Microorganism sample processing device and detection system

Abstract

The invention relates to the technical field of microbiological detection, and discloses a microbiological sample treatment device and detection system.The treatment device comprises microbiological treatment equipment at least comprising a bacterial tablet treatment module and a plate treatment module; when the plate treatment mode is adopted, the manipulator sucks a preset amount of neutral sample from the sample container and adds the neutral sample into the plate; after the plate is diluted by the manipulator, a culture medium is injected into the plate; after the plate treatment module is subjected to capping treatment, the plate is shaken up to be stored; when the bacterial tablet processing mode is adopted, the manipulator sucks a preset amount of neutral sample from the sample container and adds the neutral sample into the bacterial tablet; and after the bacterial tablets are diluted by the manipulator, the bacterial tablet processing module automatically presses the bacterial tablets and then stores the bacterial tablets. And by arranging the microbiological treatment equipment and the manipulator, all operation procedures are automatically realized, and the actual workload of technicians is greatly reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial detection, and in particular to a microbial sample processing device and a detection system. Background Art

[0002] Microbial testing is done manually, and the process is cumbersome. It includes multiple steps such as sampling, weighing, shaking, diluting, testing, pouring culture medium, shaking, numbering, etc., all of which need to be completed manually step by step.

[0003] This will increase the workload for technical personnel, easily leading to fatigue in the standardized execution of work processes, and in turn, safety accidents will easily occur. Summary of the invention

[0004] In view of this, the present invention provides a microbial sample processing device and a detection system to solve the problems of heavy workload for technicians and prone to safety accidents.

[0005] In a first aspect, the present invention provides a microbial sample processing device, the processing device comprising:

[0006] Microorganism processing equipment, including at least a bacterial sheet processing module and a plate processing module;

[0007] A robot arm is communicatively connected with the bacterial sheet processing module and the plate processing module at the same time;

[0008] When the plate processing method is adopted, the manipulator draws a preset amount of neutral sample from the sample container and adds it to the plate; after the manipulator dilutes the plate, the culture medium is injected into the plate; after the plate processing module performs the capping process, the plate is shaken and stored;

[0009] When the bacterial slice processing method is adopted, the robot draws a preset amount of neutral sample from the sample container and adds it to the bacterial slice; after the robot dilutes the bacterial slice, the bacterial slice processing module automatically presses the bacterial slice and then stores it.

[0010] Beneficial effects: The embodiments of the present invention can automatically replace manual operation and automatically implement all operation processes by setting up microbial processing equipment and manipulators. Compared with manual detection, the detection time can be obviously optimized, and the actual workload of technicians can be greatly reduced. At the same time, since the machine equipment is controlled by a program, the consistency of each operation can be guaranteed, reducing the errors caused by manual operation. On the basis of ensuring the stability of detection, the efficiency of sequence transfer is improved, and it can be well connected with the existing information system to realize real-time interaction of data.

[0011] In an optional embodiment, the plate processing module comprises:

[0012] A plate processing module is used to open the plate filled with culture medium, register information and shake the plate;

[0013] The plate unloading module transports the shaken plates to a preset location for storage.

[0014] Beneficial effects: The embodiment of the present invention can automatically complete the processes of opening the cover, registering information, shaking and saving by setting the plate processing module and the plate unloading module, without the need for technicians to manually register and shake each plate separately, and then manually save the plate. This simplifies the operation process of the technicians and greatly reduces the actual workload of the technicians. At the same time, it also avoids human errors during registration and ensures the accuracy of information.

[0015] In an optional embodiment, the plate processing module further comprises:

[0016] The plate loading module conveys an empty plate and opens the plate cover of the empty plate; after the plate cover is opened, the robot draws a preset amount of neutral sample from the sample container and adds it into the plate.

[0017] Beneficial effects: The embodiment of the present invention can realize the function of automatically opening the plate by setting the plate loading module, without the need for technicians to open it manually, further reducing the actual workload of technicians. At the same time, after the plate loading module opens the plate cover, the robot will automatically detect the completion signal of the plate loading module, and automatically add the neutral sample to the plate, thereby improving work efficiency.

[0018] In an optional embodiment, the bacterial slice processing module includes:

[0019] The bacterial slice processing module re-covers the bacterial slice injected with the neutral sample with a bacterial slice film, and performs plate pressing and information registration processing;

[0020] The mushroom slice unloading module transports the mushroom slices that have been processed by the pressing plate and information registration to the preset position for storage.

