A thermostatted cartridge having nucleic acid molecule amplification structure and methods thereof

Abstract

The present application relates to the technical fields of amplification constant temperature card box, and discloses a constant temperature card box with nucleic acid molecule amplification structure and a method thereof, which comprises a card box, the inside of the card box is hollow, one side of the top of the card box is provided with a magnet, the inside of the card box is provided with a stabilizing structure, both sides of the bottom of the card box are provided with constant temperature structures, and the stabilizing structure comprises a placing plate, which is larger than the slot in the top of the card box.The constant temperature card box with nucleic acid molecule amplification structure and the method thereof have a heat preservation shell, the card box is provided with a heat preservation shell that can surround the amplification test tube to perform heating, temperature reduction and cooling treatment, the heat preservation shell can not only perform monomer omnidirectional temperature control to make the temperature more accurate, but also can cooperate with the outer ring and the inner ring to perform balance control on the amplification test tube, so that the test tube is always in a vertical state inside when the card box shakes during movement, and the original solution does not shake, thereby avoiding aerosol pollution.

Description

Technical Field

[0001] This invention relates to the field of amplification isothermal cartridge technology, specifically to an isothermal cartridge with a nucleic acid molecule amplification structure and its method. Background Technology

[0002] In patent application CN211365618U, the following is described: a box body and a top cover. The top cover is positioned on top of the box body. A front cover is hinged to the bottom front of the box body. Multiple fixing parts and supporting parts are symmetrically formed at intervals from top to bottom on both sides of the box body. Each fixing part forms a slot with its adjacent supporting part below it. A buffer layer is provided on the inner surface of each slot. Multiple tube plates for placing reagent tubes are arranged at intervals from top to bottom within the box body. Each tube plate is inserted into corresponding slots on both sides. Each tube plate has multiple tube insertion holes, and each tube insertion hole is embedded with an elastic rubber ring. The front cover... The cover is placed on the front end of the box, with the rear end of the cover abutting against the front end of each tube sheet. The box is integrally injection molded from glass fiber reinforced PC composite plastic. Its advantages are: the integral injection molding of the box from glass fiber reinforced PC composite plastic provides high strength, making it less prone to deformation under external force, thus effectively supporting and protecting the reagent tubes inside; when the reagent kit is shaken, the elastic rubber rings sandwiched between each tube insertion hole and the corresponding reagent tube, as well as the buffer layer on the inner surface of the slot, effectively reduce the shaking effect, preventing collisions between the reagent tubes inside the box and thus providing better protection for the reagent tubes.

[0003] In the prior art, including the aforementioned patents, the cartridges used in PCR instruments cannot be effectively temperature controlled. Rapid or slow heating and cooling will affect the final experimental results. For example, water-based cartridges will cause excessive heat loss, and if the cartridge shakes after amplification, the liquid in the test tube will fluctuate greatly, leading to greater contact with air and aerosol contamination, which will further affect the accuracy of the experimental data. Summary of the Invention

[0004] The problem this invention aims to solve is that the heating and cooling of amplification cartridges cannot be ideally controlled during use, affecting the accuracy of experimental results and easily causing aerosol contamination.

[0005] To solve the above-mentioned technical problems, the technical solution of the present invention is: a thermostatic cartridge with a nucleic acid molecule amplification structure and a method thereof, comprising a cartridge, the interior of which is hollow, a magnet provided on one side of the top of the cartridge, a stabilizing structure provided inside the cartridge, and thermostatic structures provided on both sides of the bottom of the cartridge. The stabilizing structure includes a placement plate, the placement plate being larger than the slot at the top of the cartridge, and a plurality of square slots arranged in a rectangular array on the placement plate. An outer ring is rotatably provided between the two sides of the square slots, and an inner ring is rotatably provided between the front and back sides of the inner wall of the outer ring. An amplification test tube is provided inside the inner ring.

