Method for synergistically repairing heavy metal pollution of wetland by utilizing biology and application of method

Abstract

The invention relates to a method for synergistically remediating wetland heavy metal pollution by biology and application thereof, and the method comprises the following steps: inoculating arbuscular mycorrhizal fungi inoculant and reed into a heavy metal polluted wetland for soil remediation. The method creatively utilizes a symbiotic system of the reed and the arbuscular mycorrhizal fungi to repair the heavy metal cadmium pollution of the wetland sediment. The arbuscular mycorrhizal fungus inoculant is inoculated, so that the biomass of the reed under heavy metal stress is remarkably improved, the content of heavy metal in the reed is improved, pollution of heavy metal cadmium in wetland sediments is reduced, meanwhile, the heavy metal in the wetland sediments is solidified, and the heavy metal cadmium pollution of the wetland can be repaired. The method for repairing the heavy metal pollution of the wetland is easy to operate, the raw materials are easy to obtain, the cost is low, secondary pollution is avoided, good ecological benefits are achieved, an effective means is provided for heavy metal pollution treatment of an aquatic ecosystem, and sustainable development of the ecological environment is promoted.

Description

Technical Field

[0001] The present invention belongs to the technical field of ecological environment restoration, and specifically relates to a method for restoring wetland heavy metal pollution by utilizing biological synergy and its application. Background Art

[0002] In recent years, with the rapid development of industry and agriculture, the problem of heavy metal cadmium pollution in wetland sediments has attracted increasing attention and has become one of the focuses of environmental science research. Microorganisms are one of the most active parts in wetland ecosystems and play an irreplaceable and important role in material circulation. Many microorganisms have the ability to degrade or transform pollutants, and microorganisms have strong adaptability and easy mutation to the environment. They can respond to changes in the environment more promptly. A large number of studies have shown that microorganisms have an important influence on the content of heavy metals in water bodies and sediments. In previous studies on microbial enrichment and transformation of heavy metals, most of them focused on the study of bacterial remediation of heavy metals, while ignoring fungi as the main participants in geochemical chemical cycles and energy conversion, their functions in maintaining and regulating wetland ecosystems, buffering and purifying wetland pollution, and especially their function of working with plants to repair polluted environments.

[0003] Arbuscular mycorrhizal fungi (AMF) are endomycorrhizal fungi that can mutually coexist with most plants. They can form a mycorrhizal symbiotic system with plants, expand the contact area between plant roots and soil, and allow plants to absorb more water and nutrients to promote plant growth and enhance plant stress resistance. They also enrich heavy metals and reduce heavy metal toxicity through growth dilution, chelation, and mycelium enrichment. For example, a Chinese invention patent (application number: 201810006916.6) reported a method for repairing cadmium-contaminated soil using arbuscular mycorrhizal fungi in combination with alfalfa. This method can reduce the total cadmium concentration in the soil from 5.757 mg / kg to a minimum of 0.28 mg / kg. A Chinese invention patent (application number: 201911363519.5) reported a method for treating cadmium-contaminated soil using intraradical Glomus in combination with Torchwood. The symbiosis between intraroot Glomus and Torchwood can promote Torchwood's resistance to cadmium on the one hand, and on the other hand, it can promote Torchwood's absorption of nutrients and promote its growth. The Chinese invention patent (application number: 202110351395.X) introduces a method for remediating cadmium-contaminated soil by intercropping corn and Wedelia chrysogenum and inoculating arbuscular mycorrhizal fungi. In this method, different varieties of corn and Wedelia chrysogenum are intercropped or monocropped, with or without inoculation of Glomus chrysogenum. The experimental results show that Glomus chrysogenum can reduce the cadmium content of corn seeds and increase the cadmium content in Wedelia chrysogenum.

