Molecular hybridization instrument and operating device

Abstract

The invention discloses a molecular hybridization instrument and an operation device, the operation device comprises a displacement mechanism, a liquid adding and discharging mechanism and an optical detection mechanism, and the liquid adding and discharging mechanism and the optical detection mechanism are both connected with the displacement mechanism; the shifting mechanism is used for shifting the liquid adding and discharging mechanism and the optical detection mechanism to each sample storage group along the arrangement direction of the sample storage groups in the sample box; the liquid adding and discharging mechanism is used for extracting waste liquid in each sample storage position at the same time when the sample storage group is moved, or injecting a reagent into each sample storage position at the same time; and the optical detection mechanism is used for performing optical detection on each sample storage position while moving to the sample storage group. According to the operation device, the detection efficiency can be greatly improved, meanwhile, due to the fact that liquid adding and discharging and detection are conducted through the liquid adding and discharging mechanism and the optical detection mechanism respectively, structures with different use functions operate independently and do not interfere with one another, the influence of frequent action of the liquid adding and discharging mechanism on the stability of the optical detection mechanism is reduced, and stable operation of the whole machine is guaranteed.

Description

Technical Field

[0001] The present application relates to the technical field of medical equipment, and in particular to a molecular hybridization instrument and an operating device. Background Art

[0002] Nucleic acid molecular hybridization technology refers to the process in which two nucleic acid single strands (DNA or RNA) with certain homologous sequences are annealed under certain conditions according to the principle of base complementary pairing to form heteroduplexes. The current nucleic acid molecular hybridization instrument has cumbersome operating steps, low detection efficiency, and increased labor costs. Summary of the invention

[0003] The present application aims to provide a molecular hybridization instrument and an operating device, which can automatically and simultaneously perform steps such as liquid injection, waste liquid extraction, and detection on multiple sample storage locations, simplify the operating steps, and improve the operating efficiency.

[0004] According to the first aspect of the present application, the present application provides an operating device for a molecular hybridization instrument to discharge waste liquid, add reagents and perform optical detection, the operating device includes a shifting mechanism, a liquid adding and discharging mechanism and an optical detection mechanism, the liquid adding and discharging mechanism and the optical detection mechanism are both connected to the shifting mechanism, the shifting mechanism is used to shift the liquid adding and discharging mechanism and the optical detection mechanism to each sample storage group along the arrangement direction of the sample storage group in the sample box; the liquid adding and discharging mechanism is used to simultaneously extract waste liquid from each sample storage position when shifting to the sample storage group, or, simultaneously add reagents to each sample storage position; the optical detection mechanism is used to simultaneously perform optical detection on each sample storage position when shifting to the sample storage group.

[0005] In one embodiment, the liquid adding and discharging mechanism includes a liquid adding and discharging drive assembly and a plurality of liquid adding and discharging groups, wherein the plurality of liquid adding and discharging groups are installed at the power output end of the liquid adding and discharging drive assembly, and the plurality of liquid adding and discharging groups correspond one by one to each sample storage position in the sample storage group; the liquid adding and discharging drive assembly is used to drive the plurality of liquid adding and discharging groups to move toward or away from each sample storage position in the sample storage group when shifting to the sample storage group; the liquid adding and discharging group includes a liquid discharging needle and a liquid injection needle, wherein the liquid discharging needle is used to extract waste liquid from the sample storage position, and the liquid injection needle is used to inject reagents into the sample storage position.

[0006] In one embodiment, the liquid adding and discharging mechanism also includes a liquid adding and discharging limiting unit, which is electrically connected to the liquid adding and discharging driving assembly and is used to reset the liquid adding and discharging driving assembly to the initial position of liquid adding and discharging when the liquid adding and discharging driving assembly drives the multiple liquid adding and discharging groups to move in a direction away from the sample storage position, so that the liquid adding and discharging groups avoid the sample storage position.

[0007] In one embodiment, the length of the injection needle is shorter than the length of the drainage needle.

[0008] In one embodiment, the optical detection mechanism includes an optical detection drive assembly and a plurality of optical detection modules, wherein the plurality of optical detection modules are arranged along the direction in which the sample storage positions in the sample storage group are arranged, and the plurality of optical detection modules are connected to a power output end of the optical detection drive assembly, wherein the optical detection drive assembly is used to drive the plurality of optical detection modules to move toward or away from the sample storage positions when shifting to the sample storage group, and the optical detection modules are used to perform optical detection on the sample storage positions when moving toward the sample storage positions.

[0009] In one embodiment, the optical detection mechanism further includes an optical detection limit unit, which is electrically connected to the optical detection drive assembly and is used to reset the optical detection module to an initial detection position when the optical detection drive assembly drives the optical detection module to move away from the sample storage position.

[0010] In one embodiment, the optical detection mechanism further includes a light source module, which is installed at the power output end of the optical detection drive assembly and fixedly connected to a plurality of the optical detection modules, and is used to emit an illumination light source to the sample storage position.

[0011] In one embodiment, the shift mechanism includes a shift drive power source and a shift transmission assembly, the shift drive power source is connected to the shift transmission assembly, the liquid adding and discharging mechanism and the optical detection mechanism are both fixed to the shift transmission assembly, and the shift drive power source is used to drive the shift transmission assembly output to shift along the arrangement direction of the sample storage group in the sample box, and shift to each sample storage group.