[0006] The bottom of the inner ring is provided with an insulation shell, which is open and its internal shape fits the external shape of the amplification tube. The inner wall of the insulation shell is hollow and a spiral tube is provided inside the inner wall of the insulation shell. Telescopic tubes are passed through the top of the spiral tube. The bottom of the inner wall of the card box is provided with a partition plate. The constant temperature structure includes a heater and a refrigerator. The bottom of the heater and the refrigerator are respectively fixedly connected to one side of the top of the partition plate. A water tank is fixedly provided on one side of the heating plate and the cooling plate. There are four water tanks in total. Every two water tanks form a group and are fixedly connected to each other by an interconnecting pipe. One side of the water tank is fixedly connected to one side of the inner wall of the card box, and the top of one side of the water tank is fixedly connected to the telescopic tube.

[0007] A cover plate is rotatably mounted on one side of the top of the card box. A handle is mounted on one side of the top of the cover plate, and the handle is compatible with a magnet. Several springs are mounted on the top of the cover plate, and a pressing plate is mounted on the top of each spring. Limiting blocks are mounted on the front and rear sides of one side of the top of the card box. Several soft pads are mounted on the top of the pressing plate. Several circular grooves are formed at the bottom of the cover plate. Several supports are mounted at the bottom of the pressing plate. The bottom of each support penetrates the cover plate and extends to the outside of the cover plate. A stabilizing plate is mounted at the bottom of each support, and the stabilizing plate is compatible with the circular grooves.

[0008] Preferably, both the heating machine and the cooling machine are fixedly connected to one side by a connecting pipe, the connecting pipe being L-shaped, and the top of the two connecting pipes are respectively provided with a heating plate and a cooling plate.

[0009] Preferably, cylinders are provided on both sides of the top of the partition plate, and pneumatic telescopic rods are provided on the front and rear sides of the bottom of the inner wall of the card box. The top of the pneumatic telescopic rods penetrates the water tank and extends into the interior of the water tank, and a sealing plate is provided on the top of the pneumatic telescopic rods.

[0010] Preferably, a rotating shaft is rotatably provided on one side of the inner wall of the water tank, and a baffle is fixedly provided on one side of the rotating shaft. The baffle is circular, and a secondary rod is rotatably provided on the rear side of one side of the baffle. A main rod is rotatably provided at the bottom of the secondary rod, and the bottom of the main rod is rotatably connected to one side of the top of the sealing plate.

[0011] Preferably, a cover is rotatably provided on one side of the top of the card box, and a handle is provided on one side of the top of the cover. The handle is adapted to a magnet. Several springs are provided on the top of the cover, and a pressing plate is provided on the top of the springs. Limiting blocks are provided on the front and rear sides of one side of the top of the card box.

[0012] S1: When using, put the stock solution to be amplified into the amplification tube, and then close the tube cap. Then put the amplification tube into the insulation shell, and then put the entire cartridge into the PCR device. After that, the machine will start operating the heating, cooling and deheating processes. When heating, start the heater first. The heater heats the heating plate through the connecting tube. Then the heating plate will conduct the temperature to the water tank, causing the temperature of the liquid in the water tank to rise.

[0013] S2: After the liquid inside the water tank reaches the specified temperature, the cylinder is activated. The cylinder adds air into the pneumatic telescopic rod, causing the pneumatic telescopic rod to extend upward. The pneumatic telescopic rod will drive the sealing plate to move upward. At the same time, the sealing plate will drive the main rod to move upward. The main rod controls the auxiliary rod to move upward, rotating the baffle with the connection point with the rotating shaft as the center to open it. After opening, the liquid in the water tank will enter the telescopic tube from the water tank and then flow along the telescopic tube into the spiral tube inside each heat preservation shell. The spiral tube is wrapped inside the heat preservation shell, which can provide all-round heat preservation of the amplification test tube.

