Ganoderma lucidum spore powder with savoury, mellow and smooth taste and preparation method thereof

By performing short-term impact freezing and low-temperature grinding of Ganoderma lucidum spore powder before breaking the wall, combined with inert gas protection and high-barrier packaging, the problems of oxidation, rancidity and flavor loss in traditional wall breaking methods are solved, and the effects of efficient wall breaking, active ingredient retention and mellow and silky taste are achieved.

CN119868420AInactive Publication Date: 2025-04-25ZHEJIANG BAIKANG PHARM CO LTD

Patent Information

Application Number
CN202510372076.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-27
Publication Date
2025-04-25
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

When grinding the spore powder of Ganoderma lucidum in traditional high temperature or at room temperature, it is easy to cause oxidation and rancidity, burnt and flavor loss, and low storage rate of active ingredients, making it difficult to achieve the best balance between high wall breakage, high activity retention and no odor taste.

Method used

Short-term impact freezing technology is used to quickly embrittle the spore walls at extremely low temperatures of -60℃, and inert gas protection and high-barrier packaging are combined in subsequent low-temperature grinding, ultrafine powder and nitrogen filling packaging to maximize the preservation of active ingredients and enhance the taste.

Benefits of technology

Significantly improve wall breaking efficiency, reduce energy consumption, effectively avoid loss of thermally sensitized active ingredients, reduce the risk of oxidation and rancidity, improve the stability of taste and flavor, and significantly extend the shelf life of the product.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention belongs to the technical field of traditional Chinese medicine decoction piece processing, and particularly relates to ganoderma lucidum spore powder with fragrant, mellow and smooth taste and a preparation method of the ganoderma lucidum spore powder. According to the method, short-time impact freezing treatment is added before wall breaking of the ganoderma lucidum spore powder, rapid freezing and embrittlement of spore walls are achieved in an extremely low-temperature environment, mechanical energy needed in subsequent low-temperature grinding is greatly reduced, and therefore the grinding heat generation and oxidation risk are effectively reduced. In cooperation with strict inert gas protection and high-barrier packaging, active ingredients of the ganoderma lucidum spore powder can be reserved to the maximum extent, the savoury and mellow degree and the smooth taste of the ganoderma lucidum spore powder are remarkably improved, and the shelf life of the product is further prolonged.
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Description

Technical Field

[0001] The invention belongs to the technical field of processing Chinese medicinal pieces, and in particular relates to ganoderma lucidum spore powder with a mellow and smooth taste and a preparation method thereof. Background Art

[0002] Ganoderma lucidum spore powder is the spore particles ejected by Ganoderma lucidum in the mature stage, which is rich in various active ingredients, such as triterpenes, polysaccharides and unique Ganoderma lucidum spore oil. Due to the thick wall of Ganoderma lucidum spores, its nutritional value and medicinal value cannot be fully exerted if the wall is not effectively broken. However, in the traditional high temperature or room temperature grinding process of breaking the wall, the following problems often exist: 1. Oxidation and rancidity: When the oil contained in the spore powder of Ganoderma lucidum is exposed to high temperature, air or humidity changes, it is easy to be oxidized and decomposed, producing a smell of wormwood or sourness, or discoloring or deteriorating. At the same time, heat-sensitive active substances such as triterpenes and polysaccharides are also prone to degradation or inactivation when the cell wall is broken.

[0003] 2. Burnt smell and flavor loss: When the wall breaking machine runs at high speed, local high temperature will be generated in the grinding chamber, which will cause some oil and protein to burn and produce odor, which not only destroys the natural mushroom aroma of Ganoderma lucidum, but also reduces the taste quality of the product.

[0004] 3. The preservation rate of active ingredients is relatively low: Affected by high temperature, oxidation and moisture, if there is a lack of low-temperature protection or effective oxygen isolation measures during the wall-breaking process of Ganoderma lucidum spore powder, the content of active ingredients will drop significantly after wall-breaking, making it difficult to maintain a long shelf life.

