A Chinese herbal medicine composition for pharyngitis and its application

Abstract

This invention provides a traditional Chinese medicine (TCM) fresh herb composition for pharyngitis and its application, relating to the field of TCM pharmaceutical technology. The TCM fresh herb composition consists of fresh Artemisia argyi leaves, fresh Asparagus cochinchinensis leaves, fresh mulberry leaves, and fresh licorice root. Through a precise combination of principal, assistant, and adjuvant herbs, it possesses the effects of clearing heat and detoxifying, moistening dryness and promoting fluid production, reducing swelling and relieving pain. Experimental data shows that the TCM fresh herb composition of this invention has significant anti-inflammatory effects, significantly improving the food and water intake, scratching frequency, and pharyngeal mucosal congestion and swelling in rats with acute pharyngitis, thus improving pharyngeal tissue lesions. This TCM fresh herb composition can be developed and utilized as a new drug for the prevention and treatment of pharyngitis.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine manufacturing technology, specifically to a composition of fresh Chinese herbal medicine for pharyngitis and its application. Background Technology

[0002] Pharyngitis is an inflammatory disease of the pharyngeal mucosa and its substructure, including clinically diagnosed pharyngitis, tonsillitis, nasopharyngitis, and other upper respiratory tract infection types. It typically presents as an acute illness, but can also be subacute or chronic. Acute pharyngitis is a common and frequently occurring disease in otolaryngology, caused by bacterial or viral infections or physical and chemical factors, affecting the pharyngeal mucosa, submucosa, and related lymphoid tissues. It is most prevalent in winter and spring, and during seasonal transitions. Clinical manifestations include cough, sore throat, hoarseness, and fever. Physical examination may reveal pharyngeal mucosal congestion, redness and swelling of the uvula, soft palate, and posterior pharyngeal wall lymphoid follicles, and enlarged submandibular or submental lymph nodes.

[0003] Inspired by the use of fresh artemisia in treating malaria recorded in the *Elbow-Side Emergency Prescriptions*, Dr. Tu Youyou discovered artemisinin, which spurred widespread research and application of fresh Chinese medicinal herbs. Fresh Chinese medicinal herbs refer to unprocessed fresh plants (including roots, stems, leaves, flowers, and fruits) and fresh live animals (including whole animals, organs, and tissues), used directly to treat diseases under the guidance of traditional Chinese medicine theory. This is a distinctive feature of traditional Chinese medicine, first mentioned in the *Shennong's Classic of Materia Medica* as "fresh is especially good." Because they undergo no drying or complex processing, unstable substances in fresh Chinese medicinal herbs, such as volatile and heat-sensitive components, are not degraded or altered. The variety and content of chemical components are richer, and the natural proportions between components are not destroyed. Therefore, compared to processed products, the original medicinal properties and efficacy of Chinese medicinal herbs are fully preserved, resulting in more prominent therapeutic effects.

[0004] Traditional Chinese medicine (TCM) classifies pharyngitis under the category of "acute throat obstruction," believing it is mostly caused by wind-heat pathogens invading the throat and damaging the lungs, leading to swelling, obstruction, and pain in the throat; or by excessive pathogenic heat spreading from the lungs to the interior, burning the throat along the meridians, causing swelling and pain. Treatment principles focus on dispelling wind and clearing heat, promoting lung function and relieving sore throat, and detoxifying and moistening the throat. Existing medications are not very effective in treating pharyngitis. Western medicine mainly uses anti-inflammatory and antiviral drugs, which can only alleviate symptoms to a certain extent, with limited efficacy and a high risk of drug resistance and adverse reactions. TCM primarily uses lozenges and sprays, but these also mainly alleviate local symptoms, resulting in slow efficacy, large dosages, and long treatment times, with less than ideal results. Therefore, developing a drug formulation that is fast-acting, effective, less prone to recurrence, and highly safe is of significant practical importance. Summary of the Invention

[0005] (a) Technical problems to be solved

[0006] To address the shortcomings of existing technologies, this invention provides a traditional Chinese medicine composition for pharyngitis and its application.

