Method for determining concentration of coenzyme Q10 in biological matrix

Abstract

The invention relates to a method for determining the concentration of coenzyme Q10 in a biological matrix, which comprises the following steps: mixing a peripheral blood sample with a diluent, then adding an oxidizing agent to carry out oxidation reaction, after the oxidation reaction is finished, adding an alcohol solvent into the reaction liquid of the oxidation reaction to carry out protein precipitation reaction, and extracting coenzyme Q10, a suspension with the absorbance of 2.5-3.5 is obtained, the suspension is subjected to centrifugal treatment, and supernate is obtained; determining the concentration of the coenzyme Q10 in the supernate by using a liquid chromatography-mass spectrometry technology; converting the concentration of the coenzyme Q10 in the supernatant into the concentration of the coenzyme Q10 in the peripheral blood sample; performing linear fitting on the concentration of the coenzyme Q10 in multiple groups of existing peripheral blood and the concentration of the coenzyme Q10 in the biological matrix to obtain a related equation; and obtaining the concentration of the coenzyme Q10 in the biological matrix according to the concentration of the coenzyme Q10 in the peripheral blood sample and the associated equation. According to the method, the concentration of the coenzyme Q10 in the biological matrix can be detected only by 2 microliters of peripheral blood sample at least.

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