[0012] In one embodiment, the shift mechanism further includes an adjusting unit, which is electrically connected to the shift driving power source, and is used to adjust the displacement output by the shift driving power source to drive the shift transmission assembly.

[0013] According to the second aspect of the present application, the present application provides a molecular hybridization instrument, comprising the operating device described above; and also comprising a hybridization platform and a swing drive mechanism, wherein the sample box is mounted on the hybridization platform, and the swing drive mechanism is transmission-connected to the hybridization platform, and the swing drive mechanism is used to drive the hybridization platform to tilt to a preset angle when extracting liquid from the sample storage position, or to drive the hybridization platform to be in a horizontal state when injecting liquid into the sample storage position.

[0014] According to the molecular hybridization instrument and operating device of the above-mentioned embodiment, under the action of the shifting mechanism, the liquid adding and discharging mechanism can simultaneously extract waste liquid or simultaneously add reagents to each sample storage position in the sample storage group, and the optical detection mechanism can simultaneously perform optical detection on each sample storage position in the sample storage group, which can greatly improve the detection efficiency. At the same time, since liquid adding and discharging and detection are respectively performed by the liquid adding and discharging mechanism and the optical detection mechanism, the structures with different use functions operate independently without interfering with each other, reducing the impact of the frequent operation of the liquid adding and discharging mechanism on the stability of the optical detection mechanism, and ensuring the stable operation of the whole machine. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 A schematic diagram of a sample box provided in the related art Figure 1 ;

[0016] Figure 2 A schematic diagram of a sample box provided in the related art Figure 2 ;

[0017] Figure 3 The three-dimensional operating device provided by this application Figure 1 ;

[0018] Figure 4 The three-dimensional operating device provided by this application Figure 2 ;

[0019] Figure 5 A schematic diagram of a liquid injection and discharge assembly in an operating device provided in the present application;

[0020] Figure 6 A three-dimensional diagram of a liquid discharge drive assembly and an optical detection drive assembly in the operating device provided by the present application;

[0021] Figure 7 A partial structural stereogram of a liquid discharge drive assembly and an optical detection drive assembly in the operating device provided by the present application;

[0022] Figure 8 An exploded view of a portion of the structure of a liquid discharge drive assembly and an optical detection drive assembly in the operating device provided in the present application;

[0023] Fig. 9 A three-dimensional diagram of an optical detection module in the operating device provided by the present application;

[0024] Fig.10 An exploded view of the optical detection module in the operating device provided by the present application;

[0025] Fig.11 A top view of the operation device provided in the present application cooperating with the sample box to discharge and inject liquid;

[0026] Fig.12 A front view of the operating device provided in the present application cooperating with the sample box to discharge and inject liquid;

[0027] Fig.13 for Fig.12 Cross-section view in the AA direction;

[0028] Fig.14 A top view of the operating device provided in the present application and the sample box cooperating to take photos;

[0029] Fig.15 A front view of the operation device provided in this application cooperating with the sample box to take a photo;

[0030] Fig.16 for Fig.15 Cross-sectional view along the BB direction.

[0031] Reference numerals:

[0032] A shift mechanism 10, a shift driving power source 11, a shift transmission assembly 12, a shift bracket 121, a cantilever 1211, a driving wheel 122, a driven wheel 123, and a transmission belt 124;

[0033] Add a discharge mechanism 20, add a discharge drive assembly 21, add a discharge drive power source 211, add a discharge bracket 212, add a discharge sliding portion 213, add a discharge sliding member 214, add a discharge rack 2141, add a discharge coupling 215, add a discharge gear 216, add a discharge group 22, a discharge needle 221, a discharge outlet 2211, an injection needle 222, an injection inlet 2221, a needle holder 223, a discharge limit unit 23, a discharge photoelectric switch 231, and a discharge limit block 232;

[0034] Optical detection mechanism 30, optical detection drive assembly 31, optical detection drive power source 311, optical detection bracket 312, optical detection sliding part 313, optical detection sliding member 314, optical detection rack 3141, optical detection coupling 315, optical detection gear 316, optical detection fixing block 317, optical detection module 32, optical detection circuit board 321, optical detection light shield 322, optical detection limit unit 33, optical detection photoelectric switch 331, optical detection limit block 332, light source module 34, diffuser 341, light shield 342;

[0035] Sample box 100, sample storage group 101, sample storage position 102;

[0036] Hybridization platform 200;

[0037] Swing drive mechanism 300. DETAILED DESCRIPTION

[0038] The present application is further described in detail below by specific embodiments in conjunction with the accompanying drawings. Wherein similar elements in different embodiments adopt associated similar element numbers. In the following embodiments, many detailed descriptions are intended to enable the present application to be better understood. However, those skilled in the art can easily recognize that some of the features can be omitted in different situations, or can be replaced by other elements, materials, and methods. In some cases, some operations related to the present application are not shown or described in the specification, in order to avoid the core part of the present application being overwhelmed by too much description, and for those skilled in the art, it is not necessary to describe these related operations in detail, and they can fully understand the related operations based on the description in the specification and the general technical knowledge in the art.

[0039] In addition, the features, operations or characteristics described in the specification can be combined in any appropriate manner to form various implementations, and the operation steps involved in each embodiment can also be replaced or adjusted in order in a manner that is obvious to those skilled in the art. Therefore, the specification and drawings are only for the purpose of clearly describing a certain embodiment, and do not mean that the composition and / or order are necessary.

