A pheromone composition for codling moth larvae and use thereof

Abstract

The application discloses a kind of cydia pomella larva's food lure composition and application thereof.It includes the following weight parts of component: isoquercitrin 0.02~0.07 parts, phlorizin 0.6~0.9 parts, cyanidin-3-O-galactoside 0.01~0.02 parts, delphinidin-3-O-galactose 0.05~0.12 parts.The food lure component provided in the application has low dosage and low price, can improve the behavior regulation effect of lure core on larvae, reduce the production cost, and is suitable for popularization and promotion.

Description

Technical fields:

[0001] This invention belongs to the field of pest trapping technology, specifically relating to a baiting agent composition for codling moth larvae and its application. Background technology:

[0002] The codling moth (Cydia pomonella) is one of the most important fruit tree pests worldwide. In China, it is mainly distributed in northern fruit-growing areas such as Xinjiang, Gansu, Ningxia, Hebei, Shanxi, and Shaanxi, with Xinjiang being particularly severely affected. It is also sporadically found in some southern regions (such as Sichuan and Yunnan). Its larvae bore into the fruit, causing holes, rot, or fruit drop, severely impacting commercial value and farmers' income. It also increases the risk of disease transmission, leading to reduced yields or even total crop failure. The codling moth is characterized by overlapping generations and strong flight capabilities, with 2-4 generations per year. It spreads rapidly, is difficult to control, and exhibits strong resistance to pesticides, gradually reducing the effectiveness of traditional chemical control methods.

[0003] The fruit-boring behavior of the codling moth larvae poses a significant challenge to the control of chemical pesticides and biological agents. After hatching, the larvae quickly burrow into the fruit, reducing the chance of direct contact between pesticides and the larvae. Simultaneously, the fruit's peel and internal tissues provide a physical barrier, hindering pesticide penetration and ingestion, particularly limiting the effectiveness of contact pesticides and biological agents that require feeding. Furthermore, the boring behavior enhances the pest's concealment, increasing the difficulty of monitoring and control, often leading to delays in control measures and the spread of the infestation.

[0004] The availability of ideal methods for regulating feeding behavior significantly impacts pest control. Attractants can slow down the borer speed, thus increasing the duration of pesticide action and concentrating insects at the site of direct chemical application. Using attractants can regulate the behavior of codling moth larvae, thereby enhancing control efficacy. Compared to sex pheromones, attractants target more harmful larvae, offering greater selectivity, persistence, and stability, and are easier to use and promote. Furthermore, attractants can be used in conjunction with other pesticides or methods to improve the control of codling moth pests. In actual production, chemical control is often implemented after pests appear, while attractants can control pests before they bore in, reducing the time and number of pests damaging fruit production.

[0005] Therefore, there is a need to provide a lure containing a composition of attractants for codling moth larvae to alleviate the problems of short control window, high cost, difficulty in operation, and difficulty in popularization, and to provide diversified options for the control technology of codling moth. Summary of the Invention:

[0006] The purpose of this invention is to overcome the shortcomings of traditional agronomic trapping techniques, such as the inability to effectively target and control codling moth larvae, high labor costs, and difficulty in widespread adoption. This invention provides a codling moth larvae attractant composition and its application to reduce labor costs, improve pesticide efficacy, and achieve centralized extermination, thereby contributing to both reduction of pesticide use and crop loss and lowering field production costs.

[0007] The first objective of this invention is to provide a feeding attractant composition for codling moth larvae, the feeding attractant composition comprising the following components in parts by weight: 0.02-0.07 parts of isoquercitrin, 0.6-0.9 parts of phlorizin, 0.01-0.02 parts of cyanidin-3-O-galactoside, and 0.05-0.12 parts of delphinidin-3-O-galactose.

[0008] Preferably, the palatability-enhancing composition comprises the following components in parts by weight: 0.056 parts of isoquercitrin, 0.84 parts of phlorizin, 0.016 parts of cyanidin-3-O-galactoside, and 0.084 parts of delphinidin-3-O-galactose.

[0009] A second object of the present invention is to provide the use of the above-described attractant composition for codling moth larvae in attracting and / or controlling codling moths.

[0010] Preferably, the application includes applying the attractant composition of the codling moth larvae to a blank lure to attract the codling moth larvae; or applying the attractant composition of the codling moth larvae to a blank lure and then using the lure in combination with a chemical pesticide / biological pesticide / sticky board.

[0011] A third objective of this invention is to provide a method for controlling codling moth larvae, which involves applying the aforementioned codling moth larvae attractant composition to the habitat of the codling moth to attract the codling moth.

[0012] Preferably, the amount of the attractant composition for codling moth larvae is 1-10 mg / m².

[0013] Compared with the prior art, the advantages of the present invention are:

[0014] (1) Simple and flexible in use. The attractant composition for codling moth larvae provided by this invention has multiple components that can be used together in proportion to regulate larval behavior, or can be used in conjunction with pesticides / sticky boards for concentrated extermination and control, making it more autonomous.

[0015] (2) Compared with traditional sex pheromones and other attractants for adult codling moths, lures containing codling moth larvae attractants have stronger targeting and persistence in the field, significantly reducing the manpower required for control.

[0016] (3) The palatability-inducing ingredients provided by the present invention have low dosage and low price, which can reduce the application cost of behavior regulation technology and is suitable for popularization and promotion. Attached image description:

[0017] Figure 1 These are the test data from Example 1. Detailed implementation method:

[0018] The present invention will be further described in detail below with reference to specific embodiments. The embodiments given are only for illustrating the present invention and are not intended to limit the scope of the present invention.

