Donkey-hide gelatin with effect of improving senile dementia and preparation method of donkey-hide gelatin

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to donkey-hide gelatin with an effect of improving senile dementia and a preparation method thereof. The donkey-hide gelatin with the effect of improving senile dementia is prepared from the following raw materials in parts by mass: 15 to 30 parts of donkey-hide gelatin, 10 to 20 parts of radix angelicae sinensis, 12 to 22 parts of rhizoma atractylodis macrocephalae, 5 to 15 parts of herba rhodiolae, 1 to 10 parts of folium ginkgo, 7 to 17 parts of radix codonopsis and 1 to 14 parts of fructus lycii. The colla corii asini with the effect of improving senile dementia, prepared by the invention, is clear and simple in formula and good in compounding effect, and has a very good curative effect on senile dementia.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to donkey-hide gelatin with the effect of improving senile dementia and a preparation method thereof. Background Art

[0002] Dementia, usually referred to as Alzheimer's disease (AD), is a neurodegenerative disease that mainly affects the cognitive function of the elderly. It leads to the gradual decline of memory, thinking ability and behavioral ability, seriously affecting the quality of life of patients and bringing a heavy burden to families and society.

[0003] Although the Western medicines currently used to treat Alzheimer's disease (especially Alzheimer's disease) have some effects in relieving symptoms and delaying disease progression, they also have some disadvantages and limitations. Commonly used drugs: Donepezil, Galantamine, Rivastigmine, common side effects include nausea, vomiting, diarrhea, loss of appetite, etc. It may cause bradycardia or syncope, especially in the elderly.

[0004] Traditional Chinese medicine in my country has the characteristics of focusing on comprehensive and holistic conditioning; distinguishing both syndromes and constitutions; correcting diseases and curing diseases, with a wide range of applications; and mild, stable and long-lasting medicinal effects. However, some Chinese medicine formulas achieve therapeutic effects by adding a large number of effective ingredients, and fail to clarify the synergistic effects and mechanisms of Chinese medicine raw materials and active ingredients. Instead, the intake of too many drugs has toxic side effects on the body.

[0005] Therefore, there is an urgent need for a donkey-hide gelatin having the effect of improving Alzheimer's disease and a preparation method thereof. Summary of the invention

[0006] The purpose of the present invention is to provide donkey-hide gelatin with the effect of improving senile dementia and a preparation method thereof.

[0007] In order to achieve the above object, the present invention provides the following technical solutions:

[0008] A donkey-hide gelatin with the effect of improving Alzheimer's disease comprises the following raw materials in parts by weight: 15-30 parts of donkey-hide gelatin, 10-20 parts of angelica, 12-22 parts of atractylodes, 5-15 parts of rhodiola, 1-10 parts of ginkgo leaves, 7-17 parts of codonopsis and 1-14 parts of wolfberry.

[0009] Furthermore, the following raw materials are included in parts by weight: 15-20 parts of donkey-hide gelatin, 10-15 parts of angelica, 17-22 parts of atractylodes, 5-10 parts of rhodiola, 1-5 parts of ginkgo leaves, 8-12 parts of codonopsis and 9-14 parts of wolfberry.

[0010] Furthermore, the following raw materials are included in parts by weight: 17 parts of donkey-hide gelatin, 12 parts of angelica, 20 parts of atractylodes, 7 parts of rhodiola, 3 parts of ginkgo leaves, 11 parts of codonopsis and 9 parts of wolfberry.

[0011] The present invention provides a method for preparing donkey-hide gelatin having the effect of improving senile dementia, comprising the following steps:

[0012] (1) mixing angelica sinensis, atractylodes macrocephala, and rhodiola rosea and crushing them to less than 300 mesh to obtain a mixture A, mixing the mixture A with water in a weight ratio of 1:(1-10), adjusting the pH to 8.5-8.7, and preparing a mixed solution A;

[0013] (2) adding alkaline protease to the mixed solution A, and performing enzymolysis at 45°C to 48°C for 40 to 60 minutes; inactivating the enzyme, adjusting the pH value to neutral, adding neutral protease, and performing enzymolysis at 45°C to 48°C for 60 to 70 minutes; inactivating the enzyme, and continuously adding ficin, and performing enzymolysis at 45°C to 48°C for 50 to 60 minutes; inactivating the enzyme to obtain an enzymolysis solution; taking the supernatant after centrifugation, and freeze-drying the solution to obtain powder A;