[0040] The serial numbers of the components in this document, such as "first", "second", etc., are only used to distinguish the objects described and do not have any order or technical meaning. The "connection" and "coupling" mentioned in this application, unless otherwise specified, include direct and indirect connections (couplings).

[0041] See also Figure 1 and Figure 2 As shown, the sample box 100 is usually carried on the hybridization platform 200 of the molecular hybridization instrument. The sample box 100 is provided with multiple rows of sample storage groups 101, each row of sample storage groups 101 is arranged in parallel along the length direction of the sample box 100, and each row of sample storage groups 101 is provided with multiple sample storage positions 102, wherein the positions of the sample storage positions 102 of two adjacent rows of sample storage groups 101 correspond to each other. In the related art, it can be formed as follows Figure 1 The sample box 100 with 16 rows of sample storage groups 101 and 3 sample storage positions 102 in each row can also be formed as shown in FIG. Figure 2 The sample box 100 shown in FIG. 1 has 24 rows of sample storage groups 101, and each row has 4 sample storage positions 102. Thus, a sample box 100 having 48 throughputs (eg, Figure 1 ) and 96 throughput (as shown Figure 2 ) Sample box 100 with two throughput formats.

[0042] However, a molecular hybridization instrument usually only uses one liquid adding needle and one liquid discharging needle to add reagents to each sample box and discharge waste liquid. Therefore, it is necessary to operate the sample storage positions 102 in the sample box 100 one by one, and after the operation is completed, an optical detection step is performed, and the optical detection mechanism also performs detection one by one. This operation method has cumbersome operation steps, low detection efficiency, and increased labor costs.

[0043] In response to the above problems, the present application provides a molecular hybridization instrument and an operating device, which can simultaneously perform the steps of extracting waste liquid, adding reagents, or optically detecting each sample storage position 102 in each row of sample storage group 101, and each step is completed automatically, which can significantly improve the detection efficiency and reduce labor costs.

[0044] Among them, the molecular hybridization instrument uses molecular hybridization technology to detect whether the gene to be tested contains inhibitory gene sequences. Specifically, DNA molecules usually have a stable double-stranded helical structure. When there are denaturing factors, the hydrogen bonds inside the DNA molecules are broken and dissociated into two single-stranded DNAs. After removing the denaturing factors, the single-stranded DNA can be combined into a stable double-stranded helical structure through base complementarity. This process is called the renaturation process. During the renaturation process, if there is an exogenous single-stranded DNA fragment or oligonucleotide that is identical or highly homologous to a certain part of the sample nucleic acid sequence, the two can form a hybrid (i.e., a double-stranded DNA molecule) through complementary bases. This process is called hybridization. If a known DNA fragment is added to the renatured DNA and can be combined into a double-stranded molecule, it means that the sample contains a known DNA sequence.

[0045] One of the samples involved in hybridization must be labeled in order to generate a hybridization signal that can be detected by optical detection. The labeled sample is called a labeled sample. For example, it can be added to the sample storage position 102 through one of the reagent filling steps, and the sample hybridized with the labeled sample is called a tested sample. For example, the labeled sample is a nucleic acid molecule of a known specific sequence and carries a marker that can be detected optically.

[0046] In the following embodiments, the sample storage position 102 in each sample storage group 101 of the sample box 100 can be a slot-shaped structure set in the sample box 100, and the sample to be detected is stored inside. The sample to be detected is the sample to be detected. The sample to be detected is usually fixed on a solid support, and the labeled sample is free in the solution. Commonly used solid supports include nitrocellulose filter membranes, nylon membranes, etc., and the solid support is set at the bottom of the slot of the sample storage position 102.

[0047] The operating device provided in the present application is applied to a molecular hybridization instrument, and can discharge waste liquid in the sample storage position 102 , or add reagents to the sample storage position 102 , or perform optical detection on the sample in the sample storage position 102 .

[0048] See also Figure 3 As shown, the operating device includes a shifting mechanism 10, a liquid adding and discharging mechanism 20 and an optical detection mechanism 30. The liquid adding and discharging mechanism 20 and the optical detection mechanism 30 are both connected to the shifting mechanism 10. The shifting mechanism 10 is used to shift the liquid adding and discharging mechanism 20 and the optical detection mechanism 30 to each sample storage group 101 along the arrangement direction of the sample storage group 101 in the sample box 100, as shown in FIG. Figure 1 As shown, each sample storage group 101 in the sample box 100 is arranged along the length direction of the sample box 100 , and the shifting mechanism 10 is equivalent to outputting a displacement along the length direction of the sample box 100 to shift the liquid adding and discharging mechanism 20 and the optical detection mechanism 30 to each sample storage group 101 .

[0049] When the liquid adding and discharging mechanism 20 is shifted to one of the sample storage groups 101 by the shifting mechanism 10 , the liquid adding and discharging mechanism 20 simultaneously extracts waste liquid from each sample storage position 102 of the sample storage group 101 , or simultaneously injects reagents into each sample storage position 102 of the sample storage group 101 .

[0050] Among them, the waste liquid in the sample storage position 102 is the reagents left over from the previous detection process, so when there is a previous detection step, it should be extracted through the liquid filling and discharge mechanism 20, and when the previous detection step is not performed, only the reagents need to be added to the sample storage position 102.