[0021] In an optional embodiment, the bacterial slice processing module further includes:

[0022] The bacterial sheet loading module conveys an empty bacterial sheet and opens the bacterial sheet membrane of the empty bacterial sheet; after the bacterial sheet membrane is opened, the manipulator draws a preset amount of neutral sample from the sample container and adds it to the bacterial sheet.

[0023] In an optional embodiment, the microbial treatment equipment further comprises:

[0024] A PH detection module, which is communicatively connected with the manipulator;

[0025] After the sample container containing the sample is opened, the manipulator moves the sample container to the pH detection module, and the pH detection module detects the acidity and alkalinity of the sample;

[0026] The manipulator performs a neutralization operation on the sample according to the acidity and alkalinity to obtain the neutral sample.

[0027] Beneficial effect: The embodiment of the present invention can detect the actual acidity and alkalinity of the sample by setting up a pH detection module. After obtaining the test result, the robot can add neutralizing drugs to neutralize the sample according to the test result. Compared with manual detection and manual addition of drugs, the amount used during addition is more accurate, and the neutralization steps are reduced, thereby further improving work efficiency.

[0028] In an optional embodiment, the microbial treatment equipment further comprises:

[0029] The sample loading module is in communication connection with the pH detection module; the sample loading module is used to transport the sample container containing the original sample to the pH detection module.

[0030] In an optional embodiment, the microbial treatment equipment further comprises:

[0031] The cover opening module is arranged between the pH detection module and the sample loading module; when the sample loading module transports the sample container containing the original sample to the cover opening module, the cover opening module opens the sample container.

[0032] In an optional embodiment, the microbial treatment equipment further comprises:

[0033] The gradient dilution module is connected to the manipulator for communication; the gradient dilution module contains diluent, and the manipulator absorbs the diluent through a pipette and then dilutes the plate and the bacterial sheet.

[0034] In a second aspect, the present invention further provides a detection system for a microbial sample, the detection system comprising:

[0035] A microbial sample processing device as described in any one of the above embodiments;

[0036] The water bath device is suitable for storing culture medium; the manipulator is suitable for placing the stored plates and bacterial sheets into the incubator for cultivation;

[0037] The detection device is used to detect the cultured plates and bacterial slices. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the specific implementation methods of the present invention or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0039] Figure 1 A top view of a microbial sample detection system according to an embodiment of the present invention.

[0040] Description of reference numerals:

[0041] 1. Bacteria sheet loading module; 2. PH detection module; 3. Plate loading module; 4. Water bath system; 5. Robot; 6. Bacteria sheet loading module; 7. Bacteria sheet processing module; 8. Bacteria sheet unloading module; 9. Plate loading module; 10. Plate processing module; 11. Plate unloading module; 12. Gradient dilution module; 13. Conveyor belt; 14. Clean bench. DETAILED DESCRIPTION

[0042] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative work are within the scope of protection of the present invention.

[0043] In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only, and cannot be understood as indicating or implying relative importance.

[0044] In the description of the present invention, it should be noted that, unless otherwise clearly specified and limited, the terms "installed", "connected", and "connected" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection, or it can be indirectly connected through an intermediate medium, it can also be the internal communication of two components, it can be a wireless connection, or it can be a wired connection. For ordinary workers in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0045] In addition, the technical features involved in the different embodiments of the present invention described below can be combined with each other as long as they do not conflict with each other.

[0046] Microbiological testing is done manually, and the manual testing process is cumbersome. The testing process includes multiple steps such as sample collection, sample weighing, shaking, dilution, testing, culture medium pouring, shaking, numbering, etc., which need to be completed manually step by step. This makes the workload of technicians heavy, which easily leads to fatigue of the personnel in the process execution, and thus easily leads to safety accidents.

[0047] In view of this, the present invention provides a microbial sample processing device to solve the problems of heavy workload for technicians and easy occurrence of safety accidents.

[0048] Combine the following Figure 1 , describing an embodiment of the present invention.

[0049] According to an embodiment of the present invention, on the one hand, a microbial sample processing device is provided, and the processing device includes a microbial processing device and a manipulator 5 .

