Traditional Chinese medicine compound for treating hyperuricemia and capable of clearing heat, eliminating dampness and removing blood stasis and preparation method of traditional Chinese medicine compound

Abstract

The invention discloses a traditional Chinese medicine compound for treating hyperuricemia, clearing heat, eliminating dampness and removing stasis and a preparation method thereof, and belongs to the field of traditional Chinese medicine preparations for treating hyperuricemia diseases. The traditional Chinese medicine composition is prepared from 15-30 parts of herba lysimachiae, 15-30 parts of radix salviae miltiorrhizae, 10-20 parts of radix astragali, 10-20 parts of semen coicis, 5-15 parts of cortex magnoliae officinalis, 5-15 parts of plantain herb, 8-12 parts of rhizoma atractylodis macrocephalae, 8-10 parts of rhizoma acori graminei, 7-12 parts of rhizoma atractylodis, 9-12 parts of cortex phellodendri, 7-12 parts of herba leonuri The medicines are combined, so that the effects of the medicines generate a synergistic effect, and the effects of clearing heat, promoting diuresis, promoting blood circulation, removing blood stasis, removing turbidity and expelling toxins are achieved together. The whole compatibility has the effects of consolidating the root and eliminating evil, so that the body fluid is delivered and distributed smoothly, and the arthralgia syndrome is solved. The traditional Chinese medicine compound has the functions of clearing heat, eliminating dampness and promoting blood circulation to remove blood stasis, and has the advantages of short treatment course, quick effect, low possibility of relapse after healing, small side effect, low price and convenience in use.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine preparations for treating hyperuricemia, and specifically relates to a traditional Chinese medicine compound for clearing away heat, removing dampness and eliminating blood stasis for treating hyperuricemia and a preparation method thereof. Background Art

[0002] Hyperuricemia (HUA) is a metabolic syndrome characterized by purine metabolism disorder caused by excessive uric acid production or reduced excretion. Long-term illness can lead to gout and gouty arthritis, which can cause joint deformity and dysfunction. HUA has complex influencing factors and is often caused by acquired factors such as high-purine diet, excessive fructose and alcohol intake, and myeloproliferative diseases. The exact pathogenesis is not yet fully understood. Its overall prevalence is between 14.4% and 17.7%, and has shown a significant upward trend and a younger age trend in recent years.

[0003] HUA is a risk factor for many metabolic disorders, including hypertension, insulin resistance, dyslipidemia, atherosclerosis, non-alcoholic fatty liver disease, etc. In clinical practice, HUA patients often have no obvious symptoms in the early stages, and the public's awareness of HUA is generally low. Many people do not even know that this is a disease that needs treatment, which leads to the inability to detect and treat it in time, worsening of the disease, and an irreversible situation.

[0004] Currently, Western medicine is often used to treat this disease clinically, but the available drugs are limited, and first-line drugs have the risk of severe hepatotoxicity and new hepatotoxicity when treating HUA, which limits their safe use. More and more studies have shown that HUA is closely related to gout, the development of chronic kidney disease, and the increase of cardiovascular-related mortality and all-cause mortality. Therefore, it is necessary to further find new and safe drugs to treat HUA.

[0005] Traditional Chinese medicine does not record this disease name, but because HUA can cause gout and cause joint pain and discomfort, it is classified as "arthritis" and "lijie". Traditional Chinese medicine believes that this disease is due to external dampness invasion and improper diet that damages the spleen and kidneys. Therefore, starting from the holistic concept of traditional Chinese medicine, syndrome differentiation and treatment have unique therapeutic advantages for clinical patients to prevent and treat HUA. Summary of the invention

[0006] The purpose of the present invention is to provide a Chinese medicinal compound for clearing away heat, removing dampness and eliminating blood stasis for effectively treating hyperuricemia and a preparation method thereof, and to select effective drugs for treating hyperuricemia for clinical application through pharmacological and clinical research.

