Dental pulp stem cell extraction device and extraction method thereof

Abstract

The invention discloses a dental pulp stem cell extraction device and an extraction method thereof, and relates to the technical field of dental pulp stem cell extraction.The dental pulp stem cell extraction device comprises a base plate, a tooth clamping device arranged on the base plate and a switching type tooth slitting mechanism located on the upper portion of the tooth clamping device; the switching type tooth slitting mechanism comprises a switching turntable located at the upper part of the rotary clamping part, and a polishing part, a washing part, a grooving part and a clamping and separating device which are arranged at the bottom of the switching turntable in an annular array, and the clamping and separating device is used for clamping grooves machined in teeth by the grooving part; and the teeth are separated into two halves under the driving of the rotary clamping component. When the dental pulp stem cell extraction device and the extraction method are used for extracting dental pulp stem cells, teeth can be separated so as to fully expose a dental pulp cavity, meanwhile, the teeth can be fully cleaned in the tooth separation process so as to prevent dental pulp from being polluted, chippings can be prevented from being generated in the tooth separation process, and the dental pulp stem cell extraction device can be used for extracting dental pulp stem cells. The dental pulp is prevented from being polluted.

Description

Technical Field

[0001] The present invention mainly relates to the technical field of dental pulp stem cell extraction, and specifically relates to a dental pulp stem cell extraction device and an extraction method thereof. Background Art

[0002] Dental pulp stem cells are a type of mesenchymal stem cells that exist in the pulp tissue inside the tooth. When extracting dental pulp stem cells, the tooth needs to be destroyed to expose the pulp cavity, the pulp is peeled off from the pulp cavity, and the pulp is processed by enzymatic hydrolysis to obtain dental pulp stem cells.

[0003] The dental pulp extraction device in the prior art includes a dental pulp extraction tool, a dental pulp stem cell extractor, a dental pulp transfer window, a dental pulp stem cell extraction tube, a dental pulp stem cell extraction tube carrier, a tooth body carrier, a motor, a high-efficiency filter, a tooth body clamp screw, a tooth body universal control component, a tooth body clamp, a tooth body clamp, a forward X-ray machine, a lateral X-ray machine, an extraction device body, a compressor cooling fan and a compressor. The prior art adopts a composite rotating clamping structure, which integrates cutting, clamping, fixing and separation functions, is suitable for the extraction needs of single and multiple tooth roots, can protect the dental pulp from damage during extraction, and the inner tooth saw blade can be replaced.

[0004] The existing technology can protect the dental pulp from loss during extraction, but it is not convenient for fully dividing and exposing the dental pulp cavity, and is not convenient for extracting the dental pulp tissue without residue. Summary of the invention

[0005] Based on this, the purpose of the present invention is to provide a dental pulp stem cell extraction device and an extraction method thereof to solve the technical problems raised in the above background technology.

[0006] To achieve the above-mentioned object, the present invention provides the following technical solutions: a dental pulp stem cell extraction device, comprising a base plate, a tooth clamping device arranged on the base plate, and a switchable tooth cutting mechanism located on the upper part of the tooth clamping device, wherein the tooth clamping device comprises a lifter arranged on the base plate, a lifting plate arranged at the execution end of the lifter, a plane mover arranged on the top of the lifting plate, and a rotating clamping component arranged at the execution end of the plane mover; The switchable tooth cutting mechanism comprises a switch turntable located on the upper part of the rotating clamping component, a grinding component, a flushing component, a grooving component and a clamping and separating device arranged in an annular array at the bottom of the switch turntable, wherein the clamping and separating device is used to clamp the groove processed on the tooth by the grooving component and separate the tooth into two halves under the drive of the rotating clamping component; The snap-on and separation device includes a track arranged at the bottom of the switching turntable, two second sliders slidably connected to the track and symmetrically arranged, two first springs symmetrically arranged at both ends of the inner wall of the track, and an automatic slot component arranged at one end of the bottom of the second slider and a magnetic linkage component at the other end.

[0007] Preferably, the rotating clamping component includes a base plate disposed at the execution end of the plane mover, two first sliders slidably connected to the top of the base plate and symmetrically arranged, a first motor disposed at the top of the base plate, a bidirectional screw disposed at the execution end of the first motor and connected to the screw nut of the first slider, a rotating rod rotatably connected to the top of the side wall of the first slider, a suction cup disposed at the end of the rotating rod, and a second motor disposed on one of the first sliders and used to drive the rotating rod to rotate. In this preferred embodiment, the teeth are stably clamped and rotated by rotating the clamping component.

[0008] Preferably, the grinding component includes an n-shaped frame arranged at the bottom of the switching turntable, a grinding roller rotatably connected to the n-shaped frame and arranged horizontally, and a driving motor arranged on the outer wall of the n-shaped frame and used to drive the grinding roller to rotate. In this preferred embodiment, the grinding of the tooth surface is achieved by the grinding component, which is convenient for cleaning the tooth surface and flattening the tooth surface, so as to reduce the possibility of waste chips generated when the teeth are separated.

