Preparation method and application of Terminalia chebula Retz. extract

Abstract

The present invention belongs to the technical field of daily-use cosmetics, and particularly relates to a preparation method and application of Terminalia chebula extract. The Terminalia chebula extract contains gallic acid and chebulinic acid, wherein the contents of gallic acid and chebulinic acid are ≥20 wt%, calculated based on the dry weight of the Terminalia chebula extract. In the present invention, the Terminalia chebula extract can simultaneously enrich two active substances, gallic acid and chebulinic acid, to the greatest extent through the type of composite macroporous resin. Its transfer rate is increased by 10.64% compared with a single resin, and the yield of the freeze-dried powder of the obtained Terminalia chebula extract is increased by 13.99% compared with a single resin. In addition, the prepared Terminalia chebula extract has the effects of inhibiting 5α-reductase, melanin secretion, Streptococcus mutans, and Porphyromonas gingivalis, proving that the Terminalia chebula extract has the effects of oil control, sun protection, whitening and brightening, and antibacterial, providing the possibility and basis for its application in the field of daily-use cosmetics.

Description

Technical Field

[0001] The invention belongs to the technical field of daily cosmetics, and particularly relates to a preparation method and application of a terminalia chebula extract. Background Art

[0002] Terminalia chebula is the dried mature fruit of Terminalia chebula Retz. or Terminalia chebula Retz, var. tomentella Kurt., a plant of the Combretaceae family, and Xiqingguo is the dried young fruit of Terminalia chebula, a plant of the Combretaceae family. The chemical components of the two medicinal materials are mainly tannins, triterpenes, phenolic acids and fatty acids, among which the tannin content is as high as 23% to 37%. "The King of Tibetan Medicine" - Terminalia chebula. In the Yongkang Town of Yongde County, there grows a tree called Terminalia chebula, which is known as the "King of Tibetan Medicine". In the classic work of Tibetan medicine "Jingzhu Materia Medica", Terminalia chebula is called the "King of Tibetan Medicine" and is the most commonly used Tibetan medicine.

[0003] Patent CN110772550A prepares ellagic acid by pulp powder, ethanol reflux, and single macroporous resin NKA-9 or HPD600. Since the polyphenolic components of gallic acid and chebula tannic acid are more polar than ellagic acid, the preparation method of this patent has a low adsorption rate of active ingredients of gallic acid and chebula tannic acid, and the obtained extract is mainly ellagic acid. Patent CN111658683A provides an application of a chebula water extract in the preparation of a drug for treating and / or preventing Malassezia infection-related skin diseases, which is mainly used in Malassezia infection-related skin diseases including pityriasis versicolor, Malassezia folliculitis and seborrheic dermatitis. The chemical components contained in chebula are rich and diverse, mainly including tannins, phenolic acids, triterpenes, flavonoids, volatile oils and other components. Modern pharmacological studies have shown that its extract has multiple pharmacological activities such as antioxidant, anti-diabetic, antibacterial, antiviral, anti-inflammatory, and analgesic. However, in the field of daily chemical products, there are few studies on the application of Terminalia chebula extract in oil control, sun protection, whitening and oral care.

[0004] Therefore, the art is in urgent need of developing applications of terminalia chebula extract in oil control, sun protection, whitening and oral care. Summary of the invention

[0005] The present invention aims to provide application of a terminalia chebula extract in oil control, sun protection, whitening and oral care, and specifically relates to a preparation method and application of the terminalia chebula extract.

[0006] In a first aspect of the present invention, a terminalia chebula extract is provided, wherein the terminalia chebula extract comprises gallic acid and terminalia tannic acid, wherein the total content of the gallic acid and terminalia tannic acid is greater than 20 wt %, based on the dry weight of the terminalia chebula extract.

[0007] In another preferred embodiment, the total content of gallic acid and chebulinic acid is ≥ 25 wt%, based on the dry weight of the Terminalia chebula extract.

[0008] In another preferred embodiment, the total content of gallic acid and chebulinic acid is 20 wt% - 60 wt%, preferably 20 wt% - 50 wt%, more preferably 20 wt% - 40 wt%, based on the dry weight of the Terminalia chebula extract.

[0009] In another preferred embodiment, the content of gallic acid is 0.5 wt% - 3 wt%, preferably 1 wt% - 2.5 wt%, more preferably 1.5 wt% - 2 wt%, based on the dry weight of the Terminalia chebula extract.

[0010] In another preferred embodiment, the content of chebulinic acid is 15 wt% - 40 wt%, preferably 20 wt% - 35 wt%, more preferably 25 wt% - 30 wt%, based on the dry weight of the Terminalia chebula extract.

[0011] In another preferred embodiment, the Terminalia chebula extract further comprises chebulagic acid and / or ellagic acid, wherein the content of chebulagic acid is 10 - 20 wt%, more preferably 15 - 20 wt%.

[0012] In another preferred embodiment, the Terminalia chebula extract further comprises chebulagic acid and / or ellagic acid, wherein the content of ellagic acid is 1 - 10 wt%, more preferably 1 - 5 wt%.

[0013] In a second aspect of the present invention, there is provided a method for preparing the Terminalia chebula extract as described in the first aspect of the present invention, the method comprising the steps of:

[0014] (1) Extraction: Extract Terminalia chebula fruits with ethanol, collect the filtrate and concentrate to obtain a crude extract of Terminalia chebula extract;

[0015] (2) Purification: Take the crude extract obtained in step (1), elute with a combined macroporous resin of S-8 and NKA-9, and the elution includes: first elute with water, collect the water eluate, and then elute with ethanol, collect the ethanol eluate;

[0016] (3) Take the water eluate obtained in step (2), use ethanol as the eluent, and continue to elute with a combined macroporous resin of S-8 and NKA-9 to obtain an ethanol eluate;

[0017] (4) Combine the ethanol eluates obtained in steps (2) and (3), and lyophilize to obtain the Terminalia chebula extract.

[0018] In another preferred embodiment, the ethanol is an aqueous ethanol solution with a concentration of 60 v / v % - 70 v / v %.

[0019] In another preferred embodiment, the macroporous resin is selected from the group consisting of: S-8, NKA-9, or a combination thereof. In another preferred embodiment, the method comprises the following steps:

[0020] (1) Provide raw Terminalia chebula fruits, add ethanol with a concentration of 50 - 80 v / v % for extraction, collect the extract, concentrate it to obtain a crude extract paste of Terminalia chebula extract; dilute the crude extract paste of Terminalia chebula extract with water to obtain a sample loading solution;

[0021] (2) Load the sample loading solution onto the macroporous resin for resin adsorption, and perform elution. Collect the elution effluent containing gallic acid and chebulinic acid to obtain a Terminalia chebula extract rich in gallic acid and chebulinic acid, wherein the macroporous resin is a composite macroporous resin of S-8 and NKA-9, and the elution includes: first elute with water, collect the water eluate, then elute with ethanol, collect the ethanol eluate, so as to obtain an eluate rich in gallic acid and chebulinic acid; and

[0022] (3) Optionally, concentrate the eluate to obtain a Terminalia chebula extract rich in gallic acid and chebulinic acid.

