Preparation method and application of sea cucumber peptide

Abstract

The invention relates to the technical field of medical care, and particularly discloses a preparation method and application of sea cucumber peptide. The preparation method of the sea cucumber peptide provided by the invention comprises the following steps: heating sea cucumber primary pulp for denaturation, and then adding compound protease for enzymolysis treatment to obtain sea cucumber enzymatic hydrolysate; the addition amount of the compound protease is 0.1-0.15 mg / mL, and the compound protease is prepared from papain, ficin and trypsin according to the weight ratio of 1: (0.5-1.5): (2-3); adding activated carbon and diatomite into the sea cucumber enzymatic hydrolysate, and performing enzyme deactivation, filtration and concentration treatment to obtain sea cucumber peptide; the invention also provides application of the sea cucumber peptide obtained by the preparation method in medicines and foods. According to the preparation method of the sea cucumber peptide, the sea cucumber peptide with small molecular weight and good uniformity can be obtained, and the prepared sea cucumber peptide tablet candy has the effects of relieving physical fatigue, resisting aging, resisting oxidation and the like.

Description

Technical Field

[0001] The present application relates to the field of medical care technology, and in particular to a method for preparing a sea cucumber peptide and its application. Background Art

[0002] Sea cucumbers are not only rich in more than 50 kinds of nutrients needed by the human body, such as amino acids, vitamins and chemical elements, but also contain a variety of bioactive substances such as acid mucopolysaccharides, saponins and collagen, etc. These active substances have a wide range of pharmacological activities, such as delaying human aging, lowering blood pressure, anti-tumor, preventing cardiovascular and cerebrovascular diseases, improving immunity, beauty and other effects. In particular, bioactive peptides have become one of the hottest research topics in the food industry because of their various physiological regulatory functions, such as improving immunity, hormone regulation, anti-oxidation, antibacterial, antiviral, lowering blood pressure, and lowering blood lipids.

[0003] In order to make full use of the active ingredients in sea cucumbers, the existing technology mainly uses enzymatic hydrolysis to prepare sea cucumber peptides. The specific method is to first use a single or composite protease to hydrolyze the sea cucumber to decompose the sea cucumber protein macromolecules into small polypeptide molecules, and then purify and separate them by physical and chemical methods to obtain sea cucumber peptides. However, in the actual operation process, due to improper selection of protease types and hydrolysis conditions, the decomposed polypeptide chains have problems such as high molecular weight or different molecular weight lengths and poor uniformity, which in turn affects the activity and absorption efficiency of sea cucumber peptides in subsequent product applications. Summary of the invention

[0004] In order to overcome the problems of high molecular weight or uneven molecular weight length and poor uniformity of polypeptide chains in the existing enzymatic hydrolysis method for preparing sea cucumber peptides, the present application provides a method for preparing sea cucumber peptides and its application.

[0005] In the first aspect, the preparation method of sea cucumber peptide provided in the present application adopts the following technical scheme: A method for preparing sea cucumber peptide comprises the following steps: The sea cucumber pulp is heated and denatured, and then a composite protease is added for enzymatic hydrolysis to obtain a sea cucumber enzymatic hydrolysate; the amount of the composite protease added is 0.1-0.15 mg / mL, and the composite protease is composed of papain, ficin and trypsin in a weight ratio of 1: (0.5-1.5): (2-3); Activated carbon and diatomaceous earth are added to the sea cucumber hydrolysate, and the sea cucumber peptide is obtained after the enzyme is inactivated, filtered and concentrated.

[0006] The present application provides a method for preparing sea cucumber peptides, which first denatures the sea cucumber pulp by heating it, so that the denatured structure of the sea cucumber collagen unfolds to facilitate subsequent enzymatic hydrolysis, and then enzymatically hydrolyzes the sea cucumber into small molecule active peptides by using a certain ratio of composite protease, which is convenient for biological utilization and absorption, so that the value of the sea cucumber can be fully utilized; finally, the miscellaneous color and fishy smell in the sea cucumber peptides are further removed through decolorization and deodorization treatment, the fishy smell of the sea cucumber peptides is reduced, and the overall flavor of the sea cucumber peptide-related application products is improved. The content of small molecule active peptides (weight average molecular weight less than 2000Da) in the sea cucumber peptides obtained by the preparation method of the sea cucumber peptides provided in the present application can reach more than 80%, which effectively solves the problems of high molecular weight and poor uniformity after enzymatic hydrolysis of sea cucumbers in the prior art. The sea cucumber peptides obtained by the preparation method of the sea cucumber peptides provided in the present application are used to prepare health foods related to relieving physical fatigue, anti-aging, enhancing immunity, and anti-oxidation, and have good application prospects.

