Soil biological organic conditioner for treating acid and alkali and preparation method thereof

Abstract

The invention relates to a biological organic conditioner for treating acid and alkali soil and a preparation method of the biological organic conditioner, and belongs to the technical field of soil conditioners. The soil biological organic conditioner for treating acid and alkali is characterized by being prepared from the following components in parts by weight: 10-15 parts of compound microorganisms, 10-15 parts of a modified water-retaining agent, 15-30 parts of a functional additive and 30-40 parts of decomposed animal waste. The soil conditioner can significantly improve the soil structure, adjust the soil pH value, relieve the acidification and salinization degree according to the actual conditions of the soil, restore the soil microbial ecological environment and improve the growth and development indexes of crops.

Description

Technical Field

[0001] The invention belongs to the technical field of soil conditioners and relates to a biological organic conditioner for treating acid and alkaline soil and a preparation method thereof. Background Art

[0002] Soil is one of the basic conditions necessary for plant growth. Soil can provide plants with rich nutrients and water, thereby promoting plant growth and development. Soil contains a large amount of organic matter, inorganic ions and bacteria, etc. It is a traditional Chinese medicine medium for photosynthesis and gas exchange, and plays an important role in the conversion of photosynthetic energy. In addition, soil also contains many important substances and elements, such as nitrogen, phosphorus, potassium, etc., which play an irreplaceable role in plant reproduction.

[0003] As we all know, only good soil can grow good crops. The pH value of the soil will affect the normal growth of crops, and then affect the yield of crops. Many crops like to live in neutral soil. Soil that is too acidic or too alkaline is not suitable for crop growth.

[0004] The pH value of the soil also affects the fertility of the soil. Inappropriate pH value of the soil will reduce the effective components of the soil. For example, the solubility of calcium and magnesium in highly alkaline soil will decrease, and the effectiveness will also decrease. Too acidic or alkaline soil will inhibit the activity of soil microorganisms. Summary of the invention

[0005] The main purpose of the present invention is to provide a soil biological organic conditioner, which is suitable for acidified or saline-alkali soils, can adaptably improve soil conditions according to actual soil conditions, adjust pH, improve overall soil properties, and increase crop performance and yield.

[0006] The present invention adopts the following technical solutions to achieve the above purpose:

[0007] A soil biological organic conditioner for treating acid and alkali, mainly prepared from the following components:

[0008] 10-15 parts by weight of composite microorganisms, 10-15 parts by weight of modified water-retaining agent, 15-30 parts by weight of functional additives, and 30-40 parts by weight of decomposed animal excrement.

[0009] The present invention provides a method for preparing the above-mentioned conditioning agent, comprising the following steps:

[0010] Step 1, raw material preparation: separating the yellow outer peel and the inner peel sponge layer of the grapefruit peel; drying and crushing the inner peel sponge layer to obtain the grapefruit inner peel powder for preparing the modified water-retaining agent; drying the yellow outer peel, distilling to remove volatile components, and crushing the grapefruit outer peel powder for preparing the composite microorganism;

[0011] Step 2, preparing the composite microorganism: inoculating the seed liquid of Streptomyces hygroscopicus with acanthosporium into a fermentation medium prepared by grapefruit exocarp powder, corn flour, glucose, potassium dihydrogen phosphate, and calcium carbonate, and culturing at 25-30° C. for 20-24 hours to obtain a fermentation liquid containing Streptomyces hygroscopicus with acanthosporium; adding Bacillus subtilis, Bacillus megaterium, and Candida utilis to the fermentation liquid, and mixing well to obtain the composite microorganism;

[0012] Step 3, preparing a modified water-retaining agent: soaking grapefruit endocarp powder in a hydrogen peroxide aqueous solution for 1.5-2 hours, adding behenamidopropyl dimethylamine and acrylic acid and mixing to obtain a mixed solution; adding potassium hydroxide aqueous solution to the mixed solution to adjust the pH of the mixed solution to 7.5-8.0, adding N,N-methylenebisacrylamide and potassium persulfate, stirring evenly, and reacting at 60-65° C., adding a mixed solution of sodium alginate and polyvinyl alcohol after the reaction is completed, stirring at 90-100° C. until viscous, drying, and crushing to obtain a modified water-retaining agent;

[0013] Step 4, mixing the obtained composite microorganisms, modified water-retaining agent, functional additives and decomposed animal feces, drying and crushing them to obtain a soil biological organic conditioner.