[0009] Preferably, the flushing component includes a driving cylinder disposed on the top of the switching turntable and having an execution end passing through the switching turntable, a finger cylinder disposed at the execution end of the driving cylinder, two sealing boxes symmetrically disposed at the execution end of the finger cylinder, a water inlet pipe connected to one of the sealing boxes at one end, and a drain pipe connected to the other sealing box at one end. In this preferred embodiment, the flushing component facilitates cleaning of the tooth surface.

[0010] Preferably, the grooving component includes a power motor disposed on the top of the switching turntable and having an execution end passing through the switching turntable, an extension rod disposed at the execution end of the power motor, and a grooved ball head disposed at the end of the extension rod. In this preferred embodiment, the grooving component facilitates grooving of the tooth surface, so as to prepare in advance for the separation of the teeth.

[0011] Preferably, the automatic slotting component includes a crossbar disposed on the outer wall of the second slider, a first clamping block disposed at the bottom of the crossbar, vertical bars symmetrically disposed at both ends of the crossbar and hinged to the crossbar, a second clamping block disposed on the side wall of the vertical bar, a magnetic block disposed on the side of the vertical bar away from the second clamping block, an n-shaped bar connected to the top of the crossbar at the bottom, and two first electromagnetic blocks symmetrically disposed on both sides of the n-shaped bar and abutting against the magnetic block. In this preferred embodiment, the automatic slotting component is convenient for inserting into the annular groove opened on the tooth surface, so that teeth of different sizes can be separated conveniently.

[0012] Preferably, the magnetic linkage component includes a positioning box disposed on the outer wall of the second slider, a second electromagnetic block slidably connected to the inner wall of the positioning box at the top, a second spring disposed at the top of the inner wall of the positioning box, two extension plates symmetrically disposed on both sides of the positioning box, an arc-shaped slide rail disposed at the end of the extension plate, and a connecting rod with one end connected to the outer wall of the vertical rod and the other end slidably connected to the arc-shaped slide rail. In this preferred embodiment, the magnetic linkage component facilitates magnetic connection or separation with the execution end of the tooth clamping device.

[0013] Preferably, the lifter includes a plurality of guide sleeves arranged on the base plate, a guide column whose bottom is slidably connected to the inner wall of the guide sleeve and whose top is connected to the bottom of the lifting plate, a positioning sleeve arranged at the center position of the top of the base plate, a positioning rod whose bottom is slidably connected to the inner wall of the positioning sleeve and whose top is connected to the bottom of the lifting plate, a driving groove embedded in the bottom of the positioning rod, a first stepper motor arranged at the bottom of the base plate, and a driving screw arranged at the execution end of the first stepper motor and connected to the nut of the driving groove; The plane mover includes a first linear module disposed on the top of the lifting plate, and a second linear module disposed at the execution end of the first linear module and perpendicular to the first linear module, wherein the execution end of the second linear module is connected to the bottom of the base plate. In this preferred embodiment, the elevator is used to achieve stable lifting of the teeth to be processed, and the plane mover is used to achieve stable movement of the teeth to be processed on the plane.

[0014] Preferably, a second stepper motor is provided on the base plate, the execution end of the second stepper motor is connected to one end of the driving shaft, and the other end of the driving shaft passes through the lifting plate and is connected to the bottom of the switching turntable. In this preferred embodiment, the second stepper motor is used to realize the stable rotation of the switching turntable, so as to move the grinding part, the flushing part, the grooving part and the clamping and separating device to the working position.

[0015] According to the technical solution of the dental pulp stem cell extraction device described above, a method for extracting dental pulp stem cells is also provided, comprising the following steps: Step 1: X-ray the teeth; X-ray the teeth to obtain information about the pulp cavity of the teeth; Step 2: grinding the tooth; the tooth clamping device clamps the tooth, and after the clamping is completed, the grinding component grinds the outer surface of the tooth to obtain tooth 1; Step 3: Tooth grooving: the grooving component performs an annular grooving on the surface of tooth 1 according to the pulp cavity information to obtain tooth 2; Step 4: Cleaning the teeth: The flushing component flushes the surface of the second tooth to obtain the third tooth; Step 5, separation of teeth; the tooth clamping device applies a squeezing force to the tooth three and maintains it for a unit time. After completion, the clamping separation device clamps the annular groove on the tooth three and applies a separation force to the tooth through the tooth clamping device and maintains it for a unit time to complete a tooth separation process. The tooth separation process is repeated until the tooth three is separated into two halves to obtain two teeth four; Step 6: Extraction of dental pulp tissue: Use forceps to peel off the dental pulp tissue from two teeth and place it in sterile PBS; Step 7: Digestion of dental pulp tissue: mince the dental pulp tissue and mix it with the preheated digestive enzyme, and digest it in a water bath for a unit time to obtain a digestive solution. Step 8, preparation of cell suspension: adding an equal volume of complete culture medium to the digestion solution 1, and centrifuging after mixing evenly, removing the supernatant after centrifugation, and resuspending the cells in complete culture medium for dental pulp mesenchymal stem cells; Step nine: inoculation and culture; inoculate the resuspended cells into a culture dish and place it in an incubator for culture to complete the extraction of dental pulp stem cells.