[0004] At present, the technology of using arbuscular mycorrhizal fungi to repair heavy metal cadmium pollution in terrestrial soil is relatively mature, and has shown certain effects in soil heavy metal remediation, but these methods are often not suitable for aquatic environments. At present, the technology of using arbuscular mycorrhizal fungi to repair heavy metal pollution in aquatic ecosystems is particularly lacking, which limits the application of arbuscular mycorrhizal fungi in aquatic heavy metal pollution. Summary of the invention

[0005] In view of the deficiencies of the prior art, the object of the present invention is to provide a method for synergistically repairing heavy metal pollution in wetlands using organisms and its application.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a method for using biological synergistic restoration of heavy metal pollution in wetlands, the method for restoration of heavy metal pollution in wetlands comprising the following steps:

[0008] Arbuscular mycorrhizal fungi and reeds were inoculated into heavy metal contaminated wetlands for soil remediation.

[0009] The present invention aims at the current situation that the means of repairing heavy metal pollution in aquatic ecosystems are relatively single, and proposes a new repair method - using the symbiotic system of reeds and arbuscular mycorrhizal fungi to repair heavy metal cadmium pollution in wetland sediments. Inoculation of arbuscular mycorrhizal fungi significantly increases the biomass of reeds under heavy metal stress, increases the heavy metal content in the reeds, reduces the pollution of heavy metal cadmium in wetland sediments, and solidifies the heavy metals in the wetland sediments, thereby repairing the heavy metal cadmium pollution in the wetland. The method for repairing heavy metal pollution in wetlands involved in the present invention is simple to operate, has easy-to-obtain raw materials, is low in cost, has no secondary pollution, has good ecological benefits, provides effective means for the treatment of heavy metal pollution in aquatic ecosystems, and promotes the sustainable development of the ecological environment.

[0010] Preferably, the arbuscular mycorrhizal fungi include any one of Glomus constrictus, Glomus heteromorpha or Glomus scoparia, or a combination of at least two thereof.

[0011] Preferably, the Glomus caledonium includes the Glomus caledonium AMG16 strain with a deposit number of CGMCC No.41628.

[0012] Preferably, each gram of the arbuscular mycorrhizal fungal agent contains 80-120 (for example, 80, 83, 86, 88, 90, 93, 96, 98, 100, 103, 106, 108, 110, 113, 116, 118, 120, etc.) arbuscular mycorrhizal fungal spores. Other specific point values ​​within this numerical range can be selected, and they will not be described one by one here.

[0013] Preferably, the usage amount of the arbuscular mycorrhizal fungal agent is 1%-5% of the mass of wetland sediment, for example 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, etc. Other specific point values ​​within this numerical range can be selected, which will not be described here one by one.

[0014] Preferably, the arbuscular mycorrhizal fungal agent is obtained by the following method:

[0015] The arbuscular mycorrhizal fungus spores and the host plant are inoculated into the propagation matrix, propagated and cultured, the aerial part of the host plant is removed, and the culture is dried to obtain the arbuscular mycorrhizal fungus agent.

[0016] Preferably, the inoculation amount of the arbuscular mycorrhizal fungal spores is 37-225 (e.g., 37, 40, 50, 60, 70, 80, 90, 100, 110, 130, 150, 170, 190, 200, 220, 225, etc.) arbuscular mycorrhizal fungal spores per kilogram of propagation substrate. Other specific point values ​​within this numerical range can be selected, and they will not be described one by one here.

[0017] Preferably, the host plant comprises Phragmites australis.

[0018] Preferably, the time for the expansion culture is 25-65 days, for example 25 days, 30 days, 35 days, 40 days, 45 days, 50 days, 55 days, 60 days, 65 days, etc. Other specific point values ​​within this numerical range can be selected, and they will not be described one by one here.

[0019] Preferably, the drying temperature is 20-30°C (for example, 20°C, 21°C, 22°C, 23°C, 24°C, 25°C, 26°C, 27°C, 28°C, 29°C, 30°C, etc.), and the drying time is 5-10 days (for example, 5 days, 6 days, 7 days, 8 days, 9 days, 10 days, etc.). Other specific point values ​​within the above numerical range can be selected, and they will not be described one by one here.

[0020] Preferably, the method for repairing heavy metal pollution in wetlands further comprises inoculating a Bacillus amyloliquefaciens agent into the heavy metal polluted wetlands.

