Application of chlorogenic acid in preparation of feed for inhibiting activity of algal toxin

Abstract

The invention discloses application of chlorogenic acid in preparation of feed for inhibiting activity of algal toxin, and belongs to the technical field of aquatic feed. According to the application of the chlorogenic acid in preparation of the feed for inhibiting the activity of the algal toxin, the chlorogenic acid in the feed can inhibit the activity of the algal toxin, the influence on the health of aquatic organisms after the aquatic organisms intake the algal toxin is effectively avoided, the incidence rate of diseases is reduced, and the survival rate of the organisms is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of aquatic feed, and in particular to application of chlorogenic acid in preparing feed for inhibiting the activity of algae toxins. Background Art

[0002] During the shrimp farming process, there is a critical growth stage - the liver transition period. When the shrimp fry grows to 4 to 6 cm, a layer of white capsule will appear around its liver. As the shrimp fry continues to grow, the white capsule gradually disappears and the capsule of its liver turns brown. This process is the liver transition period. During the liver transition period, shrimp are more sensitive to nutrition and environment and generally require higher water temperatures. At this time, algae in the water body multiply in large numbers, and the algae toxins produced will harm the health of the shrimp, causing the shrimp liver to shrink and the intestines to be empty, and finally causing a large number of shrimp deaths.

[0003] When using traditional feed to feed shrimp in the liver transition period, the shrimp are often easily affected by algal toxins, leading to liver diseases and affecting the growth, development and survival rate of the shrimp. Summary of the invention

[0004] In view of this, the object of the present invention is to provide an application of chlorogenic acid in the preparation of a feed for inhibiting the activity of algae toxins, so as to overcome the problem in the prior art that shrimps are easily harmed by algae toxins during the liver transition period and become sick or die.

[0005] In a first aspect, the present invention provides use of chlorogenic acid in preparing a feed for inhibiting the activity of algae toxins.

[0006] Compared with the prior art, the present invention applies chlorogenic acid to prepare feed. The chlorogenic acid in the feed can inhibit the activity of algae toxins, effectively avoid the impact on the health of aquatic organisms after ingesting algae toxins, reduce the incidence of diseases, and improve the survival rate of organisms.

[0007] Furthermore, the effective amount of chlorogenic acid for inhibiting the activity of algae toxins is 0.1 g to 0.4 g per kilogram of feed mass.

[0008] The above technical solution further limits the content of chlorogenic acid in the feed. The inventors found that when 0.1g to 0.4g of chlorogenic acid is contained in each kilogram of feed, the inhibitory effect of chlorogenic acid on the activity of algae toxins is optimal, which can further improve the survival rate of organisms.

[0009] Furthermore, chlorogenic acid is used in the form of a composition, which comprises chlorogenic acid, Lactobacillus plantarum, ethoxyquin and protein peptide in an effective amount for inhibiting the activity of algae toxins.

[0010] The above technical solution further limits the application of chlorogenic acid in the form of a composition. When the water body is contaminated by algal toxins, chlorogenic acid is combined with Lactobacillus plantarum, ethoxyquinoline and protein peptides. The four components cooperate with each other and work synergistically to further improve the health level and survival rate of aquatic organisms. Among them, chlorogenic acid can not only inhibit the activity of algal toxins, but also cooperate with Lactobacillus plantarum to improve the intestinal microecological environment of aquatic organisms, thereby promoting the absorption and utilization of nutrients; in addition, ethoxyquinoline and protein peptides in the composition can also effectively enhance the immunity of aquatic organisms, resist oxidative stress, and thus reduce the incidence of diseases.

[0011] Furthermore, based on the mass of each kilogram of feed, in the composition, the effective amount of chlorogenic acid for inhibiting the activity of algae toxins is 0.1g to 0.4g, Lactobacillus plantarum is 2.5 to 3g, ethoxyquin is 1.5 to 2g and protein peptide is 10 to 20g.

[0012] The above technical scheme further limits the content ratio of the four components of chlorogenic acid, Lactobacillus plantarum, ethoxyquin and protein peptide in the composition. Under the above content ratio, the synergistic effect of the four components is better, which can further reduce the damage caused by algal toxins to aquatic organisms.

[0013] In a second aspect, the present invention provides a feed for inhibiting the activity of algae toxins, comprising a basic feed and chlorogenic acid for inhibiting the activity of algae toxins.