[0007] (2) Technical solution

[0008] To achieve the above objectives, the present invention provides the following technical solution:

[0009] This invention provides a traditional Chinese medicine composition for pharyngitis, which is composed of the following raw materials in parts by weight: 10-30 parts of fresh mugwort leaves, 1-15 parts of fresh asparagus root, 3-20 parts of fresh mulberry leaves, and 1-15 parts of fresh licorice root.

[0010] Furthermore, the Chinese herbal medicine composition consists of the following raw materials in parts by weight: 15-25 parts of fresh mugwort leaves, 1-10 parts of fresh asparagus root, 5-15 parts of fresh mulberry leaves, and 1-10 parts of fresh licorice root.

[0011] Furthermore, the Chinese herbal medicine composition consists of the following raw materials in parts by weight: 20 parts fresh mugwort leaves, 5 parts fresh asparagus root, 10 parts fresh mulberry leaves, and 5 parts fresh licorice root.

[0012] Furthermore, in the aforementioned fresh herbal composition, the fresh mugwort leaves are the fresh leaves of *Artemisia argyi* Levl. et Vant. (Asteraceae); the fresh asparagus root is the fresh tuberous root of *Asparagus cochinchinensis* (Lour.) Merr. (Liliaceae); the fresh mulberry leaves are the fresh leaves of *Morus alba* L. (Moraceae); and the fresh licorice root and rhizome are the fresh roots and rhizomes of *Glycyrrhiza uralensis* Fisch., *Glycyrrhiza inflata* Bat., or *Glycyrrhiza glabra* L. (Fabaceae). All the above-mentioned medicinal materials are freshly harvested and have not undergone any processing or preparation before use.

[0013] This invention provides a method for preparing a fresh herbal composition for pharyngitis, comprising the following steps:

[0014] (1) Weigh out fresh mugwort leaves, fresh asparagus, fresh mulberry leaves and fresh licorice according to the above weight proportions, add an appropriate amount of pure water to the blender and juice them, then filter to obtain the filtrate.

[0015] (2) The filtrate is concentrated under vacuum to 0.5 g / mL.

[0016] The traditional Chinese medicine composition provided by this invention is used to prepare a drug for treating pharyngitis. The pharmacological effects of each raw material are as follows:

[0017] Fresh mugwort leaves: pungent and bitter in taste, cool in nature, and enter the liver, spleen, and kidney meridians; they have the effects of clearing heat and detoxifying, removing dampness and relieving itching. Fresh mugwort leaves can be used to treat hematemesis and epistaxis caused by blood heat, as well as itching caused by damp heat.

[0018] Fresh asparagus: sweet in taste, cold in nature, and enters the lung and kidney meridians; it has the effects of nourishing yin and moistening dryness, clearing the lungs and reducing fire, and is used for yin deficiency fever, coughing up blood, pulmonary atrophy, lung abscess, sore throat, diabetes, and constipation.

[0019] Fresh mulberry leaves: sweet and bitter in taste, cold in nature, and enter the lung and liver meridians; they have the effects of dispersing wind-heat, clearing the lungs and moistening dryness, and clearing the liver and improving eyesight. They are used for wind-heat colds, dry coughs due to lung heat, dizziness, headaches, and red and blurred vision.

[0020] Fresh licorice root: It has a sweet taste and cool properties. It enters the spleen, lung, heart, and stomach meridians. It has the effects of clearing heat and detoxifying, relieving phlegm and coughing. It is used for carbuncles, sores, sore throat, cough with excessive phlegm, etc.

