosquitoes. Therefore, it is urgent to develop new mosquito control strategies to meet this challenge.

[0003] Aedes aegypti is originally distributed in Africa and has now expanded to the entire tropics and some temperate regions. It is distributed in Hainan Province, Yunnan Province, Guangdong Province and Guangxi Zhuang Autonomous Region, etc. in China, and is the main vector of urban yellow fever, chikungunya and dengue fever, and is one of the most dangerous mosquito species. In the past 20 years, the number of cases of these diseases has increased rapidly worldwide, becoming an important global public health problem. With the continuous increase of dengue fever cases in Southeast Asian countries, the number of imported dengue fever cases in neighboring countries has also increased, which has caused several local outbreaks of diseases transmitted by Aedes aegypti, challenging the national public health system.

[0004] Culex pipiens pallens is an important vector of West Nile fever, epidemic encephalitis B and filariasis, and is widely distributed in the northern part of China above 32 degrees north latitude.

[0005] Different hosts emit odors with their own characteristics. In order to achieve selective trapping of mosquitoes that prefer different hosts, some specific compounds can be added to more accurately and efficiently trap mosquito vectors.

[0006] 1-methoxy-2-propanol is an important organic compound with good solubility, which can be miscible with water and various organic solvents, so it is often used as a solvent and dispersant. It is also widely used in the preparation of electronic device packaging and insulating materials due to its ability to improve the solubility, flowability and coating properties of epoxy resins. However, its application in the field of mosquito trapping has received little attention and needs to be developed. SUMMARY

[0007] In view of the deficiencies of the prior art, the purpose of the present application is to provide the application of 1-methoxy-2-propanol as an active ingredient in the preparation of a mosquito trapping agent.

[0008] The technical scheme adopted by the present application to solve its technical problems is:

[0009] In a first aspect, the present application protects the use of 1-methoxy-2-propanol as an active ingredient in the preparation of a mosquito attractant.

[0010] In a second aspect, the present application protects the use of 1-methoxy-2-propanol as a synergist in the preparation of a mosquito attractant.

[0011] In specific embodiments, the 1-methoxy-2-propanol has the following structure (I):

[0012]

[0013] Formula (I).

[0014] In specific embodiments, the attractant can also contain other active agents or related active ingredients that attract mosquitoes.

[0015] In specific embodiments, the attractant also contains other carriers or adjuvants.

[0016] In specific embodiments, the mosquitoes are Aedes aegypti and / or Culex pipiens pallens.

[0017] In specific embodiments, the solvent in the mosquito attractant is a volatile solvent.

[0018] In specific embodiments, the solvent is n-hexane.

[0019] In specific embodiments, the concentration of 1-methoxy-2-propanol is 0.05-5 µg / µL, specifically 0.05-1 µg / µL, 0.8-2.5 µg / µL, 1.0-5 µg / µL, etc.

[0020] In more specific embodiments, the concentration of 1-methoxy-2-propanol is 0.1-1 µg / µL, specifically 0.1 µg / µL, 0.5 µg / µL, 0.8 µg / µL, 1 µg / µL, etc.

[0021] In even more specific embodiments, the concentration of 1-methoxy-2-propanol in the mosquito attractant to attract Aedes aegypti is 0.1 µg / µL.

[0022] In more specific embodiments, the concentration of 1-methoxy-2-propanol in the mosquito attractant to attract Culex pipiens pallens is 1 µg / µL.

[0023] Advantages

[0024] The 1-methoxy-2-propanol provided by the application can be used as the main active ingredient of the mosquito attractant, and is screened based on the volatiles of the skin flora of mice, and has the following advantages:

[0025] The mosquito attractant 1-methoxy-2-propanol in the application is derived from the volatiles released by humans and mice under natural conditions, can simulate the release mode of host volatiles, make up for the shortcomings of existing biological control, and has a significant attraction effect. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 For the invention based on Pou2f3 - / - Screening results of volatiles of skin flora of mice.

[0027] Figure 2 For the behavioral screening results of the mosquito attractant 1-methoxy-2-propanol in the application by using a three-cage olfactometer device.

[0028] Figure 3 For the dosage screening and mosquito attraction results of the mosquito attractant 1-methoxy-2-propanol placed in a mosquito killer lamp in the application. DETAILED DESCRIPTION

[0029] The application will be further described in detail below in combination with examples. If the reagents or instrument devices used are not marked with the manufacturer, they are all regarded as conventional products that can be purchased in the market.

[0030] The application provides a use of 1-methoxy-2-propanol as a component of a mosquito attractant, wherein the active ingredient of the mosquito attractant contains 1-methoxy-2-propanol. The active ingredient refers to a component that plays an attracting role, and is relative to auxiliary materials, solvents and other attracting components that do not interact. Each component of the mosquito attractant can be a conventional commercially available product. The use includes a use of 1-methoxy-2-propanol for preparing a mosquito attractant product, which can be used as an attractant or an attracting device; a use of 1-methoxy-2-propanol for a mosquito attractant, which can be directly used, in the form of a composition, or through a volatilization device. In summary, as long as various direct or indirect uses of the function of the mosquito attracting effect of 1-methoxy-2-propanol are used, they all belong to the technical solutions to be protected by the application.