[0021] The method for repairing heavy metal pollution in wetlands comprises the following steps: inoculating arbuscular mycorrhizal fungi agents, bacillus amyloliquefaciens agents and reeds into heavy metal polluted wetlands to carry out soil repair.

[0022] The present invention also creatively discovered that arbuscular mycorrhizal fungi and Bacillus amyloliquefaciens play a synergistic role in repairing heavy metal cadmium pollution in wetlands.

[0023] Preferably, the Bacillus amyloliquefaciens includes the Bacillus amyloliquefaciens A3 strain with a deposit number of CGMCC No.6676.

[0024] Preferably, the viable count of the Bacillus amyloliquefaciens agent is not less than 5 x 10 8 CFU / mL (e.g. 5x10 8 CFU / mL, 6x10 8 CFU / mL, 7x10 8 CFU / mL, 8x10 8 CFU / mL, 9x10 8 CFU / mL, 5x10 9 CFU / mL, 5x10 10 CFU / mL, etc.) or 2x10 10 CFU / g (e.g. 2x10 10 CFU / g, 3x10 10 CFU / g, 4x10 10 CFU / g, 6x10 10 CFU / g, 8x10 10 CFU / g, 2x10 11 CFU / g, 2x10 12 CFU / g, 2x10 13 CFU / g, etc.), other specific point values ​​within the above numerical range can be selected, and they will not be described here one by one.

[0025] Preferably, the usage amount of the Bacillus amyloliquefaciens agent is 1%-3% of the mass of wetland sediment, for example 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 2.2%, 2.4%, 2.6%, 2.8%, 3%, etc. Other specific point values ​​within this numerical range can be selected, which will not be described here one by one.

[0026] In a second aspect, the present invention provides application of the method for repairing heavy metal pollution in wetlands according to the first aspect in wetland restoration.

[0027] Compared with the prior art, the present invention has the following beneficial effects:

[0028] In view of the current situation that the means of repairing heavy metal pollution in aquatic ecosystems are relatively single, the present invention proposes a new repair method - using the symbiotic system of reeds and arbuscular mycorrhizal fungi to repair heavy metal cadmium pollution in wetland sediments. Inoculation of arbuscular mycorrhizal fungi significantly increases the biomass of reeds under heavy metal stress, increases the heavy metal content in the reeds, and reduces the pollution of heavy metal cadmium in wetland sediments. At the same time, it has a solidifying effect on the heavy metals in the wetland sediments, and can repair the heavy metal cadmium pollution in the wetland. The method for repairing heavy metal pollution in wetlands involved in the present invention is simple to operate, the raw materials are easily available, the cost is low, there is no secondary pollution, and there is good ecological benefit. It provides an effective means for the treatment of heavy metal pollution in aquatic ecosystems and promotes the sustainable development of the ecological environment.

[0029] The AMG16 strain involved in the present invention is classified and named Glomus caledonium, and the preservation unit is the General Microbiology Center of China Microbiological Culture Collection Administration. The preservation time is November 15, 2024, the preservation number is CGMCC No.41628, and the address is: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.

[0030] The A3 strain involved in the present invention is classified and named as Bacillus amyloliquefaciens, and the deposit unit is the General Microbiology Center of China Microorganism Culture Collection Administration. The deposit time is October 15, 2012, the deposit number is CGMCC No.6676, and the address is: Datun Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences. DETAILED DESCRIPTION

[0031] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0032] In the following examples, unless otherwise specified, the reagents and consumables used were purchased from conventional reagent manufacturers in the field; unless otherwise specified, the experimental methods and technical means used were conventional methods and means in the field.

[0033] The AMG16 strain involved in the following examples is classified and named Glomus caledonium, the preservation date is November 15, 2024, and the preservation number is CGMCC No.41628.

[0034] The A3 strain involved in the following examples is classified and named as Bacillus amyloliquefaciens, deposited on October 15, 2012, and has a deposit number of CGMCC No.6676.

[0035] Preparation Example 1

[0036] This preparation example provides an arbuscular mycorrhizal fungal agent AMG16.