[0007] A Chinese medicinal compound for clearing away heat, removing dampness and eliminating blood stasis for treating hyperuricemia and a preparation method thereof, wherein the effective ingredients of the Chinese medicinal compound are prepared from the following Chinese medicinal raw materials in parts by weight:

[0008]

[0009]

[0010] In the heat-clearing, dampness-removing and blood-stasis-removing traditional Chinese medicine compound for treating hyperuricemia, the medicinal effects of each traditional Chinese medicine are as follows:

[0011] Herba Lysimachiae, also known as Herba Lysimachiae, ...

[0012] Danshen, also known as red root, Dahongpao, and Xueshen root, is bitter and slightly cold in nature. It enters the heart and liver meridians. It has the effects of promoting blood circulation and removing blood stasis, relieving pain through menstruation, clearing the heart and eliminating vexation, cooling blood and eliminating carbuncle. It is mainly used for amenorrhea and dysmenorrhea caused by blood stasis, abdominal pain due to postpartum blood stasis, pain in the heart and abdomen, accumulation of lumps, pain due to heat arthralgia, restlessness and insomnia. Modern pharmacological studies have shown that it has the effects of improving microcirculation, anti-inflammatory and antioxidant effects. Studies have found that the ingredients it contains, such as tanshinone and tanshinolic acid, can regulate the signal pathways related to uric acid metabolism and promote the transport and excretion of uric acid; it can also reduce the body's inflammatory response caused by hyperuricemia, and play a positive role in preventing and treating hyperuricemia and its complications by improving blood circulation.

[0013] Astragalus, also known as Mianqi, is sweet and slightly warm in nature. It enters the spleen and lung meridians. It has the effects of replenishing qi and strengthening the exterior, promoting diuresis and expelling toxins, draining pus, and healing sores and promoting tissue regeneration. It is mainly used for qi deficiency and fatigue, poor appetite and loose stools, sinking of the middle qi, chronic diarrhea and prolapse of the anus, bloody stools and metrorrhagia, spontaneous sweating due to exterior deficiency, etc. Modern pharmacological studies have shown that it has the effects of regulating the body's immune function, enhancing the filtration and excretion functions of the kidneys, and improving the body's overall metabolic state. Studies have found that it can enhance the filtration and excretion functions of the kidneys, help excrete metabolic wastes such as uric acid, and at the same time correct the metabolic disorders associated with hyperuricemia.

[0014] Coix seed, also known as coix seed, coix seed, etc., is cool in nature, sweet and light in taste. It enters the spleen, stomach, and lung meridians. It has the effects of promoting water and dampness, strengthening the spleen and stopping diarrhea, removing numbness, draining pus, detoxifying and dispersing knots. It is mainly used for edema, beriberi, dysuria, spleen deficiency diarrhea, dampness and stiffness, lung abscess, intestinal abscess, etc. Modern pharmacological studies have shown that it has the effects of regulating blood lipids, improving insulin resistance, and promoting water and dampness. Studies have found that ingredients such as coix seed polysaccharides in coix seed can regulate blood lipids and improve insulin resistance. Given that hyperuricemia is often associated with metabolic syndrome, it helps to improve the overall metabolic environment and indirectly affect the production and excretion of uric acid.

[0015] Magnolia officinalis, also known as thick bark, heavy bark, red bark, and fierce bark, tastes bitter and pungent, and is warm in nature. It enters the spleen, stomach, lung, and large intestine meridians. It has the effects of promoting qi and eliminating accumulation, drying dampness and eliminating fullness, and relieving asthma. It is mainly used for dampness stagnation, abdominal distension, vomiting and diarrhea, food accumulation and qi stagnation, abdominal distension and constipation, phlegm and cough, etc. Modern pharmacological studies have shown that it has anti-inflammatory, antioxidant and gastrointestinal function regulating effects. Studies have found that ingredients such as magnolol in Magnolia officinalis can reduce the production of endogenous uric acid by improving the digestion and absorption function of the gastrointestinal tract, and can regulate the body's metabolic rhythm, assist in maintaining the balance of uric acid metabolism, and play a certain auxiliary role in the treatment of hyperuricemia. It also helps to reduce the body's inflammatory response that may be associated with hyperuricemia.