[0021] (III) Beneficial Effects

[0022] This invention provides a traditional Chinese medicine (TCM) fresh herbal composition for pharyngitis and its application. The composition consists of four herbs: fresh mugwort leaves, fresh asparagus root, fresh mulberry leaves, and fresh licorice root. Through a precise combination of principal, assistant, and adjuvant herbs, it aims to clear heat and detoxify, moisten dryness and promote fluid production, reduce swelling and relieve pain, effectively treating pharyngitis and sore throat. The formula uses a large dose of fresh mugwort leaves as the principal herb, which has the effects of clearing heat and detoxifying, reducing swelling and relieving pain; fresh asparagus root is used as the assistant herb, which has the effects of moistening the lungs and promoting fluid production, and clearing deficiency heat; fresh mulberry leaves are used as the adjuvant herb, which has the effects of clearing the lungs and reducing fire, moistening the throat and eliminating phlegm, and can penetrate deep into the lung system, complementing the other herbs and significantly enhancing the clearing and moistening effects of the formula; fresh licorice root is used as the adjuvant herb, whose sweet nature can harmonize the other herbs, balance their properties, and simultaneously have the effects of moistening the throat and relieving cough, detoxifying, and relieving urgency, avoiding a greasy feeling. The entire formula achieves the effects of clearing heat and detoxifying, moistening dryness and promoting fluid production, and reducing swelling and relieving pain.

[0023] The fresh herbal composition of this invention can inhibit the secretion of inflammatory factors TNF-α and IL-6 by LPS-induced macrophages RAW264.7; it can significantly improve the food and water intake, scratching frequency, and pharyngeal mucosal congestion and swelling in rats with acute pharyngitis, reduce serum TNF-α, IL-1β, IL-6, IL-10, and PGE2 levels, and increase IFN-γ levels, effectively inhibiting the release of inflammatory factors, reducing inflammation levels, and improving pharyngeal tissue lesions. Moreover, its therapeutic effect is superior to that of its processed products and the positive drug Lanqin oral liquid. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0025] Example 1

[0026] A traditional Chinese medicine composition for pharyngitis, comprising the following raw materials in parts by weight: 20 parts fresh mugwort leaves, 5 parts fresh asparagus root, 10 parts fresh mulberry leaves, and 5 parts fresh licorice root.

[0027] The aforementioned fresh herbal composition comprises: fresh mugwort leaves (Artemisia argyi Levl. et Vant., Asteraceae); fresh asparagus root (Asparagus cochinchinensis (Lour.) Merr., Liliaceae); fresh mulberry leaves (Morus alba L., Moraceae); and fresh licorice root and rhizome (Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., or Glycyrrhiza glabra L., Fabaceae). All the above-mentioned medicinal materials are freshly harvested and have not undergone any processing or preparation before use.

[0028] The preparation method of the fresh Chinese herbal medicine composition includes the following steps:

[0029] (1) Weigh out fresh mugwort leaves, fresh asparagus, fresh mulberry leaves and fresh licorice according to the above weight proportions, add an appropriate amount of pure water to the blender and juice them, then filter to obtain the filtrate.

[0030] (2) The filtrate is concentrated by freezing and vacuum.

[0031] Example 2

[0032] The difference between this embodiment and Embodiment 1 is that the fresh Chinese herbal medicine composition includes the following raw materials in parts by weight: 10 parts fresh mugwort leaves, 1 part fresh asparagus root, 3 parts fresh mulberry leaves, and 1 part fresh licorice root.

[0033] Example 3

[0034] The difference between this embodiment and Embodiment 1 is that the fresh Chinese herbal medicine composition includes the following raw materials in parts by weight: 30 parts of fresh mugwort leaves, 15 parts of fresh asparagus root, 20 parts of fresh mulberry leaves, and 15 parts of fresh licorice root.

[0035] Example 4

[0036] The difference between this embodiment and Embodiment 1 is that the fresh Chinese herbal medicine composition includes the following raw materials in parts by weight: 12 parts fresh mugwort leaves, 3 parts fresh asparagus root, 5 parts fresh mulberry leaves, and 3 parts fresh licorice root.