[0023] In another preferred embodiment, in the sample loading solution, the content of the crude extract paste of Terminalia chebula extract is 40 - 60 v / v %.

[0024] In another preferred embodiment, in the sample loading solution, the content of the crude extract paste of Terminalia chebula extract is 45 - 55 v / v %.

[0025] In another preferred embodiment, the volume ratio of the crude extract paste of Terminalia chebula extract to the composite macroporous resin is 1:(2 - 6).

[0026] In another preferred embodiment, the volume ratio of the crude extract paste of Terminalia chebula extract to the composite macroporous resin is 1:(3 - 5).

[0027] In another preferred embodiment, the method for preparing the Terminalia chebula extract as described in the first aspect of the present invention comprises the steps:

[0028] (1) Extraction: Provide raw Terminalia chebula fruits, add ethanol with a concentration of 60 v / v % - 70 v / v % for reflux extraction, collect the filtrate, concentrate it to obtain a crude extract paste of Terminalia chebula extract;

[0029] (2) Purification: Load the crude extract paste obtained in step (1) onto a combined macroporous resin of S-8 and NKA-9, and perform an elution procedure: first elute with water, collect the water eluate, then elute with ethanol with a concentration of 60 v / v % - 70 v / v %, collect the ethanol eluate;

[0030] (3) Take the water eluate obtained in step (2), repeat the elution procedure in step (2) 2 - 4 times, and combine the ethanol eluates obtained each time;

[0031] (4) Take the ethanol eluate obtained in step (3) and freeze-dry it to obtain the Terminalia chebula extract.

[0032] In another preferred example, the ratio of S-8 to NKA-9 in the composite macroporous resin of S-8 and NKA-9 is 6:1 to 1:6 (w:w).

[0033] In another preferred example, the ratio of S-8 to NKA-9 in the composite macroporous resin of S-8 and NKA-9 is 1 to 3-5 (w:w).

[0034] In another preferred example, the ratio of S-8 to NKA-9 in the composite macroporous resin of S-8 and NKA-9 is (2-1) to (2-1) (w:w).

[0035] In another preferred example, the ratio of S-8 to NKA-9 in the composite macroporous resin of S-8 and NKA-9 is 3-5 to 1 (w:w).

[0036] In another preferred example, in the Terminalia chebula extract rich in gallic acid and chebulinic acid, the transfer rate F1 of gallic acid and chebulinic acid is ≥80 wt%, preferably, F1 ≥90 wt%.

[0037] In another preferred example, the transfer rate F1 is calculated according to the following formula Q1:

[0038] F1 = Fm + Fh Q1

[0039] In the formula,

[0040] Fm is the transfer rate of gallic acid in the Terminalia chebula extract rich in gallic acid and chebulinic acid;

[0041] Fh is the transfer rate of chebulinic acid in the Terminalia chebula extract rich in gallic acid and chebulinic acid.

[0042] In another preferred example, the transfer rate Fm is calculated according to the following formula Q2:

[0043] Fm = [Wm / W 浸膏 ×100% Q2

[0044] In the formula:

[0045] Wm is the mass of gallic acid in the Terminalia chebula extract rich in gallic acid and chebulinic acid;

[0046] W 浸膏 is the mass of the crude extract of the Terminalia chebula extract.

[0047] In another preferred example, the transfer rate Fh is calculated according to the following formula Q3:

[0048] Fh = [Wh / W 浸膏 × 100% Q3

[0049] Wherein:

[0050] Wh is the mass of chebulinic acid in the Terminalia chebula extract rich in gallic acid and chebulinic acid;

[0051] W 浸膏 is the mass of the crude extract of Terminalia chebula extract.

[0052] In another preferred example, the method further includes vacuum drying or freeze drying the eluate rich in gallic acid and chebulinic acid to obtain a powdery Terminalia chebula extract.

[0053] In another preferred example, in the Terminalia chebula extract rich in gallic acid and chebulinic acid, the yield of gallic acid and chebulinic acid and H1 ≥ 20 wt%, preferably, H1 ≥ 25 wt% (based on dry weight).

[0054] In another preferred example, the yield and H1 are calculated according to the following formula Q4:

[0055] H1 = [Wh / W 原料 × 100% Q4

[0056] Wherein:

[0057] Wh is the mass of the Terminalia chebula extract rich in gallic acid and chebulinic acid.

[0058] W 原料 is the mass of the Terminalia chebula raw material.

[0059] In another preferred example, step (2) in the method includes:

[0060] (S1) Loading the sample solution onto a composite macroporous resin for resin adsorption;

[0061] (S2) First eluting with water, collecting the water eluate A, and then eluting with ethanol with a concentration of 60 - 70 wt%, collecting the ethanol eluate B;

[0062] (S3) Taking the water eluate A obtained in step (S1) as the sample solution A1, and loading it onto a composite macroporous resin for resin adsorption;

[0063] (S4) Repeating steps (S2 - S3) 2 - 4 times, collecting the ethanol eluate obtained each time, or mixing the ethanol eluates obtained each time in any proportion, and concentrating to obtain a Terminalia chebula extract rich in gallic acid and chebulinic acid;

[0064] Among them, the macroporous resin is a composite macroporous resin of S - 8 and NKA - 9.

[0065] In another preferred example, in step (S2) of the method, the washing liquid A corresponding to 2 bed volumes (BV) of water washing is collected.

[0066] In another preferred example, in step (S2) of the method, the ethanol eluate B corresponding to 4 bed volumes (BV) of ethanol washing is collected.

[0067] In another preferred example, in step (S3) of the method, the volume ratio of the washing liquid A to the macroporous composite resin is 1:(3 - 5).

[0068] In another preferred example, step (2) of the method includes:

[0069] (S1) Loading the sample solution onto the macroporous composite resin for resin adsorption;

[0070] (S2) First eluting with water, first washing with 2 BV of water and collecting the washing liquid A, then eluting with 4 BV of 65% ethanol and collecting the ethanol eluate A1;

[0071] (S3) Purifying the washing liquid A with 4 times the feed amount of the macroporous composite resin; repeating (S2) to obtain the washing liquid B and the ethanol eluate B1;

[0072] (S4) Purifying the washing liquid B with 4 times the feed amount of the macroporous composite resin; repeating (S2) to obtain the washing liquid C and the ethanol eluate C1;

[0073] (S5) Collecting the ethanol eluates A1, B1, and C1, or mixing the ethanol eluates obtained each time in any proportion and concentrating to obtain the Terminalia chebula extract rich in gallic acid and chebulinic acid.