[0007] In some embodiments, the weight ratio of papain, ficin and trypsin can be 1: (0.5-0.8): 2.5, 1: (0.5-1): 2.5, 1: (0.5-1.3): 2.5, 1: (0.5-1.5): 2.5, 1: (0.8-1): 2.5, 1: (0.8-1.3): 2.5, 1: (0.8-1.5): 2.5, 1: (1-1.3): 2.5, 1: (1-1.5): 2.5, 1: (1.3-1.5): 2.5, 1:1:(2-2.2), 1:1:(2-2.5), 1:1:(2-2.8), 1:1:(2-3), 1:1:(2.2-2.5), 1:1:(2.2-2.8), 1:1:(2.2-3), 1:1:(2.5-2.8), 1:1:(2.5-3) or 1:1:(2.8-3).

[0008] In a specific embodiment, the weight ratio of papain, ficin and trypsin can be 1:0.5:2.5, 1:0.8:2.5, 1:1:2.5, 1:1.3:2.5, 1:1.5:2.5, 1:1:2, 1:1: 2.2, 1:1:2.8 or 1:1:3.

[0009] In some embodiments, the added amount of the composite protease can be 0.1-0.13 mg / mL or 0.13-0.15 mg / mL.

[0010] In a specific embodiment, the added amount of the composite protease can be 0.1 mg / mL, 0.13 mg / mL or 0.15 mg / mL.

[0011] Optionally, the weight ratio of papain, ficin and trypsin is 1:(0.8-1.3):(2.2-2.8).

[0012] Optionally, the enzymatic activity of papain is ≥10000 U / g, the enzymatic activity of ficin is ≥1000 U / g, and the enzymatic activity of trypsin is 1000000-2000000 U / g.

[0013] Optionally, the pH of the sea cucumber puree is 7.0-8.0; the enzymolysis temperature is 30-45°C, and the enzymolysis time is 2-12h.

[0014] Optionally, the added amount of the activated carbon is 10-15wt% of the sea cucumber enzymatic hydrolysate, and the added amount of the diatomaceous earth is 5-10wt% of the sea cucumber enzymatic hydrolysate.

[0015] Optionally, the specific steps of the heat denaturation treatment are: heating the sea cucumber puree to 115-125°C, maintaining the temperature for 20-25 minutes, and then cooling to 35-40°C.

[0016] In a second aspect, the present application provides a method for preparing a sea cucumber peptide and obtaining the sea cucumber peptide.

[0017] In a third aspect, the present application provides a method for preparing sea cucumber peptides and the use of sea cucumber peptides obtained in medicines and foods.

[0018] A sea cucumber peptide tablet candy comprises the following components in parts by weight: 10-15 parts of sea cucumber peptide, 30-40 parts of sorbitol, 1-3 parts of magnesium stearate, 0.5-1.5 parts of complex vitamins, 0.1-0.5 parts of nicotinamide, 0.1-0.5 parts of beta-cyclodextrin and 0.1-0.5 parts of sodium carboxymethyl cellulose.

[0019] In some embodiments, the weight portion of the sea cucumber peptide may be 10-13 parts or 13-15 parts.

[0020] In a specific embodiment, the weight portion of the sea cucumber peptide can also be 10 parts, 13 parts or 15 parts.

[0021] The vitamin complex is selected from vitamin A, vitamin D3, vitamin E, vitamin C, vitamin B1, vitamin B2, vitamin B6 and vitamin B 12 One or more of .

[0022] In summary, this application has the following beneficial effects: 1. The present application provides a method for preparing sea cucumber peptides. In the preparation method, papain, ficin and trypsin in a weight ratio of 1: (0.5-1.5): (2-3) are used as a composite protease to enzymatically hydrolyze sea cucumber puree, and the amount of the composite protease added is controlled within the range of 0.1-0.15 mg / mL. The finally obtained sea cucumber peptide has a small molecular weight and high uniformity. The content of small molecular active peptides (weight-average molecular weight less than 2000 Da) in the sea cucumber peptide can reach more than 80%, which effectively solves the problems of high molecular weight and poor uniformity after sea cucumber enzymatic hydrolysis in the prior art.