[0005] In response to the above problems, some existing technologies have proposed methods of breaking the spores in a low-temperature environment or filling and packaging with nitrogen after breaking the spores to reduce the risk of oxidation. However, these methods are still insufficient in terms of the temperature connection between the front-end pretreatment and the breaking process: if the Ganoderma lucidum spore powder is simply precooled to -20°C or higher and then broken by conventional methods, it is still difficult to suppress the heat accumulation caused by mechanical friction, and it is not enough to quickly embrittle the spore wall, resulting in unsatisfactory breaking efficiency or activity retention rate.

[0006] "Short-time shock freezing" or "flash freezing" technology is often used in the preservation of food or biological products. It uses extremely low temperatures (such as -60°C or even lower) to quickly freeze the cell tissue of the material in a very short time, reducing the secondary damage to the cell structure caused by the crystallization of water molecules, and achieving a high level of protection for heat-sensitive active ingredients. However, there is still a lack of mature integrated solutions for the systematic application of short-time shock freezing technology to the deep wall breaking process of Ganoderma lucidum spore powder. How to achieve rapid embrittlement of spore powder at an extremely low temperature of -60°C and closely connect it with subsequent low-temperature grinding, ultrafine powder and nitrogen-filled packaging processes is the key to finding the best balance between high wall breaking rate, high activity retention and odor-free taste of Ganoderma lucidum spore powder. Summary of the invention

[0007] In order to solve the above technical problems, the purpose of the present invention is to provide a Ganoderma lucidum spore powder with a mellow and smooth taste and a preparation method thereof, by adding a "short-time impact freezing" treatment before the Ganoderma lucidum spore powder is broken, the spore wall is quickly frozen and embrittled in an extremely low temperature environment, so that the mechanical energy required for subsequent low-temperature grinding is greatly reduced, thereby effectively reducing the generation of grinding heat and oxidation risks. Combined with strict inert gas protection and high-barrier packaging, the active ingredients of Ganoderma lucidum spore powder can be retained to the maximum extent, significantly improving its mellowness and silky taste, and further extending the shelf life of the product.

[0008] In order to achieve the above-mentioned purpose, the present invention adopts the following technical solutions: A method for preparing ganoderma lucidum spore powder with a mellow and smooth taste, the method comprising the following steps: 1) Raw material pretreatment: We use high-quality Ganoderma lucidum spore powder that imitates wild organic cultivation, and pre-freeze the spore powder in an environment of -20 to -25℃ to reduce the moisture content to 5%~8%; 2) Deep frozen extraction and wall breaking: The pre-frozen Ganoderma lucidum spore powder is subjected to a short-term shock freezing treatment at -60 to -80°C for 10 to 30 minutes to make the spore powder cell wall quickly brittle in an extremely low temperature environment; 3) Low temperature grinding and wall breaking The impact-frozen spore powder is then transferred to a grinding chamber with a temperature no higher than -5°C for cryogenic grinding and wall breaking. The grinding temperature is controlled in the range of -5°C to 0°C through a real-time temperature monitoring system, and the wall breaking rate reaches more than 99%. The particle size is controlled in the range of 5~15μm through a sieve of 800 mesh or above or laser particle size detection. 4) Real-time inert gas protection: During the above-mentioned wall breaking and ultrafine powder process, high-purity nitrogen or inert gas is continuously introduced to reduce the oxygen content to less than 2%, and the vacuum or nitrogen-filled packaging is completed within 1 hour.

[0009] Preferably, the pretreatment is to place the spore powder in a -20°C environment for pre-freezing treatment to reduce the moisture content to 6% to 7%.

[0010] Preferably, during the short-time shock freezing process, the temperature is controlled at -65 to -75°C for 15-20 minutes, and during this process the Ganoderma lucidum spore powder is spread out in layers with a thickness not exceeding 2 cm to ensure that the spore powder is quickly cooled to below -60°C.

