Preparation method and application of saline-alkali-resistant soil conditioner taking mixed algae as raw material

Abstract

The invention discloses a preparation method and application of a saline-alkali-resistant soil conditioner taking mixed algae as a raw material. The saline-alkali-resistant soil conditioner is specifically prepared from the following raw materials in parts by weight: 50-80 parts of mixed algae powder; 10 to 30 parts of humic acid; and 5-10 parts of a microbial agent. The invention relates to the technical field of soil conditioning, in particular to a preparation method and application of a saline-alkali-resistant soil conditioner taking mixed algae as a raw material, the soil salinity and alkalinity of saline-alkali soil can be obviously reduced, for example, in the later growth period of wheat, the soil salinity and alkalinity can be reduced to 0.2%-0.3% from the initial 0.5%-1.0%, the reduction amplitude reaches 50%-60%, and the soil salinity and alkalinity can be obviously improved. The soil fertility is greatly improved, the conditioner can remarkably increase the content of nutrients such as organic matters, nitrogen, phosphorus and potassium in soil, meanwhile, the pH value of the soil is reduced, the pH value of the soil is improved, the content of the organic matters can be increased by 50%, the content of the nitrogen can be increased by 40%, the content of the phosphorus can be increased by 40%, and the content of the potassium can be increased by 30%. The potassium content is increased by 33.3%, the PH value is reduced by 4.88%, and compared with a commercially available conditioner, the soil fertility index is increased by the conditioner.

Description

Technical Field

[0001] The invention relates to the technical field of soil conditioning, and in particular to a preparation method and application of a salt-alkali resistant soil conditioner using mixed algae as a raw material. Background Art

[0002] Soil salinization is a global ecological problem that has a serious impact on agricultural production and the ecological environment. With the growth of population and economic development, the demand for land resources continues to increase, and the existence of saline-alkali land has seriously restricted the effective use of land. At present, there are about 950 million hectares of saline-alkali land in the world, and there are also a large number of saline-alkali lands in my country, such as Northwest, North China and Northeast China.

[0003] At present, soil salinization is a global environmental problem. The high salinity of the soil has seriously affected the growth and yield of crops. In traditional agricultural production, chemical and organic conditioners are often used to improve saline-alkali land, but these conditioners often have some limitations.

[0004] Algae have diverse living environments. Most of them grow in water, and a few live in the shade and wet places on land. They can live in fresh water, sea water, moist soil or rock surface. Algae have a simple structure, and some are single-celled, such as Chlamydomonas. Wide distribution and easy cultivation are the basis for algae to be used as raw materials.

[0005] Reasons why algae can be used as a saline-alkali land improver:

[0006] Some algae with salt-resistant properties can effectively remove salt from saline-alkali soil by utilizing their ability to absorb salt. Algae can maintain growth and life activities by absorbing salt, transferring salt and inorganic salts from the soil into the algae, thereby reducing the salt content in the soil. In this way, the salt in saline-alkali soil can be removed and the soil's production capacity can be restored.

[0007] Improve soil structure: Living algae has a special symbiotic relationship, which can cooperate with other organisms in the soil to promote the improvement of soil structure. Living algae can improve the pH of the soil through secretions, increase soil permeability, thereby improving the water utilization efficiency of the land and promoting plant growth.

[0008] Although commercially available chemical conditioners can reduce soil salinity to a certain extent, the reduction is small, usually 20%-30%. At the same time, chemical conditioners may have adverse effects on the soil ecological environment, such as destroying the balance of soil microbial communities and polluting groundwater.

[0009] Commercially available organic conditioners also have a certain effect in improving soil, but the extent to which they reduce soil salinity is generally 30%-40%, and the effect is still unsatisfactory. In addition, the cost of organic conditioners is relatively high, and the sources are not widespread, which limits their application in large-scale agricultural production.

[0010] To this end, the present invention provides a preparation method and application of a salt-alkali resistant soil conditioner using mixed algae as raw materials. Summary of the invention

[0011] In view of the deficiencies in the prior art, the present invention provides a method for preparing a salt-alkali resistant soil conditioner using mixed algae as raw materials and its application, which solves the problems of the existing salt-alkali resistant soil conditioners such as unsatisfactory effects, high costs and easy pollution.

