A device for increasing maltitol content

By using lysozyme to eliminate foreign bacteria in the maltose production process and using two layers of ultrafiltration membranes to separate the working area of ​​the enzyme, the problem of heat-resistant microorganisms affecting the saccharification process was solved, and the maltitol content and saccharification efficiency were increased.

CN119875827BActive Publication Date: 2025-09-23SHANDONG JIANYIHONG BIOTECHNOLOGY CO LTD
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Patent Information

Application Number
CN202510369210.6
Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
Filing Date
2025-03-27
Publication Date
2025-09-23
Estimated Expiration
2045-03-27

AI Technical Summary

Technical Problem

In the existing maltose production process, the growth of heat-resistant microorganisms causes the pH value of the saccharification process to decrease, affecting the activity of the enzyme and the saccharification efficiency.

Method used

A device including a saccharification tank, a cylinder, a filtration component and a multi-layer filter membrane is used to eliminate foreign bacteria through lysozyme, and two layers of ultrafiltration membranes are used to separate the working areas of pullulanase and β-amylase to ensure the optimal activity of the enzymes and the saccharification effect.

Benefits of technology

Effectively remove miscellaneous bacteria, increase maltitol content, ensure enzyme activity and saccharification efficiency, and avoid competitive inhibition of enzymes and incomplete saccharification.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention belongs to the technical field of maltose production, and specifically relates to a device for increasing the content of maltitol, which includes a saccharification tank and a cylinder installed on the top of the saccharification tank, a single-layer filter membrane installed in the saccharification tank, a liquid inlet installed on the top of the saccharification tank, a heating component and a liquid inlet component installed on the side wall of the saccharification tank, and a liquid discharge port installed at the bottom of the saccharification tank; the filter assembly includes a push plate and a through-hole base plate, the cylinder output end is connected to the push plate, the push plate is connected to the through-hole base plate by a connecting rod, the single-layer filter membrane is arranged between the push plate and the through-hole base plate, the push plate and the through-hole base plate form a fixed material cavity in the saccharification tank, and the liquid inlet is connected to the fixed material cavity through a threaded hose. When in use, the liquefied liquid is transported to the fixed material cavity through the liquid inlet, and then a certain amount of lysozyme is injected into the fixed material cavity through the liquid inlet component. After the lysozyme hydrolyzes the miscellaneous bacteria in the liquefied liquid, the cylinder pushes the liquefied liquid inside the fixed material cavity through the single-layer filter membrane.
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Description

Technical Field

[0001] The invention belongs to the technical field of maltose production, and particularly relates to a device for increasing the content of maltitol. Background Art

[0002] Maltose, also known as maltobiose, is a disaccharide composed of two glucose molecules linked by an α-1,4 glycosidic bond. It is widely used in a variety of industries, including food, beverages, and pharmaceuticals. Existing maltose production processes primarily include pretreatment, liquefaction, saccharification, and concentration. There are various ways to increase maltitol content, the most common of which is to improve the saccharification process.

[0003] In the existing saccharification process, after liquefaction is complete, the liquefied liquid is filtered to remove excess impurities, yielding a substrate suitable for saccharification. Pullulanase is then added to the substrate for saccharification, followed by β-amylase. Pullulanase cleaves the α-1,6-glycosidic bonds at the branch points of amylose, forming straight chains that make the substrate more accessible to β-amylase. β-amylase primarily acts on amylose, cleaving each α-1,4-linked bond at a time, breaking it down into two glucose groups.

[0004] During the saccharification process, because the pH is near neutral and the temperature has not yet reached the pasteurization temperature, some heat- and acid-resistant microorganisms are prone to growth. Bacteria such as thermophilic lactobacilli consume the produced sugars, multiplying and producing acid. This rapidly lowers the pH to outside the effective range of the enzymes, preventing them from functioning properly. Summary of the Invention

[0005] The purpose of the present invention is to provide a device for increasing the content of maltitol in order to solve the technical problems in the prior art.