[0016] In summary, the present invention mainly has the following beneficial effects: When extracting dental pulp stem cells, the dental pulp stem cell extraction device and extraction method of the present invention can separate the teeth to fully expose the dental pulp cavity. At the same time, the teeth can be fully cleaned during the tooth separation process to prevent the dental pulp from being contaminated, and debris can be avoided when the teeth are separated, thereby preventing the dental pulp from being contaminated. The device of the present invention realizes stable fixation and position adjustment of teeth through the tooth clamping device, so as to facilitate the separation and processing of teeth. The tooth clamping device realizes stable lifting and lowering of the teeth to be processed through the elevator, realizes stable movement of the teeth to be processed on the plane through the plane mover, and realizes stable clamping and rotation of the teeth through the rotating clamping component. The second stepper motor is used to realize the stable rotation of the switching turntable, so as to move the grinding component, the flushing component, the grooving component and the clamping separation device to the working position. The grinding component is used to grind the tooth surface, so as to clean the tooth surface and level the tooth surface at the same time, so as to reduce the possibility of waste chips when the teeth are separated. The flushing component is used to clean the tooth surface, and the grooving component is used to groove the tooth surface, so as to prepare in advance for the separation of the teeth. The stable separation of teeth is achieved through a snap-on separation device. The automatic snap-on component in the snap-on separation device facilitates the snapping into the annular groove opened on the surface of the tooth, making it convenient to separate teeth of different sizes. The magnetic linkage component facilitates magnetic connection or separation with the execution end of the tooth clamping device. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is an axonometric diagram of the overall structure of the device of the present invention; Figure 2 It is an exploded view of the overall structure of the device of the present invention; Figure 3 An exploded view of the tooth holding device structure of the present invention; Figure 4 It is an exploded view of the bottom structure of the switching turntable of the present invention; Figure 5 It is an exploded view of the structure of the clamping and separating device of the present invention; Figure 6 It is an exploded view of the structure of the magnetic attraction linkage component of the present invention; Figure 7 It is a cross-sectional view of the overall structure of the device of the present invention; Figure 8 A cross-sectional view of the switching turntable structure of the present invention; Fig. 9 The figure is a flow chart of the extraction method of the present invention.

[0018] Description of the drawings: 10, base plate; 20, tooth clamping device; 21, lifter; 211, guide sleeve; 212, guide column; 213, positioning sleeve; 214, positioning rod; 215, driving groove; 216, first stepper motor; 217, driving screw; 22, lifting plate; 23, plane mover; 231, first linear module; 232, second linear module; 24, rotating clamping component; 241, base plate; 242, first slider; 243, first motor; 244, bidirectional screw; 245, rotating rod; 246, suction cup; 247, second motor; 30, switching turntable; 301, second stepper motor; 302, driving shaft; 31, grinding component; 311, n-shaped frame; 312, grinding roller; 313, driving Motor; 32, flushing component; 321, drive cylinder; 322, finger cylinder; 323, sealing box; 324, water inlet pipe; 325, drain pipe; 33, grooving component; 331, power motor; 332, extension rod; 333, slotted ball head; 40, snap-on separation device; 41, track; 42, second slider; 43, first spring; 44, automatic slotting component; 441, cross bar; 4411, first clamping block; 442, vertical bar; 4421, second clamping block; 443, magnetic block; 444, n-shaped rod; 445, first electromagnetic block; 45, magnetic linkage component; 451, positioning box; 452, second electromagnetic block; 453, second spring; 454, extension plate; 455, arc-shaped slide rail; 456, connecting rod. DETAILED DESCRIPTION

[0019] The following will be combined with the accompanying drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.

[0020] The following describes an embodiment of the present invention based on its overall structure. Example

[0021] Please refer to the attached Figure 1 , 2As shown in Figures 3, 7, and 9, in a preferred embodiment of the present invention, a dental pulp stem cell extraction device includes a base plate 10, a tooth clamping device 20 disposed on the base plate 10, and a switchable tooth cutting mechanism located on the upper part of the tooth clamping device 20, wherein the tooth clamping device 20 includes a lifter 21 disposed on the base plate 10, a lifting plate 22 disposed at the execution end of the lifter 21, a plane mover 23 disposed on the top of the lifting plate 22, and a rotating clamp disposed at the execution end of the plane mover 23. The rotating clamping component 24 includes a base plate 241 disposed at the execution end of the plane mover 23, two first sliders 242 slidably connected to the top of the base plate 241 and symmetrically arranged, a first motor 243 disposed at the top of the base plate 241, a bidirectional screw rod 244 disposed at the execution end of the first motor 243 and connected to the nut of the first slider 242, a rotating rod 245 rotatably connected to the top of the side wall of the first slider 242, a suction cup 246 disposed at the end of the rotating rod 245, and a A second motor 247 on the first slider 242 and used to drive the rotating rod 245 to rotate, the lifter 21 includes a plurality of guide sleeves 211 arranged on the base plate 10, a guide column 212 whose bottom is slidably connected to the inner wall of the guide sleeve 211 and whose top is connected to the bottom of the lifting plate 22, a positioning sleeve 213 arranged at the center of the top of the base plate 10, a positioning rod 214 whose bottom is slidably connected to the inner wall of the positioning sleeve 213 and whose top is connected to the bottom of the lifting plate 22, and a driving groove embedded in the bottom of the positioning rod 214 215, a first stepper motor 216 arranged at the bottom of the base plate 10, and a driving screw rod 217 arranged at the execution end of the first stepper motor 216 and connected to the nut of the driving slot 215; the plane mover 23 includes a first linear module 231 arranged at the top of the lifting plate 22, and a second linear module 232 arranged at the execution end of the first linear module 231 and distributed perpendicularly to the first linear module 231, and the execution end of the second linear module 232 is connected to the bottom of the base plate 241.

