Small automatic dip dyeing device for cell smear

Abstract

The invention relates to a small-sized automatic dip dyeing device for cell smears. The small-sized automatic dip dyeing device comprises a box body, a clear water tank, a sewage tank, a dyeing assembly and a moving assembly, the dyeing assembly comprises a slide rack, a dye liquor cylinder, a cleaning loop and an air drying cylinder; the slide frame is provided with a slide groove, and a slide can be vertically placed in the slide groove; the moving assembly comprises a lead screw module, a rotating motor module and a four-rod assembly, and the slide rack is fixedly connected with the four-rod assembly. Compared with the prior art, the device is small in size, can be placed in an operating room, and is low in dye consumption and high in dyeing efficiency and dyeing quality.

Description

Technical Field

[0001] The invention relates to the technical field of medical clinical testing, in particular to a small automatic staining device for cell smears. Background Art

[0002] On-site rapid assessment is a method for pathologists to quickly stain and observe specimens on site, and promptly provide feedback on whether the specimens meet the needs of pathological diagnosis. On-site rapid assessment reduces the number of biopsies and operation time, obtains effective specimens in a short time, avoids repeated biopsy injuries and long-term anesthesia, reduces adverse effects on patients, and has high clinical application value. The on-site rapid assessment method is to first fix the biopsy sample smeared on the slide in a fixative, soak it in Dif A solution for twenty to thirty seconds, then immerse the slide in Dif B solution for staining for about twenty to thirty seconds, then rinse with distilled water and dry, read the slide under a microscope, and quickly make a cytological diagnosis.

[0003] At present, most of the diagnostic interventional operations in domestic hospitals are performed manually. The existing slide staining devices take up a large space and cannot be placed in the operating room. They are not suitable for on-site rapid evaluation during diagnostic interventional operations. In addition, they mostly use drop staining technology, which has a low dye utilization rate.

[0004] Patent CN202410717133.4 discloses a fully automatic cell smear pipetting and dyeing machine. First, the cell specimen is extracted and sampled at the cell sample pipetting device, and the cell specimen is extracted into the cell smear device. At this time, the conveying device moves the cell smear device toward the centrifugal device, and the centrifugal device undertakes the cell smear device output by the cell sample pipetting device, and completes the centrifugal operation for the cell specimen in the cell smear device. After the centrifugal device completes the centrifugal operation and outputs the cell smear device, the conveying device moves the cell smear device to the dyeing device for targeted dyeing, thereby realizing the sampling, centrifugation and dyeing automation of the cell specimen in the cell smear device, replacing manual labor, improving the efficiency of cell centrifugal smear production, effectively preventing the reduction of cell activity, and effectively realizing the independent preparation of a single cell specimen and avoiding cross infection between multiple cell specimens. However, the device is large in size, the conveying mechanism structure is complex, and the dyeing efficiency cannot be improved. Summary of the invention

[0005] The purpose of the present invention is to overcome the above-mentioned defects of the prior art and provide a small automatic cell smear staining device, which is small in size, can be placed in an operating room, uses less dye, has high staining efficiency and staining quality.

[0006] The present invention provides a small automatic cell smear staining device, which is small in size and can be placed in an operating room to realize automatic slide staining during a biopsy process. The use of the device is conducive to reducing the amount of dye used, improving staining efficiency, and enhancing staining quality.

[0007] The present invention provides a small automatic cell smear staining device, comprising: a box body, a clean water tank, a dirty water tank, and a staining component and a moving component located inside the box body;

[0008] The staining assembly comprises: a slide rack, a dye tank, a cleaning circuit and an air drying tank; the slide rack is provided with a slide slot, which can vertically place a slide;

[0009] The moving assembly comprises: a lead screw module, a rotating motor module and a four-rod assembly, and the glass slide rack is fixedly connected to the four-rod assembly.

[0010] Furthermore, the screw module includes: a screw guide rail, a screw slider and a stepper motor; the screw guide rail is fixedly connected to the box body, the screw slider is arranged on the screw guide rail, the screw slider can slide along the screw guide rail, and the stepper motor drives the screw to achieve linear movement.