[0006] The object of the present invention can be achieved by the following technical scheme: a device for increasing the content of maltitol, which includes a saccharification tank and a cylinder installed on the top of the saccharification tank, the output end of the cylinder is connected to a filter assembly, a single-layer filter membrane is installed in the saccharification tank, a liquid inlet is installed on the top of the saccharification tank, the liquid inlet is connected to the liquefied liquid, a heating assembly and a liquid inlet assembly are respectively installed on the side walls of the saccharification tank, and a liquid discharge port is installed at the bottom of the saccharification tank; the filter assembly includes a push plate and a through-hole base plate, the output end of the cylinder is connected to the push plate, the push plate is connected to the through-hole base plate by a connecting rod, the single-layer filter membrane is arranged between the push plate and the through-hole base plate, the push plate and the through-hole base plate form a dosing chamber in the saccharification tank, and the liquid inlet is connected to the dosing chamber through a threaded hose; when in use, the liquefied liquid is transported to the dosing chamber through the liquid inlet, and then a certain amount of lysozyme is injected into the dosing chamber through the liquid inlet assembly. After the lysozyme hydrolyzes the miscellaneous bacteria in the liquefied liquid, the cylinder pushes the liquefied liquid in the dosing chamber through the single-layer filter membrane.

[0007] As a further optimization or improvement of this solution, the interior of the saccharification tank is divided into a sterilization chamber, a saccharification chamber 1 and a saccharification chamber 2. The sterilization chamber and the saccharification chamber 1 are separated by a single-layer filter membrane, and the saccharification chamber 1 and the saccharification chamber 2 are separated by two layers of ultrafiltration membranes.

[0008] As a further optimization or improvement of this solution, the heating supply component includes heating plate one, heating plate two and heating plate three, and the liquid inlet component includes liquid inlet pipe one, liquid inlet pipe two and liquid inlet pipe three. Heating plate one and liquid inlet pipe one are respectively installed on the outside of the sterilization chamber, heating plate two and liquid inlet pipe two are respectively installed on the outside of the saccharification chamber one, and heating plate three and liquid inlet pipe three are respectively installed on the outside of the saccharification chamber two.

[0009] As a further optimization or improvement of this solution, a through-hole turntable is rotatably installed at the bottom of the through-hole base plate, a fixed box is installed at the bottom of the through-hole turntable, a slide is slidably installed in the fixed box, slide rod one and slide rod two are respectively installed at both ends of the slide, electromagnets are symmetrically installed on the inner wall of the fixed box, the electromagnets adsorb the slide, and a positive J-shaped groove and an inverted J-shaped groove are respectively provided on the two side walls of the saccharification chamber one, slide rod two slides in cooperation with the inverted J-shaped groove, and slide rod one slides in cooperation with the positive J-shaped groove.

[0010] As a further optimization or improvement of this solution, a stirring assembly is installed at the bottom of the through-hole turntable.

[0011] As a further optimization or improvement of this solution, a fixing plate is installed at the bottom of the through-hole turntable, a threaded rod is installed on the fixing plate, an internal threaded tube is rotatably installed in the saccharification chamber, a stirring blade is installed on the internal threaded tube, and the internal threaded tube is transmission-connected to the threaded rod.

[0012] As a further optimization or improvement of this solution, the second liquid inlet tube is connected to pullulanase; and the third liquid inlet tube is connected to β-amylase.

[0013] Beneficial effects of the present invention:

[0014] (1) The present invention eliminates bacteria in the liquefied liquid by adding lysozyme to the liquid, thereby increasing the maltitol content. A dosing chamber is formed by a push plate and a through-hole base plate, and a fixed amount of lysozyme is added according to the specified capacity within the dosing chamber to avoid excessive lysozyme, which may affect beneficial bacteria.

[0015] Specifically, the present invention uses a cylinder to drive the metering chamber downward to filter the hydrolyzed impurities in the liquefied liquid inside the metering chamber through a single-layer filter membrane, thereby preventing the impurities produced by the lysozyme hydrolyzing bacteria from competitively inhibiting the saccharifying enzyme and affecting the enzyme activity and saccharification efficiency.