[0014] Compared with the prior art, the beneficial effects of the feed for inhibiting the activity of algae toxins of the present invention are the same as the beneficial effects of the use of chlorogenic acid in the preparation of feed for inhibiting the activity of algae toxins, and will not be repeated here.

[0015] Furthermore, chlorogenic acid is used in the form of a composition comprising chlorogenic acid, Lactobacillus plantarum, ethoxyquin and protein peptide.

[0016] Furthermore, the basic feed includes the following components: protein substances, wheat flour, cellulose, oils, phospholipids, adhesives, choline chloride, complex vitamins and complex minerals.

[0017] The above technical solution further defines the specific components of the basic feed. The above components work synergistically to provide rich nutrients for shrimps, meet the shrimps' demand for nutrients during the liver transition period, and promote the growth and development of shrimps during the liver transition period.

[0018] Furthermore, the feed includes the following components, measured by mass per kilogram of feed: 450-550g of protein substances, 180-220g of wheat flour, 150-180g of cellulose, 45-55g of oils, 18-22g of phospholipids, 18-22g of adhesives, 8-12g of choline chloride, 8-12g of complex vitamins, 8-12g of complex minerals, 0.1-0.4g of chlorogenic acid, 2.5-3g of Lactobacillus plantarum, 1.5-2g of ethoxyquin and 10-20g of protein peptides.

[0019] The above technical solution further limits the content ratio of each component in the feed. Within the above content ratio range, it is more suitable for the nutritional needs of shrimp in the liver transition period and promotes the development of shrimp liver. In addition, chlorogenic acid, Lactobacillus plantarum, ethoxyquin and protein peptides within the appropriate content ratio range can further enhance the immunity of shrimp, resist oxidative stress, improve the intestinal microecological environment of shrimp, and inhibit the activity of algae toxins, thereby ensuring the health level of shrimp.

[0020] Furthermore, the binder is α-starch.

[0021] Furthermore, the above feed is used for feeding shrimps during the shrimp liver transition period, and the daily feeding amount is 6-8% of the shrimp body weight.

[0022] The above technical solution further limits the above breeding to be used for breeding shrimps in the liver transition period. Under the above feeding amount, the death of shrimps can be reduced and the survival rate of shrimps in the liver transition period can be improved. DETAILED DESCRIPTION

[0023] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0024] In a first aspect, an embodiment of the present invention provides the use of chlorogenic acid in preparing a feed for inhibiting the activity of algae toxins.

[0025] The above technical solution uses chlorogenic acid to prepare feed. The chlorogenic acid contained in the feed can inhibit the activity of algae toxins, reduce the impact of algae toxins on the health of aquatic organisms, and improve the survival rate of aquatic organisms in water bodies infected with algae toxins.

[0026] Further, based on the above embodiment, the embodiment of the present invention defines that the effective amount of chlorogenic acid for inhibiting the activity of algal toxins is 0.1g to 0.4g per kilogram of feed mass. For example, the chlorogenic acid may be 0.1g, 0.12g, 0.15g, 0.2g, 0.3g or 0.4g, preferably 0.1 to 0.15g. Chlorogenic acid can effectively inhibit the activity of algal toxins within the above effective content range. In particular, when the chlorogenic acid is 0.1 to 0.15g, it has a particularly excellent protective effect on the health of shrimp in the liver transition period, and can effectively improve the survival rate of shrimp in the liver transition period.

[0027] Further, based on the above embodiment, the embodiment of the present invention defines that chlorogenic acid is used in the form of a composition, and the composition comprises the chlorogenic acid, Lactobacillus plantarum, ethoxyquinoline and protein peptide in an effective amount to inhibit the activity of algae toxins. In the above composition, the effective amount of chlorogenic acid to inhibit the activity of algae toxins is 0.1-0.4g, Lactobacillus plantarum is 2.5-3g, ethoxyquinoline is 1.5-2g and protein peptide is 10-20g, based on the mass of each kilogram of feed.