[0014] Furthermore, in the preparation of the composite microorganism in step 2, each 100 mL of fermentation medium contains 1-1.5 g of grapefruit peel powder, 2-3 g of corn flour, 1-1.5 g of glucose, 0.01-0.05 g of potassium dihydrogen phosphate, and 0.2-0.3 g of calcium carbonate. It is prepared with deionized water, the pH is adjusted to 7.0, and it is used after sterilization.

[0015] Furthermore, in the step 2 of preparing the composite microorganism, the inoculation amount of the seed liquid of Streptomyces hygroscopicus is 1-1.5% of the volume of the fermentation medium.

[0016] Furthermore, in the preparation of the composite microorganism in step 2, the number of effective live bacteria in the seed solution of Streptomyces hygroscopicus is 3 billion / mL.

[0017] Furthermore, in the preparation of the composite microorganism in step 2, the effective viable count of Streptomyces hygroscopicus in the obtained fermentation broth is 2-4 billion / mL.

[0018] Furthermore, in the preparation of the composite microorganism in step 2, the amount of Bacillus subtilis added is 0.2-0.3 g / mL, the amount of Bacillus megaterium added is 0.1-0.15 g / mL, and the amount of Candida utilis added is 0.03-0.05 g / mL.

[0019] Furthermore, in the preparation of the composite microorganism in step 2, the effective viable count of Bacillus subtilis is 20 billion / g, the effective viable count of Bacillus megaterium is 10 billion / g, and the effective viable count of Candida utilis is 20 billion / g.

[0020] Furthermore, in the preparation of the modified water-retaining agent in step 3, the usage amounts of the components are: 7-10 parts by weight of grapefruit endocarp powder, 1-1.5 parts by weight of behenamidopropyl dimethylamine, 25-30 parts by weight of acrylic acid, 0.15-0.25 parts by weight of N,N-methylenebisacrylamide, and 0.1-0.15 parts by weight of potassium persulfate.

[0021] Furthermore, in the preparation of the modified water-retaining agent in step 3, the aqueous hydrogen peroxide solution is a hydrogen peroxide solution with a mass fraction of 15%.

[0022] Furthermore, in the preparation of the modified water-retaining agent in step 3, the potassium hydroxide aqueous solution is a potassium hydroxide aqueous solution with a mass fraction of 25%-30%.

[0023] Furthermore, in the preparation of the modified water-retaining agent in step 3, the amount of the mixed solution of sodium alginate and polyvinyl alcohol used is 30-40 parts by weight.

[0024] Furthermore, in the preparation of the modified water-retaining agent in step 3, the mass fraction of sodium alginate in the mixed solution of sodium alginate and polyvinyl alcohol is 8%-10%, and the mass fraction of polyvinyl alcohol is 3%-5%.

[0025] Furthermore, the functional additive contains 2-7 parts by weight of zeolite powder, 3-8 parts by weight of oyster shell powder, and 10-15 parts by weight of potassium humate.

[0026] The present invention has the following beneficial effects:

[0027] 1. In the present invention, the best microbial species and their ratios are obtained by screening microbial species. The composite microorganisms in the present invention can adjust the acidity and alkalinity of the soil according to the actual environment, and have a good ability to repair the soil; at the same time, the microorganisms can also generate polysaccharide components through their own metabolic activities, combine with soil aggregates, improve soil structure, enhance soil fertility, promote and regulate plant growth, and enhance plant survival ability.

[0028] 2. The modified water-retaining agent prepared in the present invention has a high water storage and moisture retention performance. When it is applied to the soil, it can improve the soil structure, change the soil from dense to loose, increase the porosity, improve the soil permeability and air permeability, improve the soil matrix environment, be beneficial to microbial activity, and promote the growth and development of crops.