[0016] (2) The two-layer ultrafiltration membrane of the present invention is a double-layer ultrafiltration membrane, one of which is used to block the macromolecular starch in the bottom liquid. When pullulanase cuts the starch into linear chains, it can pass through the two layers of ultrafiltration membrane; the other layer of ultrafiltration membrane is used to separate pullulanase and β-amylase, ensuring that pullulanase is located in saccharification cavity one and β-amylase is located in saccharification cavity two. At the same time, saccharification cavity one and saccharification cavity two are coordinated to maximize the activity of pullulanase and β-amylase.

[0017] The present invention uses two layers of ultrafiltration membranes to block macromolecular starch in the bottom liquid inside the saccharification chamber one, allowing the pullulanase in the optimal activity state to cut the starch into linear chains before entering the saccharification chamber two, ensuring that the β-amylase in the saccharification chamber two mainly decomposes the linear starch decomposed by the pullulanase again, avoiding the β-amylase acting on the starch not decomposed by the pullulanase, resulting in premature consumption of the β-amylase and ultimately incomplete saccharification. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention will be further described below with reference to the accompanying drawings.

[0019] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0020] Figure 2 It is a cross-sectional view of the overall structure of the present invention.

[0021] Figure 3 It is a schematic diagram of the internal overall structure of the present invention.

[0022] Figure 4 This is a schematic diagram of the installation location of the filter component.

[0023] Figure 5 Schematic diagram of the overall structure of the filter component.

[0024] Figure 6 Schematic diagram of the bottom structure of the through-hole turntable.

[0025] Figure 7 Schematic diagram of the internal structure of the fixed box.

[0026] Figure 8 This is the matching diagram of the positive J-shaped groove and the slide bar 1.

[0027] Figure 9 This is the matching diagram of the inverted J-shaped groove and the second slide bar.

[0028] The following are marked in the figure: 1. Mashing tank; 101. Sterilization chamber; 102. Mashing chamber 1; 103. Mashing chamber 2; 2. Cylinder; 3. Liquid inlet; 4. Heating assembly; 401. Heating plate 1; 402. Heating plate 2; 403. Heating plate 3; 5. Threaded hose; 6. Filter assembly; 601. Push plate; 602. Through-hole base plate; 603. Sealing gasket; 604. Through-hole turntable; 605. Sliding rod 1; 606. Connecting rod ; 607, slide bar 2; 608, fixed box; 609, slide plate; 610, electromagnet; 7, stirring assembly; 701, fixed plate; 702, threaded rod; 703, stirring blade; 704, internal threaded tube; 8, liquid inlet assembly; 801, liquid inlet pipe 1; 802, liquid inlet pipe 2; 803, liquid inlet pipe 3; 9, discharge port; 11, single-layer filter membrane; 12, two-layer ultrafiltration membrane; 13, positive J-shaped groove; 14, inverted J-shaped groove. DETAILED DESCRIPTION

[0029] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making any creative efforts shall fall within the scope of protection of the present invention.

[0030] See also Figures 1-6 A device for increasing the content of maltitol comprises a saccharification tank 1 and a cylinder 2 mounted on the top of the saccharification tank 1, the output end of the cylinder 2 is connected to a filter assembly 6, a single-layer filter membrane 11 is installed in the saccharification tank 1, a liquid inlet 3 is installed on the top of the saccharification tank 1, the liquid inlet 3 is connected to the liquefied liquid, a heating assembly 4 and a liquid inlet assembly 8 are respectively mounted on the side walls of the saccharification tank 1, and a drain port 9 is installed at the bottom of the saccharification tank 1; the filter assembly 6 comprises a push plate 601 and a through-hole base plate 602, the output end of the cylinder 2 is connected to the push plate 601, the push plate 601 It is connected to the through-hole base plate 602 through a connecting rod 606, and the single-layer filter membrane 11 is arranged between the push plate 601 and the through-hole base plate 602. The push plate 601 and the through-hole base plate 602 form a dosing chamber in the saccharification tank 1, and the liquid inlet 3 is connected to the dosing chamber through a threaded hose 5; when in use, the liquefied liquid is transported to the dosing chamber through the liquid inlet 3, and then a certain amount of lysozyme is injected into the dosing chamber through the liquid inlet component 8. After the lysozyme hydrolyzes the miscellaneous bacteria in the liquefied liquid, the cylinder 2 pushes the liquefied liquid in the dosing chamber through the single-layer filter membrane 11.