[0028] For example, the chlorogenic acid in the composition may be 0.1g, 0.2g, 0.3g or 0.4g, the plant lactobacillus may be 2.5g, 2.6g, 2.7g, 2.8g, 2.9g or 3g, the ethoxyquin may be 1.5g, 1.6g, 1.7g, 1.8g, 1.9g or 2g, and the protein peptide may be 10g, 12g, 14g, 16g, 18g or 20g. Among them, the protein peptide is a zein peptide, and specifically, the protein peptide can be selected as Yihong peptide.

[0029] In the above technical scheme, chlorogenic acid is used in combination with Lactobacillus plantarum, ethoxyquin and protein peptides, which can resist oxidative stress and enhance the immunity of aquatic organisms on the basis of inhibiting the activity of algal toxins. At the same time, the synergistic effect of chlorogenic acid and Lactobacillus plantarum can also improve the intestinal microecological environment of aquatic organisms, promote the absorption and utilization of nutrients, and thus promote the growth and development of aquatic organisms.

[0030] In a second aspect, an embodiment of the present invention provides a feed for inhibiting the activity of algae toxins, comprising a basic feed and chlorogenic acid for inhibiting the activity of algae toxins.

[0031] Furthermore, chlorogenic acid is used in the form of a composition comprising chlorogenic acid, Lactobacillus plantarum, ethoxyquin and protein peptide.

[0032] On the basis of the above embodiments, the embodiments of the present invention further define that the basic feed includes the following components: protein substances, wheat flour, cellulose, oils, phospholipids, adhesives, choline chloride, complex vitamins and complex minerals.

[0033] Furthermore, it is defined that the feed includes the following components, measured by mass per kilogram of feed: 450-550g of protein substances, 180-220g of wheat flour, 140-180g of cellulose, 45-55g of oils, 18-22g of phospholipids, 18-22g of adhesives, 8-12g of choline chloride, 8-12g of complex vitamins, 8-12g of complex minerals, 0.1-0.4g of chlorogenic acid, 2.5-3g of Lactobacillus plantarum, 1.5-2g of ethoxyquin and 10-20g of protein peptides. For example, the protein material can be 450g, 470g, 500g, 520g or 550g, the wheat flour can be 180g, 190g, 200g, 210g or 220g, the oil can be 45g, 47g, 50g, 52g or 55g, the phospholipid can be 18g, 19g, 20g, 21g or 22g, the adhesive can be 18g, 19g, 20g, 21g or 22g, the choline chloride can be 8g, 9g, 10g, 11g or 12g, and the multivitamin can be 8 g, 9g, 10g, 11g or 12g, the complex minerals may be 8g, 9g, 10g, 11g or 12g, the chlorogenic acid may be 0.1g, 0.2g, 0.3g or 0.4g, the plant lactobacillus may be 2.5g, 2.6g, 2.7g, 2.8g, 2.9g or 3g, the ethoxyquin may be 1.5g, 1.6g, 1.7g, 1.8g, 1.9g or 2g, the protein peptide may be 10g, 12g, 14g, 16g, 18g or 20g, and the remainder is cellulose.

[0034] Among them, the protein material can be selected from fish meal and wheat gluten, and the mass ratio of fish meal to wheat gluten is (1.8-2.2): (2.8-3.2). The oil can be selected from fish oil and soybean oil, and the mass ratio of fish oil to soybean oil is (0.8-1.2): (0.8-1.2). The adhesive can be selected from α-starch.

[0035] In some embodiments, the composition of the complex vitamins in the feed, measured per kilogram of feed mass, may be: riboflavin: 45 mg; thiamine: 25 mg; vitamin K3: 10 mg; inositol: 800 mg; pyridoxine hydrochloride: 20 mg; vitamin B12: 0.1 mg; calcium pantothenate: 60 mg; biotin: 1.2 mg; vitamin A: 32 mg; vitamin D: 5 mg; niacin: 200 mg; folic acid: 20 mg; vitamin E: 120 mg.

[0036] In other embodiments, the composition of the complex minerals in the feed, measured by the mass of each kilogram of feed, can be: KI: 0.8 mg; sodium fluoride: 2.0 mg; Fe2(SO4)3: 80 mg; ZnSO4: 50 mg; CoCl2·6H2O: 50 mg; CuSO4·5H2O: 10.0 mg; magnesium sulfate: 200 mg; sodium chloride: 100 mg; Ca(H2PO4)2: 3000 mg.