[0074] In another preferred example, the elution procedure in step (2) is repeated 3 times, and the elution procedure includes the steps:

[0075] (2 - 1) First eluting with water and collecting the washing liquid 1, then eluting with ethanol with a concentration of 60 v / v% - 70 v / v% and collecting the ethanol eluate 1;

[0076] (2 - 2) Taking the washing liquid 1 obtained in step (2 - 1), first eluting with water and collecting the washing liquid 2, then eluting with ethanol with a concentration of 60 v / v% - 70 v / v% and collecting the ethanol eluate 2;

[0077] (2 - 3) Taking the washing liquid 2 obtained in step (2 - 1), first eluting with water and then eluting with ethanol with a concentration of 60 v / v% - 70 v / v% and collecting the ethanol eluate 3, and combining the ethanol eluates obtained each time.

[0078] In another preferred example, the Terminalia chebula fruit in step (1) is the young fruit of Terminalia chebula.

[0079] In another preferred embodiment, the Terminalia chebula fruits described in step (1) are young Terminalia chebula fruits or mature Terminalia chebula fruits.

[0080] In another preferred embodiment, the Terminalia chebula fruits described in step (1) are young Terminalia chebula fruits, or mature Terminalia chebula fruits or Terminalia chebula pulp (pitted).

[0081] In another preferred embodiment, the extraction in step (1) is heat reflux extraction.

[0082] In another preferred embodiment, the time for reflux extraction in step (1) is 1.5 - 2.5 h.

[0083] In another preferred embodiment, in step (2), the resin dosage of the combined macroporous resin is S - 8:NKA - 9 = 10:1 to 10:1 (w:w).

[0084] In another preferred embodiment, in step (2), the resin dosage of the combined macroporous resin is S - 8:NKA - 9 = 6:1 to 1:6 (w:w).

[0085] In another preferred embodiment, in step (2), the mass ratio of the crude extract of the extract to the dosage of the combined macroporous resin is 20% - 150%.

[0086] In the third aspect of the present invention, there is provided the use of the Terminalia chebula extract as described in the first aspect of the present invention for preparing a daily product or a daily chemical product having the functions of oil control, sun protection, whitening or antibacterial.

[0087] In another preferred embodiment, the composition is used to inhibit 5α - reductase.

[0088] In another preferred embodiment, the composition further has the function of oil control.

[0089] In another preferred embodiment, the inhibition rate of the composition on 5α - reductase is ≥ 35%.

[0090] In another preferred embodiment, the composition is used for sun protection.

[0091] In another preferred embodiment, the sun protection index SPF value of the composition is ≥ 5.5.

[0092] In another preferred embodiment, the composition is used to reduce the melanin content.

[0093] In another preferred embodiment, the product further has one or several characteristics selected from the following group:

[0094] (1) Used to inhibit 5α - reductase;

[0095] (2) The sun protection index SPF value > 5.5;

[0096] (3) Reducing the melanin content:

[0097] (4)Suppress microorganisms selected from the group consisting of Streptococcus mutans and Porphyromonas gingivalis.

[0098] In the fourth aspect of the present invention, there is provided a composition comprising: (1) the Terminalia chebula extract as described in the first aspect of the present invention; and (2) additional ingredients that can be used in daily chemical products.

[0099] In another preferred embodiment, the composition is used for the uses selected from the group consisting of oil control, sunscreen, whitening, and antibacterial.

[0100] In another preferred embodiment, the composition is a sunscreen composition.

[0101] In another preferred embodiment, the composition is an antidandruff composition.

[0102] In another preferred embodiment, the composition is used to inhibit microorganisms selected from the group consisting of Streptococcus mutans, Porphyromonas gingivalis, or a combination thereof.

[0103] In another preferred embodiment, the composition is a cream or an emulsion.

[0104] In another preferred embodiment, the composition is selected from the group consisting of facial cream, skin care lotion, primer, emulsion, facial mask, toothpaste, shampoo, mouthwash, and sunscreen.

[0105] It should be understood that within the scope of the present invention, the above-mentioned technical features of the present invention and the technical features specifically described below (such as in the examples) can be combined with each other to form new or preferred technical solutions. Due to space limitations, they will not be elaborated one by one here. Description of the Drawings

[0106] Figure 1 Shows the HPLC chromatogram of the Terminalia chebula extract described in the examples of the present invention.

[0107] Figure 2 Shows the inhibition rate of the Terminalia chebula extract on 5α-reductase.

[0108] Figure 3 Shows the viability of the Terminalia chebula extract on mouse B16-F10 melanoma cells.

[0109] Figure 4 Shows the effect of the Terminalia chebula extract on the secretion of melanin by mouse B16-F10 cells. Detailed Description of the Invention

[0110] After extensive and in-depth research and a large number of experimental screenings, the present inventor unexpectedly discovered for the first time that when ethanol is used as the extraction solvent for reflux extraction of Terminalia chebula Retz., the solvent is safe and non-toxic, and through the type of composite macroporous resin, two active substances, gallic acid and chebulinic acid, can be enriched simultaneously to the greatest extent, and the contents of the active ingredients gallic acid and chebulinic acid in the obtained Terminalia chebula Retz. extract are ≥ 20 wt%. In the present invention, during the purification process, a mixed macroporous resin is used for purification. The transfer rates of the active substances gallic acid and chebulinic acid obtained are increased by 10.64% compared with a single resin, and the yield of the freeze-dried powder of the obtained Terminalia chebula Retz. extract is increased by 13.99% compared with a single resin. In addition, the prepared Terminalia chebula Retz. extract has the effects of inhibiting 5α-reductase, melanin secretion, Streptococcus mutans and Porphyromonas gingivalis, indicating that the said Terminalia chebula Retz. extract has the effects of controlling oil, sun protection, whitening and brightening, and antibacterial, providing the possibility and basis for its application in the field of daily-use cosmetics. On this basis, the present inventor completed the present invention.

[0111] Terminology Explanation

[0112] Terminalia chebula Retz. extract

[0113] In the present invention, the active ingredients in the said Terminalia chebula Retz. extract include gallic acid and chebulinic acid, and their structural formulas are shown as follows.

