Traditional Chinese medicine preparation for improving blood sugar and blood fat and application thereof

Abstract

The invention provides a traditional Chinese medicine preparation for improving blood sugar and blood fat and application thereof, and relates to the technical field of traditional Chinese medicine preparation. The traditional Chinese medicine preparation is prepared from fourteen traditional Chinese medicines including ground beetles, astragalus membranaceus, rhizoma polygonati, asparagus cochinchinensis, radix ophiopogonis, radix scrophulariae, radix trichosanthis, rhizoma atractylodis, rhizoma anemarrhenae, radix puerariae, coptis chinensis, salviae miltiorrhizae, radix paeoniae rubra and liquorice. The traditional Chinese medicine preparation can inhibit the activity of alpha-glucosidase and pancreatic lipase; typical clinical symptoms of diabetic mice can be obviously relieved, the fasting blood-glucose value of the diabetic mice can be reduced, the sugar tolerance of the mice can be enhanced, insulin secretion of the mice can be increased, and insulin resistance of the body can be relieved; the traditional Chinese medicine composition also can improve the influence of hyperlipemia on rat behavioristics, inhibit weight gain caused by high fat diet, regulate serum biochemical indexes of rats, regulate lipid metabolism, reduce accumulation of liver TC and TG caused by high fat diet, and inhibit formation of fatty liver, thereby achieving the effect of regulating blood sugar and blood fat levels. And a new treatment scheme is provided for diabetes and prevention of hyperlipemia.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine pharmaceutical preparation, and in particular to a traditional Chinese medicine preparation for improving blood sugar and blood lipid and application thereof. Background Art

[0002] In recent years, with the changes in people's lifestyles and eating habits, the prevalence of diabetes has increased rapidly and has become one of the most common diseases in the world. Diabetes is a metabolic disease characterized by absolute or relative insulin deficiency, leading to metabolic disorders of sugar, fat and protein. The typical clinical features of the disease are "three more and one less" symptoms, namely polydipsia, binge eating, polyuria and weight loss, often accompanied by symptoms such as hyperlipidemia and insulin resistance. Hyperlipidemia is a metabolic disease caused by lipid metabolism disorders, mainly manifested as more than one type of dyslipidemia, including total cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C). Hyperlipidemia is a risk factor for cardiovascular disease and the leading cause of death from cardiovascular disease worldwide. At present, diabetes in my country is facing the grim situation of high prevalence, low awareness, low control rate and high cost. Western medicine treats diabetes mainly through injection of insulin and its analogues, or oral hypoglycemic drugs such as biguanides, sulfonylureas, glinides, α-glucosidase inhibitors, thiazolidinediones, etc. The commonly used lipid-lowering drugs mainly include statins, ezetimibe, PCSK9 inhibitors and bepedic acid. Although these drugs have rapid effects, they have a single target and need to be taken for a long time, which can easily cause the body to have drug dependence and toxic side effects such as hypoglycemia, gastrointestinal reactions, and liver and kidney damage.

[0003] TCM believes that diabetes belongs to the category of "diabetes". Ancient doctors divided this disease into three types: upper, middle and lower. Most of them are caused by exogenous dampness and heat, congenital deficiency, overeating of fat, sweet and greasy food, overwork, emotional disorder, etc., which lead to lung dryness, stomach heat, kidney deficiency and triple burner disease, and finally form a disease with yin deficiency as the main symptom and excessive dryness and heat as the main symptom. The two are mutually causal and there are many variations. TCM emphasizes starting from the overall treatment of "diabetes" and its complications, and treats them based on syndrome differentiation. It also follows the principle of "prevention before illness and prevention of changes after illness". It has multi-level and multi-target action characteristics and has unique advantages in regulating the body's glucose and lipid metabolism, improving insulin resistance, and reducing toxic side effects. However, the current research on diabetes complicated with hyperlipidemia is not in-depth. Therefore, it is urgent to develop a Chinese medicine preparation that can improve blood sugar and blood lipid levels. Summary of the invention

[0004] 1. Technical issues to be resolved

[0005] In view of the deficiencies of the prior art, the present invention provides a Chinese medicine preparation for improving blood sugar and blood lipids and its application.

[0006] (II) Technical solution

[0007] To achieve the above objectives, the present invention is implemented through the following technical solutions:

[0008] A traditional Chinese medicine preparation for improving blood sugar and blood lipids, the traditional Chinese medicine preparation is composed of the following raw materials in parts by weight: 20-38 parts of astragalus, 15-34 parts of polygonatum, 22-40 parts of kudzu root, 13-30 parts of ophiopogon, 5-28 parts of scrophularia, 15-35 parts of trichosanthes, 1-13 parts of earthworm, 5-28 parts of asparagus, 5-23 parts of anemarrhena, 1-18 parts of coptis, 10-28 parts of salvia miltiorrhiza, 5-24 parts of red peony root, 1-18 parts of atractylodes, and 20-37 parts of liquorice.

[0009] Furthermore, the Chinese medicine preparation is composed of the following raw materials in parts by weight: 25-35 parts of Astragalus, 20-30 parts of Polygonatum, 25-35 parts of Pueraria, 15-25 parts of Ophiopogon, 10-20 parts of Scrophularia, 20-30 parts of Radix Trichosanthis, 5-10 parts of Eupolyphaga, 10-20 parts of Asparagus, 10-18 parts of Anemarrhena, 5-15 parts of Coptis, 15-23 parts of Salvia miltiorrhiza, 10-20 parts of Paeonia lactiflora, 5-15 parts of Atractylodes, and 25-32 parts of Licorice.

[0010] Furthermore, the Chinese medicine preparation is composed of the following raw materials in parts by weight: 30 parts of Astragalus, 25 parts of Polygonatum, 30 parts of Pueraria, 20 parts of Ophiopogon, 15 parts of Scrophularia, 25 parts of Radix Trichosanthis, 7 parts of Eupolyphaga, 15 parts of Asparagus, 13 parts of Anemarrhena, 10 parts of Coptis, 18 parts of Salvia miltiorrhiza, 15 parts of Paeonia lactiflora, 9 parts of Atractylodes, and 28 parts of Licorice.

[0011] The present invention provides a method for preparing a Chinese medicine preparation for improving blood sugar and blood lipids. The Chinese medicine preparation is prepared into any oral dosage form acceptable to the human body by conventional pharmaceutical methods and with medically acceptable auxiliary materials, including pills, powders, granules, oral liquids, decoctions, and tablets.

[0012] Furthermore, a Chinese medicine tablet for improving blood sugar and blood lipids is prepared according to the following steps:

[0013] (1) Weigh each raw material according to the above weight proportions, chop it, add 1-3 times the weight of water to soak for 30-60 minutes, filter, repeat twice, combine the filtrate, and concentrate the filtrate under reduced pressure to a relative density of 1-1.2 to obtain an extract concentrate.

