Organic fertilizer prepared from cockroach body waste and preparation method of organic fertilizer

Abstract

The invention belongs to the technical field of fertilizers, and particularly provides an organic fertilizer using cockroach body waste and a preparation method thereof, the organic fertilizer comprises the following raw materials by weight: 60-80 parts of a cockroach-based fertilizer, 15-30 parts of a phosphate fertilizer, 10-25 parts of a potash fertilizer, 10-20 parts of urea, 3-5 parts of alginate oligosaccharide, 2-5 parts of a synergist, and 1-2 parts of trace elements. According to the organic fertilizer, the degradation rate of cockroach body waste is high, the cockroach body waste is rich in amino acid after enzymolysis and fermentation, the production process is simple, the resource utilization rate and the crop quality can be remarkably improved, and the organic fertilizer has social and economic benefits.

Description

Technical Field

[0001] The invention belongs to the technical field of fertilizers, and in particular relates to an organic fertilizer made from cockroach body waste and a preparation method thereof. Background Art

[0002] With the continuous development of global agricultural production, the decline in soil quality and the uneven quality of crops have become urgent problems to be solved. Fertilizer is the cornerstone of agricultural production and is essential for the growth and development of crops. Although traditional fertilizer use methods, such as chemical fertilizers, can quickly increase crop yields, their long-term use has led to increasingly serious problems such as soil degradation, heavy metal accumulation, and ecological environmental pollution.

[0003] In recent years, research on the use of insect waste as organic fertilizer has gradually attracted attention. Cockroaches, as a highly adaptable and rapidly reproducing organism, can effectively consume organic waste, especially food waste. During the digestion process, cockroaches can not only degrade organic matter, but also convert it into nutrient-rich feces, which are considered to have good fertilizer properties. However, the current utilization of cockroach waste is still relatively limited, and the focus is mainly on the research of using it as feed. In the prior art, there is no method of using cockroach waste as a high-protein substance to obtain organic fertilizer using microbial fermentation technology. During the fermentation process, there may often be problems such as slow and unstable protein hydrolysis and low hydrolyzed amino acid content, which leads to a waste of resources.

[0004] Chinese patent CN 113307683 A provides a processing method for converting garbage into organic fertilizer. The method uses urban domestic garbage as raw material, firstly performs a first crushing treatment through a flash blasting device to obtain crushed material I; then, earthworms and cockroaches are added to crushed material I, cultured for 5-7 days, and then a second crushing treatment is performed through a flash blasting device to obtain crushed material II; then, a composite bacterial agent is introduced into crushed material II, fermented, and a fermentation product is obtained; finally, a treatment agent is added to the fermentation product, stirred, magnetically separated, and dried. By this method, garbage and cockroaches can be converted into organic fertilizer together. However, this invention does not use cockroach body waste, and requires specific flash blasting equipment to complete the crushing. This process involves heating and pressurizing operations, and the process is complicated. At the same time, this invention does not perform enzymolysis, so that the amino acid content produced by fermentation is relatively low.

[0005] Therefore, there is an urgent need for an organic fertilizer made from cockroach waste, which contains rich amino acids after enzymatic fermentation and can improve resource utilization and crop quality. Summary of the invention

[0006] In view of the existing technical problems, the purpose of the present invention is to provide an organic fertilizer made of cockroach body waste and a preparation method thereof. The cockroach body waste in the organic fertilizer of the present invention has a high degradation rate, contains rich amino acids after enzymatic hydrolysis and fermentation, has a simple production process, has good stability, can significantly improve resource utilization and the quality of crops, and has both social and economic benefits.

[0007] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0008] On the one hand, the present invention provides an organic fertilizer made from cockroach waste, which comprises the following raw materials by weight: 60-80 parts of cockroach-based fertilizer, 15-30 parts of phosphate fertilizer, 10-25 parts of potash fertilizer, 10-20 parts of urea, 3-5 parts of brown algae oligosaccharides, 2-5 parts of synergist, and 1-2 parts of trace elements.