[0037] Example 5

[0038] The difference between this embodiment and Embodiment 1 is that the fresh Chinese herbal medicine composition includes the following raw materials in parts by weight: 15 parts fresh mugwort leaves, 4 parts fresh asparagus, 7 parts fresh mulberry leaves, and 3 parts fresh licorice.

[0039] Example 6

[0040] The difference between this embodiment and Embodiment 1 is that the fresh Chinese herbal medicine composition includes the following raw materials in parts by weight: 24 parts fresh mugwort leaves, 7 parts fresh asparagus, 12 parts fresh mulberry leaves, and 7 parts fresh licorice.

[0041] Example 7

[0042] The difference between this embodiment and Embodiment 1 is that the fresh Chinese herbal medicine composition includes the following raw materials in parts by weight: 28 parts of fresh mugwort leaves, 10 parts of fresh asparagus root, 15 parts of fresh mulberry leaves, and 12 parts of fresh licorice root.

[0043] Test Example 1

[0044] 1. Materials

[0045] Experiment 1: 20 parts fresh mugwort leaves, 5 parts fresh asparagus, 10 parts fresh mulberry leaves, and 5 parts fresh licorice.

[0046] Experiment 2: 20 portions of fresh Panax notoginseng, 5 portions of fresh Asparagus cochinchinensis, 10 portions of fresh mulberry leaves, and 5 portions of fresh licorice.

[0047] Experimental Group 3: 20 portions of fresh mugwort leaves and 5 portions of fresh licorice root.

[0048] Experimental Group 4: 5 parts fresh asparagus, 10 parts fresh mulberry leaves, and 5 parts fresh licorice.

[0049] The fresh herbal combinations described herein include: fresh Artemisia argyi Levl. et Vant. (Asteraceae); fresh Asparagus cochinchinensis (Lour.) Merr. (Liliaceae); fresh Mulberry leaves (Morus alba L. (Moraceae); fresh Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., or Glycyrrhiza glabra L. (Fabaceae); and fresh Panax notoginseng (Burk.) FHChen (Araliaceae). All the above-mentioned medicinal materials are freshly harvested and have not undergone any processing or preparation before use.

[0050] Weigh each raw material according to the above-mentioned weight proportions, add an appropriate amount of pure water to juice using a high-speed blender, and filter to obtain filtrate; freeze and vacuum concentrate the filtrate to 0.5g / mL to obtain the Chinese herbal medicine stock solution for test groups 1-4, and store it in a refrigerator at 0-4℃ for later use.

[0051] Dexamethasone, Zhejiang Xianju Pharmaceutical Co., Ltd., product batch number LB2146.

[0052] Lanqin Oral Solution, Yangtze River Pharmaceutical Group Co., Ltd., product batch number 21020682.

[0053] 2 Methods

[0054] 2.1 Cell Culture

[0055] RAW264.7 cells were revived in DMEM complete medium (containing 10% fetal bovine serum and 1% anti-penicillin-streptomycin mixture) and cultured in a 37°C, 5% CO2 environment. The medium was changed every 2-3 days and the cells were passaged at a ratio of 1:3.

[0056] 2.2 Cell viability assay

[0057] RAW264.7 cells in logarithmic growth phase were used at a concentration of 2 × 10⁻⁶. 5 Cells were seeded at a concentration of [number] cells / well in 96-well plates and cultured for 24 h. A blank control group and experimental groups 1-4 were included, with three replicates per group. The control group received 200 μL of culture medium, while experimental groups 1-4 received 200 μL of drug-containing culture medium. After 24 h of culture, the culture medium was changed, adding 180 μL of culture medium and 20 μL of CCK-8 to each well. After incubation for 1-4 h, the absorbance was measured at 450 nm using a microplate reader, and cell viability was calculated. Cell viability = (OD [value missing]) / [value missing] 药物组 -OD 空白组 ) / OD空白组 ×100%.