[0011] Preferably, during low-temperature grinding and wall breaking, the rotation speed of the grinder is controlled within the range of 500-1000 rpm, and an intermittent grinding mode is adopted, with each grinding not exceeding 2 minutes, and the machine is stopped for 10-20 seconds to cool down, so as to stably maintain the temperature in the bin at -5°C~0°C.

[0012] Preferably, the high-purity nitrogen is nitrogen with a purity of ≥99.9%, and independent nitrogen delivery pipelines are provided in the grinding bin, screening bin and packaging bin to maintain the oxygen content ≤2% in different production links.

[0013] Preferably, the packaging material used for packaging is an aluminum foil composite film or an aluminum-coated film, whose oxygen permeability is not higher than 0.5 cm³ / (m²·24h·0.1MPa) and water vapor permeability is not higher than 0.5 g / (m²·24h).

[0014] Preferably, within 1 hour after the cell wall breaking is completed, the obtained Ganoderma lucidum spore powder is packaged into vacuum or nitrogen-filled packaging bags of 100-500 g / bag to reduce the risk of oxidation during single unsealing.

[0015] Furthermore, the present invention also provides Ganoderma lucidum spore powder obtained by the preparation method, wherein the Ganoderma lucidum spore powder has natural mushroom fragrance, is delicate and smooth in the mouth, and has no wormwood smell, sour smell or burnt smell caused by oil rancidity.

[0016] Preferably, the Ganoderma lucidum spore powder meets the following conditions: Particle size distribution is 5 to 15 μm; The spore wall breaking rate reaches or exceeds 99%; The peroxide value of the product is not higher than 0.02g / 100g; The moisture content is controlled between 5% and 8%.

[0017] Preferably, after impact freezing and cryogenic grinding, the peroxide value of the spore powder does not exceed 0.02 g / 100g, and after storage at room temperature for 24 months, the retention rate of the active ingredients triterpenes and polysaccharides is higher than 90%.

[0018] The present invention has the following technical effects due to the adoption of the above technical solution: 1. Significantly improve the wall breaking efficiency and reduce energy consumption: By short-term impact freezing of Ganoderma lucidum spore powder at -60°C or lower, the spore wall is quickly brittle. Since the spore wall becomes easy to break, the mechanical energy required in the subsequent low-temperature grinding stage is significantly reduced, and a high wall breaking rate of more than 99% can be achieved under low speed or intermittent grinding conditions. Compared with traditional pre-cooling treatment at room temperature or only -20°C, the process of the present invention can shorten the grinding time and reduce power consumption at the same production capacity, while avoiding excessive temperature rise in the grinding chamber.

[0019] 2. Effectively avoid the loss of heat-sensitive active ingredients: Ganoderma lucidum spore powder is rich in active substances such as triterpenes, polysaccharides and Ganoderma lucidum spore oil, which are extremely sensitive to temperature and oxidation. By first short-term shock freezing and completing the grinding in a low-temperature environment of -5℃~0℃, the burnt smell and degradation of active ingredients caused by high temperature are avoided. Experimental tests show that after the process of the present invention, the retention rate of key ingredients such as triterpenes and polysaccharides is increased by more than 10% compared with the normal temperature grinding technology, and the degree of oil oxidation is significantly reduced.

[0020] 3. Significantly reduce the risk of oxidation and rancidity: The process of short-term shock freezing combined with low-temperature wall breaking can inhibit oil rancidity and microbial growth to the maximum extent. The simultaneous inert gas protection (such as nitrogen or carbon dioxide) further reduces the chance of oxidation during the wall breaking and ultrafine powder process. Nitrogen filling and packaging are carried out within 1 to 2 hours after the wall breaking is completed, and high-barrier packaging materials are used, which can continuously maintain indicators such as peroxide value and acid value at a low level and significantly extend the shelf life of the product.