[0009] The reaction mechanism and effects of the present invention are as follows:

[0010] The present invention utilizes enzymatic hydrolysis combined with fermentation to convert cockroach body waste into cockroach-based fertilizer. The applicant has found that strictly controlling the process parameters during enzymatic hydrolysis and fermentation is beneficial to increasing the content of amino acids in the cockroach body waste.

[0011] First, the present invention selects a specific amount of papain to enzymolyze the powder (cockroach powder, peel powder and oatmeal powder) after crushing, and regulates the enzymolysis process by controlling enzyme activity, enzymolysis time, enzymolysis temperature, environmental pH and substrate concentration, and degrades the macromolecules that are difficult to degrade in cockroach body waste and apple peel into small molecules. Secondly, the present invention selects a specific amount of alpha-amylase for enzymolysis, mainly to further enzymolyze the crushed oatmeal powder to produce beta-glucan, which helps to produce adhesion, so that the stability of the fertilizer produced subsequently is good. Secondly, the applicant controls the addition amount of mixed bacteria seed liquid and the type and ratio of mixed bacteria, which is conducive to the absorption of small molecules by mixed bacteria, and converts them into amino acids and polypeptides, thereby improving the utilization rate of raw materials. Then, the present invention achieves the optimization of the growth environment of mixed microorganisms by accurately regulating the mass ratio between cockroach powder, peel powder and oatmeal powder. In this way, the peel powder can provide the necessary carbon source and nutrients for the mixed microorganisms, promoting them to decompose the cockroach powder more effectively; at the same time, this also avoids excessive nutrient supply and prevents competitive inhibition between microorganisms, which may cause the conversion efficiency of cockroach powder to decrease. Therefore, the present invention can effectively maintain the stability of the amino acid content in the cockroach-based fertilizer, thereby improving the overall conversion efficiency and product quality.

[0012] In addition, the present invention adds a fertilizer synergist to improve the utilization rate of fertilizer. Rhamnose gum helps to improve the physical stability and biological activity of fertilizers, and promotes the growth and development of plant roots. Polyglutamic acid can improve the utilization rate of nitrogen fertilizers by slowing down the loss of nitrogen. N-methyl-3-aminopyrazole helps to reduce the volatilization and leaching losses of nitrogen fertilizers by inhibiting nitrification, improves the absorption and utilization of nitrogen and other medium and trace elements in the soil by crops, and thus improves the growth and yield of crops.

[0013] In some embodiments, the method for preparing the cockroach-based fertilizer comprises the following steps:

[0014] T1. Wash the waste cockroach body, dry it in the sun, and crush it to obtain cockroach powder; wash the apple peel, dry it in the sun, and crush it to obtain peel powder; wash the oat bran, dry it in the sun, crush it, add distilled water, heat it to 40-50℃, ultrasonically treat it, centrifuge it, take the supernatant and dry it to obtain oat powder;

[0015] T2. Mix the cockroach powder, peel powder and oatmeal powder obtained in step T1, add distilled water, heat to 45-50°C, stir, cool, sterilize, add papain, stir, adjust the pH to 6-6.5, heat to 45-60°C and hydrolyze for 2-5h, add α-amylase, heat to 90-95°C and hydrolyze for 30-45min, kill the enzyme, and obtain a cockroach-based enzymatic solution;

[0016] T3. The pH of the cockroach-based enzymatic hydrolysate obtained in step T2 is adjusted to 6.5-7.0, the mixed bacterial seed solution is inoculated therein, fermented at 30-37° C. for 5-10 days, filtered, and sterilized to obtain a cockroach-based fertilizer.

[0017] In some embodiments, the material-liquid ratio in step T2 is 1 g: (4-6) mL, wherein the material is cockroach powder, peel powder and oatmeal powder, and the liquid is distilled water.