[0012] To achieve the above objectives, the present invention is implemented through the following technical scheme: a salt-alkali resistant soil conditioner using mixed algae as raw materials: specifically composed of the following raw materials in parts by weight:

[0013] Mixed algae powder 50-80 parts;

[0014] Humic acid 10-30 parts;

[0015] 5-10 parts of microbial agent;

[0016] 1-5 portions of trace elements.

[0017] Preferably, it is characterized in that the mixed algae is a mixture of Spirulina, Chlorella and Nostoc, and the weight ratio thereof is 12:11:10.

[0018] Preferably, it is characterized in that the microbial agent is a mixture of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium in a weight ratio of 10:10:10.

[0019] Preferably, the trace elements are a mixture of iron, zinc, boron and molybdenum in a weight ratio of 10:11:10:12.

[0020] Preferably, the preparation method of the salt-alkali resistant soil conditioner using mixed algae as raw materials is:

[0021] 1) Cultivation of mixed algae:

[0022] A. Spirulina culture:

[0023] a1. Preparation of culture medium: Sodium bicarbonate (16.8 g / L), dipotassium hydrogen phosphate (0.5 g / L), sodium nitrate (2.5 g / L), potassium sulfate (1.0 g / L), sodium chloride (1.0 g / L), magnesium sulfate heptahydrate (0.2 g / L), calcium chloride dihydrate (0.04 g / L), ferrous sulfate heptahydrate (0.01 g / L), and disodium ethylenediaminetetraacetic acid (0.08 g / L) were dissolved in distilled water in accordance with the formula ratio, the pH value was adjusted to 9.0, and the mixture was sterilized by high pressure sterilization in an autoclave, and cooled for use;

[0024] a2. Inoculation and culture: Take the Spirulina algae seeds and inoculate them into a glass culture bottle or plastic culture bucket filled with culture medium, with an inoculation amount of 10%-15%. Cultivate at a temperature of 25-30℃, a light intensity of 2000-3000lx, and a light duration of 12-16h / d. During the cultivation process, use a stirrer to regularly stir the culture solution;

[0025] a3. Collection and drying: When the concentration of Spirulina cells reaches 0.6-0.8 at a specific wavelength measured by a spectrophotometer, the cells are collected by centrifugation at 3000-4000 r / min for 10-15 min. The collected Spirulina cells are dried for 24-48 h using a freeze dryer at a drying temperature of -40°C to -50°C and a vacuum degree of 10-20 Pa. The dried Spirulina is crushed using a pulverizer and passed through a 100-mesh sieve to obtain Spirulina powder for later use.

[0026] B. Cultivation of Chlorella:

[0027] b1. Preparation of culture medium: Sodium nitrate (1.5 g / L), dipotassium hydrogen phosphate trihydrate (0.04 g / L), magnesium sulfate heptahydrate (0.075 g / L), calcium chloride dihydrate (0.036 g / L), citric acid (0.006 g / L), ammonium ferric citrate (0.006 g / L), disodium ethylenediaminetetraacetate (0.001 g / L), and sodium carbonate (0.02 g / L) were dissolved in distilled water in accordance with the formula ratio, the pH value was adjusted to 7.0, and the culture medium was sterilized by high pressure sterilization pot, and cooled for use;

[0028] b2. Inoculation and culture: Take the Chlorella algae and inoculate it into a glass culture bottle or plastic culture barrel filled with culture medium, with an inoculation amount of 5%-10%. Cultivate at a temperature of 25-30℃, a light intensity of 2000-3000lx, and a light duration of 12-16h / d. During the cultivation process, use a stirrer to regularly stir the culture solution;

[0029] b3. Collection and drying: When the concentration of Chlorella cells reaches 0.4-0.65 when the absorbance of the culture solution at a specific wavelength is measured by a spectrophotometer, the cells are collected by centrifugation at a speed of 3000-4000 r / min for 10-15 min. The collected Chlorella cells are dried for 24-48 h using a freeze dryer at a drying temperature of -40°C to -50°C and a vacuum degree of 10-20 Pa. The dried Chlorella is crushed using a pulverizer and passed through a 100-mesh sieve to obtain Chlorella powder for later use.