[0014] (2) The extract concentrate is mixed with 1-2 times the weight of starch, dried at 60°C, and then passed through a 100-mesh sieve. 85% ethanol is used as a wetting agent to make a soft material, which is granulated with a 20-mesh sieve. After the alcohol taste is evaporated, it is dried in an oven at 60°C to obtain granules.

[0015] (3) The granules are sieved through a 14-mesh sieve, 1% magnesium stearate is added and mixed evenly, and the mixture is placed in a dry and sealed container for 24 hours. The tablet weight is calculated, and the tablet is compressed using a tablet press to obtain the Chinese medicine tablet of the present invention.

[0016] The present invention provides application of the traditional Chinese medicine preparation in preparing medicine for preventing and / or treating diabetes complicated with hyperlipidemia.

[0017] The pharmacological effects of each Chinese medicine component in the Chinese medicine preparation of the present invention are as follows:

[0018] Earth beetle: salty in taste, cold in nature, enters the liver meridian; has the effects of breaking blood, removing blood stasis, repairing tendons and bones; used for bruises, tendon injuries and fractures, blood stasis and amenorrhea, postpartum abdominal pain, and lumps.

[0019] Astragalus: sweet in taste, slightly warm in nature, enters the lung and spleen meridians; has the effects of tonifying qi and raising yang, consolidating the exterior and stopping sweating, promoting diuresis and reducing swelling, promoting fluid and nourishing blood, relieving stagnation and relieving numbness, expelling toxins and discharging pus, and astringing sores and promoting tissue regeneration; used for qi deficiency and fatigue, poor appetite and loose stools, sinking of qi in the middle, long-term diarrhea and prolapse of the anus, bloody stool and metrorrhagia, spontaneous sweating due to exterior deficiency, qi deficiency and edema, internal heat and thirst, blood deficiency and sallow complexion, hemiplegia, pain and numbness, carbuncles that are difficult to ulcerate, and long-term ulcers that do not disappear.

[0020] Polygonatum: sweet in taste, neutral in nature, enters the spleen, lung, and kidney meridians; has the effects of replenishing qi and nourishing yin, strengthening the spleen, moistening the lungs, and benefiting the kidneys; used for spleen and stomach qi deficiency, fatigue, insufficient stomach yin, dry mouth and poor appetite, dry cough due to lung deficiency, cough with blood due to fatigue, insufficient menstrual blood, soreness of the waist and knees, premature graying of hair, internal heat and thirst.

[0021] Coptis chinensis: bitter in taste, cold in nature, enters the heart, spleen, stomach, liver, gallbladder, and large intestine meridians; has the effects of clearing away heat and dampness, purging fire and detoxifying; used for damp-heat fullness, vomiting and acid regurgitation, diarrhea, jaundice, high fever and coma, hyperactivity of heart fire, restlessness and insomnia, palpitations, vomiting and bleeding due to blood heat, red eyes, toothache, diabetes, carbuncles and furuncles; external treatment of eczema, wet sores, and pus in the ear canal.

[0022] Asparagus cochinchinensis: sweet and bitter in taste, cold in nature, enters the lung and kidney meridians; has the effects of nourishing yin and moistening dryness, clearing the lungs and producing body fluid; used for dry cough due to lung dryness, sudden cough with sticky phlegm, soreness of waist and knees, bone steaming and hot flashes, internal heat and thirst, damage to body fluid due to febrile disease, dry throat and thirst, and dry intestines and constipation.

[0023] Ophiopogon japonicus: sweet, slightly bitter, slightly cold in nature, enters the heart, lung and stomach meridians; has the effects of nourishing yin, promoting body fluid, moistening the lungs and clearing the heart; used for dry cough due to lung dryness, tuberculosis due to yin deficiency, sore throat, thirst due to loss of body fluid, internal heat and thirst, restlessness and insomnia, and dry intestines and constipation.

[0024] Scrophularia: It tastes sweet, bitter and salty, is slightly cold in nature, and enters the lung, stomach and kidney meridians; it has the effects of clearing away heat and cooling blood, nourishing yin and reducing fire, detoxifying and dispersing stagnation; it is used for heat entering the blood, warm toxins causing rashes, febrile disease damaging yin, red tongue and thirst, body fluid damage and constipation, bone steaming and fatigue cough, red eyes, sore throat, diphtheria, scrofula, carbuncles and sores.

[0025] Radix Trichosanthis: It tastes sweet and slightly bitter, is slightly cold in nature, and enters the lung and stomach meridians; it has the effects of clearing away heat and purging fire, promoting fluid and quenching thirst, reducing swelling and discharging pus; it is used for thirst caused by febrile diseases, dry cough caused by lung heat, internal heat and thirst, and sores and swelling.

[0026] Atractylodes: It tastes pungent and bitter, is warm in nature, and enters the spleen, stomach, and liver meridians; it has the effects of drying dampness and strengthening the spleen, dispelling wind and cold, and improving eyesight; it is used for dampness blocking the middle burner, abdominal distension, diarrhea, edema, beriberi and paralysis, rheumatic arthralgia, colds, night blindness, and blurred vision.

[0027] Anemarrhena: bitter and sweet in taste, cold in nature, enters the lung, stomach and kidney meridians; has the effects of clearing away heat and purging fire, nourishing yin and moistening dryness; used for exogenous febrile diseases, high fever and thirst, lung heat and dry cough, bone steaming and hot flashes, internal heat and thirst, and intestinal dryness and constipation.

[0028] Pueraria root: sweet and spicy in taste, cool in nature, enters the spleen, stomach and lung meridians; has the effects of relieving muscles and reducing fever, promoting fluid production and quenching thirst, clearing rashes, raising yang and stopping diarrhea, promoting blood circulation and activating collaterals, and detoxifying alcohol; used for exogenous fever and headache, stiff neck and back pain, thirst, diabetes, measles that does not break out, heat dysentery, diarrhea, dizziness and headache, hemiplegia caused by stroke, chest pain and heart pain, and alcohol poisoning.

[0029] Salvia miltiorrhiza: bitter in taste, slightly cold in nature, enters the heart and liver meridians; has the effects of promoting blood circulation and removing blood stasis, relieving pain through menstruation, clearing the heart and relieving restlessness, cooling blood and eliminating carbuncle; used for chest pain, heart pain, abdominal and flank pain, accumulation of lumps and masses, pain due to heat, restlessness and insomnia, irregular menstruation, dysmenorrhea and amenorrhea, and sores and swelling.