[0029] 3. Among the functional additives used in the present invention, potassium humate can improve the soil aggregate structure, loosen the soil, improve the soil's ability to retain water and fertilizer, and adjust the pH value. At the same time, it can also improve the physical and chemical properties of the soil, improve the soil's ability to retain and absorb fertilizers, and improve the soil's buffering capacity; zeolite has the dual regulation of acid and alkali, and can increase the soil pH value on acidic soils and reduce the soil pH value on alkaline soils, playing a buffering role. Zeolite can adsorb cations in soil colloids, thereby promoting the coagulation of soil particles, accelerating the formation of soil aggregates, reducing soil bulk density, increasing soil porosity, making the soil loose, improving soil permeability, and improving drainage and infiltration capacity; it can also reduce soil salinity and alkalinity; it can improve the soil's ability to retain ammonium ions, phosphorus ions and potassium ions, and improve nutrient utilization. DETAILED DESCRIPTION

[0030] The present invention is further explained below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not used to limit the scope of the present invention. After reading the present invention, modifications of various equivalent forms of the present invention by those skilled in the art all fall within the scope protected by the claims of this application. The seed liquid of Streptomyces hygroscopicus with a spiny spore used in the following embodiments is a strain of Streptomyces hygroscopicus with a strain collection number of CCTCC AA 87008 purchased from the China Center for Type Culture Collection, which is obtained after activation and expansion culture in our laboratory; other microorganisms such as Bacillus subtilis are existing commercially available products and can be purchased.

[0031] Example 1

[0032] Step 1, raw material preparation: separating the yellow outer peel and the inner peel sponge layer of the grapefruit peel; drying and crushing the inner peel sponge layer to obtain the grapefruit inner peel powder for preparing the modified water-retaining agent; drying the yellow outer peel, distilling to remove volatile components, and crushing the grapefruit outer peel powder for preparing the composite microorganism;

[0033] Step 2, preparing the composite microorganism: weighing each component according to the ratio of 1.5g of grapefruit peel powder, 2g of corn flour, 1.5g of glucose, 0.01g of potassium dihydrogen phosphate, and 0.3g of calcium carbonate per 100mL of fermentation medium, adding deionized water to prepare, adjusting the pH to 7.0, and sterilizing before use; inoculating the seed liquid of Streptomyces hygroscopicus with an effective viable count of 3 billion / mL into the above-mentioned fermentation medium according to an inoculation amount of 1.5% of the volume of the fermentation medium, culturing at 30°C for 20h, and obtaining a fermentation liquid containing about 4 billion / mL of Streptomyces hygroscopicus with an effective viable count; adding 0.3g / mL of Bacillus subtilis with an effective viable count of 20 billion / g, 0.1g / mL of Bacillus megaterium with an effective viable count of 10 billion / g, and 0.05g / mL of Candida utilis with an effective viable count of 20 billion / g to the fermentation liquid, mixing well, and obtaining the composite microorganism;

[0034] Step 3, preparing a modified water-retaining agent: soaking 7 parts by weight of grapefruit endocarp powder in a 15% by weight aqueous solution of hydrogen peroxide for 1.5 hours, adding 1.5 parts by weight of behenamidopropyl dimethylamine and 25 parts by weight of acrylic acid and mixing well to obtain a mixed solution; adding a 30% by weight aqueous solution of potassium hydroxide to the mixed solution to adjust the pH of the mixed solution to 8.0, adding 0.25 parts by weight of N,N-methylenebisacrylamide and 0.1 parts by weight of potassium persulfate, stirring well, and reacting at 65° C., adding 40 parts by weight of a mixed solution of sodium alginate (10% by weight) and polyvinyl alcohol (3% by weight) after the reaction is completed, stirring at 100° C. until a viscous state, drying, and crushing to obtain a modified water-retaining agent;

[0035] Step 4, 15 parts by weight of the obtained composite microorganisms, 10 parts by weight of the modified water-retaining agent, 30 parts by weight of the functional additives (containing 7 parts by weight of zeolite powder, 8 parts by weight of oyster shell powder, and 15 parts by weight of potassium humate), and 30 parts by weight of decomposed animal feces are mixed, dried, and crushed to obtain a soil biological organic conditioner.

[0036] Example 2

[0037] Step 1, raw material preparation: separating the yellow outer peel and the inner peel sponge layer of the grapefruit peel; drying and crushing the inner peel sponge layer to obtain the grapefruit inner peel powder for preparing the modified water-retaining agent; drying the yellow outer peel, distilling to remove volatile components, and crushing the grapefruit outer peel powder for preparing the composite microorganism;