[0014] S3: As more liquid is injected, the main rod will fold around the point of rotational connection with the auxiliary rod as the center, without affecting the upward movement of the sealing plate. When cooling is performed after the heating is completed, the cylinder can be controlled to recover gas so that the pneumatic telescopic rod can drive the sealing plate downward to draw the high-temperature liquid in the spiral tube back into the water tank. After it is drawn back, as the main rod and auxiliary rod are gradually straightened, the baffle rotates and closes again. When cooling is required after cooling is completed, the cylinder on the other side is started according to the above method to discharge the liquid cooled by the cooling plate from the water tank on the other side into the spiral tube for cooling.

[0015] S4: After the heating, cooling, and deheating steps are completed and the stock solution amplification is finished, remove the cartridge from the device. Shaking during removal will cause the stock solution to slosh, increasing contact with air and forming aerosol contamination, which will affect subsequent observation of the stock solution. When the cartridge is removed and shaken, the weight of the insulation shell will keep the amplification tube inside it in a vertical position. When subjected to longitudinal or lateral shaking force, the inner and outer rings at the top will rotate accordingly. While rotating, the insulation shell and amplification tube will not change their vertical position. In this way, the stock solution can still be kept stable under shaking, reducing the occurrence of aerosol contamination. The insulation shell can be shaken without affecting the normal heating, cooling, and deheating steps, achieving a good balance between the two. If the user does not need to keep it stable and needs to shake the stock solution manually, such as when adding liquid to ensure thorough mixing with the stock solution, the user only needs to hold the cartridge in their hand and press down on the pressing plate. The pressing plate will move the stabilizing plate downward through the bracket to fix the inner and outer rings.

[0016] Compared with the prior art, the technical solution of the present invention has the following advantages:

[0017] (1) The card box is equipped with a heat preservation shell that can surround the amplification tube for heating, cooling and cooling. In addition to the temperature control of the individual tubes in all directions to make the temperature more accurate, the heat preservation shell can also be used with the outer ring and inner ring to balance the amplification tubes. When the card box is moved and shaken, the amplification tubes are always in a vertical state and the original solution inside will not shake, avoiding aerosol pollution. The heat preservation shell controls the liquid outflow and outflow by moving the sealing plate up and down in the water tank, and controls the baffle to open and close by moving the baffle. The baffle is opened when liquid flows out and closed when liquid flows back. The heat preservation shell is individually wrapped for each amplification tube and has a certain temperature preservation effect to prevent temperature loss. This allows for more accurate temperature control and facilitates the amplification of the original solution.

[0018] (2) The heat preservation shell can achieve the balancing effect without affecting the liquid injection control temperature, and the balancing effect can also be manually controlled to maintain it. When it is not necessary to maintain the balance, if it is necessary to add a solution to the original solution and then stir it thoroughly, the outer and inner rings can be limited by the cooperation between the stabilizing plate and the outer and inner rings so that the outer and inner rings no longer rotate. This allows the amplification tube to be shaken so that the liquid inside can be fully mixed, increasing the overall controllability and adjustability of the device. The control method of the stabilizing plate is also simple and convenient, and it can be operated by pressing with a finger. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0020] Figure 2This is a schematic diagram of the internal structure of the card holder of the present invention;

[0021] Figure 3 This is a front view of the internal structure of the card box of the present invention;

[0022] Figure 4 This is a schematic diagram of the stable structure of the present invention;

[0023] Figure 5 This is a schematic diagram of the outer ring structure of the present invention;

[0024] Figure 6 This is a schematic diagram of the inner ring structure of the present invention;

[0025] Figure 7 This is an exploded view of the spiral tube structure of the present invention;

[0026] Figure 8 This is a schematic diagram of the isothermal structure of the present invention;

[0027] Figure 9 This is a schematic diagram of the sealing plate structure of the present invention;

[0028] Figure 10 This is a schematic diagram of the baffle structure of the present invention;

[0029] Figure 11 This is a schematic diagram of the movable cover structure of the present invention;

[0030] Figure 12 This is a schematic diagram of the stabilizing plate structure of the present invention;

[0031] Figure 13 This is a schematic diagram of the external structure of the card box of the present invention.

