Method for increasing content of phenylalanine aroma substances in flue-cured tobacco

Abstract

The invention discloses a method for increasing the content of phenylalanine aroma substances in flue-cured tobacco, which is characterized in that a propyl dihydrojasmonate solution with a certain concentration is uniformly sprayed on the surfaces of tobacco leaves 24 hours before the tobacco leaves are mature and harvested, and the propyl dihydrojasmonate solution is prepared by mixing a propyl dihydrojasmonate stock solution, absolute ethyl alcohol and Tween. The content of phenylalanine aroma substances related to aroma and taste in the tobacco leaves can be increased, so that the smoking quality of the tobacco leaves is improved.

Description

Technical Field

[0001] The invention relates to a method for increasing the content of phenylalanine aroma substances in flue-cured tobacco, and belongs to the technical field of flue-cured tobacco cultivation. Background Art

[0002] The aroma of tobacco leaves is an important indicator of tobacco quality. The quality of tobacco depends largely on the aroma substances in tobacco leaves. Increasing the content of beneficial aroma substances in tobacco leaves has become an eternal topic in flue-cured tobacco cultivation and industrial formulation. Phenylalanine aroma substances have a good effect on the aroma of flue-cured tobacco, especially the fruity and fresh aromas of flue-cured tobacco. Phenylalanine aroma substances give tobacco leaves a special aroma, which is very beneficial in improving the aroma of tobacco.

[0003] The existing document (Announcement No.: CN109804880B) discloses a cultivation and baking method for increasing the phenylalanine content of flue-cured tobacco. The nitrogen control cultivation method in the scheme includes transplanting tobacco seedlings (applying basal fertilizer in holes before transplanting tobacco seedlings, and then transplanting the transplanted seedlings into ponds and watering them to establish roots), intertillage and weeding and gradual ridging (carrying out 2 to 3 intertillage and weeding after the tobacco seedlings are transplanted into the field and gradually ridging until ridging is completed during the intertillage and weeding process), and gradual topdressing (using tobacco-specific compound fertilizer for topdressing). By controlling the amount of nitrogen applied to tobacco leaves in cultivation measures, the synthesis of phenylalanine content in tobacco leaves is effectively increased, thereby increasing the aroma of cured tobacco leaves. Summary of the invention

[0004] Based on the above, the present invention provides a method for spraying a propyl dihydrojasmonate solution before harvesting to increase the content of phenylalanine aroma substances in tobacco leaves.

[0005] The technical scheme of the present invention is: a method for increasing the content of phenylalanine aroma substances in flue-cured tobacco, wherein a certain concentration of propyl dihydrojasmonate solution is sprayed on the surface of tobacco leaves before the tobacco leaves are mature and harvested, and the propyl dihydrojasmonate solution is prepared by mixing propyl dihydrojasmonate stock solution, anhydrous ethanol and Tween.

[0006] The applicant has found that spraying exogenous propyl dihydrojasmonate can catalyze phenylalanine to produce phenylacetaldehyde, phenylethanol, phenylacetic acid and many other phenylalanine aroma substances related to aroma and taste, thereby improving the smoking quality of tobacco leaves.

[0007] Preferably, the preparation method of the propyl dihydrojasmonate solution is: mix 52.63 mL of propyl dihydrojasmonate stock solution with 20 mL of anhydrous ethanol and 4 to 6 drops of Tween, and add clean water to 1 L; when spraying, dilute the propyl dihydrojasmonate solution with clean water by 200 to 800 times.

[0008] Preferably, the tobacco leaves are middle and upper tobacco leaves (generally 3-4 tobacco leaves), and 80mL to 120mL is sprayed on each tobacco leaf.

[0009] Preferably, the dihydrojasmonate solution is sprayed 12 to 24 hours before the tobacco leaves mature and are harvested. The time interval between the spraying of the dihydrojasmonate solution on the leaves to be harvested and the time interval between the spraying of the dihydrojasmonate solution and the harvesting is not less than 12 hours and not more than 24 hours. In addition, it is carried out on a sunny day and before 10:00. After the leaves to be harvested are sprayed with the dihydrojasmonate solution, if there is rainfall within 6 hours that may leach the liquid sprayed on the leaves, it is necessary to spray again.