[0038] Step 2, preparing a composite microorganism: weighing each component according to a ratio of 1 g of grapefruit peel powder, 3 g of corn flour, 1 g of glucose, 0.05 g of potassium dihydrogen phosphate, and 0.2 g of calcium carbonate per 100 mL of fermentation medium, adding deionized water to prepare, adjusting the pH to 7.0, and sterilizing before use; inoculating a seed solution of Streptomyces hygroscopicus with an effective viable count of 3 billion / mL into the above-mentioned fermentation medium at an inoculation amount of 1% of the volume of the fermentation medium, culturing at 25° C. for 24 hours to obtain a fermentation liquid containing an effective viable count of about 2 billion / mL of Streptomyces hygroscopicus; adding 0.2 g / mL of Bacillus subtilis with an effective viable count of 20 billion / g, 0.15 g / mL of Bacillus megaterium with an effective viable count of 10 billion / g, and 0.03 g / mL of Candida utilis with an effective viable count of 20 billion / g to the fermentation liquid, mixing well, and obtaining a composite microorganism;

[0039] Step 3, preparing a modified water-retaining agent: soaking 10 parts by weight of grapefruit endocarp powder in a 15% by weight aqueous solution of hydrogen peroxide for 2 hours, adding 1 part by weight of behenamidopropyl dimethylamine and 30 parts by weight of acrylic acid and mixing well to obtain a mixed solution; adding a 25% by weight aqueous solution of potassium hydroxide to the mixed solution to adjust the pH of the mixed solution to 7.5, adding 0.15 parts by weight of N,N-methylenebisacrylamide and 0.15 parts by weight of potassium persulfate, stirring well, and reacting at 60° C., adding 30 parts by weight of a mixed solution of sodium alginate (mass fraction of 8%) and polyvinyl alcohol (mass fraction of 5%) after the reaction is completed, stirring at 90° C. until viscous, drying, and crushing to obtain a modified water-retaining agent;

[0040] Step 4, 10 parts by weight of the obtained composite microorganisms, 15 parts by weight of the modified water-retaining agent, 15 parts by weight of the functional additive (containing 2 parts by weight of zeolite powder, 3 parts by weight of oyster shell powder, and 10 parts by weight of potassium humate), and 40 parts by weight of decomposed animal feces are mixed, dried, and crushed to obtain a soil biological organic conditioner.

[0041] Example 3

[0042] Step 1, raw material preparation: separating the yellow outer peel and the inner peel sponge layer of the grapefruit peel; drying and crushing the inner peel sponge layer to obtain the grapefruit inner peel powder for preparing the modified water-retaining agent; drying the yellow outer peel, distilling to remove volatile components, and crushing the grapefruit outer peel powder for preparing the composite microorganism;

[0043] Step 2, preparing a composite microorganism: weighing each component according to a ratio of 1.3 g of grapefruit peel powder, 2.5 g of corn flour, 1.2 g of glucose, 0.03 g of potassium dihydrogen phosphate, and 0.25 g of calcium carbonate per 100 mL of fermentation medium, adding deionized water to prepare, adjusting the pH to 7.0, and sterilizing before use; inoculating a seed solution of Streptomyces hygroscopicus with an effective viable count of 3 billion / mL into the above-mentioned fermentation medium at an inoculation amount of 1.5% of the volume of the fermentation medium, culturing at 25° C. for 20 hours, and obtaining a fermentation liquid containing about 3.3 billion / mL of Streptomyces hygroscopicus with an effective viable count of about 3.3 billion / mL; adding 0.25 g / mL of Bacillus subtilis with an effective viable count of 20 billion / g, 0.12 g / mL of Bacillus megaterium with an effective viable count of 10 billion / g, and 0.04 g / mL of Candida utilis with an effective viable count of 20 billion / g to the fermentation liquid, mixing well, and obtaining a composite microorganism;

[0044] Step 3, preparing a modified water-retaining agent: 9 parts by weight of grapefruit endocarp powder is placed in a 15% by weight aqueous solution of hydrogen peroxide and soaked for 2 hours, 1.3 parts by weight of behenamidopropyl dimethylamine and 28 parts by weight of acrylic acid are added and mixed to obtain a mixed solution; a 25% by weight aqueous solution of potassium hydroxide is added to the mixed solution to adjust the pH of the mixed solution to 8.0, 0.2 parts by weight of N,N-methylenebisacrylamide and 0.13 parts by weight of potassium persulfate are added, the mixture is stirred evenly, and then placed at 65° C. for reaction, and after the reaction is completed, 35 parts by weight of a mixed solution of sodium alginate (9% by weight) and polyvinyl alcohol (4% by weight) are added, and the mixture is placed at 100° C. and stirred until it becomes viscous, dried, and crushed to obtain a modified water-retaining agent;

[0045] Step 4, 13 parts by weight of the obtained composite microorganisms, 12 parts by weight of the modified water-retaining agent, 25 parts by weight of the functional additives (containing 5 parts by weight of zeolite powder, 7 parts by weight of oyster shell powder, and 13 parts by weight of potassium humate), and 35 parts by weight of decomposed animal manure are mixed, dried, and crushed to obtain a soil biological organic conditioner.