[0018] In some embodiments, the papain and α-amylase in step T2 account for 0.8-1.4 wt % and 1.5-1.8 wt % of the cockroach powder, respectively.

[0019] In some embodiments, the mass ratio of the cockroach powder, the peel powder and the oatmeal powder in step T2 is (45-60):1:(12-20).

[0020] Preferably, the mass ratio of the cockroach powder, the peel powder and the oatmeal powder in step T2 is 55:1:16.

[0021] In some embodiments, the mixed bacterial seed solution in step T3 has a concentration of (1-9)×10 8-9The composition comprises a bifidobacterium seed solution, a bacillus seed solution and an Aspergillus oryzae seed solution with a CFU / mL, wherein the volume ratio of the three is (1-2):1:(1.5-2).

[0022] In some embodiments, the bifidobacterium seed solution is a Bifidobacterium lactis seed solution, and the Bacillus seed solution is a Bacillus subtilis seed solution.

[0023] In some embodiments, the amount of the mixed bacterial seed solution in step T3 is 2-5 wt % of the cockroach-based enzymatic hydrolysate.

[0024] In some embodiments, each portion of the trace elements comprises the following raw materials by weight: 3-6 portions of magnesium sulfate, 1-2 portions of ferrous sulfate, 1-2 portions of copper sulfate, 1-2 portions of zinc sulfate, 1-1.5 portions of manganese sulfate and 1-1.5 portions of boric acid.

[0025] In some embodiments, each portion of the synergist comprises the following raw materials by weight: 0.5-1 portion of rhamnose gum, 4-8 portions of polyglutamic acid, and 1-2 portions of N-methyl-3-aminopyrazole.

[0026] Another aspect of the present invention provides a method for preparing organic fertilizer using cockroach waste, comprising the following steps:

[0027] Phosphorus fertilizer, potassium fertilizer, urea and brown algae oligosaccharide are mixed, and then cockroach-based fertilizer, synergist and trace elements are added to obtain organic fertilizer.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] 1. The organic fertilizer of the present invention has a high degradation rate of cockroach body waste, contains rich amino acids after enzymatic hydrolysis and fermentation, has a simple production process, has good stability, can significantly improve resource utilization and the quality of crops, and has both social and economic benefits.

[0030] 2. The present invention selects bifidobacterium, bacillus and Aspergillus oryzae in a specific ratio to improve the selectivity of the mixed bacteria to cockroach powder, has a better bioavailability of cockroach powder, and thus increases the amino acid content.

[0031] 3. The present invention uses a synergist to improve the utilization rate of fertilizers, which has a good activation effect on nitrogen and other medium and trace elements in the soil, can promote plant absorption and the growth and development of plant roots, and has the advantages of high efficiency and safety. DETAILED DESCRIPTION

[0032] The present invention will be described below in conjunction with specific embodiments. It should be noted that the following embodiments are examples of the present invention and are only used to illustrate the present invention, rather than to limit the present invention. Other combinations and various modifications within the concept of the present invention may be performed without departing from the spirit or scope of the present invention.

[0033] According to the ratios and preparation methods of the raw materials specified in the following examples and comparative examples, various organic fertilizers were prepared.

[0034] In order to facilitate those skilled in the art to implement the present invention, some raw material manufacturers of the embodiments and comparative examples are described as follows:

[0035] The bifidobacterium was Bifidobacterium lactis BIOMA 66, which was deposited in the Sichuan Microbial Resources Platform Strain Collection Center;

[0036] The Bacillus was Bacillus subtilis CICC 10275, which was deposited in the China Medical Microbiology Culture Collection Center;

[0037] Aspergillus oryzae was Aspergillus oryzae CICC 10275, deposited in Sichuan Food Fermentation Industry Research and Design Institute;

[0038] Papain: purchased from Shanghai Quanyan Biotechnology Co., Ltd., with an activity of 400,000 U / g;

[0039] α-Amylase: purchased from Hubei Meite Chemical Co., Ltd., thermostable α-amylase, activity of 20000U / g;