[0037] Wetland sandy loam soil was obtained from a sampling point of a heavy metal cadmium-contaminated wetland and sterilized at 121°C for 30 minutes as a propagation matrix. Plastic pots were sterilized with 8% sodium hypochlorite, and 3kg of propagation matrix, 300 AMG16 spores and 1L of ultrapure water were added to each pot. Reed seedlings obtained from the sampling point were planted in plastic pots, with 7 plants planted in each pot, and the growth conditions of the reeds in each pot were roughly similar. After 45 days of cultivation, a host plant infected with arbuscular mycorrhizal fungi and a sandy loam matrix containing arbuscular mycorrhizal fungi spores and extraradical hyphae were obtained, the above-ground part of the reed was removed, the culture in the pot was poured out and evenly separated and placed in an incubator at 25°C for 8 days to obtain an arbuscular mycorrhizal fungus agent after propagation, and each gram of the Scottish Glomus AMG16 agent contained 100 Scottish Glomus AMG16 spores.

[0038] Preparation Example 2

[0039] This preparation example provides an arbuscular mycorrhizal fungal agent AMG16.

[0040] Wetland sandy loam soil was obtained from a sampling point of a heavy metal cadmium-contaminated wetland and sterilized at 121°C for 30 minutes as a propagation matrix. Plastic pots were sterilized with 8% sodium hypochlorite, and 3kg of propagation matrix, 250 AMG16 spores and 1L of ultrapure water were added to each pot. Reed seedlings obtained from the sampling point were planted in plastic pots, with 7 plants planted in each pot, and the growth conditions of the reeds in each pot were roughly similar. After 50 days of cultivation, a host plant infected with arbuscular mycorrhizal fungi and a sandy loam matrix containing arbuscular mycorrhizal fungi spores and extraradical hyphae were obtained, the above-ground part of the reed was removed, the culture in the pot was poured out and evenly separated and placed in an incubator at 25°C for 10 days to obtain an arbuscular mycorrhizal fungus agent after propagation, and each gram of the Scottish Glomus AMG16 agent contained 120 Scottish Glomus AMG16 spores.

[0041] Preparation Example 3

[0042] This preparation example provides an arbuscular mycorrhizal fungal agent AMG16.

[0043] Wetland sandy loam soil was obtained from a sampling point of a heavy metal cadmium-contaminated wetland and sterilized at 121°C for 30 minutes as a propagation matrix. Plastic pots were sterilized with 8% sodium hypochlorite, and 3kg of propagation matrix, 200 AMG16 spores and 1L of ultrapure water were added to each pot. Reed seedlings obtained from the sampling point were planted in plastic pots, with 7 plants planted in each pot, and the growth conditions of the reeds in each pot were roughly similar. After 35 days of cultivation, a host plant infected with arbuscular mycorrhizal fungi and a sandy loam matrix containing arbuscular mycorrhizal fungi spores and extraradical hyphae were obtained, the above-ground part of the reed was removed, the culture in the pot was poured out and evenly separated and placed in an incubator at 25°C for 8 days to obtain an arbuscular mycorrhizal fungus agent after propagation, and each gram of the Scottish Glomus AMG16 agent contained 80 Scottish Glomus AMG16 spores.

[0044] Preparation Example 4

[0045] This preparation example provides a Bacillus amyloliquefaciens A3 bacterial agent.

[0046] Take out the Bacillus amyloliquefaciens A3 strain from the storage tube and use LB solid medium for recovery. The culture condition is 37℃ for 48h. Pick a single colony on the plate and inoculate it into 50mL LB medium. Incubate it at 37℃ with shaking for 24h. Then inoculate the seed liquid into 500mL LB medium at a 5% inoculum amount and continue to culture for 24h until the concentration of the bacterial liquid reaches 40x10 8 The prepared liquid strain was inoculated into the fermentation medium (medium composition: peptone 5g, NaCl 0.25g, KCl0.3g, K2HPO4 0.25g, MgSO4·7H2O 0.5g, FeSO4·7H2O 0.01g, ultrapure water 1L, pH=7.2), the fermentation temperature was controlled at 37°C, the fermentation time was 36h, and the viable count was 5x10 8 CFU / mL of Bacillus amyloliquefaciens A3 liquid inoculum.