[0031] Example 1: Screening of volatiles of skin flora of strong attractive mice (Pou2f3- / - mice, KO mice)

[0032] The skin odor of the mice with higher attraction to mosquitoes was collected and subjected to flavorome detection by gas chromatography-mass spectrometry. The relative odor activity value (ROAV) of 1-methoxy-2-propanol in the odor of the mice with strong attraction was the highest, and the ROAV value of 1-methoxy-2-propanol in the odor of the mice with strong attraction increased most obviously, indicating that 1-methoxy-2-propanol contributed more to the odor of the mice with strong attraction. The relative content of 1-methoxy-2-propanol in the mice with strong attraction was also significantly higher than that in the wild-type mice. The experimental results are shown in Figure 1 .

[0033] Example 2: Test of the attraction effect of single component 1-methoxy-2-propanol in a three-cage olfactometer device

[0034] 1. Preparation of mosquito attractant

[0035] 1-methoxy-2-propanol stock solution and n-hexane stock solution were purchased. 1-methoxy-2-propanol was used as the solute and n-hexane was used as the solvent to prepare a compound solution of 1 µg / µL.

[0036] 1-methoxy-2-propanol: manufacturer: sigma; item number: 484407; specification: 1 L

[0037] n-hexane: manufacturer: sigma; item number: 270504; specification: 4 L

[0038] 2. Three-cage olfactory attraction experiment

[0039] Three anesthetized wild-type mice were placed on each side of the device, and 10 µL of mosquito attractant solution and 10 µL of n-hexane solution were added, respectively. Sixty Culex pipiens pallens female mosquitoes were placed in the middle cage, and after 30 minutes of adaptation, the gates on both sides were opened, and the number of female mosquitoes on both sides was counted for thirty minutes. The experiment was repeated three times. The experimental results are shown in Figure 2 The average attraction rate of the mice with mosquito attractant solution to mosquitoes was 84.72%, while the average attraction rate of the mice with n-hexane solution to mosquitoes was 15.28%, indicating that the mosquito attractant solution had significant attraction effect on mosquitoes.

[0040] Example 3: Screening of effective working concentration of 1-methoxy-2-propanol applied to mosquito eradication lamp

[0041] 1. Preparation of mosquito attractant

[0042] 1-methoxy-2-propanol stock solution and n-hexane stock solution were purchased. 1-methoxy-2-propanol was used as the solute and n-hexane was used as the solvent to prepare solutions with different concentrations, i.e., 0.01 µg / µL, 0.1 µg / µL, and 1 µg / µL.

[0043] 2. Source of human skin-like volatile liquid: Greenly mosquito attractor box

[0044] 3. Use 1-methoxy-2-propanol to cooperate with human skin-like volatile liquid to conduct attraction experiment

[0045] Process description: A 3m*1.5m*2m space is built with mosquito nets in the room. The cotton balls soaked in various mosquito attractant solutions are put into the mosquito attractor box, and the three mosquito attractors and the mosquito attractor with only the mosquito attractor box are placed in the four corners of the space, and the Aedes aegypti or Culex pipiens pallens is released in the center. Open the mosquito attractor, count the number of mosquitoes in each mosquito attractor after 2 hours. Exchange the position of the mosquito attractor after each experiment to eliminate the influence of space effect.

[0046] The concentration of 1-methoxy-2-propanol acting on Aedes aegypti is 0.1 µg / µL. The relationship between the concentration of 1-methoxy-2-propanol and the attraction effect is obvious, and it has good attraction effect when the concentration is 0.1 µg / µL. The concentration of 1-methoxy-2-propanol acting on Culex pipiens pallens is 1 µg / µL. The relationship between the concentration of 1-methoxy-2-propanol and the attraction effect is obvious, and it has good attraction effect when the concentration is 1 µg / µL. The specific experimental results are shown in Figure 3 .

[0047] The protection content of the present application is not limited to the above examples. Changes and advantages that can be thought of by those skilled in the art without departing from the spirit and scope of the present application are included in the present application, and the appended claims are protected.

Claims

1. Application of 1-methoxy-2-propanol as an active ingredient in the preparation of mosquito attractants.

2. The application according to claim 1, characterized in that, The mosquitoes mentioned are Aedes aegypti and / or Culex pipiens pallens.

3. The application according to claim 2, characterized in that, The solvent in the mosquito attractant is a volatile solvent.

4. The application according to claim 3, characterized in that, The solvent is n-hexane.

5. The application according to claim 1, characterized in that, The concentration of 1-methoxy-2-propanol is 0.05~5µg / µL.

6. The application according to claim 1, characterized in that, The concentration of 1-methoxy-2-propanol is 0.1~1 µg / µL.

7. The application according to claim 6, characterized in that, The concentration of 1-methoxy-2-propanol used by the mosquito attractant to attract Aedes aegypti mosquitoes is 0.1 µg / µL.

8. The application according to claim 6, characterized in that, The concentration of 1-methoxy-2-propanol used by the mosquito attractant to attract Culex pipiens pallens is 1 µg / µL.

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