[0022] It should be noted that, in the present embodiment, when extracting dental pulp stem cells, it is necessary to first perform an X-ray of the teeth, and the X-ray image information after the shooting is uploaded to the controller, and the controller obtains the dental pulp cavity information of the teeth after analysis. After the dental pulp cavity information is obtained, the teeth need to be polished, grooved, cleaned, and separated in sequence to separate the teeth so that the dental pulp cavity is fully exposed. After the dental pulp cavity is fully exposed, the dental pulp tissue is peeled off from the two halves of the tooth with tweezers and placed in sterile PBS, the dental pulp tissue is cut into pieces and mixed with the preheated digestive enzyme, and an equal volume of complete culture medium is added after a unit time of water bath digestion, and the mixture is evenly mixed and centrifuged, and the supernatant is removed after the centrifugation is completed, and the cells are resuspended with a complete culture medium for dental pulp mesenchymal stem cells, and the resuspended cells are inoculated into a culture dish, and placed in an incubator for culture to complete the extraction of dental pulp stem cells; Furthermore, when the tooth clamping device 20 clamps the tooth, the elevator 21 can drive the tooth to be lifted and lowered, the plane mover 23 can drive the tooth to be moved in a plane, and the rotating clamping component 24 can clamp the tooth and drive the tooth to rotate; Furthermore, when the lifter 21 is working, the first stepper motor 216 actuator end drives the driving screw 217 to rotate, and the driving screw 217 drives the positioning rod 214 to slide in the positioning sleeve 213 through the driving slot 215 connected to the nut, so as to drive the lifting plate 22 to rise and fall. During the lifting and falling process of the lifting plate 22, the guide column 212 slides in the guide sleeve 211 to increase the stability of the lifting plate 22 when it is lifted and lowered; Furthermore, when the plane mover 23 is working, the execution end of the first linear module 231 drives the second linear module 232 to move, and the execution end of the second linear module 232 drives the rotating clamping component 24 to move; Furthermore, when the rotating clamping component 24 is working, the executing end of the first motor 243 drives the bidirectional screw rod 244 to rotate, and the bidirectional screw rod 244 drives the two first sliders 242 to approach or separate from each other. When the two first sliders 242 approach each other, the teeth to be processed are pressed between the two suction cups 246 to clamp and fix the teeth. The executing end of the second motor 247 can drive the rotating rod 245 to rotate to rotate the teeth to be processed.

[0023] Please refer to the attached Figure 2 , 4As shown in Figures 7 and 8, in another preferred embodiment of the present invention, the switching tooth cutting mechanism includes a switching turntable 30 located at the upper part of the rotating clamping component 24, a grinding component 31, a flushing component 32, a grooving component 33 and a snap-on separation device 40 arranged in an annular array at the bottom of the switching turntable 30, the snap-on separation device 40 is used to snap-on the grooves machined on the teeth by the grooving component 33, and separate the teeth into two halves under the drive of the rotating clamping component 24; the grinding component 31 includes an n-shaped frame 311 arranged at the bottom of the switching turntable 30, a grinding roller 312 rotatably connected to the n-shaped frame 311 and arranged horizontally, and a driving motor 313 arranged on the outer wall of the n-shaped frame 311 and used to drive the grinding roller 312 to rotate, the flushing component 32 includes a grinding component 31 arranged at the top of the switching turntable 30 and the execution end passes through the cutting The driving cylinder 321 of the switching turntable 30, the finger cylinder 322 arranged at the execution end of the driving cylinder 321, the two sealing boxes 323 symmetrically arranged at the execution end of the finger cylinder 322, one end of which is connected to the water inlet pipe 324 of one of the sealing boxes 323, and the other end of which is connected to the drainage pipe 325 of the other sealing box 323, the grooving component 33 includes a power motor 331 arranged at the top of the switching turntable 30 and the execution end passing through the switching turntable 30, an extension rod 332 arranged at the execution end of the power motor 331, and a slotted ball head 333 arranged at the end of the extension rod 332, a second stepping motor 301 is arranged on the base plate 10, the execution end of the second stepping motor 301 is connected to one end of the driving shaft 302, and the other end of the driving shaft 302 passes through the lifting plate 22 and is connected to the bottom of the switching turntable 30.

