Application of DA-6 in promoting growth of kidney beans in seedling stage

Abstract

The invention discloses application of DA-6 in promoting growth of kidney beans in the seedling stage, and belongs to the technical field of plant cultivation. The method comprises the following steps of: soaking seeds with DA-6 with seven different concentrations of 0 (CK), 5, 15, 25, 35, 45 and 55 mg / L by taking a Feige kidney bean variety and a local kidney bean variety of Panzhihua as research materials, and measuring indexes such as emergence rate, chlorophyll content, plant height, stem diameter, root length, root volume, root surface area, root projection area, root tip number and the like of the kidney beans. The result shows that after DA-6 seed soaking treatment, kidney bean seedling emergence is facilitated, the chlorophyll content and the plant height are increased, and meanwhile root system growth is promoted. Research results show that DA-6 seed soaking treatment can promote growth of kidney beans in the seedling stage and is most appropriate when the concentration is 45 mg / L, local kidney beans are more sensitive to DA-6, and the effect of the regulator is more remarkable.

Description

Technical Field

[0001] The invention relates to the technical field of plant cultivation, and in particular to an application of DA-6 in promoting the growth of green beans at the seedling stage. Background Art

[0002] Aminoethyl ester (DA-6) is a new type of plant cytokinin growth regulator. It has the physiological functions of increasing the content of plant chlorophyll, protein, and nucleic acid, promoting leaf photosynthesis, increasing the activity of peroxidase and nitrate reductase, promoting plant carbon and nitrogen metabolism, enhancing the absorption of water and fertilizer and the accumulation of dry matter, and improving the cold resistance of plants. It has been applied to tobacco, soybeans, cotton, fruits, vegetables, and flowers. Studies have shown that treating tobacco seeds with appropriate concentrations of DA-6 can significantly increase seedling height, stem base width, maximum leaf length, maximum leaf width, leaf area, leaf number, dry matter weight of tobacco seedling roots, stems, and leaves, root activity, and leaf chlorophyll content, and improve nitrate reductase activity. Spraying aminoethyl ester at the stage of 4 leaves and 1 heart of tobacco seedlings can promote the growth and development of tobacco seedlings, increase the root activity of tobacco seedlings, the total chlorophyll content of leaves, and the activity of nitrate reductase (NR), and reduce the activity of SOD, POD, and CAT and the content of MDA.

[0003] French beans, also known as green beans, are scientifically named Phaseolus vulgaris, and are dwarf or climbing annual herbaceous plants of the genus Phaseolus in the Fabaceae family. Among them, climbing species are generally more than 2 meters tall, with many pods, long straight and round pods, crisp and tender flesh, excellent quality and flavor, and are deeply loved by consumers. At present, a variety of regulators have been studied on French beans, but there are few studies on the effects of DA-6 on the growth and development of French beans, especially the application research on local varieties is rare in the literature. Therefore, it is very necessary to explore the effects of DA-6 on the growth of French bean seedlings, in order to provide a theoretical basis for the application of this regulator in the growth of French bean seedlings. Summary of the invention

[0004] In order to solve the above-mentioned deficiencies in the prior art, the purpose of the present invention is to provide an application of DA-6 seed soaking treatment in promoting the growth of green beans in the seedling stage, so as to provide a plant regulator that can regulate the growth of green beans in the seedling stage.

[0005] The technical solution of the present invention to solve the above technical problems is as follows: providing an application of DA-6 seed soaking treatment in promoting the growth of green beans in the seedling stage.

[0006] Further, the DA-6 concentration is 35-55 mg / L.

[0007] Furthermore, the DA-6 concentration was 45 mg / L.

[0008] Furthermore, the DA-6 seed soaking treatment is specifically as follows: soak the green bean seeds in the prepared DA-6 solution for 20-24 hours, and then place them in a glass greenhouse for cultivation.

[0009] Furthermore, the DA-6 seed soaking treatment can improve the emergence rate of green beans, which can promote the growth of green beans in the seedling stage.