[0031] Specifically, a through-hole turntable 604 is rotatably installed at the bottom of the through-hole base plate 602, a fixed box 608 is installed at the bottom of the through-hole turntable 604, a slide plate 609 is slidably installed in the fixed box 608, a slide bar 1 605 and a slide bar 2 607 are respectively installed at both ends of the slide plate 609, an electromagnet 610 is symmetrically installed on the inner wall of the fixed box 608, and the electromagnet 610 adsorbs the slide plate 609, and the two side walls of the saccharification chamber 102 are respectively provided with a positive J-shaped groove 13 and an inverted J-shaped groove 14 that are symmetrical in upper and lower directions, the slide bar 2 607 slides in cooperation with the inverted J-shaped groove 14, and the slide bar 1 605 slides in cooperation with the positive J-shaped groove 13.

[0032] It should be noted that, see Figure 2 In the initial state, the metering chamber composed of the pushing plate 601 and the through-hole base plate 602 is located in the sterilization chamber 101. At this time, the through-hole base plate 602 is located on one side of the single-layer filter membrane 11, and the pushing plate 601 is located on the other side of the single-layer filter membrane 11. The through-hole turntable 604 blocks the through-hole base plate 602, so that the metering chamber is in a sealed state.

[0033] During use, liquefied liquid is added to the dosing chamber through liquid inlet 3. When the dosing chamber reaches a specified volume, liquid inlet 3 is closed, and liquid inlet pipe 1 (801) is opened. Lysozyme is then injected into the dosing chamber through liquid inlet pipe 1 (801) at a predetermined ratio. After the lysozyme hydrolyzes the bacteria in the liquefied liquid, cylinder 2 is activated, driving the dosing chamber downward. At this point, push plate 601 pushes the hydrolyzed impurities in the liquefied liquid inside the dosing chamber through single-layer filter membrane 11. During this process, the lysozyme's activity is optimized by adjusting heating plate 1 (401).

[0034] The present invention eliminates foreign bacteria in the liquefied liquid by adding lysozyme to the liquid, thereby increasing the maltitol content. To prevent excessive lysozyme from affecting beneficial bacteria, the present invention comprises a dosing chamber formed by a push plate 601 and a through-hole base plate 602, into which a fixed amount of lysozyme is added according to a specified volume within the dosing chamber. Lysozyme hydrolyzes impurities produced by bacteria, which competitively inhibits saccharifying enzymes, affecting enzyme activity and saccharification efficiency. Therefore, the present invention uses a cylinder 2 to drive the dosing chamber downward, filtering the hydrolyzed impurities in the liquefied liquid within the dosing chamber through a single-layer filter membrane 11.

[0035] It should be noted that in the initial state, slide bar 1 605 is inserted into the regular J-shaped groove 13. At this point, slide bar 1 605 slidably engages with the regular J-shaped groove 13, while slide bar 2 607 is separated from the inverted J-shaped groove 14. Regular J-shaped groove 13 is J-shaped. When slide bar 1 605 initially slides along the vertical section of regular J-shaped groove 13, the through-hole base plate 602 and the through-hole rotating disk 604 remain in the same state, with the through-hole rotating disk 604 blocking the through-hole base plate 602. When slide bar 1 605 slides to the end of regular J-shaped groove 13, slide bar 1 605 and the through-hole rotating disk 604 rotate, causing the through-hole rotating disk 604 to overlap with the through-holes on the through-hole base plate 602. At this point, the through-hole base plate 602 is in a liquid-permeable state, and all the bottom liquid within the dosing chamber enters the saccharification chamber 102. The inverted J-shaped groove 14 is also in an inverted J-shaped design, and its operating principle is the same as that of slide bar 1 605 and regular J-shaped groove 13.

