Preparation method of composite strain for decomposing municipal sludge

Abstract

The invention discloses a preparation method of a composite strain for decomposing municipal sludge, which comprises the following steps: screening and preparing composite strain balls: S1, selecting degradable plant raw materials to prepare two symmetrical spherical shells; s2, preparing composite strain nutrition balls; s3, degradable plant raw materials are selected to prepare a composite strain culture bin; s4, the composite strain nutrition balls are combined with the two symmetrical spherical shells; s5, drilling a hole from the outside of the curved spherical shell to extend into the nutrition ball; s6, the columnar protruding parts of the composite strain culture bins are inserted into the nutrition balls along the holes; s7, after assembly is completed, standing is conducted for 1-3 days, and the breeding condition of the strains is observed through infrared induction equipment; s8, when the temperature is continuously reduced in the strain breeding process, the composite strains can be subjected to freezing short-period storage; s9, the survival condition of the composite strains is sampled and detected, the composite fungus balls are smashed, bagged and frozen for long-period storage, the composite strains can be freely combined according to actual use requirements, and the problem of municipal sludge decomposition is solved.

Description

Technical Field

[0001] The invention relates to the technical field of urban sludge treatment, in particular to a method for preparing composite bacteria for decomposing urban sludge. Background Art

[0002] With the development of urban construction, domestic sewage has increased significantly. Since domestic sewage is rich in harmful substances, it cannot be discharged directly into rivers. Urban sewage is discharged into rivers after treatment, and the precipitated sludge can be used as organic fertilizer after treatment. However, there are still many pollutants in urban sewage sludge, which needs to be treated to meet agricultural use standards. Urban sludge can decompose and treat most of the harmful substances inside by adding composite bacteria. Traditional composite bacteria are usually dry powder, which is directly mixed into the sludge. It takes a certain amount of time for the composite bacteria to reproduce, and multiple bacteria mixed together may interfere with each other and affect their growth. Composite bacteria lack the stage of fusion and symbiosis, and the storage period of composite bacteria is relatively short. Therefore, how to solve the above-mentioned problems has become a difficult problem that needs to be solved urgently. Summary of the invention

[0003] The object of the present invention is to provide a method for preparing composite bacteria for decomposing urban sludge, which can freely combine composite bacteria according to actual use needs and solve the problem of decomposing urban sludge.

[0004] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a method for preparing a composite bacterial strain for decomposing urban sludge, which includes screening and preparing composite bacterial strain balls, S1, selecting degradable plant raw materials to prepare two symmetrical spherical shells; S2, preparing composite bacterial strain nutrient balls; S3, selecting degradable plant raw materials to prepare composite bacterial strain culture chambers; S4, combining the composite bacterial strain nutrient balls with the two symmetrical spherical shells; S5, drilling holes from the outside of the curved spherical shells to the inside of the nutrient balls; S6, inserting the columnar protrusions of the composite bacterial strain culture chamber into the inside of the nutrient balls along the holes; S7, after assembly, standing for 1-3 days, and using infrared sensing equipment to observe the reproduction of the bacteria; S8, when the temperature continues to drop during the reproduction of the bacteria, the composite bacteria can be frozen for short-term storage; S9, sampling to detect the survival of the composite bacteria, and crushing, bagging and freezing the composite bacterial balls for long-term storage.

[0005] Preferably, step S1: collect plant straw, leaves, shrub branches, etc., use a crusher and a grinder to process the collected materials into powder with a size of 0.1-0.5 mm in diameter, add water and mix until it becomes viscous, use professional equipment to extrude the above raw materials to prepare two spherical shells, the size of the spherical shells is controlled to be 5-10 cm in diameter, and after the spherical shells are formed, they are placed in a ventilated and shaded place to dry, and one of the spherical shells has a reserved nutrient diversion port.

[0006] Preferably, step S2: use plant materials to wrap the nutrients required for the growth of the composite bacteria, the nutrients can be solid or liquid, a nutrient filling port with a tubular structure extends from the top of the composite bacteria nutrient ball, and the size of the composite bacteria nutrient ball is smaller than the size of the ball shell.

[0007] Preferably, step S3: the composite bacterial strain culture chamber is divided into two parts, one part is the bacterial strain storage space, which is a spherical structure, and the other part is a columnar protruding tube, which is connected to the bacterial strain storage space. Nutrients are placed inside the columnar protruding tube to prevent the bacterial strains from gathering inside the composite bacterial strain nutrient ball for a short time. The bacterial strains are added after the overall assembly is completed.