[0030] C. Nostoc culture:

[0031] c1. Culture medium preparation: same as that of Chlorella vulgaris;

[0032] c2. Inoculation and culture: Take the Nostoc algae seeds and inoculate them into a glass culture bottle or plastic culture bucket filled with culture medium, with an inoculation amount of 5%-10%. Cultivate at a temperature of 25-30℃, a light intensity of 2000-3000lx, and a light duration of 12-16h / d. During the cultivation process, use a stirrer to regularly stir the culture solution;

[0033] c3. Collection and drying: When the concentration of Nostoc cells reaches 0.4-0.65 when the absorbance of the culture solution at a specific wavelength is measured by a spectrophotometer, the cells are collected by centrifugation at a speed of 3000-4000r / min for 10-15min. The collected Nostoc cells are dried for 24-48h using a freeze dryer at a drying temperature of -40°C to -50°C and a vacuum degree of 10-20Pa. The dried Nostoc is crushed using a pulverizer and passed through a 100-mesh sieve to obtain Nostoc powder.

[0034] 2) Preparation of mixed algae powder: Spirulina powder, Chlorella powder and Nostoc powder are mixed in a weight ratio of 1:1:1 to obtain mixed algae powder.

[0035] 3) Humic acid crushing: crush the humic acid using a ball mill, crusher or other equipment, and pass it through an 80-mesh sieve. Alternatively, use an airflow crushing method to crush the humic acid into the required particle size using an airflow crusher.

[0036] 4) Crushing of microbial agents and trace elements:

[0037] D. Crushing of microbial agents: Crushing the microbial agents using a ball mill, a pulverizer, or other equipment, and passing through a 100-mesh sieve. Alternatively, a freezing crushing method is used, where the microbial agents are frozen at low temperatures and then crushed using a freezing crusher. The microbial agents are a mixture of Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium, with a weight ratio of 10:13:15.

[0038] F. Pulverizing trace elements: Pulverize the trace elements using a ball mill, a pulverizer, or the like, and pass through a 100-mesh sieve, or use an ultrasonic pulverization method to pulverize the trace elements to a desired particle size using an ultrasonic pulverizer. The trace elements are a mixture of iron, zinc, boron, and molybdenum, and the weight ratio thereof is 10:10:10:10.

[0039] 5) Mixing preparation: the mixed algae powder obtained in step 1, the crushed humic acid powder, the crushed microbial agent powder and the trace element powder are mixed evenly by weight, and 5-10 parts by weight of urea are added to supplement the nitrogen element to obtain the salt-alkali resistant soil conditioner. The mixing method adopts a mechanical stirring method, using a mixer to stir the various raw materials evenly; or an air flow mixing method is adopted, using an air flow mixer to mix the various raw materials evenly.

[0040] Preferably, the culture conditions of Spirulina, Chlorella and Nostoc are: temperature 25-30°C, light intensity 2000-3000lx, and illumination time 12-16h / d.

[0041] Preferably, in step 1), the drying is freeze-drying.

[0042] Preferably, the application of the salt-alkali resistant soil conditioner using mixed algae as raw materials includes the following:

[0043] S1. Application of base fertilizer: Before planting, apply the conditioner as base fertilizer to the soil and mix it thoroughly with the soil;

[0044] S2. Topdressing: During the growth of plants, topdressing is applied;

[0045] S3. Foliar spraying: dilute the conditioner and spray it on the leaves.

[0046] Beneficial Effects

[0047] The present invention provides a preparation method and application of a salt-alkali resistant soil conditioner using mixed algae as raw materials. Compared with the prior art, it has the following beneficial effects:

[0048] (1) The preparation method and application of the salt-alkali resistant soil conditioner using mixed algae as raw materials can significantly reduce the soil salinity of saline-alkali land. For example, in the late stage of wheat growth, the soil salinity can be reduced from the initial 0.5%-1.0% to 0.2%-0.3%, a reduction of 50%-60%, which is much higher than the commercially available chemical conditioners (reduction of 20%-30%) and organic conditioners (reduction of 30%-40%), greatly improving soil fertility: the conditioner can significantly increase the content of nutrients such as organic matter, nitrogen, phosphorus, and potassium in the soil, while reducing the soil pH value and improving the soil acidity and alkalinity. Among them, the organic matter content can be increased by 50%, the nitrogen content by 40%, the phosphorus content by 40%, the potassium content by 33.3%, and the pH value by 4.88%, while the commercially available conditioners have a smaller improvement on soil fertility indicators.