[0016] Plantago, also known as shrimp toad clothes, cow's remains, and Shengxi, is sweet in taste and cold in nature. It enters the liver, kidney, lung, and small intestine meridians. It has the effects of clearing away heat, promoting diuresis, relieving stranguria, removing phlegm, cooling blood, and detoxifying. It is mainly used for painful stranguria caused by heat, edema and oliguria, diarrhea caused by summer heat and dampness, cough caused by phlegm and heat, vomiting blood, epistaxis, carbuncle, sore, etc. Modern pharmacological studies have shown that it has a significant diuretic effect and inhibits the reabsorption of uric acid by the renal tubules. Studies have found that it can increase urine volume, promote the excretion of uric acid with urine, and inhibit the reabsorption of uric acid by the renal tubules, further improve the excretion efficiency of uric acid, and play a positive role in lowering blood uric acid levels.

[0017] Atractylodes macrocephala, also known as Yushu, Dongshu, Zheshu, Shanji, Shanjing. It tastes bitter and sweet, and is warm in nature. It enters the spleen and stomach meridians and has the effects of replenishing qi and strengthening the spleen, drying dampness and promoting diuresis, stopping sweating, and calming the fetus. It is mainly used to treat spleen deficiency, poor appetite, abdominal distension and diarrhea, phlegm and fluid, palpitations, edema, spontaneous sweating, and restless fetuses. Modern pharmacological studies have shown that it has the effects of regulating intestinal flora, regulating immunity, anti-inflammatory, antioxidant, and improving metabolic disorders. Studies have found that Atractylodes lactone A, B and sugars (mainly mannose and fructose) can regulate intestinal flora and improve the intestinal microecological environment. Because intestinal flora is closely related to uric acid metabolism, it helps to inhibit the production of uric acid by regulating the balance of intestinal flora. At the same time, it can enhance the function of spleen and stomach transportation.

[0018] Acorus calamus, also known as calamus, nine-section calamus, etc., tastes pungent, bitter, and warm in nature. It enters the heart and stomach meridians. It has the effects of opening the orifices and eliminating phlegm, refreshing the mind and improving intelligence, and removing dampness and stimulating appetite. It is mainly used for coma, epilepsy, forgetfulness, insomnia, tinnitus, deafness, abdominal distension without hunger, and dysentery. Modern pharmacological studies have shown that it has antioxidant and anti-inflammatory effects and has a certain regulatory effect on the nervous system. Studies have found that the extract of Acorus calamus has antioxidant and anti-inflammatory effects, which can reduce the oxidative stress and inflammatory damage of the body caused by hyperuricemia, and its regulatory effect on the nervous system can also help improve some neurological dysfunctions that may be caused by hyperuricemia, and assist in the treatment of hyperuricemia from multiple angles.

[0019] Atractylodes, also known as red thuja, green thuja, etc., is pungent, bitter and warm in nature. It enters the spleen, stomach and liver meridians. It has the effects of drying dampness and strengthening the spleen, dispelling wind and cold, and improving eyesight. It is mainly used for dampness blocking the middle jiao, abdominal distension, diarrhea, edema, beriberi, rheumatic pain, cold, night blindness, etc. Modern pharmacological studies have shown that it has the effects of regulating gastrointestinal function, promoting the secretion of digestive juices and regulating body metabolism. Studies have found that Atractylodes contains volatile oils and other ingredients, which can regulate gastrointestinal function, promote the secretion of digestive juices, enhance the ability of the spleen and stomach to transport water and moisture, reduce the accumulation of moisture in the body, and thus reduce the related factors of uric acid production. At the same time, its regulatory effect on the body's metabolism is also conducive to maintaining the normal metabolic level of uric acid and assisting in improving the pathological state of hyperuricemia.