[0040] MRS liquid culture medium: purchased from Shanghai Yuchun Biotechnology Co., Ltd.;

[0041] LB liquid medium: purchased from Guangdong Huankai Microbiological Technology Co., Ltd.;

[0042] Potato Dextrose Liquid Medium:

[0043] Peel the potatoes and cut them into 1cm pieces 3 100 g of potato pieces were put into 500 mL of deionized water and boiled for 30 min, filtered with double-layer gauze, 10 g of glucose was added to the filtrate, and deionized water was added to 500 mL, the pH was adjusted to 7 with 1 mol / L sodium hydroxide solution, sterilized at 120 ° C for 20 min, and naturally cooled to room temperature to obtain a potato glucose liquid culture medium;

[0044] Phosphate fertilizer, potash fertilizer, urea: all are liquid fertilizers;

[0045] Other raw materials are not specially specified and can be purchased from the market.

[0046] Preparation Example 1

[0047] The preparation steps of Bacillus subtilis seed solution are as follows:

[0048] (1) Under a sterile environment, 100 μL of bacterial solution was taken from the glycerol tube containing the Bacillus subtilis strain, and evenly spread on the LB plate medium by the plate streak method, and inverted and cultured at 37° C. until colonies grew, thereby obtaining activated Bacillus subtilis;

[0049] (2) In a sterile environment, the activated Bacillus subtilis was inoculated into 500 mL of LB liquid medium, and cultured at 37°C and 100 rpm for 2 days. The culture was centrifuged at 5000 r / min to obtain the cells, which were diluted with sterile water to 6 × 10 9 CFU / mL, and obtain the Bacillus subtilis seed solution.

[0050] Preparation Example 2

[0051] The preparation steps of Bifidobacterium lactis seed solution are as follows:

[0052] (1) Under a sterile environment, 100 μL of bacterial solution was drawn from a glycerol tube storing the Bifidobacterium lactis strain, and evenly spread on an LB plate medium by a plate streak method, and inverted and cultured at 37° C. until colonies grew, thereby obtaining activated Bifidobacterium lactis;

[0053] (2) Under a sterile environment, the activated Bifidobacterium lactis was inoculated into 500 mL of MRS liquid culture medium, and cultured at 37°C and 100 rpm for 1 day, followed by centrifugation at 5000 r / min to obtain the bacterial cells, which were diluted with sterile water to 6 × 10 9 CFU / mL, and obtain the seed liquid of Bifidobacterium lactis.

[0054] Preparation Example 3

[0055] The preparation steps of Aspergillus oryzae seed solution are as follows:

[0056] (1) In a sterile environment, a 0.5 cm×0.5 cm bacterial block was inoculated on a PDA slant medium with the stored Aspergillus oryzae mother culture, and cultured at 37° C. until the mycelium was full, thereby obtaining activated Aspergillus oryzae;

[0057] (2) Under a sterile environment, the activated Aspergillus oryzae was inoculated into 500 mL of potato dextrose liquid medium, and cultured at 37°C and 100 rpm for 3 days, followed by centrifugation at 5000 r / min to obtain the bacterial cells, which were diluted with sterile water to 6 × 10 9 CFU / mL, and Aspergillus oryzae seed liquid was obtained.

[0058] Preparation Example 4

[0059] The preparation method of cockroach-based fertilizer A comprises the following steps:

[0060] T1. Wash the waste cockroach body, dry it in the sun, and crush it to obtain cockroach powder; wash the apple peel, dry it in the sun, and crush it to obtain peel powder; wash the oat bran, dry it in the sun, crush it, add distilled water 10 times the mass of the oat bran, heat it to 45°C, ultrasonically treat it, centrifuge it, take the supernatant, and dry it at 85°C for 6 hours to obtain oat powder;