[0049] (2) The preparation method and application of the salt-alkali resistant soil conditioner with mixed algae as raw materials. The conditioner with mixed algae as the main raw material has a wide source and has no adverse effects on the soil microbial community and the surrounding ecological environment during use. For example, it can promote the growth and reproduction of beneficial microorganisms in the soil, improve the diversity of soil microorganisms, and meet the requirements of sustainable development. In addition, mixed algae and other ingredients are relatively easy to obtain, and the cultivation, crushing and mixing methods used in the preparation process are relatively conventional and the cost is relatively low. Compared with commercially available conditioners, while achieving good salt-alkali resistance and promoting crop growth, it has obvious cost advantages, which provides the possibility for large-scale promotion and application. BRIEF DESCRIPTION OF THE DRAWINGS

[0050] Figure 1 The following is a formula table of the mixed algae of the present invention.

[0051] Figure 2 This is an experimental data table of the present invention. DETAILED DESCRIPTION

[0052] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0053] See also Figure 1 The present invention provides a technical solution:

[0054] A salt-alkali resistant soil conditioner using mixed algae as raw materials: specifically composed of the following raw materials in parts by weight:

[0055] Mixed algae powder 50-80 parts;

[0056] Humic acid 10-30 parts;

[0057] 5-10 parts of microbial agent;

[0058] 1-5 portions of trace elements.

[0059] The mixed algae is a mixture of Spirulina, Chlorella and Nostoc in a weight ratio of 12:11:10.

[0060] The microbial agent is a mixture of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium in a weight ratio of 10:10:10.

[0061] The trace elements are a mixture of iron, zinc, boron and molybdenum in a weight ratio of 10:11:10:12.

[0062] In an embodiment of the present invention, a method for preparing a salt-alkali resistant soil conditioner using mixed algae as raw materials is as follows:

[0063] 1) Cultivation of mixed algae:

[0064] A. The formula of Spirulina culture medium is as follows:

[0065] a1. Preparation of culture medium: Sodium bicarbonate (16.8 g / L), dipotassium hydrogen phosphate (0.5 g / L), sodium nitrate (2.5 g / L), potassium sulfate (1.0 g / L), sodium chloride (1.0 g / L), magnesium sulfate heptahydrate (0.2 g / L), calcium chloride dihydrate (0.04 g / L), ferrous sulfate heptahydrate (0.01 g / L), and disodium ethylenediaminetetraacetic acid (0.08 g / L) were dissolved in distilled water in accordance with the formula ratio, the pH value was adjusted to 9.0, and the mixture was sterilized by high pressure sterilization in an autoclave, and cooled for use;

[0066] a2. Inoculation and culture: Take the spirulina algae seeds and inoculate them into a culture container filled with culture medium, with an inoculation amount of 10%-15%. The culture container can be a glass culture bottle, a plastic culture barrel, etc. The culture conditions are a temperature of 25-30℃, a light intensity of 2000-3000lx, and a light exposure time of 12-16h / d. During the culture process, stir the culture medium regularly to ensure that the spirulina cells can fully absorb nutrients and light;

[0067] a3. Collection and drying: When the concentration of Spirulina cells reaches 0.6-0.8 at a specific wavelength measured by a spectrophotometer, the cells are collected by centrifugation at 3000-4000 r / min for 10-15 min. The collected Spirulina cells are dried for 24-48 h using a freeze dryer at a drying temperature of -40°C to -50°C and a vacuum degree of 10-20 Pa. The dried Spirulina is crushed using a pulverizer and passed through a 100-mesh sieve to obtain Spirulina powder for later use.

[0068] B. Cultivation of Chlorella:

[0069] b1. Preparation of culture medium: Sodium nitrate (1.5 g / L), dipotassium hydrogen phosphate trihydrate (0.04 g / L), magnesium sulfate heptahydrate (0.075 g / L), calcium chloride dihydrate (0.036 g / L), citric acid (0.006 g / L), ammonium ferric citrate (0.006 g / L), disodium ethylenediaminetetraacetate (0.001 g / L), and sodium carbonate (0.02 g / L) were dissolved in distilled water in accordance with the formula ratio, the pH value was adjusted to 7.0, and the culture medium was sterilized by high pressure sterilization pot, and cooled for use;