[0010] Furthermore, the chlorophyll content of green beans can be increased after seed soaking treatment with DA-6, which promotes the growth of green beans in the seedling stage.

[0011] Furthermore, the growth of green beans in the seedling stage can be promoted by soaking seeds with DA-6, which can increase the plant height of green beans.

[0012] Furthermore, the DA-6 seed soaking treatment can improve the root growth of green beans, which can promote the growth of green beans in the seedling stage.

[0013] The invention has the following beneficial effects: the invention uses Feige green bean species and Panzhihua local green bean species as research materials, sets DA-6 seed soaking treatments of 0 (CK), 5, 15, 25, 35, 45 and 55 mg / L, and determines the green bean emergence rate, chlorophyll content, plant height, stem thickness, root length, root volume, root surface area, root projection area, root tip number and other indicators. The results show that DA-6 seed soaking treatment helps green bean emergence, increases chlorophyll content and plant height, and promotes root growth. The research results show that DA-6 seed soaking treatment can promote the growth of green bean seedlings, and is most suitable when the concentration is 45 mg / L, and the local green bean is more sensitive to DA-6, and the regulator effect is more significant. Therefore, the invention provides a theoretical basis for the application of the regulator in the growth of green bean seedlings. DETAILED DESCRIPTION

[0014] The following examples are only used to explain the present invention and are not intended to limit the scope of the present invention. If no specific conditions are specified in the examples, the conditions are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0015] Example 1: Effect of DA-6 Seed Soaking Treatment on Growth of French Bean Seedlings (1) Experimental materials ①Seed material: The commercial green bean seed - Fei Ge is produced by Fei Ge Agricultural Technology Co., Ltd., and the Panzhihua local variety is provided by local farmers.

[0016] ②Medicine: 8% DA-6 soluble powder, produced by Sichuan Runer Technology Co., Ltd.

[0017] ③Instruments: electronic balance (ESJ210-4B), vernier caliper (DL92150), chlorophyll meter (SPAD-502Plu), root phenotyping analysis system (analysis software is RhizoPheno), and microtek scanner (i800puls).

[0018] (2) Experimental design Different concentrations of DA-6 solution were prepared, including 5 mg / L, 15 mg / L, 25 mg / L, 35 mg / L, 45 mg / L and 55 mg / L. Each concentration was treated for 3 times, with 30 bean seeds in each treatment. Clean water was used as the control. After soaking for 24 h, the seeds were placed in seedling trays (5×10) filled with the same weight and nutrient soil and cultured in a glass greenhouse. The morphological changes of the seedlings were observed and recorded every day. The data were recorded. When the seedlings had 3 true leaves, their plant height, stem diameter, root system, chlorophyll content and other indicators were measured.

[0019] (3) Measurement indicators and methods Seedling emergence rate: number of seedlings / total number of sowing × 100%; Plant height: The height from the stem base to the plant's growing point, measured with a tape measure; Stem diameter: measured at the lower part of the first true leaf with a vernier caliper; Chlorophyll content: measured with a chlorophyll meter; Root length, root volume, root surface area, root projected area, and number of root tips: measured by root phenotyping analyzer.

[0020] (4) Data processing WPS was used to organize and plot the data, and SPSS26.0 was used for data analysis. One-way analysis of variance (ANOVA) was used for multiple comparisons between treatments to detect significant differences.

[0021] Results and Analysis (1) Effect of DA-6 seed soaking treatment on the emergence rate of green beans As shown in Table 1, DA-6 seed soaking treatment can improve the emergence rate of green beans, and different concentrations of DA-6 have different effects on the emergence rate of green beans. The emergence rate of local green beans was the largest 11 days after sowing, and gradually stabilized 13 days after sowing. The emergence rate of Feige was the largest 12 days after sowing, and gradually stabilized 14 days after sowing. After DA-6 seed soaking treatment, the emergence rate of the two varieties was the highest when treated with 45 mg / L. Compared with the control, the emergence rate of local seeds increased by 14.28%, and the emergence rate of Feige seeds increased by 14.63%. As shown in Table 1, 15 days after sowing, the emergence rate of local seeds was significantly higher than that of Feige seeds. The difference in emergence rate was the largest when treated with 15 mg / L, with a difference of 8%. In the absence of DA-6 treatment, the difference between local seeds and Feige seeds was 2%. This shows that after DA-6 seed soaking treatment, local varieties have more advantages than Feige varieties in production.