[0050] The microbial processing equipment is suitable for the detection of total bacterial count, coliform bacteria, total coliform bacteria, molds and yeasts, psychrophilic bacteria, aerobic spores, thermophilic aerobic spores, lactic acid bacteria, and bifidobacteria. Specifically, in an embodiment of the present invention, the microbial processing equipment at least includes a bacterial sheet processing module and a plate processing module.

[0051] In this way, the microbial treatment equipment can simultaneously perform bacterial sheet detection and national standard plate detection. Among them, bacterial sheet detection can automatically realize the steps of sample connection, cover opening, pH detection, sample suction, test tube cover opening, dilution, sample suction on bacterial sheet, pressing plate, numbering, stacking, and taking out. National standard plate detection can automatically realize the steps of sample connection, cover opening, pH detection, sample suction, plate, test tube cover opening, dilution, injection of culture medium, numbering, capping, shaking, stacking, and taking out. In this way, automated operation can optimize the previous manual detection work as a whole and improve work efficiency.

[0052] Furthermore, in the embodiment of the present invention, the robot 5 is simultaneously connected to the bacterial sheet processing module and the plate processing module in communication. That is, during the operation of the robot 5, the bacterial sheet processing module and the plate processing module can obtain the working status of the robot 5 in real time, and can make action instructions that cooperate with the robot 5 according to the working status of the robot 5, and the bacterial sheet processing module and the plate processing module can complete their respective corresponding actions according to the action instructions.

[0053] In the actual detection process, when the plate processing method is adopted, a preset amount of neutral sample can be sucked from the sample container by the manipulator 5 and added to the plate. For the loading method of the sample container, it can be selected to load automatically through the conveyor belt 13, or to load manually, or the manipulator 5 can grab the sample container to a predetermined position for loading. Of course, this embodiment is only an example of the loading process of the sample container, but it is not limited to this. Those skilled in the art can make changes according to the actual situation, as long as the same technical effect can be achieved.

[0054] At the same time, the plate processing module is provided with a cover opening device specifically used to open the plate cover. Before adding the neutral sample to the plate, the plate cover needs to be opened by the cover opening device so that the subsequent sample adding operation can be carried out.

[0055] The number of plates can be determined by those skilled in the art according to actual needs, for example, 2, 3, 4, etc. For the conveying method of the plates, the plates can be conveyed to the positions in advance by the conveyor belt 13, or the plates can be placed in sequence at the predetermined positions where the manipulator 5 works by the equipment equipped with the plates. This embodiment is only an example, but is not limited thereto, and those skilled in the art can make changes according to actual conditions as long as the same technical effect can be achieved.

[0056] Furthermore, after the neutral sample is added to the plate, a diluent can be injected into the plate by the manipulator 5 for dilution. After the plate is diluted by the manipulator 5, the culture medium is automatically injected into the plate by the manipulator 5 or the plate processing module. After the plate processing module performs the capping process, the plate is shaken for preservation. At this time, a label can be affixed to the plate after the capping process, and the relevant information of the plate is registered on the label, and then the information of the plate can be entered. For the information entry method, the technician can enter it manually, or use a barcode scanner to scan the QR code of the plate to complete the automatic entry.

[0057] Of course, this embodiment is only an example, but not a limitation thereto. Those skilled in the art may make changes according to actual conditions as long as the same technical effect can be achieved.

[0058] Similarly, similar to the plate processing method, when the bacterial slice processing method is adopted, the robot 5 draws a preset amount of neutral sample from the sample container and adds it to the bacterial slice; after the robot 5 dilutes the bacterial slice, the bacterial slice processing module automatically presses the bacterial slice and then stores it.

[0059] In this way, the embodiment of the present invention can automatically replace manual operation and automatically implement all operation processes by setting up microbial treatment equipment and manipulator 5. Compared with manual detection, the detection time can be obviously optimized, and the actual workload of technicians is greatly reduced. At the same time, since the machine equipment is controlled by a program, the consistency of each operation can be guaranteed, and the error caused by manual operation is reduced. On the basis of ensuring the stability of detection, the efficiency of sequence transfer is improved, and it can be well connected with the existing information system to realize real-time interaction of data.

[0060] Further, in an optional embodiment, the plate processing module includes a plate processing module 10 and a plate unloading module 11. Specifically, in the embodiment of the present invention, the plate processing module 10 can open the cover of the plate injected with culture medium, automatically register information, and automatically shake the plate. The plate unloading module 11 can transport the shaken plate to a preset position for storage.

