Application method of micro-fluidic chip

Abstract

The invention discloses a use method of a micro-fluidic chip, the micro-fluidic chip is provided with a sample flow channel, a reagent flow channel, a detection flow channel, a common flow channel and a mixing cavity, and the common flow channel can be communicated with the sample flow channel, the reagent flow channel and the detection flow channel. The uniform mixing cavity is located on the path of the detection flow channel and provided with an outlet and an inlet, the uniform mixing cavity comprises a uniform mixing bend and a uniform mixing chamber, and the uniform mixing bend is a bent flow channel and located on the path of the detection flow channel. The width size of the uniform mixing chamber is greater than the diameter size of the detection flow channel; the use method of the micro-fluidic chip comprises the following steps: S11, driving a sample in the sample flow channel to enter the public flow channel; s12, driving the reagent in the reagent runner to enter the public runner; and S13, driving the sample and the reagent in the public flow channel to enter the inlet of the uniform mixing cavity from the detection flow channel, entering the uniform mixing curve from the inlet of the uniform mixing cavity and then flowing into the uniform mixing chamber to form uniformly mixed liquid, and then driving the uniformly mixed liquid to flow into the detection flow channel from the outlet of the uniform mixing cavity again and flow out from the detection flow channel.

Description

Technical Field

[0001] The present invention relates to the technical field of microfluidic chips, and in particular to a method for using a microfluidic chip. Background Art

[0002] As human life expectancy increases, the importance of health care and maintenance has received more and more attention. Urine can reflect a person's health status to a certain extent. Quantitative analysis of urine components can help us understand diseases such as the pancreas and kidneys. Urinalysis is an important indicator for detecting a person's physical condition. It can usually detect pH, protein, occult blood, specific gravity, glucose, ketone bodies, urobilinogen, nitrates, white blood cells, bilirubin and vitamin C, reflecting a series of indicators of the body. Urine tests are usually conducted in hospitals. Due to the shortage of medical resources, the examinees often have to queue up to register, see a doctor, pay fees, collect urine, wait for batches of urine tests, and then queue up to get the results, which is time-consuming and laborious.

[0003] At present, there are urine testers for home use on the market. When the reagents flow in the flow channel plate of the urine tester, they cannot be fully mixed and bubbles are generated, causing unstable test results. Summary of the invention

[0004] The purpose of the present invention is to provide a method for using a microfluidic chip to solve the problems of the prior art.

[0005] In order to solve the above technical problems, an embodiment of the present invention provides a method for using a microfluidic chip, wherein the microfluidic chip is provided with: Sample flow channel; Reagent flow channel; Detect flow channel; a common flow channel, the common flow channel being operably connected to the sample flow channel, the reagent flow channel and the detection flow channel; A mixing chamber, the mixing chamber is located on the path of the detection flow channel and has an outlet and an inlet, and the mixing chamber includes: a mixing bend, which is a curved flow channel and is located on the path of the detection flow channel; and At least one mixing chamber, wherein the width of the mixing chamber is greater than the diameter of the detection channel; The method for using the microfluidic chip comprises the steps of: S11, driving the sample in the sample flow channel into the common flow channel; S12, driving the reagent in the reagent flow channel into the common flow channel; S13, drive the sample and reagent in the common flow channel to enter the inlet of the mixing chamber from the detection flow channel, enter the mixing bend from the inlet of the mixing chamber, and then flow into the mixing chamber to form a mixed liquid, and then drive the mixed liquid to flow into the detection flow channel again from the outlet of the mixing chamber, and flow out of the detection flow channel.

[0006] In one embodiment, the mixing chamber is located above the mixing bend, and the inlet of the mixing chamber is below the outlet of the mixing chamber; In step S13, the sample and reagent in the common flow channel are driven to enter the entrance of the mixing chamber from the detection flow channel, enter the mixing bend from the entrance of the mixing chamber, and then flow into the mixing chamber. Then the mixed liquid flows back from the mixing chamber into the mixing bend, and then the mixed liquid is driven to flow from the mixing bend into the mixing chamber. Finally, the mixed liquid is driven to enter the detection flow channel from the mixing chamber again and flow out from the detection flow channel.

[0007] In one embodiment, the mixing chamber is located above the mixing bend; In step S13, the sample and reagent in the common flow channel are driven to enter the entrance of the mixing chamber from the detection flow channel, enter the mixing curve from the entrance of the mixing chamber, and then flow into the mixing chamber. Then, the mixed liquid flows back from the mixing chamber into the mixing curve. After staying for a preset period of time, the mixed liquid is driven to flow from the mixing curve into the mixing chamber. Finally, the mixed liquid is driven from the mixing chamber to enter the detection flow channel again and flow out of the detection flow channel.

[0008] In one embodiment, the mixing chamber includes a plurality of mixing bends and a plurality of mixing chambers, and the plurality of mixing bends and the plurality of mixing chambers are arranged at intervals and communicated with each other; In step S13, the sample and reagent in the common flow channel are driven to enter the inlet of the mixing chamber from the detection flow channel, enter the multiple mixing bends and multiple mixing chambers from the inlet of the mixing chamber, and finally drive the mixed liquid to flow out of the detection flow channel.

[0009] In one embodiment, each of the mixing chambers has an inlet and an outlet, respectively, the inlet and outlet of each mixing chamber are respectively connected to the adjacent mixing bend, and the inlet of each mixing chamber is located above the outlet; In step S13, after the sample and reagent enter the mixing chamber, they flow from one of the mixing bends through an adjacent one of the inlets into the mixing chamber, and then flow from the outlet into another mixing bend. The sample and reagent flow continuously in multiple mixing chambers and multiple mixing bends to form the mixed liquid.

[0010] In one embodiment, the mixing channel is an S-shaped channel and includes at least two horizontal channels and at least one turning channel, and any one of the turning channels is located at both ends of the two horizontal channels; The horizontal flow channel extends in a horizontal direction, and the turning flow channel includes a curved inner arc and an outer arc. Both the inner arc and the outer arc are arc-shaped, and the inner diameter of the inner arc is smaller than the inner diameter of the outer arc.

[0011] In one embodiment, the inner wall of the mixing chamber includes a first curved surface and a second curved surface opposite to the first curved surface, and the first curved surface and the second curved surface are curved surfaces protruding toward the outside.

[0012] In one embodiment, each of the mixing chambers is connected to the horizontal flow channels of the two mixing bends, and the two horizontal flow channels are radially symmetrically arranged at the top and bottom of the mixing chamber; In step S13, the sample and reagent in the common flow channel are driven to enter the entrance of the mixing chamber from the detection flow channel, enter one of the mixing bends from the entrance of the mixing chamber, and then flow into the mixing chamber, and then flow from the mixing chamber into another mixing bend to form a mixed liquid. After staying for a preset period of time, the mixed liquid flows back into the mixing chamber, and the mixed liquid is driven to be discharged from the mixing bend.

[0013] In one embodiment, the microfluidic chip is further provided with a clean water flow channel; The common flow channel is operably connected to the clean water flow channel; After step S13, step S14 is also included: driving the water in the clean water channel into the common channel, and then driving the water in the common channel into the detection channel, and then entering the mixing chamber from the inlet of the mixing chamber, and then entering the detection channel again from the outlet of the mixing chamber, and being discharged from the detection channel.

[0014] In one embodiment, the microfluidic chip includes a flow channel plate and a cover plate covering the front side of the flow channel plate; The sample flow channel, the reagent flow channel, the detection flow channel, the common flow channel and the mixing chamber are formed by the depression of the front side surface of the flow channel plate; The flow channel plate is provided with: A central hole, the central hole passes through the flow channel plate and is connected to the inner end of the common flow channel; A plurality of circumferential holes, wherein the plurality of circumferential holes are arranged along a circumference formed with the central hole as the center; The inner end of the common flow channel is in communication with the central hole, and the outer end of the common flow channel is connected to a pump via a driving pipeline; The inner end of the sample flow channel is in communication with one of the circumferential holes; The inner end of the reagent flow channel is in communication with one of the circumferential holes; The inner end of the detection flow channel is connected to one of the circumferential holes; The central hole is connected to a transfer channel of a rotor, and the outer end of the transfer channel can be connected to any one of the circumferential holes as the rotor rotates; Step S11, the rotor rotates, the transfer channel is connected to the sample flow channel through one of the circumferential holes, the pump draws out the air in the common flow channel and the sample flow channel, and draws the sample in the sample flow channel into the common flow channel; Step S12, the rotor rotates, the transfer channel is connected to the reagent flow channel through one of the circumferential holes, the pump extracts the air in the common flow channel and the reagent flow channel, and draws the sample in the reagent flow channel into the common flow channel; In step S13, the rotor rotates, and the transfer channel is connected to the detection channel through one of the circumferential holes; the pump drives the sample and reagent in the common channel to enter the inlet of the mixing chamber from the detection channel, and then enter the mixing bend from the inlet of the mixing chamber and flow into the mixing chamber to form a mixed liquid, and then drives the mixed liquid to flow into the detection channel again from the outlet of the mixing chamber and flow out of the detection channel.

[0015] In one embodiment, the flow channel plate is provided with two sample flow channels, namely an upper sample flow channel and a lower sample flow channel; The inner end of the lower sample flow channel and the inner end of the upper sample flow channel are respectively connected to the two circumferential holes; The lower sample flow is connected to the collection chamber containing the sample through the lower sample pipe, and a burette is provided on the lower sample pipe; The upper sample flow channel is connected to the burette through an upper sample pipe; In step S11, the rotor rotates to align the outer end of the transfer channel with the circumferential hole connected to the upper sample flow channel, and the pump drives the driving pipeline to extract the air in the common flow channel, the transfer channel, the upper sample flow channel and the burette; Then the rotor rotates, and aligns the outer end of the transfer channel with the circumferential hole connected to the lower sample flow channel, and draws the sample in the collection chamber from the lower sample flow channel to the common flow channel.

[0016] In one embodiment, the flow channel plate is further provided with a clean water flow channel, and the clean water flow channel is connected to one of the circumferential holes; After step S13, step S14 is also included: the rotor rotates, the transfer channel is connected with the clean water flow channel through one of the circumferential holes, the pump drives the water in the clean water flow channel to enter the common flow channel, and then drives the water in the common flow channel to enter the detection flow channel, and then enters the mixing chamber from the inlet of the mixing chamber, and then enters the detection flow channel again from the outlet of the mixing chamber, and is discharged from the detection flow channel.

[0017] In one embodiment, the flow channel plate is provided with an upper clean water flow channel and a lower clean water flow channel, and the inner end of the upper clean water flow channel and the inner end of the lower clean water flow channel are respectively connected to the two circumferential holes; The lower clean water flows through a lower clean water pipe and is connected to a water collection chamber, and a burette is provided on the lower clean water pipe; The upper clear water flow channel is connected to the burette through an upper clear water pipeline; After step S13, step S14 is also included: the rotor rotates to align the outer end of the transfer channel with the circumferential hole connected to the upper clear water flow channel, and the pump pumps the air in the common flow channel, the transfer channel, the upper clear water flow channel and the burette to the outside; Then the rotor rotates to align the outer end of the transfer channel with the circumferential hole connected to the lower clean water flow channel, and the clean water in the collection chamber is pumped from the lower clean water flow channel to the common flow channel. The rotor rotates to align the common flow channel with the circumferential hole of the detection flow channel, and the water in the common flow channel is passed through the detection flow channel in turn and discharged from the detection flow channel.

[0018] In one embodiment, the flow channel plate is further provided with an air flow channel, the inner end of the air flow channel is connected to another of the circumferential holes; Step S13, the rotor rotates, and the transfer channel is connected to the detection channel through one of the circumferential holes; the rotor rotates so that the transfer channel is aligned with the circumferential hole connected to the air, so that the common channel is connected to the air channel, and the pump draws air from the air channel into the common channel; Then the rotor rotates, and the transfer channel is connected to the detection flow channel through one of the circumferential holes; the pump drives the sample and reagent in the common flow channel to enter the inlet of the mixing chamber from the detection flow channel, enter the mixing bend from the inlet of the mixing chamber, and then flow into the mixing chamber to form a mixed liquid, and then drives the mixed liquid to flow into the detection flow channel again from the outlet of the mixing chamber, and then flow out of the detection flow channel.

[0019] The microfluidic chip of the present invention is provided with a mixing chamber, which can reduce bubbles in the liquid, ensure the mixing effect of the sample and the reagent, and increase the accuracy of the test. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a stereoscopic diagram of a urine testing instrument according to an embodiment of the present invention.

[0021] Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 8 They are Figure 1 An exploded view of the urine testing instrument of the illustrated embodiment.

[0022] Fig. 9 yes Figure 1-Figure 8 A perspective view of the locking element in the illustrated embodiment.

[0023] Fig.10 and Fig.11 They are Figure 2 A perspective view of the kit in the illustrated embodiment.

[0024] Fig.12 and Fig.13 They are Figure 2 A perspective view of the cover plate in the illustrated embodiment.

[0025] Fig.14 yes Figure 2 A three-dimensional view of the flow channel plate in the illustrated embodiment.

[0026] Fig.15 yes Fig.14 A partial enlarged view of area A of the flow channel plate.

[0027] Fig.16 yes Fig.14 A partial enlarged view of area B of the flow channel plate.

[0028] Fig.17 yes Figure 2 A three-dimensional view of the flow channel plate in the illustrated embodiment.

[0029] Fig.18 yes Figure 4 and Figure 5 A perspective view of the first adapter of the illustrated embodiment.

[0030] Fig.19 yes Figure 4 and Figure 5 An assembly diagram of the reagent box, the flow channel plate, the cover plate, the first adapter, the rotor and the protective cover in the illustrated embodiment.

[0031] Fig. 20 yes Figure 1 An exploded view of the first housing in the illustrated embodiment.

[0032] Fig.21 yes Figure 2 A three-dimensional view of the detection plate in the illustrated embodiment.

[0033] Fig. 22 and Fig.23 yes Figure 2 Exploded view of the protective cover, rotor, compression spring and convex ring in the illustrated embodiment.

[0034] Fig.24 yes Figure 1 Exploded view of the second housing, peristaltic pump, plunger pump and motor in the illustrated embodiment.

[0035] Fig.25 It is a three-dimensional diagram of a collector according to an embodiment of the present invention.

[0036] Fig.26 and Fig. 27 They are Fig.25 An exploded view of the collector of the illustrated embodiment.

[0037] Fig.28 It is a stereoscopic diagram of a collector according to another embodiment of the present invention.

[0038] Fig.29 and Fig.30 They are Fig.28 An exploded view of the collector of the illustrated embodiment.

[0039] Fig.31 It is a flow chart of a control method of a urine testing instrument according to an embodiment of the present invention.

[0040] Fig.32 It is a fluid circuit diagram of a urine testing instrument according to an embodiment of the present invention. DETAILED DESCRIPTION

[0041] In order to make the purpose, technical scheme and advantages of the present invention clearer, the following will be described in detail with reference to the accompanying drawings. However, it will be appreciated by those skilled in the art that in the various embodiments of the present invention, many technical details are provided in order to enable the reader to better understand the present application. However, even without these technical details and various changes and modifications based on the following embodiments, the technical scheme claimed for protection in the claims of the present application can be implemented.