[0114]

[0115] In the present invention, through the type of composite macroporous resin, two active substances, gallic acid and chebulinic acid, can be enriched simultaneously to the greatest extent in the Terminalia chebula Retz. extract, and the contents of gallic acid and chebulinic acid in the obtained Terminalia chebula Retz. extract are ≥ 20 wt%, calculated based on the dry weight of the Terminalia chebula Retz. extract.

[0116] Preparation Method of Terminalia chebula Retz. Extract

[0117] Weigh the raw materials Terminalia chebula Retz. or Terminalia myriocarpa Hance var. tomentella Kurt., add 6 times (the mass of the feeding amount in kg / the volume of the solvent in L) of 65% ethanol, and reflux at 70 °C - 85 °C for 2 h; extract twice; filter; concentrate under reduced pressure to recover alcohol (55 °C - 80 °C, vacuum pressure 0.060 - 0.080 Mpa) to obtain a crude extract; purify the crude extract with a mixed macroporous resin of S-8:NKA-9 = 1:4 with 4 times the feeding amount. After mixing the crude extract with 1 volume of water, load the sample, first wash with 2 BV of water to obtain washing solution A, and then elute with 4 BV of 65% ethanol to obtain eluate A1; purify washing solution A with a mixed macroporous resin of S-8:NKA-9 = 1:4 with 4 times the feeding amount, first wash with 2 BV of water to obtain washing solution B, and then elute with 4 BV of 65% ethanol to obtain eluate B1; purify washing solution B with a mixed macroporous resin of S-8:NKA-9 = 1:4 with 4 times the feeding amount, first wash with 2 BV of water to obtain washing solution B, and then elute with 4 BV of 65% ethanol to obtain eluate C1. Obtain eluates A1, B1, and C1, which can be concentrated separately, or the extracts can be concentrated after mixing A1, B1, and C1 in any proportion to obtain an extract; obtain the Terminalia chebula Retz. extract powder by vacuum drying or freeze drying.

[0118] Use of Terminalia chebula Retz. extract

[0119] The Terminalia chebula Retz. extract described in the present invention enriches two active compounds, gallic acid and chebulinic acid, and has rich active effects, and its effects include but are not limited to:

[0120] a) Oil control: The Terminalia chebula Retz. extract described above can inhibit the activity of 5α-reductase, and the inhibition rate is ≥ 35%, so it has the effect of oil control;

[0121] b) Sunscreen: The sun protection index SPF value of the Terminalia chebula Retz. extract described above is 5.67, the measured value of the UVA PFA value is 2.01, and the PA value is +, so it has a certain sun protection effect;

[0122] c) Whitening: The Terminalia chebula Retz. extract described above can inhibit the secretion of melanin by mouse melanoma cells, which is equivalent to kojic acid, so it has the effect of whitening;

[0123] d) Broad-spectrum antibacterial: The Terminalia chebula Retz. extract described above has the effect of inhibiting Streptococcus mutans and Porphyromonas gingivalis, and the MIC concentration is lower than 0.5 wt%, so it has the antibacterial effect.

[0124] Therefore, the Terminalia chebula Retz. extract obtained by the method described in this application can be used to prepare daily or daily chemical products for oil control, sunscreen, whitening and brightening, and antibacterial. Preferably, in the daily or daily chemical products, the dosage of the Terminalia chebula Retz. extract in the daily or daily chemical products is 0.05 - 99 wt%.

[0125] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0126] 1. In the present invention, ethanol is used as the extraction solvent in the preparation process of the Terminalia chebula extract. The solvent is safe and non-toxic, and two active substances, gallic acid and chebulinic acid, can be simultaneously enriched to the greatest extent through the type of composite macroporous resin. Moreover, the contents of the active ingredients, gallic acid and chebulinic acid, in the Terminalia chebula extract are ≥ 20 wt%.

[0127] 2. In the present invention, the extraction method of the Terminalia chebula extract is the heating reflux extraction method. This method is simple, requires low equipment, and has low cost, making it suitable for industrial production.

[0128] 3. In the present invention, during the purification process, mixed macroporous resin is used for purification. The transfer rate of the active substances, gallic acid and chebulinic acid, obtained is increased by 10.64% compared with that of single resin; the yield of the freeze-dried powder of the Terminalia chebula extract obtained is increased by 13.99% compared with that of single resin.

[0129] 4. In the present invention, the Terminalia chebula extract has the effect of inhibiting 5α-reductase, and the inhibition rate of 5α-reductase is ≥ 35%, thus having the oil control effect.

[0130] 5. In the present invention, the Terminalia chebula extract has the effect of inhibiting Streptococcus mutans and Porphyromonas gingivalis.

[0131] 6. In the present invention, the Terminalia chebula extract has the effect of inhibiting melanin secretion, which is comparable to that of the common whitening ingredient kojic acid.

[0132] 7. In the present invention, the prepared Terminalia chebula extract has the effects of oil control, sun protection, whitening and brightening, and antibacterial, providing the possibility and basis for its application in the field of daily-use cosmetics.

[0133] The following further elaborates the present invention in conjunction with specific embodiments. It should be understood that these embodiments are only used to illustrate the present invention and not to limit the scope of the present invention. The experimental methods without specific conditions noted in the following embodiments are usually carried out under conventional conditions or according to the conditions recommended by the manufacturer. Unless otherwise specified, percentages and parts are by weight.

[0134] Example 1: Testing of Active Ingredients in Raw Materials

[0135] Precisely weigh 0.5 g each of the fine powder of young Terminalia chebula fruits and the fine powder of mature fruits that have passed through a 24-mesh sieve, add 50 mL of a methanol solution with a mass percentage concentration of 50%, ultrasonicate for 30 min, dilute the extract 10 times, and filter. Use high performance liquid chromatography to test the contents of gallic acid and chebulinic acid (calculated based on dry weight).

[0136] Table 1 Contents of Active Ingredients in Raw Materials

[0137]

[0138] As can be seen from the test data in Table 1, the contents of gallic acid and chebulinic acid in the young fruits of Terminalia chebula Retz. are higher than those in the mature fruits. Therefore, the young fruits of Terminalia chebula Retz. can be preferably used as raw materials for the extraction of active ingredients.

[0139] Example 2: Selection of extraction solvent

[0140] In this example, the young fruits of Terminalia chebula Retz. were used as raw materials, and Terminalia chebula Retz. was extracted with different solvents, and then the contents of gallic acid and chebulinic acid in each extract were tested by high performance liquid chromatography. The extraction steps include:

[0141] Precisely weigh 2 g of young fruit powder of Terminalia chebula Retz. that has passed through a 24-mesh sieve, add 200 mL of water, an ethanol solution with a mass percentage concentration of 65%, and an ethanol solution with a mass percentage concentration of 75%, heat under reflux for 1 hour, filter the extract, concentrate it, and freeze-dry the concentrated solution to obtain the extract of Terminalia chebula Retz. The contents of gallic acid and chebulinic acid were tested by high performance liquid chromatography. The results are shown in Table 2.