[0014] (3) Ginkgo biloba leaves, Codonopsis pilosula and Lycium barbarum are mixed and crushed to less than 300 meshes to obtain a mixture B, and the mixture B is mixed with water in a weight ratio of 1: (1 to 10) to obtain a mixed solution B;

[0015] (4) adding the composite bacteria to the mixed solution B, fermenting at 30-35° C. for 15-20 h, sterilizing, and obtaining a fermentation solution; centrifuging the fermentation solution, taking the supernatant, and freeze-drying the solution to obtain a powder B;

[0016] (5) After the donkey-hide gelatin is crushed, it is soaked in rice wine and stewed in water until it melts. Powder A and powder B are added, mixed evenly, and concentrated into a thick paste with a relative density of 1.10 to 1.13. After the thick paste is cooled, donkey-hide gelatin with the effect of improving Alzheimer's disease is obtained.

[0017] The present invention selects Chinese medicinal materials with clear pharmacological effects by selecting selected raw materials, making the composition concise, and reasonably matching different medicinal materials to utilize the synergistic effect between them to enhance the overall therapeutic effect. According to the effective ingredient content and pharmacological effect of each medicinal material, the best ratio is determined to ensure that the contribution of each medicinal material in the combination is maximized. By accurately controlling the dosage of each medicinal material, the side effects that may be caused by excessive use of certain ingredients are avoided.

[0018] Furthermore, the dosage of the alkaline protease is 600-800u / mL of the mixed solution A; the dosage of the neutral protease is 1000-1200u / mL of the mixed solution A; and the dosage of the ficin is 800-900u / mL of the mixed solution A.

[0019] Furthermore, the composite bacteria include Lactobacillus casei, Bifidobacterium bifidum and Bifidobacterium longum.

[0020] Further, the dosage of Lactobacillus casei is 10 9 ~10 10 CFU / mL mixed solution B; the dosage of Bifidobacterium bifidum is 10 7 ~10 8 CFU / mL mixed solution B; the dosage of Bifidobacterium longum is 10 8 ~10 9 CFU / mL mixed solution B.

[0021] The present invention is carried out by enzymolysis of angelica, atractylodes macrocephala, rhodiola rosea respectively, and fermentation of ginkgo leaf, codonopsis pilosula and wolfberry, and the prepared donkey-hide gelatin with the effect of improving Alzheimer's disease can be more effective for Alzheimer's disease. Angelica, atractylodes macrocephala, rhodiola rosea are enzymolyzed, and the activity produced by fermentation of ginkgo leaf, codonopsis pilosula and wolfberry is released during the enzymolysis process, and new active substances are produced by fermentation. By enzymolysis of angelica, atractylodes macrocephala, rhodiola rosea respectively, fermentation of ginkgo leaf, codonopsis pilosula and wolfberry, and then combined with donkey-hide gelatin, this composite preparation can play the effect of improving Alzheimer's disease from multiple angles. It not only improves the bioavailability and activity of each component, but also acts together through multiple mechanisms to achieve the effect of comprehensive conditioning.

[0022] Furthermore, a 5-7 wt % NaOH solution is used to adjust the pH to 8.5-8.7.

[0023] Furthermore, 7-9 wt % citric acid is used to adjust the pH value to neutral.

[0024] Furthermore, after the donkey-hide gelatin is crushed, 5 to 8 times the mass of rice wine as the donkey-hide gelatin is added and soaked for 12 to 15 hours, and then stewed in water until melted.

[0025] Compared with the prior art, the advantages and beneficial effects of the present invention are:

[0026] 1. The present invention selects Chinese medicinal materials with clear pharmacological effects by selecting selected raw materials and concise composition. By rationally matching different medicinal materials and utilizing the synergistic effect between them, the overall therapeutic effect is enhanced. According to the effective ingredient content and pharmacological effect of each medicinal material, the optimal ratio is determined to ensure that the contribution of each medicinal material in the combination is maximized. By accurately controlling the dosage of each medicinal material, the side effects that may be caused by excessive use of certain ingredients are avoided.