[0051] It can be understood that when the shifting mechanism 10 is in operation, the liquid adding and discharging mechanism 20 and the optical detection mechanism 30 are shifted to the first sample storage group 101, and the waste liquid is extracted from each sample storage position 102 in the sample storage group 101 at the same time through the liquid adding and discharging mechanism 20, or, reagents are added to each sample storage position 102 in the sample storage group 101 at the same time, and so on, until the waste liquid extraction and reagent addition operations are completed for the last sample storage group 101.

[0052] Of course, when there is waste liquid left over from the previous detection step, after the displacement mechanism 10 is displaced to each sample storage group 101, the liquid adding and discharging mechanism 20 can be used to simultaneously extract waste liquid from each sample storage position 102. After the waste liquid is extracted, the displacement mechanism 10 moves in the opposite direction of the movement when the waste liquid is extracted, and after being displaced to each sample storage group 101, the reagent is simultaneously added to each sample storage position 102 through the liquid adding and discharging mechanism 20.

[0053] It should be noted that, for filling a plurality of reagents into the sample storage position 102 , it is necessary to repeat a plurality of steps of filling the reagents.

[0054] For example, when the shifting mechanism 10 is shifted to the sample storage group 101, and there is waste liquid left over from the previous detection step, the liquid adding and discharging mechanism 20 simultaneously extracts waste liquid from each sample storage position 102, and then adds different types of reagents to each sample storage position 102 in multiple times. Of course, after all sample storage positions 102 are extracted, the reagent adding step is performed only once each time the sample storage positions 102 are shifted, and this is repeated until all reagents are filled.

[0055] If there is no waste liquid left over from the previous detection step in the sample storage position 102, then only the step of adding reagents is required.

[0056] Among them, different reagents added to the sample in the sample storage position 102 can make the sample to be tested release single-stranded DNA, and renature with the single-stranded DNA labeled sample homologous to it in one of the reagents, so as to obtain double-stranded DNA molecules after renaturation hybridization, which can indicate that the sample contains a known DNA sequence.

[0057] For the double-stranded DNA molecules after hybridization, since there is a marker in the single-stranded DNA of the labeled sample, the marker can be detected by the optical detection mechanism 30 to determine whether the sample to be detected contains a known DNA sequence. Specifically, the optical detection mechanism 30 is used to perform optical detection on each sample storage position 102 at the same time when it is shifted to the sample storage group 101. In other words, the optical detection mechanism 30 can perform optical detection on each sample storage position 102 in the same sample storage group 101 each time.

[0058] Thus, in the operating device for molecular hybridization instrument provided by the present application, under the action of the shifting mechanism 10, the liquid adding and discharging mechanism 20 can simultaneously extract waste liquid or simultaneously add reagents to each sample storage position 102 in the sample storage group 101, and the optical detection mechanism 30 can simultaneously perform optical detection on each sample storage position 102 in the sample storage group 101, so that the detection efficiency can be greatly improved. At the same time, since liquid adding and discharging and detection are respectively performed by the liquid adding and discharging mechanism 20 and the optical detection mechanism 30, the structures with different use functions operate independently without interfering with each other, reducing the influence of the frequent operation of the liquid adding and discharging mechanism 20 on the stability of the optical detection mechanism 30, and ensuring the stable operation of the whole machine.

[0059] like Figure 3 As shown, the shifting direction of the shifting mechanism 10 is the X-axis direction, and the X-axis direction corresponds to the length direction of the sample box 100, that is, the liquid adding and discharging mechanism 20 and the optical detection mechanism 30 are shifted above the sample box 100, and the liquid adding and discharging mechanism 20 and the optical detection mechanism 30 need to move toward or away from the sample box 100, and the direction is Figure 3The Z-axis direction shown in the figure is used to move closer to or farther away from the sample box 100, so as to facilitate operations such as extracting waste liquid, adding reagents, and optical detection. Please refer to the subsequent embodiments for details.

[0060] See also Figure 3-Figure 5 As shown, the liquid adding and discharging mechanism 20 includes a liquid adding and discharging driving component 21 and a plurality of liquid adding and discharging groups 22. The plurality of liquid adding and discharging groups 22 are installed at the power output end of the liquid adding and discharging driving component 21. The plurality of liquid adding and discharging groups 22 correspond one by one to each sample storage position 102 in the sample storage group 101. The liquid adding and discharging driving component 21 is used to drive the plurality of liquid adding and discharging groups 22 to move toward or away from each sample storage position 102 in the sample storage group 101 when shifting to the sample storage group 101.

[0061] The liquid adding and discharging group 22 includes a liquid discharging needle 221 and a liquid injection needle 222. In other words, each sample storage position 102 corresponds to a liquid discharging needle 221 and a liquid injection needle 222. The liquid discharging needle 221 is used to extract waste liquid in the sample storage position 102 after the liquid adding and discharging drive component 21 drives it to move toward the sample storage position 102. The liquid injection needle 222 is used to inject reagent into the sample storage position 102 after the liquid adding and discharging drive component 21 drives it to move toward the sample storage position 102.