[0020] Phellodendron chinense, also known as berberine, phellodendron chinense, etc., is bitter and cold in nature. It enters the kidney and bladder meridians. It has the effects of clearing away heat and dampness, purging fire and detoxifying, and removing bone steaming. It is mainly used for damp-heat diarrhea, jaundice and red urine, vaginal itching, hot stranguria and pain, beriberi and paralysis, bone steaming and fatigue fever, night sweats, spermatorrhea, sores and swelling, eczema and wet sores, etc. Modern pharmacological studies have shown that it has anti-inflammatory, antibacterial and lipid metabolism regulating effects. Studies have found that berberine and other ingredients in Phellodendron chinense have anti-inflammatory, antibacterial and lipid metabolism regulating effects. It can reduce the inflammatory response associated with hyperuricemia through anti-inflammatory, and regulating lipid metabolism helps to improve the overall metabolic status and indirectly affect the metabolic process of uric acid. Moreover, its heat-clearing and damp-drying effects help to create an internal environment that is not conducive to uric acid deposition, which is conducive to the excretion of uric acid and the control of the disease.

[0021] Motherwort, also known as Cnidium monnieri and Kuncao, is pungent, bitter and slightly cold in nature. It enters the liver, pericardium and bladder meridians. It has the effects of promoting blood circulation and regulating menstruation, promoting diuresis and reducing swelling, clearing away heat and detoxifying. It is mainly used for irregular menstruation, dysmenorrhea, amenorrhea, lochia retention, edema and oliguria, sores and swelling, etc. Modern pharmacological studies have shown that it has the effects of improving microcirculation, increasing renal blood flow, and promoting blood circulation and removing blood stasis. Studies have found that motherwort can improve microcirculation, increase renal blood flow, and promote the filtration and excretion of uric acid by the kidneys. At the same time, its effect of promoting blood circulation and removing blood stasis can improve the hypercoagulable state of blood caused by hyperuricemia, reduce the risk of related complications such as urate crystal deposition, and play a multi-faceted positive role in the treatment of hyperuricemia.

[0022] Apricot kernel, also known as bitter almond, tastes bitter and is slightly warm in nature. It enters the lung and large intestine meridians. It has the effects of lowering qi, relieving cough and asthma, and moistening the intestines and relieving constipation. It is mainly used for cough and asthma, chest fullness and phlegm, dry intestines and constipation. Modern pharmacological studies have shown that it has the potential to protect the respiratory system, resist oxidation, fight tumors, and regulate metabolism. Studies have found that the unsaturated fatty acids in almonds can regulate the body's water metabolism, thereby improving sugar metabolism and uric acid excretion.

[0023] Rhubarb, also known as General, Huangliang, etc., is bitter in taste and cold in nature. It enters the spleen, stomach, large intestine, liver, and pericardium meridians. It has the effects of purging and attacking accumulation, clearing away heat and purging fire, cooling blood and detoxifying, removing blood stasis and promoting menstruation, and removing dampness and jaundice. It is mainly used for constipation caused by excess heat accumulation, vomiting and bleeding due to blood heat, red eyes and swollen throat, carbuncle, abdominal pain due to intestinal carbuncle, amenorrhea caused by blood stasis, postpartum stasis, traumatic injury, damp-heat dysentery, jaundice and red urine, etc. Modern pharmacological studies have shown that it has the effects of promoting intestinal peristalsis, anti-inflammatory, and regulating blood lipids. Studies have found that rhubarb can promote intestinal peristalsis, accelerate the excretion of metabolic waste in the intestine, including uric acid and its precursors, and reduce the reabsorption of uric acid by the intestine. In addition, its anti-inflammatory and blood lipid regulating effects also help to improve the metabolic disorders and inflammatory states associated with hyperuricemia, and have a positive auxiliary effect on controlling blood uric acid levels.