[0024] It should be noted that, in this embodiment, the execution end of the second stepper motor 301 can drive the driving shaft 302 to rotate, the driving shaft 302 can drive the switching turntable 30 to rotate, the switching turntable 30 can move the grinding component 31 or the flushing component 32 or the grooving component 33 or the clamping and separating device 40 to the processing station to process the teeth. When the teeth are polished, the execution end of the driving motor 313 drives the grinding roller 312 to rotate through the gear, and the grinding roller 312 grinds the surface of the teeth; Furthermore, when the tooth is grooved, the execution end of the power motor 331 drives the extension rod 332 to rotate, and the extension rod 332 drives the grooved ball head 333 to rotate, and the grooved ball head 333 performs a groove process on the tooth surface; Furthermore, during the teeth cleaning process, after the actuator end of the driving cylinder 321 drives the finger cylinder 322 to descend to the specified position, the actuator end of the finger cylinder 322 drives the two sealing boxes 323 to approach each other to form a sealed cavity. The teeth to be processed are located in the sealed cavity. The water source system connected to the water inlet pipe 324 supplies water, and the negative pressure system connected to the drain pipe 325 performs negative pressure suction. Water flows through the water inlet pipe 324 into the sealing box 323 to rinse and clean the teeth, and the waste water after rinsing is discharged through the drain pipe 325.

[0025] Please refer to the attached Figure 2 , 5 As shown in Figures 6 and 7, in another preferred embodiment of the present invention, the engaging and separating device 40 includes a track 41 provided at the bottom of the switching turntable 30, two second sliders 42 slidably connected to the track 41 and symmetrically arranged, two first springs 43 symmetrically arranged at both ends of the inner wall of the track 41, and an automatic slot component 44 provided at one end of the bottom of the second slider 42 and a magnetic attraction linkage component 45 at the other end. The automatic slot component 44 includes a cross bar 441 provided on the outer wall of the second slider 42, a first clamping block 4411 provided at the bottom of the cross bar 441, vertical bars 442 symmetrically provided at both ends of the cross bar 441 and hinged to the cross bar 441, a second clamping block 4421 provided on the side wall of the vertical bar 442, and a magnetic attraction linkage component 45 provided at the other end of the vertical bar 44. The magnetic block 443 is far away from the second clamping block 4421, the n-shaped rod 444 connected to the top of the cross bar 441 at the bottom, and two first electromagnetic blocks 445 symmetrically arranged on both sides of the n-shaped rod 444 and abutting against the magnetic block 443. The magnetic attraction linkage component 45 includes a positioning box 451 arranged on the outer wall of the second sliding block 42, a second electromagnetic block 452 slidably connected to the inner wall of the positioning box 451 at the top, a second spring 453 arranged on the top of the inner wall of the positioning box 451, two extension plates 454 symmetrically arranged on both sides of the positioning box 451, an arc-shaped slide rail 455 arranged at the end of the extension plate 454, and a connecting rod 456 with one end connected to the outer wall of the vertical rod 442 and the other end slidably connected to the arc-shaped slide rail 455.

[0026] It should be noted that, in the present embodiment, during the tooth separation process, the tooth clamping device 20 applies a squeezing force to the tooth to be processed and maintains the squeezing force for a unit time. After completion, the clamping separation device 40 clamps the annular groove on the tooth to be processed and applies a separation force to the tooth to be processed through the tooth clamping device 20 and maintains the separation force for a unit time to complete a tooth separation process. The tooth separation process is repeated until the tooth to be processed is separated into two halves. When the first motor 243 drives the two first sliders 242 to separate from each other through the bidirectional screw rod 244, a separation force is applied to the teeth to be processed. When the first motor 243 drives the two first sliders 242 to approach each other through the bidirectional screw rod 244, a squeezing force is applied to the teeth to be processed. Furthermore, when the snap-on and separation device 40 is working, the first spring 43 gives an elastic thrust to the second slider 42, so that the two second sliders 42 fit each other in the initial state, and when the automatic slot component 44 engages the annular groove on the tooth, the tooth clamping device 20 drives the tooth to be processed to move until the first clamping block 4411 is engaged in the annular groove, and the first electromagnetic block 445 is energized to generate a repulsive force with opposite polarity to the magnetic block 443, and the repulsive force drives the vertical rod 442 to rotate around the end hinge point until the second clamping block 4421 is engaged in the annular groove to complete the snap-on work, and the tooth clamping device During the process of the clamping and positioning of 20, the second electromagnetic block 452 moves accordingly in the positioning box 451. After the clamping and positioning of the tooth clamping device 20 is completed, the second electromagnetic block 452 is electromagnetically attracted to the rotating rod 245. When the two first sliders 242 are separated from each other, the second electromagnetic block 452, the positioning box 451, the extension plate 454, the arc slide rail 455 and the connecting rod 456 are used to give a separation force to the end of the vertical rod 442, and the second electromagnetic block 452, the positioning box 451 and the second slider 42 are used to give a separation force to the cross bar 441, so that the force is even when the teeth to be processed are separated.

