Whitening composition and preparation method thereof
By using white tomato extract, grape seed extract and passion fruit extract, the problem of poor effect of existing whitening raw materials is solved, effectively inhibiting melanin production and transfer, and significantly improving the whitening effect.
Patent Information
- Application Number
- CN202510273089.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-10
- Publication Date
- 2025-04-25
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing whitening raw materials have poor effects and single targets and channels, resulting in individual differences and body state factors affecting the effect.
Using a whitening composition, including white tomato extract, grape seed extract and passion fruit extract, the mixture is premixed by a premix device to form a solid beverage or premix.
This whitening composition can inhibit the activity of tyrosinase, prevent melanin production, destroy the structure of melanosomes, cause melanocyte poisoning and apoptosis, and jointly prevent melanin transfer, significantly improve the whitening effect.
Smart Images
Figure CN119867309A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of whitening and beauty, and in particular to a whitening composition and a preparation method thereof. Background Art
[0002] As people's living standards improve, the pace of life speeds up, and the pressure of life and work increases, people are accustomed to staying up late and working overtime, which leads to more accumulation of melanin in the skin, making the skin appear dull, sallow, blackened, and spotted, resulting in poor mental outlook.
[0003] In the prior art, there are many raw materials used for whitening, but the effect of a single whitening raw material is poor, and the target and channel are single, which leads to the raw material being affected by various factors such as individual differences of the target group and body conditions, thus affecting the effect of the raw material. Summary of the invention
[0004] The purpose of the present invention is to provide a whitening composition and a preparation method, aiming to solve the technical problem in the prior art that a single whitening raw material has a poor effect and a single target and channel, which leads to the raw material being affected by various factors such as individual differences of the target group and body state, thus affecting the effect of the raw material.
[0005] To achieve the above purpose, the present invention adopts a whitening composition, which includes the following raw materials in parts by weight: 200g white tomato extract, 100g grape seed extract and 700g passion fruit extract.
[0006] Among them, the content of lycopene in white tomato extract is 18%-22%.
[0007] Among them, in grape seed extract, the content of proanthocyanidins is 70% and the content of cucurbitacinol is 10%.
[0008] Among them, the content of piceatannol in passion fruit extract is 5%.
[0009] The present invention also provides a method for preparing a whitening composition, which is used to prepare the whitening composition as described above. The steps include: First, take white tomato extract, grape seed extract and passion fruit extract in proportion and set aside; Select premixing equipment and clean it for use; The obtained raw materials are added into a premixing device for premixing to obtain a mixture, and then a premix or a solid beverage is prepared.
[0010] Among them, the lycopene content of the white tomato extract is 20%.
[0011] Among them, the lycopene content of the white tomato extract is 18%.
[0012] An experimental method is used to test the whitening composition described above, including a tyrosinase activity inhibition experiment, a melanin protein content reduction experiment and a melanosome reduction experiment.
[0013] The present invention discloses a whitening composition and a preparation method thereof. First, 200g of white tomato extract, 100g of grape seed extract and 700g of passion fruit extract are taken in proportion for later use; a premixing device is selected and cleaned for later use; the taken raw materials are added into the premixing device for premixing to obtain a mixture, and then a premix or a solid beverage is prepared. Experiments have shown that the whitening composition of the present invention can inhibit the activity of tyrosinase, and can reduce various intermediates produced in the process of melanin generation, and prevent the generation of melanin. The whitening composition of the present invention can destroy the structure of melanosomes, and cause melanocyte poisoning and apoptosis, and synergistically prevent the transfer of melanin. In this way, the technical problem that a single whitening raw material has a poor effect, and the target and channel are single in the prior art is solved, and the raw material is affected by various factors such as individual differences of the target group and the body state, thereby affecting the effect of the raw material. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.
[0015] Figure 1 It is a schematic diagram of the zebrafish animal test of the present invention. DETAILED DESCRIPTION
[0016] Embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the present invention, but should not be construed as limiting the present invention.
[0017] The invention provides a whitening composition, which comprises the following raw materials in parts by weight: 200g of white tomato extract, 100g of grape seed extract and 700g of passion fruit extract.
[0018] Among them, the content of lycopene in white tomato extract is 18%-22%.
[0019] Among them, in grape seed extract, the content of proanthocyanidins is 70% and the content of cucurbitacinol is 10%.
[0020] Among them, the content of piceatannol in passion fruit extract is 5%.