[0027] 2. The present invention prepares donkey-hide gelatin with the effect of improving Alzheimer's disease by enzymolysis of angelica, atractylodes macrocephala and rhodiola rosea respectively, and fermentation of ginkgo leaf, codonopsis pilosula and wolfberry, which can be more effective in treating Alzheimer's disease. DETAILED DESCRIPTION

[0028] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Lactobacillus brevis, number: CICC 24627, was purchased from China Industrial Microbiological Culture Collection Center.

[0030] Lactobacillus plantarum, number: CI CC 25125, was purchased from China Industrial Microbiological Culture Collection Center.

[0031] Bifidobacterium animalis, number: CICC 24932, was purchased from China Industrial Microbiological Culture Collection Center.

[0032] Lactobacillus casei, number: CICC 25035, was purchased from China Industrial Microbiological Culture Collection Center.

[0033] Bifidobacterium bifidum, number: CICC 6168, was purchased from China Industrial Microbiological Culture Collection Center.

[0034] Bifidobacterium longum, number: CI CC 25033, was purchased from China Industrial Microbiological Culture Collection Administration Center.

[0035] The enzymes used in the present invention were purchased from Shanghai Yuanye Biotechnology Co., Ltd. Alkaline protease, item number S10154, 200 U / mg Neutral protease, item number S10013, 100 U / mg.

[0036] Example 1

[0037] The present embodiment provides a donkey-hide gelatin with the effect of improving Alzheimer's disease, comprising the following raw materials in parts by weight: 17 parts of donkey-hide gelatin, 12 parts of angelica sinensis, 20 parts of atractylodes macrocephala, 7 parts of rhodiola rosea, 3 parts of ginkgo leaves, 11 parts of codonopsis pilosula and 9 parts of wolfberry.

[0038] The method for preparing donkey-hide gelatin having the effect of improving Alzheimer's disease comprises the following steps:

[0039] (1) mixing angelica sinensis, atractylodes macrocephala, and rhodiola rosea and crushing them to less than 300 mesh to obtain a mixture A, mixing the mixture A with water at a weight ratio of 1:8, and adjusting the pH to 8.6 with a 6 wt % NaOH solution to obtain a mixed solution A;

[0040] (2) adding alkaline protease to the mixed solution A, and performing enzymatic hydrolysis at 46° C. for 50 min; inactivating the enzyme, adjusting the pH value to neutral using 8 wt % citric acid, adding neutral protease, and performing enzymatic hydrolysis at 46° C. for 65 min; inactivating the enzyme, continuing to add fig protease, and performing enzymatic hydrolysis at 46° C. for 55 min;

[0041] The amount of alkaline protease used is 700u / mL mixed solution A; the amount of neutral protease used is 1100u / mL mixed solution A; the amount of ficin used is 850u / mL mixed solution A. The enzymes are inactivated to obtain an enzymatic solution; after centrifugation, the supernatant is taken and freeze-dried to obtain powder A;

[0042] (3) Ginkgo biloba leaves, Codonopsis pilosula and Lycium barbarum are mixed and crushed to less than 300 meshes to obtain a mixture B, and the mixture B is mixed with water in a weight ratio of 1:8 to obtain a mixed solution B;

[0043] (4) adding the composite bacteria to the mixed solution B, fermenting at 32° C. for 17 h, and sterilizing to obtain a fermentation solution; the fermentation solution was centrifuged, the supernatant was collected, and freeze-dried to obtain powder B;

[0044] The composite bacteria include Lactobacillus casei, Bifidobacterium bifidum and Bifidobacterium longum. The dosage of Lactobacillus casei is 10 9 CFU / mL mixed solution B; the dosage of Bifidobacterium bifidum is 10 7 CFU / mL mixed solution B; the dosage of Bifidobacterium longum is 10 8 CFU / mL mixed solution B.

[0045] (5) After the donkey-hide gelatin is crushed, it is added with rice wine of 6 times the mass of the donkey-hide gelatin and soaked for 14 hours, and then stewed in water until it melts. Powder A and powder B are added, mixed evenly, and concentrated into a thick paste with a relative density of 1.12. After the thick paste is cooled, donkey-hide gelatin with the effect of improving Alzheimer's disease is obtained.