[0032] In the diagram: 1. Cover plate; 2. Pressing plate; 3. Soft pad; 4. Magnet; 5. Card box; 6. Stabilizing structure; 601. Telescopic tube; 602. Insulation shell; 603. Inner ring; 604. Outer ring; 605. Placement plate; 606. Amplification tube; 607. Spiral tube; 7. Constant temperature structure; 701. Water tank; 702. Refrigeration unit; 703. Connecting pipe; 704. Pneumatic telescopic rod; 705. Heating unit; 706. Cylinder; 707. Interconnecting pipe; 708. Heating plate; 709. Secondary rod; 710. Main rod; 711. Sealing plate; 712. Rotating shaft; 713. Baffle; 8. Divider plate; 9. Handle; 10. Spring; 11. Bracket; 12. Stabilizing plate; 13. Limiting block. Detailed Implementation

[0033] To make the objectives, technical solutions, and advantages of the embodiments of this disclosure clearer, the technical solutions of the embodiments of this disclosure will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this disclosure. All other embodiments obtained by those skilled in the art based on the described embodiments of this disclosure without creative effort are within the scope of protection of this disclosure.

[0034] Unless otherwise defined, the technical or scientific terms used in this disclosure shall have the ordinary meaning understood by one of ordinary skill in the art to which this disclosure pertains. The terms "comprising" or "including," and similar terms used in this disclosure, mean that an element or object preceding the term encompasses the elements or objects listed following the term and their equivalents, without excluding other elements or objects. Terms such as "connected" or "linked" are not limited to physical or mechanical connections, but may also include electrical connections, whether direct or indirect. Terms such as "upper," "lower," "left," and "right" are used only to indicate relative positional relationships; these relative positional relationships may change accordingly when the absolute position of the described objects changes.

[0035] like Figures 1 to 13 As shown, the present invention provides a thermostatic cartridge with a nucleic acid molecule amplification structure and its method, including a cartridge 5, the interior of the cartridge 5 is hollow, a magnet 4 is provided on one side of the top of the cartridge 5, a stabilizing structure 6 is provided inside the cartridge 5, and thermostatic structures 7 are provided on both sides of the bottom of the cartridge 5. The stabilizing structure 6 includes a placement plate 605, the placement plate 605 is larger than the slot at the top of the cartridge 5, and a plurality of square slots are provided in a rectangular array on the placement plate 605. An outer ring 604 is rotatably provided between the two sides of the square slots, and an inner ring 603 is rotatably provided between the front and back sides of the inner wall of the outer ring 604. An amplification test tube 606 is provided inside the inner ring 603.

[0036] The bottom of the inner ring 603 is provided with a heat insulation shell 602. The heat insulation shell 602 is open and its internal shape fits the external shape of the amplification test tube 606. The inner wall of the heat insulation shell 602 is hollow. A spiral tube 607 is provided inside the inner wall of the heat insulation shell 602. Telescopic tubes 601 are passed through the top of the spiral tube 607. The bottom of the inner wall of the card box 5 is provided with a partition plate 8. The constant temperature structure 7 includes a heater 705 and a refrigerator 702. The bottom of the heater 705 and the refrigerator 702 are respectively fixedly connected to one side of the top of the partition plate 8. A water tank 701 is fixedly provided on one side of the heating plate 708 and the cooling plate. There are four water tanks 701. Every two water tanks 701 form a group and are fixedly connected to each other on opposite sides by an interconnecting pipe 707. One side of the water tank 701 is fixedly connected to one side of the inner wall of the card box 5. The top of one side of the water tank 701 is fixedly connected to the telescopic tube 601.