[0023] 2. The sea cucumber peptide obtained by the preparation method of the sea cucumber peptide provided in the present application has a small molecular weight and is easy to be biologically utilized and absorbed. The sea cucumber peptide compressed candy prepared by the method has good effects of relieving physical fatigue, anti-aging, enhancing immunity, and anti-oxidation.

[0024] 3. The present application further controls the weight ratio of papain, ficin and trypsin in the composite protease within the range of 1:(0.8-1.3):(2.2-2.8), and the obtained sea cucumber peptide has a smaller molecular weight and higher uniformity. The sea cucumber peptide compressed candy prepared by the composite protease has better effects in relieving physical fatigue and strengthening the kidney. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a flow chart of the preparation method of sea cucumber peptide provided in this application. DETAILED DESCRIPTION

[0026] The present application provides a method for preparing sea cucumber peptide, comprising the following steps: (1) heating the sea cucumber puree to 115-125° C., maintaining the temperature for 20-25 min, and cooling to 35-40° C.; then adding a composite protease for enzymatic hydrolysis, wherein the amount of the composite protease added is 0.1-0.15 mg / mL, the enzymatic hydrolysis temperature is 30-45° C., and the enzymatic hydrolysis time is 2-12 h to obtain a sea cucumber hydrolysate; the composite protease is composed of papain, ficin and trypsin in a weight ratio of 1: (0.5-1.5): (2-3); further, the weight ratio of the papain, ficin and trypsin is 1: (0.8-1.3): (2.2-2.8); (2) Adding activated carbon and diatomaceous earth to the sea cucumber hydrolysate for decolorization and deodorization, and then killing the enzyme, filtering and concentrating to obtain sea cucumber peptides; wherein the amount of activated carbon added is 10-15wt% of the sea cucumber hydrolysate, and the amount of diatomaceous earth added is 5-10wt% of the sea cucumber hydrolysate.

[0027] The present application provides a method for preparing a sea cucumber slice compressed candy, comprising the following steps: mixing sea cucumber peptide, sorbitol, complex vitamins, nicotinamide, β-cyclodextrin and sodium carboxymethyl cellulose at a rotation speed of 30-40 Hz for 10-20 minutes, then adding magnesium stearate, mixing at a rotation speed of 30-40 Hz for 10-20 minutes, and then compressing to obtain an anti-aging compressed candy; wherein the complex vitamins are selected from vitamin A, vitamin D3, vitamin E, vitamin C, vitamin B1, vitamin B2, vitamin B6 and vitamin B 12 One or more of .

[0028] In the present application, the sea cucumber puree is obtained by removing the internal organs, washing, drying and homogenizing fresh Icelandic red sea cucumber, and its pH is 7.5; papain, ficin and trypsin are all purchased from Merck, and the enzyme activity of each protease is ≥10000U / g, ≥1000U / g, and 1000000-2000000U / g, respectively. The raw materials, reagents, solvents, etc. used in the present application can all be obtained commercially.

[0029] The present application is further described in detail below in combination with embodiments, performance testing and accompanying drawings.

[0030] Preparation Example 1-9 Preparation Examples 1-9 respectively provide a method for preparing a sea cucumber peptide.

[0031] The difference between the above preparation examples is that the ratio of the composite protease is shown in Table 1 below.

[0032] The preparation method of sea cucumber peptide provided in Preparation Example 1-9 comprises the following steps: (1) heating the sea cucumber puree to 121° C., maintaining the temperature for 25 min, and cooling to 40° C.; then adding a composite protease for enzymatic hydrolysis, wherein the amount of the composite protease added is 0.13 mg / mL, the enzymatic hydrolysis temperature is 45° C., and the enzymatic hydrolysis time is 8 h to obtain a sea cucumber enzymatic hydrolyzate; (2) Adding activated carbon and diatomaceous earth to the sea cucumber hydrolysate for decolorization and deodorization, and obtaining sea cucumber peptides after inactivating the enzyme, filtering and concentrating; wherein the amount of activated carbon added is 13wt% of the sea cucumber hydrolysate, and the amount of diatomaceous earth added is 8wt% of the sea cucumber hydrolysate.

[0033] Table 1 Proportions of the composite protease in the preparation methods provided in Preparation Examples 1-9 Preparation Example 10 Preparation Example 10 provides a method for preparing sea cucumber peptide.

[0034] The difference between the above preparation example and preparation example 3 is that the added amount of the composite protease is 0.1 mg / mL.

[0035] Preparation Example 11 Preparation Example 11 provides a method for preparing sea cucumber peptide.

[0036] The difference between the above preparation example and preparation example 3 is that the added amount of the composite protease is 0.15 mg / mL.