[0070] b2. Inoculation and culture: Take the Chlorella algae and inoculate it into a glass culture bottle or plastic culture barrel filled with culture medium, with an inoculation amount of 5%-10%. Cultivate at a temperature of 25-30℃, a light intensity of 2000-3000lx, and a light duration of 12-16h / d. During the cultivation process, use a stirrer to regularly stir the culture solution;

[0071] b3. Collection and drying: When the concentration of Chlorella cells reaches 0.4-0.65 when the absorbance of the culture solution at a specific wavelength is measured by a spectrophotometer, the cells are collected by centrifugation at a speed of 3000-4000 r / min for 10-15 min. The collected Chlorella cells are dried for 24-48 h using a freeze dryer at a drying temperature of -40°C to -50°C and a vacuum degree of 10-20 Pa. The dried Chlorella is crushed using a pulverizer and passed through a 100-mesh sieve to obtain Chlorella powder for later use.

[0072] C. Nostoc culture:

[0073] c1. Culture medium preparation: same as that of Chlorella vulgaris;

[0074] c2. Inoculation and culture: Take the Nostoc algae seeds and inoculate them into a glass culture bottle or plastic culture bucket filled with culture medium, with an inoculation amount of 5%-10%. Cultivate at a temperature of 25-30℃, a light intensity of 2000-3000lx, and a light duration of 12-16h / d. During the cultivation process, use a stirrer to regularly stir the culture solution;

[0075] c3. Collection and drying: When the concentration of Nostoc cells reaches 0.4-0.65 when the absorbance of the culture solution at a specific wavelength is measured by a spectrophotometer, the cells are collected by centrifugation at a speed of 3000-4000r / min for 10-15min. The collected Nostoc cells are dried for 24-48h using a freeze dryer at a drying temperature of -40°C to -50°C and a vacuum degree of 10-20Pa. The dried Nostoc is crushed using a pulverizer and passed through a 100-mesh sieve to obtain Nostoc powder.

[0076] 2) Preparation of mixed algae powder: Spirulina powder, Chlorella powder and Nostoc powder are mixed in a weight ratio of 1:1:1 to obtain mixed algae powder.

[0077] 3) Crush the humic acid, pass it through an 80-mesh sieve, and crush it by mechanical crushing or air flow crushing. The mechanical crushing method can use a ball mill, a pulverizer and other equipment to crush the humic acid to the required particle size. The air flow crushing method uses high-speed airflow to crush the humic acid, which has the advantages of high crushing efficiency and uniform particle size.

[0078] 4) Crushing of microbial agents and trace elements:

[0079] D. Crushing of microbial agents: crush the microbial agents using a ball mill, a pulverizer or other equipment, and pass through a 100-mesh sieve, or use a freeze-crushing method to freeze the microbial agents at low temperatures and then crush them using a freeze-crushing machine, wherein the microbial agents are a mixture of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium, and the weight ratio thereof is 10:13:15.

[0080] F. Pulverizing trace elements: Pulverize the trace elements using a ball mill, a pulverizer, or other equipment, and pass through a 100-mesh sieve, or use an ultrasonic pulverization method to pulverize the trace elements to a desired particle size using an ultrasonic pulverizer. The trace elements are a mixture of iron, zinc, boron, and molybdenum, and the weight ratio is 10:10:10:10.

[0081] 5) Mixing preparation: the mixed algae powder obtained in step 1, the crushed humic acid powder, the crushed microbial agent powder and the trace element powder are mixed evenly by weight, and 5-10 parts by weight of urea are added to supplement the nitrogen element to obtain the salt-alkali resistant soil conditioner. The mixing method can adopt a mechanical stirring method, using a mixer to stir the various raw materials evenly; or adopt an airflow mixing method, using an airflow mixer to mix the various raw materials evenly.

[0082] In the embodiment of the present invention, the preparation method of the salt-alkali resistant soil conditioner using mixed algae as raw materials, the culture conditions of Spirulina, Chlorella and Nostoc are: temperature 25-30°C, light intensity 2000-3000lx, and light time 12-16h / d.

[0083] In the embodiment of the present invention, the preparation method of the salt-alkali resistant soil conditioner using mixed algae as raw materials comprises the step of drying by freeze drying.