[0027] According to the above embodiments, a method for extracting dental pulp stem cells is also provided, comprising the following steps: Step 1: X-ray the teeth; X-ray the teeth to obtain information about the pulp cavity of the teeth; Step 2: grinding the tooth; the tooth clamping device 20 clamps the tooth, and after the clamping is completed, the grinding component 31 grinds the outer surface of the tooth to obtain tooth 1; Step 3: Tooth grooving: the grooving component 33 performs an annular grooving on the surface of tooth 1 according to the pulp cavity information to obtain tooth 2; Step 4: Cleaning of teeth: The rinsing component 32 rinses the surface of the second tooth to obtain the third tooth; Step 5, separation of teeth; the tooth clamping device 20 applies a squeezing force to the tooth three and maintains it for a unit time. After completion, the clamping separation device 40 clamps the annular groove on the tooth three and applies a separation force to the tooth through the tooth clamping device 20 and maintains it for a unit time to complete a tooth separation process. The tooth separation process is repeated until the tooth three is separated into two halves to obtain two teeth four; Step 6: Extraction of dental pulp tissue: Use forceps to peel off the dental pulp tissue from two teeth and place it in sterile PBS; Step 7: Digestion of dental pulp tissue: mince the dental pulp tissue and mix it with the preheated digestive enzyme, and digest it in a water bath for a unit time to obtain a digestive solution. Step 8, preparation of cell suspension: adding an equal volume of complete culture medium to the digestion solution 1, and centrifuging after mixing evenly, removing the supernatant after centrifugation, and resuspending the cells in complete culture medium for dental pulp mesenchymal stem cells; Step nine: inoculation and culture; inoculate the resuspended cells into a culture dish and place it in an incubator for culture to complete the extraction of dental pulp stem cells.

[0028] The working principle of the present invention is: The electrical components in the present invention are all operated by the controller. When extracting dental pulp stem cells, the teeth need to be X-rayed first, and the X-ray image information is uploaded to the controller. The controller analyzes and obtains the dental pulp cavity information of the teeth. After the dental pulp cavity information is obtained, the teeth need to be polished, grooved, cleaned, and separated in sequence to separate the teeth so that the dental pulp cavity is fully exposed. After the dental pulp cavity is fully exposed, the dental pulp tissue is peeled off from the two halves of the tooth with tweezers and placed in sterile PBS. The dental pulp tissue is cut into pieces and mixed with the preheated digestive enzyme. After a unit time of water bath digestion, an equal volume of complete culture medium is added, and the mixture is centrifuged after being evenly mixed. After centrifugation, the supernatant is removed, and the cells are resuspended in a complete culture medium for dental pulp mesenchymal stem cells. The resuspended cells are inoculated into a culture dish and placed in an incubator for culture to complete the extraction of dental pulp stem cells. When the tooth clamping device 20 clamps the tooth, the elevator 21 can drive the tooth to rise and fall, the plane mover 23 can drive the tooth to move in a plane, and the rotating clamping component 24 can clamp the tooth and drive the tooth to rotate; When the lifter 21 is working, the first stepper motor 216 drives the driving screw 217 to rotate, and the driving screw 217 drives the positioning rod 214 to slide in the positioning sleeve 213 through the driving slot 215 connected to the nut, so as to drive the lifting plate 22 to rise and fall. During the lifting and falling process of the lifting plate 22, the guide column 212 slides in the guide sleeve 211 to increase the stability of the lifting plate 22 when it is lifted and lowered; When the plane mover 23 is working, the execution end of the first linear module 231 drives the second linear module 232 to move, and the execution end of the second linear module 232 drives the rotating clamping component 24 to move; When the rotating clamping component 24 is working, the execution end of the first motor 243 drives the bidirectional screw rod 244 to rotate, and the bidirectional screw rod 244 drives the two first sliders 242 to move closer to or away from each other. When the two first sliders 242 move closer to each other, the tooth to be processed is pressed between the two suction cups 246 to clamp and fix the tooth. The execution end of the second motor 247 can drive the rotating rod 245 to rotate to rotate the tooth to be processed. The execution end of the second stepper motor 301 can drive the driving shaft 302 to rotate, and the driving shaft 302 can drive the switching turntable 30 to rotate. The switching turntable 30 can move the grinding component 31 or the flushing component 32 or the grooving component 33 or the clamping and separating device 40 to the processing station to process the teeth. When the teeth are polished, the execution end of the driving motor 313 drives the grinding roller 312 to rotate through the gear, and the grinding roller 312 grinds the surface of the teeth; When the tooth is slotted, the execution end of the power motor 331 drives the extension rod 332 to rotate, and the extension rod 332 drives the slotting ball head 333 to rotate, and the slotting ball head 333 performs slotting on the tooth surface; During the tooth cleaning process, after the actuator end of the driving cylinder 321 drives the finger cylinder 322 to descend to the specified position, the actuator end of the finger cylinder 322 drives the two sealing boxes 323 to approach each other to form a sealed cavity, and the teeth to be processed are located in the sealed cavity. The water source system connected to the water inlet pipe 324 supplies water flow, and the negative pressure system connected to the drain pipe 325 performs negative pressure suction. Water flows through the water inlet pipe 324 into the sealing box 323 to rinse and clean the teeth, and the waste water after rinsing is discharged through the drain pipe 325; During the tooth separation process, the tooth clamping device 20 applies a squeezing force to the tooth to be processed and maintains the squeezing force for a unit time. After the process is completed, the clamping separation device 40 clamps the annular groove on the tooth to be processed and applies a separation force to the tooth to be processed through the tooth clamping device 20 and maintains the separation force for a unit time to complete a tooth separation process. The tooth separation process is repeated until the tooth to be processed is separated into two halves. When the first motor 243 drives the two first sliders 242 to separate from each other through the bidirectional screw rod 244, a separation force is applied to the teeth to be processed. When the first motor 243 drives the two first sliders 242 to approach each other through the bidirectional screw rod 244, a squeezing force is applied to the teeth to be processed. When the clamping and separating device 40 is working, the first spring 43 gives an elastic thrust to the second slider 42, so that the two second sliders 42 fit each other in the initial state. When the automatic clamping groove component 44 clamps the annular groove on the tooth, the tooth clamping device 20 drives the tooth to be processed to move until the first clamping block 4411 is clamped into the annular groove, and the first electromagnetic block 445 is energized to generate a repulsive force with opposite polarity to the magnetic block 443, and the repulsive force drives the vertical rod 442 to rotate around the end hinge point until the second clamping block 4421 is clamped into the annular groove to complete the clamping work. The tooth clamping device 20 clamps During the connection and positioning process, the second electromagnetic block 452 moves accordingly in the positioning box 451. After the tooth clamping device 20 is connected and positioned, the second electromagnetic block 452 is electromagnetically attracted to the rotating rod 245. When the two first sliders 242 are separated from each other, the second electromagnetic block 452, the positioning box 451, the extension plate 454, the arc slide rail 455 and the connecting rod 456 are used to give a separation force to the end of the vertical rod 442, and the second electromagnetic block 452, the positioning box 451 and the second slider 42 are used to give a separation force to the cross bar 441, so that the force is even when the teeth to be processed are separated.