[0046] Comparative Example 1

[0047] Step 1, raw material preparation: separating the yellow outer peel and the inner peel sponge layer of the grapefruit peel; drying and crushing the inner peel sponge layer to obtain the grapefruit inner peel powder for preparing the modified water-retaining agent; drying the yellow outer peel, distilling to remove volatile components, and crushing the grapefruit outer peel powder for preparing the composite microorganism;

[0048] Step 2, preparing the composite microorganism: weighing each component according to the ratio of 1.3g of grapefruit peel powder, 2.5g of corn flour, 1.2g of glucose, 0.03g of potassium dihydrogen phosphate, and 0.25g of calcium carbonate per 100mL of fermentation medium, adding deionized water to prepare, adjusting the pH to 7.0, and sterilizing before use; inoculating the seed liquid of Streptomyces hygroscopicus with an effective viable count of 3 billion / mL into the above fermentation medium at an inoculation amount of 1.5% of the volume of the fermentation medium, culturing at 25°C for 20h, and obtaining a fermentation liquid containing about 3.3 billion / mL of Streptomyces hygroscopicus; adding to the fermentation liquid, mixing, and obtaining the composite microorganism;

[0049] Step 3, preparing a modified water-retaining agent: 9 parts by weight of grapefruit endocarp powder is placed in a 15% by weight aqueous solution of hydrogen peroxide and soaked for 2 hours, 1.3 parts by weight of behenamidopropyl dimethylamine and 28 parts by weight of acrylic acid are added and mixed to obtain a mixed solution; a 25% by weight aqueous solution of potassium hydroxide is added to the mixed solution to adjust the pH of the mixed solution to 8.0, 0.2 parts by weight of N,N-methylenebisacrylamide and 0.13 parts by weight of potassium persulfate are added, the mixture is stirred evenly, and then placed at 65° C. for reaction, and after the reaction is completed, 35 parts by weight of a mixed solution of sodium alginate (9% by weight) and polyvinyl alcohol (4% by weight) are added, and the mixture is placed at 100° C. and stirred until it becomes viscous, dried, and crushed to obtain a modified water-retaining agent;

[0050] Step 4: 3 parts by weight of the obtained Bacillus subtilis with an effective live bacteria count of 20 billion / g, 1 part by weight of Bacillus megaterium with an effective live bacteria count of 10 billion / g, 12 parts by weight of a modified water retaining agent, 25 parts by weight of a functional additive (containing 5 parts by weight of zeolite powder, 7 parts by weight of oyster shell powder, and 13 parts by weight of potassium humate), and 35 parts by weight of decomposed animal feces are mixed, dried, and crushed to obtain a soil biological organic conditioner.

[0051] Comparative Example 2

[0052] Step 1, preparing a composite microorganism: weighing each component according to a ratio of 2.5g corn flour, 1.2g glucose, 0.03g potassium dihydrogen phosphate, and 0.25g calcium carbonate per 100mL fermentation medium, adding deionized water to prepare, adjusting the pH to 7.0, and sterilizing before use; inoculating a seed solution of Streptomyces hygroscopicus with an effective viable count of 3 billion / mL into the above-mentioned fermentation medium at an inoculation amount of 1.5% of the volume of the fermentation medium, culturing at 25°C for 20h to obtain a fermentation liquid containing an effective viable count of about 3.3 billion / mL of Streptomyces hygroscopicus; adding 0.25g / mL of Bacillus subtilis with an effective viable count of 20 billion / g, 0.12g / mL of Bacillus megaterium with an effective viable count of 10 billion / g, and 0.04g / mL of Candida utilis with an effective viable count of 20 billion / g to the fermentation liquid, mixing well, and obtaining a composite microorganism;

[0053] Step 2: Mix 13 parts by weight of the obtained composite microorganisms, 12 parts by weight of polyacrylamide, 25 parts by weight of functional additives (containing 5 parts by weight of zeolite powder, 7 parts by weight of oyster shell powder, and 13 parts by weight of potassium humate), and 35 parts by weight of decomposed animal feces, dry them, and crush them to obtain a soil biological organic conditioner.