[0058] 2.3 Detection of cellular inflammatory factors

[0059] RAW264.7 cells in logarithmic growth phase were used at a concentration of 2 × 10⁻⁶. 5 Inoculate the culture medium at a concentration of [number] cells / well in 96-well plates and incubate for 24 h. A blank control group and experimental groups 1-4 were included, with three replicates per group. Culture medium containing experimental groups 1-4, dexamethasone, and Lanqin oral liquid were added to each well, while the blank control group received an equal volume of culture medium. One h later, LPS was added to each well for stimulation at a final concentration of 1 μg / mL. After 24 h of incubation, the culture medium was collected and cultured at 3000 rpm. -1 Under the specified conditions, the cell culture medium of each group was continuously centrifuged for 15 minutes, and the supernatant was collected. The secretion of TNF-α and IL-6 in the supernatant was detected by ELISA.

[0060] 2.4 Statistical Methods

[0061] Data analysis was performed using SPSS v.21.0 statistical software. Results are expressed as mean values. ± standard deviation (s) is used for two independent samples using one-way ANOVA.

[0062] 3 Experimental Results

[0063] 3.1 Effects of each drug group on cell viability

[0064] The effects of each drug group on the viability of RAW264.7 cells are shown in Table 1. Compared with the blank control group, there was no significant difference in cell viability among the drug groups (P>0.05), and all were above 95%, indicating that each drug group had good safety.

[0065] Table 1. Effects of each drug group on the viability of RAW264.7 cells.

[0066] Grouping Cell vitality Blank control group 100.26±0.67 Experiment 1 group 98.76±0.31 Experimental Group 2 96.42±3.69 Experiment 3 groups 97.40±2.90 Experiment 4 groups 96.34±4.30

[0067] 3.2 Effects of each drug group on the release of inflammatory factors from RAW264.7 cells

[0068] The effects of each drug group on LPS-induced secretion of TNF-α and IL-6 in RAW264.7 cells are shown in Table 2. Compared with the blank control group, the secretion levels of TNF-α and IL-6 in the model group RAW264.7 cells were significantly increased (P<0.05), indicating that the inflammation model was successfully constructed. Compared with the model control group, each drug group could inhibit the secretion of TNF-α and IL-6 in RAW264.7 cells to varying degrees. Among them, the secretion levels of TNF-α and IL-6 in experimental group 1 were the lowest, showing the most significant anti-inflammatory effect, which was superior to the traditional Chinese medicine positive drug Lanqin oral liquid group, and the difference was statistically significant (P<0.05). Experimental groups 2, 3, and 4 also showed certain anti-inflammatory effects. However, the anti-inflammatory effect of experimental group 2 was not as significant as that of experimental group 1 because fresh Panax notoginseng was used instead of fresh Artemisia argyi. The anti-inflammatory effect of experimental group 3 was slightly inferior to that of experimental group 1 because it lacked the assistant and adjuvant drugs. The anti-inflammatory effect of experimental group 4 was relatively weak because it lacked the principal drug. The above results indicate that the components in the fresh herbal composition of this invention have a synergistic effect, and none of them can be omitted. Furthermore, fresh mugwort leaves play an irreplaceable role in this formula.

[0069] Table 2. Effects of each drug group on the secretion of TNF-α and IL-6 by RAW264.7 cells.

[0070]

[0071] Note: Different letters in the superscript of data in the same column indicate significant differences (P<0.05), while the same letter indicates no significant differences (P>0.05).

[0072] Experimental Example 2

[0073] 1. Experimental Materials

[0074] 6-8 week old SD rats, weighing 200-220g, half male and half female, were provided by Jinan Pengyue Experimental Animal Breeding Co., Ltd.

[0075] Artemisia argyi, Asparagus cochinchinensis, mulberry leaves, and licorice were all purchased from Zhang Zhongjing Pharmacy.