[0084] In the embodiments of the present invention, the application of the salt-alkali resistant soil conditioner using mixed algae as raw materials includes the following:

[0085] S1. Application of base fertilizer: Before planting, apply the conditioner as base fertilizer to the soil. First, plow the soil to a depth of 20-30 cm to make the soil loose and breathable. Then evenly spread the conditioner on the surface of the plowed soil. Apply 120-200 kg per mu. Then use a rotary tiller or plow rake to fully mix the conditioner with the soil to ensure that the conditioner can be evenly distributed in the soil to improve the soil improvement effect.

[0086] S2. Topdressing: During the growth of plants, topdressing is applied;

[0087] Seedling stage: When the seedlings grow 2-3 true leaves, apply topdressing, evenly spread the conditioner on the soil surface, and then use small amounts of water for irrigation so that the conditioner can better penetrate into the soil. Apply 50-70 kg per mu during the seedling stage to promote the growth of the seedling root system and enhance the seedling's resistance to stress.

[0088] Growing period: When the plants enter the rapid growth period, topdressing can be carried out by furrow application. Dig furrows between the plant rows with a depth of 10-15 cm, apply the conditioner into the furrows, and then cover with soil. Apply 70-90 kg per mu during the growing period, which helps to improve soil fertility and meet the nutrient needs of plant growth.

[0089] Flowering and fruiting period: In order to ensure the normal flowering and fruiting of plants, nutrients need to be supplemented in time. At this time, topdressing should be applied again, 80-100kg per mu. You can choose to apply fertilizer by broadcasting or furrow application. Water in time after broadcasting. Cover the soil and water after furrow application to promote the reproductive growth of plants and improve fruit quality and yield;

[0090] S3. Foliar spraying: dilute the conditioner at a volume ratio of 1:500-1000 between the conditioner and water. Use a sprayer to evenly spray the diluted conditioner on both sides of the plant leaves until the leaves are moist but not dripping. Foliar spraying should be done in the morning or evening. Avoid spraying during high temperatures to avoid damaging the leaves. Foliar spraying allows plants to quickly absorb the nutrients in the conditioner, improve their stress resistance and photosynthesis efficiency. At the same time, foliar spraying can also reduce the impact of soil application on the environment and improve the utilization rate of fertilizers.

[0091] In the embodiment of the present invention, experiments verify that:

[0092] 1. Experimental materials:

[0093] (1) A saline-alkali land was selected as the experimental field. The salinity of the soil was 0.5%-1.0%, and the experimental crop was wheat.

[0094] (2) The salt-alkali resistant soil conditioner is a product prepared by the present invention.

[0095] (3) The following commercially available chemical conditioners and organic conditioners were used to test the products prepared by the present invention:

[0096] Ingredients of commercially available chemical conditioners:

[0097] Calcium sulfate: 30%-40%;

[0098] Ferrous sulfate: 20%-30%;

[0099] Monoammonium phosphate: 20%-30%;

[0100] Potassium sulfate: 10%-20%.

[0101] Ingredients ratio of machine conditioner:

[0102] Decomposed organic fertilizer: 40%-50%;

[0103] Microbial agents: 20%-30%;

[0104] Humic acid: 10%-20%.

[0105] 2. Experimental methods:

[0106] The experimental field was divided into three areas, and the salt-alkali resistant soil conditioner of the present invention, the commercially available chemical conditioner and the organic conditioner were applied respectively, with the area of ​​each area being 1 mu, and the application amount being 80 kg per mu. After application, conventional field management was carried out, and the growth of wheat was observed.

[0107] At different stages of wheat growth, soil samples and wheat plant samples were collected to measure soil salinity, fertility indicators and wheat growth indicators.

[0108] 3. Experimental results and analysis

[0109] (1) Changes in soil salinity

[0110] After the salt-alkali resistant soil conditioner of the present invention is applied, the salinity and alkalinity of the soil are significantly reduced.

[0111] (2) Changes in soil fertility indicators

[0112] After applying the salt-alkali resistant soil conditioner of the present invention, the soil fertility index is significantly improved, the content of nutrients such as organic matter, nitrogen, phosphorus, potassium, etc. in the soil is increased, and the pH value of the soil is also reduced. After applying commercially available chemical conditioners and organic conditioners, the soil fertility index is also improved, but the improvement is small.