[0046] Example 2

[0047] The difference between this embodiment and embodiment 1 is that the present embodiment comprises the following raw materials in parts by weight: 15 parts of donkey-hide gelatin, 10 parts of angelica sinensis, 17 parts of atractylodes macrocephala, 5 parts of rhodiola rosea, 1 part of ginkgo leaf, 8 parts of codonopsis pilosula and 9 parts of wolfberry.

[0048] Example 3

[0049] The difference between this embodiment and embodiment 1 is that the present embodiment comprises the following raw materials in parts by weight: 20 parts of donkey-hide gelatin, 15 parts of angelica sinensis, 22 parts of atractylodes macrocephala, 10 parts of rhodiola rosea, 5 parts of ginkgo leaves, 12 parts of codonopsis pilosula and 14 parts of wolfberry.

[0050] Example 4

[0051] The difference between this embodiment and embodiment 1 is that the present embodiment comprises the following raw materials in parts by weight: 15 parts of donkey-hide gelatin, 20 parts of angelica sinensis, 12 parts of atractylodes macrocephala, 15 parts of rhodiola rosea, 1 part of ginkgo leaf, 17 parts of codonopsis pilosula and 1 part of wolfberry.

[0052] Example 5

[0053] The present embodiment is different from the embodiment 1 in that the present embodiment comprises the following raw materials in parts by weight: 30 parts of donkey-hide gelatin, 10 parts of angelica sinensis, 22 parts of atractylodes macrocephala, 5 parts of rhodiola rosea, 10 parts of ginkgo leaves, 7 parts of codonopsis pilosula and 14 parts of wolfberry.

[0054] Comparative Example 1

[0055] The difference between this comparative example and Example 1 is: a donkey-hide gelatin with the effect of improving Alzheimer's disease, comprising the following raw materials in parts by weight: 17 parts of donkey-hide gelatin, 4 parts of angelica, 7 parts of atractylodes, 17 parts of rhodiola, 16 parts of ginkgo leaves, 2 parts of codonopsis and 19 parts of wolfberry.

[0056] Comparative Example 2

[0057] The difference between this comparative example and Example 1 is that Angelica sinensis, Atractylodes macrocephala and Rhodiola rosea are not subjected to enzymatic hydrolysis.

[0058] The Chinese angelica, white atractylodes and rhodiola rosea were crushed into pieces smaller than 300 meshes and soaked in water 10 times the weight of the raw materials for 12 hours. After soaking, the raw materials were decocted with water for three times, the decoctions were combined, concentrated, and freeze-dried to obtain powder A.

[0059] Comparative Example 3

[0060] The difference between this comparative example and Example 1 is that the ginkgo leaves, codonopsis pilosula and wolfberry are not fermented.

[0061] Ginkgo leaves, Codonopsis pilosula and Lycium barbarum were crushed into pieces smaller than 300 meshes and soaked in water 10 times the weight of the raw materials for 12 hours. After soaking, the raw materials were decocted with water for three times, the decoctions were combined, concentrated, and freeze-dried to obtain powder B.

[0062] Comparative Example 4

[0063] The difference between this comparative example and Example 1 is that Angelica sinensis is replaced by Salvia miltiorrhiza, and Ginkgo biloba is replaced by Astragalus membranaceus.

[0064] Comparative Example 5

[0065] The difference between this comparative example and Example 1 is that the neutral protease is replaced by bromelain, and the ficin is replaced by pectinase.

[0066] Comparative Example 6

[0067] The difference between this comparative example and Example 1 is that the composite bacteria include Lactobacillus brevis, Lactobacillus plantarum and Bifidobacterium animalis. The dosage of Lactobacillus brevis is 10 9CFU / mL mixed solution B; the dosage of Lactobacillus plantarum is 10 7 CFU / mL mixed solution B; the dosage of animal Bifidobacterium is 10 8 CFU / mL mixed solution B.

[0068] Performance Testing

[0069] Scopolamine is an anticholinergic drug commonly used to treat motion sickness, pre-anesthetic medication, etc. It produces its pharmacological effects by blocking the action of acetylcholine. In research, scopolamine is also used as a tool to induce memory impairment to simulate certain types of cognitive impairment, including symptoms associated with Alzheimer's disease (especially Alzheimer's disease). The scopolamine-induced memory impairment model can be used to screen new drugs with the potential to improve cognitive function. It is used to support their application in the treatment of Alzheimer's disease.

[0070] Experimental subjects: Kunming mice, SPF grade.