[0011] Furthermore, the four-rod assembly includes: a linear push rod module, a connecting rod, and a push rod turntable; the linear push rod module includes a linear push rod and a linear push rod motor, the linear push rod is slidably connected to the linear push rod motor to achieve the change of the length of the linear push rod module, the connecting rod is provided with a connecting rod rotating shaft, and the connecting rod rotating shaft is concentrically matched with a hole set at one end of the linear push rod to achieve rotation around the axis;

[0012] Furthermore, the rotating motor module includes: a rotating motor and gears, wherein the gears include a large gear and a small gear meshing with each other, and the small gear is fixedly connected to the rotating shaft of the rotating motor.

[0013] Furthermore, the linear push rod module is fixedly connected to the large gear and the push rod turntable respectively, the large gear and the push rod turntable are arranged on the lead screw slider, and the large gear and the push rod turntable can rotate on the lead screw slider.

[0014] Furthermore, a through hole is provided on the side of the box body through which the connecting rod of the four-bar assembly can extend;

[0015] The four-rod assembly has a first working position and a second working position; when the four-rod assembly is in the first working position, the connecting rod is in a horizontal position and extends from the through hole, and the slide rack for loading or removing slides is fixed on the connecting rod; when the four-rod assembly is in the second working position, the connecting rod is in a vertical position, the slide rack and the cylinder body are at the same horizontal height, and the screw slider of the screw module moves horizontally, so that the slide rack can be moved to the dye tank, the cleaning tank of the cleaning circuit, and the air drying tank respectively. The air drying tank, the cleaning tank, and the three dye tanks are arranged in sequence horizontally inside the box body.

[0016] Furthermore, the four-rod assembly is located in the first working position. After the glass slide rack is loaded, the linear push rod module is retracted to the shortest position, and the rotating motor is activated, so that the connecting rod is in a vertical position; the lead screw moves until it is aligned with the lower cylinder body, the rotating motor is activated, and the linear push rod module is extended at the same time, so that the four-rod assembly is located in the second working position, and the glass slide rack is vertically lowered to the working position; after completing the corresponding processing operation, the rotating motor is activated and the linear push rod module is shortened at the same time, so that the glass slide rack is vertically raised and leaves the cylinder body; it enters three dye tanks, cleaning tanks and air-drying tanks in turn, completes dyeing, cleaning and air-drying, and the glass slide processing is completed; the lower linear push rod module is extended, and the rotating motor is activated, so that the four-rod assembly is located in the first working position, and the glass slide is taken out.

[0017] Furthermore, the cleaning circuit includes: two peristaltic pumps, a hose and a cleaning tank; the cleaning tank is provided with a water inlet and a water outlet, one peristaltic pump connects the clean water tank and the water inlet through a hose, and the other peristaltic pump connects the sewage tank and the water outlet through a hose.

[0018] Furthermore, when the four-bar mechanism is in the second working position and the glass slide rack is placed inside the cleaning tank, the peristaltic pump connected to the water inlet works to push clean water from the clean water tank to the water inlet to rinse the glass slide. At the same time, the peristaltic pump connected to the water outlet works to push excess waste water into the sewage tank to prevent water from gathering in the cleaning tank.

[0019] Furthermore, the air drying cylinder includes: a fan and a wind shield, one side of the wind shield is fixedly connected to the fan, and the other side of the wind shield is provided with ventilation holes; when the four-bar mechanism is in the second working position and the glass slide rack is placed inside the air drying cylinder, the fan is started to dry the glass slide.

[0020] The working principle is:

[0021] The four-rod assembly is in a horizontal position and extends from the through hole, that is, it is in the first working position. The user puts the five slides to be processed into the slide rack and fixes them on the connecting rod, waiting for dyeing. The two linear push rod modules shrink to the shortest, and the rotating motor works, so that the connecting rod is in a vertical position. The lead screw moves until the slide rack is aligned with the dye tank below, the rotating motor works, and the two linear push rod modules extend at the same time, so that the four-rod assembly is in the second working position, so that the slide rack can be vertically lowered into the tank, so that the slide is immersed in the fixing liquid and fixed. The rotating motor works, and the two linear push rod modules shorten at the same time, so that the slide rack can be vertically raised and leave the cylinder. The same principle enters the two dye tanks, the cleaning tank and the air-drying tank in turn, and completes the subsequent dyeing of dye A and dye B, and the cleaning and air-drying of the slides. When the slide rack is in the cleaning tank, the two peristaltic pumps work. The peristaltic pump connected to the water inlet pushes clean water from the clean water tank to the water inlet to rinse the slides, and the peristaltic pump connected to the water outlet pushes excess waste water into the sewage tank to prevent water from gathering in the cleaning tank. When the slide rack is in the air drying tank, the fan starts to dry the slides, and the slide processing is completed. The linear push rod module below extends, and the rotating motor works, so that the four-rod assembly is in the first working position, and the slide is taken out.

[0022] Compared with the prior art, the present invention has the following advantages:

[0023] The invention has a small size and can be placed in an operating room. It uses less dye and has high dyeing efficiency and quality. The automatic slide dyeing is achieved through a four-rod assembly. It has a small size and accurate and efficient positioning. The clean water tank, sewage tank, dyeing assembly and mobile assembly cooperate to achieve efficient dyeing. At the same time, the number of dye tanks can be increased or decreased as needed, which is flexible. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 It is a schematic diagram of the structure of a small automatic staining device for cell smear;

[0025] Figure 2 It is a front view schematic diagram of a small automatic staining device for cell smear;

[0026] Figure 3 It is a schematic diagram of the structure of the moving components of the small automatic staining device for cell smear;

[0027] Figure 4 It is a front view schematic diagram of the first working position of the small automatic cell smear staining device;

[0028] Figure 5 It is a left schematic diagram of the first working position of the small automatic cell smear staining device;

[0029] Figure 6 It is a front view schematic diagram of the second working position of the small automatic cell smear staining device;

[0030] Figure 7 It is a schematic diagram of the cleaning circuit structure of a small automatic staining device for cell smear;

[0031] Figure 8 It is a schematic diagram of the cross-section structure of the cleaning tank of the small automatic staining device for cell smear;

[0032] Fig. 9 This is a schematic diagram of the air-drying cylinder structure of a small automatic staining device for cell smears.

[0033] Figure markings: 1-box, 11-through hole, 2-clean water tank, 3-sewage tank, 41-slide rack, 42-dye liquid tank, 43-cleaning circuit, 431-peristaltic pump, 432-hose, 433-cleaning tank, 434-water inlet, 435-water outlet, 44-air drying cylinder, 441-fan, 442-wind shield, 443-ventilation hole, 6-screw module, 61-screw slider, 62-screw guide rail, 63-stepping motor, 7-rotating motor module, 71-gear, 72-rotating motor, 8-four-bar assembly, 81-linear push rod module, 811-linear push rod, 812-linear push rod motor, 82-connecting rod, 821-connecting rod shaft, 83-push rod turntable. DETAILED DESCRIPTION

[0034] The present invention is described in detail below in conjunction with the accompanying drawings and specific embodiments. Component models, material names, connection structures, control methods, algorithms and other features not clearly described in this technical solution are all considered to be common technical features disclosed in the prior art.

[0035] Example 1

[0036] This embodiment provides a small automatic cell smear staining device, such as Figure 1 , 2 , 3, 7, 8, 9, comprising: a box body 1, a clean water tank 2, a sewage tank 3, and a dyeing component and a moving component located inside the box body 1;

[0037] The staining assembly includes: a slide rack 41, three dyeing liquid tanks 42, a cleaning circuit 43 and an air drying tank 44; the slide rack 41 is provided with five slide slots, which can vertically place five slides;

[0038] The moving assembly includes: a lead screw module 6, a rotating motor module 7 and a four-bar assembly 8, and the glass slide rack 41 is fixedly connected to the four-bar assembly 8. The glass slide rack 41 is fixedly connected to the connecting rod 82 of the four-bar assembly 8.

[0039] In a specific embodiment, the screw module 6 includes: a screw guide rail 62, a screw slider 61 and a stepper motor 63; the screw guide rail 62 is fixedly connected to the box body 1, the screw slider 61 is arranged on the screw guide rail 62, the screw slider 61 can slide along the screw guide rail 62, and the stepper motor 63 drives the screw to achieve linear movement.