[0042] Unless the context requires otherwise, throughout the specification and claims, the word "comprise" and variations such as "include" and "have" should be construed in an open, inclusive sense, ie, should be interpreted as "including, but not limited to."

[0043] The following will be described in detail with reference to the accompanying drawings to provide a clearer understanding of the purpose, features and advantages of the present invention. It should be understood that the embodiments shown in the accompanying drawings are not intended to limit the scope of the present invention, but are only intended to illustrate the essential spirit of the technical solution of the present invention.

[0044] References throughout the specification to "one embodiment" or "an embodiment" indicate that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of "in one embodiment" or "in an embodiment" in various places throughout the specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any manner in one or more embodiments.

[0045] As used in this specification and the appended claims, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise. It should be noted that the term "or" is generally employed in its sense including "and / or" unless the context clearly dictates otherwise.

[0046] In the following description, in order to clearly show the structure and working mode of the present invention, many directional words will be used for description, but the words "front", "back", "left", "right", "outside", "inside", "outward", "inward", "up", "down", etc. should be understood as convenient terms and should not be understood as restrictive terms.

[0047] The present invention relates to a urine testing instrument 100, such as Figure 1-4 As shown, the urine tester 100 includes a housing 1, a collector 4, a reagent kit 2 located in the housing 1, a rotary cutting valve, a pump and a detection mechanism, wherein the rotary cutting valve includes a microfluidic chip, a rotor 6 and a driving member 93, wherein the reagent kit 2 is used to hold a variety of reagents, and the collector 4 is provided with a collection chamber, which includes a sample collection chamber 411 and a clean water collection chamber 412, wherein the sample collection chamber 411 is used to collect samples, and the clean water collection chamber 412 is used to store water. The flow channel plate 3 is used to provide multiple flow channels for the confluence of reagents and samples. Figure 5 In the embodiment, the detection mechanism includes a detector and a detection plate 5, wherein the detection plate 5 is provided with a detection cavity, which is used to receive samples and reagents from the flow channel plate 3, and the detection cavity is used to detect the samples in the detection cavity. The rotor 6 is used to cooperate with the flow channel plate 3 to facilitate the collection of samples and reagents, and to push the samples and reagents into the detection mechanism for detection. Figure 22-Figure 24 The driving member 93 is connected to the rotor 6 and is used to drive the rotor 6 to rotate.

[0048] Specifically, if Figure 1As shown, the housing 1 includes a first shell 11 and a second shell 12, the rear side of the first shell 11 and the front side of the second shell 12 are detachably connected, the "front side" and "front end" of the urine test instrument 100 are the side of the first shell 11 away from the second shell 12, and the "rear side" or "rear end" is the side of the second shell 12 away from the first shell 11. The reagent box 2, the rotor 6 and the flow channel plate 3 are located in the first shell 11, and the pump and the driving member 93 are located in the second shell 12, and the driving member 93 and the rotor 6 are detachably connected. The driving member 93 and the pump in the second shell 12 are durable parts with a long service life, while the first shell 11 and the reagent box 2, the rotor 6 and the flow channel plate 3 in the first shell 11 are combined into a detection device, which is a consumable equipment. After a period of use, the reagent in the reagent box 2 is consumed, and the detection device can be replaced, which can ensure that the materials are used to the fullest and reduce waste.

[0049] In a specific embodiment, Figure 4-5 As shown, the first housing 11 includes a first front cover 111 and a first rear cover 112, which are snap-fitted or fixedly connected by bolts to form a receiving space. As shown, the first front cover 111 includes a first front connecting ring 1111 and a first front cover plate 1112 connected to the front end of the first front connecting ring 1111. The first rear cover 112 includes a first rear connecting ring 1121 and a first rear cover plate 1122 connected to the rear end of the first rear connecting ring 1121, the rear end of the first front connecting ring 1111 and the front end of the first rear connecting ring 1121 are opposite to each other and snap-fitted, and are also fixedly connected by bolts, and the specific connection method is not limited.

[0050] like Figure 4 As shown, the second housing 12 includes a second front cover 121 and a second rear cover 122 . The second front cover 121 and the second rear cover 122 are fixedly connected to form another accommodating space for accommodating the pump and the driving member 93 .

[0051] The front end of the second front cover 121 is provided with a recessed mounting groove 1211. Figure 2 As shown, the mounting groove 1211 is used to accommodate the first rear cover 112 .

[0052] In addition, if Figure 8 As shown, the urine testing instrument 100 also includes two locking members 13, which are respectively connected to the first rear connecting ring 1121 and symmetrically arranged on both sides of the first rear connecting ring 1121. The two locking members 13 can detachably connect the first rear cover 112 and the second front cover 121 of the second shell 12.

[0053] Specifically, Figure 6-9As shown, the side wall of the mounting groove 1211 of the second front cover 121 is provided with two locking grooves 1212 arranged at intervals, and the two locking members 13 respectively include a locking tongue 131, a rotating member 132 and a handle 133. The rotating member 132 is rotatably connected to the first rear cover plate 1122 of the first rear cover 112 through a rotating shaft, and the locking tongue 131 is connected to the rear end of the rotating member 132, and the handle 133 is also connected to the front end of the rotating member 132. like Figure 7 As shown, the first front connecting ring 1111 is provided with a handle avoidance hole 1113, the handle 133 is plate-shaped and the inner end is connected to the front end of the rotating member 132, and the outer end extends to the outside of the first front connecting ring 1111 through the handle avoidance hole 1113. Figure 2 , Figure 6 As shown, the first rear connecting ring 1121 is provided with a lock tongue avoidance hole 1123 , the inner end of the lock tongue 131 is connected to the rotating member, and the outer end extends into the locking groove 1212 through the lock tongue avoidance hole 1123 and is engaged with the locking groove 1212 .

[0054] like Figure 2 , Figure 6 As shown, when the first housing 11 needs to be disassembled, the handle 133 is pressed toward the inside of the first housing 11 to rotate the rotating member 132, and the locking tongue 131 can be removed from the locking groove 1212. When installing, the handle 133 is rotated in the opposite direction to lock the locking tongue 131 into the locking groove 1212.

[0055] In addition, two stoppers 1124 are provided on the first rear connecting ring 1121. Figure 7 As shown, the two limit members 1124 are located at both ends of the handle avoidance hole 1113, and two protrusions 134 are also provided at the inner end of the handle 133. The two protrusions 134 are located in the first rear connecting ring 1121 and are blocked by the two limit members 1124 to prevent the handle 133 from detaching from the first rear connecting ring 1121.

[0056] In other embodiments, an elastic member may be provided between the rotating member 132 and the inner wall of the first rear connecting ring 1121, and the elastic member may pull the locking tongue 131 of the rotating member 132 to move toward the locking groove 1212 to prevent the locking tongue 131 from automatically disengaging from the locking groove 1212. Of course, in other embodiments, the locking tongue 131 and the locking groove 1212 may be interference-fitted to lock the locking tongue 131.

[0057] It should be understood that in other embodiments, the first shell 11 and the second shell 12 may be snap-fitted or magnetically connected, or bolted, and the present invention does not limit the specific connection method of the first shell 11 and the second shell 12.

[0058] The reagent box 2 is installed in the first housing 11 and is provided with a plurality of reagent chambers 21, and the reagent chambers 21 are used to contain reagents. Figure 10-11 As shown, the reagent box 2 is in the shape of a flat plate and is provided with a plurality of reagent chambers 21 and a plurality of flow channel holes 22. The plurality of reagent chambers 21 extend in the vertical direction and are arranged in the first direction, which is the width direction of the urine test instrument 100. The reagent box 2 is in the shape of a plate and is stacked with the flow channel plate 3. The outer periphery of the reagent box 2 is substantially in contact with the inner wall of the first shell 11. The plurality of reagent chambers 21 substantially fill the entire reagent box 2, and can hold more reagents without taking up too much space.

[0059] like Figure 6 , Fig.10 As shown, the first front connecting ring 1111 and the first rear connecting ring 1121 of the first shell 11 are both annular, that is, the inner wall of the first shell 11 is an arc surface, the bottom end of the reagent reagent 2 is also arc-shaped, and the bottom ends of the multiple reagent chambers 21 are arranged around a horizontal axis and are basically on the same circumference. The bottom ends of the multiple reagent chambers 21 are close to the bottom end of the reagent reagent 2 to increase the utilization rate of the reagent chambers 21.

[0060] like Fig.10 As shown, the bottom wall of the reagent chamber 21 includes an inclined surface 23 and a vertical surface 24 . The vertical surface 24 extends in the vertical direction, and the inclined surface 23 is a slope that gradually decreases from back to front. The bottom end of the inclined surface 23 is connected to the top surface of the vertical surface 24 .

[0061] The top and front side of the reagent chamber 21 are open, and the reagent can be put in from the top, and then a seal is set at the top of the reagent chamber 21, and an oil seal can also be used. A sealing plate (not shown) is also installed at the front end of the reagent chamber 2, and the sealing plate is superimposed on the front side of the reagent chamber 2 to seal the reagent chamber 21. Of course, in the case of convenient processing, the sealing plate can also be not set, and the front side of the sealed chamber can be set with a sealing shape.

[0062] like Fig.10 and Fig.11 As shown, the plurality of flow channel holes 22 are also arranged at intervals around the horizontal axis and are recessed from the rear side of the reagent box 2 toward the front end, and the plurality of flow channel holes 22 are recessed to the vertical surface 24 and communicated with the plurality of reagent chambers 21. The inclined surface 23 is provided to facilitate the reagent to slowly flow into the bottom end and flow into the flow channel plate 3 from the flow channel holes 22.

[0063] like Fig.10 and Fig.11 As shown, the rear side of the reagent box 2 is further provided with a plurality of sealing grooves 25, which are formed by the recess of the rear side of the reagent box 2, and the plurality of flow channel holes 22 are formed by the recess of the plurality of sealing grooves 25 away from the inner wall of the second housing 12 to the vertical surface 24. The sealing grooves 25 can also be set to be tapered, that is, the sealing grooves 25 gradually decrease from the back to the front, and the external opening is larger.

[0064] like Figure 7 , Fig.12As shown, the microfluidic chip includes a flow channel plate 3 and a cover plate 7 stacked on the front side of the flow channel plate 3, wherein the cover plate 7 is located on the rear side of the reagent box 2, and the cover plate 7 is in the shape of a thin plate and is stacked on the rear side of the reagent box 2 and the front side of the flow channel plate 3. A plurality of conical plug-in posts 71 are provided on the front side of the cover plate 7, and the plurality of plug-in posts 71 are respectively located in the plurality of sealing grooves 25, and a plurality of sealing rings are sleeved outside the plurality of plug-in posts 71, so that the plurality of plug-in posts 71 and the plurality of sealing grooves 25 are sealed and connected.

[0065] like Figure 7 , Fig.12 As shown, the rear side of the cover plate 7 is also provided with a plurality of through holes 72, which extend to the front ends of the plurality of plug-in posts and are respectively connected to the plurality of flow channel holes 22, and the reagents in the reagent chamber 21 can flow into the flow channel plate 3 through the flow channel holes 22 and the through holes 72 of the plug-in posts 71.

[0066] The cover plate 7 and the reagent box 2 are connected by snapping or bonding, or by thermoplastic means. The present invention does not limit the specific connection method of the cover plate 7 and the reagent box 2.

[0067] exist Figure 11-13 In the illustrated embodiment, a slot 26 is provided at the bottom end of the reagent box 2, and a protruding buckle 73 is provided at the bottom end of the cover plate 7. The buckle 73 can be inserted into the slot 26 of the reagent box 2, so that the reagent box 2 and the cover plate 7 can be quickly assembled.

[0068] The flow channel plate 3 is stacked on the rear side of the cover plate 7. The flow channel plate 3, the cover plate 7 and the reagent box 2 are fixedly connected to the first front cover plate 1112 or the first rear cover plate 1122 of the first housing 11 by the same bolt. Figure 4 and Figure 7 In the illustrated embodiment, the inner walls of the first front cover plate 1112 and the first rear cover plate 1122 of the first shell 11 are respectively provided with two mounting posts 14, and the mounting posts 14 of the first front cover plate 1112 and the first rear cover plate 1122 abut against each other and are both provided with threaded holes, and the threaded holes of the mounting posts 14 of the first rear cover plate 1122 extend to the rear side of the first rear cover plate 1122, and bolts can be screwed into the threaded holes of the mounting posts 14 of the first front cover plate 1112 from the rear side of the first rear cover plate 1122, thereby fixing the first rear cover plate 1122 and the first front cover plate 1112.

[0069] In addition, if Figure 7As shown, the reagent box 2 is provided with a countersunk hole 27, and the first rear cover plate 1122 is also provided with another mounting column 14, the threaded hole of the mounting column 14 is arranged opposite to the countersunk hole 27, and the inner end of the mounting column 14 is also provided with a flange, a part of which can abut against the rear side of the flow channel plate 3. By screwing the bolt into the countersunk hole 27 from the rear side of the first rear cover plate 1122, the reagent box 2, the cover plate 7 and the flow channel plate 3 can be fixed together on the first rear cover plate 1122, and the reagent box 2, the cover plate 7 and the flow channel plate 3 can be tightly fitted.

[0070] The front side of the flow channel plate 3 is provided with a central hole 31, a plurality of circumferential holes 32, a common flow channel 33, a sample flow channel, a water flow channel, an air flow channel 36, a plurality of reagent flow channels 37 and a detection flow channel 38. Figure 14-16 As shown, the central hole 31 is located on the horizontal axis, and the central hole 31 is a through hole 72 that runs through the front and back of the flow channel plate 3. The plurality of circumferential holes 32 are located on a circle centered on the central hole 31 and are arranged at intervals, that is, the plurality of circumferential holes 32 are on the same circle.

[0071] The inner end of the sample flow channel, the inner end of the clean water flow channel, the inner end of the air flow channel 36, the inner end of the detection flow channel 38 and the inner ends of the multiple reagent flow channels 37 respectively extend to the multiple circumferential holes 32 and are connected with the multiple circumferential holes 32, while the outer ends of the sample flow channel, the outer end of the clean water flow channel, the outer end of the air flow channel 36, the outer end of the detection flow channel 38 and the outer ends of the multiple reagent flow channels 37 are respectively dispersed away from the central hole 31 to other positions of the flow channel plate 3, wherein the outer end of the detection flow channel 38 is connected with the detection cavity of the detection mechanism to facilitate the sample and reagent to flow from the detection flow channel 38 into the detection cavity, and the outer ends of the multiple reagent flow channels 37 respectively extend to another circumference and are aligned and connected with the multiple through holes 72 of the multiple cover plates 7.

[0072] In some embodiments, a clean water flow channel and a sample flow channel are respectively provided, and the outer end of the clean water flow channel is connected to the clean water collection chamber 412 of the collection chamber through a pipe, so that water can enter the clean water flow channel from the clean water collection chamber 412. The sample flow channel is connected to the sample collection chamber 411 through a pipe, so that the sample can enter the sample flow channel from the sample collection chamber 411.

[0073] exist Fig.14 and 16 In the illustrated embodiment, two clean water channels and two sample channels are provided, and the inner ends of the two clean water channels and the two sample channels are respectively connected to different circumferential holes 32. The use of the two clean water channels and the two sample channels will be described in detail below.