[0113] (3) Changes in wheat growth indicators

[0114] After applying the salt-alkali resistant soil conditioner of the present invention, the growth index of wheat is obviously better than that of applying commercially available chemical conditioners and organic conditioners. The plant height, ear length, number of grains per ear, thousand-grain weight and other indicators of wheat are increased, and the yield of wheat is also improved. After applying commercially available chemical conditioners and organic conditioners, the growth index of wheat is also improved, but the improvement is small.

[0115] In summary, the conditioner uses mixed algae as the main raw material, has a wide source, low cost, and is pollution-free to the environment. The conditioner contains rich nutrients and bioactive substances, which can improve soil structure, increase soil fertility, reduce soil salinity, and promote crop growth. Experimental verification shows that the salt-alkali resistant soil conditioner of the present invention has a good application effect, can effectively improve saline-alkali land, and increase crop yield and quality. The salt-alkali resistant soil conditioner of the present invention has broad application prospects and market value.

[0116] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0117] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0118] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A salt-alkali resistant soil conditioner using mixed algae as raw materials, characterized in that: Specifically, it is composed of the following raw materials in parts by weight: Mixed algae powder 50-80 parts; Humic acid 10-30 parts; 5-10 parts of microbial agent; 1-5 portions of trace elements.

2. The salt-alkali resistant soil conditioner with mixed algae as raw materials according to claim 1, characterized in that: The mixed algae is a mixture of Spirulina, Chlorella and Nostoc, and the weight ratio thereof is 12:11:

10.

3. The salt-alkali resistant soil conditioner with mixed algae as raw materials according to claim 1, characterized in that: The microbial agent is a mixture of Bacillus subtilis, Bacillus licheniformis and Bacillus megaterium, and the weight ratio thereof is 10:10:

10.

4. The salt-alkali resistant soil conditioner with mixed algae as raw materials according to claim 1, characterized in that: The trace elements are a mixture of iron, zinc, boron and molybdenum in a weight ratio of 10:11:10:

12.