[0037] A cover plate 1 is rotatably mounted on one side of the top of the card box 5. A handle 9 is mounted on one side of the top of the cover plate 1. The handle 9 is compatible with the magnet 4. Several springs 10 are mounted on the top of the cover plate 1. A pressing plate 2 is mounted on the top of the springs 10. Limiting blocks 13 are mounted on the front and rear sides of one side of the top of the card box 5. Several soft pads 3 are mounted on the top of the pressing plate 2. Several circular grooves are opened at the bottom of the cover plate 1. Several brackets 11 are mounted at the bottom of the pressing plate 2. The bottom of the brackets 11 penetrates the cover plate 1 and extends to the outside of the cover plate 1. A stabilizing plate 12 is mounted at the bottom of the brackets 11. The stabilizing plate 12 is compatible with the circular grooves.

[0038] Both the heating unit 705 and the cooling unit 702 have a fixed connection pipe 703 on one side. The connection pipe 703 is L-shaped, and the top of the two connection pipes 703 is respectively provided with a heating plate 708 and a cooling plate.

[0039] Cylinders 706 are provided on both sides of the top of the partition plate 8. Pneumatic telescopic rods 704 are provided on the front and rear sides of the bottom of the inner wall of the card box 5. The top of the pneumatic telescopic rod 704 passes through the water tank 701 and extends into the interior of the water tank 701. A sealing plate 711 is provided on the top of the pneumatic telescopic rod 704.

[0040] A rotating shaft 712 is rotatably provided on one side of the inner wall of the water tank 701. A baffle 713 is fixedly provided on one side of the rotating shaft 712. The baffle 713 is circular. A secondary rod 709 is rotatably provided on the rear side of one side of the baffle 713. A main rod 710 is rotatably provided at the bottom of the secondary rod 709. The bottom of the main rod 710 is rotatably connected to one side of the top of the sealing plate 711.

[0041] S1: When using, put the stock solution to be amplified into the amplification tube 606, and after putting it in, close the tube cap. Then put the amplification tube 606 into the insulation shell 602. After putting it in, put the entire cartridge 5 into the PCR device. After putting it in, the machine will start operating the heating, cooling and deheating. When heating, first start the heater 705. The heater 705 heats the heating plate 708 through the connecting tube 703. Then the heating plate 708 will conduct the temperature to the water tank 701, causing the temperature of the liquid in the water tank 701 to rise.

[0042] S2: After the liquid inside the water tank 701 reaches the specified temperature, the cylinder 706 is activated. The cylinder 706 adds air into the pneumatic telescopic rod 704, causing the pneumatic telescopic rod 704 to extend upward. The pneumatic telescopic rod 704 drives the sealing plate 711 to move upward. At the same time, the sealing plate 711 moves upward, which in turn drives the main rod 710 to move upward. The main rod 710 controls the auxiliary rod 709 to move upward, causing the baffle 713 to rotate and open with the rotation connection point with the rotating shaft 712 as the center. After opening, the liquid in the water tank 701 will enter the telescopic tube 601 from the water tank 701 and then flow along the telescopic tube 601 to the spiral tube 607 inside each insulation shell 602. The spiral tube 607 is wrapped inside the insulation shell 602, which can provide all-round wrapping and heating of the amplification test tube 606.

[0043] S3: As more liquid is injected, the main rod 710 will fold around the rotating connection with the auxiliary rod 709 as the center, without affecting the upward movement of the sealing plate 711. When cooling is performed after the heating is completed, the cylinder 706 can be controlled to recover gas so that the pneumatic telescopic rod 704 can drive the sealing plate 711 to move downward and draw the high-temperature liquid in the spiral tube 607 back into the water tank 701. After being drawn back, as the main rod 710 and the auxiliary rod 709 are gradually straightened, the baffle 713 rotates and closes again. When cooling is required after cooling is completed, the cylinder 706 on the other side is started according to the above method to discharge the liquid cooled by the cooling plate from the water tank 701 on the other side into the spiral tube 607 for cooling.