[0021] 4. Improve the stability of taste and flavor: After the spore wall is brittle at extremely low temperature through short-term impact freezing, it does not need to be ground at high speed for a long time, thereby reducing the burnt smell and flavor loss caused by friction heat. The Ganoderma lucidum spore powder treated by the process of the present invention can maintain the unique natural mushroom fragrance of Ganoderma lucidum, and after ultra-fine grinding (particle size of about 5-15μm), it presents an excellent taste of delicate, silky and non-granular taste.

[0022] 5. Significantly extend the shelf life and ensure quality consistency: After short-term shock freezing, low-temperature wall breaking, and nitrogen-filled packaging, the activity indicators of triterpenes, polysaccharides, etc. of the Ganoderma lucidum spore powder have basically not decreased significantly within 24 months under room temperature or low-temperature storage conditions. At the same time, the oil rancidity rate and the proliferation rate of mold colonies are greatly reduced, ensuring that the product can maintain its mellow flavor and high biological activity during long-term storage.

[0023] In summary, the present invention has achieved significant improvements in reducing energy consumption, increasing the retention rate of active ingredients, enhancing taste quality and extending shelf life, overcoming the limitations of traditional room temperature wall breaking or simple low-temperature pre-cooling treatment in active ingredient protection and flavor retention, and has important application value for the processing and production of Ganoderma lucidum spore powder and other similar highly active bacterial powders. DETAILED DESCRIPTION

[0024] The following is a clear and complete description of the technical solutions in the embodiments of the present invention in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Example 1 1. Raw material preparation The high-quality spore powder of Ganoderma lucidum cultivated in simulated wild organic way is selected, which is free of impurities. After conventional micro-sieving, the spore powder with high purity is obtained. The spore powder is placed in a -20℃ environment for about 12 hours to reduce its moisture content to about 6.5%.

[0026] 2. Short-term shock freezing Take out the pre-cooled spore powder and spread it in a thin layer (no more than 2 cm thick) on a metal tray; place it in a -70℃ impact freezing device for 15 minutes, during which the surface temperature of the material is monitored in real time to ensure that it drops quickly to around -60℃ in a short time.

[0027] 3. Low temperature wall breaking The impact-frozen spore powder was quickly transferred to the grinding chamber of the wall-breaking machine, which was precooled to -5°C; the grinding machine speed was set to 800 rpm, and intermittent grinding was adopted: the machine was stopped for 10 seconds after each grinding for 1 minute, and the cycle was repeated until the wall-breaking rate reached more than 99%. In order to inhibit oxidation, nitrogen with a purity of ≥99.9% was introduced during the grinding process to ensure that the oxygen content in the chamber did not exceed 2%, and the grinding temperature was maintained in the range of -5°C to 0°C using a real-time temperature monitoring system. The particle size of the discharge was controlled at 5~15μm by an online laser particle size analyzer; an 800-mesh screen was used for final classification, and the resulting powder was uniform and fine.

[0028] 4. Nitrogen filling packaging The product should be packed within 1 hour after the cell wall breaking is completed, using high-barrier aluminum foil composite bags. After nitrogen flushing, the residual oxygen in the bag should be less than 2%. The net weight of each bag is 400 g. After sealing, the oxygen permeability should not exceed 0.5 cm³ / (m²·24h·0.1MPa), and the water vapor permeability should not exceed 0.5 g / (m²·24h).

[0029] Example 2 1. Raw material pretreatment High-quality Ganoderma lucidum spore powder collected from another production area, after being collected and removed by non-woven sleeves, has a moisture content of about 8%. It is pre-frozen for 16 hours in a -25℃ environment to reduce the moisture content to about 6%.

[0030] 2. Short-term shock freezing The pre-cooled spore powder was shock-frozen at -70°C for 20 minutes; the layer thickness was controlled at about 1.5 cm to ensure that the core temperature of the material quickly dropped to below -60°C.

[0031] 3. Low temperature grinding and wall breaking The temperature of the grinding chamber was set at -3°C, the speed of the grinder was about 600 rpm, and the working mode was intermittent. High-purity nitrogen was continuously introduced during grinding, and the oxygen content in the chamber was measured to be about 1.8% on average. After about 15 minutes, the wall breaking rate reached 99.2%, and the material was in a fine powdery state.