[0036] As the cylinder 2 starts, the cylinder 2 drives the metering chamber to move from the sterilization chamber 101 toward the saccharification chamber 102, and the push plate 601 pushes the liquefied liquid inside the metering chamber to be filtered through the single-layer filter membrane 11. The filtered bottom liquid passes through the single-layer filter membrane 11 and enters the saccharification chamber 102. At the same time, the slide bar 1605 at the bottom of the through-hole turntable 604 slides in the regular J-shaped groove 13. When the liquefied liquid inside the metering chamber has completely passed through the single-layer filter membrane 11, the slide bar 1605 slides to the tail end of the regular J-shaped groove 13, and the slide bar 1605 and the through-hole turntable 604 rotate so that the through-hole turntable 604 coincides with the through-hole on the through-hole base plate 602. At this time, the through-hole base plate 602 is in a liquid-passing state, and the bottom liquid inside the metering chamber all enters the saccharification chamber 102.

[0037] When the dosing chamber needs to return, the cylinder 2 drives the push plate 601 and the through-hole base plate 602 to move upward, and at the same time activates the electromagnet 610. The electromagnet 610 attracts the slide plate 609, causing the slide bar 1 605 to disengage from the positive J-shaped groove 13, while the slide bar 2 607 is inserted into the inverted J-shaped groove 14. As the cylinder 2 drives the dosing chamber upward, the through-hole base plate 602 is in a liquid-passing state, preventing the through-hole base plate 602 from drawing the bottom liquid inside the saccharification chamber 2 103 toward the saccharification chamber 1 102, causing damage to the two layers of ultrafiltration membrane 12. After the slide bar 2 607 reaches the top of the inverted J-shaped groove 14, the through-hole turntable 604 rotates and blocks the through-hole base plate 602. It should be noted that a sealing gasket 603 is installed at the bottom of the through-hole base plate 602 to improve the sealing performance of the through-hole base plate 602 and the sealing gasket 603.

[0038] See also Figure 1-Figure 5 The interior of the saccharification tank 1 is divided into a sterilization chamber 101, a saccharification chamber 1 102 and a saccharification chamber 2 103. The sterilization chamber 101 and the saccharification chamber 1 102 are separated by a single-layer filter membrane 11, and the saccharification chamber 1 102 and the saccharification chamber 2 103 are separated by two layers of ultrafiltration membranes 12.

[0039] Specifically, the heating component 4 includes a heating plate 1 401, a heating plate 2 402 and a heating plate 3 403, and the liquid inlet component 8 includes a liquid inlet pipe 1 801, a liquid inlet pipe 2 802 and a liquid inlet pipe 3 803. The heating plate 1 401 and the liquid inlet pipe 1 801 are respectively installed on the outside of the sterilization chamber 101, the heating plate 2 402 and the liquid inlet pipe 2 802 are respectively installed on the outside of the saccharification chamber 102, and the heating plate 3 403 and the liquid inlet pipe 3 803 are respectively installed on the outside of the saccharification chamber 2 103.

[0040] Specifically, the second liquid inlet pipe 802 is connected to pullulanase; the third liquid inlet pipe 803 is connected to β-amylase.

[0041] It should be noted that the saccharification method of the prior art is to first add pullulanase to the base liquid and finally add β-amylase. However, this method has the following technical problems: first, the optimal activity temperatures of pullulanase and β-amylase are different. When pullulanase and β-amylase work together, the activity of one of them will inevitably be suppressed, thereby affecting the saccharification efficiency; second, when the pullulanase has not yet cut all the starch in the base liquid into straight chains, β-amylase is added. The β-amylase will decompose the uncut starch, leaving the pullulanase without a target, resulting in excessive pullulanase residue; as the target of β-amylase increases, the β-amylase is consumed prematurely, ultimately resulting in incomplete saccharification.

[0042] It should be noted that the two layers of ultrafiltration membrane 12 are double-layer ultrafiltration membranes, one of which is used to block the large molecular starch in the bottom liquid. When pullulanase cuts the starch into linear chains, it can pass through the two layers of ultrafiltration membrane 12; the other layer of ultrafiltration membrane is used to block and separate pullulanase and β-amylase, ensuring that the pullulanase is located in the saccharification cavity 1 102 and the β-amylase is located in the saccharification cavity 2 103. At the same time, the saccharification cavity 102 and the saccharification cavity 2 103 are coordinated to maximize the activity of pullulanase and β-amylase.