[0030] Red Peony Root: bitter in taste, slightly cold in nature; enters the Liver Channel; has the effects of clearing away heat and cooling blood, dispersing blood stasis and relieving pain; used for heat entering the blood, warm toxin causing rash, vomiting blood and epistaxis, red and swollen eyes, liver depression and flank pain, amenorrhea and dysmenorrhea, abdominal pain due to lumps and masses, injuries from falls, carbuncles and ulcers.

[0031] Licorice: sweet in taste, neutral in nature, enters the heart, lung, spleen, and stomach meridians; has the effects of tonifying the spleen and replenishing qi, clearing away heat and detoxifying, removing phlegm and relieving cough, relieving pain, and harmonizing various medicines; used for spleen and stomach weakness, fatigue, palpitations and shortness of breath, cough with sputum, abdominal pain, cramps and pain in the limbs, carbuncles and sores, and relieving drug toxicity and potency.

[0032] (III) Beneficial effects

[0033] The invention provides a Chinese medicine preparation for improving blood sugar and blood lipids and its application. The Chinese medicine preparation is composed of 14 Chinese medicines including astragalus, polygonatum, kudzu root, ophiopogon, scrophularia, radix trichosanthis, earthworm, asparagus cochinchinensis, rhizoma anemarrhenae, coptis chinensis, salvia miltiorrhiza, red peony root, atractylodes macrocephala and liquorice. The earthworm is used as the main medicine in the prescription and has the effects of breaking blood and removing blood stasis, can improve blood circulation, and prevent metabolic abnormalities caused by blood stasis blocking meridians; astragalus, polygonatum and coptis chinensis are used as assistant medicines. Astragalus tonifies qi and raises yang, promotes fluid production and nourishes blood, and relieves stagnation and relieves numbness, and is an essential medicine for invigorating qi; polygonatum tonifies qi and nourishes yin, strengthens the spleen and moistens the lungs; coptis chinensis clears away heat and dries dampness, and clears away fire and detoxifies. The combination of the three can assist the main medicine in strengthening the therapeutic effects of invigorating qi and nourishing yin, clearing heat and moistening dryness, and is helpful to promote blood circulation, replenish yin fluid in the body, remove dampness and heat in the body, and improve blood sugar and blood lipids. Abnormal lipids; Asparagus, Ophiopogon, Scrophularia, Radix Trichosanthis, Atractylodes, Anemarrhena, Pueraria, Salvia miltiorrhiza, and Red Peony Root are used as adjuvants. Asparagus nourishes yin and moistens dryness, clears the lungs and produces fluid; Ophiopogon nourishes yin and produces fluid, moistens the lungs and clears the heart; Scrophularia clears heat and cools blood, nourishes yin and reduces fire; Trichosanthes clears heat and purges fire, produces fluid and quenches thirst; Atractylodes lancea dries dampness and strengthens the spleen; Anemarrhena clears heat and purges fire, nourishes yin and moistens dryness; Pueraria relieves muscles and reduces fever, produces fluid and quenches thirst; Salvia miltiorrhiza promotes blood circulation and removes blood stasis, cools blood and eliminates carbuncle; Red Peony Root clears heat and cools blood, disperses blood stasis and relieves pain. The combination of these medicines can assist the monarch and ministerial medicines to enhance the therapeutic effect, and treat symptoms such as microcirculation disorders and spleen and stomach weakness in diabetes; Licorice is used as the guiding medicine, which mainly plays the role of coordinating the various medicines and guiding the drugs directly to the diseased area. At the same time, its effect of tonifying the spleen and replenishing qi also helps to regulate the spleen and stomach functions and indirectly improve blood sugar and blood lipid metabolism.

[0034] The Chinese medicine preparation of the present invention can inhibit the activities of α-glucosidase and pancreatic lipase; significantly alleviate the typical clinical symptoms of diabetic mice, and reduce the fasting blood sugar value of diabetic mice in a dose-dependent manner, enhance the glucose tolerance of mice, and can effectively increase the secretion of insulin in diabetic mice, and alleviate the insulin resistance of the body; and can improve the influence of hyperlipidemia on the behavior of rats, effectively inhibit the weight gain caused by a high-fat diet, regulate the serum biochemical indexes of rats, regulate lipid metabolism, and can also reduce the accumulation of TC and TG in the liver caused by a high-fat diet, inhibit the formation of fatty liver, thereby achieving the effect of regulating blood sugar and blood lipid levels, and providing a new treatment plan for diabetes and prevention of diabetic complications. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 Comparison of the inhibitory activity of each group of drugs on α-glucosidase; compared with the positive drug acarbose, ** P<0.01.

[0036] Figure 2 Comparison of the inhibitory activity of each group of drugs on pancreatic lipase; compared with the positive drug oselit

[0037] ** P<0.01.

[0038] Figure 3Comparison of glucose tolerance among mice groups.

[0039] Figure 4 Comparison of serum insulin levels in each group of mice; compared with the blank control group, ## P<0.01; compared with the model control group, ** P<0.01. DETAILED DESCRIPTION

[0040] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0041] Example 1

[0042] A traditional Chinese medicine preparation for improving blood sugar and blood lipids, the traditional Chinese medicine preparation is composed of the following raw materials in parts by weight: 30 parts of astragalus, 25 parts of polygonatum, 30 parts of kudzu root, 20 parts of ophiopogon, 15 parts of scrophularia, 25 parts of trichosanthes, 7 parts of earthworm, 15 parts of asparagus, 13 parts of anemarrhena, 10 parts of coptis, 18 parts of salvia miltiorrhiza, 15 parts of red peony root, 9 parts of atractylodes, and 28 parts of liquorice.

[0043] Example 2

[0044] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is composed of the following raw materials in parts by weight: 20 parts of Astragalus, 15 parts of Polygonatum, 22 parts of Pueraria, 13 parts of Ophiopogon, 5 parts of Scrophularia, 15 parts of Radix Trichosanthis, 1 part of Eupolyphaga, 5 parts of Asparagus, 5 parts of Anemarrhena, 1 part of Coptis, 10 parts of Salvia miltiorrhiza, 5 parts of Paeonia lactiflora, 1 part of Atractylodes, and 20 parts of Licorice.

[0045] Example 3

[0046] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is composed of the following raw materials in parts by weight: 28 parts of Astragalus, 20 parts of Polygonatum, 28 parts of Pueraria, 18 parts of Ophiopogon, 10 parts of Scrophularia, 20 parts of Radix Trichosanthis, 5 parts of Eupolyphaga, 12 parts of Asparagus, 15 parts of Anemarrhena, 8 parts of Coptis, 18 parts of Salvia miltiorrhiza, 13 parts of Paeonia lactiflora, 9 parts of Atractylodes, and 28 parts of Licorice.