[0008] Preferably, step S4: first glue the reserved nutrient diversion port of the spherical shell and the nutrient filling port together, and then glue the two spherical shells together to form a closed space. The adhesive can be yellow mud, plant glue, etc. The nutrients are added after the overall assembly is completed. Yellow mud, plant glue, etc. are used to fix the foundation between the composite bacteria nutrient ball and the spherical shell. After the foundation is fixed, let it stand for 3-5 hours.

[0009] Preferably, step S5: drilling a hole from the outside of the preliminarily assembled spherical shell according to the size of the columnar protruding tube, and the drilling hole extends to the inside of the composite bacteria nutrient ball.

[0010] Preferably, step S6: different bacterial strains are selected according to the actual situation of urban sludge treatment, and the selected bacterial strains are respectively injected into the composite bacterial strain culture bin, and then the composite bacterial strain culture bin is assembled together with the spherical shell and the composite bacterial strain nutrient ball through a columnar protruding tube, and the connecting gaps are sealed with yellow mud, plant glue, etc., and non-anaerobic bacterial strains are selected without sealing.

[0011] Preferably, step S7: after the assembly is completed, the ball is left to stand for a period of time to observe whether there is leakage, the assembled composite bacterial species ball is placed in a constant temperature space, and the reproduction of the bacterial species and the fusion and symbiosis of multiple bacterial species are observed by infrared sensing equipment.

[0012] Preferably, step S8: monitor the temperature distribution of the composite bacteria ball at multiple points to comprehensively judge whether the reproduction of the composite bacteria has reached its peak. When the activity of the composite bacteria decreases and the monitored temperature begins to decrease, the composite bacteria ball is transferred to a freezing warehouse for storage. The freezing temperature is maintained at 0°C. During the storage period, the survival of the composite bacteria is sampled and tested to ensure that the storage time can reach 3-15 days.

[0013] Preferably, step S9: sample and test the short-term stored composite bacteria once every three days. If the survival rate of the composite bacteria is reduced, the composite bacteria balls that cannot be used in a short time are crushed and bagged and placed in a freezing warehouse at -15°C to -30°C to ensure that the storage time can be extended to 30-90 days.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] (1) The present invention can freely combine the number and types of composite bacterial strains according to actual use needs to solve the problem of urban sludge treatment. The present invention constructs a symbiotic environment of multiple bacterial strains by placing the bacterial strains separately, so that the bacterial strains can adapt to each other and avoid affecting each other's reproduction and growth in the later stage.

[0016] (2) The composite bacterial strain prepared by the present invention can be directly thrown into sewage for use, or can be crushed and mixed into sludge for use, thereby constructing a breeding and growth environment in advance. When mixed into sewage, it can gradually dissolve and achieve rapid degradation of impurities in sewage. The storage time is equivalent to that of traditional dry powder composite bacterial strains. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall preparation process of the present invention. DETAILED DESCRIPTION

[0018] In the following description, a large number of specific details are given in order to provide a more thorough understanding of the present invention. However, it is obvious to those skilled in the art that the present invention can be implemented without one or more of these details. In other examples, in order to avoid confusion with the present invention, some technical features known in the art are not described. In order to enable those skilled in the art to better understand the present application scheme, the technical scheme in the embodiment of the present application will be clearly and completely described below in conjunction with the drawings in the embodiment of the present application. Obviously, the described embodiment is only an embodiment of a part of the present application, not all embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians of the art without making creative work should belong to the scope of protection of this application.

[0019] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other. The present application will be described in detail below with reference to the accompanying drawings and in conjunction with the implementation regulations.

[0020] like Figure 1As shown, a preparation method of composite bacteria for decomposing urban sludge includes screening and preparing composite bacteria balls, S1, selecting degradable plant materials to prepare two symmetrical spherical shells; S2, preparing composite bacteria nutrient balls; S3, selecting degradable plant materials to prepare composite bacteria culture chambers; S4, combining the composite bacteria nutrient balls with the two symmetrical spherical shells; S5, drilling holes from the outside of the curved spherical shells to the inside of the nutrient balls; S6, inserting the columnar protrusions of the composite bacteria culture chamber into the inside of the nutrient balls along the holes; S7, after the assembly is completed, standing for 1-3 days, and using infrared sensing equipment to observe the reproduction of the bacteria; S8, when the temperature continues to drop during the reproduction of the bacteria, the composite bacteria can be frozen for short-term storage; S9, sampling and testing the survival of the composite bacteria, crushing the composite bacteria balls, bagging and freezing them for long-term storage, and using a small amount, they can be directly soaked in water to dissolve, and then a small amount can be extracted and mixed into sewage for use, and can also be directly crushed and scattered for use.