[0074] In order to facilitate the communication between the sample flow channel, the detection flow channel 38, the reagent flow channel 37 and the air flow channel 36 and the sample collection chamber 411, the detection chamber and the reagent chamber 21, a first adapter 301 is also installed on the rear side of the flow channel plate 3. The first adapter 301 can be installed in the first shell 11 or in the second shell 12.

[0075] Specifically, if Fig.18 and Fig.19 As shown, if the first adapter 301 is installed in the first shell 11, the first adapter 301 is clamped or fixedly connected to the rear side of the flow channel plate 3 by bolts, or welded, etc., and the specific connection method between the first adapter 301 and the rear side of the flow channel plate 3 is not limited.

[0076] The first adapter 301 is used to introduce samples and clean water into the microfluidic chip, and also discharge the waste liquid after detection in the detection board into the collector, and then from the collector to the toilet. Moreover, after the reagent is used up, the first adapter 301 and the pipeline are disassembled together, and all the components in the first shell are directly replaced with new ones, which reduces pollution and increases the service life of the urine tester.

[0077] like Fig.18 , Fig.26 , Fig. 27 As shown, the first adapter 301 is provided with seven adapter tubes, which are used to connect with the sample collection chamber 411, the clean water collection chamber 412, the detector's drainage channel 416 or the external air through multiple pipes. The adapter tubes are preferably arranged at the top of the first adapter 301 to facilitate the sample or water to flow from the sample collection chamber 411 or the clean water collection chamber 412 into the flow channel plate 3, and also to facilitate the liquid in the detection chamber to flow from the connecting tube and the pipeline into the collector's drainage channel 416.

[0078] like Fig.18 , Fig.26 , Fig. 27 As shown, among the seven transfer tubes, two of them are connected to the sample collection chamber 411 of the collector, and the transfer tubes are defined as sample transfer tubes 3011. The other two transfer tubes are connected to the clean water collection chamber 412 of the collector, and the two transfer tubes are defined as clean water transfer tubes 3012. Another transfer tube is connected to the air flow channel 36 of the flow channel plate 3, and is defined as air transfer tube 3013. Another transfer tube is connected to the sewage discharge channel 416 of the collector, and is defined as sewage discharge transfer tube 3014. The last transfer tube is connected to the drive pipeline, and is defined as drive transfer tube 3015.

[0079] like Fig.18 As shown, the front side of the first adapter 301 is provided with seven first slots 3016 , and the first adapter 301 is provided with seven channels, and the multiple channels extend toward and communicate with the seven adapter tubes. The bottom ends of the seven channels are communicated with the multiple first slots 3016 .

[0080] like Fig.14 , Fig.17 As shown, the rear side of the flow channel plate 3 is also provided with nine first adapter posts 3017, and the nine first adapter posts 3017 are respectively provided with first adapter holes, and the nine first adapter holes extend into the flow channel plate 3 and are connected with the clean water flow channel, the detection flow channel 38, the sample flow channel or the air flow channel 36 of the flow channel plate 3. Seven of the nine first adapter posts 3017 are respectively inserted into the seven first slots 3016 of the first adapter, and the other two need to be inserted into the inlet and outlet of the detection plate. Among the seven first adapter posts, the first adapter holes of two first adapter posts 3017 are connected with the two sample flow channels of the flow channel plate 3, and the first adapter holes of the two first adapter posts 3017 are also connected with the two sample adapter tubes 3011. The first adapter holes of the other two first adapter posts 3017 are connected with the two clean water flow channels and are also connected with the two clean water transfer tubes 3012.

[0081] like Fig.14 , Fig.17 , Fig.18 As shown, the first transfer hole of one of the first transfer posts 3017 is connected to the air flow channel 36 and the air transfer tube 3013. The first transfer hole of another first transfer post 3017 is connected to the drive pipeline and the drive transfer tube 3015. There are three first transfer holes of the first transfer posts 3017, one of which is used to communicate with the sewage transfer tube 3014, and the other two are used to communicate with the detection chamber respectively, which will be described in detail below.

[0082] In short, the seven first adapter columns 3017 are respectively inserted into the seven first slots 3016 of the first adapter, so that the outer end of the sample flow channel of the flow channel plate 3 is connected to the sample transfer tube 3011, the outer end of the clean water flow channel is connected to the clean water transfer tube 3012, the outer end of the air flow channel 36 is connected to the air transfer tube 3013, and the outer end of the detection flow channel 38 is connected to the sewage transfer tube 3014.

[0083] In another embodiment, the first adapter 301 is installed in the second shell 12 and can also be installed in the installation groove. Since the second shell 12 and the first shell 11 are detachably connected, no matter whether the first adapter 301 is connected to the inside or outside of the second shell 12, the multiple first slots 3016 of the first adapter 301 need to be exposed in the installation groove 1211 of the second shell 12.

[0084] like Fig.17As shown, the rear side of the flow channel plate 3 is also provided with a plurality of first adapter posts 3017, and the first rear cover plate 1122 of the first shell 11 also needs to be provided with avoidance holes for avoiding the plurality of first adapter posts 3017, so as to facilitate the first adapter posts 3017 to be inserted into the first slots 3016 of the first adapter 301 as the first shell 11 and the second shell 12 are assembled.

[0085] like Figure 6 , Fig.17 , Fig.18 As shown, when the first housing 11 and the second housing 12 are assembled, when the locking tongue 131 of the locking member 13 penetrates into the locking groove 1212 , the plurality of first transfer posts 3017 can be squeezed into the plurality of first slots 3016 at the same time.

[0086] Of course, in order to increase the sealing performance between the first adapter 301 and the flow channel plate 3 , a sealing ring sleeved on the first adapter column 3017 needs to be provided in the first slot 3016 .

[0087] like Fig.18 , Fig.19 As shown, the detection plate 5 can be installed in the first shell 11 and fixedly connected or clamped to the first adapter 301, or fixedly connected to the rear side of the flow channel plate 3. Of course, in other embodiments, the detection plate 5 can also be arranged in the second shell 12 and be arranged to be detachably connected to the first adapter 301.

[0088] Fig.14 , 19 As shown, if it is arranged in the first housing 11, the detection plate 5 can be directly fixedly connected to the rear side of the flow channel plate 3, one end of the detection cavity is connected to the detection flow channel 38, and the other end is connected to the sewage transfer pipe 3014 of the first adapter 301, and the sewage transfer pipe 3014 is connected to the sewage channel 416 of the collector through a pipeline. The liquid in the detection flow channel 38 flows into the detection cavity for detection, and after the detection is completed, the liquid in the detection cavity can flow from the sewage transfer pipe 3014 into the sewage channel 416, and be discharged from the sewage channel 416 into the toilet.

[0089] Specifically, Figure 5 , Fig.19 , Fig.21 As shown, the detection plate 5 is a plate extending forward and backward along the urine tester 100, and the detection cavity is roughly U-shaped and has an inlet 51 and an outlet 52, and the inlet 51 and the outlet 52 are respectively located at the front end of the detection plate 5. Fig.17In the embodiment, there are three first adapter posts 3017 on the rear side of the flow channel plate 3, two of which are inserted into the inlet 51 and the outlet 52 respectively. The first adapter posts 3017 inserted into the inlet 51 connect the detection flow channel 38 of the flow channel plate 3 with the inlet 51, and the sample and liquid in the detection flow channel 38 can flow into the detection cavity from the inlet 51 in sequence for detection. In other words, one end of the detection cavity is connected to the detection flow channel, and the other end is connected to the sewage pipe.

[0090] like Fig.14 , Fig.17 , Fig.18 As shown, the flow channel plate 3 is also provided with a sewage flow channel 302, and the two ends of the sewage flow channel 302 are respectively connected with the other two first adapter posts 3017 on the rear side of the flow channel plate 3. One of the two first adapter posts 3017 is inserted into the outlet 52 of the detection chamber, and the first adapter hole of the first adapter post 3017 is connected with the outlet 52. The other first adapter post 3017 is inserted into the first slot 3016 at the front end of the first adapter 301, and is connected with the sewage transfer tube 3014 located at the top of the first adapter 301.

[0091] That is to say, the mixed liquid of the sample and reagent in the detection channel 38 flows into the detection chamber from the first adapter hole of one of the first adapter columns 3017 and the inlet 51 of the detection chamber. After the detection is completed, the mixed liquid flows into the sewage channel 302 of the flow channel plate 3 from the outlet 52, and then flows from the sewage channel 302 into the sewage transfer tube 3014, and then flows from the sewage transfer tube 3014 and the pipeline into the sewage channel 416 of the collection chamber, and then flows from the sewage channel 416 into the toilet.

[0092] The function of the sewage flow channel 302 is equivalent to the flow channel for transfer, one end of which is connected to the flow outlet 52 of the detection chamber, and the other end is connected to the sewage transfer pipe 3014. The mixed liquid in the detection chamber passes through the sewage flow channel 302 and then flows into the sewage channel 416 from the sewage transfer pipe 3014. This design can reduce the volume of the first adapter 301 and make full use of the internal space of the flow channel plate 3 and the first adapter 301.

[0093] If the detection board 5 is installed in the first housing 11, when the reagent in the first housing 11 is consumed, it can be removed along with the first housing 11. When a new detection device is replaced, a new detection board 5 is also installed inside it.

[0094] The detector of the present invention includes a light source device and a photosensitive sensor, which are respectively arranged at corresponding positions of the detection board 5. The detection board 5 is made of transparent material, and the light source device is an LED light, which is used to illuminate the mixed liquid in the detection cavity, and the photosensitive sensor is used to sense the light after the light source device illuminates the mixed liquid to detect the sample components. However, the installation of the detector is relatively complicated, so in order to facilitate customer assembly, the detector needs to be installed in the second shell 12, that is, the detector and the detection board 5 are assembled separately. The detector needs to be close to the detection board 5 to detect the mixed liquid in the detection cavity, so the rear end of the detection board 5 needs to extend into the second shell 12.

[0095] Specifically, if Fig. 20 As shown, avoidance holes need to be provided on the inner walls of the first rear cover plate 1122 of the first shell 11 and the mounting groove 1211 of the second shell 12, respectively. The avoidance holes are detection plate avoidance holes 15, and the front end of the detection plate 5 is connected to the flow channel plate 3 and the first adapter 301, and the rear end extends from the two detection plate avoidance holes 15 of the first shell 11 and the second shell 12 to the second shell 12, and the detector is installed at the top or bottom of the detection plate avoidance hole 15 of the second shell 12, so as to detect the liquid in the detection cavity.

[0096] In another embodiment, the detection plate 5 can also be directly installed in the second shell body 12, and the front end of the detection plate 5 extends to the inner wall of the installation groove 1211, and among the three first adapter columns 3017 at the rear end of the flow channel plate 3, the first adapter holes of two of the first adapter columns 3017 are respectively connected to the two ends of the sewage flow channel 302, and the first adapter hole of one of the two first adapter columns 3017 is connected to the sewage adapter tube 3014, and the other is connected to the flow outlet 52 of the detection chamber.

[0097] Among the three first adapter posts 3017 , the first adapter hole of another first adapter post 3017 is communicated with the inlet 51 of the detection chamber.

[0098] The two first adapter columns 3017 connected to the inlet 51 and the outlet 52 of the detection chamber need to extend to the rear side of the first rear cover plate 1122 of the first shell 11. When the first shell 11 and the second shell 12 are assembled, the rear ends of the two first adapters 301 can be inserted into the two inlet 51 and the outlet 52 at the front end of the detection plate 5, and quick disassembly and assembly can also be achieved.

[0099] It should be understood that a sealed connection is required between the multiple adapter tubes and the multiple first adapter posts 3017 or between the first adapter posts and the outflow ports 52 and the inflow ports 51 , and the sealing method may be a sealing ring or other methods.

[0100] The common flow channel 33 is a curved flow channel, and the inner end extends to the center hole 31 and is connected to the center hole 31, and the outer end goes around the center hole 31 in a circle formed by the multiple circumferential holes 32 and then bends and extends to the outside of the flow channel plate 3. The common flow channel 33 and the multiple circumferential holes 32 are staggered and are not directly connected to each other.

[0101] In one embodiment, the outer end of the common flow channel 33 is used to communicate with the driving pipe, and the driving pipe is located in the second housing and connected to a pump, and the pump can pump out the air in the driving pipe and the common flow channel 33. Of course, in some embodiments, two pumps can be arranged in series through a pipeline, and one of the pumps is connected to the driving pipe.

[0102] exist Fig.14 In the illustrated embodiment, at least one bend is provided in the middle portion of the common flow channel 33, which increases the volume of the common flow channel 33 on the one hand and prevents the reagent or sample in the common flow channel 33 from flowing back into the driving pipeline on the other hand.

[0103] The common flow channel 33 needs to be connected to the driving pipeline, and the pump is connected to the driving pipeline. After the pump is started, the air in the common flow channel 33 can be emptied to form a negative pressure.

[0104] The pump and the driving pipe are respectively located in the second housing 12 , so in order to quickly disassemble and assemble the driving pipe and the common flow channel 33 , a second adapter 306 needs to be installed. The second adapter 306 will be introduced below.

[0105] As a preferred solution, in order to more accurately and quantitatively extract reagents and samples, two pumps can be used to communicate with the common flow channel through two drive pipes, one is a peristaltic pump 91, and the other is a plunger pump 92. The two pumps are respectively installed in the second housing 12 and are respectively connected to the two drive pipes. The two drive pipes are defined as a first drive pipe and a second drive pipe.

[0106] The second drive pipeline is connected to the plunger pump, and the first drive pipeline is connected to the peristaltic pump 91. The first drive pipeline includes two sections, both ends of which are respectively connected to both ends of the hose of the peristaltic pump, and the other two sections are respectively connected to the two peristaltic pump adapter columns 3041 below.

[0107] The front side of the flow channel plate 3 also has a driving flow channel 303 , which extends in the vertical direction and has a top end connected to a first transfer hole of one of the first transfer columns 3017 , and the first transfer hole is connected to the driving transfer tube 3015 .

[0108] Three second transfer posts are further provided on the rear side of the flow channel plate 3 . The three second transfer posts are respectively provided with second transfer holes. Among the three second transfer posts, two are peristaltic pump transfer posts 3041 , and the other is a plunger pump transfer post 3042 .

[0109] like Fig.14 , Fig.17 As shown, the second transfer hole of one of the two peristaltic pump transfer columns 3041 is connected to the bottom end of the driving channel 303 , and the second transfer hole of the other peristaltic pump transfer column 3041 is connected to the common channel 33 .

[0110] The plunger pump adapter post 3042 is located between the two peristaltic pump adapter posts 3041 and its second adapter hole is also connected to the common flow channel 33 .