[0047] Example 4

[0048] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is composed of the following raw materials in parts by weight: 38 parts of Astragalus, 34 parts of Polygonatum, 40 parts of Pueraria, 30 parts of Ophiopogon, 28 parts of Scrophularia, 35 parts of Radix Trichosanthis, 13 parts of Eupolyphaga, 28 parts of Asparagus, 23 parts of Anemarrhena, 18 parts of Coptis, 28 parts of Salvia miltiorrhiza, 24 parts of Paeonia lactiflora, 18 parts of Atractylodes, and 37 parts of Licorice.

[0049] Example 5

[0050] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is composed of the following raw materials in parts by weight: 25 parts of Astragalus, 18 parts of Polygonatum, 25 parts of Pueraria, 17 parts of Ophiopogon, 8 parts of Scrophularia, 18 parts of Radix Trichosanthis, 5 parts of Eupolyphaga, 9 parts of Asparagus, 9 parts of Anemarrhena, 5 parts of Coptis, 15 parts of Salvia miltiorrhiza, 9 parts of Paeonia lactiflora, 5 parts of Atractylodes, and 25 parts of Licorice.

[0051] Example 6

[0052] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is composed of the following raw materials in parts by weight: 35 parts of Astragalus, 28 parts of Polygonatum, 32 parts of Pueraria, 26 parts of Ophiopogon, 25 parts of Scrophularia, 32 parts of Radix Trichosanthis, 10 parts of Eupolyphaga, 25 parts of Asparagus, 20 parts of Anemarrhena, 15 parts of Coptis, 25 parts of Salvia miltiorrhiza, 20 parts of Paeonia lactiflora, 17 parts of Atractylodes, and 35 parts of Licorice.

[0053] Example 7

[0054] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is composed of the following raw materials in parts by weight: 22 parts of Astragalus, 16 parts of Polygonatum, 24 parts of Pueraria, 15 parts of Ophiopogon, 7 parts of Scrophularia, 17 parts of Radix Trichosanthis, 3 parts of Eupolyphaga, 7 parts of Asparagus, 7 parts of Anemarrhena, 3 parts of Coptis, 12 parts of Salvia miltiorrhiza, 7 parts of Paeonia lactiflora, 3 parts of Atractylodes, and 22 parts of Licorice.

[0055] Example 8

[0056] The difference between this embodiment and embodiment 1 is that the Chinese medicine preparation is composed of the following raw materials in parts by weight: 32 parts of Astragalus, 25 parts of Polygonatum, 32 parts of Pueraria, 24 parts of Ophiopogon, 18 parts of Scrophularia, 28 parts of Radix Trichosanthis, 9 parts of Eupolyphaga, 18 parts of Asparagus, 18 parts of Anemarrhena, 12 parts of Coptis, 22 parts of Salvia miltiorrhiza, 18 parts of Paeonia lactiflora, 15 parts of Atractylodes, and 32 parts of Licorice.

[0057] Test Example 1

[0058] 1 Materials and methods

[0059] 1.1 Experimental drugs

[0060] Test substance 1: 30 parts of Astragalus, 25 parts of Polygonatum, 30 parts of Pueraria, 20 parts of Ophiopogon, 15 parts of Scrophularia, 25 parts of Radix Trichosanthis, 7 parts of Eupolyphaga, 15 parts of Asparagus, 13 parts of Anemarrhena, 10 parts of Coptis, 18 parts of Salvia, 15 parts of Paeonia lactiflora, 9 parts of Atractylodes, and 28 parts of Licorice.

[0061] Test substance 2: 20 parts of Ophiopogon japonicus, 15 parts of Scrophularia ningpoensis, 25 parts of Radix Trichosanthis, 7 parts of Eupolyphaga sinensis, 15 parts of Asparagus cochinchinensis, 13 parts of Anemarrhena asphodeloides, 10 parts of Coptis chinensis, 18 parts of Salvia miltiorrhiza, 15 parts of Paeonia lactiflora, 9 parts of Atractylodes macrocephala, and 28 parts of Licorice.

[0062] Test substance 3: 30 parts of Astragalus, 25 parts of Polygonatum, 30 parts of Pueraria, 7 parts of Eupolyphaga, 15 parts of Asparagus, 13 parts of Anemarrhena, 10 parts of Coptis, 18 parts of Salvia miltiorrhiza, 15 parts of Paeonia lactiflora, 9 parts of Atractylodes, and 28 parts of Licorice.

[0063] Test substance 4: 30 parts of Astragalus, 25 parts of Polygonatum, 30 parts of Pueraria, 20 parts of Ophiopogon, 15 parts of Scrophularia, 25 parts of Radix Trichosanthis, 18 parts of Salvia miltiorrhiza, 15 parts of Paeonia lactiflora, 9 parts of Atractylodes, and 28 parts of Licorice.

[0064] Test substance 5: 30 parts of Astragalus, 25 parts of Polygonatum, 30 parts of Pueraria, 20 parts of Ophiopogon, 15 parts of Scrophularia, 25 parts of Radix Trichosanthis, 7 parts of Eupolyphaga, 15 parts of Asparagus, 13 parts of Anemarrhena, and 10 parts of Coptis.

[0065] Weigh each raw material according to the above weight proportions, chop and mix each raw material, put it in a casserole, add water to soak for 10-20 minutes, boil over high heat, then simmer over low heat for 20-30 minutes, filter the decoction while it is hot; repeat twice, combine the decoctions, and concentrate to a crude drug content of 1 g / mL to obtain the Chinese medicine stock solution of test groups 1-5.

[0066] 1.2 Determination of α-glucosidase inhibition rate

[0067] The experiment set up a blank group, a control group, a sample blank group, and a sample group, namely, test groups 1-5 and an acarbose group. Samples were added sequentially in a 96-well plate according to the doses shown in Table 1 below. Three parallels were set for each group. Finally, the absorbance at 405 nm was measured using an ELISA reader, and the α-glucosidase inhibition rate was calculated according to the following formula:

[0068] α-glucosidase inhibition rate (%) = (A 样品组 -A 样品空白组 ) / (A 对照组 -A 空白组 )×100

[0069] Table 1 Amount and order of addition of each reactant (μL)

[0070]

[0071]

[0072] 1.3 Determination of pancreatic lipase inhibition rate

[0073] The experiment set up blank group, control group and sample group, namely test group 1-5 and oselit group. Take 2.5mL 0.025mol / L PBS and 1mL polyvinyl alcohol trioleylglycerol emulsion in a conical flask, mix well and place in a 37℃ constant temperature incubator for 10min, add 1mL sample, continue to react in a 37℃ incubator for 10min, add 1mL 100g / L porcine pancreatic lipase, react at 37℃ for 15min, then add 10mL 95% anhydrous ethanol, and finally add 3 drops of phenolphthalein indicator, and use 0.025mol / L NaOH to drop until slightly red. The volume of NaOH consumed by the sample group is recorded as V1; the blank group without sample and pancreatic lipase is recorded as V2; the control group without pancreatic lipase is recorded as V3; the lipase activity and pancreatic lipase inhibition rate are calculated according to the following formula:

[0074] Pancreatic lipase activity (IU) = 1000 × (V1-V2-V3) × c / t × w

[0075] Pancreatic lipase inhibition rate (%) = (1-pancreatic lipase activity in sample group / pancreatic lipase activity in control group) × 100

[0076] Wherein, c is the concentration of sodium hydroxide (mol / L); t is the reaction time after adding the enzyme (min); and w is the amount of pancreatic lipase added (g).