[0047] Example 1

[0048] This embodiment provides a method for repairing humidity heavy metal pollution, the steps are as follows:

[0049] (1) The arbuscular mycorrhizal fungus AMG16 agent obtained in Preparation Example 1 and the Bacillus amyloliquefaciens A3 agent obtained in Preparation Example 4 were inoculated into wetland sediments with a heavy metal cadmium concentration of 10 mg / kg at a mass ratio of 3%, respectively. After inoculation, the sediments were placed in sterile basins, and 1 L of ultrapure water was added to each basin.

[0050] (2) After surface disinfection, the reed seedlings collected in situ with a height of 20-35 cm and 5-10 leaves were planted in the sterile pots prepared in the above (1). Eight reed seedlings with similar growth conditions were planted in each sterile pot. The light time in the greenhouse was controlled to be 14 h and the dark time was 10 h. The room temperature was 25°C. Ultrapure water was used every three days for irrigation. The repair time was 90 days.

[0051] Example 2

[0052] This embodiment provides a method for repairing humidity heavy metal pollution, the steps are as follows:

[0053] (1) The arbuscular mycorrhizal fungus AMG16 agent obtained in Preparation Example 2 and the Bacillus amyloliquefaciens A3 agent obtained in Preparation Example 4 were inoculated into wetland sediments with a heavy metal cadmium concentration of 10 mg / kg at a mass ratio of 2%, respectively. After inoculation, the sediments were placed in sterile basins, and 1 L of ultrapure water was added to each basin.

[0054] (2) After surface disinfection, the reed seedlings collected in situ with a height of 20-35 cm and 5-10 leaves were planted in the sterile pots prepared in the above (1). Eight reed seedlings with similar growth conditions were planted in each sterile pot. The light time in the greenhouse was controlled to be 14 h and the dark time was 10 h. The room temperature was 25°C. Ultrapure water was used every three days for irrigation. The repair time was 90 days.

[0055] Example 3

[0056] This embodiment provides a method for repairing humidity heavy metal pollution, the steps are as follows:

[0057] (1) The arbuscular mycorrhizal fungus AMG16 agent obtained in Preparation Example 3 and the Bacillus amyloliquefaciens A3 agent obtained in Preparation Example 4 were inoculated into wetland sediments with a heavy metal cadmium concentration of 10 mg / kg at a mass ratio of 4%, respectively. After inoculation, the sediments were placed in sterile basins, and 1 L of ultrapure water was added to each basin.

[0058] (2) After surface disinfection, the reed seedlings collected in situ with a height of 20-35 cm and 5-10 leaves were planted in the sterile pots prepared in the above (1). Eight reed seedlings with similar growth conditions were planted in each sterile pot. The light time in the greenhouse was controlled to be 14 h and the dark time was 10 h. The room temperature was 25°C. Ultrapure water was used every three days for irrigation. The repair time was 90 days.

[0059] Example 4

[0060] This embodiment provides a method for repairing humidity heavy metal pollution, which differs from Example 1 only in that the number of live bacteria of the arbuscular mycorrhizal fungus AMG16 agent obtained in Preparation Example 1 is replaced by Glomus (purchased from Gray Algae Biology, product number HZB413471, NBCI classification number: 1921602), and other conditions remain unchanged.

[0061] Example 5

[0062] This embodiment provides a method for repairing humidity heavy metal pollution, which differs from Example 1 only in that the number of live bacteria of the Bacillus amyloliquefaciens A3 agent obtained in Preparation Example 4 is replaced by Bacillus amyloliquefaciens ATCC23350, and other conditions remain unchanged.

[0063] Example 6

[0064] This embodiment provides a method for repairing humidity heavy metal pollution, the steps are as follows:

[0065] (1) The arbuscular mycorrhizal fungus AMG16 agent obtained in Preparation Example 1 was inoculated into wetland sediments with a heavy metal cadmium concentration of 10 mg / kg at a mass ratio of 3%. After inoculation, the sediments were placed in sterile basins, and 1 L of ultrapure water was added to each basin.