[0040] In a specific embodiment, the four-rod assembly 8 includes: two linear push rod modules 81, a connecting rod 82, and a push rod rotating disk 83; the linear push rod module 81 includes a linear push rod 811 and a linear push rod motor 812, the linear push rod 811 is slidably connected to the linear push rod motor 812 to achieve the change of the length of the linear push rod module 81, and the connecting rod 82 is provided with two connecting rod rotating shafts 821, and the connecting rod rotating shafts 821 are concentrically matched with the holes set at one end of the linear push rod 811 to achieve rotation around the axis;

[0041] In a specific implementation, the rotary motor module 7 includes: a rotary motor 72 and a gear 71 . The gear includes a large gear and a small gear meshing with each other. The small gear is fixedly connected to the rotating shaft of the rotary motor 72 .

[0042] In a specific embodiment, the two linear push rod modules 81 are respectively fixedly connected to the large gear and the push rod turntable 83 , and the large gear and the push rod turntable 83 are arranged on the screw slider 61 , and the large gear and the push rod turntable 83 can rotate on the screw slider 61 .

[0043] In a specific embodiment, a through hole 11 is provided on the side of the box body 1 for the connecting rod 82 of the four-bar assembly 8 to extend out;

[0044] like Figure 4 , 5 6, the four-rod assembly 8 has a first working position and a second working position; when the four-rod assembly 8 is in the first working position, the connecting rod 82 is in a horizontal position and extends from the through hole 11, and the glass slide rack 41 for loading or removing five glass slides is fixedly placed on the connecting rod 82; when the four-rod assembly 8 is in the second working position, the connecting rod 82 is in a vertical position, the glass slide rack 41 and the cylinder body are at the same horizontal height, and the screw slider 61 of the screw module 6 moves horizontally, so that the glass slide rack 41 can be moved to the two dyeing liquid cylinders 42, the cleaning cylinder 433 of the cleaning circuit 43, and the air drying cylinder 44 respectively. The air drying cylinder 44, the cleaning cylinder 433, and the three dyeing liquid cylinders 42 are arranged in sequence in a horizontal direction inside the box body 1.

[0045] In a specific embodiment, the four-rod assembly 8 is located in the first working position. After the glass slide rack 41 is loaded, the two linear push rod modules 81 are retracted to the shortest, and the rotating motor 72 is activated, so that the connecting rod 82 is in a vertical position; the lead screw moves until it is aligned with the lower cylinder body, the rotating motor 72 is activated, and the two linear push rod modules 81 are extended at the same time, so that the four-rod assembly 8 is located in the second working position, and the glass slide rack 41 is vertically lowered to the working position; after completing the corresponding processing operation, the rotating motor 72 is activated and the two linear push rod modules 81 are shortened at the same time, so that the glass slide rack 41 is vertically raised and leaves the cylinder body; it enters the three dye liquid cylinders 42, the cleaning cylinder 433 and the air drying cylinder 44 in turn, completes dyeing, cleaning and air drying, and the glass slide processing is completed; the lower linear push rod module 81 is extended, and the rotating motor 72 is activated, so that the four-rod assembly 8 is located in the first working position, and the glass slide is taken out.

[0046] In a specific embodiment, the cleaning circuit 43 includes: two peristaltic pumps 431, a hose 432 and a cleaning tank 433; the cleaning tank 433 is provided with a water inlet 434 and a water outlet 435, one peristaltic pump 431 connects the clean water tank 2 and the water inlet 434 through the hose 432, and the other peristaltic pump 431 connects the dirty water tank 3 and the water outlet 435 through the hose 432.

[0047] In a specific embodiment, when the four-bar mechanism 8 is in the second working position and the slide rack 41 is placed inside the cleaning tank 433, the peristaltic pump 431 connected to the water inlet 434 works to push clean water from the clean water tank 2 to the water inlet 434 to rinse the slides. At the same time, the peristaltic pump 431 connected to the water outlet 435 works to push excess waste water into the sewage tank 3 to prevent water from gathering in the cleaning tank 433.