[0111] Fig.17 , Fig.18 As shown, the second adapter 306 is connected to the inner wall of the mounting groove 1211 of the second shell 12, and is provided with three second slots 3061. Among the three second slots 3061, two of the second slots 3061 respectively accommodate two peristaltic pump adapter columns 3041, that is, the common flow channel 33 is connected to one end of one section of the first drive pipeline through a peristaltic pump adapter column 3041, and one end of the other section of the first drive pipeline is connected to the bottom end of the drive flow channel 303 through another peristaltic pump adapter column 3041, and the top of the drive flow channel 303 is connected to the drive adapter tube 3015 through the first connecting column, and the drive adapter tube 3015 can also be connected to the collector. In other words, one end of the two sections of the first drive pipeline is respectively connected to the two ends of the hose of the peristaltic pump, and the other end is respectively connected to the common flow channel and the drive adapter tube 3015. After the peristaltic pump is started, the fluid in the common flow channel can be pumped from the first drive pipeline and the drive adapter tube to the collector, and discharged from the collector into the toilet.

[0112] Among the three second slots 3061 , another second slot 3061 is communicated with one end of the second driving pipe, and the second slot 3061 accommodates the plunger pump adapter column 3042 .

[0113] The peristaltic pump 91 is used to drive the air out of the common flow channel 33, or drive the sample and water in the sample collection chamber into the sample flow channel and the clean water flow channel, that is, a larger air flow is required, so a driving flow channel 303 is set on the flow channel plate 3, and a driving adapter pipe 3015 is set on the first adapter 301, which can provide a larger air flow if it is bent. The plunger pump 92 only needs to quantitatively extract the sample and reagent from the sample flow channel and the reagent flow channel 37 to the common flow channel, and the required flow is relatively small, so one end of the second driving pipe is connected to the common flow channel 33, and the other end is set near the plunger pump 92.

[0114] like Figure 5As shown, the detection channel 38 is connected to the detection cavity, and a mixing cavity 39 is also provided on the path of the detection channel 38. The mixing cavity 39 is connected to the detection channel 38 and the width of the mixing cavity 39 is greater than the diameter of the detection channel 38. When the sample and the reagent flow in the detection channel 38, they pass through the mixing cavity 39. Since the width of the mixing cavity 39 is large, the sample and the reagent will be accelerated when flowing from the detection channel 38 into the mixing cavity 39, and then mixed in the mixing cavity 39.

[0115] As a preferred embodiment, the mixing chamber 39 includes a mixing bend 392 and at least one mixing chamber 391 that are connected to each other. The mixing bend 392 is an S-shaped curved flow channel, and the mixing chamber 391 is connected to the mixing bend 392 and the width of the mixing chamber 39 is greater than the diameter of the mixing bend 392 and the detection flow channel 38.

[0116] exist Figure 14-16 In the illustrated embodiment, the mixing chamber 39 includes three mixing bends 392 and three mixing chambers 391. The three mixing chambers 391 and the three mixing bends 392 are alternately arranged and connected to each other, and the inlet of the mixing chamber 39 is at the bottom and the outlet is at the top. That is, the three mixing bends 392 and the mixing chamber 39 are curved and extend upward.

[0117] The mixing bend 392 includes at least two horizontal flow channels and at least one turning flow channel 394, and any one of the turning flow channels 394 is located at both ends of the two horizontal flow channels; The mixing bend 392 is an S-shaped bend and includes at least two horizontal flow channels and at least one turning flow channel 394 . The horizontal flow channel is a flow channel extending in the horizontal direction, and the turning flow channel 394 is located between any two horizontal flow channels, that is, at the corner of the mixing bend 392 .

[0118] like Fig.16 As shown, the turning channel 394 includes a curved inner arc 395 and an outer arc 396 , both of which are arc-shaped and the inner diameter of the inner arc 395 is smaller than the inner diameter of the outer arc 396 .

[0119] The mixing chamber 39 of the present invention adopts an S-shaped mixing bend 392 and a mixing chamber 39, and performs an outer diameter enlargement process at the turning corner of the mixing bend 392. By utilizing the bend with a variable diameter here, the flow velocity at the outer arc 396 is greater than the flow velocity at the inner arc 395, forming a local Dean flow effect, thereby improving the mixing effect of the mixed liquid of the sample and the reagent in the flow channel.

[0120] like Fig.15As shown, the cross-section of the mixing chamber 391 along the vertical direction is roughly circular, and the two side walls of the mixing chamber 391 are also arc surfaces, which are a first arc surface 397 and a second arc surface 398 opposite to the first arc surface 397. Both the first arc surface 397 and the second arc surface 398 are arc surfaces protruding toward the outside.

[0121] As a preferred solution, Fig.15 As shown, the first arc surface 397 and the second arc surface 398 are arc surfaces with the same diameter and the same arc length, and the tangents at both ends of the first arc surface 397 and the second arc surface 398 are parallel to each other. In the embodiment shown in the figure, the arc length of the first arc surface 397 is equal to 1 / 4 of the circumference, and the tangents at both ends are perpendicular to each other, and one of the tangents extends in the vertical direction and the other extends in the horizontal direction.

[0122] Since the entire mixing chamber 39 is bent and extended in the vertical direction, Fig.16 As shown, each mixing chamber 391 is located between two mixing bends 392, and each mixing chamber 39 needs to be connected to the horizontal flow channels of the two mixing bends 392. The two horizontal flow channels are respectively arranged in mirror symmetry, one of which is located at the top of the mixing chamber 391, and the other is located at the bottom of the mixing chamber 391.

[0123] Among the two horizontal flow channels connected to the mixing chamber 391, one of the horizontal flow channels is defined as the liquid inlet horizontal flow channel 3931, and the other is defined as the liquid outlet horizontal flow channel 3932. The liquid inlet horizontal flow channel 3931 is connected to the top of the mixing chamber 391, and the liquid outlet horizontal flow channel 3932 is connected to the bottom of the mixing chamber 391. The sample or reagent flows into the mixing chamber 391 from the liquid inlet horizontal flow channel 3931, and then flows out from the liquid outlet horizontal flow channel 3932. That is to say, the liquid inlet horizontal flow channel 3931 is located upstream of the liquid outlet horizontal flow channel 3932, and the sample and reagent flow into the detection flow channel 38 from the common flow channel 33, and after entering the mixing chamber 391 from the liquid inlet horizontal flow channel 3931 of a mixing bend 392, for example, and then flows into the horizontal flow channel of another mixing bend 392.

[0124] like Fig.15 As shown, the mixing chamber 391 has an inlet 3911 and an outlet 3912, wherein the inlet 3911 is connected to the inlet horizontal flow channel of one of the mixing bends, and the outlet 3912 is connected to the outlet horizontal flow channel 3932 of another mixing bend. The sample and reagent can flow into the mixing chamber from the inlet horizontal flow channel of one of the mixing bends and the inlet 3911, and then flow into another mixing bend from the outlet 3912 and the outlet horizontal flow channel, and flow continuously in several mixing chambers and several mixing bends.

[0125] The connecting line between the inlet horizontal flow channel 3931 and the outlet horizontal flow channel 3932 is used as the dividing line. Fig.15 The dashed line shown is a dividing line. The dividing line can divide the mixing chamber 391 into two parts, wherein the area formed by the dividing line and the first arc surface 397 located below is the slow area 3934, and the area between the dividing line and the second arc surface 398 is the fast area 3933. The fast area 3933 and the slow area 3934 flowing into the mixing chamber 391 from the liquid inlet horizontal flow channel 3931 have a certain speed difference, and the mixed liquid of the sample and the reagent forms a vortex around the center point in the circular mixing chamber 391, which increases the contact area between the liquids and improves the mixing effect.

[0126] As the liquid outlet horizontal flow channel 3932 becomes narrower, the speed of the mixed liquid of the sample and the reagent increases suddenly when it flows out of the liquid outlet horizontal flow channel 3932, and the mixed liquid of the sample and the reagent is relatively squeezed, which once again increases the mixing strength of the mixed liquid of the sample and the reagent. By connecting the above-mentioned multiple diameter-changing mixing bends 392 and the circular mixing chamber 391 in series, the mixing strength is gradually increased until the detection cavity, the sample and the reagent are basically in a completely mixed state.

[0127] The mixing chamber 39 of the present invention is often used in the fields of microfluidic chip technology such as biology and chemistry. When samples or reagents or samples and reagents need to be mixed, this structure can greatly improve the mixing efficiency and avoid some residue and bubble problems.

[0128] In the mixing cavity 39, the liquid inlet horizontal flow channel 3931 connected to each mixing chamber 391 is arranged relatively higher than the liquid outlet horizontal flow channel 3932. In special application scenarios, such as when it needs to be used vertically, when the liquid flows from bottom to top, there is no need to consider the influence of gravity, so that no residual problem will occur in the mixing chamber 391.

[0129] When samples or reagents flow in a relatively rough flow channel, bubbles are easily generated, which affects the mixing effect and may even be brought into the detection cavity, causing analysis errors. The multiple mixing cavities 39 of the core of the present application are connected in series with the mixing bends 392, and the flow channel corners of each mixing bend 392 are processed with smooth rounded transitions, which can avoid and eliminate bubbles in the mixing chamber 391 to a certain extent.

[0130] The inlet of the mixing chamber 39 is located below the outlet, and the mixing chamber 39 is located above the common flow channel 33. The inlet of the mixing chamber 39 is connected to the common flow channel 33, and the outlet is connected to the detection chamber. The sample and reagent in the common flow channel 33 flow into the mixing chamber 39 from the bottom inlet of the mixing chamber 39, and then flow into the detection chamber from the outlet.

[0131] like Fig.14 , Fig. 22As shown, the rotor 6 is rotatably mounted in the first shell 11 and is located on the rear side of the flow channel plate 3. A transfer channel is provided inside the rotor 6. The inner end 61 and the outer end of the transfer channel are open toward the front side of the rotor 6. The transfer channel is roughly U-shaped. The inner end 61 of the transfer channel is aligned with the center hole 31, and the inner end is recessed from the front side of the rotor 6 to the inside of the rotor 6. The outer end 62 of the transfer channel is also open toward the front side of the rotor 6, and the distance from the outer end 62 to the inner end 61 is the same as the distance between the center hole 31 and the multiple circumferential holes 32.

[0132] like Fig.14 , Fig. 22 As shown, the inner end 61 of the transfer channel is aligned with and connected to the central hole 31 , and the outer end 62 can rotate with the rotor 6 and can be aligned with and connected to any circumferential hole 32 as the rotor 6 rotates.

[0133] When it is necessary to extract a sample, the rotor 6 rotates so that the outer end 62 of the transfer channel is aligned with the circumferential hole 32 connected to the inner end of the sample flow channel. At this time, the two ends of the transfer channel are respectively connected to the sample flow channel and the common flow channel 33. The peristaltic pump 91 can extract the air in the common flow channel 33, the transfer channel, the sample flow channel, and the external sample transfer tube or the pipe connected to the transfer tube to form a negative pressure, thereby drawing the sample in the sample collection chamber 411 to the common flow channel 33.

[0134] When it is necessary to extract the reagent, the same operation is performed, and the rotor 6 is rotated so that the outer end 62 of the transfer channel is aligned with the circumferential hole 32 connected to the reagent flow channel 37, so that the common flow channel 33 is connected to the reagent channel, and the peristaltic pump 91 discharges the air in the common flow channel 33 and the reagent channel to form a negative pressure, and the reagent in the reagent chamber 21 is drawn into the common flow channel 33.

[0135] When it is necessary to extract the clean water in the clean water collection chamber 412, the same operation is performed and will not be described in detail.

[0136] After the sample and reagent are drawn into the common flow channel 33, the rotor 6 continues to rotate so that the outer end 62 of the transfer channel faces the circumferential hole 32 connected to the inner end of the detection flow channel 38, and the peristaltic pump 91 drives the sample and reagent in the common flow channel 33 to flow from the common flow channel 33 into the detection flow channel 38, and then from the detection flow channel 38 into the detection cavity.

[0137] In the embodiment of setting the mixing chamber 39, the peristaltic pump 91 can push the sample and reagent from the detection flow into the mixing chamber 39, and push the sample and reagent from the bottom entrance of the mixing chamber 39 to the top outlet. In order to achieve the mixing effect, the peristaltic pump 91 can also be used to repeatedly flow the sample and reagent back through the mixing chamber 391 and the mixing bend 392. After the sample and reagent pass through the mixing chamber 391 and multiple mixing bends 392, they are repeatedly mixed to form a mixed liquid until the mixing effect is achieved, and finally the mixed liquid flows into the detection chamber from the inlet 51 of the detection chamber. According to the above description, the flow channel plate 3 is also provided with a sewage flow channel 302, and the two ends of the sewage flow channel 302 are respectively connected to the first transfer holes of the other two first transfer posts 3017 on the rear side of the flow channel plate 3. One of the two first transfer posts 3017 is inserted into the flow outlet 52 of the detection chamber, and the first transfer hole of the first transfer post 3017 is connected to the outflow hole. The other first transfer post 3017 is inserted into the first slot 3016 at the front end of the first adapter 301, and is connected to the sewage transfer pipe 3014 located at the top of the first adapter 301.

[0138] After the detection is completed, the pump drives air into the detection channel 38 and the detection chamber, and the mixed liquid flows into the sewage channel 302 from the flow outlet 52 of the detection chamber, and then flows from the sewage channel 302 into the sewage transfer tube 3014, and flows from the sewage transfer tube 3014 and the pipeline into the sewage channel 416 of the collector, and then flows into the toilet through the sewage channel 416.

[0139] The rotor 6 is rotatably connected to the rear side of the flow channel plate 3 . In a specific embodiment, a connecting member 85 is further provided on the rear side of the flow channel plate 3 . The connecting member 85 is a ring extending around the outside of the plurality of circumferential holes 32 and is located radially outside the rotor 6 .

[0140] The outer side of the rotor 6 is also covered with a protective cover 81. Fig. 22 and 23 As shown, the protective cover 81 is covered outside the connecting member 85 and is also engaged with the connecting member 85 , and the rotor 6 rotates on the protective cover 81 .

[0141] In addition, if Fig. 22 , Fig.23 As shown, in order to drive the rotor 6 to abut against the rear side of the flow channel plate 3 and prevent the liquid from overflowing from the circumferential hole 32, a compression spring 82 is further provided in the protective cover 81, and a convex ring 83 is further provided on the rear side of the rotor 6. The compression spring 82 is sleeved on the outside of the convex ring 83 and the front and rear ends abut against the rear side of the rotor 6 and the rear wall of the protective cover 81, so that the rotor 6 and the flow channel plate 3 are closely matched. Of course, the compression spring 82 can also be replaced with other biasing members, such as magnets that attract each other are respectively provided on the rotor 6 and the flow channel plate 3.

[0142] The driving member 93 is located in the second housing 12 and is detachably connected to the rotor 6. Fig.24 As shown, the driving member 93 can be a motor. Of course, other driving modes can also be selected. The rotating shaft of the motor extends into the mounting groove 1211 of the second shell 12. After the second shell 12 and the first shell 11 are assembled, the rotating shaft and the rotor 6 of the motor can be automatically assembled.

[0143] Specifically, the first rear cover plate 1122 of the first housing 11 is provided with a clearance hole for avoiding the protective cover 81, and the inner wall of the mounting groove 1211 of the second housing 12 is also provided with a clearance hole for avoiding the motor shaft. The rear side of the protective cover 81 is located in the clearance hole of the first rear cover plate 1122 and is also provided with a through hole, and the shaft of the driving member 93 extends from the clearance hole on the inner wall of the mounting groove 1211 to the protective cover 81. In addition, the rear side of the convex ring 83 is provided with a concave spline groove 84, such as Fig.23 and 24 As shown, the front end of the motor shaft is provided with a spline 931 located in the spline groove 84. When the first housing 11 and the second housing 12 are assembled, the spline 931 of the shaft is inserted into the spline groove 84 to complete the assembly.

