Immersion shaking device for producing bioactive peptide by proteolysis of royal jelly and application of immersion shaking device

Abstract

The invention discloses a soaking and shaking device for producing bioactive peptide through royal jelly proteolysis. Comprising a base, a shaking assembly on the base is connected with a soaking and shaking box, and the shaking assembly is used for driving the soaking and shaking box to shake slowly, so that bioactive peptide obtained after royal jelly protease hydrolysis can be subjected to dust removal in the soaking and shaking process, or royal jelly is continuously subjected to hydrolysis treatment through royal jelly protease; the rocking assembly comprises a supporting mechanism and a power mechanism; the soaking and shaking box body is shaken through the shaking assembly, the shaking assembly comprises a supporting mechanism and a power mechanism, the soaking and shaking box body is supported through the supporting mechanism, and the power mechanism is pushed through a servo electric cylinder through a first crank rod and a second crank rod; and the soaking and shaking box body can be shaken according to the bending degree of the first crank rod and the second crank rod.

Description

Technical Field

[0001] The invention belongs to the technical field of biological medicines, and in particular relates to an immersion and shaking device for producing biologically active peptides by enzymatic hydrolysis of royal jelly and its application. Background Art

[0002] Royal jelly, also known as royal jelly, bee milk, and royal jelly, is the secretion of the pharyngeal glands of young worker bees that cultivate larvae in the beehive. It is the food for the larvae that will become queen bees. Royal jelly is high in protein and contains vitamin B and acetylcholine. Royal jelly cannot be taken with boiled water or tea, and should be stored at low temperatures. Royal jelly contains 62.5%-70% water, 1%-14.5% protein, 8.3%-15% sugars, 6% fatty acids, and 0.82%-1.5% trace elements. In addition, there is a certain amount of unknown substances. Royal jelly contains various amino acids and rich vitamins necessary for the human body, as well as inorganic salts, organic salts, enzymes, hormones and other biologically active substances. Royal jelly contains a variety of biologically active peptides and has high medicinal properties. However, various biologically active peptides on the market still have various problems when they are prepared.

[0003] When the bioactive peptides are hydrolyzed, the royal jelly needs to be hydrolyzed for a long time. During the hydrolysis, the royal jelly needs to be shaken and kept warm to improve the hydrolysis of the royal jelly and obtain a variety of bioactive peptides in the royal jelly, so as to facilitate the subsequent acquisition of the required bioactive peptides. However, the existing equipment cannot effectively achieve support and fixation when in use, and cannot effectively achieve slow shaking and other problems. For this reason, we propose an immersion and shaking device for producing bioactive peptides by enzymatic hydrolysis of royal jelly and its application. Summary of the invention

[0004] The purpose of the present invention is to provide a shaking device for producing bioactive peptides by enzymatic hydrolysis of royal jelly and its application, so as to solve the problems raised in the above-mentioned background technology.

[0005] To achieve the above-mentioned purpose, the present invention provides the following technical scheme: a shaking device for producing bioactive peptides by hydrolyzing royal jelly protease, comprising a base, a shaking assembly on the base being connected to a shaking box, the shaking assembly being used to drive the shaking box to shake slowly, so that the bioactive peptides hydrolyzed by royal jelly protease can be dispersed during the shaking process, or the royal jelly protease can continuously hydrolyze the royal jelly;

[0006] The shaking assembly includes a supporting mechanism and a power mechanism, wherein first supporting plates are welded on both sides of the middle part of the base, a first supporting rod is movably installed between the first supporting plates on both sides, a main supporting block is movably installed on the first supporting rod, and the main supporting block is fixedly installed in the middle of the bottom of the immersion and shaking box body, second supporting plates are fixedly installed on both sides of one end of the base, a second supporting rod is fixedly installed between the second supporting plates on both sides, two first crank rods are movably installed on the second supporting rod, and the ends of the two first crank rods are connected to one end of the immersion and shaking box body through auxiliary supporting blocks;

[0007] The power mechanism includes a third support plate fixed on both sides of the other end of the base, a third support rod is fixedly installed between the two third support plates, a second crank rod is movably connected to the third support rod, an end of the second crank rod is movably connected to the third crank rod through a second movable shaft, and an end of the third crank rod is connected to the other end of the immersion and shaking box through a bonding plate;

[0008] Fourth support plates are welded on both sides of the middle of the base, a servo electric cylinder is movably installed between the two fourth support plates, a push-pull block is fixedly connected to the output end of the servo electric cylinder, and the push-pull block is movably connected to the second movable shaft.