[0029] Although an embodiment of the present invention has been shown and described, this specific embodiment is merely an explanation of the present invention and is not a limitation of the invention. The specific features, structures, materials or characteristics described may be combined in a suitable manner in any one or more embodiments or examples. After reading this specification, those skilled in the art may make modifications, substitutions and variations to the embodiments without creative contributions as needed without departing from the principles and purpose of the present invention. However, as long as they are within the scope of the claims of the present invention, they are protected by patent law.

Claims

1. A dental pulp stem cell extraction device, comprising a base plate (10), a tooth clamping device (20) disposed on the base plate (10), and a switchable tooth cutting mechanism located on the upper part of the tooth clamping device (20), characterized in that: The tooth clamping device (20) comprises a lifter (21) arranged on the base plate (10), a lifting plate (22) arranged at the execution end of the lifter (21), a plane mover (23) arranged on the top of the lifting plate (22), and a rotating clamping component (24) arranged at the execution end of the plane mover (23); The switchable tooth cutting mechanism comprises a switchable turntable (30) located on the upper part of a rotating clamping component (24), a grinding component (31), a flushing component (32), a groove cutting component (33) and a clamping and separating device (40) arranged in an annular array at the bottom of the switchable turntable (30), wherein the clamping and separating device (40) is used to clamp the groove machined on the tooth by the groove cutting component (33) and separate the tooth into two halves under the drive of the rotating clamping component (24); The snap-on and release device (40) comprises a track (41) disposed at the bottom of the switching turntable (30), two second sliders (42) slidably connected to the track (41) and symmetrically arranged, two first springs (43) symmetrically arranged at two ends of the inner wall of the track (41), and an automatic snap-on component (44) disposed at one end of the bottom of the second slider (42) and a magnetic attraction linkage component (45) at the other end.

2. A dental pulp stem cell extraction device according to claim 1, characterized in that: The rotating clamping component (24) comprises a base plate (241) arranged at the execution end of the plane mover (23), two first sliders (242) slidably connected to the top of the base plate (241) and symmetrically arranged, a first motor (243) arranged at the top of the base plate (241), a bidirectional screw rod (244) arranged at the execution end of the first motor (243) and connected to the nut of the first slider (242), a rotating rod (245) rotatably connected to the top of the side wall of the first slider (242), a suction cup (246) arranged at the end of the rotating rod (245), and a second motor (247) arranged on one of the first sliders (242) and used to drive the rotating rod (245) to rotate.

3. The dental pulp stem cell extraction device according to claim 1, characterized in that: The grinding component (31) comprises an n-shaped frame (311) arranged at the bottom of the switching turntable (30), a grinding roller (312) rotatably connected to the n-shaped frame (311) and arranged horizontally, and a driving motor (313) arranged on the outer wall of the n-shaped frame (311) and used for driving the grinding roller (312) to rotate.

4. The dental pulp stem cell extraction device according to claim 1, characterized in that: The flushing component (32) comprises a driving cylinder (321) disposed at the top of the switching turntable (30) and having an execution end passing through the switching turntable (30), a finger cylinder (322) disposed at the execution end of the driving cylinder (321), two sealing boxes (323) symmetrically disposed at the execution end of the finger cylinder (322), a water inlet pipe (324) having one end connected to one of the sealing boxes (323), and a drainage pipe (325) having one end connected to the other of the sealing boxes (323).