[0066] (2) After surface disinfection, the reed seedlings collected in situ with a height of 20-35 cm and 5-10 leaves were planted in the sterile pots prepared in the above (1). Eight reed seedlings with similar growth conditions were planted in each sterile pot. The light time in the greenhouse was controlled to be 14 h and the dark time was 10 h. The room temperature was 25°C. Ultrapure water was used every three days for irrigation. The repair time was 90 days.

[0067] Example 7

[0068] This embodiment provides a method for repairing heavy metal pollution in humidity, which differs from Embodiment 6 only in that the heavy metal cadmium concentration of the wetland sediment in step (1) is 20 mg / kg, and other conditions remain unchanged.

[0069] Comparative Example 1

[0070] This comparative example provides a method for repairing humidity heavy metal pollution, the steps are as follows:

[0071] (1) The Bacillus amyloliquefaciens A3 inoculum obtained in Preparation Example 4 was inoculated into wetland sediments with a heavy metal cadmium concentration of 10 mg / kg at a mass ratio of 3%. After inoculation, the sediments were placed in sterile basins, and 1 L of ultrapure water was added to each basin.

[0072] (2) After surface disinfection, the reed seedlings collected in situ with a height of 20-35 cm and 5-10 leaves were planted in the sterile pots prepared in the above (1). Eight reed seedlings with similar growth conditions were planted in each sterile pot. The light time in the greenhouse was controlled to be 14 h and the dark time was 10 h. The room temperature was 25°C. Ultrapure water was used every three days for irrigation. The repair time was 90 days.

[0073] Comparative Example 2

[0074] This comparative example provides a method for repairing heavy metal pollution in humidity, which differs from Example 1 only in that no bacterial agent is inoculated, and reed seedlings with a height of 20-35 cm and 5-10 leaves collected in situ are surface disinfected and planted in wetland sediments with a heavy metal cadmium concentration of 10 mg / kg. Eight reed seedlings with similar growth conditions are planted in each sterile pot, the light time in the greenhouse is controlled to be 14 h and the dark time to be 10 h, the room temperature is 25°C, and ultrapure water is poured every three days. The repair time is 90 days.

[0075] Comparative Example 3

[0076] This comparative example provides a method for repairing heavy metal pollution in humidity, which differs from comparative example 2 only in that the heavy metal cadmium concentration in the wetland sediment is 20 mg / kg, and other conditions remain unchanged.

[0077] Test Case

[0078] This test example tests the repair results of Examples 1-7 and Comparative Examples 1-3.

[0079] Control group 1: The only difference from control group 2 is that the heavy metal cadmium concentration in the wetland sediment is 0 mg / kg, and other conditions remain unchanged.

[0080] Control group 2: The only difference from Example 6 is that the heavy metal cadmium concentration in the wetland sediment is 0 mg / kg, and other conditions remain unchanged.

[0081] The mycorrhizal infection rate of wheat roots was determined by ink vinegar staining method.

[0082] The nitrogen, phosphorus and potassium contents in the roots and leaves of reeds were oxidized and digested according to the "Soil Agricultural Chemical Analysis Methods", in which nitrogen and phosphorus were oxidized into ammonium salts and phosphates. The nitrogen content was measured by the alkaline diffusion method, and the phosphorus was measured by the molybdenum antimony colorimetric method.

[0083] Microwave digestion method was used to determine the Cd content in sediments. 0.25 g soil sample was weighed and placed in a digestion tank. 1 mL of deionized water was used to moisten the sample. 6 mL of HNO3, 3 mL of HCl, and 2 mL of HF were added in sequence. The digestion procedure was set according to Table 1. After digestion, the sample was acidified at 140 °C until the liquid became viscous. The sample was transferred to a 25 mL volumetric flask and the Cd content in the sample solution was determined by flame method. The results are shown in Table 2.