[0019] Example 1

[0020] From January 5 to 8, 2025, an indoor feeding induction experiment was conducted at the Institute of Plant Protection, Chinese Academy of Agricultural Sciences.

[0021] The components of the attractant—isoquercetin, phlorizin, cyanidin-3-O-galactoside, and delphinidin-3-O-galactose—are all over 99% pure.

[0022] Using a 1:1 volume ratio mixture of ultrapure water and dimethyl sulfoxide as a solvent, solutions of isoquercitrin, phlorizin, cyanidin-3-O-galactoside, and delphinidin-3-O-galactose were prepared at a concentration of 1 mg / mL. 10 μL of each solution was added dropwise to a 0.5 cm x 0.5 cm square cellulose acetate membrane. After drying at room temperature, the single-component attractant cores were obtained. These single-component attractant cores were sealed in aluminum foil and stored at -20°C.

[0023] According to the composition in Table 1, each component was dissolved in ultrapure water and dimethyl sulfoxide in a 1:1 v / v solvent to prepare an aqueous solution of the multi-component synergist. The total concentration of solute in the solution was 1 mg / mL. 10 μL of each solution was added dropwise onto a 0.5 cm x 0.5 cm square cellulose acetate membrane. After drying at room temperature, the attractant core was obtained. The prepared multi-component attractant core was sealed in aluminum foil and stored at -20°C.

[0024] A solvent was prepared by mixing ultrapure water and dimethyl sulfoxide in a 1:1 ratio. 10 μL of the solution was added dropwise onto a 0.5 cm x 0.5 cm square cellulose acetate membrane. After drying at room temperature, the control motif was obtained. The prepared control motif was sealed in aluminum foil and stored at -20 °C.

[0025] Prepare a 24-well plate. Place a 0.5cm x 0.5cm x 0.5cm piece of feed (formula shown in Table 2) in the center of each well. Place an attractant lure, a single-component lure, or a control lure on top. Select one 5th instar codling moth larva and place it at the edge of the well. Use this method to set 15 larvae for each type of lure. Cover the plate and time for 3 hours. Record the weight of the feed before and after the experiment. The difference in feed weight between the two measurements represents the amount of feed consumed by a single larva (mg). The experimental results are shown in Table 3.

[0026] In Table 3, the experimental data show that groups S-1 to S-4 represent the effects of lures prepared using a single compound; group CK represents the results of the solvent control lure; and group M represents the effects of a self-prepared multi-component attractant lure for codling moth larvae. The table shows that after using the multi-component attractant lure containing the codling moth larvae of this embodiment, the average feed intake of the codling moth larvae reached 20.13 mg. The average feed intake of larvae treated with the single-component attractant lure was 10.73–13.47 mg, and the average feed intake of larvae in the solvent control group was 12.80 mg. The multi-component attractant in this embodiment increased larval feeding by 57.26% compared to the solvent control. Statistical analysis was then performed... Figure 1 As can be seen, after treatment with the multi-component attractant formulation in this embodiment, the amount of feed consumed by the codling moth larvae was significantly higher than that of the solvent control and the single-component attractant (one-way ANOVA, F...). 5,84 =5.63, P=0.0002).

[0027] Table 1 Composition of multi-component appetite stimulants

[0028] Element molecular weight Molecular formula Standard concentration, % weight Isoquercetin 464.4 <![CDATA[C 21 H 20 O 12 ]]> 100 0.056 Phlorizin 436.4 <![CDATA[C 21 H 24 O 10 ]]> 100 0.84 cyanidin-3-O-galactoside 449.4 <![CDATA[C 21 H 21 ClO 11 ]]> 100 0.016 Larkspur-3-O-galactose 465.4 <![CDATA[C 21 H 21 O 12 ]]> 100 0.084

[0029] Table 2. Feed formulations used in the test.

[0030]

[0031] Table 3 Experimental Data

[0032]

[0033]

[0034] The above are merely preferred embodiments of the present invention. It should be noted that the above preferred embodiments should not be considered as limitations on the present invention, and the scope of protection of the present invention should be determined by the scope defined in the claims. For those skilled in the art, several improvements and modifications can be made without departing from the spirit and scope of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A bait composition for codling moth larvae, characterized in that, It includes the following components in parts by weight: isoquercitrin 0.02-0.07 parts, phlorizin 0.6-0.9 parts, cyanidin-3-O-galactoside 0.01-0.02 parts and delphinidin-3-O-galactose 0.05-0.12 parts.

2. The palatability-enhancing composition according to claim 1, characterized in that, The palatability enhancer composition comprises the following components in parts by weight: 0.056 parts isoquercitrin, 0.84 parts phlorizin, 0.016 parts cyanidin-3-O-galactoside, and 0.084 parts delphinidin-3-O-galactose.

3. The use of the attractant composition for codling moth larvae as described in claim 1 or 2 in attracting and / or controlling codling moths.

4. The application according to claim 3, characterized in that, The application involves applying the attractant composition of the codling moth larvae to a blank lure to attract the codling moth larvae; or applying the attractant composition of the codling moth larvae to a blank lure and then using the lure in combination with a chemical pesticide / biological pesticide.

5. A method for controlling the larvae of the codling moth, characterized in that, The method involves applying the attractant composition of the codling moth larvae as described in claim 1 or 2 to the habitat of the codling moth to attract the codling moth.

6. The method according to claim 5, characterized in that, The dosage of the attractant composition for codling moth larvae is 1–10 mg / m².

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