[0024] According to traditional Chinese medicine, external dampness invasion, or diet that damages the spleen, disordered transportation and transformation, and unfavorable qi transformation will gradually lead to internal dampness and turbidity, which is the cornerstone of the disease. As stated in "Suwen·Bi Lun", "eating too much will damage the stomach and intestines", modern people often overeat fatty and sweet foods and drink a lot of alcohol, which exceeds the spleen and stomach's transportation and transformation capacity, resulting in the inability to transport the essence of water and grain normally, gathering dampness and turning into heat, and dampness and heat accumulating in the body, and easily stagnate in the lower part of the body, affecting water metabolism and hindering uric acid excretion. As mentioned in "Danxi Xinfa·Gout", the heat generated by diet and external cold and dampness are combined to gradually form an internal state of dampness and heat. In terms of emotional disorders, "Lingshu·Ben Shen" says "those who are worried and anxious have blocked qi and cannot move", and long-term emotional discomfort causes liver qi to stagnate and turn into fire, which in turn attacks the spleen and affects the transportation and transformation of the spleen and stomach, and abnormal water and dampness metabolism. Dampness and liver stagnation and fire are combined, and circulate throughout the body with qi and blood, disrupting metabolism in the body, causing imbalance in uric acid production and excretion. When dampness and heat are contracted externally, the dampness and heat from the outside world easily invade the human body during the long summer and other seasons. If the positive energy is insufficient, the internal and external forces will attract each other, blocking the spleen and stomach, blocking the qi mechanism, affecting the gasification of the triple burner, making it difficult to excrete metabolic products such as uric acid, and aggravating the internal dampness and heat. Long-term dampness and heat will block qi and blood. Just as the "Blood Syndrome Theory·Blood Stasis" says "Qi stagnation leads to blood coagulation", its stickiness and heavy turbidity make the qi and blood flow unsmooth, and the blood flow is blocked to form blood stasis. Blood stasis affects the flow of qi and blood, and fights with dampness and heat, causing urate crystals to easily deposit, aggravating the damage to the body. Long-term illness enters the collaterals. According to Ye Tianshi's theory that "the initial disease is in the meridians, and the long-term disease enters the collaterals", hyperuricemia has a long course of disease, evil qi enters the collaterals, the collaterals are stagnant, the flow of qi, blood and body fluids is blocked, and the accumulation of dampness and heat and the endogenous blood stasis are aggravated, forming a vicious cycle. Chinese medicine treats it by clearing away heat, removing dampness and removing blood stasis to achieve good results.