[0044] S4: After the heating, cooling, and deheating steps are completed and the stock solution amplification is finished, remove cartridge 5 from the device. Shaking during removal will cause the stock solution to slosh, increasing contact with air and creating aerosol contamination that will affect subsequent observation. When cartridge 5 is removed and shaken, the weight of the insulation shell 602 will keep the amplification tube 606 inside upright. When subjected to longitudinal or lateral shaking forces, the inner ring 603 and outer ring 604 at the top will rotate accordingly. During this rotation, the insulation shell 602 and the amplification tube 606 will not change their vertical position. This design ensures that the original liquid remains stable even when shaken, reducing aerosol contamination. The insulation shell 602 can be shaken without affecting its normal heating, cooling, and de-heating processes, achieving a good balance between the two. If the user does not need to maintain a stable state and needs to manually shake the original liquid to allow it to slosh around, such as when adding liquid to ensure it is fully mixed with the original liquid, the user simply needs to hold the card box 5 in their hand and press down on the pressing plate 2. The pressing plate 2 will then move the stabilizing plate 12 downwards via the bracket 11 to fix the inner ring 603 and the outer ring 604.

[0045] The working principle and usage process of this invention are as follows: During use, the stock solution to be amplified is placed into the amplification tube 606. After placement, the tube cap is closed, and then the amplification tube 606 is placed into the insulation shell 602. After placement, the entire cartridge 5 is placed into the PCR device. Once placed inside, the machine begins its heating, cooling, and de-heating processes. During heating, the heating unit 705 is activated first. The heating unit 705 heats the heating plate 708 through the connecting pipe 703. Subsequently, the heating plate 708 conducts heat to the water tank 701, raising the temperature of the liquid inside. Once the liquid inside the water tank 701 reaches the specified temperature, the cylinder 706 is activated. The cylinder 706 adds air to the pneumatic telescopic rod 704, causing the pneumatic telescopic rod 704 to extend upwards. The telescopic rod 704 moves the sealing plate 711 upwards. Simultaneously, the upward movement of the sealing plate 711 moves the main rod 710 upwards. The main rod 710 controls the secondary rod 709 to move upwards, causing the baffle 713 to rotate and open around its connection point with the rotating shaft 712. After opening, the liquid in the water tank 701 enters the telescopic tube 601 and flows along it to the spiral tube 607 inside each insulation shell 602. The spiral tube 607, encased inside the insulation shell 602, provides comprehensive heating to the amplification tube 606. As more liquid is injected, the main rod 710, under continuous pressure, folds around its connection point with the secondary rod 709 without affecting the upward movement of the sealing plate 711. After heating, a cooling process is performed. The cylinder 706 can be controlled to recover gas, causing the pneumatic telescopic rod 704 to move the sealing plate 711 downwards, drawing the high-temperature liquid in the spiral tube 607 back into the water tank 701. After being drawn back, as the main rod 710 and the auxiliary rod 709 are gradually straightened, the baffle 713 rotates and closes again. When cooling is required after the cooling process is complete, the other cylinder 706 is activated according to the above method to discharge the liquid cooled by the cooling plate from the other water tank 701 into the spiral tube 607 for cooling. After the heating, cooling, and de-heating steps are completed, the original liquid amplification is completed, and the cartridge 5 is removed from the device. If shaking occurs during removal, it will cause the original liquid to slosh, increasing contact with air and forming aerosol contamination, affecting the subsequent observation of the original liquid. During shaking, the weight of the insulation shell 602 keeps the amplification tube 606 inside upright. When subjected to longitudinal or lateral shaking forces, the inner ring 603 and outer ring 604 at the top will rotate accordingly. While rotating, the insulation shell 602 and the amplification tube 606 do not change their vertical position. This ensures the stock solution remains stable and reduces aerosol contamination even under shaking. The insulation shell 602 can be shaken without affecting its normal heating, cooling, and de-heating processes, achieving a good balance. If the user does not require it to remain stable and needs to manually shake the stock solution, and if adding liquid requires thorough mixing with the stock solution, the user simply needs to hold the cartridge 5 in their hand and press down on the pressure plate 2.The pressing plate 2, through the bracket 11, moves the stabilizing plate 12 downwards, fixing the inner ring 603 and outer ring 604 so that they no longer act as a stabilizer. This allows the stock solution in the amplification tube 606 to be shaken.