[0048] In a specific embodiment, the air-drying cylinder 44 includes: two fans 441 and a wind shield 442, one side of the wind shield 442 is fixedly connected to the fan 441, and the other side of the wind shield 442 is provided with a ventilation hole 443; when the four-bar mechanism 8 is in the second working position and the glass slide rack 41 is placed inside the air-drying cylinder 44, the fan 441 is started to dry the glass slide.

[0049] The working principle is:

[0050] The four-rod assembly 8 is in a horizontal position and extends from the through hole 11, that is, it is in the first working position. The user puts the five slides to be processed into the slide rack 41 and fixes them on the connecting rod 82, waiting for dyeing. The two linear push rod modules 81 shrink to the shortest, and the rotating motor 72 works, so that the connecting rod 82 is in a vertical position. The lead screw moves until the slide rack 41 is aligned with the dyeing liquid cylinder 42 below, the rotating motor 72 works, and the two linear push rod modules 81 extend at the same time, so that the four-rod assembly 8 is located in the second working position, so that the slide rack 41 is vertically lowered into the cylinder, so that the slide is immersed in the fixing liquid and fixed. The rotating motor 72 works, and the two linear push rod modules 81 are shortened at the same time, so that the slide rack 41 is vertically raised and leaves the cylinder body. The same principle enters the two dyeing liquid cylinders 42, the cleaning cylinder 433 and the air-drying cylinder 44 in sequence, and completes the subsequent dyeing of dye A and dye B, and the cleaning and air-drying of the slides. When the slide rack 41 is in the cleaning cylinder 433, the two peristaltic pumps 431 work. The peristaltic pump 431 connected to the water inlet 434 pushes clean water from the clean water tank 2 to the water inlet 434 to rinse the slides, and the peristaltic pump 431 connected to the water outlet 435 pushes excess waste water into the waste water tank 3 to prevent water from gathering in the cleaning cylinder 433. When the slide rack 41 is in the air drying cylinder 44, the fan 441 starts to dry the slides, and the slide processing is completed. The lower linear push rod module 81 extends, and the rotating motor 72 works, so that the four-bar assembly 8 is located in the first working position, and the slides are taken out.

[0051] Components not described in detail in this embodiment are all existing components that can be purchased through public channels.

[0052] The above description of the embodiments is to facilitate the understanding and use of the invention by those skilled in the art. It is obvious that those skilled in the art can easily make various modifications to these embodiments and apply the general principles described herein to other embodiments without creative work. Therefore, the present invention is not limited to the above embodiments, and improvements and modifications made by those skilled in the art based on the disclosure of the present invention without departing from the scope of the present invention should be within the scope of protection of the present invention.

Claims

1. A small automatic cell smear staining device, characterized in that: include: A box body (1), a clean water tank (2), a sewage tank (3), and a dyeing component and a moving component located inside the box body (1); The staining assembly comprises: a slide rack (41), a dye tank (42), a cleaning circuit (43) and an air drying tank (44); the slide rack (41) is provided with a slide slot, which can vertically place a slide; The moving assembly comprises: a lead screw module (6), a rotating motor module (7) and a four-rod assembly (8), and the glass slide rack (41) is fixedly connected to the four-rod assembly (8).

2. A small automatic cell smear staining device according to claim 1, characterized in that: The lead screw module (6) comprises: a lead screw guide rail (62), a lead screw slider (61) and a stepping motor (63); the lead screw guide rail (62) is fixedly connected to the box body (1), the lead screw slider (61) is arranged on the lead screw guide rail (62), the lead screw slider (61) can slide along the lead screw guide rail (62), and the stepping motor (63) drives the lead screw to achieve linear movement.

3. A small automatic cell smear staining device according to claim 1, characterized in that: The four-rod assembly (8) comprises: a linear push rod module (81), a connecting rod (82), and a push rod rotating disk (83); the linear push rod module (81) comprises a linear push rod (811) and a linear push rod motor (812); the linear push rod (811) is slidably connected to the linear push rod motor (812) to achieve the change of the length of the linear push rod module (81); the connecting rod (82) is provided with a connecting rod rotating shaft (821); the connecting rod rotating shaft (821) is coaxially matched with a hole provided at one end of the linear push rod (811) to achieve rotation around the axis.