[0077] 1.4 Statistical methods

[0078] Graphpad Prism 8.0 statistical software was used for data processing and statistical graphs were drawn. The mean value was used for quantitative data. One-way analysis of variance was used for comparison among multiple groups, and the differences were considered statistically significant when P < 0.05.

[0079] 2 Results

[0080] 2.1α-glucosidase inhibition rate

[0081] α-Glucosidase is one of the important members in the metabolic pathways in organisms. It can regulate sugar chemical metabolism in the human body and participate in the metabolic pathways of starch and glycogen. It is also a key target for drugs to improve glucose metabolism. Figure 1The effect of each group of drugs on the activity of α-glucosidase is shown in Figure 2. Among them, the positive drug acarbose has the strongest inhibitory ability on α-glucosidase, with an inhibition rate of (60.95±4.47)%. The inhibition rate of test group 1 on α-glucosidase is (55.78±3.59)%, which is equivalent to the inhibitory effect of the positive drugs. However, due to the lack of some traditional Chinese medicine components, the inhibitory ability of test groups 2-5 was weakened to varying degrees, indicating that the components in the traditional Chinese medicine preparation of the present invention synergistically enhance the effect and none of them can be missing.

[0082] 2.2 Pancreatic lipase inhibition rate

[0083] Pancreatic lipase can break down fat into free fatty acids and monoacylglycerides. It can reduce the body's absorption of fat by inhibiting the activity of pancreatic lipase, thereby achieving the effect of lowering blood lipids. Figure 2 The effect of each group of drugs on the activity of pancreatic lipase, among which the positive drug osilistat has the strongest inhibitory effect on pancreatic lipase, with an inhibition rate of (67.03±3.01)%, and the inhibition rate of test group 1 is (65.26±2.90)%, which is equivalent to the inhibitory effect of the positive drugs. Due to the lack of some Chinese medicine components, the inhibitory ability of test groups 2-5 was weakened to varying degrees, indicating that the components in the Chinese medicine preparation of the present invention synergistically enhance the effect and jointly exert the inhibitory effect on pancreatic lipase, further verifying the scientificity and rationality of the Chinese medicine preparation of the present invention.

[0084] Test Example 2

[0085] 1 Materials and methods

[0086] 1.1 Experimental animals

[0087] 36 SPF male ICR mice, weighing 22-24 g, were provided by Beijing Weitonglihua Experimental Animal Technology Co., Ltd.

[0088] 1.2 Experimental drugs

[0089] Weigh the raw materials according to the above weight proportions: 30 parts of Astragalus, 25 parts of Polygonatum, 30 parts of Pueraria, 20 parts of Ophiopogon, 15 parts of Scrophularia, 25 parts of Radix Trichosanthis, 7 parts of Eupolyphaga, 15 parts of Asparagus, 13 parts of Rhizoma Anemarrhenae, 10 parts of Coptis, 18 parts of Salvia, 15 parts of Paeonia lactiflora, 9 parts of Atractylodes, 28 parts of Licorice. Chop the raw materials and mix them, place them in a casserole, soak them in water for 10-20 minutes, boil them over high heat, then turn to low heat and decoct them for 20-30 minutes, filter the decoction while it is hot; repeat twice, combine the decoctions, and concentrate them to a crude drug content of 1g / mL to obtain the Chinese medicine preparation of the present invention.

[0090] Metformin hydrochloride tablets, Tianjin Pacific Pharmaceutical Co., Ltd.

[0091] 1.3 Experimental animal modeling

[0092] After 36 healthy mice were adaptively fed for 3 days, 6 mice were randomly selected as blank control group, and the rest were experimental groups. The blank control group was fed with ordinary feed, and the experimental group was fed with high-fat and high-sugar feed. After 2 weeks of continuous feeding, the mice were fasted for 7 hours. The experimental group was intraperitoneally injected with streptozotocin at 100 mg / kg body weight, and the normal group was intraperitoneally injected with citric acid-sodium citrate buffer solution of the same body weight. The fasting blood glucose value was measured one week later, and the blood glucose value >11.1mmol / L was considered a successful model.

[0093] 1.4 Grouping and Dosing

[0094] The mice with successful modeling were randomly divided into five groups, namely, model control group, metformin group, high-dose Chinese medicine preparation group, medium-dose Chinese medicine preparation group, and low-dose Chinese medicine preparation group. The blank control group and the model control group were gavaged with normal saline, the metformin group was gavaged with metformin at 150 mg / kg body weight, the high-dose Chinese medicine preparation group was gavaged with the Chinese medicine preparation of the present invention at 500 mg / kg body weight, the medium-dose Chinese medicine preparation group was gavaged with the Chinese medicine preparation of the present invention at 250 mg / kg body weight, and the low-dose Chinese medicine preparation group was gavaged with the Chinese medicine preparation of the present invention at 125 mg / kg body weight, once a day, for 4 consecutive weeks.

[0095] 1.5 Detection indicators

[0096] 1.5.1 Daily indicator observation

[0097] The activity, coat glossiness, and mental state of each group of mice were observed daily, the mice's diet and water intake were recorded, and the mice's body weight and fasting blood glucose were recorded weekly.

[0098] 1.5.2 Glucose tolerance test

[0099] After the last administration, the mice were fasted for 12 h, and their fasting blood glucose was measured. Then, they were gavaged with 2 g / kg glucose solution and blood was collected from the tail vein to measure blood glucose levels at 0, 30, 60, and 120 min.

[0100] 1.5.3 Serum insulin levels

[0101] After the last administration, blood was collected from the mouse orbits and centrifuged at 4°C and 3500 rpm for 15 min to separate serum. The serum insulin level was detected according to the instructions of the ELISA kit.