[0046] The above embodiments are merely exemplary embodiments of the present invention and are not intended to limit the present invention. The scope of protection of the present invention is defined by the claims. Those skilled in the art can make various modifications or equivalent substitutions to the present invention within its spirit and scope of protection, and such modifications or equivalent substitutions should also be considered to fall within the scope of protection of the present invention.

Claims

1. A thermostatic cartridge with a nucleic acid molecule amplification structure, comprising a cartridge (5), characterized in that: The card box (5) is hollow inside. A magnet (4) is provided on one side of the top of the card box (5). A stabilizing structure (6) is provided inside the card box (5). A constant temperature structure (7) is provided on both sides of the bottom of the card box (5). The stabilizing structure (6) includes a placement plate (605). The placement plate (605) is larger than the slot at the top of the card box (5). Several square slots are provided in a rectangular array on the placement plate (605). An outer ring (604) is rotatably provided between the two sides of the square slots. An inner ring (603) is rotatably provided between the front and back sides of the inner wall of the outer ring (604). An amplification test tube (606) is provided inside the inner ring (603). The bottom of the inner ring (603) is provided with a heat-insulating shell (602). The heat-insulating shell (602) is open and its internal shape fits the external shape of the amplification tube (606). The inner wall of the heat-insulating shell (602) is hollow. A spiral tube (607) is provided inside the inner wall of the heat-insulating shell (602). Telescopic tubes (601) are passed through the top of the spiral tube (607) around its perimeter. A partition plate (8) is provided at the bottom of the inner wall of the card box (5). The constant temperature structure (7) includes a heater (705) and a refrigerator (702). The bottom of the heating machine (705) and the cooling machine (702) are fixedly connected to one side of the top of the partition plate (8). A water tank (701) is fixedly provided on one side of the heating plate (708) and the cooling plate. There are four water tanks (701). Each pair of water tanks (701) is a group and an interconnecting pipe (707) is fixedly connected between opposite sides. One side of the water tank (701) is fixedly connected to one side of the inner wall of the card box (5). The top of one side of the water tank (701) is fixedly connected to the telescopic pipe (601). The card box (5) has a cover plate (1) on one side of its top. The cover plate (1) has a handle (9) on one side of its top. The handle (9) is compatible with the magnet (4). The cover plate (1) has several springs (10) on its top. The springs (10) have a pressing plate (2) on their top. The front and rear sides of the top of the card box (5) have limiting blocks (13). The pressing plate (2) has several soft pads (3) on its top. The cover plate (1) has several circular grooves at its bottom. The pressing plate (2) has several brackets (11) at its bottom. The brackets (11) penetrate the cover plate (1) and extend to the outside of the cover plate (1). The brackets (11) have a stabilizing plate (12) at their bottom. The stabilizing plate (12) is compatible with the circular grooves.

2. The isothermal cartridge with a nucleic acid molecule amplification structure according to claim 1, characterized in that: The heating machine (705) and the cooling machine (702) are both fixedly connected to one side by a connecting pipe (703). The connecting pipe (703) is L-shaped, and the top of the two connecting pipes (703) is respectively provided with a heating plate (708) and a cooling plate.

3. The isothermal cartridge with a nucleic acid molecule amplification structure according to claim 2, characterized in that: The top of the partition plate (8) is also provided with cylinders (706) on both sides. The front and rear sides of the bottom of the inner wall of the card box (5) are provided with pneumatic telescopic rods (704). The top of the pneumatic telescopic rod (704) penetrates the water tank (701) and extends into the interior of the water tank (701). The top of the pneumatic telescopic rod (704) is provided with a sealing plate (711).