[0037] Comparative Preparation Example 1 Comparative Preparation Example 1 provides a method for preparing sea cucumber peptide.

[0038] The difference between the comparative preparation example and preparation example 3 is that the composite protease is composed of papain, ficin and trypsin in a weight ratio of 1:2:1.

[0039] Comparative Preparation Example 2 Comparative Preparation Example 2 provides a method for preparing sea cucumber peptide.

[0040] The difference between the comparative preparation example and preparation example 3 is that the composite protease is composed of papain, neutral protease and trypsin in a weight ratio of 1:1:2.5.

[0041] Comparative Preparation Example 3 Comparative Preparation Example 3 provides a method for preparing sea cucumber peptide.

[0042] The difference between the comparative preparation example and preparation example 3 is that the composite protease is composed of papain, flavor protease, neutral protease and trypsin in a weight ratio of 1:1:1:1.

[0043] Comparative Preparation Example 4 Comparative Preparation Example 4 provides a method for preparing sea cucumber peptide.

[0044] The difference between the comparative preparation example and preparation example 3 is that the added amount of the composite protease is 0.05 mg / mL.

[0045] Molecular weight detection The weight average molecular weight of the sea cucumber peptides obtained in Preparation Example 1-11 and Comparative Preparation Example 1-4 was tested, and the results are shown in Table 2 below.

[0046] Table 2 The weight average molecular weight test results of sea cucumber peptides obtained in Preparation Examples 1-11 and Comparative Preparation Examples 1-4 According to the test results in Table 2, the content of small molecule active peptides with a weight average molecular weight of less than 2000Da in the sea cucumber peptides obtained in Preparation Examples 1-11 can reach more than 80%; while the content of small molecule active peptides with a weight average molecular weight of less than 2000Da in the sea cucumber peptides obtained in Comparative Preparation Examples 1-4 is only more than 48.8-73.6%. Therefore, it is shown that the preparation method of the provided sea cucumber peptide can obtain sea cucumber peptides with small molecular weight and good uniformity, and the sea cucumber peptide products prepared by the method can be convenient for biological utilization and absorption.

[0047] Embodiment 1-11 Examples 1-11 each provide a sea cucumber peptide tablet candy.

[0048] The difference between the above embodiments is that the sea cucumber peptides in the sea cucumber peptide compressed candies are derived from preparation examples 1-11 respectively.

[0049] The preparation method of the sea cucumber peptide compressed candy provided in Example 1-11 comprises the following steps: 13 g of sea cucumber peptide, 35 g of sorbitol, 0.4 g of vitamin E, 0.4 g of vitamin C, 0.2 g of vitamin B1, 0.3 g of nicotinamide, 0.3 g of β-cyclodextrin and 0.3 g of sodium carboxymethyl cellulose are weighed respectively, mixed at a speed of 40 Hz for 15 min, then 2 g of magnesium stearate is added, and the mixture is mixed at a speed of 30-40 Hz for 10-20 min, and then pressed into shape to obtain anti-aging compressed candy.

[0050] Example 12 Example 12 provides a sea cucumber peptide compressed candy.

[0051] The difference between the above embodiment and embodiment 3 is that the added amount of sea cucumber peptide is 10 g.

[0052] Example 13 Example 13 provides a sea cucumber peptide compressed candy.

[0053] The difference between the above embodiment and embodiment 3 is that the added amount of sea cucumber peptide is 15g.

[0054] Comparative Examples 1-4 Comparative Examples 1-4 respectively provide a kind of sea cucumber peptide tablet candy.

[0055] The difference between the above comparative example and Example 3 is that the sea cucumber peptides in the sea cucumber peptide compressed candies are derived from comparative preparation examples 1-4 respectively.

[0056] Comparative Example 5 Comparative Example 5 provides a sea cucumber peptide tablet candy.

[0057] The difference between the comparative example and Example 3 is that the added amount of sea cucumber peptide is 5 g.

[0058] Performance testing The performance of the sea cucumber peptide tablet candies obtained in Examples 1-13 and Comparative Examples 1-5 was tested through fatigue relief tests and kidney tonifying tests. The results are shown in Table 3 below.