[0102] 1.6 Statistical methods

[0103] Graphpad Prism 8.0 statistical software was used for data processing and statistical graphs were drawn. The mean value was used for quantitative data. ± standard deviation (s), one-way analysis of variance was used for comparison among multiple groups, and the differences were considered statistically significant when P < 0.05.

[0104] 2 Results

[0105] 2.1 Effects of various drugs on body weight and blood glucose in diabetic mice

[0106] After successful modeling, diabetic mice showed varying degrees of depression, dull body color, slow reaction, and other symptoms, accompanied by typical polyuria, polydipsia, polyphagia, and weight loss. The blank control group mice had shiny fur, good spirits, and normal reactions, and no typical symptoms of diabetes were observed. After a period of administration, compared with the blank control group, the mice in the metformin group and the Chinese medicine preparation group had a reduced water and food intake, their fur gradually regained its luster, and their mental state also recovered.

[0107] Table 1 shows the comparison of body weights of mice in each group before modeling and after drug administration. There was no significant difference in body weights of mice in each group before modeling (P>0.05); four weeks after drug administration, compared with the blank control group, the body weights of mice in the model control group were significantly reduced (P<0.01); compared with the model control group, the body weights of mice in each drug administration group were significantly increased (P<0.01), among which the increase trend of body weights of mice in the metformin group and the high-dose group of the Chinese medicine preparation was the most significant, indicating that the Chinese medicine preparation of the present invention can alleviate the symptoms of emaciation in diabetic mice and improve the typical clinical symptoms of diabetic mice.

[0108] Table 2 is a comparison of fasting blood glucose of mice in each group before modeling and after drug administration. There was no significant difference in fasting blood glucose among mice in each group before modeling (P>0.05); before drug administration, compared with the blank control group, the blood glucose levels of mice in the model control group and each drug administration group were significantly increased (P<0.01), and compared with the model control group, there was no significant difference in blood glucose levels among drug administration groups (P>0.05); after 4 weeks of drug administration, the blood glucose level of mice in the model control group was still significantly higher than that in the blank control group (P<0.01), and compared with the model control group, the fasting blood glucose levels of mice in each drug administration group were significantly reduced (P<0.01), among which the fasting blood glucose levels of mice in the metformin group and the high-dose Chinese medicine preparation group showed the most significant downward trend, indicating that the Chinese medicine preparation of the present invention can effectively reduce the blood glucose level of diabetic mice and improve the clinical symptoms of diabetic mice.

[0109] Table 1 Comparison of body weight of mice in each group

[0110] Grouping Before modeling One week Week 2 Three weeks Four weeks Blank control group 24.14±3.65 25.69±1.11 28.24±1.64 30.33±1.73 32.53±2.10 Model control group 24.25±1.84 24.20±1.24 <![CDATA[23.35±1.54 ** ]]> <![CDATA[22.32±2.23 ** ]]> <![CDATA[21.51±1.20 ** ]]> Metformin group 24.42±3.47 25.77±1.53 <![CDATA[27.20±1.51 # ]]> <![CDATA[29.33±1.04 ## ]]> <![CDATA[30.29±1.85 ## ]]> High-dose Chinese medicine preparation group 23.97±4.07 25.38±0.92 26.74±1.31 <![CDATA[28.46±0.94 ## ]]> <![CDATA[29.79±1.58 ## ]]> Chinese medicine preparation medium dose group 24.70±2.70 25.66±1.35 26.13±1.47 <![CDATA[27.54±1.24 ## ]]> <![CDATA[28.69±1.08 ## ]]> Low-dose Chinese medicine preparation group 24.20±3.48 25.62±1.11 25.52±1.25 <![CDATA[26.37±1.16 # ]]> <![CDATA[27.84±0.92 ## ]]>

[0111] Note: Compared with the blank control group, * P<0.05, ** P<0.01; compared with the model control group, # P<0.05, ## P<0.01.

[0112] Table 2 Comparison of fasting blood glucose in each group of mice

[0113] Grouping Before modeling Before administration After administration Blank control group 6.77±1.73 6.56±1.43 6.14±1.80 Model control group 6.73±1.24 <![CDATA[23.22±2.87 ** ]]> <![CDATA[23.99±2.42 ** ]]> Metformin group 6.67±2.17 <![CDATA[19.57±1.61 ** ]]> <![CDATA[7.26±0.92 ## ]]> High-dose Chinese medicine preparation group 6.02±2.30 <![CDATA[21.36±1.16 ** ]]> <![CDATA[8.31±1.21 ## ]]> Chinese medicine preparation medium dose group 5.95±1.81 <![CDATA[22.23±1.33 ** ]]> <![CDATA[12.43±1.11 ## ]]> Low-dose Chinese medicine preparation group 6.21±1.54 <![CDATA[22.98±1.58 ** ]]> <![CDATA[15.39±0.90 ## ]]>

[0114] Note: Compared with the blank control group, * P<0.05, ** P<0.01; compared with the model control group, # P<0.05, ## P<0.01.

[0115] 2.2 Effects of various drugs on glucose tolerance in diabetic mice

[0116] Figure 3 The effects of each drug on the glucose tolerance of diabetic mice were as follows: after oral administration of glucose, the blood glucose of mice reached a peak at 30 minutes, and the blood glucose of mice in the blank control group returned to normal levels at 120 minutes; compared with the blank control group, the blood glucose levels of mice in the model control group were significantly increased at each time period; compared with the model control group, the blood glucose of mice in each drug-treated group was reduced, among which the recovery of blood glucose of mice in the high-dose group of traditional Chinese medicine preparation and the positive drug metformin group was similar, indicating that the traditional Chinese medicine preparation of the present invention can improve the tolerance of diabetic mice to glucose and improve the abnormal glucose metabolism in diabetic mice.

[0117] 2.3 Effects of various drugs on serum insulin levels in diabetic mice

[0118] Figure 4 The effect of each drug on the serum insulin level of diabetic mice, compared with the blank control group, the serum insulin level of the model control group mice was significantly reduced (P < 0.01); compared with the model control group, the serum insulin level of mice in each drug group was increased, among which the serum insulin level of mice in the metformin group, the high-dose group of Chinese medicine preparation and the medium-dose group of Chinese medicine preparation was significantly increased (P < 0.01), and the serum insulin level of mice in the low-dose group of Chinese medicine preparation was not significantly different (P > 0.05), but still had an increasing trend. It shows that the Chinese medicine preparation of the present invention is beneficial to increase insulin secretion in diabetic mice and reduce insulin resistance.