[0144] The collector is located outside the first shell 11 and the second shell 12 , and is connected to the adapter tube of the first adapter 301 through a plurality of pipes.

[0145] exist Figure 25-27 In the embodiment shown, the collector includes a collection plate 41, an outer cover plate 43, an inner cover plate 42 and a clean water collection member. The collection plate 41 is in a round shape and has an outer side and an inner side. The outer side is the side away from the toilet, and the inner side is the side close to the toilet. The outer side of the collection plate 41 is provided with a sample collection cavity 411, and the rear side is provided with a clean water collection cavity 412.

[0146] The sample collection chamber 411 is located at the top of the collection plate 41 and has a width that gradually increases from the bottom to the top and is open toward the top of the collection plate 41 for collecting samples. The clean water collection chamber 412 is located at the bottom of the collection plate 41 and is offset from the sample collection chamber 411 .

[0147] A water inlet channel 413, a water outlet channel 414, and a sample outlet channel 415 are also provided at the top of the collection plate 41. The water inlet channel 413 and the water outlet channel 414 are respectively connected to the clean water collection chamber 412. The water inlet channel 413 and the water outlet channel 414 are both located on the rear side of the collection plate 41 and are recessed from the top of the collection plate 41 into the clean water collection chamber 412.

[0148] The sample collection cavity 411 is located at the front side of the collection plate 41, and is formed by the groove formed by the depression of the front side of the collection plate 41. The collection plate 41 and the outer cover plate 43 are connected and assembled to form the sample collection cavity 411. The top of the front cover plate is provided with a filter part 44, which is a grille arranged at intervals at the top of the front cover plate, and a filter net or the like can also be added to filter foreign matter.

[0149] The inner walls on both sides of the sample collection cavity 411 are curved surfaces protruding toward each other, so that the width of the sample collection cavity 411 gradually increases from the bottom to the top. In addition, the curved inner wall can increase the opening of the sample collection cavity 411 and facilitate the sample to flow from the top to the bottom.

[0150] The inner wall of the sample collection cavity 411 is also provided with two concave drainage grooves 4111, which are respectively formed by being concave downward from the top of the collection plate 41 and are arranged at intervals, and a barrier column 402 is formed between the two drainage grooves 4111, and the barrier column 402 is basically located in the middle of the sample collection cavity 411. The two drainage grooves 4111 are also basically the same in shape. The bottom walls of the two drainage grooves 4111 are also curved surfaces that are concave downward.

[0151] The drainage groove 4111 is convenient for receiving more samples and for the samples to flow along the inner wall of the drainage groove 4111 , providing a larger flow channel arc surface, so that the samples can slowly flow into the bottom of the sample collection cavity 411 and reduce the generation of bubbles.

[0152] It should be understood that if the sample collection chamber 411 has enough space, multiple drainage grooves 4111 can be provided. The multiple drainage grooves 4111 can be arranged at intervals along the width direction of the collector. The inner walls and bottom walls of the multiple drainage grooves 4111 are preferably smooth curved surfaces.

[0153] A drain port 417 is also provided at the bottom of the sample collection cavity 411. The drain port 417 is open toward the bottom of the collection cavity and has a very small diameter. During the sample collection process, the drain port 417 is always in an open state. Due to its small diameter, it will not affect sample collection.

[0154] A liquid level sensor is also provided in the sample collection cavity 411 . The liquid level sensor is located below the two drainage grooves 4111 and adjacent to the drainage grooves 4111 , and is used to sense the liquid level of the sample collection.

[0155] In addition, the sample outlet channel 415 is formed by the recess at the rear side of the collection plate 41, and the bottom end of the sample outlet channel 415 extends into the sample collection cavity 411 and is located above the drain port 417, while the top end is located at the upper end of the collection plate 41, and the top end of the sample outlet channel 415 is connected to the sample transfer tube 3011 through a pipe. The sample in the sample collection cavity 411 can flow into the sample flow channel from the sample outlet channel 415 and the sample transfer tube 3011.

[0156] The clean water collection chamber 412 is formed by a depression at the rear side of the collection plate 41 . After the inner cover plate 42 is connected to the rear side of the collection plate 41 , the inner cover plate 42 covers the clean water collection chamber 412 to form a closed chamber.

[0157] The clean water collection chamber 412 includes a first chamber 4121 and a second chamber 4122. The first chamber 4121 and the second chamber 4122 are located on both sides of the sample collection chamber 411 and are connected at the bottom. The first chamber 4121 and the second chamber 4122 are symmetrically arranged, and exhaust holes 418 are respectively arranged at the top.

[0158] The water inlet channel 413 and the water outlet channel 414 are respectively formed by the recesses at the rear side of the collection plate 41 , and the bottom end of the sample outlet channel 415 is communicated with the bottom end of the sample collection cavity 411 .

[0159] The top of the water inlet channel 413 is also connected to an external clean water collection member, which can be a pipe connected to a clean water tank or a water switch, or a pipe directly attached to the inner wall of the toilet and located above the collector to collect clean water. The present invention does not limit the specific implementation of the clean water collection member.

[0160] The diameter of the water outlet channel 414 is much smaller than the diameter of the water inlet channel 413 , and the top of the water outlet channel 414 can be connected to the clean water transfer pipe 3012 through a pipeline.

[0161] That is to say, the water collected by the clean water collection member flows from the water inlet channel 413 into the clean water collection chamber 412, which is similar to a water storage tank. The water in the clean water collection chamber 412 then passes through the water outlet channel 414 and the clean water transfer tube 3012 into the clean water flow channel of the flow channel plate 3.

[0162] When water in the clean water collection piece enters the clean water collection chamber 412, bubbles will be generated, and the bubbles will float to the surface. The water outlet channel 414 is located at the bottom of the clean water collection chamber 412, and the water outlet channel 414 is relatively thin, so the bubbles will not enter the water outlet channel 414. After flowing into the clean water transfer pipe 3012 from the water outlet channel 414, no bubbles will be generated in the clean water that flows into the clean flow channel.

[0163] A liquid level sensor is also installed in the clean water collection chamber 412 to sense the liquid level of the clean water.

[0164] The rear side of the collection plate 41 is also provided with a drainage channel 416, which extends to the top of the collection plate 41 and is connected to the drainage transfer pipe 3014 through a pipe, and the drainage transfer pipe 3014 is connected to the detection chamber, and the inner cover plate 42 is also provided with a liquid outlet 421 connected to the drainage channel 416. After the detection is completed, the mixed liquid in the detection chamber can flow into the drainage channel 416 from the drainage channel 302 of the flow channel plate 3 and the drainage transfer pipe 3014 in sequence, and then flow into the toilet from the drainage channel 416 and the liquid outlet 421.

[0165] exist Figure 28-30 In another embodiment, the collector also includes a collecting plate 41, an outer cover plate 43, an inner cover plate 42 and a clean water collecting piece. Similarly, the collecting plate 41 is also in a round cake shape and is also provided with a clean water collecting chamber 412 and a sample collecting chamber 411. The top surface of the collecting plate 41 is also provided with a water inlet channel 413, a water outlet channel 414 and a sample outlet channel 415.

[0166] The difference is that the sample collection chamber 411 and the clean water collection chamber 412 are formed by splicing the collection plate 41, the inner cover plate 42 and the outer cover plate 43. The sample collection chamber 411 and the clean water collection chamber 412 are through grooves provided on the collection plate 41. After the front cover plate and the rear cover plate are covered on the front and back sides of the collection plate 41, the front and back sides of the sample collection chamber 411 and the clean water collection chamber 412 are closed to form the sample collection chamber 411 and the clean water collection chamber 412.

[0167] The volume of the clean water collection chamber 412 is larger than that of the sample collection chamber 411 . The clean water collection chamber 412 and the sample collection chamber 411 are respectively located on two sides of the collection plate 41 , and an isolation portion is provided in the middle.

[0168] A sample collecting groove 401 is provided on the top surface of the collecting plate 41 . The sample collecting groove 401 is formed by a depression on the top surface of the collecting plate 41 .

[0169] The bottom wall of the sample collecting groove 401 is further provided with a sampling channel, and the sampling channel is formed by the bottom wall of the sample collecting groove 401 being recessed into the sample collecting cavity 411 .

[0170] The sample outlet channel 415 is also formed by the bottom wall of the sample collection tank 401 being recessed to the bottom of the sample collection chamber 411. The sample outlet channel 415 is located on the isolation portion, and the top of the sample outlet channel 415 is a vertical flow channel extending vertically upward, while the middle area is a curved arc flow channel, and the bottom end is a horizontally extending channel, which extends to the bottom of the sample collection chamber. This curved design of the sample outlet channel 415 can also make the sample fit the inner wall of the sample outlet channel 415, and mix during the flow process to reduce bubbles.

[0171] A sewage outlet 417 is also provided at the bottom end of the sample collection chamber 411 , and the outlet is open toward the bottom end of the collection plate 41 , and excess sample can flow into the toilet from the sewage flow channel 302 .

[0172] The water inlet channel 413 is also formed by the bottom wall of the sample collection tank 401 being recessed into the clean water collection chamber 412, and the water inlet channel 413 is also located on the front side of the collection plate 41. A pipe can be inserted at the top of the water inlet channel 413, and the water inlet channel 413 is connected to the clean water collection member through the pipe, which is similar to the previous embodiment and will not be described in detail.

[0173] The water outlet channel 414 is formed by the bottom of the clean water collection chamber 412 being bent upward and extending to the bottom wall of the sample collection groove 401. The water outlet channel 414 is formed by the depression on the rear side of the collection plate 41 and is also located on the isolation portion. The water outlet channel 414 also has a sample bending shape. During the clean water collection process, it can also reduce the bubbles in the clean water from entering the clean water flow channel of the flow channel plate 3.

[0174] The rear side of the isolation part is also provided with a drainage channel 416, the top of which extends to the bottom wall of the sample collection tank 401 and is connected to the drainage transfer pipe 3014 through a pipe, and the bottom of the drainage channel 416 is connected to the liquid outlet 421 of the inner cover plate 42, and the inner cover plate 42 covers the rear side of the collection plate 41 and is used to adhere to the inner wall of the toilet. The liquid in the detection cavity can flow into the drainage channel 416 from the drainage flow channel 302 and the drainage transfer pipe 3014 in sequence, and then flow into the toilet from the liquid outlet 421.

[0175] The collector also includes two liquid level sensors, which are respectively installed in the clean water collection chamber 412 and the sample collection chamber 411 to sense the liquid levels of the clean water and the sample.

[0176] A sample flow channel may be provided on the flow channel plate 3, and the sample flow channel may be directly connected to the sample collection chamber 411 through the sample transfer tube 3011. In another embodiment, two sample flow channels may be provided on the flow channel plate 3, and the inner ends of the two sample flow channels are respectively connected to two circumferential holes 32, and the outer ends are respectively connected to two pipelines through two sample transfer tubes 3011.

[0177] The two sample flow channels are defined as an upper sample flow channel 341 and a lower sample flow channel 342, and the pipes connected to the two sample flow channels are an upper sample pipe 344 and a lower sample pipe 345. The upper sample flow channel 341 is connected to the upper sample pipe 344 through a sample transfer pipe 3011, and the lower sample flow channel 342 is connected to the lower sample pipe 345 through a sample transfer pipe 3011.

[0178] One end of the lower sample pipe is connected to the lower sample flow channel 342, and the other end is connected to the sample outlet channel 415 of the sample collection chamber 411, and a burette is set in the middle of the lower sample pipe, which is defined as the sample burette 343. One end of the upper sample pipe is connected to the upper sample flow channel 341, and the other end is connected to the top of the sample burette 343. When it is necessary to extract a sample, the rotor 6 needs to be aligned with the circumferential hole 32 connected to the upper sample flow channel 341, and the air in the upper sample flow channel 341, the upper sample pipe and the sample burette 343 is extracted, and a negative pressure is formed in the sample burette 343, and the sample can be dripped from the sample collection chamber 411 into the sample burette 343. Then the rotor 6 is rotated again, so that the transfer channel is aligned with the circumferential hole 32 of the lower sample flow channel 342, and the air in the lower sample flow channel 342 and the lower sample pipe is extracted, and the liquid in the sample burette 343 flows from the lower sample pipe into the lower sample flow channel 342. This arrangement can ensure that the sample drips slowly into the lower sample flow channel 342 without generating bubbles.

[0179] Of course, in another embodiment, an exhaust hole 418 is provided in the sample collection chamber 411, the upper sample flow channel 341 can be connected to the exhaust hole 418, and the lower sample flow channel 342 is connected to the sample outlet channel 415 of the sample collection chamber 411. At this time, the sample collection chamber 411 is similar to the above-mentioned sample burette, and the operation method is the same as above, which will not be repeated.

[0180] The flow channel plate 3 may also be provided with one clean water flow channel, which is directly connected to the clean water collection chamber 412 .

[0181] Of course, two clean water channels are provided on the flow channel plate 3, and the two clean water channels are respectively an upper clean water channel 351 and a lower clean water channel 352 based on the same principle as the above two sample channels. The outer ends of the two clean water channels are respectively connected with the two circumferential holes 32. Similarly, the outer end of the lower clean water flow is connected with the clean water transfer tube 3012, and the clean water transfer tube 3012 is connected with the clean water collection chamber 412 through the upper clean water pipe 354. The lower clean water pipe 355 is also provided with a clean water burette 353. The upper clean water pipe 354 is connected with the clean water burette 353 of the lower clean water pipe 355.

[0182] The upper clean water flow channel 351 is connected to the clean water transfer tube 3012 , and the clean water transfer tube 3012 is connected to the upper end of the clean water burette 353 through the upper clean water pipeline 354 .

[0183] When it is necessary to extract a sample, the rotor 6 needs to be aligned with the circumferential hole 32 connected to the upper clear water flow channel 351, and the air in the upper clear water flow channel 351, the sample clear water pipeline and the clear water burette 353 is extracted to form a negative pressure in the clear water burette, and the clear water can drip from the clear water collection chamber 412 into the clear water burette 353. Then the rotor 6 is rotated again so that the transfer channel is aligned with the circumferential hole 32 of the lower clear water flow channel 352, and the air in the lower clear water flow channel 352 and the lower clear water pipeline is extracted, and the liquid in the clear water burette 353 flows from the lower clear water pipeline into the lower clear water flow channel 352. This arrangement can ensure that water slowly drips into the lower clear water flow channel 352 without generating bubbles.

