Spectrophotometer for chemical analysis experiment
By using separation modules and delay modules to process optical signals in the spectrophotometer, the absorption peak steps and measurement error problems caused by light source switching are solved, and the accuracy and aesthetics of measurement are improved.
Patent Information
- Application Number
- CN202510318777.0
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-18
- Publication Date
- 2025-04-25
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
Existing spectrophotometers may cause absorption peak steps, measurement errors and aesthetic effects when switching light sources.
By introducing a separation module and a delay module into the spectrophotometer, the monochromatic light is divided into two beams, and one of the beams is delayed before the light source switch is switched to ensure the continuity and stability of the light signal during the light source switch.
It effectively avoids the light intensity change caused by light source switching, improves the accuracy and aesthetics of measurement, and ensures the smoothness of the absorption peak.
Smart Images
Figure CN119880833A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of optical detection, in particular to a spectrophotometer used in chemical analysis experiments. Background Art
[0002] As an analytical instrument widely used in the fields of chemistry, biology, medicine, environmental science, etc., spectrophotometer is mainly used to measure the absorption characteristics of substances in the ultraviolet, visible and near-infrared spectral regions. Its basic principle is based on the Lambert-Beer law, that is, the proportion of light absorbed by the transparent medium is independent of the intensity of the incident light, but is proportional to the thickness of the absorption layer, the concentration of the solution and the light absorption rate. This principle enables the spectrophotometer to achieve qualitative and quantitative analysis of substances.
[0003] The detection process of existing spectrophotometers usually includes the following steps: Before the detection, it is usually necessary to preheat the spectrophotometer for a period of time (such as 20 minutes) to ensure that the internal components of the instrument reach a stable state; prepare a sample solution of appropriate concentration according to the experimental requirements; place the sample in a cuvette and adjust the liquid level in the sample cell to a suitable height, usually requiring the liquid surface to be parallel to the sample cell window. At the same time, the selection of the cuvette should be based on the properties of the sample and the measurement wavelength, and the cuvette should be kept clean and dry; according to the experimental design, set the required measurement wavelength; place the cuvette in the sample chamber of the instrument and close the sample chamber cover; after placing the sample to be tested in the sample cell, set the measurement wavelength, integration time and other parameters, start measuring, and record its absorbance value; after the experiment, clean the sample cell and dry it for next use.
[0004] In current spectrophotometer technology, ultraviolet light measurement and visible light measurement usually need to switch to different light sources. Hydrogen lamps or deuterium lamps are generally used in the ultraviolet region, while tungsten lamps are used in the visible region. During the light source switching process, due to the different spectral characteristics and light intensities of different light sources, a sudden change in light intensity may occur near the switching point, thus forming a step on the absorption peak curve, affecting the accuracy of the measurement results and the aesthetics of the absorption peak.
[0005] In view of the defects of the existing technology, a new type of spectrophotometer is urgently needed to solve the problems of absorption peak steps, measurement errors and aesthetics that may be caused by light source switching. Summary of the invention
[0006] In order to solve the above problems, the purpose of the present invention is to provide a spectrophotometer for chemical analysis experiments, which can enhance the stability of the light source by optimizing the light source switching mechanism, thereby reducing the impact of the light source switching on the absorption peak measurement, thereby improving the accuracy and aesthetics of the measurement.
[0007] In order to achieve the above object, the technical solution of the present invention is as follows: A spectrophotometer for chemical analysis experiments comprises a housing, wherein a sample chamber, a light source module, a monochromator, a separation module, a delay module, a detection module and an integration module are arranged in the housing; The sample chamber is used to place the sample to be tested; The light source module is used to provide emitted light from different light sources; The monochromator is used to separate monochromatic light of target wavelength from the emitted light; The separation module is used to separate the monochromatic light into two beams before switching the light source, wherein one beam of monochromatic light is directed to the delay module, and the other beam of monochromatic light is directly directed to the sample chamber; The delay module is used to receive monochromatic light and extend the propagation time of the monochromatic light; The detection module is used to convert two beams of monochromatic light into electrical signals respectively, and draw first absorption spectrum curves respectively; The integration module is used to fit the first absorption spectrum curve acquired by the detection module to obtain a complete second absorption spectrum curve.