[0043] The present invention uses two layers of ultrafiltration membranes 12 to block the macromolecular starch in the bottom liquid inside the saccharification chamber 102, allowing the pullulanase in the optimal activity state to cut the starch into linear chains before entering the saccharification chamber 2 103, ensuring that the β-amylase in the saccharification chamber 2 103 mainly decomposes the linear starch decomposed by the pullulanase again, avoiding the β-amylase from acting on the starch not decomposed by the pullulanase, resulting in premature consumption of the β-amylase, and ultimately causing incomplete saccharification.

[0044] Pullulanase is added to the saccharification chamber 102 through the liquid inlet pipe 2 802, and the starch in the bottom liquid is cut into straight chains by the pullulanase. The decomposed straight chain starch enters the saccharification chamber 2 103 through two layers of ultrafiltration membrane 12, and then β-amylase is added to the inside of the saccharification chamber 2 103 through the liquid inlet pipe 3 803, so that the β-amylase mainly acts on the straight chain starch cut by the pullulanase to ensure thorough saccharification.

[0045] Specifically, when the cylinder 2 drives the push plate 601 and the through-hole base plate 602 to move downward, since the through-hole turntable 604 blocks the through-hole base plate 602, the through-hole base plate 602 and the through-hole turntable 604 will compress the air inside the saccharification chamber 102, accelerate the amylose inside the saccharification chamber 102 to pass through the two layers of ultrafiltration membrane 12, and increase the saccharification rate.

[0046] See also Figure 2-Figure 6 A stirring assembly 7 is installed at the bottom of the through-hole turntable 604.

[0047] A fixing plate 701 is installed at the bottom of the through-hole turntable 604, and a threaded rod 702 is installed on the fixing plate 701. An internal threaded tube 704 is rotatably installed in the saccharification chamber 102, and a stirring blade 703 is installed on the internal threaded tube 704. The internal threaded tube 704 is transmission-connected to the threaded rod 702.

[0048] It should be noted that when the cylinder 2 is running, the threaded rod 702 at the bottom of the through-hole turntable 604 moves synchronously with the through-hole turntable 604. Through the threaded connection between the threaded rod 702 and the internal threaded tube 704, the movement of the threaded rod 702 drives the internal threaded tube 704 and the stirring blade 703 to rotate, thereby stirring the bottom liquid inside the saccharification chamber 102 and accelerating the saccharification efficiency.

[0049] The implementation principle of the present invention is: in the initial state, the metering chamber composed of the push plate 601 and the through-hole base plate 602 is located in the sterilization chamber 101. At this time, the through-hole base plate 602 is located on one side of the single-layer filter membrane 11, and the push plate 601 is located on the other side of the single-layer filter membrane 11. The through-hole turntable 604 blocks the through-hole base plate 602, so that the metering chamber is in a sealed state.

[0050] During use, liquefied liquid is added to the dosing chamber through liquid inlet 3. When the dosing chamber reaches a specified volume, liquid inlet 3 is closed, and liquid inlet pipe 1 (801) is opened. Lysozyme is then injected into the dosing chamber through liquid inlet pipe 1 (801) at a predetermined ratio. After the lysozyme hydrolyzes the bacteria in the liquefied liquid, cylinder 2 is activated, driving the dosing chamber downward. At this point, push plate 601 pushes the hydrolyzed impurities in the liquefied liquid inside the dosing chamber through single-layer filter membrane 11. During this process, the lysozyme's activity is optimized by adjusting heating plate 1 (401).

[0051] As the cylinder 2 is started, the cylinder 2 drives the metering chamber to move from the sterilization chamber 101 toward the saccharification chamber 102, and the push plate 601 pushes the liquefied liquid inside the metering chamber to be filtered through the single-layer filter membrane 11. The filtered bottom liquid passes through the single-layer filter membrane 11 and enters the saccharification chamber 102. At the same time, the slide bar 1 605 at the bottom of the through-hole turntable 604 slides in the regular J-shaped groove 13. When the liquefied liquid inside the metering chamber has completely passed through the single-layer filter membrane 11, the slide bar 1 605 slides to the tail end of the regular J-shaped groove 13. Under the cooperation of the slide bar 1 605 and the regular J-shaped groove 13, the slide bar 1 605 and the through-hole turntable 604 rotate, so that the through-hole turntable 604 coincides with the through-hole on the through-hole base plate 602. At this time, the through-hole base plate 602 is in a liquid-passing state, and the bottom liquid inside the metering chamber all enters the saccharification chamber 102.