[0184] The present invention also relates to a control method of a urine tester 100, which includes a reagent kit 2, a collector, a rotary cutting valve and the above-mentioned detection mechanism. The reagent kit 2 is the same as the above-mentioned one, and is provided with a plurality of reagent chambers 21, in which reagents are placed. The collector is also provided with the above-mentioned sample collection chamber 411 and the clean water collection chamber 412. The rotary cutting valve is the same as the above-mentioned one, and includes a microfluidic chip and a rotor 6. The microfluidic chip is also provided with a common flow channel 33, a lower sample flow channel 342, an upper sample flow channel 341, an upper clean water flow channel 351, a lower clean water flow channel 352, a plurality of reagent flow channels 37, a detection flow channel 38, an air flow channel 36 and a transfer channel. The outer end of the lower sample flow channel 342 is connected to the sample collection chamber 411 through a lower sample pipe 345, and the lower sample pipe 345 is provided with a sample burette 343. The outer end of the upper sample flow channel 341 is connected to the top of the sample burette 343. The outer ends of the multiple reagent flow channels 37 are respectively connected to the multiple reagent chambers 21. The outer end of the lower clean water flow channel 352 is connected to the clean water collection chamber 412. The rotor 6 is provided with a transfer channel, which can drive the transfer channel to rotate when the rotor 6 rotates. The inner end 61 of the transfer channel is located at the center of the rotor 6 and is connected to the inner end of the common flow channel 33. The outer end rotates with the rotor 6 and is connected to the inner end of the lower sample flow channel 342, the upper sample flow channel, the upper clean water flow channel 351, the lower clean water flow channel 352, multiple reagent flow channels 37, the detection flow channel 38 and the air flow channel 36. That is to say, each time the rotary cutting valve switches to the lower sample flow channel 342, the upper sample flow channel, the upper clean water flow channel 351, the lower clean water flow channel 352, the multiple reagent flow channels 37, the detection flow channel 38 and the air flow channel 36, the transfer channel is connected with the lower sample flow channel 342, the upper sample flow channel, the upper clean water flow channel 351, the lower clean water flow channel 352, the multiple reagent flow channels 37, the detection flow channel 38 and the air flow channel 36.

[0185] The detection mechanism is consistent with the above, and is provided with a detector and a detection cavity. The detection cavity is connected to the detection channel 38, and the detector is used to detect the sample in the detection cavity.

[0186] like Fig.31 As shown, the control method of the urine test instrument 100 includes the following steps: S1, the rotary cutting valve is switched to the upper sample flow channel 341, the transfer channel is connected to the upper sample flow channel 341, the air in the sample burette 343 is sucked into the transfer channel and the common flow channel 33, the sample burette 343 forms a negative pressure, and the sample in the sample collection chamber 411 enters the sample burette 343. The rotary cutting valve is switched to the upper sample flow channel, the common channel is connected to the upper sample flow channel, the air in the sample burette 343 can be sucked into the common flow channel 33, so that the sample burette 343 forms a negative pressure. After entering the sample burette 343, the sample flows into the bottom end of the sample burette 343 under the action of gravity.

[0187] S2, the rotary cutting valve is switched to the lower sample flow channel 342, the transfer channel is connected to the lower sample flow channel 342, and the sample in the sample burette 343 is drawn into the common flow channel 33 or the transfer channel. At this time, part of the sample enters the transfer channel, and most of it stays in the lower sample flow channel 342, which is convenient for subsequent quantitative extraction.

[0188] S3, the rotary cutting valve is switched to the lower clean water flow channel 352, the transfer channel is connected to the lower clean water flow channel 352, and the water in the clean water collection chamber 412 is pumped into the common flow channel 33 or the transfer channel. Because the transfer channel is connected to the common flow channel 33, if the sample and water entering the transfer channel are large, they may enter the common flow channel 33. The water and sample entering the common flow channel 33 or the transfer channel are mixed.

[0189] S4, discharge the sample and water in the common flow channel 33 from the outer end of the common flow channel 33. The sample and water may flow out from the outer end of the common flow channel 33 or from the detection cavity, and the specific discharge method is not limited.

[0190] S5, the rotary cutting valve is switched to the lower sample flow channel 342, the transfer channel is connected to the lower sample flow channel 342, and the sample in the lower sample flow channel 342 is quantitatively extracted into the common flow channel 33 or the transfer channel. Step S2 draws the sample into the lower sample flow channel 342, and the sample in the lower sample flow channel 342 basically flows to the inner end of the lower sample flow channel 342, so the sample can be quantitatively extracted according to the time of extracting the sample.

[0191] S6, the rotary cutting valve is switched to the reagent flow channel 37, the transfer channel is connected to the reagent flow channel 37, and the reagent in the reagent chamber 21 is quantitatively extracted into the common flow channel 33 or the transfer channel. The reagent flow channel 37 is always connected to the reagent chamber 21, so a part of the reagent in the reagent chamber 21 is located in the reagent flow channel 37 and can be directly quantitatively extracted.

[0192] S7, the rotary cutting valve is switched to the detection channel 38, the transfer channel is connected to the detection channel 38, and the sample and reagent in the common channel 33 or the transfer channel are pushed into the detection cavity.

[0193] S8. The detector detects the sample in the detection chamber.

[0194] The outer end of the transfer channel of the rotor is connected to different flow channels as the rotary cutting valve is switched. For example, when the rotary cutting valve is switched to the reagent flow channel, the outer end of the transfer channel is connected to the reagent flow channel. The same is true when the rotary cutting valve is switched to other flow channels. No further details will be given.

[0195] Furthermore, the urine test instrument 100 includes a first pump and a second pump, the first pump is the peristaltic pump 91 mentioned above, the second pump is the plunger pump 92, the first pump is connected to a first drive pipeline, and the first drive pipeline is connected to the outer end of the common flow channel 33. The second pump is connected to a second drive pipeline, and the second drive pipeline is connected to the outer end of the common flow channel 33.

[0196] In the above steps, in step S1 , the first pump rotates forward to extract the air in the sample burette 343 .

[0197] In step S2 , the first pump rotates forward to draw the sample in the sample burette 343 into the common flow channel 33 .

[0198] In step S3 , the first pump rotates forward to pump the water in the clean water collection chamber 412 into the common flow channel 33 .

[0199] In step S4, the first pump rotates forward to discharge the sample and water in the common flow channel 33 through the outer end of the first driving pipe.

[0200] In step S5 , the second pump rotates forward and quantitatively draws the sample in the lower sample flow channel 342 into the common flow channel 33 .

[0201] In step S6 , the second pump rotates forward and quantitatively draws the reagent in the reagent chamber 21 into the common flow channel 33 .

[0202] In step S7, the second pump is reversed to push the sample and reagent in the common flow channel 33 into the detection chamber.

[0203] That is to say, the first pump has a large stroke and can extract a large amount of clean water or samples, while the second pump is mainly responsible for quantitatively extracting samples and reagents.

[0204] Optionally, the lower clean water flow channel 352 is connected to the clean water collection chamber 412 through a lower clean water pipeline 355, and the lower clean water pipeline 355 is provided with a clean water burette 353; The rotary cutting valve is provided with upper clear water flow, and the upper clear water flow channel 351 is connected with the clear water burette 353; In step S3, the rotary cutting valve is switched to the upper clean water flow channel 351, the transfer channel is connected to the upper clean water flow channel 351, the first pump rotates forward, and the air in the clean water burette 353 is extracted, and the clean water burette 353 forms a negative pressure. After the clean water in the clean water collection chamber 412 enters the clean water burette 353, the rotary cutting valve is switched to the lower clean water flow channel 352, and the first pump rotates forward to pump the water in the clean water burette 353 into the lower clean water flow channel 352.

[0205] Optionally, before step S6, determine whether multiple reagents need to be extracted. If one reagent is needed, perform step S6; if multiple reagents are needed, repeat step S6, and then switch the rotary cutting valve to the detection channel, push the reagents and samples in the transfer channel into the detection channel, and then switch the rotary cutting valve to different reagent channels, extract different reagents into the transfer channel, and then repeat pushing the extracted reagents into the detection channel.

[0206] That is to say, each time the rotary cutting valve is switched to a different reagent flow channel, the reagent is drawn into the transfer channel, and then the rotary cutting valve is switched to the detection flow channel, and the reagent in the transfer channel is pushed into the detection flow channel to react with the sample. Then, after waiting for a few seconds or a preset time, the rotary cutting valve is switched to a different reagent flow channel again, and different reagents are drawn into the detection flow channel to react with the sample. The reaction time or waiting time can be set as required. In some embodiments, the waiting time may not be set.

[0207] Some detection items only need one reagent to complete, while some items need two or more reagents. When the detection items that require multiple reagents are carried out, step S6 needs to be repeated multiple times, and different reagents can be extracted each time.

[0208] It should be understood that whether the test item needs to be diluted, the amount of diluted sample required for the test, and the type and amount of reagents required are all set in the control module, so after the test is started, the control module will determine the process to be carried out based on the test item.

[0209] Optionally, in step S5, after the second pump sucks the sample in the lower sample flow channel 342 into the common flow channel 33, the step further includes: the rotary cutting valve switches to the lower clean water flow channel 352, and the second pump quantitatively extracts water in the lower clean water flow channel 352 into the common flow channel 33 and mixes it with the sample in the common flow channel 33 to dilute the sample. Some test items require mixing the sample with clean water to dilute the sample, so a certain amount of clean water needs to be extracted after extracting the sample.

[0210] In step S6, after the second pump rotates forward to push the reagent in the reagent chamber 21 into the common flow channel 33, the step further includes: the rotary cutting valve is switched to the air flow channel 36, and the second pump rotates forward to absorb air into the transfer channel.

[0211] Optionally, in step S5, the second pump quantitatively draws water from the lower clear water flow channel 352 into the common flow channel 33 and mixes the water with the sample in the common flow channel 33, and then dilutes the sample for 8s-12s. The dilution time is preferably 10s.

[0212] As a preferred solution, the rotary cutting valve is further provided with a mixing chamber 39, which is located on the path of the detection flow channel 38 and communicated with the detection flow channel 38; In step S6, after the second pump quantitatively extracts the reagent in the reagent chamber 21 into the common flow channel 33, the step further includes: the rotary cutting valve is switched to the air flow channel 36, and the second pump rotates forward to quantitatively extract air into the common flow channel 33. Also due to the stroke problem, part of the air needs to be extracted.

[0213] In step S7, the rotary cutting valve is switched to the detection channel 38, and the second pump is reversed to push the sample and reagent in the common channel 33 into the mixing chamber 39; The first pump rotates forward for a preset time, and then reverses for a preset time, so that the reagent and sample flow continuously in the mixing chamber 39 to form a mixed liquid. The peristaltic pump 91 flows the reagent and sample continuously in the mixing chamber 39 and mixes them.

[0214] Then the second pump rotates forward to push the mixed liquid in the mixing chamber 39 into the detection chamber.

[0215] Furthermore, the mixing cavity 39 includes a mixing bend 392 and a mixing chamber 391 which are connected to each other, and the mixing bend 392 is a curved flow channel.

[0216] In step S7, the second pump reverses to push the sample and reagent in the common flow channel 33 into the mixing chamber 39, and then the first pump rotates forward for a preset time and then reverses for a preset time to make the reagent and sample flow continuously in the mixing bend 392 and the mixing chamber 39 to form a mixed liquid.

[0217] Optionally, the rotary cutting valve is in a vertical state and is provided with a mixing chamber 39, the mixing chamber 39 is located on the path of the detection channel 38 and is connected to the detection channel 38, the mixing chamber 39 has an inlet and an outlet, and the inlet is located below the outlet. The flow channel plate 3 is placed in a vertical state, the mixing chamber 39 is located above the common flow channel 33, the inlet of the mixing chamber 39 is at the bottom, and the outlet is at the top, and the outlet refers to an end opening that flows to the detection chamber.

[0218] In step S6, after the second pump quantitatively extracts the reagent in the reagent chamber 21 into the common flow channel 33, the following steps are also included: the rotary cutting valve is switched to the air flow channel 36, and the second pump rotates forward to quantitatively extract air into the common flow channel 33; In step S7, the rotary cutting valve is switched to the detection flow channel 38, the second pump is reversed, and the sample and reagent in the common flow channel 33 are pushed from the inlet into the top of the mixing chamber 39, and the sample and reagent move downward by gravity, and then the first pump is reversed for a preset time, and the reagent and sample are pushed into the top of the mixing chamber 39 again, and then the first pump stops moving, and the sample and reagent move to the bottom of the mixing chamber 39 again by gravity to flow continuously and form a mixed liquid; Finally, the second pump rotates forward to push the mixed liquid in the mixing chamber 39 into the detection chamber.

[0219] Preferably, after step S8, step S9 is further included: the rotary cut valve is switched to the lower clean water flow channel 352, the first pump rotates forward to extract the water in the lower clean water flow channel 352 into the common flow channel 33, and then the rotary cut valve is switched to the detection flow channel 38, the first pump rotates reversely to push the water in the common flow channel 33 into the detection flow channel 38 and the detection chamber and then discharge it. After the detection is completed, the detection chamber is cleaned with clean water.

[0220] Optionally, after step S8, step S9 is further included: the rotary cut valve is switched to the lower clean water flow channel 352, the first pump rotates forward to extract the water in the lower clean water flow channel 352 into the common flow channel 33, and then the rotary cut valve is switched to the air flow channel 36, the first pump rotates forward to extract air and then discharges the water in the common flow channel 33 from the first drive pipe. After the detection is completed, the common flow channel 33 and the first drive pipe are cleaned with clean water, the first drive pipe is connected to the collector, and the waste liquid can be discharged from the collector into the toilet.

[0221] Optionally, after step S8, step S9 is further included: the rotary cutting valve is switched to the lower clean water flow channel 352 for communication, the first pump rotates forward to extract the water in the lower clean water flow channel 352 into the common flow channel 33, and then the rotary cutting valve is switched to the lower sample flow channel 342 for communication, the first pump rotates reversely and pushes the water in the common flow channel 33 into the lower sample pipe 345, and discharges it from the sample collection chamber 411. After the detection is completed, clean water is extracted to clean the lower sample flow channel 342, the lower sample pipe 345 and the sample collection chamber 411, and the waste liquid is discharged from the sample collection chamber 411.

[0222] After the urine tester 100 is used for several months or after a preset number of operations, the reagents in the reagent box 2 are used up and the reagent box 2 needs to be replaced. When replacing the reagent box 2, the reagent flow channel 37 needs to be thoroughly cleaned, and the washing flow channel of the flow channel plate 3 needs to be used.

[0223] The urine testing instrument 100 further includes a washing liquid and a washing chamber for containing the washing liquid.

[0224] Replacing the kit includes the following steps: S01: The rotary cutting valve switches to the lower clean water flow channel 352, the first pump rotates forward and quantitatively draws clean water into the common flow channel 33, and then the rotary cutting valve switches to the multiple reagent flow channels 37 in turn, the first pump reverses in turn, and pushes the water in the common flow channel 33 into the multiple reagent flow channels 37 in turn, and flows from the multiple reagent flow channels 37 into the multiple reagent chambers 21.

[0225] S02: Replace the reagent kit 2.

[0226] After cleaning the reagent flow channel 37 with clean water, push the waste liquid into the reagent chamber 21 of the reagent kit 2, and then replace the reagent kit 2.

