Lipase mutants and uses thereof

Abstract

The application belongs to the field of biopharmacy and bioconversion, and particularly relates to a lipase mutant and application thereof. The lipase CALB mutant of the application is obtained by adopting single-point mutation or combined mutation on positions 40, 134, 154, 189, 278 and 281 of the amino acid sequence shown in SEQ ID NO. 1. The mutant greatly improves the stereoselectivity in catalyzing the production of (S)-3-cyclohexene-1-methyl formate from racemic 3-cyclohexene-1-methyl formate. Compared with the traditional chemical method for preparing (S)-3-cyclohexene-1-methyl formate, the lipase CALB mutant provided by the application has high stereoselectivity, mild reaction conditions, is friendly to the environment, has reduced requirements on equipment, greatly reduces the production cost, and shows a wide application prospect in industrial application.

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