[0008] The above scheme has the following beneficial effects: 1. This solution cleverly divides the monochromatic light into two beams through the separation module, and uses the delay module to delay one of the beams before switching the light source. This design ensures that in the brief moment of light source switching, there is still a continuous light signal that is stably provided to the detection module, thereby effectively avoiding the sudden change of light intensity caused by light source switching and ensuring the continuity and stability of the measurement.
[0009] 2. This solution optimizes the light source switching mechanism to make the light source switching process smoother and more continuous, which helps to eliminate the absorption peak steps caused by light source switching and further improves the accuracy of measurement.
[0010] 3. This solution, by integrating the module to fit the first absorption spectrum curve of the two monochromatic lights obtained by the detection module, effectively avoids the influence of light source switching on the absorption peak measurement, and thus can more accurately reflect the light absorption characteristics of the sample to be tested. At the same time, the stable light source and continuous light signal also make the measured second absorption spectrum curve smoother and more beautiful, improving the readability and analysis efficiency of the data.
[0011] Furthermore, it also includes a rinsing module; the rinsing module includes a rinsing tank; the rinsing tank is arranged on the top of the shell, and a wiping component, a clamping component, a detection component and a cleaning component are arranged in the rinsing tank. The wiping component is used to perform unidirectional wiping on both sides of the cuvette after rinsing, the clamping component is used to fix the cuvette, the detection component is used to respectively detect the changes in the concentration of interfering components in the rinsing tank before and after rinsing, and the cleaning component is used to rinse the cuvette; the bottom of the rinsing tank is also connected to a waste liquid collection mechanism.
[0012] Beneficial effect: The cleaning component in the rinsing module can effectively rinse the cuvette to remove impurities remaining on the inner wall of the cuvette or the solution left over from the previous measurement, thereby avoiding the influence of these impurities or solutions on the current measurement results and improving the accuracy of the measurement.
[0013] Furthermore, the wiping assembly includes a pair of wiping rollers; the pair of wiping rollers are located at the top of the inner wall of the rinsing tank, and the pair of wiping rollers are divided into a fine inner diameter area and a coarse inner diameter area. The fine inner diameter area is used for the passage of the cuvette before rinsing begins, and the coarse inner diameter area is used for unidirectional wiping of both sides of the cuvette after rinsing is completed.
[0014] Beneficial effect: The design of the wiping rollers allows the cuvette to be effectively wiped when passing through. The design of the thin inner diameter area allows the cuvette to pass smoothly before the start of rinsing, so that the cuvette will not be subjected to excessive friction when passing through, thereby avoiding scratches or damage caused by friction.
[0015] The thick inner diameter area is used for unidirectional wiping of both sides of the cuvette after rinsing, that is, the cuvette can be wiped in one direction by simply pulling it out of the thick inner diameter area. This design not only ensures uniform wiping, but also improves wiping efficiency, allowing the cuvette to dry quickly and remove residual water or solution.
[0016] The introduction of the wiping roller makes the rinsing process smoother and more efficient. After rinsing, the cuvette can be wiped directly by the wiping roller without additional operation steps, thus simplifying the operation process and improving experimental efficiency.
[0017] Furthermore, the clamping assembly comprises a pair of clamping rollers, and the pair of clamping rollers are used to fix the inverted cuvette in the rinse tank.
[0018] Beneficial effects: The design of the clamping rollers can firmly clamp the inverted cuvette, keeping it stable and motionless during the rinsing process. This stable clamping ensures that the rinsing liquid can fully contact and clean the inner wall of the cuvette, thereby improving the rinsing effect. The introduction of the clamping rollers simplifies the operation process during the rinsing process. The operator only needs to invert the cuvette and place it in the rinsing tank, and the clamping rollers can automatically clamp it and fix it.
[0019] Furthermore, the detection component includes a plurality of electrodes, and each of the electrodes is electrically connected to an electrochemical measurement system.
[0020] Beneficial effects: The electrode can monitor the changes in the electrochemical properties of the solution in the rinse tank in real time, such as conductivity, pH value, etc., thereby indirectly reflecting the rinse effect. Through the electrochemical measurement system, the concentration changes of interfering components in the solution before and after rinsing can be accurately measured, so as to more accurately evaluate the rinse effect. This helps to eliminate measurement errors caused by solution residues and improve the accuracy of experimental data.