[0010] Beneficial effects of the present invention: Based on conventional flue-cured tobacco cultivation, the present invention sprays exogenous propyl dihydrojasmonate before tobacco leaves mature and are ready for harvest, catalyzing phenylalanine to produce phenylacetaldehyde, phenylethanol, phenylacetic acid and many other phenylalanine aroma substances related to aroma and taste, thereby improving the smoking quality of tobacco leaves. Overall, compared with the conventional conventional cultivation method, the present invention has the advantages of significantly increasing the content of phenylalanine aroma substances and improving the smoking quality of tobacco leaves. DETAILED DESCRIPTION

[0011] In order to make the above-mentioned purpose, features and advantages of the present invention more obvious and easy to understand, the specific implementation of the present invention is described in detail below. In the following description, many specific details are set forth to facilitate a full understanding of the present invention. However, the present invention can be implemented in many other ways different from those described herein, and those skilled in the art can make similar improvements without violating the connotation of the present invention, so the present invention is not limited by the specific implementation disclosed below.

[0012] Example 1

[0013] Preparation of 5% propyl dihydrojasmonate mother solution: Prepare 52.63 ml of propyl dihydrojasmonate stock solution (purity 95%), 20 ml of anhydrous ethanol, and 5 drops of Tween, and add purified water to 1 L to obtain the mother solution, and store it in a brown bottle away from light for later use.

[0014] In 2023, at the Pingba Experimental Base in Anshun City, Guizhou Province, with clean water treatment as the control, 24 hours before the third kang (middle leaves) were harvested, the leaves to be harvested were sprayed with dihydrojasmonate solution of different dilution multiples, and 100 ml was quantified for each plant. The mother liquor dilution multiples were: clean water; 200; 400; 600; 800. At the same time, two spraying times of 6 hours and 36 hours before harvest and a mother liquor dilution multiple of 600 were set for comparison. The front and back sides of the tobacco leaves were sprayed evenly with a quantitative sprayer. The experiment adopted a randomized block design, with a total of 7 treatments, 3 repetitions, and a total of 21 plots, each with an area of ​​0.2 mu. After the sprayed leaves were harvested, they were baked through a conventional baking process to obtain C3F grade tobacco leaves, and aroma substances were detected and sensory evaluation was performed.

[0015] Table 1 Comparison of phenylalanine aroma substances in flue-cured tobacco leaves from the middle of Pingba

[0016]

[0017]

[0018] The results in Table 1 show that the content of phenylalanine aroma substances increased to varying degrees when the dihydrojasmonate solution of different dilutions was sprayed on the middle tobacco leaves 24 hours before harvest. The increase in benzyl alcohol content was 18.08%-41.52%, with an average of 30.54%; the increase in phenylethanol content was 8.46%-20.72%, with an average of 12.59%; the increase in benzaldehyde content was 12.94%-23.69%, with an average of 17.70%; and the increase in phenylacetaldehyde content was 27.51%-50.97%, with an average of 41.08%. The most obvious effect of increasing the content of phenylalanine aroma substances was when the tobacco leaves were sprayed 24 hours before harvest and diluted by 400-600 times.

[0019] Table 2 Comparison of sensory evaluation indexes of flue-cured tobacco leaves in the middle of Pingba

[0020] deal with Fragrance Aroma volume Miscellaneous gas Sweet fragrance Sweetness Availability 1 5.8 5.5 5.0 5.0 5.0 5.0 2 5.9 5.6 5.1 5.4 6.0 5.2 3 6.3 6.4 5.8 6.5 6.6 6.4 4 6.5 6.5 6.0 6.6 6.7 6.5 5 6.0 5.5 5.2 5.2 5.2 5.3 6 5.8 5.6 5.1 4.9 5.1 5.0 7 5.7 5.5 5.0 4.8 5.0 5.1

[0021] Note: The sensory evaluation standard for tobacco leaf quality, style and characteristics adopts a 9-scale, and Jiangsu China Tobacco and Guizhou Tobacco Science Research Institute jointly score the tobacco leaf samples.