[0009] Preferably, an adjustment slot is opened at one end of the base, and the two fourth support plates are respectively welded on both sides of the adjustment slot, and the servo electric cylinder is movable inside the adjustment slot.

[0010] Preferably, a cover plate is movably mounted on the upper portion of the immersion and shaking box body through a hinge joint, a handle is fixedly mounted on one side of the cover plate, one side of the cover plate is connected to a feed pipe, and a second valve is fixedly mounted on the feed pipe.

[0011] Preferably, one end of the immersion and shaking box is fixedly connected to a discharge pipe, and a first valve is fixedly installed on the discharge pipe.

[0012] Preferably, both sides of the main support block are integrally formed with fixed edges, and the fixed edges are fixedly connected to the lower part of the immersion and shaking box by a plurality of first fixing bolts, and both ends of the first support rod are threadedly connected with locking nuts on the outer side of the first support plate.

[0013] Preferably, a spring is fixedly installed on the inner side of the first support plate, the other end of the spring is fixedly connected to a limit plate, the spring and the limit plate are sleeved on the first support rod, and the ends of the limit plates on both sides are fitly connected to the two sides of the main support block.

[0014] Preferably, the auxiliary support block is fixedly connected to the lower part of one end of the immersion and shaking box body by a plurality of second fixing bolts, a first movable connecting block is fixedly provided at the lower part of the auxiliary support block, and the two first crank rods are movably connected to the first movable connecting block by a first movable shaft.

[0015] Preferably, the bonding plate is fixedly connected to the lower part of the other end of the immersion and shaking box through a plurality of third fixing bolts, a second movable connecting block is fixedly provided at the lower part of the bonding plate, and the third crank rod is movably connected to the second movable connecting block through a third movable shaft.

[0016] The application of bioactive peptides produced by proteolysis of royal jelly proteinase, and the application of bioactive peptides produced by hydrolysis of royal jelly proteinase in agents for improving male sperm activity.

[0017] Preferably, when the bioactive peptide is added to the agent for improving male sperm activity, liquid bioactive peptide is directly added, or bioactive peptide powder is mixed and added.

[0018] Compared with the prior art, the present invention has the following beneficial effects:

[0019] When the present invention is in use, the shaking of the immersion and shaking box is achieved through the shaking assembly, and the shaking assembly includes a supporting mechanism and a power mechanism, and the immersion and shaking box is supported by the supporting mechanism, and when the supporting mechanism supports the immersion and shaking box, the immersion and shaking box is kept in active connection, so that the immersion and shaking box can be shaken under the action of the power mechanism, so that the royal jelly protease and royal jelly in the immersion and shaking box can be mixed, and the bioactive peptides hydrolyzed by the royal jelly protease can be layered, and the power mechanism is pushed by the first crank rod and the second crank rod through the servo electric cylinder, so that the immersion and shaking box can be shaken according to the bending degree of the first crank rod and the second crank rod;

[0020] In addition, adding the biologically active polymorphism of royal jelly hydrolyzed into the medicine for improving male sperm activity can effectively improve the body's resistance, increase sperm activity, improve sleep, and make people more energetic. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 It is a schematic diagram of the left structure of the present invention;

[0022] Figure 2 It is a schematic diagram of the right structure of the present invention;

[0023] Figure 3 It is a side view of the shaking assembly of the present invention;

[0024] Figure 4 It is a bottom view of the shaking assembly of the present invention.

[0025] In the figure: 1, base; 2, shaking assembly; 201, supporting mechanism; 2011, first supporting rod; 2012, locking nut; 2013, main supporting block; 2014, fixing edge; 2015, first fixing bolt; 2016, second supporting plate; 2017, first crank rod; 2018, second supporting rod; 2019, first movable shaft; 20110, auxiliary supporting block; 20111, first movable connecting block; 20112, second fixing bolt; 20113, spring; 20114, limit plate; 202, power mechanism; 202 1. The third support plate; 2022. The third support rod; 2023. The second crank rod; 2024. The second movable shaft; 2025. The third crank rod; 2026. The third movable shaft; 2027. The fitting plate; 2028. The second movable connecting block; 2029. The third fixing bolt; 20210. The fourth support plate; 20211. The servo electric cylinder; 20212. The push-pull block; 3. The immersion and shaking box; 4. The first support plate; 5. The discharge pipe; 6. The first valve; 7. The cover plate; 8. The handle; 9. The feed pipe; 10. The second valve; 11. The adjusting groove. DETAILED DESCRIPTION