[0062] It should be noted that if Figure 5 As shown, the discharge needle 221 and the injection needle 222 are both mounted on a needle holder 223, which is fixed to the power output end of the liquid addition and discharge drive assembly 21, wherein the discharge needle 221 has a discharge outlet 2211, and the injection needle 222 has an injection inlet 2221, and the discharge outlet 2211 can be connected to a waste liquid collection device through a negative pressure adsorption structure to collect the extracted waste liquid, while the injection inlet 2221 is connected to a reagent source through an injection pump to inject the reagent into the sample storage position 102 through the injection needle 222. The reagent source can switch different types of reagents so that the injection pump pumps different types of reagents.

[0063] As mentioned above, the sample storage position 102 is a slot-type structure, so when the liquid addition and discharge driving component 21 drives the liquid addition and discharge group 22 to move toward the sample storage position 102, it is necessary to at least extend the liquid discharge needle 221 to the bottom of the slot of the sample storage position 102 or to have a small gap with the bottom of the slot, so as to completely extract the waste liquid. The liquid addition and discharge driving component 21 drives the liquid addition and discharge group 22 to move away from the sample storage position 102, so that the liquid discharge needle 221 and the liquid injection needle 222 are reset to a position away from the sample storage position 102, thereby avoiding the sample storage position 102 at the last position for the next shift.

[0064] The length of the injection needle 222 is shorter than that of the discharge needle 221 , so that the discharge needle 221 can at least extend to the bottom of the sample storage position 102 or have a small gap with the bottom of the sample storage position 102 each time.

[0065] After the steps of pumping waste liquid and adding reagents are completed, the liquid adding and draining group 22 needs to be reset. Figure 7 and Figure 8 As shown, the liquid adding and discharging mechanism 20 also includes a liquid adding and discharging limiting unit 23, which is electrically connected to the liquid adding and discharging driving assembly 21. The liquid adding and discharging limiting unit 23 is used to reset to the liquid adding and discharging initial position when the liquid adding and discharging driving assembly 21 drives the multiple liquid adding and discharging groups 21 to move in a direction away from the sample storage position 102, so that the liquid adding and discharging groups 22 avoid the sample storage position 102.

[0066] It should be noted that the initial position for adding and discharging liquid is the initial position where the adding and discharging liquid group 22 is not driven by the adding and discharging liquid driving assembly 21 to move toward the sample storage position 102. In the process where the adding and discharging liquid mechanism 20 and the optical detection mechanism 30 are driven by the shifting mechanism 10 to shift along the arrangement direction of the sample storage group 101 in the sample box 100 at the initial position for adding and discharging liquid, the adding and discharging liquid group 22 can avoid the sample storage position 102.

[0067] See also Figure 11-13 As shown, after the operating device is applied to the molecular hybridization instrument, a swing drive mechanism 300 is further provided below the hybridization platform 200. The swing drive mechanism 300 is in transmission connection with the hybridization platform 200 and is used to drive the hybridization platform 200 to tilt at a preset angle relative to the horizontal plane when the liquid discharge needle 221 draws liquid from the sample storage position 102, so that the sample storage position 102 tilts at a preset angle, and the liquid discharge needle 221 extends to the bottom of the tilted sample storage position 102. The tilted hybridization platform 200 can concentrate the waste liquid at the lowest point of the sample storage position 102, so as to completely draw the waste liquid. When the step of drawing the waste liquid is completed and the sample storage position 102 needs to be filled with reagents, the swing drive mechanism 300 drives the hybridization platform 200 to be in a horizontal state.

[0068] It should be noted that, since the discharge needle 221 needs to extend to the bottom of the groove of the sample storage position 102 or have a small gap with the bottom of the groove when moving toward the sample storage position 102, at this time, the sample storage position 102 is in a tilted state, then when the waste liquid extraction step is completed and the reagent needs to be added to the sample storage position 102, the sample storage position 102 is in a horizontal state under the action of the swing drive mechanism 300. In the above process, the liquid addition and discharge drive assembly 21 should drive the liquid addition and discharge group 22 to move a preset distance away from the sample storage position, so as to at least allow the discharge needle 221 to avoid the sample storage position 102 that switches from the tilted state to the horizontal state. The preset distance can ensure that the discharge needle 221 and the injection needle 222 are in the inner cavity of the sample storage position 102, so that the injection needle 222 can add reagents to the sample storage position 102.

[0069] Of course, the preset moving distance may also be the distance that enables the liquid adding and discharging group 22 to move to the initial position of liquid adding and discharging, and it is only necessary to ensure that the liquid adding needle 222 can add reagents to the sample storage position 102 .

[0070] In this embodiment, the liquid addition and discharge limit unit 23 includes a liquid addition and discharge photoelectric switch 231 and a liquid addition and discharge limit block 232. The liquid addition and discharge photoelectric switch 231 is electrically connected to the liquid addition and discharge drive component 21. The liquid addition and discharge photoelectric switch 231 can be used to emit photoelectric signals or receive reflected photoelectric signals, and the liquid addition and discharge limit block 232 is used to reflect the photoelectric signals emitted by the liquid addition and discharge photoelectric switch 231 by reflection, so as to control the liquid addition and discharge drive component 21 to stop working after the liquid addition and discharge photoelectric switch 231 receives the reflected photoelectric signal, so as to ensure that the liquid addition and discharge group 22 moves to the liquid addition and discharge initial position in the direction away from the sample storage position 102.

[0071] The position where the liquid addition and discharge limit block 232 is set is to enable the liquid addition and discharge group 22 to be located at the initial liquid addition and discharge position after moving in the direction away from the sample storage position 102. In this position, the liquid addition and discharge limit block 232 can reflect the photoelectric signal emitted by the liquid addition and discharge photoelectric switch 231 by reflection.