[0037] On the basis of the above embodiment, the embodiment of the present invention further defines that the above feed is used for feeding shrimps during the shrimp liver transition period, and the daily feeding amount is 6-8% of the shrimp body weight.

[0038] Shrimp in the liver transition period are more sensitive to water temperature, water quality and nutritional status. The above-mentioned feed has a reasonable ratio of various components. When used for the feeding of shrimp during the liver transition period, it can not only provide sufficient nutritional support for shrimp in the liver transition period and promote the liver development of shrimp, but also the composition containing chlorogenic acid, Lactobacillus plantarum, ethoxyquin and protein peptide can reduce the stress response of shrimp, enhance the immunity of shrimp, improve the intestinal microecological environment of shrimp and inhibit the activity of algae toxins, thereby ensuring the health level of shrimp during the liver transition period and improving the survival rate of shrimp.

[0039] It should be understood that the raw materials used in the following examples are all commercially available raw materials unless otherwise specified.

[0040] Example 1

[0041] An embodiment of the present invention provides a feed for inhibiting the activity of algae toxins, comprising the following components: 200g fish meal, 300g wheat gluten, 200g wheat flour, 159.9g cellulose, 25g fish oil, 25g soybean oil, 20g phospholipids, 20g α-starch, 10g choline chloride, 10g complex vitamins, 10g complex minerals, 0.1g chlorogenic acid, 2.5g plant lactobacillus, 1.5g ethoxyquin and 16g Yihong peptide.

[0042] Example 2

[0043] An embodiment of the present invention provides a feed for inhibiting the activity of algae toxins, comprising the following components: 180g fish meal, 270g wheat gluten, 220g wheat flour, 177.85g cellulose, 27g fish oil, 28g soybean oil, 22g phospholipids, 22g α-starch, 8g choline chloride, 12g complex vitamins, 8g complex minerals, 0.15g chlorogenic acid, 3g plant lactobacillus, 2g ethoxyquin and 20g Yihong peptide.

[0044] Example 3

[0045] An embodiment of the present invention provides a feed for inhibiting the activity of algae toxins, comprising the following components: 220g fish meal, 330g wheat gluten, 180g wheat flour, 142.3g cellulose, 22g fish oil, 23g soybean oil, 18g phospholipids, 18g α-starch, 12g choline chloride, 8g complex vitamins, 12g complex minerals, 0.2g chlorogenic acid, 2.7g plant lactobacillus, 1.8g ethoxyquin and 10g Yihong peptide.

[0046] Example 4

[0047] An embodiment of the present invention provides a feed for inhibiting the activity of algae toxins, comprising the following components: 205g fish meal, 305g wheat gluten, 210g wheat flour, 146.1g cellulose, 24g fish oil, 24g soybean oil, 19g phospholipids, 21g α-starch, 11g choline chloride, 9g complex vitamins, 9g complex minerals, 0.4g chlorogenic acid, 2.8g Lactobacillus plantarum, 1.7g ethoxyquin and 12g Yihong peptide.

[0048] Comparative Example 1

[0049] The only difference from the feed for inhibiting algal toxin activity provided in Example 1 is that an equal amount of chlorogenic acid is replaced by Lactobacillus plantarum.

[0050] Comparative Example 2

[0051] The only difference from the feed for inhibiting algal toxin activity provided in Example 1 is that an equal amount of Lactobacillus plantarum is replaced by chlorogenic acid.

[0052] Comparative Example 3

[0053] The only difference from the feed for inhibiting algae toxin activity provided in Example 1 is that an equal amount of Yihong peptide is replaced by chicken bone peptide.

[0054] Comparative Example 4

[0055] The only difference from the feed for inhibiting algal toxin activity provided in Example 1 is that the added amount of chlorogenic acid is set to 0.6 g.

[0056] Test Case

[0057] 3600 Litopenaeus vannamei fry with strong physique, active feeding, no disease or injury were selected and divided into 8 groups, each with 450 shrimps, and fed with the feeds provided in Examples 1 to 4 and Comparative Examples 1 to 4, respectively.

[0058] The rearing of Penaeus vannamei is divided into two stages. The first stage is the breeding stage, in which the shrimp are raised in eight 12m 3 The above 8 groups of Penaeus vannamei fry were reared in cement pools. Before rearing, each cement pool was disinfected with potassium permanganate and cleaned. 6m 3The daily feed intake is 7% of the shrimp fry body weight. After 20 days of feeding, the shrimp enters the liver transition stage.