[0119] 3 Conclusion

[0120] Type 2 diabetes is a common chronic disease characterized by insulin resistance and β-cell dysfunction, and the incidence rate is increasing year by year. The "three more and one less" symptoms, namely, polydipsia, polyphagia, polyuria, and weight loss, are typical clinical manifestations of diabetes. The above experimental data show that the Chinese medicine preparation of the present invention can significantly alleviate the typical clinical symptoms of diabetic mice, and can dose-dependently reduce the fasting blood sugar value of diabetic mice, enhance the glucose tolerance of mice, and effectively increase the secretion of insulin in diabetic mice, alleviate the body's insulin resistance, thereby achieving the effect of lowering blood sugar.

[0121] Test Example 3

[0122] 1 Materials and methods

[0123] 1.1 Experimental animals

[0124] Thirty-six SPF male SD rats, weighing 180-220 g, were provided by Beijing Weitonglihua Experimental Animal Technology Co., Ltd.

[0125] 1.2 Experimental drugs

[0126] Weigh the raw materials according to the above weight proportions: 30 parts of Astragalus, 25 parts of Polygonatum, 30 parts of Pueraria, 20 parts of Ophiopogon, 15 parts of Scrophularia, 25 parts of Radix Trichosanthis, 7 parts of Eupolyphaga, 15 parts of Asparagus, 13 parts of Rhizoma Anemarrhenae, 10 parts of Coptis, 18 parts of Salvia, 15 parts of Paeonia lactiflora, 9 parts of Atractylodes, 28 parts of Licorice. Chop the raw materials and mix them, place them in a casserole, soak them in water for 10-20 minutes, boil them over high heat, then turn to low heat and decoct them for 20-30 minutes, filter the decoction while it is hot; repeat twice, combine the decoctions, and concentrate them to a crude drug content of 1g / mL to obtain the Chinese medicine preparation of the present invention.

[0127] Simvastatin tablets, Shandong Luoxin Pharmaceutical Group Co., Ltd.

[0128] 1.3 Modeling, grouping and drug administration

[0129] After 1 week of adaptive feeding, the rats were randomly divided into a blank group and a high-fat model group. During the modeling period, the rats in the high-fat model group were fed with a high-fat diet, and the rats in the blank group were fed with a basic diet. During the modeling period, the rats in each group were free to drink water and the changes in body weight, water intake, food intake, and mental state were observed daily. After 5 weeks of continuous feeding, blood was collected from the tail vein of the rats in the high-fat model group to measure the levels of serum total cholesterol TC, triglycerides TG, low-density lipoprotein cholesterol LDL-C, and high-density lipoprotein cholesterol HDL-C. If any of them met the following conditions, the modeling was successful: TC>5.20mmol / L; TG>1.70mmol / L; LDL-C>3.40mmol / L; HDL-C≤1.00mmol / L.

[0130] The rats in the high-fat model group were randomly divided into a model control group, a simvastatin group, a high-dose Chinese medicine preparation group, a medium-dose Chinese medicine preparation group, and a low-dose Chinese medicine preparation group. The blank control group and the model control group were gavaged with normal saline, the simvastatin group was gavaged with 0.9 mg / kg body weight of simvastatin, the high-dose Chinese medicine preparation group was gavaged with 300 mg / kg body weight of the Chinese medicine preparation of the present invention, the medium-dose Chinese medicine preparation group was gavaged with 150 mg / kg body weight of the Chinese medicine preparation of the present invention, and the low-dose Chinese medicine preparation group was gavaged with 75 mg / kg body weight of the Chinese medicine preparation of the present invention, once a day, for 4 consecutive weeks, during which the rats ate and drank normally.

[0131] 1.4 Detection indicators

[0132] 1.4.1 Measurement of rat body weight and food intake

[0133] During the feeding period, the morphology, hair, activity, feces changes and body weight of the rats were observed, and any abnormalities were recorded in a timely manner.

[0134] 1.4.2 Serum index detection

[0135] After the last administration, rats were fasted but not watered for 12 h. After anesthesia with 7% chloral hydrate, blood was collected from the abdominal aorta and allowed to stand at room temperature for 2 h. Serum was separated after centrifugation at 4°C and 3500 rpm for 15 min. The contents of TC, TG, LDL-C, and HDL-C in serum were determined according to the instructions of the kit.

[0136] 1.4.3 Liver index and liver marker determination

[0137] After the last administration, rats were fasted but not watered for 12 h, then killed by cervical dislocation and dissected. The livers of the rats were quickly taken out and washed in precooled 0.9% saline and dried with filter paper. The organ index of each group was calculated according to the following formula after weighing. Another 0.2 g of fresh liver was taken out and made into 10% liver tissue homogenate in precooled 0.9% saline, centrifuged at 4°C and 3500 rpm for 10 min, and the supernatant was taken to detect the TC and TG contents in the rat liver according to the instructions of the kit.

[0138] Liver index (g / g) = liver weight / body weight

[0139] 1.5 Statistical methods

[0140] Graphpad Prism 8.0 statistical software was used for data processing, and the mean value was used for quantitative data. One-way analysis of variance was used for comparison among multiple groups, and the differences were considered statistically significant when P < 0.05.

[0141] 2 Results

[0142] 2.1 Effects of various drugs on behavior and body weight of hyperlipidemia rats

[0143] During the modeling period, the rats in the blank control group had normal drinking water, food intake, urine and feces, agile movements, and clean and shiny hair; the rats in the model group had reduced drinking water and food intake, darker urine color, rough feces, slow movements, mental sluggishness, and rough, messy and dull hair; after administration, the rats in each drug group gradually increased their drinking water and food intake, and their urine and feces gradually recovered; Table 3 shows the effects of each drug on the body weight and Lee's index of hyperlipidemia rats. There was no significant difference in the body weight of the rats in each group before modeling (P>0.05); before administration, compared with the blank control group, the body weight of the rats in each group was significantly increased (P<0.01), and there was no significant difference in body weight between the groups; after administration, compared with the blank control group, the body weight of the rats in the model control group was significantly increased (P<0.01); compared with the model control group, the body weight of the rats in each drug group was significantly reduced (P<0.01, P<0.05), indicating that the Chinese medicine preparation of the present invention can effectively inhibit the weight gain caused by a high-fat diet and has a certain preventive effect on obesity.

[0144] Table 3 Comparison of body weight of hyperlipidemic rats in each group

[0145] Grouping Body weight before modeling (g) Body weight before administration (g) Body weight after administration (g) Blank control group 195.26±6.47 383.53±7.52 442.39±9.90 Model control group 202.10±5.94 <![CDATA[435.21±8.57 ## ]]> <![CDATA[543.34±7.11 ## ]]> Simvastatin group 201.76±6.67 <![CDATA[432.37±12.21 ## ]]> <![CDATA[472.97±8.08 ** ]]> High-dose Chinese medicine preparation group 196.15±10.83 <![CDATA[428.01±8.16 ## ]]> <![CDATA[491.34±7.99 ** ]]> Chinese medicine preparation medium dose group 197.35±12.42 <![CDATA[432.04±12.24 ## ]]> <![CDATA[505.67±9.22 ** ]]> Low-dose Chinese medicine preparation group 205.97±9.65 <![CDATA[425.20±11.89 ## ]]> <![CDATA[520.29±5.05 * ]]>

[0146] Note: Compared with the blank control group, # P<0.05, ## P<0.01; compared with the model control group, * P<0.05, ** P<0.01.