[0084] Table 1

[0085] step Digestion temperature Keep time Pressure limit 1 120℃ 3min 20bar 2 160℃ 3 min 35bar 3 190℃ 25 min 40bar

[0086] Table 2

[0087]

[0088]

[0089] The results in Table 2 show that the arbuscular mycorrhizal fungi AMG16 has an infection effect on reeds, and the infection rate can reach 57.2% without the application of cadmium. Although heavy metals in the soil will reduce the infection rate of arbuscular mycorrhizal fungi on reeds, the infection rate of the fungi on reeds can still reach 50.7% and 54.1% after applying 10mg / kg and 20mg / kg cadmium stress. The symbiotic system of reeds and arbuscular mycorrhizal fungi can significantly increase the removal of cadmium in wetland sediments. After inoculation with AMG16, the cadmium removal rates in the soil under 10mg / kg and 20mg / kg cadmium stress increased by 68% and 13.5%, respectively, compared with the uninoculated agents.

[0090] Table 2

[0091]

[0092] It can be seen from the data in Table 2 that the method for using biological synergistic restoration of heavy metal pollution in wetlands provided in the present application can effectively increase the heavy metal content in reeds and reduce the pollution of heavy metal cadmium in wetland sediments.

[0093] By comparing Example 1 with Example 4, it can be seen that the specific arbuscular mycorrhizal fungal agent AMG16 used in the present invention can effectively repair humidity heavy metal pollution compared with other arbuscular mycorrhizal fungal agents.

[0094] By comparing Example 1 with Example 5, it can be seen that the specific Bacillus amyloliquefaciens A3 used in the present invention can effectively repair humidity heavy metal pollution compared with other Bacillus amyloliquefaciens.

[0095] By comparing Example 1 with Example 6 and Comparative Example 1, it can be seen that the arbuscular mycorrhizal fungus AMG16 and Bacillus amyloliquefaciens A3 achieve a synergistic effect in reducing the total cadmium removal rate in wetland sediments.

[0096] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0097] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0098] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A method for using biological synergistic restoration of heavy metal pollution in wetlands, characterized in that: The method for repairing heavy metal pollution in wetlands comprises the following steps: Arbuscular mycorrhizal fungi and reeds were inoculated into heavy metal contaminated wetlands for soil remediation.

2. The method for repairing heavy metal pollution in wetlands according to claim 1, characterized in that: The arbuscular mycorrhizal fungi include any one of Glomus constrictus, Glomus heteromorphus or Glomus scoparius, or a combination of at least two thereof; Preferably, the Glomus caledonium includes the Glomus caledonium AMG16 strain with a deposit number of CGMCC No.41628.

3. The method for repairing heavy metal pollution in wetlands according to claim 1 or 2, characterized in that: Each gram of the arbuscular mycorrhizal fungus agent contains 80-120 arbuscular mycorrhizal fungus spores.

4. The method for repairing heavy metal pollution in wetlands according to any one of claims 1 to 3, characterized in that: The usage amount of the arbuscular mycorrhizal fungi agent is 1%-5% of the wetland sediment mass.

5. The method for repairing heavy metal pollution in wetlands according to any one of claims 1 to 4, characterized in that: The arbuscular mycorrhizal fungal agent is obtained by the following method: The arbuscular mycorrhizal fungus spores and the host plant are inoculated into the propagation matrix, propagated and cultured, the aerial part of the host plant is removed, and the culture is dried to obtain the arbuscular mycorrhizal fungus agent.

6. The method for repairing heavy metal pollution in wetlands according to any one of claims 1 to 5, characterized in that: The method for repairing heavy metal pollution in wetlands also includes inoculating a Bacillus amyloliquefaciens inoculant into the heavy metal polluted wetlands.

7. The method for repairing heavy metal pollution in wetlands according to claim 6, characterized in that: The Bacillus amyloliquefaciens includes the Bacillus amyloliquefaciens A3 strain with a deposit number of CGMCC No.6676.

8. The method for repairing heavy metal pollution in wetlands according to claim 6 or 7, characterized in that: The viable bacterial count of the Bacillus amyloliquefaciens agent is not less than 5x10 8 CFU / mL or 2x10 10 CFU / g.

9. The method for repairing heavy metal pollution in wetlands according to any one of claims 6 to 8, characterized in that: The usage amount of the Bacillus amyloliquefaciens agent is 1%-3% of the wetland sediment mass.

10. Use of the method for repairing heavy metal pollution in wetlands according to any one of claims 1 to 9 in wetland restoration.

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