[0032] The detected particle size D50 is about 10μm; use an 800-mesh sieve to remove some of the oversized particles, and then mix them evenly with the finer powder.

[0033] 4. Nitrogen filling packaging Packaging is completed within 0.8 hours (about 48 minutes) after cell wall breaking; each package has a net weight of 400 g, is vacuum sealed, and the residual oxygen content is less than 1.5%.

[0034] Comparative Example 1 1. Raw materials and precooling The same batch of Ganoderma lucidum spore powder as in Example 1 was used, with a moisture content of about 8%. The powder was simply pre-cooled at -20°C for 4 hours without short-term shock freezing treatment.

[0035] 2. Breaking the wall at room temperature The grinder was set to directly break the wall at room temperature (about 25°C); the speed of the wall breaker was about 1200 rpm, and the grinding was continuous for 12 minutes, and the wall breaking rate was measured to be about 96.5%; at this time, the temperature of the grinding chamber wall rose to about 45°C, and the material had a slight burnt smell.

[0036] 3. Conventional packaging The packaging was carried out about 4 hours after the cell wall breaking was completed. No nitrogen replacement was performed and only ordinary PE plastic bags were used for sealing.

[0037] Comparative Example 2 The same batch of Ganoderma lucidum spore powder as in Example 1 was used, and no short-term shock freezing treatment was performed. Other technical features were the same as in Example 1.

[0038] The following is the test process and experimental data based on Example 1, Example 2 and Comparative Example 1 and Comparative Example 2, which is intended to demonstrate the feasibility and superiority of the process of the present invention. The data provided include wall breaking rate, peroxide value, active ingredient retention rate and taste evaluation, for reference by those skilled in the art.

[0039] 1. Detection method of cell wall breaking rate Using microscopy Instruments and materials: high-power microscope (400 times or higher); physiological saline or appropriate buffer solution; slides, coverslips, pipettes, etc.

[0040] Operation steps: Take a small amount of broken spore powder (about 0.1-0.2 g), add appropriate amount of physiological saline or buffer, and mix thoroughly to make a suspension. Pipette a drop of suspension onto a glass slide and cover it with a coverslip. Observe at least 5-10 fields of view under a microscope and record the number of broken spore particles and the number of intact unbroken spore particles.

[0041] Calculate the wall breaking rate:

[0042] Note: To ensure that the sampling is representative, it is recommended to take samples at multiple points and conduct three parallel tests. If the spores are difficult to disperse, use an appropriate amount of surfactant or ultrasonic treatment to disperse the particles and then observe. When calculating, the results of multiple fields of view should be combined and the average value should be taken as the final wall-breaking rate.

[0043] 2. Peroxide value (POV) test method, refer to national standard (GB 5009.227) Principle: Peroxide value mainly reflects the degree of oil oxidation and rancidity. The iodine titration method or titration method is usually used to detect the oxidation state of oil in Ganoderma lucidum spore powder.

[0044] Brief steps: Weigh a certain amount of Ganoderma lucidum spore powder (according to standard methods, such as 2-5 g) into a conical flask; add a mixed solvent (glacial acetic acid-n-hexane, etc.) and oscillate for extraction; add a saturated potassium iodide solution and place it away from light to release iodine; titrate the free iodine with a standard sodium thiosulfate solution; calculate the peroxide value (in g / 100 g or mmol / kg).

[0045] Note: Avoid prolonged exposure to air, light or high temperature during operation; reagent preparation and titration steps should strictly follow standard procedures; parallel tests can be performed and the average value can be taken.

[0046] 3. Active ingredient retention rate detection method The main active ingredients of Ganoderma lucidum spore powder include triterpenes, polysaccharides, Ganoderma lucidum spore oil, etc. The following is an explanation using triterpenes and polysaccharides as examples.