[0227] Optionally, before step S01, the method further includes the following steps: The rotary cut valve is switched to the lower clean water flow channel 352, and the first pump rotates forward to draw clean water into the common flow channel 33. Then the rotary cut valve is switched to the air flow channel 36, and the first pump rotates forward to draw air into the common flow channel 33, and the clean water in the common flow channel 33 is discharged from the first driving pipeline. The rotary cutting valve switches to the lower clean water flow channel 352 again, the first pump rotates forward to draw clean water into the common flow channel 33, and then the rotary cutting valve switches to the lower sample flow channel 342, the first pump rotates backward to push the clean water in the common flow channel 33 into the lower sample pipe 345, and is discharged from the sample collection chamber 411; The rotary cut valve is switched to the lower clean water channel 352 again, and the first pump rotates forward to draw clean water into the common channel 33; then the rotary cut valve is switched to the detection channel 38, and the first pump reverses to push the clean water in the common channel 33 into the detection channel 38 and discharges it from the detection chamber.

[0228] First, clean water is drawn to wash the common flow channel 33 , the sample collection cavity 411 and the detection cavity, and then the reagent flow channel 37 is cleaned.

[0229] Optionally, before step S01, the method further includes the following steps: The rotary cut valve is switched to the lower clean water flow channel 352, the first pump rotates forward and quantitatively draws clean water into the common flow channel 33, and then switches to the washing flow channel, the second pump rotates forward and draws the washing liquid in the washing chamber into the common flow channel 33; Then the rotary cutting valve switches to the detection flow channel 38, the first pump reverses and sucks the clean water and washing liquid in the common flow channel 33 into the mixing chamber 39, and then the first pump rotates forward for a preset time, and then reverses for a preset time, so that the clean water and washing liquid in the mixing chamber 39 flow continuously and mix into diluted washing liquid, and then the first pump rotates forward and sucks the diluted washing liquid in the mixing chamber 39 into the common flow channel 33; The rotary cutting valve switches to the multiple reagent flow channels 37 in sequence, and the first pump reverses and pushes the diluted washing liquid in the common flow channel 33 into the multiple reagent chambers 21 in sequence.

[0230] After the common flow channel 33, the sample collection chamber 411 and the detection chamber are cleaned with clean water, the washing liquid and clean water are repeatedly mixed to form a diluted washing liquid. After the reagent flow channel 37 is cleaned with the diluted washing liquid, step S01 is performed.

[0231] After the urine tester 100 runs for a preset period, for example, after a few months, it is necessary to thoroughly clean the internal structure, which includes the following steps: S001: The rotary cut valve and the lower clean water flow channel 352, the first pump rotates forward and quantitatively draws clean water into the common flow channel 33, then the rotary cut valve and the washing flow channel, the second pump rotates forward and draws the washing liquid in the washing chamber into the common flow channel 33; S002: Then the rotary cutting valve switches to the detection flow channel 38, the first pump reverses and sucks the clean water and washing liquid in the common flow channel 33 into the mixing chamber 39, then the first pump rotates forward for a preset time, and then reverses for a preset time, so that the clean water and washing liquid in the mixing chamber 39 flow continuously and mix into diluted washing liquid, and then the first pump rotates forward and sucks the diluted washing liquid in the mixing chamber 39 into the common flow channel 33. Thorough cleaning requires diluted washing liquid, so water and washing liquid are mixed first.

[0232] S003: the rotary cutting valve is switched to the lower sample flow channel 342, and the first pump is reversed to push the dilution washing liquid in the common flow channel 33 from the lower sample pipe 345 into the sample collection chamber 411; S004: repeat S001 and S002, then the first pump reverses to push the diluted washing liquid in the common flow channel 33 into the detection chamber; S005: After waiting for a preset time, the rotary cutting valve switches to the lower clean water channel 352, the first pump rotates forward and quantitatively draws clean water into the common channel 33, and then pushes the clean water in the common channel 33 into the detection chamber, the first drive pipe and the clean water collection chamber and discharges it.

[0233] The diluted washing liquid is soaked in the sample collection cavity 411 and the detection cavity for a preset time and then discharged.

[0234] Optionally, step S005 includes: S0051: the rotary cut valve is switched to the lower clean water flow channel 352, the first pump rotates forward and quantitatively draws clean water into the common flow channel 33, the rotary cut valve is switched to the detection flow channel 38, the first pump rotates reversely to push the clean water in the common flow channel 33 into the detection chamber and discharge it; S0052: the rotary cut valve is switched to the lower clean water flow channel 352, the first pump rotates forward and quantitatively draws clean water into the common flow channel 33, the rotary cut valve is switched to the air flow channel 36, the first pump rotates forward to draw external air into the common flow channel 33, and then the clean water in the common flow channel 33 is discharged from the first drive pipe; S0053: the rotary cutting valve is switched to the lower clean water flow channel 352, and after the first pump rotates forward and quantitatively draws clean water into the common flow channel 33, the rotary cutting valve is switched to the lower sample flow channel 342, and the first pump reverses to push the clean water in the common flow channel 33 into the sample collection chamber 411 and discharges it.

[0235] First, water is pumped into the common flow channel 33, and then the diluted washing liquid is pushed out from the sample collection chamber 411, the detection chamber and the first driving pipeline by water.

[0236] Optionally, before step S001, the method further includes the following steps: The rotary cut valve is switched to the lower clean water flow channel 352, and the first pump rotates forward to draw clean water into the common flow channel 33. Then the rotary cut valve is switched to the air flow channel 36, and the first pump rotates forward to draw air into the common flow channel 33, and the clean water in the common flow channel 33 is discharged from the first driving pipeline. The rotary cutting valve is switched to the lower clean water flow channel 352, the first pump rotates forward to extract clean water into the common flow channel 33, and then the rotary cutting valve is switched to the lower sample flow channel 342, the first pump rotates backward to push the clean water in the common flow channel 33 into the lower sample pipe 345, and discharged from the sample collection chamber 411; The rotary cut valve switches to the lower clean water channel 352, the first pump rotates forward to draw clean water into the common channel 33, and then the rotary cut valve switches to the detection channel 38, the first pump reverses to push the clean water in the common channel 33 into the detection channel 38, and discharges it from the detection chamber.

[0237] The urine tester also includes a control module. After step S8, that is, after the test is completed, the detector sends the test result to the control module. If the test result is abnormal, the control module restarts steps S1 to S8; if the test result is normal, the next test item is performed.

[0238] Furthermore, the urine test instrument further comprises a warning light, and the warning light is connected to the control module; After step S8, if the detection results are abnormal for two consecutive times, the control module controls the signal light to warn.

[0239] In addition, the control module is also connected to the liquid level sensors in the clean water collection chamber and the sample collection chamber.

[0240] Before step S1 , the liquid level sensor in the sample collection chamber can send a liquid level signal in the sample collection chamber to the control module. If the liquid level meets the requirement, S1 is performed; otherwise, the process ends.

[0241] Step S3 is the same as the step, the control module receives the liquid level of the liquid level sensor in the clean water collection chamber to determine whether the clean water level meets the requirements, if it meets the requirements, proceed to the next step. If it does not meet the requirements, the process ends.

[0242] In addition, the urine test instrument also includes a fingerprint module or a sensing module, which is connected to the control module. The operator can control the urine test instrument to start by fingerprint, or the sensing module can sense the operator's action and transmit the signal to the control module to start the operation.

[0243] The present invention also relates to a method for using a microfluidic chip, wherein the flow channel plate 3 is provided with the above-mentioned sample flow channel, reagent flow channel 37, detection flow channel 38, and common flow channel 33, and the common flow channel 33 is operably connected with the sample flow channel, reagent flow channel 37, and detection flow channel 38. The mixing chamber 39 is located on the path of the detection flow channel 38 and has an outlet and an inlet, and the mixing chamber 39 includes a mixing bend 392 and a mixing chamber 391, and the mixing bend 392 is a curved flow channel and is located on the path of the detection flow channel 38. The width dimension of the mixing chamber 391 is greater than the diameter dimension of the detection flow channel 38.

[0244] The method for using the microfluidic chip comprises the following steps: S11, driving the sample in the sample flow channel into the common flow channel 33; S12, driving the reagent in the reagent flow channel 37 into the common flow channel 33; S13, drive the sample and reagent in the common flow channel 33 to enter the entrance of the mixing chamber 39 from the detection flow channel 38, enter the mixing bend 392 from the entrance of the mixing chamber 39, and then flow into the mixing chamber 391 to form a mixed liquid, and then drive the mixed liquid to flow into the detection flow channel 38 again from the outlet of the mixing chamber 39, and flow out of the detection flow channel 38.

[0245] The sample flow channel is communicated with the sample collection chamber, the reagent flow channel 37 is communicated with the reagent chamber 21 for containing the reagent, and the detection flow channel 38 is communicated with the detection chamber.

[0246] The common flow channel 33 can be connected to the sample flow channel, the reagent flow channel 37 or the detection flow channel 38 through a three-way valve or other valves. The air in the common flow channel 33 can be extracted by a peristaltic pump 91 or a plunger pump 92 to form a negative pressure, and then the sample in the sample flow channel or the reagent in the reagent flow channel 37 can be driven into the common flow channel 33.

[0247] Optionally, the mixing chamber 391 is located above the mixing bend 392, and the inlet of the mixing chamber 39 is below the outlet of the mixing chamber 39; In step S13, the sample and reagent in the common flow channel 33 are driven to enter the entrance of the mixing chamber 39 from the detection flow channel 38, enter the mixing bend 392 from the entrance of the mixing chamber 39, and then flow into the mixing chamber 391, and then the mixed liquid flows back from the mixing chamber 39 into the mixing bend 392, and the sample and reagent flow back by gravity. Then the mixed liquid is driven to flow from the mixing bend 392 into the mixing chamber 39, and finally the mixed liquid is driven to enter the detection flow channel 38 again from the mixing chamber 39, and then flows out from the detection flow channel 38.

[0248] Optionally, the mixing chamber 391 is located above the mixing bend 392; in step S3, the sample and reagent in the common flow channel 33 are driven to enter the entrance of the mixing chamber 39 from the detection flow channel 38, enter the mixing bend 392 from the entrance of the mixing chamber 39, and then flow into the mixing chamber 391, and then the mixed liquid flows back from the mixing chamber 391 into the mixing bend 392, stays for 1s-2s, and then drives the mixed liquid to flow from the mixing bend 392 into the mixing chamber 391, and finally drives the mixed liquid from the mixing chamber 391 to enter the detection flow channel 38 again, and flows out from the detection flow channel 38.

[0249] During the mixing process of samples and reagents, staying for 1s-2s can improve the mixing effect.

[0250] As a preferred embodiment, the mixing cavity 39 includes a plurality of mixing bends 392 and a plurality of mixing chambers 391, and the plurality of mixing bends 392 and the plurality of mixing chambers 391 are arranged at intervals and communicated with each other; In step S13 , the sample and reagent in the common flow channel 33 are driven to enter the entrance of the mixing chamber 39 from the detection flow channel 38 , enter the multiple mixing bends 392 and the multiple mixing chambers 39 from the entrance of the mixing chamber 39 , and finally the mixed liquid is driven to flow out of the detection flow channel 38 .

[0251] Further, the mixing bend 392 is an S-shaped bend and includes at least two horizontal flow channels and at least one turning flow channel 394, and any turning flow channel 394 is located at both ends of the two horizontal flow channels; The horizontal flow channel extends in the horizontal direction. The turning flow channel 394 includes a curved inner arc 395 and an outer arc 396 . The inner arc 395 and the outer arc 396 are both arc-shaped, and the inner diameter of the inner arc 395 is smaller than the inner diameter of the outer arc 396 .

[0252] Optionally, the inner wall of the mixing chamber 391 includes a first curved surface 397 and a second curved surface 398 opposite to the first curved surface 397 , and the first curved surface 397 and the second curved surface 398 are curved surfaces protruding toward the outside.

[0253] Optionally, each mixing chamber 391 is connected to the horizontal flow channels of the two mixing bends 392, and the two horizontal flow channels are radially symmetrically arranged at the top and bottom of the mixing chamber 391; In step S13, the sample and reagent in the common flow channel 33 are driven from the detection flow channel 38 into the entrance of the mixing chamber 39, enter a mixing bend 392 from the entrance of the mixing chamber 39, and then flow into the mixing chamber 391, and then flow from the mixing chamber 39 into another mixing bend 392 to form a mixed liquid. After staying for 1s-2s, the mixed liquid flows back into the mixing chamber 391, and the mixed liquid is driven to be discharged from the mixing bend 392.

[0254] In one embodiment, each mixing chamber has an inlet and an outlet, respectively, the inlet and outlet of each mixing chamber are respectively connected to the adjacent mixing bend, and the inlet of each mixing chamber is located above the outlet; In step S13, after the sample and reagent enter the mixing chamber, they flow from one of the mixing bends through an adjacent one of the inlets into the mixing chamber, and then flow from the outlet into another mixing bend. The sample and reagent flow continuously in multiple mixing chambers and multiple mixing bends to form the mixed liquid.

[0255] The microfluidic chip can be placed in a vertical direction, that is, when samples and reagents flow from bottom to top, there is no need to consider the influence of gravity, so there will be no residue problems in the mixing chamber.

[0256] Optionally, the flow channel plate 3 is further provided with a clean water flow channel, and the common flow channel 33 can be connected to the clean water flow channel through the transfer channel of the rotor 6; After step S13, step S14 is also included: driving the water in the clean water channel into the common channel 33, and then driving the water in the common channel 33 into the detection channel 38, and then entering the mixing chamber 39 from the entrance of the mixing chamber 39, and then entering the detection channel 38 again from the outlet of the mixing chamber 39, and being discharged from the detection channel 38.

[0257] As a preferred embodiment, the flow channel plate 3 is also provided with a central hole 31 and a plurality of circumferential holes 32 arranged around the central hole 31. The central hole 31 passes through the flow channel plate 3 and is connected to the inner end of the common flow channel 33. The plurality of circumferential holes 32 are arranged along the circumferential direction formed with the central hole 31 as the center. The inner end of the common flow channel 33 is connected to the central hole 31, and the outer end is connected to the pump through a driving pipe. The inner end of the sample flow channel is connected to a circumferential hole 32. The inner end of the reagent flow channel 37 is connected to a circumferential hole 32. The inner end of the detection flow channel 38 is connected to a circumferential hole 32; The central hole 31 is communicated with the inner end of a transfer channel of a rotor 6 , and the outer end of the transfer channel can be communicated with any circumferential hole 32 as the rotor 6 rotates.

[0258] In step S11, the rotor 6 rotates, the transfer channel is connected to the sample flow channel through one of the circumferential holes 32, and the pump draws out the air in the common flow channel 33 and the sample flow channel, and draws the sample in the sample flow channel into the common flow channel 33; In step S12, the rotor 6 rotates, the transfer channel is connected to the reagent flow channel 37 through one of the circumferential holes 32, the pump extracts the air in the common flow channel 33 and the reagent flow channel 37, and draws the sample in the reagent flow channel 37 into the common flow channel 33; In step S13, the rotor 6 rotates, and the transfer channel is connected to the detection channel 38 through one of the circumferential holes 32; the pump drives the sample and reagent in the common channel 33 to enter the entrance of the mixing chamber 39 from the detection channel 38, enter the mixing bend 392 from the entrance of the mixing chamber 39, and then flow into the mixing chamber 391 to form a mixed liquid, and then drive the mixed liquid to flow from the outlet of the mixing chamber 39 into the detection channel 38 again, and flow out of the detection channel 38.