[0072] Continue to see Figure 6-Figure 8As shown, the liquid addition and discharge driving assembly 21 includes a liquid addition and discharge driving power source 211, a liquid addition and discharge bracket 212, a liquid addition and discharge sliding portion 213 and a liquid addition and discharge sliding member 214. The liquid addition and discharge bracket 212 is installed at the power output end of the displacement mechanism 10, the liquid addition and discharge sliding portion 213 is fixed to the liquid addition and discharge bracket 212, and the liquid addition and discharge sliding member 214 is slidably installed on the liquid addition and discharge sliding portion 213, and can output a sliding motion toward or away from the sample box 100. Multiple liquid discharge needles 221 and multiple liquid injection needles 222 are all connected to the liquid addition and discharge sliding member 214. In this embodiment, the needle holders 223 of the multiple liquid discharge needles 221 and the multiple liquid injection needles 222 are fixedly connected to the liquid addition and discharge fixed block 217, and the liquid addition and discharge fixed block 217 is connected to the liquid addition and discharge sliding member 214.

[0073] The liquid adding and discharging driving power source 211 is fixed to the liquid adding and discharging bracket 212 and is transmission-connected to the liquid adding and discharging sliding member 214. The liquid adding and discharging driving power source 211 is used to drive the liquid adding and discharging sliding member 214 to slide back and forth along the liquid adding and discharging sliding portion 213 to drive the liquid discharging needle 221 and the liquid injection needle 222 to move toward or away from the sample storage position 102.

[0074] In a specific embodiment, the liquid adding and discharging sliding member 214 is a liquid adding and discharging rack 2141, the liquid adding and discharging driving power source 211 is connected to the liquid adding and discharging gear 216 through the liquid adding and discharging coupling 215, and the liquid adding and discharging gear 216 is meshed with the liquid adding and discharging rack 2141, and the liquid adding and discharging driving power source 211 can drive the liquid adding and discharging gear 216 to rotate through the liquid adding and discharging coupling 215, so that the liquid adding and discharging rack 2141 converts the rotational motion of the liquid adding and discharging gear 216 into linear motion.

[0075] The sliding direction and sliding distance of the liquid filling and discharge sliding member 214 can be adjusted by setting the direction and torque output by the liquid filling and discharge driving power source 211 .

[0076] See also Figure 3 , Figure 4 , Fig. 9 and Fig.10 As shown, the optical detection mechanism 30 includes an optical detection drive component 31 and a plurality of optical detection modules 32. The plurality of optical detection modules 32 are arranged along the direction in which the sample storage positions 102 in the sample storage group 101 are arranged, and the plurality of optical detection modules 32 are all connected to the power output end of the optical detection drive component 31. The optical detection drive component 31 is used to drive the plurality of optical detection modules 32 to move toward or away from the sample storage position 102 when the optical detection mechanism 30 is shifted to the sample storage group 101, and the optical detection module 32 is used to perform optical detection on the sample storage position 102 when moving toward the sample storage position 102.

[0077] Specifically, the optical detection module 32 is a camera that can capture the marking information on the hybridization membrane strip in the sample storage location 102 to determine whether the single-stranded DNA of the marked sample and the single-stranded DNA of the sample to be tested form a hybridized double-stranded DNA molecule.

[0078] See also Fig. 9 and Fig.10 As shown, multiple optical detection modules 32 are mounted on an optical detection circuit board 321, and the optical detection circuit board 321 is fixed on an optical detection light shield 322, and the optical detection modules 32 pass through the optical detection light shield 322 and extend to the inside of the optical detection light shield 322, and an optical detection cover 321 connected to the optical detection light shield 322 is also covered and buckled above the optical detection circuit board 321. The optical detection light shield 322 is connected to the power output end of the optical detection driving component 31 to indirectly drive the multiple optical detection modules 32 to move synchronously toward or away from the sample storage position 102.

[0079] See also Figure 7 and Figure 8 As shown, the optical detection mechanism 30 also includes an optical detection limit unit 33, which is electrically connected to the optical detection drive assembly 31 and is used to reset the optical detection module 32 to the detection initial position when the optical detection drive assembly 31 drives the optical detection module 32 to move away from the sample storage position 102.

[0080] It should be noted that the detection initial position is the initial position where the optical detection module 32 is not driven by the optical detection driving assembly 31 to move toward the sample storage position 102. In the process of driving the liquid adding and discharging mechanism 20 and the optical detection mechanism 30 to shift along the arrangement direction of the sample storage group 101 in the sample box 100 through the shifting mechanism 10 at the detection initial position, the optical detection module 32 can avoid the sample storage position 102.

[0081] See also Figure 8 As shown, the optical detection limit unit 33 includes an optical detection photoelectric switch 331 and an optical detection limit block 332. The optical detection photoelectric switch 331 is electrically connected to the optical detection drive component 31. The optical detection photoelectric switch 331 can be used to emit a photoelectric signal or receive a reflected photoelectric signal, and the photoelectric detection limit block 332 is used to reflect the photoelectric signal emitted by the optical detection photoelectric switch 331 by reflection, so as to control the optical detection drive component 31 to stop working after the optical detection photoelectric switch 331 receives the reflected photoelectric signal, so as to ensure that the optical detection module 32 moves to the detection initial position in the direction away from the sample storage position 102.