[0047] 3.1 Determination of triterpenoid content by HPLC Principle: Ganoderma triterpenoids are separated by HPLC and quantified by detector (UV or DAD); Steps: Weigh an appropriate amount of Ganoderma lucidum spore powder and use methanol / ethanol and other solvents for ultrasonic extraction; filter and remove impurities, then inject into HPLC for separation and detection; compare with standard substances (such as ganoderic acid A, ganoderic acid B, etc.), and calculate the total content of triterpenes.

[0048] Note: The standards, mobile phases, column temperatures, etc. used must be stable; methodological validation (accuracy, precision, linear range, etc.) should be performed.

[0049] 3.2 Determination of polysaccharide content by phenol-sulfuric acid method Principle: The polysaccharide reacts with the phenol-sulfuric acid mixed reagent to produce an orange substance, and the absorbance is measured at 490 nm; Steps: Accurately weigh the Ganoderma lucidum spore powder, and extract the polysaccharide using hot water or enzymatic hydrolysis; add phenol-sulfuric acid reagent to the extract for reaction, heat in a water bath and then cool; measure the absorbance using a spectrophotometer and compare it with the glucose standard curve for quantification.

[0050] Note: The extraction method, time, and temperature should be unified to ensure parallelism; avoid interference from proteins, pigments, etc.

[0051] 3.3 Calculation of active ingredient retention rate Taking triterpenes as an example, assuming that the triterpene content of the raw material before cell wall breaking is T0, and the triterpene content measured after cell wall breaking or after storage for N months is T N , then the triterpene retention rate can be expressed as:

[0052] Similarly, polysaccharide retention rate can also be calculated similarly.

[0053] 4. Taste evaluation method Taste tasting panel scoring method The evaluation indicators are as follows: Appearance and color: whether the powder color is uniform and whether there are burnt particles; Smell and aroma: whether the unique mushroom aroma of Ganoderma lucidum is retained, whether there is any wormwood smell, sour smell or burnt smell; Taste and graininess: Is the taste smooth and delicate, or is there any noticeable grit or impurities? Comprehensive acceptance: overall pleasure and aftertaste.

[0054] Operation process: Randomly sample about 2-3 g of Ganoderma lucidum spore powder from the same batch of products, brew it with room temperature water or warm water for tasting; a 5-10-person tasting panel (well-trained) will score each item, and the score range can be set to 0-10 points or 0-5 points; remove the highest and lowest scores and take the average value as the taste evaluation result of the sample.

[0055] Note: To reduce subjective bias, each judge should take a certain amount of time to clear the taste in their mouth; the evaluation environment should be kept as quiet as possible without any odor interference; you can also take a small amount of dry powder directly to taste its fineness.

[0056] The following data compares the differences in core indicators between the examples and the comparative examples, including wall breaking rate, peroxide value (POV), active ingredient retention rate and taste score, etc. For easy observation, they are summarized in Table 1 and Table 2.

[0057] Table 1: Comparison of initial indicators (immediate detection results after cell wall breaking and encapsulation)

[0058] Note: The content of triterpenes and polysaccharides varies slightly from batch to batch. The table shows the average values ​​of each group. For comparative example 1, there is no nitrogen packaging, and the residual oxygen measured is basically consistent with that in the atmosphere (about 21%).

[0059] Table 2: Comparison of main indicators after 24 months of storage (normal temperature 25±2℃)

[0060] Note: After 6 months of storage at room temperature, the peroxide value of comparative example 1 has reached 0.055 g / 100g, and it is obviously rancid, so only 6-month data is listed in this group; in the taste score, burnt taste and wormwood taste will lower the overall score.

[0061] The above is a description of the embodiments of the present invention. Through the above description of the disclosed embodiments, professionals and technicians in the field can implement or use the present invention. Various modifications to these embodiments will be apparent to professionals and technicians in the field. The general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to these embodiments shown in this article, but will conform to the widest range consistent with the principles and novelties disclosed herein.