[0142] Table 2 Contents of active ingredients in extracts of each solvent

[0143]

[0144] As can be seen from the data in Table 2, the total content of gallic acid and chebulinic acid in the extract of Terminalia chebula Retz. obtained by ethanol extraction is more than 30% higher than the total content of the extract obtained by water extraction. Among them, the total content of the extract of Terminalia chebula Retz. obtained by 65% ethanol extraction is higher, and the total extraction rate is as high as 91%.

[0145] Example 3: Purification of the extract of Terminalia chebula Retz.

[0146] In this example, the young fruits of Terminalia chebula Retz. were used as raw materials, and a crude extract of Terminalia chebula Retz. was obtained by extraction with 65% ethanol, and then different types of macroporous resins were used for purification to obtain the extract of Terminalia chebula Retz.

[0147] 3.1 Preparation of the crude extract of Terminalia chebula Retz.

[0148] Solvent extraction: Take 302.56 g of raw young fruits of Terminalia chebula Retz. (not pulverized), add 6 times (feeding amount mass KG / solvent volume L) of an ethanol solution with a mass percentage concentration of 65%, heat under reflux for 2 hours, extract 2 times, combine the two extracts, filter, and concentrate to obtain 458.95 g of the crude extract of Terminalia chebula Retz. The crude extract contains 8 mg / ml of gallic acid and 86.38 mg / ml of chebulinic acid.

[0149] 3.2 Purification of the crude extract of Terminalia chebula Retz. with a single type of macroporous resin

[0150] Purification: Take the crude extract of Terminalia chebula Retz. prepared in 3.1, dilute it with water at a ratio of 1:1, and load it onto macroporous resins of types D101, HPD300, HPD100, AB-8, HP20, S-8, and NKA-9 (the resin dosage is 4 times the total amount of the crude extract). First, wash it with water, with a water washing volume of 2.5 BV, and then elute it with an ethanol solution with a mass percentage concentration of 65%, with an ethanol elution volume of 4 BV. Collect the ethanol eluate and freeze-dry it to obtain the extract of Terminalia chebula Retz. Use high-performance liquid chromatography to test the contents of gallic acid and chebulinic acid.

[0151] Table 3 Data on the purification of single-type macroporous resins

[0152]

[0153] Note: Adsorption rate = (amount of active substances in the extract before loading - amount of active substances in the loaded sample and water eluate) / amount of active substances in the extract before loading * 100%

[0154] Desorption rate = active substances desorbed by ethanol / (amount of active substances in the extract before loading - amount of active substances in the loaded sample and water eluate)

[0155] It can be seen from the data in Table 3 that the transfer rate and recovery rate of gallic acid and chebulinic acid in the extract of Terminalia chebula Retz. purified by using macroporous resins S-8 and NKA-9 alone are the highest. Therefore, these two macroporous resins are considered as the preferred purification resins for the follow-up.

[0156] 3.3 Purification of the crude extract of Terminalia chebula Retz. with combined macroporous resins

[0157] Take the crude extract of Terminalia chebula Retz. of the same batch prepared in 3.1, dilute the crude extract with water at a ratio of 1:1, and load it onto combined macroporous resins of types S-8:NKA-9 = 1:4, S-8:NKA-9 = 1:1, and S-8:NKA-9 = 4:1 (the total dosage of the mixed resins is 4 times the total amount of the crude extract) for the elution procedure.

[0158] Elution procedure: First, wash it with water, with a water washing volume of 2.5 BV, collect the water wash solution 1, and then elute it with an ethanol solution with a mass percentage concentration of 65%, with an ethanol elution volume of 4 BV, and collect the ethanol eluate 1;

[0159] Take the water wash 1, first wash it with water, with a water washing volume of 2.5 BV, collect the water wash solution 2, and then elute it with an ethanol solution with a mass percentage concentration of 65%, with an ethanol elution volume of 4 BV, and collect the ethanol eluate 2;

[0160] Take the water wash solution 2, first wash it with water, with a water washing volume of 2.5 BV, and then elute it with an ethanol solution with a mass percentage concentration of 65%, with an ethanol elution volume of 4 BV, and collect the ethanol eluate 3.

[0161] The analytical solution was collected 3 times and freeze-dried to obtain the Terminalia chebula extract. The contents of gallic acid and chebulinic acid were tested by high performance liquid chromatography.

[0162] Table 4 Data of purification by combined macroporous resin

[0163]

[0164] As can be seen from the data in Table 4, after the crude extract of Terminalia chebula extract was purified using the combination of two macroporous resins, S-8 and NKA-9, the transfer rate and yield of gallic acid and chebulinic acid in the obtained Terminalia chebula extract were significantly improved. Among them, the transfer rate sum was above 91%, and the yield was increased to above 26%. Specifically, when using S-8 alone, the highest transfer rate sum was 78.29%, and when using NKA-9 alone, the highest transfer rate sum was 83.49%. However, when the two resins were combined in equal proportion (1:1), the transfer rate sum unexpectedly increased. The transfer rate sum was 91.09% > 78.29% / 2 + 83.49% / 2 = 80.89%. The purification result after combination was significantly higher than the simple superposition of the two. The same conclusion can also be drawn in terms of yield. The yield of the combined macroporous resin was significantly higher than the simple superposition of the two effects. The HPLC chromatogram of the Terminalia chebula extract obtained from Example 3-3-1 is as Figure 1 shown.

[0165] The components, peak emergence time and their contents of the Terminalia chebula extract are shown in the following table:

[0166] Table 5 Components, peak emergence time and their contents in Terminalia chebula extract

[0167]

[0168] Example 4: Evaluation experiment on oil control efficacy

[0169] In this example, the Terminalia chebula extract prepared in Example 3-3-1 was taken, and the oil control efficacy was evaluated by taking the inhibition rate of 5α-reductase as an index.

[0170] 4.1 Positive control

[0171] Finasteride solution was used as the positive control, and the test concentration was 0.93 mg / ml.

[0172] 4.2 Experimental procedure

[0173] 4.2.1 Sample treatment: Weigh 0.2028, 0.1995, and 0.2018 g of Terminalia chebula extract samples respectively and make up the volume to 10 mL with PBS solution;

[0174] 4.2.2 Sample concentration: 20 mg / mL.