[0021] The present invention also provides a method for preparing a whitening composition, which is used to prepare the whitening composition as described above. The steps include: First, take white tomato extract, grape seed extract and passion fruit extract in proportion and set aside; Select premixing equipment and clean it for use; The obtained raw materials are added into a premixing device for premixing to obtain a mixture, and then a premix or a solid beverage is prepared. Example
[0022] In this embodiment, the preparation method of the whitening composition is as follows: S1. First, take 200g of white tomato extract, 100g of grape seed extract and 700g of passion fruit extract in proportion for later use; According to this specific embodiment, the lycopene content of the white tomato extract is 20%.
[0023] S2. Select the premixing equipment and clean it for use; S3, adding the obtained raw materials into a premixing device to premix to obtain a mixture, and then preparing a premix or a solid beverage. Example
[0024] In this embodiment, the preparation method of the whitening composition is as follows: S1. First, take 200g of white tomato extract, 100g of grape seed extract and 700g of passion fruit extract in proportion for later use; According to this specific embodiment, the lycopene content of the white tomato extract is 18%.
[0025] S2. Select the premixing equipment and clean it for use; S3, adding the obtained raw materials into a premixing device to premix to obtain a mixture, and then preparing a premix or a solid beverage.
[0026] See also Figure 1 , Figure 1 It is a schematic diagram of the zebrafish animal test of the present invention.
[0027] An experimental method is used to test the whitening composition described above, including a tyrosinase activity inhibition experiment, a melanin protein content reduction experiment and a melanosome reduction experiment.
[0028] Specifically, the tyrosinase activity inhibition experiment is as follows: The solid beverage was accurately weighed to prepare 0.50g / L, 1.00g / L, 1.50mg / L, and 2.00mg / L standard concentration liquids, and placed in 0-4℃ for later use. Then the tyrosinase activity was determined: The homogenate was centrifuged at 10 000×g for 5 min, and 100 μL of the supernatant was placed in a 96-well plate. At the same time, 1.00 mmol / L benzyl alcohol solution was added and incubated at 30 °C for 1 h. At 30 °C, tyrosinase was most active in 1.00 mmol / L benzyl alcohol solution and had the highest absorbance at a wavelength of 412 nm. The absorbance of each experimental group was detected using an enzyme marker (412 nm). The tyrosinase activity inhibition rate was calculated as follows: inhibition rate = A = (1−A drug treatment group −A blank control group / A solvent control group −A blank control group) * 100%. The temperature test showed that the activity of tyrosinase was inversely proportional to its absorbance. As the standard concentration of the "whitening composition" was added to the tyrosinase benzyl alcohol solution, its absorbance gradually decreased. Therefore, it was concluded that the "whitening composition" could inhibit the activity of tyrosinase, and the inhibitory activity became stronger as the amount added increased.
[0029] The experiment to reduce the content of melanin protein is as follows: The above-mentioned "whitening composition" solid beverage was accurately weighed and then mixed into 500 mg of feed according to the addition ratio of 50 mg, 100 mg, 150 mg, and 200 mg, and granulated.
[0030] Then, a zebrafish experiment was conducted, and melanocyte estrogen was injected into the yolk sac to establish a zebrafish melanin model of 30 fish. The fish were fed once a day. After four weeks, 10 zebrafish were randomly selected, pretreated, and oscillated to extract the melanin protein in the zebrafish for later use.
[0031] Then the melanin content was determined: the homogenate was centrifuged at 10 000×g for 5 min, and the precipitate was used for melanin content determination. 150 μL NaOH solution (1 mol / L) was added to the EP tube, heated in a 100 ℃ water bath for 10 min, and vibrated to fully dissolve the melanin in the EP tube. 100 μL of the solution was placed in a 96-well plate, and the absorbance was measured at 405 nm using an ELISA reader. The formula for calculating the inhibition rate of melanin synthesis is: Inhibition rate = A = (1−A drug treatment group −A blank control group / A solvent control group −A blank control group) * 100% Statistical analysis: SPSS 19.0 software was used to statistically process the data, and the experimental data were expressed as x ± SEM data, and one-way analysis of variance was used. Pairwise comparison of each concentration group with the blank control group: *P < 0.05.