[0025] In the traditional Chinese medicine compound for clearing heat, removing dampness and eliminating blood stasis for treating hyperuricemia, Centella asiatica and Salvia miltiorrhiza are both the main drugs. Centella asiatica can eliminate dampness and reduce jaundice, promote diuresis and relieve stranguria, detoxify and reduce swelling, and expel damp-heat evil from urine; Salvia miltiorrhiza can clear heat, activate blood circulation and remove blood stasis, and can eliminate blood stasis blockage. The two drugs work synergistically to take into account both the symptoms and the root causes, and remove dampness and remove blood stasis in parallel. The minister drugs work synergistically. Astragalus can replenish qi and help transportation and transformation, enhance the body's metabolic function, and can not only assist Centella asiatica in removing dampness, but also assist Salvia miltiorrhiza in promoting blood circulation and removing blood stasis. Its diuretic function is also conducive to uric acid excretion; Coix seeds assist Centella asiatica in clearing heat and removing dampness, while strengthening the spleen to eliminate the source of dampness, and helping dampness evil to be excreted from urine to promote uric acid excretion; Magnolia officinalis can dry dampness and promote qi, assist the main and minister drugs in removing dampness, regulate the qi mechanism so that qi can move and remove dampness, and can also prevent the coldness of the various drugs from damaging the spleen and stomach yang qi too much; Plantago can assist Centella asiatica in clearing heat and promoting diuresis, enhance the excretion of uric acid, and also help to clear damp-heat evil. The adjuvant Atractylodes macrocephala is combined with Coix seed and Astragalus membranaceus to enhance the spleen-strengthening effect, fundamentally improve the spleen and stomach transportation and transformation functions, and prevent the endogenous generation of dampness; Acorus calamus can eliminate dampness and invigorate the spleen, assist the spleen and stomach transportation and transformation, and use its aromatic and moving nature to clear the meridians to relieve long-term disease and stagnation; Atractylodes lancea has a strong ability to dry dampness, and when used with Magnolia bark, Atractylodes macrocephala, etc., it can enhance the function of drying dampness and invigorating the spleen, and remove dampness from the body; Phellodendron chinense clears heat and dries dampness, and when used together with Centella asiatica, Plantago, etc., it can enhance the ability to clear heat and avoid the excessive warming and drying of the medicines; Leonurus japonicus can not only help Salvia miltiorrhiza to promote blood circulation and remove blood stasis, but also can promote diuresis and reduce swelling, and help expel dampness and uric acid from the body. Apricot kernel and rhubarb are both guiding drugs. The former can descend lung qi, because the lung regulates the water channel. If the lung qi is unobstructed, the water channel will be unobstructed, which helps to expel dampness and heat from the urine and prevents the drugs from going too far downward. Rhubarb can clear the bowels and expel heat, so that dampness and heat can be expelled from the stool, enhancing the power of the whole prescription to expel turbidity and toxins, while helping salvia miltiorrhiza to promote blood circulation and remove blood stasis. The above drugs are used together to achieve the effects of clearing heat and dampness, promoting blood circulation and removing blood stasis, and expelling turbidity and toxins. The overall compatibility takes into account both strengthening the foundation and removing evil, so that the body fluids can be distributed smoothly, thus relieving arthralgia.

[0026] Therefore, the present invention combines these drugs so that the efficacy of each drug produces a synergistic effect, can effectively treat hyperuricemia, and has little side effects. The Chinese medicine compound has the functions of clearing away heat and dampness, promoting blood circulation and removing blood stasis, and has a short course of treatment for hyperuricemia, quick effect, and is not easy to relapse after being cured, and is low in price and easy to use.

[0027] As a preferred technical solution of a traditional Chinese medicine compound for clearing away heat, removing dampness and eliminating blood stasis for treating hyperuricemia of the present invention, its effective ingredients are prepared from the following traditional Chinese medicine raw materials in parts by weight:

[0028]

[0029] The present invention also proposes a preparation method of the heat-clearing, dampness-dispelling and blood-stasis-removing Chinese medicinal compound, which comprises the following steps:

[0030] 1) Wash 13 herbs, mix them according to the proportion, add 6 to 8 times the weight of 70% ethanol each time, reflux extract twice, each time for 1 hour, filter and combine the extracts, and concentrate to obtain a fluid extract with a specific gravity of 1.0 to 1.2 at 60°C;

[0031] 2) The fluid extract is made into gel, gel paste, ointment, capsule, granule, pill, oral liquid, pill, syrup, dispersible tablet or effervescent tablet by conventional preparation process. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 , Figure 2 , Figure 3 Comparison of uric acid, creatinine and urea nitrogen levels in rats of each group.

[0033] Figure 4 , Figure 5 Comparison of ADA and XOD levels in each group respectively.

[0034] Figure 6 , Figure 7 These are the comparisons of URAT1 and GLUT9 contents in kidney tissues of rats in each group. DETAILED DESCRIPTION

[0035] The following further describes in detail a Chinese medicinal compound for clearing away heat, removing dampness and eliminating blood stasis for treating hyperuricemia and a preparation method thereof in conjunction with the accompanying drawings and examples.