4. The isothermal cartridge with a nucleic acid molecule amplification structure according to claim 3, characterized in that: A rotating shaft (712) is rotatably provided on one side of the inner wall of the water tank (701). A baffle (713) is fixedly provided on one side of the rotating shaft (712). The baffle (713) is circular. A secondary rod (709) is rotatably provided on the rear side of one side of the baffle (713). A main rod (710) is rotatably provided at the bottom of the secondary rod (709). The bottom of the main rod (710) is rotatably connected to one side of the top of the sealing plate (711).

5. A method of using a thermostatic cartridge with a nucleic acid molecule amplification structure, for use in the thermostatic cartridge with a nucleic acid molecule amplification structure as described in claim 4, characterized in that, Includes the following steps: S1: When using, put the stock solution to be amplified into the amplification tube (606), put it in and close the tube cap, then put the amplification tube (606) into the insulation shell (602), and then put the entire card box (5) into the PCR device. After putting it in, the machine starts to operate for heating, cooling and de-heating. When heating, start the heater (705) first. The heater (705) heats the heating plate (708) through the connecting tube (703). Then the heating plate (708) will conduct the temperature to the water tank (701) to raise the temperature of the liquid in the water tank (701). S2: After the liquid inside the water tank (701) reaches the specified temperature, the cylinder (706) is activated. The cylinder (706) adds air into the pneumatic telescopic rod (704) to extend the pneumatic telescopic rod (704) upward. The pneumatic telescopic rod (704) will drive the sealing plate (711) to move upward. At the same time, the sealing plate (711) will drive the main rod (710) to move upward. The main rod (710) controls the auxiliary rod (709) to move upward and rotate the baffle (713) with the rotating connection with the rotating shaft (712) as the center to open. After opening, the liquid in the water tank (701) will enter the telescopic tube (601) from the water tank (701) and then flow along the telescopic tube (601) to the spiral tube (607) inside each heat preservation shell (602). The spiral tube (607) is wrapped in the heat preservation shell (602) to provide all-round heating to the amplification test tube (606). S3: As more liquid is injected, the main rod (710) will be continuously pressurized and will fold around the rotating connection with the auxiliary rod (709) without affecting the upward movement of the sealing plate (711). When the cooling process is carried out after the heating is completed, the control cylinder (706) recovers the gas and the pneumatic telescopic rod (704) drives the sealing plate (711) to move downward, so that the high temperature liquid in the spiral tube (607) is drawn back into the water tank (701). After being drawn back, as the main rod (710) and the auxiliary rod (709) are gradually straightened, the baffle (713) rotates and closes again. When the cooling process is required after the cooling is completed, the other cylinder (706) is started according to the above method to discharge the liquid cooled by the cooling plate from the other water tank (701) into the spiral tube (607) for cooling. S4: After the heating, cooling and deheating steps are completed, the cartridge (5) is removed from the device after the stock solution amplification is finished. If shaking occurs during removal, it will cause the stock solution to slosh and increase contact with air, forming aerosol pollution and affecting the subsequent observation of the stock solution. When the cartridge (5) is removed and shaken, the weight of the heat preservation shell (602) will cause the amplification tube (606) inside to always be in a vertical position. When subjected to longitudinal or lateral shaking force, the inner ring (603) and outer ring (604) at the top will rotate accordingly. At the same time as the rotation, the heat preservation shell (602) and the amplification tube (606) will not change their vertical position. In this way, the original liquid remains stable even when shaken, reducing the occurrence of aerosol pollution. The heat preservation shell (602) can be shaken without affecting its normal heating, cooling and de-heating steps, achieving a better balance between the two. If the user does not need it to remain stable and needs to shake it manually to make the original liquid slosh, such as adding liquid to make it fully mix with the original liquid, the user only needs to hold the card box (5) in their hand and press down on the pressing plate (2). The pressing plate (2) will drive the stabilizing plate (12) to move down through the bracket (11) to fix the inner ring (603) and the outer ring (604).

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