[0061] In this way, the embodiment of the present invention can automatically complete the processes of opening the cover, registering information, shaking and saving by setting the plate processing module 10 and the plate unloading module 11, without the need for the technician to manually register and shake each plate separately, and then manually save the plate. This simplifies the operation process of the technician and greatly reduces the actual workload of the technician. At the same time, it also avoids human errors during registration and ensures the accuracy of the information.

[0062] Furthermore, in an optional embodiment, the plate processing module further includes a plate loading module 9. The plate loading module 9 can transport empty plates through a conveyor belt 13, and a dedicated cover opening mechanism is provided on the plate loading module 9, which can open the plate cover of the empty plate. After the plate cover is opened, the manipulator 5 draws a preset amount of neutral sample from the sample container and adds it to the plate. Specifically, two plates can be transported in parallel at the same time through the conveyor belt 13, and after the plate cover is opened, the manipulator 5 draws 2 ml of neutral sample that meets the requirements and injects 1 ml into each of the two opened plates.

[0063] In this way, the embodiment of the present invention can realize the function of automatically opening the plate by setting the plate loading module 9, without the need for the technician to open it manually, further reducing the actual workload of the technician. At the same time, after the plate loading module 9 opens the plate cover, the manipulator 5 will automatically detect the completion signal of the plate loading module 9, and automatically add the neutral sample to the plate, thereby improving work efficiency.

[0064] Further, in an optional embodiment, similar to the plate processing module, the bacterial sheet processing module includes a bacterial sheet processing module 7 and a bacterial sheet feeding module 8. Specifically, the bacterial sheet processing module 7 can re-cover the bacterial sheet injected with the neutral sample with a bacterial sheet film, and then perform a pressing plate and information registration process. The bacterial sheet feeding module 8 is used to transport the bacterial sheet after the pressing plate and information registration process to a preset position for storage.

[0065] Furthermore, in an optional embodiment, the bacterial sheet processing module also includes a bacterial sheet loading module 6, which can transport empty bacterial sheets through a conveyor belt 13. A special film opening device is provided in the bacterial sheet loading module 6, and the bacterial sheet film of the empty bacterial sheet is opened by the film opening device; after the bacterial sheet film is opened, the manipulator 5 draws a preset amount of neutral sample from the sample container and adds it to the bacterial sheet.

[0066] Furthermore, in an optional embodiment, the microbial processing equipment also includes a pH detection module 2, and the pH detection module 2 is communicatively connected to the manipulator 5. In the actual working process, the sample container can be opened by a machine device, or the sample containers can be opened manually in sequence. After the sample container containing the sample is opened, the manipulator 5 moves the sample container to the pH detection module 2, and the pH detection module 2 detects the acidity and alkalinity of the sample. The manipulator 5 performs a neutralization operation on the sample according to the acidity and alkalinity. For example, if it is an acidic sample, the manipulator 5 needs to use a pipette to absorb the medicine for adjustment. After adjustment, the sample is absorbed onto a pH test strip, and a colorimetric plate is compared by visual photography. After the comparison is within the range, the next step of detection is continued. Until the neutral sample is obtained.

[0067] With such a configuration, the embodiment of the present invention can detect the actual acidity and alkalinity of the sample by itself by setting up the pH detection module 2. After obtaining the detection result, the robot 5 can add neutralizing drugs to neutralize the sample according to the detection result. Compared with manual detection and manual addition of drugs, the amount used during addition is more accurate, and the neutralization steps are reduced, thereby further improving work efficiency.

[0068] Furthermore, in an optional embodiment, the microorganism processing device further comprises a sample loading module, which is communicatively connected to the pH detection module 2, and is used to deliver a sample container containing an original sample to the pH detection module 2. The sample loading module is specifically divided into a bacterial sheet loading module 1 and a plate loading module 3.

[0069] Furthermore, in an optional embodiment, the microorganism processing device further comprises a cover opening module, which is arranged between the pH detection module 2 and the sample loading module. When the sample loading module delivers the sample container containing the original sample to the cover opening module, the cover opening module opens the sample container.

[0070] Furthermore, in an optional embodiment, the microorganism processing equipment further includes a gradient dilution module 12, and the gradient dilution module 12 is communicatively connected to the manipulator 5. The gradient dilution module 12 stores a diluent, and the manipulator 5 draws the diluent through a pipette and then dilutes the plate and the bacterial sheet.

[0071] Furthermore, in an optional implementation, the manipulator 5 may be a mobile YUMI robot.