[0022] The results in Table 2 show that when the middle tobacco leaves were sprayed with propyl dihydrojasmonate solution at different dilution ratios 24 hours before harvest, the sensory evaluation indicators were improved to varying degrees, among which the dilution ratio of 400-600 was the most obvious; the two treatments of spraying 6 hours and 36 hours before harvest had no obvious effect.

[0023] Embodiment 2:

[0024] Preparation of 5% propyl dihydrojasmonate mother solution: Prepare 52.63 ml of propyl dihydrojasmonate stock solution (purity 95%), 20 ml of anhydrous ethanol, and 4 drops of Tween, and add purified water to 1 L to obtain the mother solution, and store it in a brown bottle away from light for later use.

[0025] In 2023, in Dafang Tobacco Science and Technology Park, Bijie City, Guizhou Province, with clean water treatment as a control, 24 hours before the third kang (middle leaves) and the fifth kang (upper leaves) were harvested, the leaves to be harvested were sprayed with dihydrojasmonate solution of different dilution multiples, and 100 ml was quantitatively added to each plant. The mother liquor dilution multiples were: clean water; 200; 400; 600; 800. The front and back sides of the tobacco leaves were sprayed evenly with a quantitative sprayer. The experiment adopted a large-area experimental design, with a total of 5 treatments, each with an area of ​​0.5 mu. After the sprayed leaves were harvested, they were baked through conventional baking processes to obtain C3F grade tobacco leaves for the middle leaves and B2F grade tobacco leaves for the upper leaves, and aroma substances were detected.

[0026] Table 3 Comparison of phenylalanine aroma substances in the middle and upper parts of Dafangdian tobacco leaves after curing

[0027]

[0028] The results in Table 3 show that the content of phenylalanine aroma substances increased to varying degrees when different concentrations of propyl dihydrojasmonate solution were sprayed on the middle and upper tobacco leaves. The average increase rates of benzyl alcohol, phenylethanol, benzaldehyde and phenylacetaldehyde in the middle leaves were 28.01%, 39.32%, 47.79% and 406.69% respectively; the average increase rates of benzyl alcohol, phenylethanol, benzaldehyde and phenylacetaldehyde in the upper leaves were 30.52%, 46.875\26.695 and 90.21% respectively. For different leaf positions, the dilution multiple of 400-600 was the best.

[0029] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent of the present invention. It should be pointed out that, for ordinary technicians in this field, several variations and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A method for increasing the content of phenylalanine aroma substances in flue-cured tobacco, characterized in that: Before the tobacco leaves mature and are harvested, a propyl dihydrojasmonate solution is sprayed on the surface of the tobacco leaves. The propyl dihydrojasmonate solution is prepared by mixing a propyl dihydrojasmonate stock solution, anhydrous ethanol and Tween.

2. The method for increasing the content of phenylalanine aroma substances in flue-cured tobacco according to claim 1, characterized in that: The preparation method of the propyl dihydrojasmonate solution is as follows: 52.63 mL of the propyl dihydrojasmonate stock solution is mixed with 20 mL of anhydrous ethanol and 4 to 6 drops of Tween, and clean water is added to 1 L; when spraying, the propyl dihydrojasmonate solution is diluted 200 to 800 times with clean water.

3. The method for increasing the content of phenylalanine aroma substances in flue-cured tobacco according to claim 2, characterized in that: The tobacco leaves are middle and upper tobacco leaves, and 80 mL to 120 mL are sprayed on each tobacco leaf, with 3 to 4 tobacco leaves sprayed on each tobacco leaf.

4. The method for increasing the content of phenylalanine aroma substances in flue-cured tobacco according to claim 1, characterized in that: Spray propyl dihydrojasmonate solution 12 to 24 hours before tobacco leaves mature and are harvested.

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