5. The method for preparing a salt-alkali resistant soil conditioner using mixed algae as raw materials according to any one of claims 1 to 4, characterized in that: 1) Cultivation of mixed algae: A. Spirulina culture: a1. Preparation of culture medium: Sodium bicarbonate (16.8 g / L), dipotassium hydrogen phosphate (0.5 g / L), sodium nitrate (2.5 g / L), potassium sulfate (1.0 g / L), sodium chloride (1.0 g / L), magnesium sulfate heptahydrate (0.2 g / L), calcium chloride dihydrate (0.04 g / L), ferrous sulfate heptahydrate (0.01 g / L), and disodium ethylenediaminetetraacetic acid (0.08 g / L) were dissolved in distilled water in accordance with the formula ratio, the pH value was adjusted to 9.0, and the mixture was sterilized by high pressure sterilization in an autoclave, and cooled for use; a2. Inoculation and culture: Take the Spirulina algae seeds and inoculate them into a glass culture bottle or plastic culture bucket filled with culture medium, with an inoculation amount of 10%-15%. Cultivate at a temperature of 25-30℃, a light intensity of 2000-3000lx, and a light duration of 12-16h / d. During the cultivation process, use a stirrer to regularly stir the culture solution; a3. Collection and drying: When the concentration of Spirulina cells reaches 0.6-0.8 at a specific wavelength measured by a spectrophotometer, the cells are collected by centrifugation at 3000-4000 r / min for 10-15 min. The collected Spirulina cells are dried for 24-48 h using a freeze dryer at a drying temperature of -40°C to -50°C and a vacuum degree of 10-20 Pa. The dried Spirulina is crushed using a pulverizer and passed through a 100-mesh sieve to obtain Spirulina powder for later use. B. Cultivation of Chlorella: b1. Preparation of culture medium: Sodium nitrate (1.5 g / L), dipotassium hydrogen phosphate trihydrate (0.04 g / L), magnesium sulfate heptahydrate (0.075 g / L), calcium chloride dihydrate (0.036 g / L), citric acid (0.006 g / L), ammonium ferric citrate (0.006 g / L), disodium ethylenediaminetetraacetate (0.001 g / L), and sodium carbonate (0.02 g / L) were dissolved in distilled water in accordance with the formula ratio, the pH value was adjusted to 7.0, and the culture medium was sterilized by high pressure sterilization pot, and cooled for use; b2. Inoculation and culture: Take the Chlorella algae and inoculate it into a glass culture bottle or plastic culture barrel filled with culture medium, with an inoculation amount of 5%-10%. Cultivate at a temperature of 25-30℃, a light intensity of 2000-3000lx, and a light duration of 12-16h / d. During the cultivation process, use a stirrer to regularly stir the culture solution; b3. Collection and drying: When the concentration of Chlorella cells reaches 0.4-0.65 when the absorbance of the culture solution at a specific wavelength is measured by a spectrophotometer, the cells are collected by centrifugation at a speed of 3000-4000 r / min for 10-15 min. The collected Chlorella cells are dried for 24-48 h using a freeze dryer at a drying temperature of -40°C to -50°C and a vacuum degree of 10-20 Pa. The dried Chlorella is crushed using a pulverizer and passed through a 100-mesh sieve to obtain Chlorella powder for later use. C. Nostoc culture: c1. Culture medium preparation: same as that of Chlorella vulgaris; c2. Inoculation and culture: Take the Nostoc algae seeds and inoculate them into a glass culture bottle or plastic culture bucket filled with culture medium, with an inoculation amount of 5%-10%. Cultivate at a temperature of 25-30℃, a light intensity of 2000-3000lx, and a light duration of 12-16h / d. During the cultivation process, use a stirrer to regularly stir the culture solution; c3. Collection and drying: When the concentration of Nostoc cells reaches 0.4-0.65 when the absorbance of the culture solution at a specific wavelength is measured by a spectrophotometer, the cells are collected by centrifugation at a speed of 3000-4000r / min for 10-15min. The collected Nostoc cells are dried for 24-48h using a freeze dryer at a drying temperature of -40°C to -50°C and a vacuum degree of 10-20Pa. The dried Nostoc is crushed using a pulverizer and passed through a 100-mesh sieve to obtain Nostoc powder. 2) Preparation of mixed algae powder: Spirulina powder, Chlorella powder and Nostoc powder are mixed in a weight ratio of 1:1:1 to obtain mixed algae powder. 3) Humic acid crushing: crush the humic acid using a ball mill, crusher or other equipment, and pass it through an 80-mesh sieve. Alternatively, use an airflow crushing method to crush the humic acid into the required particle size using an airflow crusher. 4) Crushing of microbial agents and trace elements: D. Crushing of microbial agents: Crushing the microbial agents using a ball mill, a pulverizer, or other equipment, and passing through a 100-mesh sieve. Alternatively, a freezing crushing method is used, where the microbial agents are frozen at low temperatures and then crushed using a freezing crusher. The microbial agents are a mixture of Bacillus subtilis, Bacillus licheniformis, and Bacillus megaterium, with a weight ratio of 10:13:

15. F. Pulverizing trace elements: Pulverize the trace elements using a ball mill, a pulverizer, or other equipment, and pass through a 100-mesh sieve, or use an ultrasonic pulverization method to pulverize the trace elements to a desired particle size using an ultrasonic pulverizer. The trace elements are a mixture of iron, zinc, boron, and molybdenum, and the weight ratio is 10:10:10:

10. 5) Mixing preparation: the mixed algae powder obtained in step 1, the crushed humic acid powder, the crushed microbial agent powder and the trace element powder are mixed evenly according to weight portions to obtain the salt-alkali resistant soil conditioner. The mixing method adopts a mechanical stirring method, using a mixer to mix the various raw materials evenly; or an air flow mixing method is adopted, using an air flow mixer to mix the various raw materials evenly.

6. The preparation method according to claim 5, characterized in that: In step 1), the culture conditions of Spirulina, Chlorella and Nostoc are: temperature 25-30° C., light intensity 2000-3000 lx, and illumination time 12-16 h / d.

7. The preparation method according to claim 5, characterized in that: In step 1), the drying is freeze-drying.

8. The use of a salt-alkali resistant soil conditioner using mixed algae as raw materials according to any one of claims 1 to 4, characterized in that: These include the following: S1. Application of base fertilizer: Before planting, apply the conditioner as base fertilizer to the soil and mix it thoroughly with the soil; S2. Topdressing: During the growth of plants, topdressing is applied; S3. Foliar spraying: dilute the conditioner and spray it on the leaves.

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