[0061] T2. Mix 550g cockroach powder, 10g peel powder and 160g oatmeal powder obtained in step T1, add 3600mL distilled water, heat to 45°C, stir for 30min, cool to room temperature, sterilize at 130°C for 20min, add 6g papain, stir for 30min, adjust pH to 6, heat to 50°C and hydrolyze for 4h, add 9g α-amylase, heat to 95°C and hydrolyze for 35min, and inactivate the enzyme at 130°C for 10min to obtain a cockroach-based enzymatic solution;

[0062] T3. The pH of the cockroach-based hydrolyzate obtained in step T2 was adjusted to 7.0, 3.5 wt% of the mixed bacterial seed solution was inoculated therein, fermented at 37 ° C for 7 days, filtered, and sterilized at 130 ° C for 10 min to obtain cockroach-based fertilizer A;

[0063] The mixed bacteria seed solution includes the Bifidobacterium lactis seed solution of Preparation Example 1, the Bacillus subtilis seed solution of Preparation Example 2 and the Aspergillus oryzae seed solution of Preparation Example 3 in a volume ratio of 1.5:1:2.

[0064] Preparation Example 5

[0065] The preparation method of cockroach-based fertilizer B comprises the following steps:

[0066] T1. Wash and dry the cockroach waste, crush it to obtain cockroach powder; wash and dry the apple peel, crush it to obtain peel powder;

[0067] T2. 550 g of cockroach powder, 10 g of peel powder and 160 g of oatmeal powder obtained in step T1 were mixed, 3600 mL of distilled water was added, heated to 45 ° C, stirred for 30 min, cooled to room temperature, and sterilized at 130 ° C for 20 min to obtain a cockroach-based enzymatic solution;

[0068] T3. The pH of the cockroach-based enzymatic hydrolyzate obtained in step T2 was adjusted to 7.0, 3.5 wt% of the mixed bacterial seed solution was inoculated therein, fermented at 37 ° C for 7 days, filtered, and sterilized at 130 ° C for 10 min to obtain cockroach-based fertilizer B;

[0069] The mixed bacteria seed solution includes the Bifidobacterium lactis seed solution of Preparation Example 1, the Bacillus subtilis seed solution of Preparation Example 2 and the Aspergillus oryzae seed solution of Preparation Example 3 in a volume ratio of 1.5:1:2.

[0070] Preparation Example 6

[0071] The preparation method of cockroach-based fertilizer C is the same as that of Preparation Example 4, except that the amount of papain added in step T2 is 3.5 g.

[0072] Preparation Example 7

[0073] The preparation method of cockroach-based fertilizer D is the same as that of Preparation Example 4, except that the amount of α-amylase added in step T2 is 7.2 g.

[0074] Preparation Example 8

[0075] The preparation method of cockroach-based fertilizer E is the same as that of Preparation Example 4, except that in step T2, 430 g of cockroach powder, 10 g of fruit peel powder and 280 g of oatmeal powder are mixed instead of 550 g of cockroach powder, 10 g of fruit peel powder and 160 g of oatmeal powder.

[0076] Preparation Example 9

[0077] The preparation method of cockroach-based fertilizer F is the same as that of Preparation Example 4, except that the mixed bacterial seed solution in step T3 includes the Bifidobacterium lactis seed solution of Preparation Example 1, the Bacillus subtilis seed solution of Preparation Example 2, and the Aspergillus oryzae seed solution of Preparation Example 3 in a volume ratio of 1:1:1.

[0078] Preparation Example 10

[0079] The preparation method of cockroach-based fertilizer G is the same as that of Preparation Example 4, except that the mixed bacterial seed solution in step T3 includes the Bifidobacterium lactis seed solution of Preparation Example 1 and the Bacillus subtilis seed solution of Preparation Example 2 in a volume ratio of 1.5:1.

[0080] Preparation Example 11

[0081] The preparation method of cockroach-based fertilizer H is the same as that of Preparation Example 4, except that the amount of the mixed bacterial seed solution added in step T3 is 1.8 wt %.