[0036] Example 1

[0037] (1) Preparation of drugs used in the positive control group and the drug-treated group

[0038] Wash 20g of Lysimachia chinensis, 20g of Salvia miltiorrhiza, 15g of Astragalus, 15g of Coix seeds, 10g of Magnolia officinalis, 10g of Plantago, 9g of Atractylodes macrocephala, 9g of Acorus calamus, 9g of Atractylodes lancea, 10g of Phellodendron chinense, 10g of Leonurus japonicus, 6g of Apricot kernel and 6g of Rhubarb, mix them, add 7 times the weight of 70% ethanol each time, reflux twice, each time for 1 hour, filter and combine the two extracts, concentrate to obtain a fluid extract with a specific gravity of 1.15 at 60°C, and then prepare granules by conventional preparation process.

[0039] The positive control group used allopurinol at a specification of 100 mg / tablet, which was finely ground in a mortar and pestle and prepared into a suspension with a concentration of 1 mg / mL using 3 g / L sodium carboxymethyl cellulose (CMC-Na).

[0040] (2) Establishment of hyperuricemia animal model

[0041] SPF-grade SD rats were used, half male and half female, weighing 180-200g, 8-10 weeks old, and kept in a clean animal laboratory with good ventilation, alternating light, and a temperature of 22±2℃. The water and food were changed every day to adapt to the requirements of this experiment. 24 rats were randomly divided into 3 groups: model group, positive control group, and drug group, with 8 rats in each group; 1.5g·kg -1 and 0.1 g kg -1 Potassium oxonate and adenine solution were gavaged once a day for 28 consecutive days, and the increase in blood uric acid level was regarded as the standard for successful modeling.

[0042] (3) Experimental grouping and drug administration

[0043] The mice with successful modeling were randomly divided into 3 groups, 8 in each group: model group, positive control group, and drug administration group. Another 8 SD rats of half male and half female were randomly selected as the normal control group. After 7 days of adaptive feeding, the doses of the positive control group and the drug administration group were converted according to the principle of equal dosage per unit surface area among various experimental animals. Among them, the positive control group was gavaged with 10 mL / kg volume each time, once a day; the drug administration group was gavaged with a dose of 17.9 g / kg / d. The mice in the normal control group and the model group were gavaged with an equal volume of normal saline. Each group was continuously administered for 8 weeks.

[0044] (4) Index detection

[0045] 1) General morphological observation

[0046] The mental state, activity, diet, coat color changes, etc. of each group of mice were observed; the body weight of the mice was recorded weekly; kidney and liver tissues were obtained after dissection, the weight was measured, and the organ index was calculated.

[0047] During the experimental period, the mice in the blank group were in good mental state, with no abnormal diet and excretion and quick reactions; the mice in the model group were slightly abnormal in mental state compared with normal mice, with reduced activity, reduced food intake and increased water intake. The mice in the other drug-treated groups had improved mental state and more active activities compared with the model group, and no significant changes in food intake and water intake.

[0048] 2) Serum biochemical index determination

[0049] The body weight of all mice was measured 12 hours before the last administration. One hour after the last administration, the mice were killed by dislocating the neck after blood was taken from the eyeballs. The blood was collected in sterile EP tubes and centrifuged at 4°C and 1 006g for 20 minutes. The serum was divided into centrifuge tubes and stored at -80°C. The liver and kidney of the mice were weighed and measured. A portion was placed in 4% paraformaldehyde for HE staining and immunohistochemical staining, and the remaining portion was immersed in liquid nitrogen and quickly frozen and stored at -80°C for later use. The content of uric acid (UA), creatinine (CRE), and blood urea nitrogen (BUN) in the serum of the mice, as well as the activities of xanthine oxidase (XOD) and adenosine deaminase (ADA) were determined according to the instructions of the kit.