[0082] The optical detection limit block 332 is set at a position that enables the optical limit module 32 to be in the initial detection position after moving in a direction away from the sample storage position 102. In this position, the photoelectric detection limit block 332 can reflect the photoelectric signal emitted by the optical detection photoelectric switch 331 by reflection.

[0083] See also Figure 6-Figure 8 As shown, the optical detection drive assembly 31 includes an optical detection drive power source 311, an optical detection bracket 312, an optical detection sliding portion 313 and an optical detection sliding member 314. The optical detection bracket 312 is installed at the power output end of the shift mechanism 10, the optical detection sliding portion 313 is fixed to the optical detection bracket 312, and the optical detection sliding member 314 is slidably installed on the optical detection sliding portion 313, and can output a sliding motion toward or away from the sample storage position 102. A plurality of optical detection modules 32 are connected to the optical detection sliding member 314. In this embodiment, the plurality of optical detection modules 32 are fixedly connected to the optical detection fixed block 317, and the optical detection fixed block 317 is connected to the optical detection sliding member 314.

[0084] The optical detection driving power source 311 is fixed to the optical detection bracket 312 and is in transmission connection with the optical detection sliding member 314. The optical detection driving power source 311 is used to drive the optical detection sliding member 314 to slide back and forth along the optical detection sliding portion 313 to drive the optical detection module 32 to move toward or away from the sample storage position 102.

[0085] In a specific embodiment, the optical detection sliding member 314 is an optical detection rack 3141, the optical detection driving power source 311 is connected to the optical detection gear 316 through the optical detection coupling 315, and the optical detection gear 316 is meshed with the optical detection rack 3141, the optical detection driving power source 311 can drive the optical detection gear 316 to rotate through the optical detection coupling 315, so that the optical detection rack 3141 converts the rotational motion of the optical detection gear 316 into linear motion.

[0086] The sliding direction and sliding distance of the optical detection slider 314 can be adjusted by setting the direction and torque output by the optical detection driving power source 311 .

[0087] like Fig. 9 and Fig.10 As shown, in order to improve the detection effect of the optical detection module 32, the optical detection mechanism 30 also includes a light source module 34, which is installed at the power output end of the optical detection drive component 31 and is fixedly connected to multiple optical detection modules 32. The light source module 34 is used to emit an illumination light source to the sample storage position 102.

[0088] In a specific embodiment, the light source module 34 is a light panel, which is installed on the inclined side of the shading cover 322. In order to achieve a better lighting effect, a diffuser 341 is provided on the side of the light source module 34 facing the shading cover 322, and a shading plate 342 is provided on the side of the light source module 34 facing away from the diffuser 341.

[0089] See also Figure 3 and Figure 4 As shown, the shift mechanism 10 includes a shift drive power source 11 and a shift transmission assembly 12. The shift drive power source 11 is connected to the shift transmission assembly 12. The liquid adding and discharging mechanism 20 and the optical detection mechanism 30 are both fixed to the shift transmission assembly 12. The shift drive power source 11 is used to drive the output of the shift transmission assembly 12 to shift along the arrangement direction of the sample storage group 101 in the sample box 100, and shift to each sample storage group 101.

[0090] In a specific embodiment, the shift transmission assembly 12 includes a shift bracket 121, a driving wheel 122, a driven wheel 123 and a transmission belt 124. The driving wheel 122 and the driven wheel 123 are spaced apart from each other and rotatably installed on the shift bracket 121. The shift drive power source 11 is fixed to the shift bracket 121 and is transmission-connected to the driving wheel 122. The transmission belt 124 is sleeved on the driving wheel 122 and the driven wheel 123. The shift drive power source 11 is used to drive the driving wheel 122 to rotate, thereby driving the driven wheel 123 to rotate through the transmission belt 124, and the liquid adding and discharging mechanism 20 and the optical detection mechanism 30 are both fixedly connected to the transmission belt 124, so as to drive the liquid adding and discharging mechanism 20 and the optical detection mechanism 30 to move simultaneously when the transmission belt 124 moves.

[0091] The direction and displacement of the liquid adding and discharging mechanism 20 and the optical detection mechanism 30 can be adjusted by setting the direction and torque output by the displacement driving power source 10 .

[0092] In this embodiment, the displacement bracket 121 has a cantilever 1211 , and the driving wheel 122 and the driven wheel 123 are both mutually detected and rotatably mounted on the cantilever 1211 , and the cantilever 1211 is located above the sample box 100 .

[0093] In one embodiment, the spacing between adjacent sample storage groups 101 in different flux sample boxes 100 is different, so the distance that the shift mechanism 10 moves each time corresponding to different flux sample boxes 100 is also different. Therefore, the shift mechanism 10 provided in the present application also includes an adjustment unit, which is electrically connected to the shift drive power source 11. The adjustment unit is used to adjust the displacement output by the shift drive power source 11 to drive the shift transmission assembly 12, that is, to adjust the steering and torque output by the shift drive power source 11 for adjustment.

[0094] See also Figure 11-Figure 16As shown, the present application also provides a molecular hybridization instrument, including the operating device in the above embodiment. It also includes a hybridization platform 200 and a swing drive mechanism 300, the sample box 100 is installed on the hybridization platform 200, the swing drive mechanism 300 is connected to the hybridization platform 200 by transmission, and the swing drive mechanism 300 is used to drive the hybridization platform 200 to tilt at a preset angle when the sample storage position 102 is pumped, or to drive the hybridization platform 200 to be in a horizontal state when the sample storage position 102 is injected.