[0021] Step S1: Collect plant straw, leaves, shrub branches, etc., use a crusher and a grinder to process the collected materials into powder with a diameter of 0.1-0.5 mm, add water and mix until it becomes viscous, use professional equipment to extrude the above raw materials to prepare two spherical shells, the diameter of the spherical shells is controlled at 5-10 cm, and after the spherical shells are formed, place them in a ventilated and shaded place to dry, and one of the spherical shells has a reserved nutrient diversion port.

[0022] Step S2: using plant materials to wrap nutrients required for the growth of the composite bacteria, the nutrients can be solid or liquid, a nutrient filling port with a tubular structure extends from the top of the composite bacteria nutrient ball, and the size of the composite bacteria nutrient ball is smaller than the size of the ball shell.

[0023] Preferably, step S3: the composite bacterial strain culture chamber is divided into two parts, one part is the bacterial strain storage space, which is a spherical structure, and the other part is a columnar protruding tube, which is connected to the bacterial strain storage space. Nutrients are placed inside the columnar protruding tube to prevent the bacterial strains from gathering inside the composite bacterial strain nutrient ball for a short time. The bacterial strains are added after the overall assembly is completed.

[0024] Preferably, step S4: first glue the reserved nutrient diversion port of the spherical shell and the nutrient filling port together, and then glue the two spherical shells together to form a closed space. The adhesive can be yellow mud, plant glue, etc. The nutrients are added after the overall assembly is completed. Yellow mud, plant glue, etc. are used to fix the foundation between the composite bacteria nutrient ball and the spherical shell. After the foundation is fixed, let it stand for 3-5 hours.

[0025] Step S5: drilling holes from the outside of the preliminarily assembled spherical shell according to the size of the columnar protruding tube, and the holes are extended to the inside of the composite bacteria nutrient ball.

[0026] Step S6: different bacterial strains are selected according to the actual situation of urban sludge treatment, and the selected bacterial strains are respectively injected into the composite bacterial strain culture bin, and then the composite bacterial strain culture bin is assembled together with the spherical shell and the composite bacterial strain nutrient ball through a columnar protruding tube, and the connecting gaps are sealed with yellow mud, plant glue, etc., and non-anaerobic bacterial strains are selected without sealing.

[0027] Step S7: After the assembly is completed, the mixture is allowed to stand for a period of time to observe whether there is leakage. The assembled composite bacterial species ball is placed in a constant temperature space, and the reproduction of the bacterial species and the fusion and symbiosis of multiple bacterial species are observed through infrared sensing equipment.

[0028] Step S8: By monitoring the temperature distribution of the composite bacteria balls at multiple points, comprehensively judge whether the reproduction of the composite bacteria has reached its peak. When the activity of the composite bacteria decreases and the monitored temperature begins to decrease, the composite bacteria balls are transferred to a freezing warehouse for storage. The freezing temperature is maintained at 0°C. During the storage period, the survival of the composite bacteria is sampled and tested to ensure that the storage time can reach 3-15 days.

[0029] Step S9: Sampling and testing the short-term stored composite bacteria once every three days. If the survival rate of the composite bacteria is reduced, the composite bacteria balls that cannot be used in a short time will be crushed and bagged and placed in a freezing warehouse at -15°C to -30°C to ensure that the storage time can be extended to 30-90 days.

[0030] The above embodiments are only preferred embodiments of the present invention and are not limitations of the technical solutions of the present invention. Any technical solution that can be implemented on the basis of the above embodiments without creative work should be deemed to fall within the scope of protection of the patent of the present invention.

[0031] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a composite bacterial strain for decomposing municipal sludge, characterized in that: The method comprises the following steps: S1, preparing two symmetrical spherical shells by using degradable plant materials; S2, preparing a nutrient ball of the nutrient compound strain; S3, preparing a culturing chamber of the nutrient compound strain by using degradable plant materials; S4, combining the nutrient ball of the nutrient compound strain with the two symmetrical spherical shells; S5, drilling a hole extending from the outside of the curved spherical shell to the inside of the nutrient ball; S6, inserting the columnar protrusion of the culturing chamber of the nutrient ball into the inside of the nutrient ball along the hole; S7, after the assembly is completed, standing for 1-3 days, and observing the reproduction of the strain by using infrared sensing equipment; S8, when the temperature continues to drop during the reproduction of the strain, the nutrient compound strain can be frozen for short-term storage; S9, sampling and testing the survival of the nutrient compound strain, and crushing, bagging and freezing the nutrient compound strain balls for long-term storage.