[0052] Pullulanase is added to the saccharification chamber 102 through the liquid inlet pipe 2 802, and the starch in the bottom liquid is cut into straight chains by the pullulanase. The decomposed straight chain starch enters the saccharification chamber 2 103 through two layers of ultrafiltration membrane 12, and then β-amylase is added to the inside of the saccharification chamber 2 103 through the liquid inlet pipe 3 803, so that the β-amylase mainly acts on the straight chain starch cut by the pullulanase to ensure thorough saccharification.

[0053] When the dosing chamber needs to return, cylinder 2 moves the push plate 601 and the through-hole base plate 602 upward, activating electromagnet 610. Electromagnet 610 attracts slide plate 609, disengaging slide bar 1 605 from the positive J-shaped groove 13 while slide bar 2 607 inserts into the inverted J-shaped groove 14. As cylinder 2 drives the dosing chamber upward, the through-hole base plate 602 remains fluid-free, preventing it from drawing the bottom liquid from saccharification chamber 2 103 toward saccharification chamber 1 102, potentially damaging the two ultrafiltration membranes 12. Once slide bar 2 607 reaches the top of the inverted J-shaped groove 14, the through-hole turntable 604 rotates, sealing the through-hole base plate 602.

[0054] Specifically, the two layers of ultrafiltration membrane 12 are double-layer ultrafiltration membranes, one of which is used to block the large molecular starch in the bottom liquid. When pullulanase cuts the starch into linear chains, it can pass through the two layers of ultrafiltration membrane 12; the other layer of ultrafiltration membrane is used to separate pullulanase and β-amylase, ensuring that the pullulanase is located in the saccharification chamber 1 102 and the β-amylase is located in the saccharification chamber 2 103. At the same time, the saccharification chamber 102 and the saccharification chamber 2 103 are coordinated to maximize the activity of pullulanase and β-amylase.

[0055] The present invention uses two layers of ultrafiltration membranes 12 to block the macromolecular starch in the bottom liquid inside the saccharification chamber 102, allowing the pullulanase in the optimal activity state to cut the starch into linear chains before entering the saccharification chamber 2 103, ensuring that the β-amylase in the saccharification chamber 2 103 mainly decomposes the linear starch decomposed by the pullulanase again, avoiding the β-amylase from acting on the starch not decomposed by the pullulanase, resulting in premature consumption of the β-amylase, and ultimately causing incomplete saccharification.

[0056] Specifically, when the cylinder 2 is running, the threaded rod 702 at the bottom of the through-hole turntable 604 moves synchronously with the through-hole turntable 604. Through the threaded connection between the threaded rod 702 and the internal threaded tube 704, the movement of the threaded rod 702 drives the internal threaded tube 704 and the stirring blade 703 to rotate, thereby stirring the bottom liquid inside the saccharification chamber 102 and accelerating the saccharification efficiency.

[0057] Specifically, when the cylinder 2 drives the push plate 601 and the through-hole base plate 602 to move downward, since the through-hole turntable 604 blocks the through-hole base plate 602, the through-hole base plate 602 and the through-hole turntable 604 will compress the air inside the saccharification chamber 102, accelerate the amylose inside the saccharification chamber 102 to pass through the two layers of ultrafiltration membrane 12, and increase the saccharification rate.

[0058] The basic principles, main features, and advantages of the present invention are shown and described above. Those skilled in the art should understand that the present invention is not limited to the above embodiments. The above embodiments and descriptions are merely illustrative of the principles of the present invention. Various changes and modifications may be made to the present invention without departing from the spirit and scope of the present invention, and such changes and modifications fall within the scope of the invention as claimed.