[0175] 4.2.3 Sampling process:

[0176] Sample tube A: Take 1 mL of the sample solution in a test tube, add 1 mL of 5α-reductase solution, add 1 mL of NADPH solution, add 1 mL of testosterone solution, and mix well;

[0177] Sample background tube B: Take 1 mL of the sample solution in a test tube, add 1 mL of 5α-reductase solution, add 1 mL of NADPH solution, add 1 mL of PBS solution, and mix well;

[0178] Solvent tube C: Add 1 mL of 5α-reductase solution, add 1 mL of NADPH solution, add 1 mL of testosterone solution, add 1 mL of testosterone solution, and mix well;

[0179] Solvent background tube D: Add 1 mL of 5α-reductase solution, add 1 mL of NADPH solution, add 2 mL of PBS solution, and mix well;

[0180] React each reaction solution at 37 °C for 20 minutes, transfer an appropriate amount of the sample solution, and measure the absorbance at 340 nm.

[0181] 4.3 Calculation

[0182] Calculate the inhibitory ability of the test sample on 5α-reductase and calculate the statistical difference P value to determine whether the sample has oil control efficacy.

[0183] Calculation formula: Inhibition rate (%) =

[0184] Sample A: Represents the absorbance of the sample tube;

[0185] Sample substrate B: Represents the absorbance of the sample background tube;

[0186] C: Represents the average absorbance of the solvent tube;

[0187] D: Represents the absorbance of the solvent background tube.

[0188] 4.4 Results

[0189] Table 6 Inhibition rate test result table

[0190]

[0191] The results of this test are characterized by the inhibition rate of the sample on 5α-reductase. When compared with the control substance with oil control efficacy, if the inhibition rate of the positive control substance is higher than that of the blank control group and the statistical difference value (P < 0.05), the test results are valid. If the inhibition rate of the test sample is higher than that of the blank control group and the statistical difference value (P < 0.05), it indicates that the test sample has oil control efficacy.

[0192] From Table 6 andFigure 2 It can be seen that the inhibition rates of the three groups of parallel positive control groups on 5α-reductase are higher than those of the blank control group and the statistical difference value (P<0.05), indicating that the results of this experiment are valid. The inhibition rate of the Terminalia chebula extract of this application as the experimental sample group on 5α-reductase is higher than that of the blank control group and the statistical difference value (P<0.05). The average inhibition rate of the three experimental sample groups reaches 39.82%, proving that the Terminalia chebula extract has the effect of controlling oil.

[0193] Example 5: Sunscreen effect test of Terminalia chebula extract

[0194] In this example, the Terminalia chebula extract prepared in Example 3-3-1 was taken to verify the sunscreen effect.

[0195] 5.1 Testing instruments

[0196] Sun protection factor tester SPF-290AS

[0197] 5.2 Testing environment

[0198] Environmental requirements: Temperature: 25.0°C ± 5°C; Humidity: 50% ± 10%.

[0199] 5.3 Testing procedure

[0200] (1) Fix the PMMA plate on the sample plate.

[0201] (2) First, measure the background curve of the blank plate, then use a pipettor to evenly apply the sample on the PMMA plate, and smear it with a finger wearing a latex finger cot, and gently and evenly apply the sample on the PMMA plate with an area of 25 cm 2 in an amount of 2 mg / cm 2 area.

[0202] (3) Place the smeared sample plate in a dark environment at a temperature of (25±5)°C and a relative humidity of (50±10)% and let it dry naturally for 20 min.

[0203] (4) Turn on the power and preheat the instrument for 30 min. After confirming normal, use the SPF-290AS analyzer to select 6 points on the PMMA plate for testing, and use software for calculating and analyzing the 6 light wavelength MPFs to obtain the SPF value and standard deviation of one test, and the sunscreen index SPF values of each scanning point.

[0204] (5) Each sample should be coated and tested on the plate at least 3 times.

[0205] 5.4 Judgment criteria

[0206] The Sun Protection Factor (SPF) represents the ability of a product to defend against medium-wave ultraviolet rays in specific numerical values and is a protection index for evaluating the ability of sunscreen cosmetics to protect the skin from sunburn erythema / sunburn. The larger the SPF value, the better the effect of preventing sunburn erythema / sunburn, indicating that the sunscreen product can extend the time that the skin is sunburned by UVB. Generally, sunscreen products with an SPF value between 2 and 6 are considered low-grade sunscreen products, while products with an SPF value between 6 and 8 are considered medium sunscreen products.

[0207] The test standards for other sunscreen effects are shown in Tables 7 and 8.

[0208] Table 7 Sunscreen Standard 1

[0209]

[0210] Table 8 Sunscreen Standard 2

[0211]

[0212] PA is a standard for measuring the protection ability of sunscreen products against UVA (long-wave ultraviolet rays). UVA is the main cause of skin aging and sunburn.

[0213] 5.5 Test Results

[0214] Table 9 Sunscreen Test Data

[0215]

[0216] From the data in Table 9, it can be seen that the SPF value of the Terminalia chebula extract sunscreen index of this application is 5.67, which is a low-grade sunscreen product. The measured value of the UVA PFA value is 2.01, and the PA value is +. It indicates that the Terminalia chebula extract of this application has a certain sunscreen effect, which is prominent among pure plant extract series sunscreen products.

[0217] Example 6: Experiment on the Effect of Terminalia chebula Extract on Melanin Secretion by Melanoma Cells

[0218] In this example, the Terminalia chebula extract prepared in Example 3-3-1 was taken for an experiment on the effect of mouse B16-F10 melanoma cells on melanin secretion.

[0219] 6.1 Determination of the Maximum Safe Concentration of Terminalia chebula Extract by MTT Method

[0220] 1) Inoculation: When the cell density ≥ 70%, discard the old culture medium, collect the cells, mix well and transfer them to a 2 mL centrifuge tube, centrifuge, and discard the supernatant; re-add 1 mL of complete culture medium, pipette and mix well, dilute by an appropriate multiple and count, adjust the cell density to a certain concentration according to the counting result, and inoculate the cells into a 96-well plate at a volume of 200 μL per well, then place it back in the incubator for incubation (37 °C, 5% CO2).

[0221] 2) Experimental grouping: Set up a blank control group and an experimental group in the experiment, and set another 3 cell-free wells as zero-adjustment wells. Set 6 concentration gradients for each sample in the experimental group, and set 3 replicates for each concentration gradient;

[0222] 3) Solution preparation: According to the experimental design in Table 10, prepare test substances with different concentrations using the basal medium.

[0223] Table 10 Concentration settings of Terminalia chebula Retz. extracts

[0224]

[0225] 4) Drug administration: Take out the 96-well plate after 24 h, discard the old culture medium, add 200 μL of basal medium to each well of the zero-adjustment wells and the blank control group, add 200 μL of the sample prepared with the basal medium to each well of the experimental group, and plate according to 3 replicates for each concentration in each group. Then place it back in the incubator for culture (37 °C, 5% CO2).