[0071] Examples 1 to 5 and Comparative Examples 1 to 6 were used to prepare donkey-hide gelatin with the effect of improving Alzheimer's disease, which was prepared with distilled water to a desired concentration and stored in a refrigerator at 4° C. for later use.

[0072] Piracetam tablets: Tianjin Jinshi Pharmaceutical Co., Ltd., specification 0.4g.

[0073] Mice were randomly divided into groups according to body weight, 10 mice in each group, half male and half female, blank control group, model control group, positive control (1.4 g / kg piracetam tablets) group, and 2.5 g / kg donkey-hide gelatin group. Each group was given 20 ml / kg of the corresponding drug by gavage, and the blank control group and model control group were given an equal amount of normal saline by gavage. The drug was administered once a day for 35 consecutive days.

[0074] Scopolamine-induced memory acquisition disorder model: The animals were randomly divided into groups and dosed. One hour after the last dose, each group of mice was intraperitoneally injected with scopolamine hydrobromide injection 3 mg / kg, with an intraperitoneal injection volume of 10 ml / kg. The blank control group was intraperitoneally injected with an equal volume of normal saline. Ten minutes later, the jumping platform method was used for jumping platform training, and the test was repeated 24 hours later.

[0075] Learning performance and memory performance The jumping platform method was used to determine the effects of drugs on the learning performance and memory performance of mice. During the experiment, the mice were placed in the jumping platform instrument and adapted to the environment for 5 minutes. Then, the copper grid at the bottom was connected to an alternating current. The reaction time of the animals jumping onto the insulating platform after being electrically stimulated and the number of electric shocks within 5 minutes, that is, the number of errors, were recorded as learning performance. After 24 hours, the mice were placed on the platform, and the time (latency) for the first jump of the mice off the insulating platform and the number of electric shocks within 5 minutes (number of errors) were recorded as memory performance.

[0076] The results are shown in Table 1.

[0077] Table 1 Effects of scopolamine on learning and memory performance of mice with memory acquisition impairment

[0078]

[0079]

[0080] The above performance test results show that compared with the blank control group, the learning latency of the mice in the model control group was significantly prolonged, the memory latency was significantly shortened, and the number of errors within 5 minutes was significantly increased, indicating that the model was successfully established;

[0081] Compared with the model control group, the memory latency of the piracetam tablet group was significantly prolonged, the learning latency of Example 1 was significantly shortened, the memory latency was significantly prolonged, and the number of errors within 5 minutes was significantly reduced, indicating that the donkey-hide gelatin prepared by the present invention has an improving effect on the learning ability and memory ability of mice with memory acquisition disorders caused by scopolamine. In particular, the comprehensive performance of Example 1 is the most prominent, and the effect is better than that of the piracetam tablet group.

[0082] However, the comparative example does not adopt the necessary technical solution, resulting in that it is significantly inferior to the embodiment in the corresponding performance test.

[0083] In Comparative Example 1, the ratio of the raw materials was changed, and it can be seen that the effect decreased, which proves that the raw materials of the present invention have an important influence on the memory effect through a specific ratio.

[0084] The preparation methods of mixed powder A and mixed powder B in Comparative Examples 2 and 3 are different. It can be seen from the results that the memory effect is reduced, indicating that the active substances produced by the raw materials of the present invention through enzymatic hydrolysis and fermentation have an effect on improving the memory effect.

[0085] In Comparative Example 4, when Angelica sinensis was replaced by Salvia miltiorrhiza and Ginkgo biloba leaves were replaced by Astragalus membranaceus, the effect decreased, indicating that the two components have a synergistic effect in the system.

[0086] The types of enzymes and bacteria used in Comparative Examples 5 and 6 are different. From the results, it can be seen that the memory effect is reduced, indicating that the specific active substances produced by the raw materials of the present invention through specific enzymatic hydrolysis and fermentation have an effect on improving the memory effect.

[0087] The above experimental results further prove the importance of the technical solution defined in the present invention to its technical effect.

[0088] The above is a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A donkey-hide gelatin having the effect of improving Alzheimer's disease, characterized in that: The invention comprises the following raw materials in parts by weight: 15-30 parts of donkey-hide gelatin, 10-20 parts of angelica sinensis, 12-22 parts of atractylodes macrocephala, 5-15 parts of rhodiola rosea, 1-10 parts of ginkgo leaves, 7-17 parts of codonopsis pilosula and 1-14 parts of wolfberry.