Claims

1. A method for preparing Ganoderma lucidum spore powder with a mellow and smooth taste, characterized in that: The method comprises the following steps: 1) Raw material pretreatment: We use high-quality Ganoderma lucidum spore powder that imitates wild organic cultivation, and pre-freeze the spore powder in an environment of -20 to -25℃ to reduce the moisture content to 5%~8%; 2) Deep frozen extraction and wall breaking: The pre-frozen Ganoderma lucidum spore powder is subjected to a short-term shock freezing treatment at -60 to -80°C for 10 to 30 minutes to make the spore powder cell wall quickly brittle in an extremely low temperature environment; 3) Low temperature grinding and wall breaking: The impact-frozen spore powder is then transferred to a grinding chamber with a temperature no higher than -5°C for cryogenic grinding and wall breaking. The grinding temperature is controlled in the range of -5°C to 0°C through a real-time temperature monitoring system, and the wall breaking rate reaches more than 99%. The particle size is controlled in the range of 5~15μm through a sieve of 800 mesh or above or laser particle size detection. 4) Real-time inert gas protection: During the above-mentioned wall breaking and ultrafine powder process, high-purity nitrogen or inert gas is continuously introduced to reduce the oxygen content to less than 2%, and the vacuum or nitrogen-filled packaging is completed within 1 hour.

2. The preparation method according to claim 1, characterized in that: The pretreatment is to place the spore powder in a -20°C environment for pre-freezing treatment, so that the moisture content is reduced to 6% to 7%.

3. The preparation method according to claim 1, characterized in that: During the short-time shock freezing process, the temperature is controlled at -65 to -75°C for 15-20 minutes, and during this process, the Ganoderma lucidum spore powder is spread out in layers with a thickness not exceeding 2 cm to ensure that the spore powder is quickly cooled to a temperature below -60°C.

4. The preparation method according to claim 1 or 2, characterized in that: During low-temperature grinding and wall breaking, the speed of the grinder is controlled within the range of 500-1000 rpm, and an intermittent grinding mode is adopted. Each grinding does not exceed 2 minutes, and the machine is stopped for 10-20 seconds to cool down, so as to stably maintain the temperature in the chamber at -5℃~0℃.

5. The preparation method according to claim 1, characterized in that: The high-purity nitrogen is nitrogen with a purity of ≥99.9%, and independent nitrogen delivery pipelines are provided in the grinding bin, screening bin and packaging bin to maintain the oxygen content ≤2% in different production links.

6. The preparation method according to claim 1, characterized in that: The packaging material used for packaging is aluminum foil composite film or aluminum-coated film, and its oxygen permeability is not higher than 0.5 cm³ / (m²·24h·0.1MPa), and its water vapor permeability is not higher than 0.5 g / (m²·24h).

7. The preparation method according to claim 1, characterized in that: Within 1 hour after the cell wall breaking is completed, the obtained Ganoderma lucidum spore powder is packaged into vacuum or nitrogen-filled packaging bags of 100-500g / bag to reduce the risk of oxidation during single unsealing.

8. The Ganoderma lucidum spore powder obtained by the preparation method according to any one of claims 1 to 7, characterized in that: The ganoderma lucidum spore powder has a natural mushroom aroma, is delicate and smooth in the mouth, and is free of wormwood smell, sour smell or burnt smell caused by oil rancidity.

9. The Ganoderma lucidum spore powder according to claim 8, characterized in that: The Ganoderma lucidum spore powder meets the following conditions: Particle size distribution is 5 to 15 μm; The spore wall breaking rate reaches or exceeds 99%; The peroxide value of the product is not higher than 0.02g / 100g; The content of the components is controlled between 5% and 8%.

10. The Ganoderma lucidum spore powder according to claim 8, characterized in that: After impact freezing and low-temperature grinding, the peroxide value of the spore powder does not exceed 0.02 g / 100g, and after being stored at room temperature for 24 months, the retention rate of the active ingredients triterpenes and polysaccharides is higher than 90%.

Citation Information

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