[0059] The second stage is the microcystin (MC-LR) attack stage. The above 8 groups of liver-transition Litopenaeus vannamei were injected with microcystin, and each shrimp was injected with 20ug of microcystin at a concentration of 500ug / kg. After the injection, they were transferred to 8 230L plastic storage boxes for feeding. Before feeding, each plastic storage box was disinfected with potassium permanganate and cleaned, and 200L of filtered seawater was added to each plastic storage box. The daily feeding amount of feed was 7% of the shrimp body weight. After 10 days of feeding, the growth performance and survival rate of the above 8 groups of shrimp were tested. The results are shown in Table 1.

[0060] Table 1

[0061]

[0062] From the above results, it can be seen that compared with Examples 1 to 4, after replacing the chlorogenic acid with Lactobacillus plantarum in an equal amount in Comparative Example 1 and replacing the Lactobacillus plantarum in an equal amount in Comparative Example 2 with chlorogenic acid, the weight, specific growth rate and survival rate of Penaeus vannamei after the liver transition period are reduced, indicating that the chlorogenic acid and Lactobacillus plantarum in the feed of the present invention have a synergistic effect, which can promote the absorption of nutrients and improve the health of shrimp, thereby improving the survival rate of shrimp. In Comparative Example 3, after replacing the Yihong peptide with chicken bone peptide in an equal amount, the weight, specific growth rate and survival rate of Penaeus vannamei after the liver transition period are reduced, indicating that adding corn protein peptide to the feed can further improve the immunity of shrimp, thereby reducing the morbidity of shrimp and improving the survival rate. In Comparative Example 4, after increasing the amount of chlorogenic acid added to 0.6g, the weight, specific growth rate and survival rate of Penaeus vannamei after the liver transition period are reduced, indicating that adding a specific amount of chlorogenic acid to the feed can further improve the survival rate of shrimp during the liver transition period.

[0063] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention is described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. Application of chlorogenic acid in the preparation of feed for inhibiting the activity of algae toxins.

2. The use according to claim 1, characterized in that: The effective amount of chlorogenic acid for inhibiting algal toxin activity is 0.1 g to 0.4 g per kilogram of the feed mass.

3. The use according to claim 1 or 2, characterized in that: The chlorogenic acid is used in the form of a composition, which comprises the chlorogenic acid, Lactobacillus plantarum, ethoxyquinoline and protein peptide in an effective amount for inhibiting the activity of algae toxins.

4. The use according to claim 3, characterized in that: Calculated per kilogram of the mass of the feed, in the composition, the effective amount of chlorogenic acid to inhibit algal toxin activity is 0.1g-0.4g, the plant lactobacillus is 2.5-3g, the ethoxyquin is 1.5-2g and the protein peptide is 10-20g.

5. A feed for inhibiting the activity of algae toxins, characterized in that: Includes basic feed and chlorogenic acid to inhibit the activity of algae toxins.

6. The feed according to claim 5, characterized in that The chlorogenic acid is applied in the form of a composition, which comprises chlorogenic acid, Lactobacillus plantarum, ethoxyquin and protein peptide.

7. The feed according to claim 5 or 6, characterized in that The basic feed comprises the following components: protein substances, wheat flour, cellulose, oils, phospholipids, adhesives, choline chloride, complex vitamins and complex minerals.

8. The feed according to claim 7, characterized in that The feed includes the following components, measured by weight per kilogram of the feed: 450-550 g of protein substances, 180-220 g of wheat flour, 150-180 g of cellulose, 45-55 g of oils, 18-22 g of phospholipids, 18-22 g of adhesives, 8-12 g of choline chloride, 8-12 g of complex vitamins, 8-12 g of complex minerals, 0.1-0.4 g of chlorogenic acid, 2.5-3 g of plant lactobacillus, 1.5-2 g of ethoxyquin and 10-20 g of protein peptides.

9. The feed according to claim 7, characterized in that The binder is α-starch.

10. The feed according to any one of claims 5 to 9, characterized in that: The feed is used for feeding shrimps during the shrimp liver transition period, and the daily feeding amount is 6-8% of the shrimp body weight.

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