[0076] 2 Experimental Methods

[0077] 2.1 Preparation of experimental drugs

[0078] Experiment 1: 20 parts fresh mugwort leaves, 5 parts fresh asparagus, 10 parts fresh mulberry leaves, and 5 parts fresh licorice.

[0079] Weigh each raw material according to the above weight proportions, add an appropriate amount of pure water to the blender to extract the juice, filter to obtain the filtrate; freeze and vacuum concentrate the filtrate to 0.5g / mL to obtain the original Chinese medicine liquid of test group 1.

[0080] Experimental Group 2: 20 parts of Artemisia argyi, 5 parts of Asparagus cochinchinensis, 10 parts of Mulberry leaves, and 5 parts of Glycyrrhiza uralensis.

[0081] Weigh each raw material according to the above weight proportions, wash and chop them, mix them, add water to extract, filter, and freeze and vacuum concentrate to 0.5g / mL to obtain the original Chinese medicine solution of the two test groups.

[0082] The aforementioned fresh herbal composition comprises: fresh mugwort leaves (Artemisia argyi Levl. et Vant., Asteraceae); fresh asparagus root (Asparagus cochinchinensis (Lour.) Merr., Liliaceae); fresh mulberry leaves (Morus alba L., Moraceae); and fresh licorice root and rhizome (Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat., or Glycyrrhiza glabra L., Fabaceae). All the above-mentioned medicinal materials are freshly harvested and have not undergone any processing or preparation before use.

[0083] 2.2 Establishment of an acute pharyngitis model

[0084] Sixty SD rats were acclimatized for one week and then randomly divided into four groups: a blank control group, a model control group, a dexamethasone group, a Lanqin oral liquid group, and experimental groups 1-2, with 10 rats in each group. 20% ammonia water was sprayed into the pharynx of the rats using a laryngeal sprayer, 3 sprays per application (0.2-0.4 mL), twice a day for 3 consecutive days. The blank control group was sprayed with the same amount of pure water using the same method. During the modeling period, the rats had free access to food and water. The rats' appearance was observed daily, including their mental state, fur, scratching frequency, food and water intake, and redness and swelling of the pharyngeal mucosa. Successful modeling was indicated by increased pharyngeal secretions, congestion and redness of the mucosa (bright red in color), frequent scratching of the sides or lower part of the mouth, ulceration and bleeding in severe cases, coughing and wheezing, increased activity, and increased water intake.

[0085] 2.3 Grouping and Dosing

[0086] Rats that successfully developed the model were administered the drug via gavage at a dose of 1 mL / 100g, daily at 10:00 AM. The blank control group and the model control group were given the same volume of pure water. The administration was once a day for 7 consecutive days. The bedding of each group of rats was changed regularly, and the cages were cleaned promptly.

[0087] 2.4 Observation of Apparent Indicators

[0088] During the modeling and drug administration periods, the mental state, body weight, food and water intake, fur luster, and scratching frequency of rats in each group were observed. The morphology of the pharyngeal mucosa, the area of ​​congestion and swelling, secretions, and coughing and wheezing were also observed. Semi-quantitative scores were calculated based on the apparent indicators according to Table 3.

[0089] Table 3 Semi-quantitative integral table of apparent indicators

[0090]

[0091] 2.5 Serum marker detection

[0092] Rats were fasted for 12 hours before blood collection. Two hours after the last administration, rats were weighed and anesthetized with 45 mg / kg of 2% sodium pentobarbital. Blood was collected from the abdominal aorta and placed in a refrigerator at 2-8℃ for 1-2 hours. The blood was then centrifuged at 3500 rpm for 15 minutes to separate the serum. The levels of TNF-α, IL-1β, IL-6, IL-10, PGE2, and IFN-γ in the rat serum were detected by ELISA.