[0059] (I) Fatigue relief test 1. Experimental subjects: 190 female Kunming aged mice aged 10 months, weighing 55±5g; randomly divided into 19 groups (experimental groups 1-13, control groups 1-5 and blank group), with 10 mice in each group; 2. Test methods: (1) Oral gavage: The sea cucumber peptide tablet candy was crushed and dissolved with an appropriate amount of water, and then the mice were gavaged. The gavage amount of the sea cucumber peptide tablet candy was 0.55 g / kg, once a day, for 4 consecutive weeks; during the test period, the animals in each group were free to drink 0.1% BSA acidified water; among them, the mice in the test groups 1-13 were gavaged with the sea cucumber peptide tablet candies of Examples 1-13, the mice in the control groups 1-5 were gavaged with the sea cucumber peptide tablet candies of Comparative Examples 1-5, and the blank group was gavaged with physiological saline; (2) Fatigue test: After the above-mentioned gavage test, the mice were starved for 24 hours, and then gavage was performed again. One hour later, the mice were placed in a 27±2℃ water pool, and the survival time of the mice in the water was recorded, that is, the time from the mouse entering the water to the mouth and nose sinking into the water for more than 10 seconds. The survival time of the mice in the water was used to measure the fatigue resistance of the mice, and then to characterize the fatigue resistance of the sea cucumber peptide compressed candy.

[0060] (II) Kidney-tonifying test 1. Experimental subjects: 200 male SD rats, weighing 200±10g. Among them, 190 rats were anesthetized by injection of 12.5% ​​urethane 0.6mL / rat, the scrotal skin was disinfected, the testicles were removed, and penicillin sodium 20,000U / kg was injected intramuscularly for 3 consecutive days. The rats after the above treatment were randomly divided into 19 groups (experimental groups 1-13, control groups 1-5 and blank groups), with 10 rats in each group; 10 rats were not treated and served as the normal group; 2. Test methods: (1) Oral gavage: The sea cucumber peptide tablet candy was crushed and dissolved with an appropriate amount of water, and then the rats were gavaged. The gavage dose of the sea cucumber peptide tablet candy was 0.35 g / kg, once a day for 30 consecutive days; The mice in the test groups 1-13 were gavaged with the sea cucumber peptide tablet candies of Examples 1-13, the mice in the control groups 1-5 were gavaged with the sea cucumber peptide tablet candies of Comparative Examples 1-5, and the mice in the blank group and the normal group were gavaged with physiological saline; (2) Dissection: 24 hours after the last oral administration, the rats were weighed, then killed and dissected, and the seminal vesicles, prostate glands, and preputial glands were taken, weighed, and the organ coefficients were calculated and the average value was taken. Note: The organ coefficient refers to the organ weight per unit weight of the rat.

[0061] Table 3 Performance test results of sea cucumber peptide tablet candies obtained in Examples and Comparative Examples According to the test results of Table 3, after the test group 1-13 used the sea cucumber peptide tablet candy obtained in Examples 1-13 of the present application to gavage mice for a period of time, the anti-fatigue survival time of the mice can reach about 2597-2915s, the organ coefficients of the seminal vesicle and prostate of the mice can reach about 82.27-103.11mg / 100g, and the organ coefficient of the preputial gland can reach about 45.91-63.57mg / 100g; while the blank group used normal saline to gavage mice for a period of time, the anti-fatigue survival time of the mice was only about 1237s, the organ coefficients of the seminal vesicle and prostate of the mice were only about 24.56mg / 100g, and the organ coefficient of the preputial gland was only about 17.33mg / 100g. Therefore, it is explained that the sea cucumber peptide tablet candy provided by the present application has the effect of relieving physical fatigue and strengthening the kidney.

[0062] From the results of the experimental groups 1-11 and the control groups 1-4, it can be seen that the anti-fatigue survival time of the mice in the experimental groups 1-11 can reach about 2624-2915s, the organ coefficients of the seminal vesicles and prostate of the mice can reach about 87.45-103.11mg / 100g, and the organ coefficient of the foreskin gland can reach about 47.85-63.57mg / 100g; the anti-fatigue survival time of the mice in the control groups 1-4 is only about 1824-2312s, the organ coefficients of the seminal vesicles and prostate of the mice are only about 66.26-76.37mg / 100g, and the organ coefficient of the foreskin gland is only about 23.34-34.21mg / 100g. Therefore, it is explained that the present application adopts papain, ficin and trypsin in a weight ratio of 1: (0.5-1.5): (2-3) as the composite protease, and the addition amount of the composite protease is controlled within the range of 0.1-0.15 mg / mL. The obtained sea cucumber peptide has a small molecular weight and high uniformity. The sea cucumber peptide compressed candy prepared by using the composite protease has good effects in relieving physical fatigue and strengthening the kidney. Further comparison of test groups 1-9 revealed that the anti-fatigue survival time of mice in test groups 2-4 and test groups 7-8 could reach about 2773-2915s (≥2700s), the organ coefficients of the seminal vesicle and prostate of mice could reach about 95.30-103.11mg / 100g (≥95mg / 100g), and the organ coefficient of the foreskin gland could reach about 56.21-63.57mg / 100g (≥55mg / 100g). Therefore, it is shown that the present application further controls the weight ratio of papain, ficin and trypsin within the range of 1:(0.8-1.3):(2.2-2.8), and the obtained sea cucumber peptide has a smaller molecular weight and higher uniformity, and the sea cucumber peptide tablet candy prepared therefrom has better effects in relieving physical fatigue and strengthening the kidney.