Claims

1. A device for increasing the content of maltitol, characterized in that: The invention comprises a saccharification tank (1) and a cylinder (2) installed on the top of the saccharification tank (1), wherein the output end of the cylinder (2) is connected to a filter assembly (6), a single-layer filter membrane (11) is installed in the saccharification tank (1), a liquid inlet (3) is installed on the top of the saccharification tank (1), and the liquid inlet (3) is connected to a hydraulic fluid, a heating assembly (4) and a liquid inlet assembly (8) are respectively installed on the side walls of the saccharification tank (1), and a liquid discharge port (9) is installed at the bottom of the saccharification tank (1); The filter assembly (6) includes a push plate (601) and a through-hole base plate (602), the output end of the cylinder (2) is connected to the push plate (601), the push plate (601) is connected to the through-hole base plate (602) via a connecting rod (606), the single-layer filter membrane (11) is arranged between the push plate (601) and the through-hole base plate (602), the push plate (601) and the through-hole base plate (602) form a fixed material cavity in the saccharification tank (1), and the liquid inlet (3) is connected to the fixed material cavity via a threaded hose (5); During use, the liquefied liquid is transported to the dosing chamber through the liquid inlet (3), and then a fixed amount of lysozyme is injected into the dosing chamber through the liquid inlet assembly (8). After the lysozyme hydrolyzes the bacteria in the liquefied liquid, the cylinder (2) pushes the liquefied liquid in the dosing chamber through the single-layer filter membrane (11).

2. The device for increasing the maltitol content according to claim 1, wherein: The interior of the saccharification tank (1) is divided into a sterilization chamber (101), a saccharification chamber 1 (102) and a saccharification chamber 2 (103). The sterilization chamber (101) and the saccharification chamber 1 (102) are separated by a single-layer filter membrane (11), and the saccharification chamber 1 (102) and the saccharification chamber 2 (103) are separated by two layers of ultrafiltration membranes (12).

3. The device for increasing the maltitol content according to claim 2, wherein: The heating component (4) includes a heating plate 1 (401), a heating plate 2 (402) and a heating plate 3 (403); the liquid inlet component (8) includes a liquid inlet pipe 1 (801), a liquid inlet pipe 2 (802) and a liquid inlet pipe 3 (803); the outside of the sterilization chamber (101) is respectively installed with the heating plate 1 (401) and the liquid inlet pipe 1 (801); the outside of the saccharification chamber 1 (102) is respectively installed with the heating plate 2 (402) and the liquid inlet pipe 2 (802); the outside of the saccharification chamber 2 (103) is respectively installed with the heating plate 3 (403) and the liquid inlet pipe 3 (803).

4. The device for increasing the maltitol content according to claim 2, wherein: A through-hole turntable (604) is rotatably mounted on the bottom of the through-hole base plate (602), a fixed box (608) is mounted on the bottom of the through-hole turntable (604), a slide plate (609) is slidably mounted in the fixed box (608), a slide bar 1 (605) and a slide bar 2 (607) are mounted on both ends of the slide plate (609), an electromagnet (610) is symmetrically mounted on the inner wall of the fixed box (608), and the electromagnet (610) adsorbs the slide plate (609), and a positive J-shaped groove (13) and an inverted J-shaped groove (14) are respectively provided on the two side walls of the saccharification chamber 1 (102), the slide bar 2 (607) is slidably matched with the inverted J-shaped groove (14), and the slide bar 1 (605) is slidably matched with the positive J-shaped groove (13).

5. The device for increasing the maltitol content according to claim 4, wherein: A stirring assembly (7) is installed at the bottom of the through-hole turntable (604).

6. The device for increasing the maltitol content according to claim 5, wherein: A fixing plate (701) is installed at the bottom of the through-hole turntable (604), the fixing plate (701) is set in a U shape, the fixing box (608) is located inside the fixing plate (701), and a threaded rod (702) is installed on the fixing plate (701). An internal threaded tube (704) is rotatably installed in the saccharification chamber (102), a stirring blade (703) is installed on the internal threaded tube (704), and the internal threaded tube (704) is transmission-connected to the threaded rod (702).

7. The device for increasing the maltitol content according to claim 3, wherein: The second liquid inlet tube (802) is connected to pullulanase; the third liquid inlet tube (803) is connected to β-amylase.

Citation Information

Patent Citations

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