[0226] 5) Detection: After 24 h of drug treatment, take out the 96-well plate, add 20 μL of MTT working solution (5 mg / mL) to each well, place it back in the incubator for continued culture for 4 h, then discard the liquid in the wells, re-add 150 μL of DMSO to each well, shake for 10 min, and measure the absorbance (OD value) at a wavelength of 490 nm.

[0227] 6) Calculation of relative cell viability: Cell viability % = (OD value of the experimental group - OD value of the zero-adjustment well) / (OD value of the blank control group - OD value of the zero-adjustment group) * 100%.

[0228] 6.2 Experiment on the effect of Terminalia chebula Retz. extracts on melanin secretion of mouse B16-F10 melanoma cells

[0229] 6.2.1 Experimental design

[0230] Conduct the experimental design according to Table 11

[0231] Table 11 Experimental design for the effect of melanoma cells on melanin secretion

[0232]

[0233] 6.2.2 Experimental procedure

[0234] 1) Inoculation: When the cell density ≥ 70%, discard the old culture medium, collect the cells, mix well and transfer them to a 2 mL centrifuge tube, centrifuge, and discard the supernatant; add 1 mL of complete culture medium again, pipette and mix well, dilute by an appropriate multiple and count, adjust the cell density to a certain concentration according to the counting result, inoculate the cells into a 6-well plate at a volume of 2 mL per well, and place it back in the incubator for incubation (37 °C, 5% CO2).

[0235] 2) Experimental grouping: Group according to Table 11, and plate according to 3 replicates per concentration per group;

[0236] 3) Solution preparation: According to the cell viability test results obtained in 6.1, select an appropriate concentration as the concentration to be tested for the experimental group samples; use kojic acid (100 μg / mL) as the positive control for the experiment.

[0237] 4) Drug administration: After 24 h, take out the 6-well plate, discard the old culture medium, wash the cells with PBS, and then add samples according to 3 replicates per concentration per group. For the negative control group (NC), add 2 mL of basal medium to each of the 3 replicates. For the positive control group (PC), add 2 mL of kojic acid (100 μg / mL) to each well. For each concentration of the experimental group, add 2 mL of the corresponding concentration sample to each of the 3 replicates, and then place it back in the incubator for co-culture (37 °C, 5% CO2).

[0238] 5) Collection of melanocytes: After 24 h of drug treatment, take out the 6-well plate, discard the supernatant, collect the cells into a 2 ml EP tube, centrifuge, and discard the supernatant. Add 200 μL of 1 mol / L sodium hydroxide solution to each tube, place it in a water bath at 80 °C for 1 h, vortex and mix well. Take 150 μL from each well and transfer it to a 96-well plate. Use 1 mol / L sodium hydroxide solution as the blank, and measure the absorbance value (OD) at a wavelength of 405 nm.

[0239] 6) Result calculation: Melanin content = (OD value of the experimental group - OD value of the blank well) / (OD value of the negative control group - OD value of the blank well) * 100%.

[0240] 6.3. Experimental result collation and analysis

[0241] 6.3.1 Determination of the maximum safe concentration of Terminalia chebula Retz. extract by MTT method

[0242] Table 12 Test data of the maximum safe concentration of Terminalia chebula Retz. extract

[0243]

[0244] As shown in Table 12 and Figure 3 when the concentration of Terminalia chebula Retz. extract ≤ 125.0 μg / ml, the cell viability ≥ 90%. Therefore, an appropriate concentration can be selected within the range of ≤ 125.0 μg / ml for preparing the composition.

[0245] 6.3.2 Experiment on the Effect of Terminalia chebula Extract on Melanin Secretion by Mouse B16-F10 Melanoma Cells

[0246] The test results are as Figure 4 shown: Compared with the negative control group (NC), the melanin content secreted by cells treated with the positive control group (kojic acid) decreased significantly (*P < 0.05), indicating that the experimental conditions were effective in this experiment; compared with the negative control group (NC), the melanin content secreted by cells treated with Terminalia chebula extract at 100 μg / mL decreased significantly (*P < 0.05), and the difference was statistically significant.

[0247] The reduction amplitude of the melanin content secreted by cells treated with Terminalia chebula extract at 100 μg / mL was comparable to that of the positive control (kojic acid at 100 μg / mL), proving that the Terminalia chebula extract described in this application has the effect of inhibiting melanin secretion.

[0248] Example 7: Experiment on the Effect of Terminalia chebula Extract on Streptococcus mutans and Porphyromonas gingivalis

[0249] In this example, the Terminalia chebula extract prepared in Example 3-3-1 was taken for the experiment on the effect on Streptococcus mutans and Porphyromonas gingivalis.

[0250] 7.1 Test Equipment

[0251] (1) Strains

[0252] Streptococcus mutans BNCC 336931 and Porphyromonas gingivalis ATCC 33277

[0253] (2) Hydrolyzed casein agar medium (MH), weigh 38 g of MH agar medium, dissolve it in 1000 ml of water, heat it to boiling for dissolution, then autoclave at 121 °C for 15 min, and place it in a water bath at 45 °C - 50 °C for standby. This medium will be used for the control test.

[0254] (3) 0.03 mol / L phosphate buffer solution with pH 7.2.

[0255] (4) Pipettor (1 μl - 10 μl).

[0256] (5) Water bath incubator at 45 °C - 50 °C.

[0257] (6) Pipettes, test tubes, petri dishes.

[0258] (7) Incubator at 37 °C.

[0259] 7.2 Operating Procedures

[0260] (1) Preparation of Streptococcus mutans and Porphyromonas gingivalis solutions: Take 5 ml or 5 g (after solid grinding) of the sample under aseptic conditions and place it in 45 ml of sterilized phosphate buffer. Vigorously shake and dissolve to prepare a 10% uniformly dispersed solution or suspension.

[0261] (2) Preparation of antibacterial agent-containing medium: Dilute the prepared 10% antibacterial solution or suspension in a series of two-fold dilutions with PBS to obtain test solutions of different concentrations, and keep them at a constant temperature in a water bath at 45°C - 50°C for later use.

[0262] (3) Preparation of double-concentration medium: Weigh 76 g of MH agar medium and dissolve it in 1000 ml of water. Heat to boiling for dissolution. Then sterilize it at 121°C under pressure steam for 15 min and keep it at a water bath at 45°C - 50°C for later use. This medium will be used to dilute the antibacterial solution or suspension.