[0259] As another preferred solution, the flow channel plate 3 is provided with two sample flow channels, namely, an upper sample flow channel 341 and a lower sample flow channel 342. The inner end of the lower sample flow channel 342 and the inner end of the upper sample flow channel 341 are respectively connected to the two circumferential holes 32. The lower sample flow is connected to the sample collection cavity containing the sample through the lower sample pipe, and the above-mentioned sample burette is provided on the lower sample pipe. The upper sample flow channel 341 is connected to the sample burette through the upper sample pipe; In step S11, the rotor 6 rotates to align the outer end 62 of the transfer channel with the circumferential hole 32 connected to the upper sample flow channel 341, and the pump cooperates with the driving pipeline to extract the air in the common flow channel 33, the transfer channel, the upper sample flow channel 341 and the sample burette.

[0260] Then the rotor 6 rotates and aligns the outer end 62 of the transfer channel with the circumferential hole 32 connected to the lower sample flow channel 342 , and draws the sample in the sample collection chamber from the lower sample flow channel 342 to the common flow channel 33 .

[0261] Providing a sample burette can reduce the amount of bubbles in the sample collection cavity that flow into the sample flow channel.

[0262] Optionally, the flow channel plate 3 is further provided with a clean water flow channel, and the clean water flow channel is connected to a circumferential hole 32 .

[0263] After step S13, step S14 is also included: the rotor 6 rotates, the transfer channel is connected to the clean water channel through one of the circumferential holes 32, the pump drives the water in the clean water channel into the common channel 33, and then drives the water in the common channel 33 into the detection channel 38, and then enters the mixing chamber 39 from the entrance of the mixing chamber 39, and then enters the detection channel 38 again from the outlet of the mixing chamber 39, and is discharged from the detection channel 38.

[0264] Preferably, the flow channel plate 3 is provided with an upper clean water flow channel 351 and a lower clean water flow channel 352, and the inner ends of the upper clean water flow channel 351 and the lower clean water flow channel 352 are respectively connected to the two circumferential holes 32. The lower clean water flows through the lower clean water pipe and is connected to the clean water collection chamber containing water, and a clean water burette is provided on the lower clean water pipe. The upper clean water flow channel 351 is connected to the clean water burette through the upper clean water pipe.

[0265] After step S13, step S14 is also included: the rotor 6 rotates to align the outer end 62 of the transfer channel with the circumferential hole 32 connected to the upper clear water flow channel 351, and the pump pumps the air in the common flow channel 33, the transfer channel, the upper clear water flow channel 351 and the clear water burette to the outside; Then the rotor 6 rotates to align the outer end 62 of the transfer channel with the circumferential hole 32 connected to the lower clean water flow channel 352, and the clean water in the clean water collection chamber is pumped from the lower clean water flow channel 352 to the common flow channel 33. The rotor 6 rotates to align the common flow channel 33 with the circumferential hole 32 of the detection flow channel 38, and the water in the common flow channel 33 is detected in turn by the detection flow channel 38 and discharged from the detection flow channel 38.

[0266] Optionally, the flow channel plate 3 is further provided with an air flow channel 36 , and the inner end of the air flow channel 36 is communicated with another circumferential hole 32 .

[0267] In step S13, the rotor 6 rotates, and the transfer channel is connected to the detection channel 38 through one of the circumferential holes 32; the rotor 6 rotates so that the transfer channel is aligned with the circumferential hole 32 connected to the air, so that the common channel 33 is connected to the air channel 36, and the pump draws air from the air channel 36 into the common channel 33.

[0268] Then the rotor 6 rotates, and the transfer channel is connected to the detection channel 38 through one of the circumferential holes 32; the pump drives the sample and reagent in the common channel 33 to enter the entrance of the mixing chamber 39 from the detection channel 38, enter the mixing bend 392 from the entrance of the mixing chamber 39, and then flow into the mixing chamber 391 to form a mixed liquid, and then drive the mixed liquid to flow into the detection channel 38 again from the outlet of the mixing chamber 39, and flow out of the detection channel 38.

[0269] The flow channel plate 3 of the present invention can also be adapted to other urine test instruments 100 , and equipped with the above-mentioned pump or rotor 6 , so as to mix the sample and the reagent in the flow channel plate 3 , reduce bubbles, and increase the accuracy of the test.

[0270] Preferred embodiments of the present invention have been described above in detail, but it should be understood that aspects of the embodiments can be modified, if necessary, to employ aspects, features and concepts of the various patents, applications and publications to provide further embodiments.

[0271] These and other changes can be made to the embodiments in light of the above detailed description.In general, in the claims, the terms used should not be considered limited to the specific embodiments disclosed in the specification and the claims, but should be construed to include all possible embodiments along with the full scope of equivalents to which these claims are entitled.

[0272] Those skilled in the art will appreciate that the above-mentioned embodiments are specific examples for implementing the present invention, and in actual applications, various changes may be made thereto in form and detail without departing from the spirit and scope of the present invention.

Claims

1. A method for using a microfluidic chip, characterized in that: The microfluidic chip is provided with: Sample flow channel; Reagent flow channel; Detect flow channel; a common flow channel, the common flow channel being operably connected to the sample flow channel, the reagent flow channel and the detection flow channel; A mixing chamber, the mixing chamber is located on the path of the detection flow channel and has an outlet and an inlet, and the mixing chamber includes: a mixing bend, which is a curved flow channel and is located on the path of the detection flow channel; and At least one mixing chamber, wherein the width of the mixing chamber is greater than the diameter of the detection channel; The method for using the microfluidic chip comprises the steps of: S11, driving the sample in the sample flow channel into the common flow channel; S12, driving the reagent in the reagent flow channel into the common flow channel; S13, drive the sample and reagent in the common flow channel to enter the inlet of the mixing chamber from the detection flow channel, enter the mixing bend from the inlet of the mixing chamber, and then flow into the mixing chamber to form a mixed liquid, and then drive the mixed liquid to flow into the detection flow channel again from the outlet of the mixing chamber, and flow out of the detection flow channel.

2. The method for using the microfluidic chip according to claim 1, characterized in that: The mixing chamber is located above the mixing bend, and the inlet of the mixing chamber is below the outlet of the mixing chamber; In step S13, the sample and reagent in the common flow channel are driven to enter the entrance of the mixing chamber from the detection flow channel, enter the mixing bend from the entrance of the mixing chamber, and then flow into the mixing chamber. Then the mixed liquid flows back from the mixing chamber into the mixing bend, and then the mixed liquid is driven to flow from the mixing bend into the mixing chamber. Finally, the mixed liquid is driven to enter the detection flow channel from the mixing chamber again and flow out from the detection flow channel.

3. The method for using the microfluidic chip according to claim 1, characterized in that: The mixing chamber is located above the mixing curve; In step S13, the sample and reagent in the common flow channel are driven to enter the entrance of the mixing chamber from the detection flow channel, enter the mixing curve from the entrance of the mixing chamber, and then flow into the mixing chamber. Then, the mixed liquid flows back from the mixing chamber into the mixing curve. After staying for a preset period of time, the mixed liquid is driven to flow from the mixing curve into the mixing chamber. Finally, the mixed liquid is driven from the mixing chamber to enter the detection flow channel again and flow out of the detection flow channel.

4. The method for using the microfluidic chip according to claim 1, characterized in that: The mixing cavity comprises a plurality of mixing bends and a plurality of mixing chambers, wherein the plurality of mixing bends and the plurality of mixing chambers are arranged at intervals and communicated with each other; In step S13, the sample and reagent in the common flow channel are driven to enter the inlet of the mixing chamber from the detection flow channel, enter the multiple mixing bends and multiple mixing chambers from the inlet of the mixing chamber, and finally drive the mixed liquid to flow out of the detection flow channel.

5. The method for using the microfluidic chip according to claim 4, characterized in that: Each of the mixing chambers has an inlet and an outlet, respectively. The inlet and outlet of each mixing chamber are respectively connected to the adjacent mixing bends, and the inlet of each mixing chamber is located above the outlet. In step S13, after the sample and reagent enter the mixing chamber, they flow from one of the mixing bends through an adjacent one of the inlets into the mixing chamber, and then flow from the outlet into another mixing bend. The sample and reagent flow continuously in multiple mixing chambers and multiple mixing bends to form the mixed liquid.

6. The method for using the microfluidic chip according to claim 4, characterized in that: The mixing bend is an S-shaped bend and includes at least two horizontal flow channels and at least one turning flow channel, and any one of the turning flow channels is located at both ends of the two horizontal flow channels; The horizontal flow channel extends in a horizontal direction, and the turning flow channel includes a curved inner arc and an outer arc. Both the inner arc and the outer arc are arc-shaped, and the inner diameter of the inner arc is smaller than the inner diameter of the outer arc.

7. The method for using the microfluidic chip according to claim 4, characterized in that: The inner wall of the mixing chamber includes a first curved surface and a second curved surface opposite to the first curved surface, and the first curved surface and the second curved surface are curved surfaces protruding toward the outside.

8. The method for using the microfluidic chip according to claim 6, characterized in that: Each of the mixing chambers is connected to the horizontal flow channels of the two mixing bends, and the two horizontal flow channels are radially symmetrically arranged at the top and bottom of the mixing chamber; In step S13, the sample and reagent in the common flow channel are driven to enter the entrance of the mixing chamber from the detection flow channel, enter one of the mixing bends from the entrance of the mixing chamber, and then flow into the mixing chamber, and then flow from the mixing chamber into another mixing bend to form a mixed liquid. After staying for a preset period of time, the mixed liquid flows back into the mixing chamber, and the mixed liquid is driven to be discharged from the mixing bend.

9. The method for using the microfluidic chip according to claim 1, characterized in that: The microfluidic chip is also provided with a clean water flow channel; The common flow channel is operably connected to the clean water flow channel; After step S13, step S14 is also included: driving the water in the clean water channel into the common channel, and then driving the water in the common channel into the detection channel, and then entering the mixing chamber from the inlet of the mixing chamber, and then entering the detection channel again from the outlet of the mixing chamber, and being discharged from the detection channel.

10. The method for using the microfluidic chip according to claim 1, characterized in that: The microfluidic chip comprises a flow channel plate and a cover plate covering the front side of the flow channel plate; The sample flow channel, the reagent flow channel, the detection flow channel, the common flow channel and the mixing chamber are formed by the depression of the front side surface of the flow channel plate; The flow channel plate is provided with: A central hole, the central hole passes through the flow channel plate and is connected to the inner end of the common flow channel; A plurality of circumferential holes, wherein the plurality of circumferential holes are arranged along a circumference formed with the central hole as the center; The inner end of the common flow channel is in communication with the central hole, and the outer end of the common flow channel is connected to a pump via a driving pipeline; The inner end of the sample flow channel is in communication with one of the circumferential holes; The inner end of the reagent flow channel is in communication with one of the circumferential holes; The inner end of the detection flow channel is connected to one of the circumferential holes; The central hole is connected to a transfer channel of a rotor, and the outer end of the transfer channel can be connected to any one of the circumferential holes as the rotor rotates; Step S11, the rotor rotates, the transfer channel is connected to the sample flow channel through one of the circumferential holes, the pump draws out the air in the common flow channel and the sample flow channel, and draws the sample in the sample flow channel into the common flow channel; Step S12, the rotor rotates, the transfer channel is connected to the reagent flow channel through one of the circumferential holes, the pump extracts the air in the common flow channel and the reagent flow channel, and draws the sample in the reagent flow channel into the common flow channel; In step S13, the rotor rotates, and the transfer channel is connected to the detection channel through one of the circumferential holes; the pump drives the sample and reagent in the common channel to enter the inlet of the mixing chamber from the detection channel, and then enter the mixing bend from the inlet of the mixing chamber and flow into the mixing chamber to form a mixed liquid, and then drives the mixed liquid to flow into the detection channel again from the outlet of the mixing chamber and flow out of the detection channel.

11. The method for using the microfluidic chip according to claim 10, characterized in that: The flow channel plate is provided with two sample flow channels, namely an upper sample flow channel and a lower sample flow channel; The inner end of the lower sample flow channel and the inner end of the upper sample flow channel are respectively connected to the two circumferential holes; The lower sample flow is connected to the collection chamber containing the sample through the lower sample pipe, and a burette is provided on the lower sample pipe; The upper sample flow channel is connected to the burette through an upper sample pipe; In step S11, the rotor rotates to align the outer end of the transfer channel with the circumferential hole connected to the upper sample flow channel, and the pump drives the driving pipeline to extract the air in the common flow channel, the transfer channel, the upper sample flow channel and the burette; Then the rotor rotates, and aligns the outer end of the transfer channel with the circumferential hole connected to the lower sample flow channel, and draws the sample in the collection chamber from the lower sample flow channel to the common flow channel.

12. The method for using the microfluidic chip according to claim 10, characterized in that: The flow channel plate is also provided with a clean water flow channel, and the clean water flow channel is connected to one of the circumferential holes; After step S13, step S14 is also included: the rotor rotates, the transfer channel is connected with the clean water flow channel through one of the circumferential holes, the pump drives the water in the clean water flow channel to enter the common flow channel, and then drives the water in the common flow channel to enter the detection flow channel, and then enters the mixing chamber from the inlet of the mixing chamber, and then enters the detection flow channel again from the outlet of the mixing chamber, and is discharged from the detection flow channel.

13. The method for using the microfluidic chip according to claim 10, characterized in that: The flow channel plate is provided with an upper clean water flow channel and a lower clean water flow channel, and the inner end of the upper clean water flow channel and the inner end of the lower clean water flow channel are respectively connected to the two circumferential holes; The lower clean water flows through a lower clean water pipe and is connected to a water collection chamber, and a burette is provided on the lower clean water pipe; The upper clear water flow channel is connected to the burette through an upper clear water pipeline; After step S13, step S14 is also included: the rotor rotates to align the outer end of the transfer channel with the circumferential hole connected to the upper clear water flow channel, and the pump pumps the air in the common flow channel, the transfer channel, the upper clear water flow channel and the burette to the outside; Then the rotor rotates to align the outer end of the transfer channel with the circumferential hole connected to the lower clean water flow channel, and the clean water in the collection chamber is pumped from the lower clean water flow channel to the common flow channel. The rotor rotates to align the common flow channel with the circumferential hole of the detection flow channel, and the water in the common flow channel is passed through the detection flow channel in turn and discharged from the detection flow channel.

14. The method for using the microfluidic chip according to claim 10, characterized in that: The flow channel plate is also provided with an air flow channel, the inner end of which is connected to another circumferential hole; Step S13, the rotor rotates, and the transfer channel is connected to the detection channel through one of the circumferential holes; the rotor rotates so that the transfer channel is aligned with the circumferential hole connected to the air, so that the common channel is connected to the air channel, and the pump draws air from the air channel into the common channel; Then the rotor rotates, and the transfer channel is connected to the detection flow channel through one of the circumferential holes; the pump drives the sample and reagent in the common flow channel to enter the inlet of the mixing chamber from the detection flow channel, enter the mixing bend from the inlet of the mixing chamber, and then flow into the mixing chamber to form a mixed liquid, and then drives the mixed liquid to flow into the detection flow channel again from the outlet of the mixing chamber, and then flow out of the detection flow channel.

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