[0021] Furthermore, the cleaning component includes an injection tube and an ultrasonic generator; the injection tube is connected to an injection pump, and the injection pump is used to inject cleaning liquid into the rinse tank; the ultrasonic generator is used to generate ultrasonic waves to clean the colorimetric dish.
[0022] Beneficial effect: The injection pump injects cleaning liquid into the rinse tank through the injection pipe, and works together with ultrasonic waves to achieve efficient cleaning of the contrast color dish.
[0023] Furthermore, the waste liquid collecting mechanism comprises a waste liquid tank, the waste liquid tank is connected with the rinsing tank, and a valve is arranged on the communication path between the waste liquid tank and the rinsing tank.
[0024] Beneficial effect: The connection design between the waste liquid tank and the rinse tank allows the waste liquid to flow into the waste liquid tank automatically without the need for manual transfer by the operator. This not only simplifies the experimental process, but also improves the experimental efficiency.
[0025] Furthermore, the cleaning liquid is deionized water.
[0026] Beneficial effects: Deionized water can be used as an electrolyte in electrochemical measurements due to its low ion content and low conductivity. When deionized water is used as a cleaning solution, the concentration changes of interfering components in the electrolyte (i.e., the cleaning solution and the residue on the cuvette) before and after rinsing will be more obvious. This is because there are almost no other ions in deionized water, so any residual interfering components will significantly affect the conductivity or other electrochemical properties of the electrolyte, making it easier to be detected by the electrochemical measurement system.
[0027] Using deionized water as a cleaning solution helps reduce background noise in the electrolyte. Since the ion content in deionized water is extremely low, when using an electrochemical measurement system to detect the concentration of interfering components, the actual interfering components can be measured more accurately without being interfered by background noise.
[0028] Furthermore, it also includes an optimization module; the optimization module is used to obtain the timing characteristics in the sample detection process, the timing characteristics include a first absorption spectrum curve, a second absorption spectrum curve, a delay duration and light source parameters, the delay duration is the propagation duration of the monochromatic light extended by the extension module, and the residual algorithm is used to predict the first absorption spectrum curves of the two monochromatic lights respectively, based on the predicted first absorption spectrum curve and the complete second absorption spectrum curve. The smoothness and accuracy of the second absorption spectrum curve at the light source switching point are identified and adjusted, the smoothness and accuracy level of the current light source switching are evaluated, and a dynamic strategy for adjusting the delay duration for the next test is designed.
[0029] Beneficial effects: The optimization module uses a residual algorithm to predict the first absorption spectrum curves of two monochromatic lights. This prediction method can more accurately estimate the change trend of the absorption spectrum curve based on historical data and current status, thereby improving the accuracy of the prediction.
[0030] By comparing the predicted first absorption spectrum curve with the complete second absorption spectrum curve, the optimization module can more accurately identify the smoothness and accuracy issues of the absorption spectrum curve at the light source switching point. On this basis, targeted parameter adjustments can ensure that the light source switching process is smoother and more accurate.
[0031] After evaluating the smoothness and accuracy of the current light source switching, the optimization module can design a more reasonable delay adjustment strategy for the next test. This strategy not only takes into account the current performance, but also combines historical data and future trends, making the adjustment more intelligent and dynamic.
[0032] Further, the dynamic strategy design includes the following steps: comparing the second absorption spectrum curve monitored in real time with preset smoothness and accuracy standards; According to the comparison results, if the smoothness or accuracy of the light source switching point does not meet the requirements, the delay time is initially adjusted; If the curve change of the light source switching point is higher than the preset value, the delay time is increased; If the curve change of the light source switching point is lower than the preset value, the delay time is reduced.
[0033] Beneficial effect: By dynamically adjusting the delay time, the smooth transition of the absorption spectrum curve during the light source switching process can be ensured, reducing the occurrence of mutations and jitter.
[0034] When the curve of the light source switching point is monitored to change too dramatically, with obvious mutations or jitters, it means that the smoothness is insufficient. At this time, increasing the delay time can allow the system to obtain more first absorption spectrum curve data for a smooth transition, thereby improving smoothness and accuracy.