[0026] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] See also Figure 1-Figure 4 The present invention provides a technical solution: a shaking device for producing bioactive peptides by hydrolyzing royal jelly protease, comprising a base 1, a shaking assembly 2 on the base 1 is connected to a shaking box 3, and the shaking assembly 2 is used to drive the shaking box 3 to shake slowly, so that the bioactive peptides hydrolyzed by royal jelly protease can be dispersed during the shaking process, or the royal jelly protease can continuously hydrolyze the royal jelly;

[0028] The shaking assembly 2 includes a supporting mechanism 201 and a power mechanism 202, first supporting plates 4 are welded on both sides of the middle part of the base 1, a first supporting rod 2011 is movably installed between the first supporting plates 4 on both sides, a main supporting block 2013 is movably installed on the first supporting rod 2011, and the main supporting block 2013 is fixedly installed in the middle of the bottom of the immersion and shaking box 3, second supporting plates 2016 are fixedly installed on both sides of one end of the base 1, and a second supporting rod 2018 is fixedly installed between the second supporting plates 2016 on both sides, and two first crank rods 2017 are movably installed on the second supporting rod 2018, and the ends of the two first crank rods 2017 are connected to one end of the immersion and shaking box 3 through a secondary supporting block 20110;

[0029] The power mechanism 202 includes a third support plate 2021 fixed on both sides of the other end of the base 1, a third support rod 2022 is fixedly installed between the two third support plates 2021, a second crank rod 2023 is movably connected to the third support rod 2022, the end of the second crank rod 2023 is movably connected to the third crank rod 2025 through a second movable shaft 2024, and the end of the third crank rod 2025 is connected to the other end of the immersion and shaking box 3 through a bonding plate 2027;

[0030] Fourth support plates 20210 are welded on both sides of the middle of the base 1, and a servo electric cylinder 20211 is movably installed between the two fourth support plates 20210. The output end of the servo electric cylinder 20211 is fixedly connected to a push-pull block 20212, and the push-pull block 20212 is movably connected to the second movable shaft 2024.

[0031] In order to enable the servo electric cylinder 20211 to be able to adaptively rotate and adjust during pushing adjustment and prevent torsional stress from affecting the power output, in this embodiment, preferably, an adjustment groove 11 is opened at one end of the base 1, and the two fourth support plates 20210 are respectively welded on both sides of the adjustment groove 11, and the servo electric cylinder 20211 is movable inside the adjustment groove 11.

[0032] In order to achieve sealing and shaking of the immersion and shaking box 3, and to facilitate opening, maintenance and cleaning of the interior, and to facilitate the input of raw materials, in the present embodiment, preferably, a cover plate 7 is movably installed on the upper part of the immersion and shaking box 3 through a hinge joint, a handle 8 is fixedly installed on one side of the cover plate 7, one side of the cover plate 7 is connected to a feed pipe 9, and a second valve 10 is fixedly installed on the feed pipe 9.

[0033] In order to discharge the hydrolyzed polypeptides or the stratified polypeptides, in this embodiment, preferably, one end of the immersion and shaking box 3 is fixedly connected to a discharge pipe 5 , and a first valve 6 is fixedly installed on the discharge pipe 5 .

[0034] In order to fix the immersion and shaking box 3 to the main support block 2013 and maintain the balance and stability of the immersion and shaking box 3, in the present embodiment, preferably, both sides of the main support block 2013 are integrally formed with fixed edges 2014, and the fixed edges 2014 are fixedly connected to the lower part of the immersion and shaking box 3 by a plurality of first fixing bolts 2015, and the two ends of the first support rod 2011 are threadedly connected with locking nuts 2012 on the outer side of the first support plate 4.

[0035] In order to achieve stable shaking of the immersion and shaking box 3, springs 20113 and limit plates 20114 are provided on both sides of the main support block 2013 for support to prevent displacement during shaking. In this embodiment, preferably, a spring 20113 is fixedly installed on the inner side of the first support plate 4, and the other end of the spring 20113 is fixedly connected to the limit plate 20114. The spring 20113 and the limit plate 20114 are sleeved on the first support rod 2011, and the ends of the limit plates 20114 on both sides are fitly connected to the two sides of the main support block 2013.