[0072] In a second aspect, the present invention further provides a microbial sample detection system, the detection system comprising: a microbial sample processing device, a water bath device, and a detection device as described in any one of the above embodiments.

[0073] Furthermore, the water bath device is suitable for storing culture medium, and the manipulator 5 is suitable for placing the stored plates and bacterial sheets into the incubator for culture. The detection device is used to detect the cultured plates and bacterial sheets. The detection device is well connected with the existing information system to realize data interaction and automatically issue the results.

[0074] The microbial sample detection system can be arranged in a positive pressure room, and an ultra-clean bench 14, an electronic balance, and an oscillator can also be placed in the positive pressure room.

[0075] Although the embodiments of the present invention have been described in conjunction with the accompanying drawings, those skilled in the art may make various modifications and variations without departing from the spirit and scope of the present invention, and such modifications and variations are all within the scope defined by the appended claims.

Claims

1. A microbial sample processing device, characterized in that: include: Microbial processing equipment, including at least a bacterial sheet processing module and a plate processing module; A robot (5) is connected to the bacterial sheet processing module and the plate processing module for communication; When the plate processing method is adopted, the manipulator (5) draws a preset amount of neutral sample from the sample container and adds it to the plate; after the manipulator (5) dilutes the plate, the culture medium is injected into the plate; after the plate processing module performs the capping process, the plate is shaken and stored; When the bacterial slice processing method is adopted, the robot (5) draws a preset amount of neutral sample from the sample container and adds it to the bacterial slice; after the robot (5) dilutes the bacterial slice, the bacterial slice processing module automatically presses the bacterial slice and then stores it.

2. The processing device according to claim 1, characterized in that The plate processing module comprises: A plate processing module (10) is used to open the cover of the plate injected with the culture medium, register information and shake the plate; The plate unloading module (11) transports the shaken plate to a preset position for storage.

3. The processing device according to claim 2, characterized in that The plate processing module also includes: The plate loading module (9) transports an empty plate and opens the plate cover of the empty plate; after the plate cover is opened, the robot (5) draws a preset amount of neutral sample from the sample container and adds it to the plate.

4. The processing device according to claim 1, characterized in that The bacterial slice processing module comprises: The bacterial slice processing module (7) re-covers the bacterial slice injected with the neutral sample with a bacterial slice film, and performs plate pressing and information registration processing; The mushroom slice unloading module (8) transports the mushroom slices after being pressed and processed by information registration to a preset position for storage.

5. The processing device according to claim 4, characterized in that The bacterial slice processing module also includes: The bacterial sheet loading module (6) conveys an empty bacterial sheet and opens the bacterial sheet membrane of the empty bacterial sheet; after the bacterial sheet membrane is opened, the manipulator (5) draws a preset amount of neutral sample from the sample container and adds it to the bacterial sheet.

6. The processing device according to any one of claims 1 to 5, characterized in that: The microbial treatment equipment also includes: A pH detection module (2) is communicatively connected to the manipulator (5); After the sample container containing the sample is opened, the manipulator (5) moves the sample container to the pH detection module (2), and the pH detection module (2) detects the acidity and alkalinity of the sample; The robot (5) performs a neutralization operation on the sample according to the acidity and alkalinity to obtain the neutral sample.

7. The processing device according to claim 6, characterized in that The microbial treatment equipment also includes: The sample loading module is in communication connection with the pH detection module (2); the sample loading module is used to transport the sample container containing the original sample to the pH detection module (2).

8. The processing device according to claim 7, characterized in that The microbial treatment equipment also includes: The cover opening module is arranged between the pH detection module (2) and the sample loading module; when the sample loading module transports the sample container containing the original sample to the cover opening module, the cover opening module opens the sample container.

9. The processing device according to any one of claims 1 to 5, characterized in that: The microbial treatment equipment also includes: The gradient dilution module (12) is connected to the manipulator (5) in communication; the gradient dilution module (12) stores diluent, and the manipulator (5) draws the diluent through a pipette and then dilutes the plate and the bacterial sheet.

10. A microbial sample detection system, characterized in that: include: The microbial sample processing device according to any one of claims 1 to 8; The water bath device is suitable for storing culture medium; the manipulator (5) is suitable for placing the stored plates and bacterial sheets into the incubator for culturing; The detection device is used to detect the cultured plates and bacterial slices.

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