[0054] Comparative Example 3

[0055] Step 1, raw material preparation: separating the yellow outer peel and the inner peel sponge layer of the grapefruit peel; drying and crushing the inner peel sponge layer to obtain the grapefruit inner peel powder for preparing the modified water-retaining agent; drying the yellow outer peel, distilling to remove volatile components, and crushing the grapefruit outer peel powder for preparing the composite microorganism;

[0056] Step 2, preparing a composite microorganism: weighing each component according to a ratio of 1.3 g of grapefruit peel powder, 2.5 g of corn flour, 1.2 g of glucose, 0.03 g of potassium dihydrogen phosphate, and 0.25 g of calcium carbonate per 100 mL of fermentation medium, adding deionized water to prepare, adjusting the pH to 7.0, and sterilizing before use; inoculating a seed solution of Streptomyces hygroscopicus with an effective viable count of 3 billion / mL into the above-mentioned fermentation medium at an inoculation amount of 1.5% of the volume of the fermentation medium, culturing at 25° C. for 20 hours, and obtaining a fermentation liquid containing about 3.3 billion / mL of Streptomyces hygroscopicus with an effective viable count of about 3.3 billion / mL; adding 0.25 g / mL of Bacillus subtilis with an effective viable count of 20 billion / g, 0.12 g / mL of Bacillus megaterium with an effective viable count of 10 billion / g, and 0.04 g / mL of Candida utilis with an effective viable count of 20 billion / g to the fermentation liquid, mixing well, and obtaining a composite microorganism;

[0057] Step 3, preparing a modified water-retaining agent: 9 parts by weight of grapefruit endocarp powder is placed in a 15% by weight aqueous hydrogen peroxide solution and soaked for 2 hours, 28 parts by weight of acrylic acid is added and mixed to obtain a mixed solution; a 25% by weight potassium hydroxide aqueous solution is added to the mixed solution to adjust the pH of the mixed solution to 8.0, 0.2 parts by weight of N,N-methylenebisacrylamide and 0.13 parts by weight of potassium persulfate are added, the mixture is stirred evenly, and then placed at 65° C. for reaction, and after the reaction is completed, 35 parts by weight of a 9% sodium alginate solution is added, and the mixture is placed at 100° C. and stirred until it becomes viscous, dried, and crushed to obtain a modified water-retaining agent;

[0058] Step 4, 13 parts by weight of the obtained composite microorganisms, 12 parts by weight of the modified water-retaining agent, 25 parts by weight of oyster shell powder, and 35 parts by weight of decomposed animal feces are mixed, dried, and crushed to obtain a soil biological organic conditioner.

[0059] Performance Testing

[0060] 1. The improvement of acidic soil by soil conditioners and their impact on crops

[0061] 1. Basic Overview

[0062] This experiment was conducted in our company's greenhouse. The basic soil conditions were: organic matter 18.36g / kg, total nitrogen 1.65g / kg, available phosphorus 93.57mg / kg, available potassium 143.9mg / kg, exchangeable total acid content 1.35cmol / kg, exchangeable Al 3+ The content is 0.46cmol / kg and the pH value is 5.18.

[0063] 2. Experimental Design

[0064] The experiment has 7 treatments, including application of the soil conditioner obtained in Example 1 to Example 3, application of the soil conditioner obtained in Comparative Example 1 to Comparative Example 3, and no application of soil conditioner. The dosage of different soil conditioners is 10g / kg soil, and the application method is to mix the soil conditioner with the soil and put it into the seedling pot. Spinach seedlings with basically the same growth are transplanted into the seedling pot, 1 plant / pot, 50 pots for each treatment, and repeated 2 times. The field management during spinach planting is conventional field management.

[0065] 3. Detection indicators

[0066] 30 days after the spinach was transplanted, 10 pots were randomly selected from each treatment to measure the soil pH and soil conditions, and the growth of spinach.

[0067] 4. Results and Analysis

[0068] As shown in Table 1 below, the application of the soil conditioner of the present invention can significantly reduce soil acidity, increase the organic matter content, and reduce the exchangeable acid and exchangeable Al in the soil. 3+ The content.