5. The dental pulp stem cell extraction device according to claim 1, characterized in that: The groove cutting component (33) comprises a power motor (331) disposed on the top of the switching turntable (30) and having an execution end passing through the switching turntable (30), an extension rod (332) disposed on the execution end of the power motor (331), and a grooved ball head (333) disposed on the end of the extension rod (332).

6. The dental pulp stem cell extraction device according to claim 1, characterized in that: The automatic slot component (44) comprises a cross bar (441) arranged on the outer wall of the second sliding block (42), a first clamping block (4411) arranged at the bottom of the cross bar (441), a vertical bar (442) symmetrically arranged at both ends of the cross bar (441) and hinged to the cross bar (441), a second clamping block (4421) arranged on the side wall of the vertical bar (442), a magnetic block (443) arranged on the side of the vertical bar (442) away from the second clamping block (4421), an n-shaped bar (444) connected at the bottom to the top of the cross bar (441), and two first electromagnetic blocks (445) symmetrically arranged on both sides of the n-shaped bar (444) and abutting against the magnetic block (443).

7. The dental pulp stem cell extraction device according to claim 6, characterized in that: The magnetic attraction linkage component (45) comprises a positioning box (451) arranged on the outer wall of the second sliding block (42), a second electromagnetic block (452) slidably connected to the inner wall of the positioning box (451) at the top, a second spring (453) arranged at the top of the inner wall of the positioning box (451), two extension plates (454) symmetrically arranged on both sides of the positioning box (451), an arc-shaped slide rail (455) arranged at the end of the extension plate (454), and a connecting rod (456) having one end connected to the outer wall of the vertical rod (442) and the other end slidably connected to the arc-shaped slide rail (455).

8. The dental pulp stem cell extraction device according to claim 2, characterized in that: The lifter (21) comprises a plurality of guide sleeves (211) arranged on the base plate (10), a guide column (212) whose bottom is slidably connected to the inner wall of the guide sleeves (211) and whose top is connected to the bottom of the lift plate (22), a positioning sleeve (213) arranged at the center of the top of the base plate (10), a positioning rod (214) whose bottom is slidably connected to the inner wall of the positioning sleeve (213) and whose top is connected to the bottom of the lift plate (22), a driving groove (215) embedded in the bottom of the positioning rod (214), a first stepper motor (216) arranged at the bottom of the base plate (10), and a driving screw rod (217) arranged at the execution end of the first stepper motor (216) and connected to the nut of the driving groove (215); The plane mover (23) comprises a first linear module (231) arranged on the top of the lifting plate (22), and a second linear module (232) arranged at an execution end of the first linear module (231) and distributed perpendicularly to the first linear module (231), wherein the execution end of the second linear module (232) is connected to the bottom of the base plate (241).

9. The dental pulp stem cell extraction device according to claim 1, characterized in that: A second stepper motor (301) is provided on the base plate (10), the execution end of the second stepper motor (301) is connected to one end of a driving shaft (302), and the other end of the driving shaft (302) passes through the lifting plate (22) and is connected to the bottom of the switching turntable (30).

10. The extraction method of the dental pulp stem cell extraction device according to any one of claims 1 to 9, characterized in that: The following steps are involved: Step 1: X-ray the teeth; X-ray the teeth to obtain information about the pulp cavity of the teeth; Step 2: Grinding of teeth; The tooth clamping device (20) clamps the tooth, and after the clamping is completed, the grinding component (31) grinds the outer surface of the tooth to obtain tooth 1; Step 3: Tooth grooving; The groove cutting component (33) performs an annular groove on the surface of the tooth one according to the pulp cavity information to obtain the tooth two; Step 4: Cleaning of teeth; The flushing component (32) flushes the surface of tooth 2 to obtain tooth 3; Step 5: Separation of teeth; The tooth clamping device (20) applies a squeezing force to the tooth three and maintains the force for a unit time. After completion, the clamping separation device (40) clamps the annular groove on the tooth three and applies a separation force to the tooth through the tooth clamping device (20) and maintains the force for a unit time to complete a tooth separation process. The tooth separation process is repeated until the tooth three is separated into two halves to obtain two teeth four; Step 6: Extraction of dental pulp tissue: Use forceps to peel off the dental pulp tissue from two teeth and place it in sterile PBS; Step 7: Digestion of dental pulp tissue: mince the dental pulp tissue and mix it with the preheated digestive enzyme, and digest it in a water bath for a unit time to obtain a digestive solution. Step 8, preparation of cell suspension: adding an equal volume of complete culture medium to the digestion solution 1, and centrifuging after mixing evenly, removing the supernatant after centrifugation, and resuspending the cells in complete culture medium for dental pulp mesenchymal stem cells; Step nine: inoculation and culture; inoculate the resuspended cells into a culture dish and place it in an incubator for culture to complete the extraction of dental pulp stem cells.

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