[0032] By testing the absorbance value of melanin protein at a wavelength of 405nm using a multifunctional microplate reader, the experiment proved that the addition amount of the "whitening composition" solid beverage is inversely correlated with the melanin protein content in zebrafish. The "whitening composition" can reduce the melanin protein content in zebrafish, and the reducing effect is obvious as the addition amount increases.
[0033] The black body reduction experiment is as follows: The above-mentioned "whitening composition" solid beverage was accurately weighed, and then mixed into 500 mg of feed according to the addition ratio of 50 mg, 100 mg, 150 mg, and 200 mg, and granulated.
[0034] Then, a zebrafish experiment was conducted, and melanocyte estrogen was injected into the yolk sac to establish a zebrafish melanin model of 30 fish. The fish were fed with feed once a day. After four weeks, 10 zebrafish were randomly selected and observed under an optical microscope after pretreatment. The data were analyzed and collected using Image J advanced image processing software to analyze the signal intensity of the melanosomes in the zebrafish head. The statistical analysis results of this indicator showed that the "whitening composition" can effectively reduce the melanosomes in the zebrafish body, thereby inhibiting, degrading and preventing the transfer of melanosomes.
[0035] Non-toxic experiments on whitening compositions: MTC experiment: Zebrafish were kept in fish farming water at 28°C (water quality: 200 mg instant sea salt was added to every 1 L of reverse osmosis water, conductivity was 450-550 μS / cm; pH was 6.5-8.5; hardness was 50-100 mg / L CaCO3); zebrafish aged 3 days post fertilization (3 dpf) were used for the determination of the maximum detection concentration (MTC) of sample X and its efficacy evaluation (phenotypic observation, melanin protein content detection and tyrosinase activity detection).
[0036] The zebrafish used in the experiment were bred and provided by our company's fish farming center. The experimental animal use license number is: SYXK (Zhejiang) 2022-0004. The breeding and management are in compliance with the requirements of the international AAALAC certification (certification number: 001458). Wild-type AB strain zebrafish were bred in natural pairs.
[0037] A whitening composition and preparation method of the present invention are used. First, 200g of white tomato extract, 100g of grape seed extract and 700g of passion fruit extract are taken in proportion for later use; a premixing device is selected and cleaned for later use; the taken raw materials are added into the premixing device for premixing to obtain a mixture, and then a premix or a solid beverage is prepared. Experiments have shown that the whitening composition of the present invention can inhibit the activity of tyrosinase, and can reduce various intermediates produced in the process of melanin production, and prevent the production of melanin. The whitening composition of the present invention can destroy the structure of melanosomes, cause melanocyte poisoning and apoptosis, and synergistically prevent the transfer of melanin. In this way, the technical problem that a single whitening raw material has a poor effect and a single target and channel in the prior art is solved, which leads to the raw material being affected by various factors such as individual differences of the target group and body state, thereby affecting the effect of the raw material.
[0038] What is disclosed above is only a preferred embodiment of the present invention, and it certainly cannot be used to limit the scope of rights of the present invention. Ordinary technicians in this field can understand that all or part of the processes of the above embodiment and equivalent changes made according to the claims of the present invention still fall within the scope of the invention.
Claims
1. A whitening composition, characterized in that: The invention comprises the following raw materials in parts by weight: 200 g of white tomato extract, 100 g of grape seed extract and 700 g of passion fruit extract.
2. The whitening composition according to claim 1, characterized in that The lycopene content in white tomato extract is 18%-22%.
3. The whitening composition according to claim 2, characterized in that In grape seed extract, the content of proanthocyanidins is 70% and the content of cucurbitacin is 10%.
4. The whitening composition according to claim 3, characterized in that In passion fruit extract, the content of piceatannol is 5%.
5. A method for preparing a whitening composition, for preparing the whitening composition according to claim 4, characterized in that: The steps include: First, take 200g of white tomato extract, 100g of grape seed extract and 700g of passion fruit extract in proportion and set aside; Select premixing equipment and clean it for use; The obtained raw materials are added into a premixing device for premixing to obtain a mixture, and then a premix or a solid beverage is prepared.
6. The method for preparing the whitening composition according to claim 5, characterized in that: The lycopene content of the white tomato extract is 20%.
7. The method for preparing the whitening composition according to claim 5, characterized in that: The lycopene content of the white tomato extract is 18%.
8. An experimental method for testing the whitening composition according to claim 4, characterized in that: Including tyrosinase activity inhibition experiment, melanin protein content reduction experiment and melanosome reduction experiment.