4. A small automatic cell smear staining device according to claim 3, characterized in that: The rotary motor module (7) comprises a rotary motor (72) and a gear (71), wherein the gear comprises a large gear and a small gear meshing with each other, and the small gear is fixedly connected to the rotating shaft of the rotary motor (72).

5. A small automatic cell smear staining device according to claim 4, characterized in that: The linear push rod module (81) is respectively fixedly connected to the large gear and the push rod rotating disk (83); the large gear and the push rod rotating disk (83) are arranged on the lead screw slider (61); the large gear and the push rod rotating disk (83) can rotate on the lead screw slider (61).

6. A small automatic cell smear staining device according to claim 1, characterized in that: A through hole (11) is provided on the side of the box body (1) for allowing the connecting rod (82) of the four-rod assembly (8) to extend out; The four-rod assembly (8) has a first working position and a second working position; when the four-rod assembly (8) is in the first working position, the connecting rod (82) is in a horizontal position and extends from the through hole (11), and a glass slide rack (41) for loading or removing glass slides is fixedly placed on the connecting rod (82); when the four-rod assembly (8) is in the second working position, the connecting rod (82) is in a vertical position, and the glass slide rack (41) and the cylinder body are at the same horizontal height, and the screw slider (61) of the screw module (6) moves horizontally, so that the glass slide rack (41) can be moved into the dye liquid cylinder (42), the cleaning cylinder (433) of the cleaning circuit (43), and the air drying cylinder (44) respectively.

7. A small automatic cell smear staining device according to claim 1, characterized in that: The four-rod assembly (8) is located at the first working position. After the glass slide rack (41) is loaded, the linear push rod module (81) is retracted to the shortest position, and the rotating motor (72) is operated, so that the connecting rod (82) is in a vertical position; the lead screw moves until it is aligned with the lower cylinder body, the rotating motor (72) is operated, and the linear push rod module (81) is extended at the same time, so that the four-rod assembly (8) is located at the second working position, and the glass slide rack (41) is vertically lowered to the working position; after the corresponding processing operation is completed, the rotating motor (72) is operated and the linear push rod module (81) is shortened, so that the glass slide rack (41) is vertically raised and leaves the cylinder body; it enters the three dyeing liquid cylinders (42), the cleaning cylinder (433) and the air drying cylinder (44) in sequence, and the dyeing, cleaning and air drying are completed, and the glass slide processing is completed; the lower linear push rod module (81) is extended, and the rotating motor (72) is operated, so that the four-rod assembly (8) is located at the first working position, and the glass slide is taken out.

8. A small automatic cell smear staining device according to claim 1, characterized in that: The cleaning circuit (43) comprises: two peristaltic pumps (431), a hose (432) and a cleaning tank (433); the cleaning tank (433) is provided with a water inlet (434) and a water outlet (435); one peristaltic pump (431) connects the clean water tank (2) and the water inlet (434) via the hose (432); and the other peristaltic pump (431) connects the dirty water tank (3) and the water outlet (435) via the hose (432).

9. A small automatic cell smear staining device according to claim 1, characterized in that: When the four-bar mechanism (8) is in the second working position and the glass slide rack (41) is placed inside the cleaning tank (433), the peristaltic pump (431) connected to the water inlet (434) works to push clean water from the clean water tank (2) to the water inlet (434) to rinse the glass slide. At the same time, the peristaltic pump (431) connected to the water outlet (435) works to push excess waste water into the sewage tank (3) to prevent water from gathering in the cleaning tank (433).

10. A small automatic cell smear staining device according to claim 1, characterized in that: The air drying cylinder (44) comprises: a fan (441) and a wind shield (442); one side of the wind shield (442) is fixedly connected to the fan (441), and the other side of the wind shield (442) is provided with a ventilation hole (443); when the four-bar mechanism (8) is in the second working position and the glass slide rack (41) is placed inside the air drying cylinder (44), the fan (441) is started to dry the glass slide.

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