[0082] Example 1

[0083] An organic fertilizer made from cockroach waste, comprising the following raw materials by weight: 70 parts of cockroach-based fertilizer A, 22.5 parts of phosphate fertilizer, 17.5 parts of potash fertilizer, 15 parts of urea, 4 parts of brown algae oligosaccharide, 3.5 parts of synergist, and 1.5 parts of trace elements; wherein:

[0084] Each portion of trace elements contains the following raw materials: 4.5 parts of magnesium sulfate, 1.5 parts of ferrous sulfate, 1.5 parts of copper sulfate, 1.5 parts of zinc sulfate, 1.2 parts of manganese sulfate and 1.2 parts of boric acid;

[0085] Each part of the synergist contains the following ingredients: 0.75 parts of rhamnose gum, 6 parts of polyglutamic acid, and 1.5 parts of N-methyl-3-aminopyrazole;

[0086] The method for preparing the organic fertilizer of this embodiment comprises the following steps:

[0087] The phosphate fertilizer, potash fertilizer, urea and brown algae oligosaccharide were mixed and stirred for 30 minutes, and then the cockroach-based fertilizer A, synergist and trace elements were added and mixed, and stirred for 1 hour to obtain organic fertilizer.

[0088] Example 2

[0089] An organic fertilizer made from cockroach waste, comprising the following raw materials by weight: 60 parts of cockroach-based fertilizer A, 15 parts of phosphate fertilizer, 10 parts of potash fertilizer, 10 parts of urea, 3 parts of brown algae oligosaccharide, 2 parts of synergist, and 1 part of trace elements; wherein:

[0090] Each trace element contains the following ingredients: 3 parts magnesium sulfate, 1 part ferrous sulfate, 1 part copper sulfate, 1 part zinc sulfate, 1 part manganese sulfate and 1 part boric acid;

[0091] Each part of the enhancer contains the following ingredients: 0.5 parts of rhamnose gum, 4 parts of polyglutamic acid, 1 part of N-methyl-3-aminopyrazole;

[0092] The preparation method of the organic fertilizer of this embodiment is the same as that of Example 1.

[0093] Example 3

[0094] An organic fertilizer made from cockroach waste, comprising the following raw materials by weight: 80 parts of cockroach-based fertilizer A, 30 parts of phosphate fertilizer, 25 parts of potash fertilizer, 20 parts of urea, 5 parts of brown algae oligosaccharide, 5 parts of synergist, and 2 parts of trace elements; wherein:

[0095] Each portion of trace elements contains the following ingredients: 6 parts of magnesium sulfate, 2 parts of ferrous sulfate, 1 part of copper sulfate, 1 part of zinc sulfate, 1.5 parts of manganese sulfate and 1.5 parts of boric acid;

[0096] Each synergist contains the following ingredients: 1 part of rhamnose gum, 8 parts of polyglutamic acid, 2 parts of N-methyl-3-aminopyrazole;

[0097] The preparation method of the organic fertilizer of this embodiment is the same as that of Example 1.

[0098] Example 4

[0099] An organic fertilizer using cockroach body waste and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of cockroach-based fertilizer B is used to replace cockroach-based fertilizer A.

[0100] Example 5

[0101] An organic fertilizer using cockroach body waste and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of cockroach-based fertilizer C is used to replace cockroach-based fertilizer A.

[0102] Example 6

[0103] An organic fertilizer using cockroach body waste and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of cockroach-based fertilizer D is used to replace cockroach-based fertilizer A.

[0104] Example 7

[0105] An organic fertilizer using cockroach body waste and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of cockroach-based fertilizer E is used to replace cockroach-based fertilizer A.

[0106] Example 8

[0107] An organic fertilizer using cockroach body waste and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of cockroach-based fertilizer F is used to replace cockroach-based fertilizer A.

[0108] Example 9

[0109] An organic fertilizer using cockroach body waste and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of cockroach-based fertilizer G is used to replace cockroach-based fertilizer A.