[0035] When it is detected that the curve change of the light source switching point is smooth enough without obvious mutation or jitter, the response speed is improved by reducing the delay time. BRIEF DESCRIPTION OF THE DRAWINGS
[0036] Figure 1 The three-dimensional structure schematic diagram of a spectrophotometer used for chemical analysis experiments of the present invention.
[0037] Figure 2 The present invention is a light path diagram of a spectrophotometer used in chemical analysis experiments.
[0038] Figure 3The present invention is a schematic structural diagram of a rinsing module in a spectrophotometer used in chemical analysis experiments.
[0039] The figure marks in the drawings of the specification include: 1. shell; 2. rinse tank; 3. liquid injection tube; 4. wiping roller; 5. electrode; 6. clamping roller; 7. cuvette; 8. ultrasonic generator; 9. waste liquid tank; 10. valve; 101. tungsten lamp; 102. deuterium lamp; 103. light source module; 104. monochromator; 105. beam splitter; 106. reflector; 107. delay device; 108. sample chamber; 109. detection module. DETAILED DESCRIPTION
[0040] Embodiments of the present invention are described in detail below, examples of which are shown in the accompanying drawings, wherein the same or similar reference numerals throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention.
[0041] In the description of the present invention, it is to be understood that the terms “longitudinal”, “lateral”, “vertical”, “up”, “down”, “front”, “back”, “left”, “right”, “vertical”, “horizontal”, “top”, “bottom”, “inside” and “outside” etc., indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.
[0042] In the description of the present invention, unless otherwise specified and limited, it should be noted that the terms "installed", "connected" and "connected" should be understood in a broad sense. For example, it can be a mechanical connection or an electrical connection, or it can be the internal connection between two components. It can be a direct connection or an indirect connection through an intermediate medium. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to the specific circumstances.
[0043] The following is further described in detail through specific implementation methods: Embodiment 1 is basically as attached Figure 1-Figure 3 As shown: a spectrophotometer for chemical analysis experiments, mainly comprising a housing 1, in which a sample chamber 108, a light source module 103, a monochromator 104, a separation module, a delay module, a detection module 109 and an integration module are mainly arranged.
[0044] The sample chamber 108 is used to place the sample to be tested. Figure 2As shown, the sample chamber 108 is placed before the detection module 109, specifically between the delay module, the separation module and the detection module 109. Specifically, the sample chamber 108 is a rectangular box, the interior of which is painted black to minimize light reflection and scattering. The sample chamber 108 generally includes two external holes and two internal holes to allow light to be introduced and released.
[0045] The light source module 103 is used to provide light emitted from different light sources. Preferably, the light source module 103 includes a tungsten lamp 101 light source, a deuterium lamp 102 light source and a concave mirror (including a lamp holder and a driving member, the driving member is a motor). The purpose of the concave mirror is to image the light emitted by the light source module 103 on the incident slit of the monochromator 104. Generally, the deuterium lamp 102 and the tungsten lamp 101 are located on the same side of the concave mirror, and the switching of the light source is achieved by driving the concave mirror to move by a motor (this part is the prior art and will not be described in detail).
[0046] The monochromator 104 is used to separate monochromatic light of a target wavelength from the emitted light. Specifically, the monochromator 104 mainly consists of an incident slit, a collimator (a lens or a concave reflector 106 that makes the incident light parallel), a dispersion element (such as a prism or a grating), a focusing element, and an exit slit.
[0047] The separation module is used to separate the monochromatic light into two beams before switching the light source, wherein one beam of monochromatic light is directed to the delay module, and the other beam of monochromatic light is directed directly to the sample chamber 108. Specifically, the separation module is essentially a beam splitter 105, which is made of two triangular glass prisms, which are glued together on a substrate using polyester, epoxy resin or polyurethane adhesive. When light is incident on the beam splitter 105, part of the light will be reflected and the other part of the light will be transmitted. In this embodiment, the reflected light will pass through the reflector 106 and be directed to the delay module, while the transmitted light will pass through the sample chamber 108 and be received by the detection module 109.