[0093] 2.6 Histopathological observation of pharyngeal tissue

[0094] Rats were fasted for 12 hours before tissue collection. Two hours after the last administration of the drug, rats were weighed and anesthetized with 45 mg / kg body weight of 2% sodium pentobarbital. Pharyngeal tissue was collected, surrounding tissues and muscles were dissected, and the tissues were rinsed with physiological saline. The tissues were then fixed in 4% paraformaldehyde tissue fixative. After 24 hours, the tissues were dehydrated, cleared, embedded in paraffin, sectioned, stained with H&E, and the pathological morphological changes of the rat pharyngeal tissue were observed under a microscope. A scoring table was derived based on the pathological differences among the groups, as shown in Table 4.

[0095] Table 4. Pharyngeal Tissue Pathology Scoring Table

[0096]

[0097] 2.7 Statistical Analysis

[0098] Data analysis was performed using SPSS v.21.0 statistical software. Results are expressed as mean values. Expressed as ± standard deviation (s), two independent samples were analyzed using one-way ANOVA. Pathology scores were analyzed using the Ridit test.

[0099] 3 Results

[0100] 3.1 Apparent Indicators

[0101] Table 5 shows the phenotypic scores of each group of rats with acute pharyngitis. In the control group, rats had normal hair, food and water intake, no pharyngeal redness or swelling, and no scratching of the pharynx. Compared with the control group, rats in the model group had significantly red and swollen pharynx, a larger area of ​​swelling, abundant secretions, rapid coughing and wheezing, disheveled and dull hair, frequent scratching of the oropharynx, and significantly reduced food intake. Compared with the model group, rats in each treatment group showed varying degrees of improvement in food and water intake, body weight, mental state, and pharyngeal mucosal redness and swelling. In Groups 1 and 2, water intake gradually recovered, food intake gradually increased, pharyngeal mucosal congestion and swelling gradually disappeared, the area of ​​swelling decreased, and scratching of the pharynx and lips decreased (P<0.01). However, the phenotypic score of mice in Group 1 was lower than that of the Lanqin oral liquid group and Group 2, indicating that Group 1 had a better therapeutic effect on acute pharyngitis than the Lanqin oral liquid group and Group 2.

[0102] Table 5. Phenotypic scores of rats in the acute pharyngitis model group for each group.

[0103]

[0104]

[0105] Note: Compared with the blank control group ## P<0.01; compared with the model control group, ** P<0.01.

[0106] 3.2 Effects of each group of drugs on serum inflammatory factors in rats

[0107] The effects of each drug group on serum inflammatory factor levels in rats with acute pharyngitis are shown in Tables 6 and 7. Compared with the blank control group, the serum levels of TNF-α, IL-1β, IL-6, IL-10, and PGE2 in the model group rats were significantly increased, while the IFN-γ level was significantly decreased (P<0.01), indicating that the ammonia-induced rat model of acute pharyngitis was successfully established. Compared with the model group, the serum levels of TNF-α, IL-1β, IL-6, IL-10, and PGE2 in each drug-treated group were significantly decreased (P<0.01), while the IFN-γ level was significantly increased (P<0.01). Furthermore, the effect of experimental group 1 was superior to that of the traditional Chinese medicine positive control drug Lanqin oral liquid group and experimental group 2.

[0108] Table 6. Effects of each drug group on serum TNF-α, IL-1β, and IL-6 levels in rats.

[0109]

[0110] Note: Compared with the blank control group # P<0.05, ## P<0.01; compared with the model control group, *P<0.05, ** P<0.01.

[0111] Table 7. Effects of each drug group on serum IL-10, PGE2, and IFN-γ in rats.

[0112]

[0113]

[0114] Note: Compared with the blank control group # P<0.05, ## P<0.01; compared with the model control group, * P<0.05, ** P<0.01.