[0095] In summary, in the molecular hybridization instrument and operating device provided by the present application, under the action of the shifting mechanism, the liquid adding and discharging mechanism can simultaneously extract waste liquid or simultaneously add reagents to each sample storage position in the sample storage group, and the optical detection mechanism can simultaneously perform optical detection on each sample storage position in the sample storage group, which can greatly improve the detection efficiency. At the same time, since liquid adding and discharging and detection are respectively performed by the liquid adding and discharging mechanism and the optical detection mechanism, the structures with different use functions operate independently without interfering with each other, reducing the impact of the frequent operation of the liquid adding and discharging mechanism on the stability of the optical detection mechanism, and ensuring the stable operation of the whole machine.

[0096] The above specific examples are used to illustrate the present invention, which is only used to help understand the present invention and is not intended to limit the present invention. For those skilled in the art, according to the concept of the present invention, some simple deductions, modifications or substitutions can be made.

Claims

1. An operating device for a molecular hybridization instrument for discharging waste liquid, adding reagents and optical detection, characterized in that: The operating device includes a shifting mechanism, a liquid adding and discharging mechanism and an optical detection mechanism, wherein the liquid adding and discharging mechanism and the optical detection mechanism are both connected to the shifting mechanism, and the shifting mechanism is used to shift the liquid adding and discharging mechanism and the optical detection mechanism to each sample storage group along the arrangement direction of the sample storage groups in the sample box; the liquid adding and discharging mechanism is used to extract waste liquid from each sample storage position at the same time when shifting to the sample storage group, or to add reagents to each sample storage position at the same time; the optical detection mechanism is used to perform optical detection on each sample storage position at the same time when shifting to the sample storage group.

2. The operating device according to claim 1, characterized in that The liquid adding and discharging mechanism includes a liquid adding and discharging driving assembly and a plurality of liquid adding and discharging groups, wherein the plurality of liquid adding and discharging groups are installed at the power output end of the liquid adding and discharging driving assembly, and the plurality of liquid adding and discharging groups correspond one by one to each sample storage position in the sample storage group; the liquid adding and discharging driving assembly is used to drive the plurality of liquid adding and discharging groups to move toward or away from each sample storage position in the sample storage group when shifting to the sample storage group; the liquid adding and discharging group includes a liquid discharging needle and a liquid injection needle, wherein the liquid discharging needle is used to extract waste liquid from the sample storage position, and the liquid injection needle is used to inject reagent into the sample storage position.

3. The operating device according to claim 2, characterized in that The liquid adding and discharging mechanism also includes a liquid adding and discharging limiting unit, which is electrically connected to the liquid adding and discharging driving assembly and is used to reset to the liquid adding and discharging initial position when the liquid adding and discharging driving assembly drives the multiple liquid adding and discharging groups to move in a direction away from the sample storage position, so that the liquid adding and discharging groups avoid the sample storage position.

4. The operating device according to claim 2, characterized in that The length of the injection needle is shorter than the length of the discharge needle.

5. The operating device according to claim 1, characterized in that: The optical detection mechanism includes an optical detection drive component and a plurality of optical detection modules. The plurality of optical detection modules are arranged along the direction in which the sample storage positions in the sample storage group are arranged, and the plurality of optical detection modules are connected to the power output end of the optical detection drive component. The optical detection drive component is used to drive the plurality of optical detection modules to move toward or away from the sample storage positions when shifting to the sample storage group, and the optical detection modules are used to perform optical detection on the sample storage positions when moving toward the sample storage positions.

6. The operating device according to claim 5, characterized in that The optical detection mechanism further comprises an optical detection limit unit, which is electrically connected to the optical detection drive assembly and is used for resetting to a detection initial position when the optical detection drive assembly drives the optical detection module to move in a direction away from the sample storage position.

7. The operating device according to claim 5, characterized in that The optical detection mechanism also includes a light source module, which is installed at the power output end of the optical detection drive assembly and fixedly connected to a plurality of the optical detection modules. The light source module is used to emit an illumination light source to the sample storage position.

8. The operating device according to claim 1, characterized in that: The shift mechanism includes a shift drive power source and a shift transmission assembly, the shift drive power source is connected to the shift transmission assembly, the liquid adding and discharging mechanism and the optical detection mechanism are both fixed to the shift transmission assembly, and the shift drive power source is used to drive the shift transmission assembly output to shift along the arrangement direction of the sample storage groups in the sample box and shift to each sample storage group.

9. The operating device according to claim 8, characterized in that The shift mechanism further comprises an adjusting unit, which is electrically connected to the shift driving power source and is used to adjust the displacement output by the shift driving power source driving the shift transmission assembly.

10. A molecular hybridization instrument, characterized in that: It comprises an operating device as described in any one of claims 1 to 9; and also comprises a hybridization platform and a swing drive mechanism, wherein the sample box is mounted on the hybridization platform, the swing drive mechanism is transmission-connected to the hybridization platform, and the swing drive mechanism is used to drive the hybridization platform to tilt to a preset angle when extracting liquid from a sample storage position, or to drive the hybridization platform to be in a horizontal state when injecting liquid into the sample storage position.

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