2. The donkey-hide gelatin having the effect of improving Alzheimer's disease according to claim 1, characterized in that: The invention comprises the following raw materials in parts by weight: 15-20 parts of donkey-hide gelatin, 10-15 parts of angelica sinensis, 17-22 parts of atractylodes macrocephala, 5-10 parts of rhodiola rosea, 1-5 parts of ginkgo leaves, 8-12 parts of codonopsis pilosula and 9-14 parts of wolfberry.

3. The donkey-hide gelatin having the effect of improving Alzheimer's disease according to claim 2, characterized in that: The invention comprises the following raw materials in parts by weight: 17 parts of donkey-hide gelatin, 12 parts of angelica sinensis, 20 parts of atractylodes macrocephala, 7 parts of rhodiola rosea, 3 parts of ginkgo leaves, 11 parts of codonopsis pilosula and 9 parts of wolfberry.

4. A method for preparing donkey-hide gelatin having the effect of improving Alzheimer's disease according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) mixing angelica sinensis, atractylodes macrocephala, and rhodiola rosea and crushing them to obtain a mixture A, mixing the heavy mixture A with water, and adjusting the pH to 8.5 to 8.7 to obtain a mixed solution A; (2) adding alkaline protease to the mixed solution A, and performing enzymolysis at 45°C to 48°C for 40 to 60 minutes; inactivating the enzyme, adjusting the pH value to neutral, adding neutral protease, and performing enzymolysis at 45°C to 48°C for 60 to 70 minutes; inactivating the enzyme, and continuously adding ficin, and performing enzymolysis at 45°C to 48°C for 50 to 60 minutes; inactivating the enzyme to obtain an enzymolysis solution; taking the supernatant after centrifugation, and freeze-drying the solution to obtain powder A; (3) Ginkgo biloba leaves, Codonopsis pilosula and Lycium barbarum are mixed and crushed to obtain a mixture B, and the mixture B is mixed with water to obtain a mixed solution B; (4) adding the composite bacteria to the mixed solution B, fermenting at 30-35° C. for 15-20 h, sterilizing, and obtaining a fermentation solution; centrifuging the fermentation solution, taking the supernatant, and freeze-drying the solution to obtain a powder B; (5) After the donkey-hide gelatin is crushed, it is soaked in rice wine and stewed in water until it melts. Powder A and powder B are added, mixed evenly, and concentrated into a thick paste. After the thick paste is cooled, donkey-hide gelatin with the effect of improving Alzheimer's disease is obtained.

5. The method for preparing donkey-hide gelatin having the effect of improving Alzheimer's disease according to claim 4, characterized in that: The dosage of the alkaline protease is 600-800u / mL of the mixed solution A; the dosage of the neutral protease is 1000-1200u / mL of the mixed solution A; and the dosage of the ficin is 800-900u / mL of the mixed solution A.

6. The method for preparing donkey-hide gelatin having the effect of improving Alzheimer's disease according to claim 4, characterized in that: The complex bacteria include Lactobacillus casei, Bifidobacterium bifidum and Bifidobacterium longum.

7. The method for preparing donkey-hide gelatin having the effect of improving Alzheimer's disease according to claim 6, characterized in that: The dosage of Lactobacillus casei is 10 9 ~10 10 CFU / mL mixed solution B; the dosage of Bifidobacterium bifidum is 10 7 ~10 8 CFU / mL mixed solution B; the dosage of Bifidobacterium longum is 10 8 ~10 9 CFU / mL mixed solution B.

8. The method for preparing donkey-hide gelatin having the effect of improving Alzheimer's disease according to claim 4, characterized in that: The pH was adjusted to 8.5-8.7 using 5-7 wt% NaOH solution.

9. The method for preparing donkey-hide gelatin having the effect of improving Alzheimer's disease according to claim 4, characterized in that: The pH value was adjusted to neutral using 7-9 wt% citric acid.

10. The method for preparing donkey-hide gelatin having the effect of improving Alzheimer's disease according to claim 4, characterized in that: After crushing the donkey-hide gelatin, add 5 to 8 times the mass of rice wine and soak for 12 to 15 hours, then stew in water until melted.

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