[0115] 3.3 Histopathological changes in the pharynx

[0116] The histopathological results of the pharyngeal tissue in each group are shown in Table 8. In the blank group, the mucosal epithelium of the rats was smooth and flat, and there was no dilation of the submucosal glands or inflammatory cell infiltration. Compared with the blank group, the pharyngeal tissue of the model group rats showed significant pathological changes, with damage and shedding of the mucosal epithelium, hyperplasia and thickening, significant congestion and dilation of blood vessels in the submucosal layer, a large number of inflammatory cells infiltrating, vacuolation of glands, and obvious swelling (P<0.01). Compared with the model group, all treatment groups significantly improved the pharyngeal tissue lesions, reduced mucosal epithelial damage, mild dilation of submucosal glands, significantly reduced vascular congestion and dilation, and significantly reduced inflammatory cell infiltration (P<0.01). Moreover, the treatment effect of experimental group 1 was better than that of the traditional Chinese medicine positive control drug Lanqin oral liquid group and experimental group 2.

[0117] Table 8. Effects of each group on the pathological morphology of pharyngeal tissue in rats with acute pharyngitis.

[0118] Grouping - + ++ +++ P Blank control group 10 0 0 0 Model control group 0 1 2 7 <![CDATA[ ## ]]> Dexamethasone group 1 5 4 0 <![CDATA[ ** <!-- 8 -->]]> Lanqin Oral Liquid Group 1 4 4 1 <![CDATA[ ** ]]> Experiment 1 group 1 5 4 0 <![CDATA[ ** ]]> Experimental Group 2 0 4 4 2 <![CDATA[ ** ]]>

[0119] Note: Compared with the blank control group ## P<0.01; compared with the model control group, ** P<0.01.

[0120] In summary, this invention adheres to the principles of traditional Chinese medicine theory and treatment based on syndrome differentiation. It utilizes the rich active ingredients, rapid efficacy, and high quality of fresh herbs to select the fresh herbal composition of this invention. The experimental results further demonstrate that the fresh herbal composition of this invention can significantly improve water intake, scratching frequency, and pharyngeal mucosal congestion and swelling in rats with acute pharyngitis, reduce serum TNF-α, IL-1β, IL-6, IL-10, and PGE2 levels, increase IFN-γ levels, effectively inhibit the release of inflammatory factors, reduce inflammation levels, and improve pharyngeal tissue lesions. Furthermore, its therapeutic effect is superior to that of the traditional Chinese medicine positive control drug, *Lanting* oral liquid. This invention provides a new approach and treatment method for pharyngitis.

[0121] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A traditional Chinese medicine composition for treating pharyngitis, characterized in that, The Chinese herbal medicine composition consists of the following raw materials in parts by weight: 20 parts fresh mugwort leaves, 5 parts fresh asparagus root, 10 parts fresh mulberry leaves, and 5 parts fresh licorice root.

2. The traditional Chinese medicine composition for treating pharyngitis as described in claim 1, characterized in that, In the aforementioned fresh herbal composition, the fresh mugwort leaves are from the plant Artemisia argyi (Artemisia argyi), belonging to the Asteraceae family. Artemisia argyi Levl.et Vant. Fresh leaves; fresh asparagus is the asparagus plant of the lily family. Asparagus cochinchinensis Fresh tuberous roots of (Lour.) Merr.; fresh mulberry leaves are from the mulberry tree (Morus alba) of the Moraceae family. Morus alba Fresh leaves of L.; fresh licorice is the licorice plant of the legume family. Glycyrrhiza uralensis Fisch. or licorice with inflated fruit Glycyrrhiza inflata Bat. or Licorice root Glycyrrhiza glabra Fresh roots and rhizomes of L.; fresh herbs are harvested fresh and used without any processing or preparation.

3. The method for preparing a traditional Chinese medicine fresh herb composition for treating pharyngitis as described in claim 1, characterized in that, Includes the following steps: Weigh out fresh mugwort leaves, fresh asparagus root, fresh mulberry leaves, and fresh licorice root according to the above weight proportions. Use a high-speed blender with an appropriate amount of pure water to extract the juice, filter it to obtain the filtrate, and then freeze and vacuum concentrate the filtrate to obtain the final product.

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