[0036] In order to realize the installation connection between the auxiliary support block 20110 and the immersion and shaking box body 3 and facilitate the support, in the present embodiment, preferably, the auxiliary support block 20110 is fixedly connected to the lower part of one end of the immersion and shaking box body 3 by a plurality of second fixing bolts 20112, and a first movable connecting block 20111 is fixedly provided at the lower part of the auxiliary support block 20110, and the two first crank rods 2017 are movably connected to the first movable connecting block 20111 by a first movable shaft 2019.

[0037] In order to connect the other end of the immersion and shaking box 3 and facilitate the power output of the power mechanism 202 to drive the immersion and shaking box 3 to shake, in this embodiment, preferably, the bonding plate 2027 is fixedly connected to the lower part of the other end of the immersion and shaking box 3 by a plurality of third fixing bolts 2029, and a second movable connecting block 2028 is fixedly provided at the lower part of the bonding plate 2027, and the third crank rod 2025 is movably connected to the second movable connecting block 2028 through a third movable shaft 2026.

[0038] The working principle and use process of the present invention include: using royal jelly protease to produce bioactive peptides, using the bioactive peptides produced after royal jelly protease hydrolysis in a drug for improving male sperm activity, and adding the bioactive peptides to the drug for improving male sperm activity, including adding liquid bioactive peptides directly, or adding bioactive peptides powders in a mixed manner;

[0039] Before the bioactive peptides obtained by hydrolysis of royal jelly protein are added to the agent for improving the activity of male sperm, the royal jelly needs to be hydrolyzed using the equipment described in this application;

[0040] That is, the royal jelly and royal jelly protease are fed into the interior of the immersion and shaking box 3 through the feed pipe 9, and then the power mechanism 202 in the shaking assembly 2 outputs power to drive the immersion and shaking box 3 to shake, and the immersion and shaking box 3 is supported and installed by the support mechanism 201 to maintain the installation stability of the immersion and shaking box 3;

[0041] A first support rod 2011 is installed on both sides of the middle part of the base 1 through the first support plate 4, and a main support block 2013 is movably installed on the first support rod 2011, and the main support block 2013 is fixedly connected to the immersion and shaking box 3 through a fixed edge 2014 and a plurality of first fixing bolts 2015, so as to achieve fixed installation of the immersion and shaking box 3, and in order to maintain the stability of the immersion and shaking box 3, a second support plate 2016 is fixedly installed on both sides of one end of the base 1, and a second support rod 2018 is provided between the second support plates 2016 on both sides, and the second support rod 2018 is connected to a secondary support block 20110 fixed to the lower part of the immersion and shaking box 3 through two first crank rods 2017, so as to support the immersion and shaking box 3 and maintain stability;

[0042] The power mechanism 202 is fixedly provided with a third support rod 2022 between two third support plates 2021 at the other end of the base 1. The third support rod 2022 is movably connected to the third crank rod 2025 through the second crank rod 2023. The end of the third crank rod 2025 is connected to the other end of the immersion and shaking box 3 through the bonding plate 2027. In order to install the servo electric cylinder 20211 and maintain the rotational performance of the servo electric cylinder 20211 during power output, two fourth support plates 20210 are respectively welded on both sides of the adjusting groove 11. The servo electric cylinder 20211 is movable inside the adjusting groove 11. The output end of the servo electric cylinder 20211 is fixedly connected to a push-pull block 20212, and the push-pull block 20212 is movably connected to the second movable shaft 2024.