[0048] The delay module is used to receive monochromatic light and extend the propagation time of monochromatic light. Specifically, the delay module is a delay device 107 that can realize continuous adjustment of the propagation time of the optical signal. Preferably, the delay device 107 adopts a fully solid-state waveguide chip design, and its core structure includes key components such as input waveguide, direct-through end, upload end, download end, optical resonant cavity and tuning electrode.
[0049] When the optical signal enters from the input waveguide, it will go through a series of complex coupling and interference processes, and finally form a stable resonant mode in the optical resonant cavity. By adjusting the voltage on the tuning electrode, the refractive index of the optical resonant cavity is changed, thereby achieving precise control of the optical signal delay time.
[0050] The detection module 109 is used to convert the two monochromatic light beams into electrical signals respectively and draw the first absorption spectrum curves respectively. Preferably, the detection module 109 includes a detector and a signal processor. The detector is a photomultiplier tube, and the signal processor is used to process the electrical signal output by the detector and convert it into a digital signal or an analog signal for further analysis and processing.
[0051] The integration module is used to fit the first absorption spectrum curve acquired by the detection module 109 to obtain a complete second absorption spectrum curve.
[0052] The integration module first receives the electrical signal data corresponding to the two monochromatic light beams from the detection module 109, that is, the original data of the first absorption spectrum curve. These data may contain interference factors such as noise and baseline drift, so they need to be pre-processed.
[0053] The raw data is cleaned using digital signal processing (DSP) techniques, such as filtering, smoothing, baseline correction and other algorithms, to eliminate the influence of noise and baseline drift and obtain more accurate first absorption spectrum curve data.
[0054] The first absorption spectrum curves corresponding to the two pre-processed monochromatic light beams are fitted to obtain a complete second absorption spectrum curve. The first absorption spectrum curves of the two monochromatic light beams are fitted using nonlinear fitting algorithms, such as Gaussian fitting and Lorentz fitting. At the same time, according to the delay information provided by the delay module, the spectrum curves of the two light beams are time-aligned and intensity-corrected to obtain an accurate second absorption spectrum curve.
[0055] Preferably, the second absorption spectrum curve is analyzed using chemometric methods, such as multiple linear regression, principal component regression, neural network and other algorithms. Based on the analysis results, the components present in the sample and their concentrations can be determined, or the purity of the sample can be evaluated.
[0056] The difference between the second embodiment and the first embodiment is that it also includes a rinse module; Figure 3 As shown, the rinsing module includes a rinsing tank 2; the rinsing tank 2 is opened on the top of the shell 1, and a wiping component, a clamping component, a detection component and a cleaning component are arranged in the rinsing tank 2.
[0057] The wiping assembly is used to perform unidirectional wiping on both sides of the rinsed cuvette 7 .
[0058] Specifically, the wiping assembly includes a wiping roller pair 4; the wiping roller pair 4 is located at the top of the inner wall of the rinse tank 2, that is, at the opening of the rinse tank 2, and both ends of the wiping roller pair 4 are rotatably connected to the inner wall of the rinse tank 2; the wiping roller pair 4 is divided into a thin inner diameter section and a thick inner diameter section. Figure 3The left side of the wiping roller 4 is a thick inner diameter section, and the gap area between the thick inner diameter sections is the thick inner diameter area. The right side of the wiping roller 4 is a thin inner diameter section, and the gap area between the thin inner diameter sections is the thin inner diameter area. The thin inner diameter area is used for the passage of the cuvette 7 before rinsing, and the thick inner diameter area is used for unidirectional wiping of both sides of the cuvette 7 after rinsing. Preferably, in this embodiment, the wiping roller 4 is divided into two halves by its center line, and the thin inner diameter area is used for wiping the cuvette 7 after rinsing. Figure 3 As shown, the left side is the thick inner diameter area, and the right side is the thin inner diameter area. When the cleaning is completed, the cuvette 7 is pulled out from the thick inner diameter area, and the two sides of the cuvette 7 (specifically, the two sides where the light is horizontally irradiated) can be wiped unidirectionally, and the one-way wiping can avoid scratches and other interference factors on the two sides of the cuvette 7.