[0022] Table 1 Effects of DA-6 seed soaking treatment on the emergence rate of French beans

[0023] (2) Effects of DA-6 seed soaking on plant height and stem diameter of green beans DA-6 treatment can increase the plant height and stem diameter of green beans (see Table 2 and Table 3). For Feige, the plant height of 35 mg / L and 45 mg / L treatments on April 20 was significantly different from that of CK, increasing by 10.72% and 14.43%, respectively. On April 25, the plant height of 25 mg / L, 35 mg / L and 45 mg / L treatments was significantly different from that of CK, with the maximum increase of 28.71%. On April 30, all treatments were significantly different from CK, with the maximum increase of 80.74%. Except for the 55 mg / L treatment on April 30, there was no significant difference between the stem diameter of green beans and CK, and there were significant differences between the other times and treatments and CK. After DA-6 treatment, the effect on plant height of green beans became more obvious with the passage of time, and the stem diameter was stable between 1.56 mm and 2.78 mm from April 20 to April 30. For local varieties, there was no significant difference in plant height between each treatment and CK on April 20. On April 25, except for the 55 mg / L treatment, there was no significant difference in plant height between each treatment and CK, and the highest increase was 36.48%. On April 30, the 45 mg / L treatment was the most significantly different from CK, increasing by 91.65% compared with CK. There was a significant difference in stem diameter between each treatment and CK on April 20 and April 25. On April 30, there was a significant difference in stem diameter between each treatment and CK. After DA-6 treatment, the plant height increased more significantly than CK over time, while the stem diameter gradually stabilized.

[0024] Table 2 Effects of DA-6 seed soaking treatment on plant height and stem diameter of Philco green beans

[0025] Note: Different lowercase letters in the same column indicate significant differences (P<0.05), the same below.

[0026] Table 3 Effects of DA-6 seed soaking on plant height and stem diameter of local green beans

[0027] (3) Effect of DA-6 seed soaking treatment on chlorophyll in green bean seedlings Chlorophyll affects the photosynthetic rate, thus affecting the growth and development of plants. As can be seen from Table 4, among all the treatments, the 15 mg / L and 55 mg / L treatments had no significant difference from CK, while the other treatments had significant differences, and the chlorophyll content measured on three days was greater than that of CK. Overall, the chlorophyll content gradually increased from low concentration to high concentration, reaching the highest at 45 mg / L, which were Feige 1 (45.40), Local 1 (45.63), Feige 2 (44.33), Local 2 (45.13), Feige 3 (43.13), Local 3 (44.27), which were 33.80%, 20.71%, 21.35%, 28.94%, 16.34% and 25.41% higher than CK, respectively, and then decreased. There was no significant difference in chlorophyll content between the two varieties. The above results show that DA-6 has the most obvious increase in chlorophyll content in the two varieties at 45 mg / L, and the chlorophyll value has been stable at a high level.

[0028] Table 4 Effects of DA-6 seed soaking treatment on chlorophyll in green bean seedlings (unit: SPAD)

[0029] (4) Effect of DA-6 seed soaking treatment on root growth of green bean seedlings ① Effect of DA-6 seed soaking treatment on root growth of Philodendron chinense seedlings As shown in Table 5, the DA-6 seed soaking treatment increased the root length, root volume, root surface area, root projection area, and root tip number of Philco green beans to varying degrees. When treated with 45 mg / L, there were significant differences compared with CK on April 20, April 25, and April 30. On April 30, the root length, root volume, root surface area, root projection area, and root tip number increased most significantly compared with CK, increasing by 75.09%, 56.94%, 63.65%, 90.17%, and 133.65%, respectively.