[0043] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A shaking device for producing bioactive peptides by enzymatic hydrolysis of royal jelly, comprising a base (1), characterized in that: The shaking assembly (2) on the base (1) is connected to a shaking box (3), and the shaking assembly (2) is used to drive the shaking box (3) to shake slowly, so that the bioactive peptides hydrolyzed by the royal jelly protease can be dispersed during the shaking process, or the royal jelly protease can continuously hydrolyze the royal jelly; The shaking assembly (2) comprises a supporting mechanism (201) and a power mechanism (202); first supporting plates (4) are welded on both sides of the middle part of the base (1); a first supporting rod (2011) is movably mounted between the first supporting plates (4) on both sides; a main supporting block (2013) is movably mounted on the first supporting rod (2011); the main supporting block (2013) is fixedly mounted in the middle of the bottom of the immersion and shaking box (3); second supporting plates (2016) are fixedly mounted on both sides of one end of the base (1); a second supporting rod (2018) is fixedly mounted between the second supporting plates (2016) on both sides; two first crank rods (2017) are movably mounted on the second supporting rod (2018); the ends of the two first crank rods (2017) are connected to one end of the immersion and shaking box (3) through auxiliary supporting blocks (20110); The power mechanism (202) comprises a third support plate (2021) fixed on both sides of the other end of the base (1); a third support rod (2022) is fixedly installed between the two third support plates (2021); a second crank rod (2023) is movably connected to the third support rod (2022); an end of the second crank rod (2023) is movably connected to a third crank rod (2025) via a second movable shaft (2024); and an end of the third crank rod (2025) is connected to the other end of the immersion and shaking box (3) via a bonding plate (2027); Fourth support plates (20210) are welded on both sides of the middle of the base (1), a servo electric cylinder (20211) is movably installed between the two fourth support plates (20210), and a push-pull block (20212) is fixedly connected to the output end of the servo electric cylinder (20211), and the push-pull block (20212) is movably connected to the second movable shaft (2024).

2. The immersion and shaking device for producing bioactive peptides by enzymatic hydrolysis of royal jelly according to claim 1, characterized in that: An adjustment groove (11) is provided at one end of the base (1), and the two fourth support plates (20210) are respectively welded on both sides of the adjustment groove (11), and the servo electric cylinder (20211) is movable inside the adjustment groove (11).

3. The immersion and shaking device for producing bioactive peptides by enzymatic hydrolysis of royal jelly according to claim 1, characterized in that: A cover plate (7) is movably mounted on the upper part of the immersion and shaking box (3) through a hinge joint, a handle (8) is fixedly mounted on one side of the cover plate (7), one side of the cover plate (7) is connected to a feed pipe (9), and a second valve (10) is fixedly mounted on the feed pipe (9).

4. The immersion and shaking device for producing bioactive peptides by enzymatic hydrolysis of royal jelly according to claim 1, characterized in that: One end of the immersion and shaking box (3) is fixedly connected to a discharge pipe (5), and a first valve (6) is fixedly installed on the discharge pipe (5).

5. The immersion and shaking device for producing bioactive peptides by enzymatic hydrolysis of royal jelly according to claim 1, characterized in that: The two sides of the main support block (2013) are integrally formed with fixed edges (2014), and the fixed edges (2014) are fixedly connected to the lower part of the immersion and shaking box (3) through a plurality of first fixing bolts (2015), and the two ends of the first support rod (2011) are threadedly connected to locking nuts (2012) on the outer side of the first support plate (4).

6. The immersion and shaking device for producing bioactive peptides by enzymatic hydrolysis of royal jelly according to claim 1, characterized in that: A spring (20113) is fixedly installed on the inner side of the first support plate (4), and the other end of the spring (20113) is fixedly connected to a limiting plate (20114). The spring (20113) and the limiting plate (20114) are sleeved on the first support rod (2011), and the ends of the limiting plates (20114) on both sides are fitted and connected to the two sides of the main support block (2013).

7. The immersion and shaking device for producing bioactive peptides by enzymatic hydrolysis of royal jelly according to claim 1, characterized in that: The auxiliary support block (20110) is fixedly connected to the lower part of one end of the immersion and shaking box (3) through a plurality of second fixing bolts (20112); a first movable connecting block (20111) is fixedly provided at the lower part of the auxiliary support block (20110); and the two first crank rods (2017) are movably connected to the first movable connecting block (20111) through a first movable shaft (2019).

8. The immersion and shaking device for producing bioactive peptides by enzymatic hydrolysis of royal jelly according to claim 1, characterized in that: The bonding plate (2027) is fixedly connected to the lower part of the other end of the immersion and shaking box (3) through a plurality of third fixing bolts (2029); a second movable connecting block (2028) is fixedly provided at the lower part of the bonding plate (2027); and the third crank rod (2025) is movably connected to the second movable connecting block (2028) through a third movable shaft (2026).

9. The use of royal jelly proteinase to produce bioactive peptides, characterized in that: The application of bioactive peptides produced by protease hydrolysis of royal jelly in medicines for improving male sperm activity.

10. The use of royal jelly proteinase to produce bioactive peptides according to claim 9, characterized in that: When the bioactive peptide is added to the agent for improving male sperm activity, the liquid bioactive peptide is directly added, or the bioactive peptide powder is mixed and added.

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