[0069] Table 1 Comparison of the improvement of acidified soil by different soil conditioners

[0070] Processing Soil pH Exchangeable acid (cmol / kg) <![CDATA[Exchangeable Al 3+ (cmol / kg)]]> Organic matter (g / kg) No treatment 5.11 1.29 0.51 17.95 Example 1 6.32 0.72 0.32 26.54 Example 2 6.01 0.76 0.29 25.12 Example 3 6.15 0.68 0.25 28.75 Comparative Example 1 5.46 0.85 0.43 21.45 Comparative Example 2 5.29 0.91 0.45 22.03 Comparative Example 3 5.53 1.03 0.39 20.67

[0071] As shown in the results in Table 2 below, the application of the soil conditioner of the present invention to the soil for growing spinach can significantly promote the growth of spinach and increase the yield of spinach.

[0072] Table 2 Effects of different soil treatments on spinach growth

[0073]

[0074]

[0075] 2. The improvement of saline-alkali soil by soil conditioners and their impact on crops

[0076] 1. Basic Overview

[0077] This experiment was conducted at our company's cooperative agricultural planting base. The basic soil conditions were: mild salinization, organic matter 13.25g / kg, total nitrogen 2.03g / kg, available phosphorus 62.58mg / kg, available potassium 95.26mg / kg, and pH 8.01.

[0078] 2. Experimental Design

[0079] The experiment set up 7 treatments, including application of the soil conditioner obtained in Example 1 to Example 3, application of the soil conditioner obtained in Comparative Example 1 to Comparative Example 3, and no application of soil conditioner. The dosage of different soil conditioners was 500 kg / hm 2 The application method is to mix the soil conditioner with the tillage layer soil during land preparation. The test crop is corn, and the planting density is 60,000 plants / hm2 2 Corn was planted 10 days after soil conditioner application. The area of ​​each treatment plot was 50m 2 , arranged in random blocks. Other field management was carried out according to routine production.

[0080] 3. Detection indicators

[0081] Soil pH was measured before corn sowing and after corn harvest, and the salt content of the cultivated layer soil was measured by conductivity method. After corn harvest, 10 plants were randomly selected from each treatment to measure their economic traits such as plant height and ear length, as well as overall yield.

[0082] 4. Results and Analysis

[0083] As shown in Table 3 below, the application of the soil conditioner of the present invention can significantly reduce the salt content in the soil and lower the pH value. After the corn is harvested, the salt content does not change significantly, indicating that the soil conditioner of the present invention can slow down the surface accumulation of salt in the soil.

[0084] Table 3 Comparison of the improvement of saline-alkali soil under different soil treatment conditions

[0085]

[0086] From the results in Table 4, it can be seen that the land improved by the soil conditioner of the present invention can significantly increase the yield of corn, and the overall properties of corn are good, and indicators such as corn plant height and ear length are significantly increased.

[0087] Table 4 Effects of different soil treatments on maize traits

[0088] Processing Plant height (cm) Ear length (cm) Number of grains per spike 100-grain mass (g) <![CDATA[Yield (kg / hm 2 )]]> No treatment 175.5 12.9 269 21.6 3965.8 Example 1 183.2 14.6 306 25.1 4562.1 Example 2 182.6 14.3 335 25.8 4395.6 Example 3 184.1 14.5 319 24.3 4425.8 Comparative Example 1 180.2 13.3 291 22.3 4136.8 Comparative Example 2 179.6 13.6 282 22.8 4098.6 Comparative Example 3 178.5 13.9 293 23.5 4218.2

Claims

1. A soil biological organic conditioner for treating acid and alkali, characterized in that: The invention is prepared from the following components: 10-15 parts by weight of composite microorganisms, 10-15 parts by weight of modified water retaining agent, 15-30 parts by weight of functional additives and 30-40 parts by weight of decomposed animal excrement.