2. The method for preparing a composite bacterial strain for decomposing municipal sludge according to claim 1, characterized in that: The step S1: collecting plant straws, leaves, shrub branches, etc., using a crusher and a grinder to process the collected materials into powder with a size of 0.1-0.5 mm in diameter, adding water and mixing until viscous, and using professional equipment to extrude the above raw materials to prepare two spherical shells, the size of the spherical shells The diameter is controlled to be 5-10 cm, and after the spherical shells are formed, they are placed in a ventilated and shaded place to dry, and one of the spherical shells is reserved for a nutrient diversion port.

3. The method for preparing a composite bacterial strain for decomposing municipal sludge according to claim 1, characterized in that: The step S2: using plant materials to wrap the nutrients required for the growth of the composite bacteria, the nutrients can be solid or liquid, a nutrient filling port with a tubular structure extends from the top of the composite bacteria nutrient ball, and the size of the composite bacteria nutrient ball is smaller than the size of the ball shell.

4. The method for preparing a composite bacterial strain for decomposing municipal sludge according to claim 1, characterized in that: Step S3: The composite bacteria culture chamber is divided into two parts, one part is the bacteria storage space, which is a spherical structure, and the other part is a columnar protruding tube, which is connected to the bacteria storage space. Nutrients are placed inside the columnar protruding tube to prevent the bacteria from gathering inside the composite bacteria nutrient ball for a short time. The bacteria are added after the overall assembly is completed.

5. The method for preparing a composite bacterial strain for decomposing municipal sludge according to claim 1, characterized in that: The step S4 comprises the following steps: firstly gluing the reserved nutrient diversion port of the spherical shell and the nutrient filling port together, and then gluing the two spherical shells together to form a closed space. The adhesive may be yellow mud, plant glue, etc. The nutrients are added after the overall assembly is completed. The composite bacteria nutrient ball and the spherical shell are fixed with yellow mud, plant glue, etc., and the foundation is left to stand for 3-5 hours after the foundation is fixed.

6. The method for preparing a composite bacterial strain for decomposing municipal sludge according to claim 1, characterized in that: The step S5: drilling holes from the outside of the preliminarily assembled spherical shell according to the size of the columnar protruding tube, and the holes are extended to the inside of the composite bacteria nutrient ball.

7. The method for preparing a composite bacterial strain for decomposing municipal sludge according to claim 1, characterized in that: The step S6: different bacterial strains are selected according to the actual situation of treating urban sludge, and the selected bacterial strains are respectively injected into the composite bacterial strain culture bin, and then the composite bacterial strain culture bin is assembled together with the spherical shell and the composite bacterial strain nutrient ball through a columnar protruding tube, and the connecting gaps are sealed with yellow mud, plant glue, etc., and non-anaerobic bacterial strains are selected without sealing.

8. The method for preparing a composite bacterial strain for decomposing municipal sludge according to claim 1, characterized in that: The step S7: after the assembly is completed, the composite bacterial species ball is placed in a constant temperature space, and the reproduction of the bacterial species and the fusion and symbiosis of multiple bacterial species are observed by infrared sensing equipment.

9. The method for preparing a composite bacterial strain for decomposing municipal sludge according to claim 1, characterized in that: Step S8: By monitoring the temperature distribution of the composite bacteria ball at multiple points, it is comprehensively judged whether the reproduction of the composite bacteria has reached its peak. When the activity of the composite bacteria decreases and the monitored temperature begins to decrease, the composite bacteria ball is transferred to a freezing warehouse for storage. The freezing temperature is maintained at 0°C. During the storage period, the survival of the composite bacteria is sampled and tested to ensure that the storage time can reach 3-15 days.

10. The method for preparing a composite bacterial strain for decomposing municipal sludge according to claim 1, characterized in that: Step S9: Sampling and testing the short-term stored composite bacteria once every three days. If the survival rate of the composite bacteria is reduced, the composite bacteria balls that cannot be used in a short time are crushed and bagged, and placed in a freezing warehouse at minus 15°C to minus 30°C to ensure that the storage time can be extended to 30-90 days.

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