[0059] The clamping assembly is used to fix the cuvette 7. Specifically, the clamping assembly includes a pair of clamping rollers 6, both ends of which are rotatably connected to the inner wall of the rinse tank 2, and the pair of clamping rollers 6 are used to fix the inverted cuvette 7 in the rinse tank 2.
[0060] The detection component is used to detect the concentration changes of interfering components in the rinse tank 2 before and after rinsing. Specifically, the detection component includes a plurality of electrodes 5, and the electrodes 5 are electrically connected to the electrochemical measurement system. Preferably, in this embodiment, the electrode 5 includes a working electrode, a counter electrode and a reference electrode, and the counter electrode and the working electrode are used to form a current loop to ensure that the current can smoothly pass through the rinse tank 2 (electrochemical tank). The reference electrode is used to provide a stable potential reference point to measure the potential change of the working electrode. The electrochemical measurement system performs quantitative analysis by utilizing the relationship between the potential of the working electrode and the ion activity in the solution in the rinse tank 2, so as to determine the concentration change of the interfering components (attached / residual on the cuvette 7) in the rinse tank 2 before and after rinsing.
[0061] The cleaning component is used to rinse the colorimetric dish 7. Specifically, the cleaning component includes an injection tube 3 and an ultrasonic generator 8; the injection tube 3 is connected to an injection pump, and specifically, the liquid outlet of the injection tube 3 is located on the side wall of the rinsing tank 2, and the injection pump is used to inject cleaning liquid into the rinsing tank 2. Preferably, the cleaning liquid is deionized water. The ultrasonic generator 8 is used to generate ultrasonic waves to clean the colorimetric dish 7. Preferably, in this embodiment, the ultrasonic generator 8 includes an ultrasonic transducer in addition to the generator body. The generator body is responsible for generating high-frequency electrical signals, and these signals will drive the ultrasonic transducer to work. The transducer uses a special piezoelectric ceramic material with good piezoelectric effect and mechanical strength. Driven by the high-frequency electrical signal, the piezoelectric ceramic material will produce expansion and contraction deformation, thereby driving the liquid in the rinsing tank 2 to produce high-frequency vibrations and form a dense ultrasonic beam.
[0062] The bottom of the rinse tank 2 is also connected to a waste liquid collection mechanism. Specifically, the waste liquid collection mechanism includes a waste liquid tank 9, which is connected to the rinse tank 2, and a valve 10 is welded and fixed on the communication path between the waste liquid tank 9 and the rinse tank 2.
[0063] Specific implementation steps: Initially, the cuvette 7 to be rinsed is inserted upside down from the thin inner diameter area, and then Figure 3 Move the center horizontally to the left, align between the clamping rollers 6, insert the cuvette 7 to be rinsed into the gap between the clamping rollers 6, and then deliver deionized water to the rinsing tank 2 through the injection pipe 3 to completely soak the cuvette 7. Then start the electrochemical measurement system to collect the concentration of the interfering components in the rinsing tank 2 before rinsing.
[0064] Then start the ultrasonic generator 8 to make the liquid in the rinse tank 2 generate high-frequency vibrations to form a dense ultrasonic beam. When the ultrasonic beam propagates in the liquid, it will produce cavitation, acceleration and straight-forward flow effects, which will strongly impact and peel off the inner and outer walls of the cuvette 7, effectively removing the residues attached to the cuvette 7.
[0065] When the rinsing reaches the predetermined time, open the valve 10 in the waste liquid collection mechanism to allow the waste liquid to flow from the rinsing tank 2 into the waste liquid tank 9. After ensuring that the waste liquid is completely discharged, close the valve 10. Then re-inject deionized water and use the electrochemical measurement system to detect the concentration of the interfering components in the rinsing tank 2 again (this time it is the concentration of the interfering components after rinsing). By the change in the concentration of the interfering components before and after rinsing, it is judged whether the cuvette 7 has been completely rinsed and no residue is attached. If it is not rinsed, repeat the above rinsing steps until the cuvette 7 is completely rinsed.
[0066] Then remove the rinsed cuvette 7 from the clamping roller 6 and Figure 3 The winning cuvette 7 is moved horizontally to the left and drawn out from the thick inner diameter area, and the two sides of the cuvette 7 are wiped unidirectionally by the wiping roller 4.