[0063] The test results of test group 3, test group 12-13, and control group 5 show that the anti-fatigue survival time of mice in test group 3 and test group 12-13 can reach about 2597-2915s, the organ coefficients of the seminal vesicle and prostate of mice can reach about 82.27-103.11mg / 100g, and the organ coefficient of the preputial gland can reach about 45.91-63.57mg / 100g; the anti-fatigue survival time of mice in control group 5 is only about 1896s, the organ coefficients of the seminal vesicle and prostate of mice are only about 67.69mg / 100g, and the organ coefficient of the preputial gland is only about 25.11mg / 100g. Therefore, it is explained that the present application controls the weight of sea cucumber peptide in sea cucumber peptide tablet candy within the range of 10-15 parts, and the obtained sea cucumber peptide tablet candy has good effects on relieving physical fatigue and strengthening the kidney.

[0064] Although the present invention has been described in detail above with general descriptions and specific embodiments, it is obvious to those skilled in the art that some modifications or improvements may be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A method for preparing sea cucumber peptide, characterized in that: The steps include: The sea cucumber pulp is heated and denatured, and then a composite protease is added for enzymatic hydrolysis to obtain a sea cucumber enzymatic hydrolysate; the amount of the composite protease added is 0.1-0.15 mg / mL, and the composite protease is composed of papain, ficin and trypsin in a weight ratio of 1: (0.5-1.5): (2-3); Activated carbon and diatomaceous earth are added to the sea cucumber hydrolysate, and the sea cucumber peptide is obtained after the enzyme is inactivated, filtered and concentrated.

2. The method for preparing sea cucumber peptide according to claim 1, characterized in that: The weight ratio of papain, ficin and trypsin is 1:(0.8-1.3):(2.2-2.8).

3. The method for preparing sea cucumber peptide according to claim 1, characterized in that: The enzymatic activity of the papain is ≥10000 U / g, the enzymatic activity of the ficin is ≥1000 U / g, and the enzymatic activity of the trypsin is 1000000-2000000 U / g.

4. The method for preparing sea cucumber peptide according to claim 1, characterized in that: The pH of the sea cucumber puree is 7.0-8.0; the enzymolysis temperature is 30-45° C., and the enzymolysis time is 2-12 hours.

5. The method for preparing sea cucumber peptide according to claim 1, characterized in that: The added amount of the activated carbon is 10-15wt% of the sea cucumber hydrolysate, and the added amount of the diatomaceous earth is 5-10wt% of the sea cucumber hydrolysate.

6. The method for preparing sea cucumber peptide according to claim 1, characterized in that: The specific steps of the heating denaturation treatment are: heating the sea cucumber puree to 115-125°C, maintaining the temperature for 20-25 minutes, and then cooling to 35-40°C.

7. The sea cucumber peptide obtained by the preparation method of the sea cucumber peptide according to any one of claims 1 to 6.

8. Use of the sea cucumber peptide obtained by the preparation method of the sea cucumber peptide according to any one of claims 1 to 6 in medicine and food.

9. A sea cucumber peptide tablet candy, characterized in that: The invention comprises the following components in parts by weight: 10-15 parts of sea cucumber peptide, 30-40 parts of sorbitol, 1-3 parts of magnesium stearate, 0.5-1.5 parts of complex vitamins, 0.1-0.5 parts of nicotinamide, 0.1-0.5 parts of beta-cyclodextrin and 0.1-0.5 parts of sodium carboxymethyl cellulose.

10. The sea cucumber peptide tablet candy according to claim 9, characterized in that: The vitamin complex is selected from vitamin A, vitamin D3, vitamin E, vitamin C, vitamin B1, vitamin B2, vitamin B6 and vitamin B 12 One or more of .

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