[0110] Example 10

[0111] An organic fertilizer using cockroach body waste and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of cockroach-based fertilizer H is used to replace cockroach-based fertilizer A.

[0112] Embodiment 11

[0113] An organic fertilizer using cockroach body waste and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that each portion of synergist contains 0.75 portions of rhamnose gum and 6 portions of polyglutamic acid.

[0114] Comparative Example 1

[0115] An organic fertilizer using cockroach body waste and a preparation method thereof, wherein the specific implementation manner is the same as that of Example 1, except that an equal amount of cockroach powder obtained in step T1 of Preparation Example 4 is used to replace cockroach-based fertilizer A.

[0116] Effect evaluation:

[0117] The organic fertilizers prepared in the above Examples 1-11 and Comparative Example 1 were tested, and the specific results are shown in Tables 1 and 2.

[0118] Performance Test:

[0119] (1) The cockroach-based fertilizers obtained in Preparation Examples 4, 5, and 9 were tested, and the amino acid content was determined by GC-MS. The results are shown in Table 1.

[0120] Table 1

[0121] Serial number Amino Acids (mg / mL) Preparation Example 4 4.65 Preparation Example 5 2.80 Preparation Example 9 4.02

[0122] As shown in Table 1, the amino acid content of cockroach-based fertilizer A obtained in Preparation Example 4 is the highest, which is beneficial to improving the quality of crops. Preparation Example 5 did not perform enzymatic hydrolysis, and Preparation Example 9 changed the volume ratio of the mixed bacteria seed liquid, both of which reduced the amino acid content.

[0123] (2) Crop experiments

[0124] The obtained organic fertilizer was used for Hami melon planting test, and the results are shown in Table 2:

[0125] Using soil cultivation, the plot with uniform fertility was divided into 12 experimental plots, each with an area of ​​30m 2 , spray 5kg of 300-fold diluted organic fertilizer every 14 days. In addition to spraying organic fertilizer, other conventional management measures remain the same throughout the planting process;

[0126] Experimental variety: Fort Red Hami melon; 3 vines were retained on each plant, 25 plants were selected from each treatment, and the average value was calculated as the single melon weight. Five Hami melons that matured at the same time were randomly selected from each experimental plot, and the sugar content of the Hami melons was tested using a digital saccharimeter and the average value was taken.

[0127] Table 2

[0128]

[0129]

[0130] The results in Table 1 show that the organic fertilizers of Examples 1-3 can improve the fruit quality of Paotai Red Hami melon, and both the single melon weight and the sugar content are significantly improved.

[0131] Compared with Example 1, Examples 4-10 all changed the parameters in the preparation process of cockroach-based fertilizers. Example 4 did not perform enzymatic hydrolysis, which was not conducive to subsequent fermentation. Examples 5-6 respectively changed the amount of papain and α-amylase added, resulting in a low enzymatic hydrolysis rate. Example 7 changed the mass ratio of cockroach powder, peel powder and oatmeal powder, which may cause competitive inhibition between microorganisms. Example 8 changed the volume ratio of the mixed bacteria seed liquid, Example 9 changed the type of the mixed bacteria seed liquid, and Example 10 changed the amount of the mixed bacteria seed liquid added. Examples 8-10 all affected the fermentation process to varying degrees, and further, Examples 4-10 all caused the weight of a single melon and the sugar content to decrease to varying degrees.

[0132] Compared with Example 1, Example 11 changed the type of synergist, and the fertilizer utilization rate decreased. Comparative Example 1 used the cockroach powder obtained in step T1 of Preparation Example 4 to replace the cockroach-based fertilizer A without enzymatic hydrolysis and fermentation, which would reduce the single melon weight and sugar content.

[0133] The above is only a preferred embodiment of the present invention and does not constitute any form of limitation to the present application. Although the present application is disclosed as above in the preferred embodiment, it is not intended to limit the present application. Any technician familiar with the profession, without departing from the scope of the technical solution of the present application, using the technical content disclosed above to make slight changes or modifications are equivalent to equivalent implementation cases. However, any simple modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution.