2. The soil biological organic conditioner according to claim 1, characterized in that: The preparation method of the soil biological organic conditioner comprises the following steps: Step 1, raw material preparation: separating the yellow outer peel and the inner peel sponge layer of the grapefruit peel; drying and crushing the inner peel sponge layer to obtain the grapefruit inner peel powder for preparing the modified water-retaining agent; drying the yellow outer peel, distilling to remove volatile components, and crushing the grapefruit outer peel powder for preparing the composite microorganism; Step 2, preparing the composite microorganism: inoculating the seed liquid of Streptomyces hygroscopicus with acanthosporium into a fermentation medium prepared by grapefruit exocarp powder, corn flour, glucose, potassium dihydrogen phosphate, and calcium carbonate, and culturing at 25-30° C. for 20-24 hours to obtain a fermentation liquid containing Streptomyces hygroscopicus with acanthosporium; adding Bacillus subtilis, Bacillus megaterium, and Candida utilis to the fermentation liquid, and mixing well to obtain the composite microorganism; Step 3, preparing a modified water-retaining agent: soaking grapefruit endocarp powder in a hydrogen peroxide aqueous solution for 1.5-2 hours, adding behenamidopropyl dimethylamine and acrylic acid and mixing to obtain a mixed solution; adding potassium hydroxide aqueous solution to the mixed solution to adjust the pH of the mixed solution to 7.5-8.0, adding N,N-methylenebisacrylamide and potassium persulfate, stirring evenly, and reacting at 60-65° C., adding a mixed solution of sodium alginate and polyvinyl alcohol after the reaction is completed, stirring at 90-100° C. until viscous, drying, and crushing to obtain a modified water-retaining agent; Step 4, mixing the obtained composite microorganisms, modified water-retaining agent, functional additives and decomposed animal feces, drying and crushing them to obtain a soil biological organic conditioner.

3. The soil biological organic conditioner according to claim 2, characterized in that: In the preparation of the composite microorganism in step 2, each 100 mL of fermentation medium contains 1-1.5 g of grapefruit peel powder, 2-3 g of corn flour, 1-1.5 g of glucose, 0.01-0.05 g of potassium dihydrogen phosphate, and 0.2-0.3 g of calcium carbonate. The medium is prepared with deionized water, the pH is adjusted to 7.0, and the medium is sterilized before use.

4. The soil biological organic conditioner according to claim 2, characterized in that: In the preparation of the composite microorganism in step 2, the inoculation amount of the seed solution of Streptomyces hygroscopicus is 1-1.5% of the volume of the fermentation medium; the effective viable count of the seed solution of Streptomyces hygroscopicus is 3 billion / mL.

5. The soil biological organic conditioner according to claim 2, characterized in that: In the step 2 of preparing the composite microorganism, the effective viable bacteria count of Streptomyces hygroscopicus in the obtained fermentation broth is 2-4 billion / mL.

6. The soil biological organic conditioner according to claim 2, characterized in that: In the preparation of the composite microorganism in step 2, the added amount of Bacillus subtilis is 0.2-0.3 g / mL, the added amount of Bacillus megaterium is 0.1-0.15 g / mL, and the added amount of Candida utilis is 0.03-0.05 g / mL; the effective live bacteria count of Bacillus subtilis is 20 billion / g, the effective live bacteria count of Bacillus megaterium is 10 billion / g, and the effective live bacteria count of Candida utilis is 20 billion / g.

7. The soil biological organic conditioner according to claim 2, characterized in that: In the preparation of the modified water-retaining agent in step 3, the usage amounts of the components are: 7-10 parts by weight of grapefruit endocarp powder, 1-1.5 parts by weight of behenamidopropyl dimethylamine, 25-30 parts by weight of acrylic acid, 0.15-0.25 parts by weight of N,N-methylenebisacrylamide, and 0.1-0.15 parts by weight of potassium persulfate.

8. The soil biological organic conditioner according to claim 2, characterized in that: In the preparation of the modified water-retaining agent in step 3, the hydrogen peroxide aqueous solution is a hydrogen peroxide solution with a mass fraction of 15%, and the potassium hydroxide aqueous solution is a potassium hydroxide aqueous solution with a mass fraction of 25%-30%.

9. The soil biological organic conditioner according to claim 2, characterized in that: In the preparation of the modified water-retaining agent in step 3, the amount of the mixed solution of sodium alginate and polyvinyl alcohol used is 30-40 parts by weight; the mass fraction of sodium alginate in the mixed solution is 8%-10%, and the mass fraction of polyvinyl alcohol is 3%-5%.

10. The soil biological organic conditioner according to any one of claims 1 to 9, characterized in that: The functional additive contains 2-7 parts by weight of zeolite powder, 3-8 parts by weight of oyster shell powder and 10-15 parts by weight of potassium humate.

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