[0050] 3) Tissue staining and biochemical index determination

[0051] Kidney tissue was obtained and mixed with pre-cooled physiological saline at a ratio of 1:9 (g:mL), homogenized in a homogenizer, centrifuged at 4°C and 1006g for 10 min, and the supernatant was obtained. The contents of URAT1 and GLUT9 in kidney tissue were determined according to the instructions of the kit.

[0052] The experimental results showed that compared with the normal control group, the serum UA, CRE, BUN, XOD, and ADA levels of the rats in the model group were increased (P<0.05) ( Figure 1-5 The contents of URAT1 and GLUT9 in kidney tissue were increased (P<0.05) ( Figure 6-7 Compared with the model group, UA, CRE, BUN, XOD, and ADA in the positive control group and the drug-treated group were significantly reduced (P<0.05) ( Figure 1-5 The contents of URAT1 and GLUT9 in kidney tissues were also significantly decreased (P<0.05) ( Figure 6-7 shown).

[0053] Long-term high uric acid levels will also cause urate to deposit in the kidneys, leading to uric acid-induced renal damage, which is often judged by renal function-related indicators. The renal coefficient reflects the state of the kidneys. When it increases, it indicates that the kidneys may have pathological changes such as swelling, hypertrophy, congestion, edema, and hyperplasia. SCr is the final product of creatine metabolism and is excreted by the renal tubules; BUN is a nitrogen-containing compound in the blood other than protein, which is filtered out of the body by the glomerulus. Both are important indicators for clinical detection of renal function, and their increased levels indicate renal damage. The metabolism of uric acid in the kidneys mainly involves four processes: filtration, reabsorption, secretion, and reabsorption after secretion. Except for filtration, the remaining processes are all involved in urate transporters. URAT1 is a uric acid anion exchanger that can exchange anions in renal tubular epithelial cells with uric acid in urine, allowing uric acid to return to the blood from the glomerular filtrate. If its expression is upregulated, uric acid reabsorption will be enhanced, causing an increase in SUA levels. Therefore, URAT1 is an important target for drugs that promote uric acid excretion. GLUT9 is both a glucose transporter and a uric acid transporter. It can accelerate the reabsorption of uric acid by transporting glucose. It is another target for the treatment of HUA discovered in recent years.

[0054] The experimental research results of the present invention show that the proposed Chinese herbal compound for clearing away heat, removing dampness and removing blood stasis can reduce the body's UA, CR, BUN, XOD, ADA levels and the URAT1 and GLUT9 contents in kidney tissue (P<0.05), thereby achieving a better therapeutic effect on hyperuricemia.

[0055] The above contents are merely examples and explanations of the concept of the present invention. The technicians in this technical field may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the concept of the invention or exceed the scope defined by the claims, they should all fall within the protection scope of the present invention.

Claims

1. A Chinese medicinal compound for clearing away heat, removing dampness and eliminating blood stasis for treating hyperuricemia, characterized in that: The effective ingredients of the Chinese medicine compound are prepared from the following Chinese medicine raw materials in parts by weight:

2. The Chinese medicinal compound for clearing away heat, removing dampness and eliminating blood stasis as claimed in claim 1, characterized in that: The effective ingredients of the Chinese medicine compound are prepared from the following Chinese medicine raw materials in parts by weight:

3. A method for preparing the heat-clearing, dampness-dispelling and blood stasis-removing Chinese medicinal compound as claimed in claim 1 or 2, characterized in that: Here are the steps: 1) Wash 13 herbs, mix them according to the proportion, add 6 to 8 times the weight of 70% ethanol each time, reflux extract twice, each time for 1 hour, filter and combine the extracts, and concentrate to obtain a fluid extract with a specific gravity of 1.0 to 1.2 at 60°C; 2) The fluid extract is made into gel, gel paste, ointment, capsule, granule, pill, oral liquid, pill, syrup, dispersible tablet or effervescent tablet by conventional preparation process.

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