[0147] 2.2 Effects of various drugs on serum-related parameters in hyperlipidemia rats

[0148] As shown in Table 4, compared with the blank control group, the serum TC, TG, and LDL-C levels of the rats in the model control group were significantly increased (P<0.01), and the HDL-C level was significantly decreased (P<0.01); compared with the model control group, the serum TC, TG, and LDL-C levels of the rats in the simvastatin group, the high-dose group of the Chinese medicine preparation, and the medium-dose group of the Chinese medicine preparation were significantly decreased (P<0.01, P<0.05), and the HDL-C level was significantly increased (P<0.01, P<0.05), indicating that the Chinese medicine preparation of the present invention can improve the serum biochemical indexes of rats with hyperlipidemia and regulate lipid metabolism.

[0149] Table 4 Comparison of serum related indicators of rats in each group (mol / L)

[0150] Grouping TC TG LDL-C HDL-C Blank control group 2.54±0.08 1.65±0.11 0.46±0.11 1.53±0.11 Model control group <![CDATA[3.88±0.08 ## ]]> <![CDATA[2.85±0.08 ## ]]> <![CDATA[0.93±0.10 ## ]]> <![CDATA[0.85±0.08 ## ]]> Simvastatin group <![CDATA[2.85±0.08 ** ]]> <![CDATA[1.87±0.10 ** ]]> <![CDATA[0.54±0.07 ** ]]> <![CDATA[1.33±0.10 ** ]]> High-dose Chinese medicine preparation group <![CDATA[3.08±0.14 ** ]]> <![CDATA[2.02±0.11 ** ]]> <![CDATA[0.63±0.10 ** ]]> <![CDATA[1.14±0.10 ** ]]> Chinese medicine preparation medium dose group <![CDATA[3.44±0.09 ** ]]> <![CDATA[2.32±0.09 ** ]]> <![CDATA[0.70±0.06 * ]]> <![CDATA[1.06±0.08 * ]]> Low-dose Chinese medicine preparation group 3.68±0.07 2.65±0.07 0.82±0.09 0.89±0.05

[0151] Note: Compared with the blank control group, # P<0.05, ## P<0.01; compared with the model control group, * P<0.05, **P<0.01.

[0152] 2.3 Effects of various drugs on liver index and liver parameters in hyperlipidemia rats

[0153] As shown in Table 5, compared with the blank control group, the liver index of the rats in the model control group was significantly increased (P<0.01), and the liver TC and TG levels were significantly increased (P<0.01); compared with the model control group, the liver index of the rats in each drug-treated group was significantly decreased (P<0.01), and the liver TC and TG levels of the rats in the simvastatin group, the high-dose group of the Chinese medicine preparation and the medium-dose group of the Chinese medicine preparation were significantly decreased (P<0.01). Although there was no significant difference in the liver TC and TG levels of the rats in the low-dose group of the Chinese medicine preparation, there was still a downward trend, indicating that the Chinese medicine preparation of the present invention can improve the accumulation of liver TC and TG caused by a high-fat diet, and has a certain inhibitory effect on the formation of fatty liver.

[0154] Table 5 Comparison of liver index and liver parameters of rats in each group

[0155]

[0156]

[0157] Note: Compared with the blank control group, # P<0.05, ## P<0.01; compared with the model control group, * P<0.05, ** P<0.01.

[0158] 3 Conclusion

[0159] The above experimental data show that the Chinese medicine preparation of the present invention can improve the influence of hyperlipidemia on rat behavior, effectively inhibit the weight gain caused by a high-fat diet, regulate the serum biochemical indexes of rats, regulate lipid metabolism, and can also reduce the accumulation of TC and TG in the liver caused by a high-fat diet, inhibit the formation of fatty liver, thereby achieving the effect of lowering blood lipids.

[0160] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A Chinese medicine preparation for improving blood sugar and blood lipids, characterized in that: The Chinese medicine preparation is composed of the following raw materials in parts by weight: 20-38 parts of astragalus, 15-34 parts of polygonatum, 22-40 parts of kudzu root, 13-30 parts of ophiopogon, 5-28 parts of figwort, 15-35 parts of trichosanthes, 1-13 parts of earthworm, 5-28 parts of asparagus, 5-23 parts of anemarrhena, 1-18 parts of coptis, 10-28 parts of salvia miltiorrhiza, 5-24 parts of red peony root, 1-18 parts of atractylodes, and 20-37 parts of liquorice.

2. A Chinese medicine preparation for improving blood sugar and blood lipids according to claim 1, characterized in that: The Chinese medicine preparation is composed of the following raw materials in parts by weight: 25-35 parts of astragalus, 20-30 parts of polygonatum, 25-35 parts of kudzu root, 15-25 parts of ophiopogon, 10-20 parts of scrophularia, 20-30 parts of trichosanthes, 5-10 parts of earthworm, 10-20 parts of asparagus, 10-18 parts of anemarrhena, 5-15 parts of coptis, 15-23 parts of salvia miltiorrhiza, 10-20 parts of red peony root, 5-15 parts of atractylodes, and 25-32 parts of liquorice.

3. A Chinese medicine preparation for improving blood sugar and blood lipids according to claim 1, characterized in that: The Chinese medicine preparation is composed of the following raw materials in parts by weight: 30 parts of Astragalus, 25 parts of Polygonatum, 30 parts of Pueraria, 20 parts of Ophiopogon, 15 parts of Scrophularia, 25 parts of Radix Trichosanthis, 7 parts of Eupolyphaga, 15 parts of Asparagus, 13 parts of Anemarrhena, 10 parts of Coptis, 18 parts of Salvia miltiorrhiza, 15 parts of Paeonia lactiflora, 9 parts of Atractylodes, and 28 parts of Licorice.

4. A Chinese medicine preparation for improving blood sugar and blood lipids according to any one of claims 1 to 3, characterized in that: The Chinese medicine preparation is prepared into any oral dosage form acceptable to the human body, including pills, powders, granules, oral liquids, decoctions, and tablets, by conventional pharmaceutical methods and with medically acceptable adjuvants.

5. Use of the Chinese medicine preparation according to any one of claims 1 to 3 in the preparation of a medicament for preventing and / or treating diabetes mellitus complicated with hyperlipidemia.

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