[0030] Table 5 Effects of DA-6 seed soaking treatment on root growth of Philco green beans at the seedling stage

[0031] ② Effects of DA-6 seed soaking treatment on root growth of local green bean seedlings As shown in Table 6, on April 20, the root length, root volume, root surface area, root projection area, and root tip number of the 45 mg / L treatment were significantly different from those of CK, increasing by 45.45%, 36.52%, 45.78%, 41.75%, and 50.01%, respectively. The root volume of the 5 mg / L treatment was slightly reduced compared with that of CK. On April 25, the root length, root volume, and root projection area of ​​each treatment were significantly different from those of CK. The root length of the 45 mg / L treatment was the best, increasing by 51.37% compared with CK, the root volume of the 35 mg / L treatment was the highest, increasing by 41.10% compared with CK, and the root projection area of ​​the 5 mg / L treatment was the best, increasing by 46.37% compared with CK. The root surface area and root tip number of the 45 mg / L treatment were significantly different from those of CK, increasing by 37.53% and 97.49%, respectively, compared with CK. On April 30, except for the 55 mg / L treatment, the root length, volume, surface area, and projection area were not significantly different from those of CK, while the other treatments were significantly different from CK. Among them, the root length, volume, and surface area of ​​the 45 mg / L treatment were most significantly different from those of CK, which were 49.76%, 79.15%, and 57.63% higher than those of CK, respectively. The root projection area of ​​the 35 mg / L treatment was significantly increased compared with that of CK, and the number of root tips of the 45 mg / L treatment was significantly different from that of CK, which was 117.30% higher than that of CK. Comprehensive analysis showed that the effect of DA-6 on the root system of local green bean increased significantly over time. From April 20 to April 30, compared with the 55 mg / L DA-6 treatment, the root length, root volume, root surface area, root projection area, and root tip number of green bean seedlings after the 45 mg / L treatment began to decrease, indicating that the effect of the 45 mg / L DA-6 treatment on the root system reached the highest value, indicating that the DA-6 concentration of 45 mg / L had the best effect on the root system of green bean.

[0032] Table 6 Effects of DA-6 seed soaking treatment on root growth of local green beans at seedling stage

[0033] In summary, the results of the present invention show that DA-6 seed soaking within a certain range (35-55 mg / L) significantly increases the plant height, stem diameter, chlorophyll, root system, etc. of green beans, with 45 mg / L being the best and the concentration decreasing at 55 mg / L. The study found that compared with local varieties and Feige varieties, DA-6 seed soaking treatment has a more advantageous effect on the seedling stage of local varieties.

[0034] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. Application of DA-6 in promoting the growth of green beans in the seedling stage.

2. The use according to claim 1, characterized in that: The DA-6 concentration is 35-55 mg / L.

3. The use according to claim 2, characterized in that: The DA-6 concentration is 45 mg / L.

4. The use according to any one of claims 1 to 3, characterized in that: The DA-6 seed soaking treatment is specifically as follows: soaking green bean seeds in the prepared DA-6 solution for 20-24 hours, and then placing them in a greenhouse for cultivation.

5. The use according to claim 1, characterized in that: The method for promoting the growth of green beans in the seedling stage is to increase the emergence rate of green beans after the DA-6 seed soaking treatment.

6. The use according to claim 1, characterized in that: The method for promoting the growth of green beans in the seedling stage is to increase the chlorophyll content of green beans after the DA-6 seed soaking treatment.

7. The use according to claim 1, characterized in that: The method for promoting the growth of green beans in the seedling stage is to increase the plant height of green beans after the DA-6 seed soaking treatment.

8. The use according to claim 1, characterized in that: The method for promoting the growth of green beans at the seedling stage is that the root growth of green beans can be improved after the DA-6 seed soaking treatment.

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