[0067] The only difference between Example 3 and the above-mentioned Example 2 is that it also includes an optimization module; the optimization module is used to obtain the timing characteristics in the sample detection process, and the timing characteristics include a first absorption spectrum curve, a second absorption spectrum curve, a delay duration and light source parameters, the delay duration is the propagation duration of the monochromatic light extended by the extension module, and the light source parameters are the wavelength and intensity of the monochromatic light. The residual algorithm is used to predict the first absorption spectrum curves of the two monochromatic lights respectively, and the predicted first absorption spectrum curve is compared with the complete second absorption spectrum curve to identify and adjust the smoothness and accuracy of the second absorption spectrum curve at the light source switching point, evaluate the smoothness and accuracy level of the current light source switching, and design a dynamic strategy for adjusting the delay duration for the next test.
[0068] The dynamic strategy design includes the following steps: comparing the second absorption spectrum curve monitored in real time with preset smoothness and accuracy standards; based on the comparison results, if the smoothness or accuracy of the light source switching point does not meet the requirements, preliminarily adjusting the delay time; if the curve change of the light source switching point is higher than the preset value, increasing the delay time; if the curve change of the light source switching point is lower than the preset value, reducing the delay time.
[0069] Specific implementation process: In this embodiment, 1 second before the light source is switched, the optimization module first obtains the first absorption spectrum curve (in this embodiment, denoted as ), and after the light source is switched, the first absorption spectrum curve of the monochromatic light captured by the detection module 109 and relayed by the delay module is obtained (in this embodiment, it is recorded as ), and then obtain the second absorption spectrum curve obtained by the integration module (in this embodiment, denoted as ).
[0070] Then, the residual algorithm is used to and Make a prediction and get and , and and Respectively Compare to identify and adjust the second absorption spectrum curve Smoothness and precision at light source switching points.
[0071] By comparing it with the preset smoothness and accuracy standards, it is determined whether the smoothness and accuracy meet the requirements, and the delay duration is preliminarily adjusted.
[0072] If the curve change (such as slope, fluctuation, etc.) at the light source switching point is higher than the preset value, it indicates that the smoothness is insufficient. At this time, the delay time should be increased (the refractive index of the optical resonant cavity should be changed) to improve the smoothness and accuracy. The specific principle is: when the propagation time of monochromatic light in the delay module increases, the detection module 109 can capture the spectral data in a longer period of time. This means that more data sampling points can be obtained near the light source switching point, and the detection module 109 can obtain The longer the wavelength, the more effective data can be obtained, and more data sampling points can help to more accurately describe the spectral changes during the light source switching process, especially when such changes are very subtle or complex. This helps to improve the smoothness and accuracy of the second absorption spectrum curve at the light source switching point.
[0073] If the curve change of the light source switching point is lower than the preset value, the delay time can be appropriately reduced to improve the response speed. The specific principle is: although increasing the delay time can improve smoothness and accuracy, too long a delay will lead to a slower response speed. Therefore, after preliminary adjustment, if the curve change of the light source switching point is smooth enough and the accuracy meets the requirements, then appropriately reducing the delay time can improve the response speed.
[0074] After the initial adjustment, the second absorption spectrum curve obtained from the next sample test is tested again to evaluate the adjustment effect. If it still does not meet the requirements, the delay time adjustment strategy is further optimized based on the feedback results.
[0075] The above is only an embodiment of the present invention, and the common knowledge such as the known specific structure and / or characteristics in the scheme is not described in detail here. It should be pointed out that for those skilled in the art, several deformations and improvements can be made without departing from the structure of the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicality of the patent. The scope of protection required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.
Claims
1. A spectrophotometer for chemical analysis experiments, comprising a housing (1), characterized in that: The housing (1) is provided with a sample chamber (108), a light source module (103), a monochromator (104), a separation module, a delay module, a detection module (109), and an integration module; The sample chamber (108) is used to place the sample to be tested; The light source module (103) is used to provide emitted light from different light sources; The monochromator (104) is used to separate monochromatic light of a target wavelength from the emitted light; The separation module is used to separate the monochromatic light into two beams before switching the light source, wherein one beam of monochromatic light is directed to the delay module and the other beam of monochromatic light is directed directly to the sample chamber (108); The delay module is used to receive monochromatic light and extend the propagation time of the monochromatic light; The detection module (109) is used to convert the two monochromatic light beams into electrical signals respectively, and draw first absorption spectrum curves respectively; The integration module is used to fit the first absorption spectrum curve acquired by the detection module (109) to obtain a complete second absorption spectrum curve.