[0263] (4) Preparation of medium containing antibacterial solution: Take 10 ml of the serially diluted antibacterial solution and add it into a petri dish respectively. Add 10 ml of the double-concentration MH agar medium in the water bath at 45°C - 50°C into the petri dish, and shake the petri dish while adding to fully mix the antibacterial solution and the medium. Wait for it to solidify and then keep it for later use.

[0264] (5) Use a pipette to take 1 μl - 2 μl (bacterial content is about 107 cfu / ml) of the bacterial suspension and spot-seed it on the petri dish containing the antibacterial (or inhibitory) solution medium. The diameter of the bacterial solution circle formed after inoculation is about 5 mm - 8 mm (the bacterial content of each spot is about 104 cfu).

[0265] (6) Inoculate the MH agar plate without antibacterial (or inhibitory) components in the same way as a positive control.

[0266] (7) Place the inoculated plate in an incubator at 35°C and incubate it upside down for 18 h - 24 h to observe the results.

[0267] 7.3 Judgment regulations

[0268] The lowest antibacterial solution concentration at which colony growth is completely inhibited is the MIC of the sample against the test bacteria. The growth of a single colony can be ignored.

[0269] 7.4 Experimental results

[0270] Table 13 Experimental data on the effects of Terminalia chebula Retz. extracts on Streptococcus mutans and Porphyromonas gingivalis

[0271]

[0272] From the data in Table 13, it can be seen that the Terminalia chebula Retz. extracts of this application have inhibitory effects on Streptococcus mutans and Porphyromonas gingivalis, and the minimum inhibitory concentrations (MICs) are 0.312 wt% and 0.078 wt% respectively.

[0273] All documents mentioned in this invention are cited herein as references, as if each document was individually cited as a reference. In addition, it should be understood that after reading the above teachings of this invention, those skilled in the art can make various changes or modifications to this invention, and these equivalent forms also fall within the scope defined by the appended claims of this application.

Claims

1. A Terminalia chebula extract, characterized in that, The Terminalia chebula extract contains gallic acid and chebulinic acid, and the total content of the gallic acid and chebulinic acid > 20 wt%, calculated based on the dry weight of the Terminalia chebula extract; Among them, the Terminalia chebula extract is prepared by the following method: (1) Extraction: The Terminalia chebula fruits are extracted with ethanol, the filtrate is collected and concentrated to obtain a crude extract of Terminalia chebula extract; (2) Purification: Take the crude extract obtained in step (1), and elute it with a combined macroporous resin of S-8 and NKA-9, and the elution includes: first elute with water, collect the water eluate, and then elute with ethanol, collect the ethanol eluate; (3) Take the water eluate obtained in step (2), use ethanol as the eluent, and continue to elute with a combined macroporous resin of S-8 and NKA-9 to obtain an ethanol eluate; (4) Combine the ethanol eluates obtained in steps (2) and (3), and lyophilize to obtain the Terminalia chebula extract.

2. The Terminalia chebula extract according to claim 1, wherein The extract also includes chebulagic acid and / or ellagic acid, wherein the content of chebulagic acid is 10 - 20 wt%, and / or the content of ellagic acid is 1 - 10 wt%.

3. The preparation method of the Terminalia chebula extract according to claim 1, characterized in that, The method includes the steps: (1) Extraction: The Terminalia chebula fruits are extracted with ethanol, the filtrate is collected and concentrated to obtain a crude extract of Terminalia chebula extract; (2) Purification: Take the crude extract obtained in step (1), and elute it with a combined macroporous resin of S-8 and NKA-9, and the elution includes: first elute with water, collect the water eluate, and then elute with ethanol, collect the ethanol eluate; (3) Take the water eluate obtained in step (2), use ethanol as the eluent, and continue to elute with a combined macroporous resin of S-8 and NKA-9 to obtain an ethanol eluate; (4) Combine the ethanol eluates obtained in steps (2) and (3), and lyophilize to obtain the Terminalia chebula extract.

4. The preparation method of the Terminalia chebula extract according to claim 1, characterized in that, The method includes the steps: (1) Extraction: Provide the raw material of Terminalia chebula fruits, add ethanol with a concentration of 60 v / v % - 70 v / v % for reflux extraction, collect the filtrate, and concentrate to obtain a crude extract of Terminalia chebula extract; (2) Purification: Load the crude extract obtained in step (1) onto a combined macroporous resin of S-8 and NKA-9, and perform an elution procedure: first elute with water, collect the water eluate, and then elute with ethanol with a concentration of 60 v / v % - 70 v / v %, collect the ethanol eluate; (3) Take the water eluate obtained in step (2), repeat the elution procedure in step (2) 2 - 4 times, and combine the ethanol eluates obtained each time; (4) Take the ethanol eluate obtained in step (3), and lyophilize to obtain the Terminalia chebula extract.

5. The method according to claim 3, wherein In step (2), the elution procedure is repeated 3 times, and the elution procedure includes the steps: (2 - 1) First elute with water, collect the water eluate 1, and then elute with ethanol with a concentration of 60 v / v % - 70 v / v %, collect the ethanol eluate 1; (2 - 2) Take the water eluate 1 obtained in step (2 - 1), first elute with water, collect the water eluate 2, and then elute with ethanol with a concentration of 60 v / v % - 70 v / v %, collect the ethanol eluate 2; (2-3) Take the washing liquid 2 obtained in step (2-1), first elute with water, and then elute with ethanol at a concentration of 60 v / v % - 70 v / v %, collect the ethanol eluate 3, and combine the ethanol eluates obtained each time.

6. The method according to claim 3, wherein The Terminalia chebula fruits described in step (1) are Terminalia chebula immature fruits or Terminalia chebula mature fruits.

7. The method according to claim 3, wherein In step (2), the resin dosage of the combined macroporous resin is S-8:NKA-9 = 10:1 - 10:1 (w:w).

8. The use of the Terminalia chebula extract according to claim 1, characterized in that, For preparing daily-use products with oil control, sun protection, whitening or bacteriostatic effects.

9. Use of the Terminalia chebula extract according to claim 1, characterized in that, For preparing daily chemical products with oil control, sun protection, whitening or bacteriostatic effects.

10. The use according to claim 8 or 9, characterized in that, The said product also has one or several characteristics selected from the following group: (1) For inhibiting 5α-reductase; (2) The sun protection factor SPF value ≥ 5.5; (3) Reducing the melanin content: (4) Inhibiting microorganisms selected from the following group: Streptococcus mutans and Porphyromonas gingivalis.

11. A composition, characterized in that, The said composition includes: (1) the Terminalia chebula extract described in claim 1; and (2) additional components that can be used in daily chemical products.

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