Claims

1. An organic fertilizer made from cockroach waste, characterized in that: The raw materials are as follows, measured by weight: 60-80 parts of cockroach-based fertilizer, 15-30 parts of phosphate fertilizer, 10-25 parts of potash fertilizer, 10-20 parts of urea, 3-5 parts of brown algae oligosaccharides, 2-5 parts of synergist, and 1-2 parts of trace elements.

2. The organic fertilizer made from cockroach waste according to claim 1, characterized in that: The method for preparing the cockroach-based fertilizer comprises the following steps: T1. Wash the waste cockroach body, dry it in the sun, and crush it to obtain cockroach powder; wash the apple peel, dry it in the sun, and crush it to obtain peel powder; wash the oat bran, dry it in the sun, crush it, add distilled water, heat it to 40-50℃, ultrasonically treat it, centrifuge it, take the supernatant and dry it to obtain oat powder; T2. Mix the cockroach powder, peel powder and oatmeal powder obtained in step T1, add distilled water, heat to 45-50°C, stir, cool, sterilize, add papain, stir, adjust the pH to 6-6.5, heat to 45-60°C and hydrolyze for 2-5h, add α-amylase, heat to 90-95°C and hydrolyze for 30-45min, kill the enzyme, and obtain a cockroach-based enzymatic solution; T3. The pH of the cockroach-based enzymatic hydrolysate obtained in step T2 is adjusted to 6.5-7.0, the mixed bacterial seed solution is inoculated therein, fermented at 30-37° C. for 5-10 days, filtered, and sterilized to obtain a cockroach-based fertilizer.

3. The organic fertilizer using cockroach waste according to claim 2, characterized in that: In step T2, the papain and α-amylase account for 0.8-1.4wt% and 1.5-1.8wt% of the cockroach powder respectively.

4. The organic fertilizer using cockroach waste according to claim 2, characterized in that: The mass ratio of the cockroach powder, the peel powder and the oatmeal powder in step T2 is (45-60):1:(12-20).

5. The organic fertilizer using cockroach waste according to claim 2, characterized in that: The mixed bacterial seed solution in step T3 has a concentration of (1-9)×10 8-9 The composition comprises a bifidobacterium seed solution, a bacillus seed solution and an Aspergillus oryzae seed solution with a CFU / mL, wherein the volume ratio of the three is (1-2):1:(1.5-2).

6. The organic fertilizer using cockroach waste according to claim 5, characterized in that: The bifidobacterium seed liquid is a Bifidobacterium lactis seed liquid, and the bacillus seed liquid is a Bacillus subtilis seed liquid.

7. The organic fertilizer using cockroach waste according to claim 5, characterized in that: The amount of the mixed bacterial seed solution in step T3 is 2-5wt% of the cockroach-based enzymatic hydrolysate.

8. The organic fertilizer using cockroach waste according to claim 1, characterized in that: Each portion of the trace elements comprises the following raw materials by weight: 3-6 portions of magnesium sulfate, 1-2 portions of ferrous sulfate, 1-2 portions of copper sulfate, 1-2 portions of zinc sulfate, 1-1.5 portions of manganese sulfate and 1-1.5 portions of boric acid.

9. The organic fertilizer using cockroach waste according to claim 1, characterized in that: Each portion of the synergist comprises the following raw materials by weight: 0.5-1 portion of rhamnose gum, 4-8 portions of polyglutamic acid, and 1-2 portions of N-methyl-3-aminopyrazole.

10. A method for preparing organic fertilizer using cockroach waste as claimed in any one of claims 1 to 9, characterized in that: The following steps are included: Phosphorus fertilizer, potassium fertilizer, urea and brown algae oligosaccharide are mixed, and then cockroach-based fertilizer, synergist and trace elements are added to obtain organic fertilizer.

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