2. The spectrophotometer for chemical analysis experiments according to claim 1, characterized in that: The invention also comprises a rinse module; the rinse module comprises a rinse tank (2); the rinse tank (2) is arranged at the top of the housing (1); a wiping assembly, a clamping assembly, a detection assembly and a cleaning assembly are arranged in the rinse tank (2); the wiping assembly is used to wipe both sides of the cuvette (7) after rinsing in one direction; the clamping assembly is used to fix the cuvette (7); the detection assembly is used to detect the concentration change of the interfering component in the rinse tank (2) before and after rinsing; and the cleaning assembly is used to rinse the cuvette (7); the bottom of the rinse tank (2) is also connected to a waste liquid collection mechanism.
3. The spectrophotometer for chemical analysis experiments according to claim 2, characterized in that: The wiping assembly comprises a pair of wiping rollers (4); the pair of wiping rollers (4) are located at the top of the inner wall of the rinsing tank (2); the pair of wiping rollers (4) are divided into a fine inner diameter area and a coarse inner diameter area; the fine inner diameter area is used for the passage of the cuvette (7) before rinsing begins; and the coarse inner diameter area is used for unidirectional wiping of both sides of the cuvette (7) after rinsing ends.
4. The spectrophotometer for chemical analysis experiments according to claim 3, characterized in that: The clamping assembly comprises a pair of clamping rollers (6), and the pair of clamping rollers (6) are used to fix the inverted cuvette (7) in the rinse tank (2).
5. The spectrophotometer for chemical analysis experiments according to claim 4, characterized in that: The detection component comprises a plurality of electrodes (5), and the electrodes (5) are all electrically connected to an electrochemical measurement system.
6. The spectrophotometer for chemical analysis experiments according to claim 5, characterized in that: The cleaning component comprises an injection pipe (3) and an ultrasonic generator (8); the injection pipe (3) is connected to an injection pump, and the injection pump is used to inject cleaning liquid into the rinse tank (2); the ultrasonic generator (8) is used to generate ultrasonic waves to clean the colorimetric dish (7).
7. The spectrophotometer for chemical analysis experiments according to claim 6, characterized in that: The waste liquid collection mechanism comprises a waste liquid tank (9), the waste liquid tank (9) and the rinse tank (2) are connected to each other, and a valve (10) is provided on the communication path between the waste liquid tank (9) and the rinse tank (2).
8. The spectrophotometer for chemical analysis experiments according to claim 7, characterized in that: The cleaning liquid is deionized water.
9. The spectrophotometer for chemical analysis experiments according to claim 8, characterized in that: It also includes an optimization module; the optimization module is used to obtain the timing characteristics in the sample detection process, the timing characteristics include a first absorption spectrum curve, a second absorption spectrum curve, a delay duration and light source parameters, the delay duration is the propagation duration of the monochromatic light extended by the extension module, and the first absorption spectrum curves of the two monochromatic lights are predicted respectively by using a residual algorithm, and the predicted first absorption spectrum curve is compared with the complete second absorption spectrum curve to identify and adjust the smoothness and accuracy of the second absorption spectrum curve at the light source switching point, evaluate the smoothness and accuracy level of the current light source switching, and design a dynamic strategy for adjusting the delay duration for the next test.
10. The spectrophotometer for chemical analysis experiments according to claim 9, characterized in that: The dynamic strategy design includes the following steps: comparing the second absorption spectrum curve monitored in real time with preset smoothness and accuracy standards; According to the comparison results, if the smoothness or accuracy of the light source switching point does not meet the requirements, the delay time is initially adjusted; If the curve change of the light source switching point is higher than the preset value, the delay time is increased; If the curve change of the light source switching point is lower than the preset value, the delay time is reduced.